Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,582,671	G→A	R270K (AGA→AAA)	yhhZ →	putative endonuclease YhhZ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,582,671	0	G	A	100.0%	36.5 / NA	12	R270K (AGA→AAA)	yhhZ	putative endonuclease YhhZ
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (8/4);  total (8/4)

GAAGGATTCAGAGATACTGCGGAAAAACTGGGTGGAGATGCAATAAAAGGCTATGATCAAATACTAAATGAAAAAACAGCGGGCATAGCGATAGCAACAGCATCTATTCTTTTAACAAAGCGTTCTAATGTTGATACATATACAGAAATAAATAGTTACTTAGGCAAACTTAGAGGTCAACAAAAACTTCTTGATGGTATAGACATAATAGAAATAATATACATTAAGAGACCTTCAAAAGACTTAGCTAACTTACGAAAGGAGTTTAATAAAACTGTAAGAAAAAATTTTCTTATCAAACTTGCAAAAACCTCCGAAGCATCTGGAAGATTCAACGCCGAAGACCTTTTAAGAATGAGAAAGGGCAATGTTCCTCTAAATTATAATGTTCACCATAAACTATCTCTAGATGATGGTGGTACTAATGATTTCGAAAATTTAGTATTAATCGAAAACGA  >  NC_000913/3582442‑3582899                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                     gaagggatCAGCGATACTGAGGAAAAAATGGGAGGAGATGCAATAAAAGGCTATGATCAAATACTAAATGAAAAAACAGCGGGCATAGCGATAGCAACAGCATCTATTCTTTTAACAAAGCGTTCTAATGTTGATACATATACAGAAATAAATATTTACTTAGGCAAACTTAGAGGTCAACAAAAACTTCTTGATGTTATAGACATAATAGAAATAATATACATTAAGAAACCTTCAAAAGCCTTAGCTa                                                                                                                                                                                                                  >  2:285072/8‑250 (MQ=255)                       aaaaaCTGGGTGGAGATGCAATAAAAGGCTATGATCAAATACTAAATGAAAAAACAGCGGGCATAGCGATAGCAACAGCATCTATTCTTTTAACAAAGCGTTCTAATGTTGATACATATACAGAAATAAATAGTTACTTAGGCAAACTTAGAGGTCAACAAAAACTTCTTGATGGTATAGACATAATAGAAATAATATACATTAAGAAACCTTCAAAAGACTTAGCTAACTTACGAAAGGAGTTTAATaaa                                                                                                                                                                                           <  1:351215/251‑1 (MQ=255)                                                                            aCAGCGGGCATAGCGATAGCAACAGCATCTATTCTTTTAACAAAGCGTTCTAATGTTGATACATATACAGAAATAAATAGTTACTTAGGCAAACTTAGAGGTCAACAAAAACTTCTTGATGGTATAGACATAATAGAAATAATATACATTAAGAAACCTTCAAAAGACTTAGCTAACTTACGAAAGGAGTTTAATAAAACTGTAAGAAAAAATTTTCTTATCAAACTTGCAAAAACCTCCGAAGCATCTgg                                                                                                                                      >  1:327393/1‑251 (MQ=255)                                                                                    cATAGCGATAGCAACAGCATCTATTCTTTTAACAAAGCGTTCTAATGTTGATACATATACAGAAATAAATAGTTACTTAGGCAAACTTAGAGGTCAACAAAAACTTCTTGATGGTATAGACATAATAGAAATAATATACATTAAGAAACCTTCAAAAGACTTAGCTAACTTACGAAAGGAGTTTAATAAAACTGTAAGAAAAAATTTTCTTATCAAACTTGCAAAAACCTCCGAAGCATCTGGAAGATTc                                                                                                                               >  1:441777/1‑250 (MQ=255)                                                                                          gATAGCAACAGCATCTATTCTTTTAACAAAGCGTTCTAATGTTGATACATATACAGAAATAAATAGTTACTTAGGCAAACTTAGAGGTCAACAAAAACTTCTTGATGGTATAGACATAATAGAAATAATATACATTAAGAAACATTCAAAAGACTTAGCTAACTTACGAAAGGAGTTTAATAAAACTGTAAGAAAAAATTTTCTTATCAAACTTGCAAAAACCTCCGAAGCATCTGGAAGATTCAACGCCg                                                                                                                        >  2:392840/1‑251 (MQ=255)                                                                                                aaCAGCATCTATTCTTTTAACACAGAGTTCTAATGTTGATAAAGATACAGACATAAATAGTTACTTAGGCCAAATTAGAAGTCAACAAAAACTTCTTGATGGAATAGACATAATAGAAATAATATACATTAAGAAACCTTCAAAAGACTTAGCAAACTTACGATAGGAGTTTAATAAAACTGTAAGAAAAAATTTTCTTATCAACCTTGCACAAACCTCCGAAGCATCTGGAAGATTCAACGACGTAGAcc                                                                                                                  >  2:262263/1‑251 (MQ=255)                                                                                                               tttAACAAAGCGTTCTAATGTTGATACATATACAGAAATAAATAGTTACTTAGGCAAACTTAGAGGTCAACAAAAACTTCTTGATGGTATAGACATAATAGAAATAATATACATTAAGAAACCTTCAAAAGACTTAGCTAACTTACGAAAGGAGTTTAATAAAGCTGTAAGAAAAAATTTTCTTATCAAACTTGCAAAAACCTCCGAAGCATCTTGAAGATTCAGCGCGGAAGACCTTTTAAGAATgaga                                                                                                    <  2:64044/250‑1 (MQ=255)                                                                                                                                                          aGTTACTTAGGCAAACTTAGAGGTCAACAAAAACTTCTTGATGGTATAGACATAATAGAAATAATATACATTAAGAAACCTTCAAAAGACTTAGCTAACTTACGAAAGGAGTTTAATAAAACTGTAAGAAAAAATTTTCTTATCAAACTTGCAAAAACCTCCGAAGCATCTGGAAGATTCAACGCCGAAGACCTTTTAAGAATGAGAAAGGGCAATGTTCCTCTAAATTATAATGTTCACCATAAACTAt                                                         <  2:441777/250‑1 (MQ=255)                                                                                                                                                                     cAAACTTAGAGGTCAACACAAACTTCTTGATGGTATAGACATAATAGAAATAATATACATTAAGAAACCTTCAAAAGACTTAGCTAACTTACGAAAGGAGTTTAATAAAACTGTAAGAAAAAATTTTCTTATCAAACTTGCAAAAACCTCCGAAGCATCTGGAAGATTCAACGCCGAAGACCTTTTAAGAATGAGAAAGGGCAATGTTCCTCTAAATTATAATGTTCACCATAAACTATCTCTAGatgatg                                             >  1:134781/1‑251 (MQ=255)                                                                                                                                                                                 tCAACAAAAACTTCTTGATGGTATAGACATAATAGAAATAATATACATTAAGAAACCTTCAAAAGACTTAGCTAACTTACGAAAGGAGTTTAATAAAACTGTAAGAAAAAATTTTCTTATCAAACTTGCAAAAACCTCCGAAGCATCTGGAAGATTCAACGCCGAAGACCTTTTAAGAATGAGAAAGGGCAATGTTCCTCTAAATTATAATGTTCACCATAAACTATCTCTAGATGATGGTGGTACTAATg                                 >  1:445533/1‑251 (MQ=255)                                                                                                                                                                                         aaCTTCTTGATGGTATAGACATAATAGAAATAATATACATTAAGAAACCTTCAAAAGACTTAGCTAACTTACGAAAGGAGTTTAATAAAACTGTAAGAAAAAATTTTCTTATCAAACTTGCAAAAACCTCCGAAGCATCTGGAAGATTCAACGCCGAAGACCTTTTAAGAATGAGAAAGGGCAATGTTCCTCTAAATTATAATGTTCACCATAAACTATCTCTAGATGATGGTGGTACTAATGATTTCGaa                         <  1:416049/251‑1 (MQ=255)                                                                                                                                                                                                                 taGAAATAATATACATTAAGAAACCTTCAAAAGACTTAGCTAACTTACGAAAGGAGTTTAATAAAACTGTAAGAAAAAATTTTCTTATCAAACTTGCAAAAACCTCCGAAGCATCTGGAAGATTCAACGCCGAAGACCTTTTAAGAATGAGAAAGGGCAATGTTCCTCTAAATTATAATGTTCACCATAAACTATCTCTAGATGATGGTGGTACTAATGATTTCGAAAATTTAGTATTAATCGAAAACGa  >  2:234880/1‑250 (MQ=255)                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                     GAAGGATTCAGAGATACTGCGGAAAAACTGGGTGGAGATGCAATAAAAGGCTATGATCAAATACTAAATGAAAAAACAGCGGGCATAGCGATAGCAACAGCATCTATTCTTTTAACAAAGCGTTCTAATGTTGATACATATACAGAAATAAATAGTTACTTAGGCAAACTTAGAGGTCAACAAAAACTTCTTGATGGTATAGACATAATAGAAATAATATACATTAAGAGACCTTCAAAAGACTTAGCTAACTTACGAAAGGAGTTTAATAAAACTGTAAGAAAAAATTTTCTTATCAAACTTGCAAAAACCTCCGAAGCATCTGGAAGATTCAACGCCGAAGACCTTTTAAGAATGAGAAAGGGCAATGTTCCTCTAAATTATAATGTTCACCATAAACTATCTCTAGATGATGGTGGTACTAATGATTTCGAAAATTTAGTATTAATCGAAAACGA  >  NC_000913/3582442‑3582899

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑