Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	617,063	G→A	G969G (GGG→GGA)	entF →	apo‑serine activating enzyme

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	617,063	0	G	A	100.0%	214.5 / NA	64	G969G (GGG→GGA)	entF	apo‑serine activating enzyme
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (26/38);  total (26/38)

ACGCCTGTGTGATTAACCAGGCGGCTGCCACCGGTGGTGATGCGCGTCAATTGGTGGGCTATCTGGTGTCGCAATCGGGCCTGCCGTTGGATACCAGCGCATTGCAGGCGCAGCTTCGTGAAACATTGCCACCACATATGGTACCGGTGGTTCTGCTGCAACTTCCACAGTTACCACTTAGCGCCAACGGCAAGCTGGATCGCAAAGCCTTACCGTTGCCTGAACTGAAGGCACAAGCGCCAGGGCGTGCGCCGAAAGCGGGCAGTGAAACGATTATCGCCGCGGCATTCTCGTCGTTGCTGGGGTGTGACGTGCAGGATGCCGATGCTGATTTCTTCGCGCTTGGCGGTCATTCGCTACTGGCAATGAAACTGGCAGCGCAGTTAAGTCGGCAGGTTGCCCGCCAGGTGACGCCGGGGCAAGTGATGGTCGCGTCAACTGTCGCCAAACTGGCAACGATTATTGATGCTGAAGAAGACAGCACCCGGC  >  NC_000913/616819‑617307                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                     ccGCCTGTGTGATTAACCAGGCGGCTGCCACCGGTGGTGATGCGCGTCAATTGGTGGGCTATCTGGTGTCGCAATCGGGCCTGCCGTTGGATACCAGCGCATTGCAGGCGCAGCTTCGTGAAACATTGCCACCACATATGGTACCGGTGGTTCTGCTGCAACTTCCACAGTTACCACTTAGCGCCAACGGCAAGCTGGATCGCAAAGCCTTACCGTTGCCTGAACTGAAGGCACAAGCGCCAGGACGTgcg                                                                                                                                                                                                                                                <  2:326332/250‑1 (MQ=255)   gCCTGTGTGATTAACCAGGGGGCTGCCCCCGGTGGTGATGCGCGTCAATTGGTGGGCTAGCTGGTGTCGCAATCGGGCCTGCCGTTGGATACCAGCGCATTGCAGGCGCAGCTTCGTGAAACATTGCCACCACATATGGTACCGGTGGTTCTGCTGCAACTTCCACAGTTACCACTTAGCGCCAACGGCAAGCTGGATCGCAAAGCCTTACCGTTGCCTGAACTGAAGGCACAAGCGCCAGGACGTGCGcc                                                                                                                                                                                                                                              <  2:255089/251‑1 (MQ=255)               aaCCAGGCGGCTGCCACCGGTGGTGATGCGCGTCAATTGGTGGGCTATCTGGTGTCGCAATCGGGCCTGCCGTTGGATACCAGCGCATTGCAGGCGCAGCTTCGGGAAACATCGCCACCACATATGGTACCGGTGGTTATGCTGCAACTTCCACAGTTACCAATTAGCGCCCACGGCAAGCTGGCTCGCACAGCCTCACCCTTGACTGAACTGAAGGCACAAACGCCAGGACGCGCGCCGAAAGcagga                                                                                                                                                                                                                                    >  2:339437/1‑245 (MQ=255)                aCCAGGCGGCTGCCACCGGTGGTGATGCGCGTCAATTGGTGGGCTATCTGGTGTCGCAATCGGGCCTGCCGTTGGATACCAGCGCATTGCAGGCGCAGCTTCGTGAAACATTGCCACCACATATGGTACCGGTGGTTCTGCTGCAACTTCCACAGTTACCACTTAGCGCCAACGGCAAGCTGGATCGCAAAGCCTTACCGTTGCCTGAACTGAAGGCACAAGCGCCAGGACGTGCGCCGAAAGCGGGCAGt                                                                                                                                                                                                                                 <  1:256127/251‑1 (MQ=255)                         ccgCCACCGGTGGTCATGCGCGTCAATTGGTGGGCTTTCTGGGTTCGCAAGCGGGCCTGCCGTTGGATACCAGCGCATTGCAGGCGCAGCTTCGTGAAACATTGCCACCACATATGGTACCGGTGGTTCTGCTGCAACTTCCACAGTTACCACTTAGCGCCAACGGCAAGCTGGATCGCAAAGCCTTACCGTTGCCTGAACTGAAGGCACAAGCGCCAGGACGTGCGCCGAAAGCGGGCAGTGAAACGAtt                                                                                                                                                                                                                        <  2:44824/249‑1 (MQ=255)                           gCCACCGGTGGTGATGCGCGTCAATTGGTGGGTTATCTGGTGTCGCAATCGGGCCTGCCGTTGGATACCAGCGCATTGCAGGCGCAGCTTCGTGAAACATTGCCACCACATATGGTACCGGTGGTTCTGCTGCAACTTCCACAGTTACCACTTAGCGCCAACGGCAAGCTGGATCGCAAAGCCTTACCGTTGCCTGAACTGAAGGCACAAGCGCCAGGACGTGCGCCGAAAGCGGGCAGTGAAACGATTAt                                                                                                                                                                                                                      <  2:108596/251‑1 (MQ=255)                           gCCACCGGTGGTGATGCGCGTCAATTGGTGGGCTATCTGGTGTCGCAATCGGGCCTGCCGTTGGATACCAGCGCATTGCAGGCGCAGCTTCGTGAAACATTGCCACCACATATGGTACCGGTGGTTCTGCTGCAACTTCCACAGTTACCACTTAGCGCCAACGGCAAGCTGGATCGCAAAGCCTTACCGTTGCCTGAACTGAAGGCACAAGCGCCAGGACGTGCGCCGAAAGCGGGCAGTGAAACGAtt                                                                                                                                                                                                                        <  2:452530/249‑1 (MQ=255)                           gCCACCGGTGGTGATGCGCGTCAATTGGTGGGCTATCTGGTGTCGCAATCGGGCCTGCCGTTGGATACCAGCGCATTGCAGGCGCAGCTTCGTGAAACATTGCCACCACATATGGTACCGGTGGTTCTGCTGCAACTTCCACAGTTACCACTTAGCGCCAACGGCAAGCTGGATCGCAAAGCCTTACCGTTGCCTGAACTGAAGGCACAAGCGCCAGGACGTGCGCCGAAAGCGGGCAGTGAAACGATTa                                                                                                                                                                                                                       <  1:137336/250‑1 (MQ=255)                                  gtggtgATGCGCGTCAATTGGTGGGCTATCTGGTGTCGCAATCGGGCCTGCCGTTGGATACCAGCGCATTGCAGGCGCAGCTTCGTGAAACATTGCCACCACATATGGTACCGGTGGTTCTGCTGCAACTTCCACAGTTACCACTTAGCGCCAACGGCAAGCTGGATCGCAAAGCCTTACCGTTGCCTGAACTGAAGGCACAAGCGCCAGGACGTGCGCCGAAAGCGGGCAGTGAAACGATTATCGCcgcg                                                                                                                                                                                                               >  1:317600/1‑251 (MQ=255)                                     gtgATGCGCGTCAATTGGTGGGCTATCTGGTGTCGCAATCGGGCCTGCCGTTGGATACCAGCGCATTGCAGGCGCAGCTTCGTGAAACATTGCCACCACATATGGTACCGGTGGTTCTGCTGCAACTTCCACAGTTACCACTTAGCGCCAACGGCAAGCTGGATCGCAAAGCCTTACCGTTGCCTGAACTGAAGGCACAAGCGCCAGGACGTGCGCCGAAAGCGGGCAGTGAAACGATTATCGCCGCGGc                                                                                                                                                                                                             >  1:23331/1‑250 (MQ=255)                                       gATGCGCGTCAATTGGTGGGCTATCTGGTGTCGCAATCGGGCCTGCCGTTGGATACCAGCGCATTGCAGGCGCAGCTTCGTGAAACATTGCCACCACATATGGTACCGGTGGTTCTGCTGCAACTTCCACAGTTACCACTTAGCGCCAACGGCAAGCTGGATCGCAAAGCCTTACCGTTGCCTGAACTGAAGGCACAAGCGCCAGGACGTGCGCCGAAAGCGGGCAGTGAAACGATTATCGCCGCGGCAtt                                                                                                                                                                                                          >  1:256263/1‑251 (MQ=255)                                               tCAATTGGTGGGCTATCTGGTGTCGCAATCGGGCCTGCCGTTGGATACCAGCGCATTGCAGGCGCAGCTTCGTGAAACATTGCCACCACATATGGTACCGGTGGTTCTGCTGCAACTTCCACAGTTACCACTTAGCGCCAACGGCAAGCTGGATCGCAAAGCCTTACCGTTGCCTGAACTGAAGGCACAAGCGCCAGGACGTGCGCCGAAAGCGGGCAGTGAAACGATTATCGCCGCGGc                                                                                                                                                                                                             <  1:235586/240‑1 (MQ=255)                                               tCAATTGGTGGGCTATCTGGTGTCGCAATCGGGCCTGCCGTTGGATACCAGCGCATTGCAGGCGCAGCTTCGTGAAACATTGCCACCACATATGGTACCGGTGGTTCTGCTGCAACTTCCACAGTTACCACTTAGCGCCAACGGCAAGCTGGATCGCAAAGCCTTACCGTTGCCTGAACTGAAGGCACAAGCGCCAGGACGTGCGCCGAAAGCGGGCAGTGAAACGATTATCGCCGCGGc                                                                                                                                                                                                             >  2:235586/1‑240 (MQ=255)                                                cAATTGGTGGGCTATCTGGTGTCGCAATCGGGCCTGCCGTTGGATACCAGCGCATTGCAGGCGCAGCTTCGTGAAACATTGCCACCACATATGGTACCGGTGGTTCTGCTGCAACTTCCACAGTTACCACTTAGCGCCAACGGCAAGCTGGATCGCAAAGCCTTACCGTTGCCTGGACTGAAGGCACAAGCGCCAGGACGTGCGCCGAAAGCGGGCAGTGAAACGATTATCGCCGCGGCATTCTCGTCGtt                                                                                                                                                                                                 >  2:151442/1‑251 (MQ=255)                                                        gggCTATCTGGTGTCGCAATCGGGCCTGCCGTTGGATACCAGCGCATTGCAGGCGCAGCTTCGTGAAACATTGCCACCACATATGGTACCGGTGGTTCTGCTGCAACTTCCACAGTTACCACTTAGCGCCAACGGCAAGCTGGATCGCAAAGCCTTACCGTTGCCTGAACTGAAGGCACAAGCGCCAGGACGTGCGCCGAAAGCGGGCAGTGAAACGATTATCGCCGCGGCATTCTCGTCGTTGCTgggg                                                                                                                                                                                          <  1:94136/250‑1 (MQ=255)                                                        gggCTATCTGGTGTCGCAATCGGGCCTGCCGTTGGATACCAGCGCATTGCAGGCGCAGCTTCGTGAAACATTGCCACCACATATGGTACCGGTGGTTCTGCTGCAACTTCCACAGTTACCACTTAGCGCCAACGGCAAGCTGGATCGCAAAGCCTTACCGTTGCCTGAACTGAAGGCACAAGCGCCAGGACGTGCGCCGAAAGCGGGCAGTGAAACGATTATCGCCGCGGCATTCTCGTCGTTGCTGGGgt                                                                                                                                                                                         <  2:386694/251‑1 (MQ=255)                                                         ggCTATCTGGTGTCGCAATCGGGCCTGCCGTTGGATACCAGCGCATTGCAGGCGCAGCTTCGTGAAACATTGCCACCACATATGGTACCGGTGGTTCTGCTGCAACTTCCACAGTTACCACTTAGCGCCAACGGCAAGCTGGATCGCAAAGCCTTACCGTTGCCTGAACTGAAGGCACAAGCGCCAGGACGTGCGCCGAAAGCGGGCAGTGAAACGATTATCGCCGCGGCATTCTCGTCGTTGCTGGGgtg                                                                                                                                                                                        >  1:383328/1‑251 (MQ=255)                                                             aTCTGGTGTCGCAATCGGGCCTGCCGTTGGATACCAGCGCATTGCAGGCGCAGCTTCGTGAAACATTGCCACCACATATGGTACCGGTGGTTCTGCTGCAACTTCCACAGTTACCACTTAGCGCCAACGGCAAGCTGGATCGCCAAGCCTTACCGTTGCCTGAACTGAAGGCACAAGCGCCAGGACGTGCGCCGAAAGCGGGCAGTGAAACGATTATCGCCGCGGCATTCTCGTCGTTGCTGGGGTGTGa                                                                                                                                                                                     >  2:266446/1‑250 (MQ=255)                                                                         aaTCGGGCCTGCCGTTGGATACCAGCGCATTGCAGGCGCAGCTTCGTGAAACATTGCCACCACATATGGTACCGGTGGTTCTGCTGCAACTTCCACAGTTACCACTTAGCGCCAACGGCAAGCTGGATCGCAAAGCCTTACCGTTGCCTGAACTGAAGGCACAAGCGCCAGGACGTGCGCCGAAAGCGGGCAGTGAAACGATTATCGCCGCGGCATTCTCGTCGTTGCTGGGGTGTGACGTGCAGGATGcc                                                                                                                                                                        <  1:133061/251‑1 (MQ=255)                                                                             gggCCTGCCGTTGGATACCAGCGCATTGCAGGCGCAGCTTCGTGAAACATTGCCACCACATATGGTACCGGTGGTTCTGCTGCAACTTCCACAGTTACCACTTAGCGCCAACGGCAAGCTGGATCGCAAAGCCTTACCGTTGCCTGAACTGAAGGCACAAGCGCCAGGACGTGCGCCGAAAGCGGGCAGTGAAACGATTATCGCCGCGGCATTCTCGTCGTTGCTGGGGTGTGACGTGCAGGATGCCGATg                                                                                                                                                                    >  2:131573/1‑251 (MQ=255)                                                                              ggCCTGCCGTTGGATACCAGCGCATTGCAGGCGCAGCTTCGTGAAACATTGCCACCACATATGGTACCGGTGGTTCTGCTGCAACTTCCACAGTTACCACTTAGCGCCAACGGCAAGCTGGATCGCAAAGCCTTACCGTTGCCTGAACTGAAGGCACAAGCGCCAGGACGTGCGCCGAAAGCGGGCAGTGAAACGATTATCGCCGCGGCATTCTCGTCGTTGCTGGGGTGTGACGTGCAGGATGCCGATGc                                                                                                                                                                   >  1:55523/1‑251 (MQ=255)                                                                               gCCTGCCGTTGGATACCAGCGCATTGCAGGCGCAGCTTCGTGAAACATTGCCACCACATATGGTACCGGTGGTTCTGCTGCAACTTCCACAGTTACCACTTAGCGCCAACGGCAAGCTGGATCGCAAAGCCTTACCGTTGCCTGAACTGAAGGCACAAGCGCCAGGACGTGCGCCGAAAGCGGGCAGTGAAACGATTATCGCCGCGGCCTTCTCGTCGTTGCTGGGGGGTGACGTGCAGGATGCCGATGCt                                                                                                                                                                  >  2:93588/1‑251 (MQ=255)                                                                                    ccGTTGGATACCAGCGCATTGCAGGCGCAGCTTCGTGAAACATTGCCACCACATATGGTACCGGTGGTTCTGCTGCAACTTCCACAGTTACCACTTAGCGCCAACGGCAAGCTGGATCGCAAAGCCTTACCGTTGCCTGAACTGAAGGCACAAGCGCCAGGACGTGCGCCGAAAGCGGGCAGTGAAACGATTATCGCCGCGGCATTCTCGTCGTTGCTGGGGTGTGACGTGCAGGATGCCGATGCTGAttt                                                                                                                                                             >  1:206988/1‑251 (MQ=255)                                                                                        tGGATACCAGCGCATTGCAGGCGCAGCTTCGTGAAACATTGCCACCACATATGGTACCGGTGGTTCTGCTGCAACTTCCACAGTTACCACTTAGCGACAACGGCAAGCTGGATCGCAAAGCCTTACCGTTGCCTGAACTGAAGGCACAAGCGCCAGGACGTGCGCCGAAAGCGGGCAGTGAAACGATTATCGCCGCGGCATTCTCGTCGTTGCTGGGGTGGTACGTGCAGGATGCCGATGATGATttcttc                                                                                                                                                         >  2:162359/1‑251 (MQ=255)                                                                                                 gcgcATTGCAGGCGCAGCTTCGTGAAACATTGCCACCACATATGGTACCGGTGGTTCTGCTGCAACTTCCACAGTTACCACTTAGCGCCAACGGCAAGCTGGATCGCAAAGCCTTACCGTTGCCTGAACTGAAGGCACAAGCGCCAGGACGTGCGCCGAAAGCGGGCAGTGAAACGATTATCGCCGCGGCATTCTCGTCGTTGCTGGGGTGTGACGTGCAGGATGCCGATGCTGATTTCTTCGCGCTTGGc                                                                                                                                                <  1:384335/251‑1 (MQ=255)                                                                                                     aTTGCAGGCGCAGCTTCGTGAAACATTGCCACCACATATGGTACCGGTGGTTCTGCTGCAACTTCCACAGTTACCACTTAGCGCCAACGGCAAGCTGGATCGCAAAGCCTTACCGTTGCCTGAACTGAAGGCACAAGCGCCAGGACGTGCGCCGAAAGCGGGCAGTGAAACGATTATCGCCGCGGCATTCTCGTCGTTGCTGGGGTGTGACGTGCAGGATGCCGATGCTGATTTCTTCGCGCTTGGCGGTc                                                                                                                                            <  2:133412/251‑1 (MQ=255)                                                                                                          aGGCGCAGCTTCGTGAAACATTGCCACCACATATGGTACCGGTGGTTCTGCTGCAACTTCCACAGTTACCACTTAGCGCCAACGGCAAGCTGGATCGCAAAGCCTTACCGTTGCCTGAACTGAAGGCACAAGCGCCAGGACGTGCGCCGAAAGCGGGCAGTGAAACGATTATCGCCGCGGCATTCTCGTCGTTGCTGGGGTGTGACGTGCAGGATGCCGATGCTGATTTCTTCGCGCTTGGCGGTCATTCg                                                                                                                                       >  1:34988/1‑251 (MQ=255)                                                                                                             cgcAGCTTCGTGAAACATTGCCACCACATATGGTACCGGTGGTTCTGCTGCAACTTCCACAGTTACCACTTAGCGCCAACGGCAAGCTGGATCGCAAAGCCTTACCGTTGCCTGAACTGAAGGCACAAGCGCCAGGACGTGCGCCGAAAGCGGGCAGTGAAACGATTATCGCCGCGGCATTCTCGTCGTTGCTGGGGTGTGACGTGCAGGATGCCGATGCTGATTTCTTCGCGCTTGGCGGTCATTTGCt                                                                                                                                     >  2:241447/1‑250 (MQ=255)                                                                                                              gcAGCTTCGTGAAACATTGCCACCACATATGGTACCGGTGGTTCTGCTGCAACTTCCACAGTTACCACTTAGCGCCAACGGCAAGCTGGATCGCAAAGCCTTACCGTTGCCTGAACTGAAGGCACAAGCGCCAGGACGTGCGCCGAAAGCGGGCAGTGAAACGATTATCGCCGCGGCATTCTCGTCGTTGCTGGGGTGTGACGTGCAGGATGCCGATGCTGATTTCTTCGCGCTTGGCGGTCATTCGCTAc                                                                                                                                   >  2:379788/1‑251 (MQ=255)                                                                                                                aGCTTCGTGAAACATTGCCACCACATATGGTACCGGTGGGTCTGCTGCAACTTCCACAGTTACCACTTATCGCCAACGGCACGCTGGATCGCAAAGCCTTACCGTTGCCTGAACTGAAGGCACAAGCGCCAGGACGTGCGCCGAAAGCGGGCAGTGAAACGATTATCGCCGCGGCATTCTCGTAGTTGCTGGGGTGTGACGTGCAGGATGCCGATGCTGATTTCTTCGCGCTTGGCGGTCATTCGCTACTg                                                                                                                                 >  2:156817/1‑251 (MQ=255)                                                                                                                        gAAACATTGCCACCACATATGGTACCGGTGGTTCTGCTGCAACTTCCACAGTTACCACTTAGCGCCAACGGCAAGCTGGATCGCAAAGCCTTACCGTTGCCTGAACTGAAGGCACAAGCGCCAGGACGTGCGCCGAAAGCGGGCAGTGAAACGATTATCGCCGCGGCATTCTCGTCGTTGCTGGGGTGTGACGTGCAGGATGCCGATGCTGATTTCTTCGCGCTTGGCGGTCATTCGCTACTGGCAATGaa                                                                                                                         <  1:16124/251‑1 (MQ=255)                                                                                                                        gAAACATTGCCACCACATATGGTACCGGTGGTTCTGCTGCAACTTCCACAGTTACCACTTAGCGCCAACGGCAAGCTGGATCGCAAAGCCTTACCGTTGCCTGAACTGAAGGCACAAGCGCCAGGACGTGCGCCGAAAGCGGGCAGTGAAACGATTATCGCCGCGGCATTCTCGTCGTTGCTGGGGTGTGACGTGCAGGATGCCGATGCTGATTTCTTCGCGCTTGGCGGTCATTCGCTACTGGCAATGa                                                                                                                          <  1:261797/250‑1 (MQ=255)                                                                                                                         aaaCATTGCCACCACATATGGTACCGGTGGTTCTGCTGCAACTTCCACAGTTACCACTTAGCGCCAACGGCAAGCTGGATCGCAAAGCCTTACCGTTGCCTGAACTGAAGGCACAAGCGCCAGGACGTGCGCCGAAAGCGGGCAGTGAAACGATTATCGCCGCGGCATTCTCGTCGTTGCTGGGGTGTGACGTGCAGGATGCCGATGCTGATTTCTTCGCGCTTGGCGGTCATTCGCTACTGGCAATGaa                                                                                                                         >  2:9612/1‑250 (MQ=255)                                                                                                                                gCCACCACATATGGTACCGGTGGTTCTGCTGCAACTTCCACAGTTACCACTTAGCGCCAACGGCAAGCTGGATCGCAAAGCCTTACCGTTGCCTGAACTGAAGGCACAAGCGCCAGGACGTGCGCCGAAAGCGGGCAGTGAAACGATTATCGCCGCGGCATTCTCGTCGTTGCTGGGGTGTGACGTGCAGGATGCCGATGCTGATTTCTTCGCGCTTGGCGGTCATTCGCTACTGGCAATGAAACTGGc                                                                                                                   >  1:22926/1‑249 (MQ=255)                                                                                                                                gCCACCACATATGGTACCGGTGGTTCTGCTGCAACTTACACAGTTACCACTTCGCGCCAACGGCAAGCTGGATAGCAAAGCCTTACCGTTGCCTGAACTGAAGGACCAAGCGCCAGGACGTGCGCCGAAAGCGGGCAGTGAAACGATTATCGCCGCGGCATTCTCGTCGTTGCTGGGGTGTGACGTGCAGGATGCCGATGCTGATTTCTTCGCGCTTGGCGGTCATTCGCTACTGGCAATGAAACTGGc                                                                                                                   >  1:250822/1‑249 (MQ=255)                                                                                                                                  caccacATATGGTACCGGTGGTTCTGCTGCAACTTCCACAGTTACCACTTAGCGCCAACGGCAAGCTGGATCGCAAAGCCTTACCGTTGCCTGAACTGAAGGCACAAGCGCCAGGACGTGCGCCGAAAGCGGGCAGTGAAACGATTATCGCCGCGGCATTCTCGTCGTTGCTGGGGTGTGACGTGCAGGATGCCGATGCTGATTTCTTCGCGCTTGGCGGTCATTCGCTACTGGCAATGAAACTGGCAgcg                                                                                                               <  2:256263/251‑1 (MQ=255)                                                                                                                                       caTATGGTACCGGTGGTTCTGCTGCAACTTCCACAGTTACCACTTAGCGCCAACGGCAAGCTGGATCGCAAAGCCTTACCGTTGCCTGAACTGAAGGCACAAGCGCCAGGACGTGCGCCGAAAGCGGGCAGTGAAACGATTATCGCCGCGGCATTCTCGTCGTTGCTGGGGTGTGACGTGCAGGATGCCGATGCTGATTTCTTCGCGCTTGGCGGTCATTCGCTACTGGCAATGAAACTGGCAGCGCAGtt                                                                                                          <  1:292204/251‑1 (MQ=255)                                                                                                                                            gggaCCGGTGGTTCTGCTGCAACTTCCTCAGTTACCACTTAGCGCCAACGGCAAGCTGGATCGCAAAGCCTTACCGTTGCCTGAACTGAAGGCACAAGCGCCAGGACGTGCGCCGAAAGCGGGCAGTTAAACGATTATCGCCGCGGCATTCTCGTCGTTGCTGGGGTGTGACGTGCAGGATGCCGATGCTGATTTCTTCGCGCTTGGCGTTCATTCGCTACTGGCAATGAAACTGGCAGCGCAGTTAAGTc                                                                                                     <  2:342641/248‑1 (MQ=255)                                                                                                                                                 cGGTGGTTCTGCTGCAACTTCCACAGTTACCACTTAGCGCCAACGGCAAGCTGGATCGCAAAGCCTTACCGTTGCCTGAACTGAAGGCACAAGCGCCAGGACGTGCGCCGAAAGCGGGCAGTGAAACGATTATCGCCGCGGCATTCTCGTCGTTGCTGGGGTGTGACGTGCAGGATGCCGATGCTGATTTCTTCGCGCTTGGCGGTCATTCGCTACTGGCAATGAAACTGGCAGCGCAGTTAAGTCGGc                                                                                                  >  2:354666/1‑249 (MQ=255)                                                                                                                                                     ggtTCTGCTGCAACTTCCACAGTTACCCCTTAGCGCCAACGGCAAGCTGGATCGCAAAGCCTGACCGTTGCCTGAACTTAAGGCACAAGCGCCAGGACGTGCGCCGAAAGCGGGCAGTGAAACGATTATCGCCGCGGCATTCTCGTCGTTGCTGGGGTGTGACGTGCAGGATGCCGATGCTGATTTCTTCGCGCTTGGCGGTCATTCGCTACTGGCAATGAAACTGGCAGCGCAGTTAAGTCGGCAGGTTg                                                                                            <  2:43219/251‑1 (MQ=255)                                                                                                                                                        tCTGCTGCAACTTCCACAGTTACCACTTAGCGCCAACGGCAAGCTGGATCGCAAAGCCTTACCGTTGCCTGAACTGAAGGCACAAGCGCCAGGACGTGCGCCGAAAGCGGGCAGTGAAACGATTATCGCCGCGGCATTCTCGTCGTTGCTGGGGTGTGACGTGCAGGATGCCGATGCTGATTTCTTCGCGCTTGGCGGTCATTCGCTACTGGCAAGGAAACTGGCAGCGCAGTTAAGTCGGCAGGTTGcc                                                                                          <  1:173849/250‑1 (MQ=255)                                                                                                                                                                            tACCACTTAGCGCCAACGGCAAGCTGGATCGCAAAGCCTTACCGTTGCCTGAACTGAAGGCACAAGCGCCAGGACGTGCGCCGAAAGCGGGCAGTGAAACGATTATCGCCGCGGCATTCTCGTCGTTGCTGGGGTGTGACGTGCAGGATGCCGATGCTGATTTCTTCGCGCTTGGCGGTCATTCGCTACTGGCAATGAAACTGGCAGCGCAGTTAAGTCGGCAGGTTGCCCGCCAGGTGACGCCGGGGCa                                                                      <  2:58438/250‑1 (MQ=255)                                                                                                                                                                                   tAGCGCCAACGGCAAGCTGGATCGCAAAGCCTTACCGTTGCCTGAACTGAAGGCACAAGCGCCAGGACGTGCGCCGAAAGCGGGCAGTGAAACGATTATCGCCGCGGCATTCTCGTCGTTGCTGGGGTGTGACGTGCAGGATGCCGATGCTGATTTCTTCGCGCTTGGCGGTCATTCGCTACTGGCAATGAAACTGGCAGCGCAGTTAAGTCGGCAGGTTGCCCGCCAGGTGACGCCGGGGCAAGTGAt                                                                <  1:207023/249‑1 (MQ=255)                                                                                                                                                                                    cgcgcCAACGGCACGCTTGATCGCAAATCCGTACCTTTGCCTGAACTGAAGGCACAAGCGCCAGGACGTGCGCCGAAAGCGGGCAGTGGATCGATTATCGCCGCGGCATTCTCTTCGTTGCTGGGGTGTGACGTGCAGGATGCCGATGCTGATTTCTTCGCGCTTGGCGGTCATTCGCTACTGGCAATGAAACTGGCAGCGCAGTTAAGTCGGCAGGTTGCCCGCCAGGTGACGCCGGGGCAAGTGATgg                                                              <  2:233311/249‑1 (MQ=255)                                                                                                                                                                                    aGCGCCAACGGCAAGCTGGATCGCAAAGCCTTACCGTTGCCTGAACTGAAGGCACAAGCGCCAGGACGTGCGCCGAAAGCGGGCAGTGAAACGATTATCGCCGCGGCATTCTCGTCGTTGCTGGGGTGTGACGTGCAGGATGCCGATGCTGATTTCTTCGCGCTTGGCGGTCATTCGCTACTGGCAATGAAACTGGCAGCGCAGTTAAGTCGGCAGGTTGCCCGCCAGGTGACGCCGGGGCAAGTGATGGt                                                             >  2:347298/1‑251 (MQ=255)                                                                                                                                                                                      cgcCAACGGCAAGCTGGATCGCAAAGCCTTACCGTTGCCTGAACTGAAGGCACAAGCTCCAGGACGTGCGCCGAAAGCGGGCAGTGAAACGATTATCGCCGCGGCATTCTCGTCGTTGCTGGGGTGTGACGTGCAGGATGCCGATGCTGATTTCTTCGCGCTTGGCGGTCATTCGCTACTGGCAATGAAACTGGCAGCGCAGTTAAGTCGGCAGGTTGCCCGCCAGGTGACGCCGGGGCAAGTGATGGTcg                                                           <  1:356469/251‑1 (MQ=255)                                                                                                                                                                                       gcCAACGGCAAGCTGGATCGCAAAGCCTTACCGTTGCCTGAACTGAAGGCACAAGCGCCAGGACGTGCGCCGAAAGCGGGCAGTGAAACGATTATCGCCGCGGCATTCTCGTCGTTGCTGGGGTGTGACGTGCAGGATGCCGATGCTGATTTCTTCGCGCTTGGCGGTCATTCGCTACTGGCAATGAAACTGGCAGCGCAGTTAAGTCGGCAGGTTGCCCGCCAGGTGACGCCGGGGCAAGTGATGGTcgc                                                          <  1:347298/251‑1 (MQ=255)                                                                                                                                                                                          aaCGGCAAGCTGGATCCCAAAGCCTTACCGTTGCCTGAGCTGACGGCACAAGCGCCAGGACGTGCGCCGAAAGCGGGCAGTGAAACGATTATCGCCGCGGCATTCTCGTCGTTGCTGGGGTGTGACGTGCAGGATGCCGATGCTGATTTCTTCGCGCTTGGCGGTCATTCGCTACTGGCAATGAAACTGGCAGCGCAGTTAAGTCGGCAGGTTGCCCGCCAGGGTACGCCGGGGCAAGTGATGGTCGCGTc                                                       <  2:225713/251‑1 (MQ=255)                                                                                                                                                                                           actgCAAGCTGAATCGCAAAGCCTTACCGTTGCCTGAACTGAAGGCACAAGCGCAAGGACGTGCGCCGAAAGCGGGCAGTGAAACGATTATCGCCGCGGCATTCTCGTCGTTGCTGGGGTGTGACGTGCAGGATGCCGATGCTGATTTCTTCGCGCTTGGCGGTCATTCGCTACTGGCAATGAAACTGGCAGCGCAGTTAAGTCGGCAGGTTGCCCGCCAGGTGACTCCGGGGCAAGTGATGGTCGCGTCa                                                      <  2:94041/248‑1 (MQ=255)                                                                                                                                                                                           aCGGCAAGCTGGATCGCAAAGCCTTACCGTTGCCTGAACTGCAGGCACAAGCGCCAGGACGTGCGCCGAAAGCGGGCAGTGAAACGATTATCGCCGCGGCATTCTCGTCGTTGCTGGGGTGTGACGTGCAGGATGCCGATGCTGATTTCTTCGCGCTTGGCGGTCATTCGCTACTGGCAATGAAACTGGCAGCGCAGTTAAGTCGGCAGGTTGCCCGCCAGGGGACGCCGGGGCAAGTGATGGTCGCGTc                                                       >  1:374277/1‑250 (MQ=255)                                                                                                                                                                                            cGGCAAGCTGGATCGCAAAGCCTTACCGTTGCCTGAACTGAAGGCACAAGCGCCAGGACGTGCGCCGAAAGCGGGCAGTGAAACGATTATCGCCGCGGCATTCTCGTCGTTGCTGGGGTGTGACGTGCAGGATGCCGATGCTGATTTCTTCGCGCTTGGCGGTCATTCGCTACTGGCAATGAAACTGGCAGCGCAGTTAAGTCGGCAGGTTGCCCGCCAGGTGACGCCGGGGCAAGTGATGGTCGCGTCa                                                      >  2:297600/1‑250 (MQ=255)                                                                                                                                                                                                aagatGGATCGCAAAGCCTTACCGTTGCCTGAACTGAAGGCACAAGCGCCAGGACGTGCGCCGAAAGCGGGCAGTGAAACGATTATCGCCGCGGCATTCTCGTCGTTGCTGGGGTGTGACGTGCAGGATGCCGATGCTGATTTCTTCGCGCTTGGCGGTCATTCGCTACTGGCAATGAAACTGGCAGCGCCGTTAAGTCGGCAGGTTGCCCGCCAGGTAACGCCGGGGCAAGTGATGGTCGCGTCAAATGt                                                 >  2:8292/5‑251 (MQ=255)                                                                                                                                                                                                  gCTGGATCGCAAAGCCTTACCGTTGCCTGAACTGAAGGCACAAGCGCCAGGACGTGCGCCGAAAGCGGGCAGTGAAACGATTATCGCCGCGGCATTCTCGTCGTTGCTGGGGTGTGACGTGCAGGATGCCGATGCTGATTTCTTCGCGCTTGGCGGTCATTCGCTACTGGCAATGAAACTGGCAGCGCAGTTAAGTCGGCAGGTTGCCCGCCAGGTGACGCCGGGGCAAGTGATGGTCGCGTCAACTGTCg                                               <  2:379215/251‑1 (MQ=255)                                                                                                                                                                                                  gCTGGATCGCAAAGCCTTACCGTTGCCTGAACTGAAGGCACAAGCGCCAGGACGTGCGCCGAAAGCGGGCAGTGAAACGATTATCGCCGCGGCATTCTCGTCGTTGCTGGGGTGTGACGTGCAGGATGCCGATGCTGATTTCTTCGCGCTTGGCGGTCATTCGCTACTGGCAATGAAACTGGCAGCGCAGTTAAGTCGGCAGGTTGCCCGCCAGGTGACGCCGGGGCAAGTGATGGTCGCGTCAACTGTCg                                               <  2:241001/251‑1 (MQ=255)                                                                                                                                                                                                            aaaGCCTTACCGTTGCCTGAACTGAAGGCACAAGCGCCAGGACGTGCGCCGAAAGCGGGCAGTGAAACGATTATCGCCGCGGCATTCTCGTCGTTGCTGGGGTGTGACGTGCAGGATGCCGATGCTGATTTCTTCGCGCTTGGCGGTCATTCGCTACTGGCAATGAAACTGGCAGCGCAGTTAAGTCGGCAGGTTGCCCGCCAGGTGACGCCGGGGCAAGTGATGGTCGCGTCAACTGTCGCCAAACTGGc                                     >  1:317180/1‑251 (MQ=255)                                                                                                                                                                                                                       gTTGCCTGAACTGAAGGCACAAGCGCCAGGACGTGCGCCGAAAGCGGGCAGTGAAACGATTATCGCCGCGGCATTCTCGTCGTTGCTGGGGTGTGACGTGCAGGATGCCGATGCTGATTTCTTCGCGCTTGGCGGTCATTCGCTACTGGCAATGAAACTGGCAGCGCAGTTAAGTCGGCAGGTTGCCCGCCAGGTGGCGCCGTGTCAAGTGATGGTCGCGTCAACTGTCGCCAAACTGGCAACGATTATTg                          <  1:286135/251‑1 (MQ=255)                                                                                                                                                                                                                               aactgaaGGCACAAGCGCCAGGACGTGCGCCGAAAGCGGGCAGTGAAACGATTATCGCCGCGGCATTCTCGTCGTTGCTGGGGTGTGACGTGCAGGATGCCGATGCTGATTTCTTCGCGCTTGGCGGTCATTCGCTACTGGCAATGAAACTGGCAGCGCAGTTAAGTCGGCAGGTTGCCCGCCAGGTGACGCCGGGGCAAGTGATGGTCGCGTCAACTGTCGCCAAACTGGCAACGATTATTGATGCTgaa                  <  1:131573/251‑1 (MQ=255)                                                                                                                                                                                                                                actnaaGGCACAAGCGCCAGGACGTGCGCCGAAAGCGGGCAGTGAAACGATTATCGCCGCGGCATTCTCGTCGTTGCTGGGGTGTGACGTGCAGGATGCCGATGCTGATTTCTTCGCGCTTGGCGGTCATTCGTTACTGGCAATGAAACTGGCAGCGCAGTTAAGTCGGCAGGTTGCCCGCCAGGTGACGCCGGGGCAAGTGATGGTCGCGTCAACTGTCGCCAAACTGGCAACGATTATTGATGCTgaag                 <  2:414702/251‑1 (MQ=255)                                                                                                                                                                                                                                      ggCACAAGCGCCAGGACGTGCGCCGAAAGCGGGCAGTGAAACGATTATCGCCGCGGCATTCTCGTCGTTGCTGGGGTGTGACGTGCAGGATGCCGATGCTGATTTCTTCGCGCTTGGCGGTCATTCGCTACTGGCAATGAAACTGGCAGCGCAGTTAAGTCGGCAGGTTGCCCGCCAGGTGACGCCGGGGCAAGTGATGGTCGCGTCAACTGTCGCCAAACTGGCAACGATTATTGATGCTGAAGAAGACa           <  2:373534/251‑1 (MQ=255)                                                                                                                                                                                                                                       gCACAAGCGCCAGGACGTGCGCCGAAAGCGGGCAGTGAAACGATTATCGCCGCGGCATTCTCGTCGTTGCTGGGGTGTGACGTGCAGGATGCCGATGCTGATTTCTTCGCGCTTGGCGGTCATTCGCTACTGGCAATGAAACTGGCAGCGCAGTTAAGTCGGCAGGTTGCCCGCCAGGTGACGCCGGGGCAAGTGATGGGCGCGTCAACTGTCGCCAAACTGGCAACGATTATTGATGCTGAAGAAGACAg          <  1:454476/251‑1 (MQ=255)                                                                                                                                                                                                                                           aaGCGCCAGGACGTGCGACGAAAGCGGGCAGTGAAACGATTATCGCCGCGGCATTCTCGTCGTTGCTGGGGTGTGACGTGCAGGATGCCGATGCTGATTTCTTCGCGCTTGGCGGTCATTCGCTACTGGCAATGAAACTGGCAGCGCAGTTAAGTCGGCAGGTTGCCCGCCAGGTGACGCCGGGGCAAGTGATGGTCGCGTCAACTGTCTCCAAACTGGCAACGATTATTGATGCTGAAGAAGACAGCAcc      <  1:156817/251‑1 (MQ=255)                                                                                                                                                                                                                                             gcgcCAGGACGTGCGCCGAAAGCGGGCAGTGAAACGATTATCGCCGCGGCATTCTCGTCGTTGCTGGGGTGTGACGTGCAGGATGCCGATGCTGATTTCTTCGCGCTTGGCGGTCATTCGCTACTGGCAATGAAACTGGCAGCGCAGTTAAGTCGGCAGGTTGCCCGCCAGGTGACGCCGGGGCAAGTGATGGTCGCGTCAACTGTCGCCAAACTGGCAACGATTATTGATGCTGAAGAAGACAGCACCCg    <  1:93588/251‑1 (MQ=255)                                                                                                                                                                                                                                             gcgcCAGGACGTGCGCCGAAAGCGGGCAGTGAAACGATTATCGCCGCGGCATTCTCGTCGTTGCTGGGGTGTGACGTGCAGGATGCCGATGCTGATTTCTTCGCGCTTGGCGGTCATTCGCTACTGGCAATGAAACTGGCAGCGCAGTTAAGTCGGCAGGTTGCCCGCCAGGTGACGCCGGGGCAAGTGATGGTCGCGTCAACTGTCGCCAAACTGGCAACGATTATTGATGCTGAAGAAGACAGCACCCg    <  1:266446/251‑1 (MQ=255)                                                                                                                                                                                                                                               gcCAGGACGTGCGCCGAAAGCGGGCAGTGAAACGATTATCGCCGCGGCATTCTCGTCGTTGCTGGGGTGTGACGTGCAGGATGCCGATGCTGATTTCTTCGCGCTTGGCGGTCATTCGCTACTGGCAATGAAACTGGCAGCGCAGTTAAGTCGGCAGGTTGCCCGCCAGGTGACGCCGGGGCAAGTGATGGTCGCGTCAACTGTCGCCAAACTGGCAACGATTATTGATGCTGAAGAAGACAGCACCCGGc  <  1:306230/251‑1 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                     ACGCCTGTGTGATTAACCAGGCGGCTGCCACCGGTGGTGATGCGCGTCAATTGGTGGGCTATCTGGTGTCGCAATCGGGCCTGCCGTTGGATACCAGCGCATTGCAGGCGCAGCTTCGTGAAACATTGCCACCACATATGGTACCGGTGGTTCTGCTGCAACTTCCACAGTTACCACTTAGCGCCAACGGCAAGCTGGATCGCAAAGCCTTACCGTTGCCTGAACTGAAGGCACAAGCGCCAGGGCGTGCGCCGAAAGCGGGCAGTGAAACGATTATCGCCGCGGCATTCTCGTCGTTGCTGGGGTGTGACGTGCAGGATGCCGATGCTGATTTCTTCGCGCTTGGCGGTCATTCGCTACTGGCAATGAAACTGGCAGCGCAGTTAAGTCGGCAGGTTGCCCGCCAGGTGACGCCGGGGCAAGTGATGGTCGCGTCAACTGTCGCCAAACTGGCAACGATTATTGATGCTGAAGAAGACAGCACCCGGC  >  NC_000913/616819‑617307

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑