Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,214,646	G→A	T277I (ACT→ATT)	bluF ←	blue light‑ and temperature‑regulated antirepressor BluF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,214,646	0	G	A	100.0%	87.7 / NA	27	T277I (ACT→ATT)	bluF	blue light‑ and temperature‑regulated antirepressor BluF
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (14/13);  total (14/13)

TGAATTTCAAGTGATGTACAGCATTTTATGATCGCCTGAATAATTGCCTGCCGTGGCCCACTTTTATGAACATTGGTAATCAATTCCTGACTGATTTTAATTCTGTCAGGCTGGAAGCGTGACAGGAGTAACAAACCAGCAAAACCTGCGCCAAAATGATCAATTGCTACACTGATACCAGCAGCCTTTAGCGATTTAATCGCTTCGGCAAACTCATCAAACCGAGATATGACTTCACTTTCAGTAAATTCAACGATGATTTGTTCAGGCACCAGAGCATTGGCCTTTATTTCATTAAGTAAAAAAGAGACTGCGTCAGGTTCGTTAACCAGGGTCATAGGTAATAGATTGATTGAAATCATTTTATCACCGAGCTCAAGTGCGTGTGCCATCGTGAATGCAAGCGCCTTACTTTTGAGATCCGCTGTGTAGATTTCCCCGTCTTTACGCTGCCCAACCGCTATGGCTGATGGGCTATCTTCATTTTT  >  NC_000913/1214403‑1214890                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                     tGAATTTCAAGTGATGTACAGCATTTTATGATCGCCTGAATAATTGCCTGCCGTGGCCCACTTTTATGAACATTGGTAATCAATTCCTGACTGATTTTAATTCTGTCAGGCTGGAAGCGTGACAGGAGTAACAAACCAGCAAAACCTGCGCCAAAATGATCAATTGCTACACTGATACCAGCAGCCTTTAGCGATTTAATCGCTTCGGCAAACTCATCAAACCGAGATATGACTTCACTTTCAATAAATTc                                                                                                                                                                                                                                               >  2:6740/1‑251 (MQ=255) tGAATTTCAAGTGATGTACAGCATTTTATGATCGCCTGAATAATTGCCTGCCGTGGCCCACTTTTATGAACATTGGTAATCAATTCCTGACTGATTTTAATTCTGTCAGGCTGGAAGCGTGACAGGAGTAACAAACCAGCAAAACCTGCGCCAAAATGATCAATTGCTACACTGATACCAGCAGCCTTTAGCGATTTAATCGCTTCGGCAAACTCATCAAACCGAGATATGACTTCACTTTCAATAAATTc                                                                                                                                                                                                                                               >  2:298953/1‑251 (MQ=255)                  aCAGCATTTTATCATCGCCTGAATAATTGCCTGCCGTGGCCCACTTTTATGAACATTGGTAATCAATTCCTGACTGATTTTAATTCTGTCAGGCTGGAAGCGTGACAGGAGTAACAAACCAGCAAAACCTGCGCCAAAATGATCAATTGCTACACTGATACCAGCAGCCTTTAGCGATTTAATCGCTTCGGCAAACTCATCAAACCGAGATATGACTTCACTTTCAATAAATTCAACGATGATTTGTTCa                                                                                                                                                                                                                               <  1:99730/250‑1 (MQ=255)                                      aaatagatgcctgctatgtcctccTTTTATGAACAGTGGTAGTCAATTCCTGACAGATTTTAAATCTGTCAGGCTGGAAGCGTGACAGGAGTAACAAACCAGCAAAACCTGCGCCAAAATGATCCATTGCTACTCTGATACCAGCAGCCTTTAGCTATTTACTCGCTTCGGCAAAGTCATCAAACCGACATATGACTTCACTTTGAATTAATTCAACAATGATTTGTTCTGGCAGCAGAGCCATGGCCttt                                                                                                                                                                                                          <  2:310797/228‑1 (MQ=255)                                        ataatTGCCTGCCGTGGCCCACTTTTATGAACATTGGTAATCAATTCCTGACTGATTTTAATTCTGTCAGGCTGGAAGCGTGACAGGAGTAACAAACCAGCAAAACCTGCGCCAAAATGATCAATTGCTACACTGATACCAGCAGCCTTTAGCGATTTAATCGCTTCGGCAAACTCATCAAACCGAGATATGACTTCACTTTCAATAAATTCAACGATGATTTGTTCAGGCACCAGAGCATTGGCCTTTAt                                                                                                                                                                                                        >  1:366693/1‑251 (MQ=255)                                                 tgccGTGGCCCACTTTTATGCACATTGGTAATCAATTCCTGACTGATTTTAATTCTGTCAGGCTGGAAGCGTGACAGGAGTAACAAACCAGCAAAACCTGCGCCAAAATGATCAATTGCTACACTGATACCAGCAGCCTTTAGCGATTTAATCGCTTCGGCAAACTCATCAAACCGAGATATGACTTCACTTTCAATAAATTCAACGATGATTTGTTCAGGCACCAGAGCATTGGCCTTTATTTCATTa                                                                                                                                                                                                 >  1:368617/1‑249 (MQ=255)                                                      tGGCCCACTTTTATGAACATTGGTAATCAATTCCTGACTGATTTTAATTCTGTCAGGCTGGAAGCGTGACAGGAGTAACAAACCAGCAAAACCTGCGCCAAAATGATCAATTGCTACACTGATACCAGCAGCCTTTAGCGATTTAATCGCTTCGGCACACTCATCAAACCGAGATATGACTTCACTTTCAATAAATTCAACGATGATTTGTTCAGGCACCAGAGCATTGGCCTTTATTTCATTAAGTaaa                                                                                                                                                                                           >  2:119765/1‑250 (MQ=255)                                                           cccTTTTATGAACATTGGTAATCAATTCCTGACTGATTTTAATTCTGTCAGGCTGGAAGCGTGACAGGAGTAACAAACCAGCAAAACCTGCGCCAAAATGATCAATTGCTACACTGATACCAGCAGCCTTTAGCGATTTAATCGCTTCGGCAAACTCATCAAACCGAGATATGACTTCACTTTCAATAAATTCAACGATGATTTGTTCAGGCACCAGAGCATTGGCCTTTATTTCATTAAGTAAAAAagag                                                                                                                                                                                     <  2:381831/249‑1 (MQ=255)                                                                          tgggaaTCAATTCCTGACTGATTTTAATTCTGTCAGGCTGGAAGCGTGACAGGAGTAACAAACCAGCAAAACCTGCGCCAAAATGATCAATTGCTACACTGATACCAGCAGCCTTTAGCGATTTAATCGCTTCGGCAAACTCATCAAACCGAGATATGACTTCACTTTCAATAAATTCAACGATGATTTGTTCAGGCACCAGAGCATTGGCCTTTATTTCATTAAGTAAAAAAGAGACTGCGTCAGGTTc                                                                                                                                                                       <  1:78985/246‑1 (MQ=255)                                                                          tGGTAATCAATTCCTGACTGATTTTAATTCTGTCAGGCTGGAAGCGTGACAGGAGTAACAAACCAGCAAAACCTGCGCCAAAATGATCAATTGCTACACTGATACCAGCAGCCTTTAGCGATTTAATCGCTTCGGCAAACTCATCAAACCGAGATATGACTTCACTTTCAATAAATTCAACGATGATTTGTTCAGGCACCAGAGCATTGGCCTTTATTTCATTAAGTAAAAAAGAGACTGCGTCAGGTTc                                                                                                                                                                       <  2:207141/250‑1 (MQ=255)                                                                           ggTAATCAATTCCTGACTGATTTTAATTCTGTCAGGCTGGAAGCGTGACAGGAGAAACAAACCAGCAAAACCTGCGCCAAAATGATCAATTGCTACACTGATACCAGCAGCCTTTAGCGATTTAATCGCTTCGACAAACTAATCAAACCGAGATATGACTTCACTTTCAATAAATTCAACGATGATTTGTTCAGGCACCAGAGCATTCGCCTTTATTTCATTAAGTAAAAAAGAGCCTGCGTCAGGTTCGt                                                                                                                                                                     >  2:315248/1‑251 (MQ=255)                                                                                                    aTTCTGTCAGGCTGGAAGCGTGACAGGAGTAACAAACCAGCAAAACCTGCGCCAAAATGATCAATTGCTACACTGATACCAGCAGCCTTTAGCGATTTAATCGCTTCGGCAAACTCATCAAACCGAGATATGACTTCACTTTCAATAAATTCAACGATGATTTGTTCAGGCACCAGAGCATTGGCCTTTATTTCATTAAGTAAAAAAGAGACTGCGTCAGGTTCGTTAACCAGGGTCATAGGTAATAgatt                                                                                                                                            >  1:315250/1‑251 (MQ=255)                                                                                                      tCTGTCAGGCTGGAAGCGTGACAGGAGTAACAAACCAGCAAAACCTGCGCCAAAATGATCAATTGCTACACTGATACCAGCAGCCTTTAGCGATTTAATCGCTTCGGCAAACTCATCAAACCGAGATATGACTTCACTTTCAATAAATTCAACGATGATTTGTTCAGGCACCAGAGCATTGGCCTTTATTTCATTAAGTAAAAAAGAGACTGCGTCAGGTTCGTTAACCAGGGTCATAGGTAATAgattg                                                                                                                                           <  1:1640/250‑1 (MQ=255)                                                                                                          tCAGGCTGGAAGCGTGACAGGAGTAACAAACCAGCAAAACCTGCGCCAAAATGATCAATTGCTACACTGATACCAGCAGCCTTTAGCGATTTAATCGCTTCGGCCCACTCATCAAACCGAGATATGACTTCACTTTCAATACATTCAACGATGATTTGTTCAGGCACCAGAGCATTGGCCTTTATTTCATTAAGTAAAAAAGAGACTGCGTCAGGTTCGTTAACCAGGGTCATAGGTAATAGattgattg                                                                                                                                       >  1:452816/1‑250 (MQ=255)                                                                                                             ggCTGGAAGCGTGACAGGAGTAACAAACCAGCAAAACCTGCGCCAAAATGATCAATTGCTCCACTGATACCAGCAGCCTTTAGCGATTTAATCGCTTCGGCAAACTCATCAAACCGAGATATGACTTCACTTTCAATAAATTCAACGATGATTTGTTCAGGCACCAGAGCATTGGCCTTTATTTCATTAAGTAAAAAAGAGACTGCGTCAGGTTCGTTAACCAGGGTCATAGGTAATAGATTGATTGAAAt                                                                                                                                   <  2:368617/251‑1 (MQ=255)                                                                                                                   aaGCGTGACAGGAGTAACAAAACAGCAAAACCTGCGCCAAAATGATCAATTGCTACACTGATACCAGCAGCCTTTAGCGATTTAATCGCTTCGGCAAACTCATCAAACCGAGATATGACTTCACTTTCAATAAATTCAACGATGATTTGTTCAGGCACCAGAGCATTGGCCTTTATTTCATTAAGTAAAAAAGAGACTGCGTCAGGTTCGTTAACCAGGGTCATAGGTAATAGATTGATTGAAATCAtttt                                                                                                                             >  2:392690/1‑251 (MQ=255)                                                                                                                           cAGGAGTAACAAACCAGCAAAACCTGCGCCAAAATGATCAATTGCTACACTGATACCAGCAGCCTTTAGCGATTTAATCGCTTCGGCAAACTCATCAAACCGAGATATGACTTCACTTTCAATAAATTCAACGATGATTTGTTCAGGCACCAGAGCATTGGCCTTTATTTCATTAAGTAAAAAAGAGACTGCGTCAGGTTCGTTAACCAGGGTCATAGGTAATAGATTGATTGAAATCATTTTATCACCGa                                                                                                                     <  2:355980/251‑1 (MQ=255)                                                                                                                                               aaCCTGCGCCAAAATGATCAATTGCTACACTGATACCAGCAGCCTTTAGCGATTTAATCGCTTCGGCAAACTCATCAAACCGAGATATGACTTCACTTTCAATAAATTCAACGATGATTTGTTCAGGCACCAGAGCATTGGCCTTTATTTCATTAAGTAAAAAAGAGACTGCGTCAGGTTCGTTAACCAGGGTCATAGGTAATAGATTGATTGAAATCATTTTATCACCGAGCTCAAGTGCGTGTGCCATc                                                                                                 <  2:255722/251‑1 (MQ=255)                                                                                                                                                            aTGATCAATTGCTACACTGATACCAGCAGCCTTTAGCGATTTAATCGCTTCGGCAAACTCATCAAACCGAGATATGACTTCACTTTCAATAAATTCAACGATGATTTGTTCAGGCACCAGAGCATTGGCCTTTATTTCATTAAGTAAAAAAGAGACTGCGTCAGGTTCGTTAACCAGGGTCATAGGTAATAGATTGATTGAAATCATTTTATCACCGAGCTCAAGTGCGTGTGCCATCGTGAATGCAAgcg                                                                                    <  1:6740/251‑1 (MQ=255)                                                                                                                                                                       cTACACTGATACCAGCAGCCTTTAGCGATTTAATCGCTTCGGCAAACTCATCAAACCGAGATATGACTTCACTTTCAATAAATTCAACGATGATTTGTACAGGCACCAGAGCATTGGCCTTTATTTCATTAAGTAAAAAAGAGACTGCGTCAGGTTCGTTAACCAGGGTCATAGGTAATAGATTGATTGAAATCATTTTATCACCGAGCTCAAGTGCGTGTGCCATCGTGAATGCAAGCGCCTTACTTTTg                                                                         >  2:273172/1‑251 (MQ=255)                                                                                                                                                                                                      aaTCGCTTCGGCAAACTCATCAAACCGAGATATGACTTCACTTTCAATAAATTCAACGATGAGTTGTTCAGGCACCAGAGCATTGGCCTTTATTTCATTAAGTAAAAAAGAGACTGCGTCAGGTTCGTTAACCAGGGTCATAGGTAATAGATTGATTGAAATCATTTTATCACCGAGCTCAAGTGCGTGTGCCATCGTGAATGCAAGCGCCTTACTTTTGAGATCCGCTGTGTAGATTTCCCCGTCTTTAc                                          <  2:140311/251‑1 (MQ=255)                                                                                                                                                                                                       aTCGCTTCGGCAAACTCATCAAACCGAGATATGACTTCACTTTCAATAAATTCAACGATGATTTGTTCAGGCACCAGAGCATTGGCCTTTATTTCATTAAGTAAAAAAGAGACTGCGTCAGGTTCGTTAACCAGGGTCATAGGTAATAGATTGATTGAAATCATTTTATCACCGAGCTCAAGTGCGTGTGCAATCGTGAATGCAAGCGCCTTACTTTTGAGATCCGCTGTGTAGATTTCCCCGTCTTTACg                                         >  1:275228/1‑251 (MQ=255)                                                                                                                                                                                                                 cAAACTCATCAAACCGAGATATGACTTCACTTTCAATAAATTCAACGATGATTTGTTCAGGCACCAGAGCATTGGCCTTTATTTCATTAAGTAAAAAAGAGACTGCGTCAGGTTCGTTAACCAGGGTCATAGGTAATAGATTGATTGAAATCATTTTATCACCGAGCTCAAGTGCGTGTGCCATCGTGAATGCAAGCGCCTTACTTTTGAGATCCGCTGTGTAGATTTCCCCGTCTTTACGCTGCCCAAcc                               <  2:366693/251‑1 (MQ=255)                                                                                                                                                                                                                      tcatcaAACCGAGATATGACTTCACTTTCAATAAATTCAACGATGATTTGTTCAGGCACCAGAGCATTGGCCTTTATTTCATTAAGTAAAAAAGAGACTGCGTCAGGTTCGTTAACCAGGGTCATAGGTAATAGATTGATTGAAATCATTTTATCACCGAGCTCAAGTGCGTGTGCCATCGTGAATGCAAGCGCCTTACTTTTGAGATCCGCTGTGTAGATTTCCCCGTCTTTACGCTGCCCAACCGCTAt                          >  2:75143/1‑251 (MQ=255)                                                                                                                                                                                                                           aaaCCGAGATATGACTTCACTTTCAATAAATTCAACGATGATTTGTTCAGGCACCAGAGCATTGGCCTTTATTTCATTAAGTAAAAAAGAGACTGCGTCAGGTTCGTTAACCAGGGTCATAGGTAATAGATTGATTGAAATCATTTTATCACCGAGCTCAAGTGCGTGTGCCATCGTGAATGCAAGCGCCTTACTTTTGAGATCCGCTGTGTAGATTTCCCCGTCTTTACGCTGCCCAACCGCTATGGCTg                     >  1:276894/1‑251 (MQ=255)                                                                                                                                                                                                                              ccGAGATATGACTTCACTTTCAATAAATTCAACGATGATTTGTTCAGGCACCAGAGCATTGGCCTTTATTTCATTAAGTAAAAAAGAGACTGCGTCAGGTTCGTTAACCAGGGTCATAGGTAATAGATTGATTGAAATCATTTTATCACCGAGCTCAAGTGCGTGTGCCATCGTGAATGCAAGCGCCTTACTTTTGAGATCCGCTGTGTAGATTTCCCCGTCTTTACGCTGCCCAACCGCTATGGCTGATg                  <  2:130869/251‑1 (MQ=255)                                                                                                                                                                                                                                              cTTTCAATAAATTCAACGATGATTTGTTCAGGCACCAGAGCATTGGCCTTTATTTCATTAAGTAAAAAAGAGACTGCGTCAGGTTCGTTAACCAGGGTCATAGGTAATAGATTGATTGAAATCATTTTATCACCGAGCTCAAGTGCGTGTGCCATCGTGAATGCAAGCGCCTTACTTTTGAGATCCGCTGTGTAGATTTCCCCGTCTTTACGCTGCCCAACCGCTATGGCTGATGGGCTATCTTCAttttt  >  1:425668/1‑251 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                     TGAATTTCAAGTGATGTACAGCATTTTATGATCGCCTGAATAATTGCCTGCCGTGGCCCACTTTTATGAACATTGGTAATCAATTCCTGACTGATTTTAATTCTGTCAGGCTGGAAGCGTGACAGGAGTAACAAACCAGCAAAACCTGCGCCAAAATGATCAATTGCTACACTGATACCAGCAGCCTTTAGCGATTTAATCGCTTCGGCAAACTCATCAAACCGAGATATGACTTCACTTTCAGTAAATTCAACGATGATTTGTTCAGGCACCAGAGCATTGGCCTTTATTTCATTAAGTAAAAAAGAGACTGCGTCAGGTTCGTTAACCAGGGTCATAGGTAATAGATTGATTGAAATCATTTTATCACCGAGCTCAAGTGCGTGTGCCATCGTGAATGCAAGCGCCTTACTTTTGAGATCCGCTGTGTAGATTTCCCCGTCTTTACGCTGCCCAACCGCTATGGCTGATGGGCTATCTTCATTTTT  >  NC_000913/1214403‑1214890

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑