Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,401,887	C→T	E457K (GAG→AAG)	abgB ←	p‑aminobenzoyl‑glutamate hydrolase subunit B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,401,887	0	C	T	97.5%	135.0 / NA	40	E457K (GAG→AAG)	abgB	p‑aminobenzoyl‑glutamate hydrolase subunit B
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (24/15);  minor base G (0/1);  total (24/16)
Fisher's exact test for biased strand distribution p-value = 4.00e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCGTCGTCACCATGAGTACGATAATCAAATAGATAAAGAGCAGAAAAGGATGGGGAACCTTGTTACCAATTCTTTCGACCCAGCCATAGAGCTTCCCGGATTGGGAGGACGACGGTATGGATGACATACTCATGGGCATTCCTCGGTTGTTGTGTTTGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGATAGGGCAGTGATACGGTTGC‑GTGTCCGTTACTTGCTGATGCTCTTGTTGGCATTCTTGCAATAGCCCTGAATCAAGGAAGAGATTGACTGTGGTTGCTGCCATAGTTTTCGCCGCCAGCAGCATTCCTTTATGAGCAATAGATGTTCGCCCCTGGCTAACCAGTTGCCACGTATGTAGCGGTGTACCGACGGCAAAACAGGGGCTGAAACACTGGGCAACAGGCAGTTTCCAACTGACGTCGCCGACATCAGTCGATGCCGCAAGCACGTTATCGGTGGCGGCATA  >  NC_000913/1401641‑1402130                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                    ccGTCGTCACCATGAGTACGATAATCAAATAGATAAAGAGCAGAAAAGGATGGGGAACCTTGTTACCAATTCTTTCGACCCAGCCATAGAGCTTCCCGGATTGGGAGGACGACGGTATGGATGACATACTCATGGGCATTCCTCGGTTGTTGTGTTTGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGATAGGGCAGTGATACGGTTGA‑GTGTCCGTTACTTGCTGATGCTTttgt                                                                                                                                                                                                                                                 >  1:182510/1‑251 (MQ=255)          ccATGAGTACGATAATCAAATAGATAAAGAGCAGAAAAGGATGGGGAACCTTGTTACCAATTCTTTCGACCCAGCCATAGAGCTTCCCGGATTGGGAGGACGACGGTATGGATGACATACTCATGGGCATTCCTCGGTTGTTGTGTTTGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGATAGGGCAGTGATACGGTTGC‑GTGTCCGTTACTTGCTGATGCTTTTGTTGGCATTCt                                                                                                                                                                                                                                        <  1:217149/251‑1 (MQ=255)            aTGAGTACGCTACTCAAATAGATAAAGAGAACACAAGGATGGGGAACCTTGTTACCAATTCTTTCGACCCAGCCATAGAGCTTCCCGGATTGGGAGGACGACGGTATGGATGACATACTCATGGGCATTCCTCGGTTGTTGTGTTTGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTTGGGTTAGGGCAGTGATACGGTTGC‑GGGTCCGTTACTTGCTGATGCTTTTGTTGGCATTCTTg                                                                                                                                                                                                                                      >  2:314484/1‑251 (MQ=255)                                gATAAAGAGCAGAAAAGGATGGGGAACCTTGTTACCAATTCTTTCGACCCAGCCATAGAGCTTCCCGGATTGGGAGGACGACGGTATGGATGACATACTCATGGGCATTCCTCGGTTGTTGTGTTTGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGATAGGGCAGTGATACGGTTGC‑GTGTCCGTTACTTGCTGATGCTTTTGTTGGCATTCTTGCAATAGCCCTGAATCAAgg                                                                                                                                                                                                                   >  1:425400/1‑250 (MQ=255)                                  tAAAGAGCAGAAAAGGATGGGGAACCTTGTTACCAATTCTTTCGACCCAGCCATAGAGCTTCCCGGATTGGGAGGACGACGGTATGGATGACATACTCATGGGCATTCCTCGGTTGTTGTGTTTGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGATAGGGCAGTGATACGGTTGC‑GTGTCCGTTACTTGCTGATGCTTTTGTTGGCATTCTTGCAATAGCCCTGAATCAAGGa                                                                                                                                                                                                                  >  2:299826/1‑249 (MQ=255)                                      gagCAGAAAAGGATGGGGAACCTTGTTACCAATTCTTTCGACCCAGCCATAGAGCTTCCCGGATTGGGAGGACGACGGTATGGATGACATACTCATGGGCATTCCTCGGTTGTTGTGTTTGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGATAGGGCAGTGATACGGTTGC‑GTGTCCGTTACTTGCTGATGCTTTTGTTGGCATTCTTGCAATAGCCCTGAATCAAGGAAGAGAt                                                                                                                                                                                                            <  2:297332/251‑1 (MQ=255)                                          aGAAACGGACGGGGAACCTTGCAACCAATTCTTTCGACCCAGCCATAGAGCTTCACGGATTGGGAGGACGACGGTATGGATGACATACTCATGGGCATTCCTCGGTTGTTGTGTTTGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGATCGGGCAGTGATACGGTTGC‑GTGTCCGTTACTTGCTGATGCTTTTGTTGGCATTCTTGCAATAGCCCTGAATCAAGGAAGAGATTGAc                                                                                                                                                                                                        >  2:199487/1‑251 (MQ=255)                                            aaaaGGATGGGGAACCTTGTTACCAATTCTTTCGACCCAGCCATAGAGCTTCCCGGATTGGGAGGACGACGGTATGGATGACATACTCATGGGCATTCCTCGGTTGTTGTGTTTGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGATAGGGCAGTGATACGGTTGC‑GTGTCCGTTACTTGCTGATGCTTTTGTTGGCATTCTTGCAATAGCCCTGAATCAAGGAAGAGATTGACtg                                                                                                                                                                                                      >  1:252109/1‑251 (MQ=255)                                               aGGATGGGGAACCTTGTTACCAATTCTTTCGACCCAGCCATAGAGCTTCCCGGATTGGGAGGACGACGGTATGGATGACATACTCATGGGCATTCCTCGGTTGTTGTGTTTGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGATAGGGCAGTGATACGGTTGC‑GTGTCCGTTACTTGCTGATGCTTTTGTTGGCATTCTTGCAATAGCCCTGAATCAAGGAAGAGATTGACTGTgg                                                                                                                                                                                                   <  1:81155/251‑1 (MQ=255)                                                ggATGGGGAACCTTGTTACCAATTCTTTCGACCCAGCCATAGAGCTTCCCGGATTGGGAGGACGACGGTATGGATGACATACTCATGGGCATTCCTCGGTTGTTGTGTTTGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGATAGGGCAGTGATACGGTTGC‑GTGTCCGTTACTTGCTGATGCTTTTGTTGGCATTCTTGCAATAGCCCTGAATCAAGGAAGAGATTGACTGTGGt                                                                                                                                                                                                  <  2:425400/251‑1 (MQ=255)                                                    ggggAACCTTATTGCCAATGCTTTCATCCCAGCCATAGAGCTTCCCGGATTGGGAGGACGACGGTATGGATGACATACTCAGGGGCATTCCTCGGTTGTTGTGTTTGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGATAGGGCAGTGATACTGTTGC‑GTGTCCGTTACTTGCTGATGCTTTTGTTGGCATTCTTTCAATAGCCCTTAATCAAGGAAGAGATTGACTGTGGTtgat                                                                                                                                                                                              <  2:34143/251‑3 (MQ=255)                                                      ggAAACTTGTTACCAATTCTTTCGCCCCAGCCATAGAGCTTCCCGGCTTGAGAGGACGACGGTATGGATGACATACTCATGGGCATTCCTCGGTTGTTGTGTTTGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCCCGTTTTTCGGGATAGGGCAGTGATACGGTTGC‑GTGTCCGTTACTTGCTGATGCTTTTGTTGGCATTCTTGCAATAGCCCTGAATCAAGGAAGAGATTGACTGTGGTtgctgc                                                                                                                                                                                            >  2:47865/1‑251 (MQ=255)                                                                   cAATTCTTTCGACCCAGCCATAGAGCTTCCCGGATTGGGAGGTCGACGGTATGGATGAAATACTCATGGGCATTCCTCGGTTGTTGTGTTTGCGTTGTTGTTGTTATTTTAAAGGGGACGGGGTCACGTTTTGCGGGATAGGGCAGTGATACGGTTGC‑GCGTCCGTTACTTGCTGATGCTTTTGTTGGCATTCTCGCAGTAGCCCTGAATCAAGGAAGCGATTGCCTTCGGTTGCTGCCATCGGTTTcgcg                                                                                                                                                                               >  1:17598/1‑250 (MQ=255)                                                                     aTTCTTTCGACCCAGCCATAGAGCTTCCCGGATTGGGAGGACGACGGTATGGATGACATACTCATGGGCATTCCTCGGTTGTTGTGTTTGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGATAGGGCAGTGATACGGTTGC‑GTGTCCGTTACTTGCTGATGCTTTTGTTGGCATTCTTGCAATAGCCCTGAATCAAGGAAGAGATTGACTGTGGTTGCTGCCATAGTTTTcgccgc                                                                                                                                                                             >  2:23489/1‑251 (MQ=255)                                                                      ttCTTTCGACCCAGCCATAGAGCTTCCCGGATTGGGAGGACGACGGTATGGATGACATACTCATGGGCATTCCTCGGTTGTTGTGTTTGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGATAGGGCAGTGATACGGTTGC‑GTGTCCGTTACTTGCTGATGCTTTTGTTGGCATTCTTGCAATAGCCCTGAATCAAGGAAGAGATTGACTGTGGTTGCTGCCATAGTTTTCgccgcc                                                                                                                                                                            >  1:55094/1‑251 (MQ=255)                                                                          ttCGACCCAGCCATAGAGCTTCCCGGATTGGGAGGACGACGGTATGGATGACATACTCATGGGCATTCCTCGGTTGTTGTGTTTGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGATAGGGCAGTGATACGGTTGC‑GTGTCCGTTACTTGCTGATGCTTTTGTTGGCATTCTTGCAATAGCCCTGAATCAAGGAAGAGATTGACTGTGGTTGCTGCCATa                                                                                                                                                                                        >  1:223669/1‑235 (MQ=255)                                                                          ttCGACCCAGCCATAGAGCTTCCCGGATTGGGAGGACGACGGTATGGATGACATACTCATGGGCATTCCTCGGTTGTTGTGTTTGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGATAGGGCAGTGATACGGTTGC‑GTGGCCGTTACTTGCTGATGCTTTTGTTGGCATTCTTGCAATAGCCCTGAATCAAGGAAGAGATTGACTGTGGTTGCTGCCATa                                                                                                                                                                                        <  2:223669/235‑1 (MQ=255)                                                                                      aTAGAGCTTCCCGGATTGGGAGGACGACGGTATGGATGACATACTCATGGGCATTCCTCGGTTGTTGTGTTTGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGATAGGGCAGTGATACGGTTGC‑GTGTCCGTTACTTGCTGATGCTTTTGTTGGCATTCTTGCAATAGCCCTGAATCAAGGAAGAGATTGACTGTGGTTGCTGCCATAGTTTTCGCCGCCAGCAGCATTCCTTTAt                                                                                                                                                            >  1:22972/1‑251 (MQ=255)                                                                                         gagCTTCCCGGATTGGGAGGACGACGGCATGGATGACATACTCATGGGCATTCCTCGGTTGTTGTGTTTGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGATAGGGCAGTGATACGGTTGC‑GTGTCCGTTACTTGCTGATGCTTTTGTTGGCATTCTTGCAATAGCCCTGAATCAAGGAAGAGATTGACTGTGGTTGCTGCCATAGTTTTCGCCGCCAGCAGCATTCCTTTATg                                                                                                                                                           >  1:345741/1‑249 (MQ=255)                                                                                                 cGGATTGGGAGGACGACGGTATGGATGACATACTCATGGGCATTCCTCGGTTGTTGTGTTTGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGATAGGGCAGTGATACGGTTGC‑GTGTCCGTTACTTGCTGATGCTTTTGTTGGCATTCTTGCAATAGCCCTGAATCAAGGAAGAGATTGACTGTGGTTGCTGCCATAGTTTTCGCCGCCAGCAGCATTCCTTTATGAGCAAt                                                                                                                                                     >  1:34321/1‑247 (MQ=255)                                                                                                 cGGATTGGGAGGACGACGGTATGGATGACATACTCATGGGCATTCCTCGGTTGTTGTGTTTGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGATAGGGCAGTGATACGGTTGC‑GTGTCCGTTACTTGCTGATGCTTTTGTTGGCATTCTTGCAATAGCCCTGAATCAAGGAAGAGATTGACTGTGGTTGCTGCCATAGTTTTCGCCGCCAGCAGCATTCCTTTATGAGCAAt                                                                                                                                                     >  2:334264/1‑247 (MQ=255)                                                                                                         gaggaCGACGGTATGGATGACATACTCATGGGCATTCCTCGGTTGTTGTGTTTGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGATAGGGCAGTGATACGGTTGC‑GTGTCCGTTACTTGCTGATGCTTTTGTTGGCATTCTTGCAATAGCCCTGAATCAAGGAAGAGATTGACTGTGGTTGCTGCCATAGTTTTCGCCGCCAGCAGCATTCCTTTATGAGCAATAGATGTTCGccc                                                                                                                                         <  1:23489/251‑1 (MQ=255)                                                                                                                    tATGGATGACATACTCATGGGCATTCCTCGGTTGTTGTGTTTGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGATAGGGCAGTGATACGGTTGC‑GTGTCCGTTACTTGCTGATGCTTTTGTTGGCATTCTTGCAATAGCCCTGAATCAAGGAAGAGATTGACTGTGGTTGCTGCCATAGTTTTCGCCGCCAGCAGCATTCCTTTATGAGCAATAGATGTTCGCCCCTGGCTAAcc                                                                                                                               >  1:219695/1‑250 (MQ=255)                                                                                                                          tGACATACTCATGGGCATTCCTCGGTTGTTGTGTTTGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGATAGGGCAGTGATACGGTTGC‑GTGTCCGTTACTTGCTGATGCTTTTGTTGGCATTCTTGCAATAGCCCTGAATCAAGGAAGAGATTGACTGTGGTTGCTGCCATAGTTTTCGCCGCCAGCAGCATTCCTTTATGAGCAATAGATGTTCGCCCCTGGCTAACCAGTTGcc                                                                                                                        >  1:286952/1‑251 (MQ=255)                                                                                                                              aTACTCATGGGCATTCCTCGGTTGTTGTGTTTGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGATAGGGCAGTGATACGGTTGC‑GTGTCCGTTACTTGCTGATGCTTTTGTTGGCATTCTTGCAATAGCCCTGAATCAAGGAAGAGATTGACTGTGGTTGCTGCCATAGTTTTCGCCGCCAGCAGCATTCCTTTATGAGCAATAGATGTTCGCCCCTGGCTAACCAGTTGCCACGt                                                                                                                    <  2:110237/251‑1 (MQ=255)                                                                                                                                            tccgctgttgttGTGTTTGCTTTGTTGTTGTTATTTTAAAGGGCAAGGTGTCACGTTTTTCGGGATAGGGCAGTGATACGGTTGCCGT‑TCCGTTACTTGCTGATGCTTTTGTTGGCAGTCTTGCAATAGACCTGAAGCAAGGAAGAGATTGACGGTGGTTGCTGCCATAGTTTTCGCCGCCATCTGCATTCCTTTATGAGCAATAGATGTTCTCCCCTGGCTCGCCAGTTGCCACGTATGTCGCGgttt                                                                                                        <  2:239497/243‑3 (MQ=255)                                                                                                                                             ccTCGGTTGTTGTGTTTGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGATAGGGCAGTGATACGGTTGC‑GTGTCCGTTACTTGCTGATGCTTTTGTTGGCATTCTTGCAATAGCCCTGAATCAAGGAAGAGATTGACTGTGGTTGCTGCCATAGTTTTCGCCGCTAGCAGCATTCCTTTATGAGCAATAGATGTTCGCCCCTGGCTAACCAGTTGCCACGTATGTAGCGGTGTAcc                                                                                                     >  1:411410/1‑251 (MQ=255)                                                                                                                                             ccTCGGTTGTTGTGTTTGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGATAGGGCAGTGATACGGTTGC‑GTGTCCGTTACTTGCTGATGCTTTTGTTGGCATTCTTGCAATAGCCCTGAATCAAGGAAGAGATTGACTGTGGTTGCTGCCATAGTTTTCGCCGCCAGCAGCATTCCTTTATGAGCAATAGATGTTCGCCCCTGGCTAACCAGTTGCCACGTATGTAGCGGTGTAcc                                                                                                     <  1:199487/251‑1 (MQ=255)                                                                                                                                                       tgtgTTTGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGATAGGGCAGTGATACGGTTGC‑GTGTCCGTTACTTGCTGATGCTTTTGTTGGCATTCTTGCAATAGCCCTGAATCAAGGAAGAGATTGACTGTGGTTGCTGCCATAGTTTTCGCCGCCAGCAGCATTCCTTTATGAGCAATAGATGTTCGCCCCTGGCTAACCAGTTGCCACGTATGTAGCGGTGTACCGACGGCaaa                                                                                            <  1:71582/250‑1 (MQ=255)                                                                                                                                                        gtgtTTGCGTTGTTGTTGTTATTTTAAAGGGGACGGTGTCACGTTTTTCGGGATAGGGCAGTGATACGGTTGC‑GTGTCAGTTACTTGCTGATACTTTTGTTGGCATTCTTGCAATAGCCATGAATCAAGGAAGAGATTGACTGTGGTTGCTGCCATCGTTTTCGCCGCCAGAAGAATTCCTTTATGAGCAATAGATGTTCGCCCCTGGCTCACCAGTTGACACGTATGTAGCGGTGTACCGACGGCAAAAc                                                                                          >  2:34501/1‑251 (MQ=255)                                                                                                                                                                        tgttATTTTAAAGGTGACGGTGTCACGTTTTTCGGGATAGGGCAGTGATACGGTTGC‑GTGTCCGTTACTTGCTGATGCTTTTGTTGGCATTCTTGCAATAGCCCTGAATCAAGGAAGAGATTGACTGTGGTTGCTGCCATAGTTTTCGCCGCCAGCAGCATTCCTTTATGAGCAATAGATGTTCGCCCCTGGCTAACCAGTTGCCACGTATGTAGCGGTGTACCGACGGCAAAACAGGGGCTGAAACACt                                                                           <  2:5131/250‑1 (MQ=255)                                                                                                                                                                                 aaaGGTGACGGTGTCACGTTTTTCGGGATAGGGCAGTGATACGGTTGC‑GTGTCCGTTACTTGCTGATGCTTTTGTTGGCATTCTTGCAATAGCCCTGAATCAAGGAAGAGATTGACTGTGGTTGCTGCCATAGTTTTCGCCGCCAGCAGCATTCCTTTATGAGCAATAGATGTTCGCCCCTGGCTAACCAGTTGCCACGTATGTAGCGGTGTACCGACGGCAAAACAGGGGCTGAAACACTGGGCAACAgg                                                                 >  1:456496/1‑251 (MQ=255)                                                                                                                                                                                   aGGTGACGGTGTCACGTTTTTCGGGATAGGGCAGTGATACGGTTGC‑GTGTCCGTTACTTGCTGATGCTGTTGTTGGCATTCTTGCAATAGCCCTGAATCAAGGAAGAGATTGACTGTGGTTGCTGCCATAGTTTTCGCCGCCAGCAGCATTCCTTTATGAGCAATAGATGTTCGCCCCTGGCTAACCAGTTGCCACGTATGTAGCGGTGTACCGACGGCAAAACAGGGGCTGAAACACTGGGCAACAGGCa                                                               <  2:395722/251‑1 (MQ=255)                                                                                                                                                                                     gTGACGGTGTCACGTTTTTCGGGATAGGGCAGTGATACGGTTGC‑GTGTCCGTTACTTGCTGATGCTTTTGTTGGCATTCTTGCAATAGCCCTGAATCAAGGAAGAGATTGACTGTGGTTGCTGCCATAGTTTTCGCCGCCAGCAGCATTCCTTTATGAGCAATAGATGTTCGCCCCTGGCTAACCAGTTGCCACGTATGTAGCGGTGTACCGACGGCAAAACAGGGGCTGAAACACTGGGCAACAGGCAGt                                                             >  1:85779/1‑251 (MQ=255)                                                                                                                                                                                      tGACGGTGTCACGTTTTTCGGGATAGGGCAGTGATACGGTTGC‑GTGTCCGTTACTTGCTGATGCTTTTGTTGGCATTCTTGCAATAGCCCTGAATCAAGGAAGAGATTGACTGTGGTTGCTGCCATAGTTTTCGCCGCCAGCAGCATTCCTTTATGAGCAATAGATGTTCGCCCCTGGCTAACCAGTTGCCACGTATGTAGCGGTGTACCGACGGCAAAACAGGGGCTGAAACACTGGGCAACAGGCAGtt                                                            >  2:267141/1‑251 (MQ=255)                                                                                                                                                                                                aCGTTTTTCGGGATAGGGCAGTGATACGGTTGC‑GTGTCCGTTACTTGCTGATGCTTTTGTTGGCATTCTTGCAATAGCCCTGAATCAAGGAAGAGATTGACTGTGGTTGCTGCCATAGTTTTCGCCGCCAGCAGCATTCCTTTATGAGCAATAGATGTTCGCCCCTGGCTAACCAGTTGCCACGTATGTAGCGGTGTACCGACGGCAAAACAGGGGCTGAAACACTGGGCAACAGGCAGTTTCCAACTGAc                                                  >  1:167674/1‑251 (MQ=255)                                                                                                                                                                                                    ttttCGGGATAGGGCAGTGATACGGTTGC‑GTGTCCGTTACTTGCTGATGCTTTTGTTGGCATTCTTGCAATAGCCCTGAATCAAGGAAGAGATTGACTGTGGTTGCTGCCATAGTTTTCGCCGCCAGCAGCATTCCTTTATGAGCAATAGATGTTCGCCCCTGGCTAACCAGTTGCCACGTATGTAGCGGTGTACCGACGGCAAAACAGGGGCTGAAACACTGGGCAACAGGCAGTTTCCAACTGACGTCg                                              >  2:382673/1‑251 (MQ=255)                                                                                                                                                                                                                    gTGATACGGTTGC‑GTGTCCGTTACTTGCTGATGCTTTTGTTGGCATTCTTGCAATAGCCCTGAATCAAGGAAGAGATTGACTGTGGTTGCTGCCATAGTTTTCGCCGCCAGCAGCATTCCTTTATGAGCAATAGATGTTCGCCCCTGGCTAACCAGTTGCCACGTATGTAGCGGTGTACCGACGGCAAAACAGGGGCTGAAACACTGGGCAACAGGCAGTTTCCAACTGACGTCGCCGACATCAGTCGATg                              <  2:22972/251‑1 (MQ=255)                                                                                                                                                                                                                               gC‑GTGTCCGTTACTTGCTGATGCTTTTGTTGGCATTCTTGCAATAGCCCTGAATCAAGGAAGAGATTGACTGTGGTTGCTGCCATAGTTTTCGCCGCCAGCAGCATTCCTTTATGAGCAATAGATGTTCGCCCCTGGCTAACCAGTTGCCACGTATGTAGCGGTGTACCGACGGCAAAACAGGGGCTGAAACACTGGGCAACAGGCAGTTTCCAACTGACGTCGCCGACATCAGTCGATGCCGCAAGCAc                    <  2:219695/250‑1 (MQ=255)                                                                                                                                                                                                                                                  gATGCTTTTGTTGGCATTCTTGCAATAGCCCTGAATCAAGGAAGAGATTGACTGTGGTTGCTGCCATAGTTTTCGCCGCCAGCAGCATTCCTTTATGAGCAATAGATGTTCGCCCCTGGCTAACCAGTTGCCACGTATGTAGCGGTGTACCGACGGCAAAACAGGGGCTGAAACACTGGGCAACAGGCAGTTTCCAACTGACGTCGCCGACATCAGTCGATGCCGCAATCACGTTATCGGTGGCGGCata  <  2:32350/250‑1 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                    CCGTCGTCACCATGAGTACGATAATCAAATAGATAAAGAGCAGAAAAGGATGGGGAACCTTGTTACCAATTCTTTCGACCCAGCCATAGAGCTTCCCGGATTGGGAGGACGACGGTATGGATGACATACTCATGGGCATTCCTCGGTTGTTGTGTTTGCGTTGTTGTTGTTATTTTAAAGGTGACGGTGTCACGTTTTTCGGGATAGGGCAGTGATACGGTTGC‑GTGTCCGTTACTTGCTGATGCTCTTGTTGGCATTCTTGCAATAGCCCTGAATCAAGGAAGAGATTGACTGTGGTTGCTGCCATAGTTTTCGCCGCCAGCAGCATTCCTTTATGAGCAATAGATGTTCGCCCCTGGCTAACCAGTTGCCACGTATGTAGCGGTGTACCGACGGCAAAACAGGGGCTGAAACACTGGGCAACAGGCAGTTTCCAACTGACGTCGCCGACATCAGTCGATGCCGCAAGCACGTTATCGGTGGCGGCATA  >  NC_000913/1401641‑1402130

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑