Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,565,710	G→A	S291F (TCC→TTC)	glgP ←	glycogen phosphorylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,565,710	0	G	A	100.0%	132.6 / NA	42	S291F (TCC→TTC)	glgP	glycogen phosphorylase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (20/22);  total (20/22)

CAACCGGCCAGGTTTCCAGCGCCTCGCTCATCAGCGTGTGGTTAGTGTAGGAGAAGACCTGACAACACACCTCAAACGCGTCGTCCCAGCTAAATTGGTGCTCATCGATCAGCAGACGCATCATCTCAGGAATCGACAGTACCGGATGGGTATCATTGAGATGAATCGCGATTTTATCCGCCAGGTTATCGTAGGTTTTATGCAACTGATAATGGCGGCTTAAAATGTCCTGAATGGTCGAGGAAACCAGGAAGTATTCCTG‑ACGCAGGCGCAGCTCACGCCCGGAGTAGGTGGAGTCATCCGGATACAGTACGCGAGATACGTTCTCGGAGTGGTTTTTATCTTCCACTGCCGCGAAGTAGTCACCCTGGTTGAATTTACCGAGGTTAATTTCGCTACTGGCTTGCGCACTCCACAAACGCAGCGTGTTGGTCGCGTCGGTGTCGTAACCAGGGATTATCTGATCGTAAGCGACTCCCAGAA  >  NC_000913/3565468‑3565950                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                  cAACCGGCCAGGTTTCCAGCGCCTCGCTCATCAGCGTGTGGTTAGTGTAGGAGACGACCTGACAACACACCTCAAACGCGCCGTCCCAGCTAAATTGGTGCTCATCGATCAGCAGACGCATCATCTCAGGAATCGACAGTACCGGATGGGGATCATTGAGATGAATCGCGATTTTATCCGCCAGGTTATCGTAGGTTTTATGCAACTGATAATGGCGGCTTAAATTGTCCTGAATGGTCGAGAAAACCAgg                                                                                                                                                                                                                                           >  2:41306/1‑251 (MQ=255)     cGGCCAGGTTTCCAGCGCCTCGCTCATCAGCGTGTGGTTAGTGTATGAGAAGACCTGACAACACACCTCAAACGCGTCGTCCCAGCTAAATTGGTGCTCATCCATCAGCAGACGCATCAGCGCAGGAATCGACAGTACCGGATGGGTAACATTGAGATGAATCACGATTTTATCCGCCAGGTTATCGTAGGTTTTATGCAACTGATACTGGCGGCTTAAAATGTCCTGAATGGTCGAGAAATCCAGGAAGt                                                                                                                                                                                                                                       <  2:104627/251‑1 (MQ=255)                        tcgatcatcaGCGTGTGGATAGTGTAGGAGAAGACCTGACAACACACCTCCAACGCGTCGTCCCAGCTAAATTGGTGCTCATCGATCAGCAGACGCATCATCTCAGGAATCGACAGTACCGGATGGGTATCATTGAGATGAATCGCGATTTTATCCGCCAGGTTATCGTAGGTTTTATGCAACTGATAATGGCGGCTTAAAATGTCCTGAATGGTCGAGAAAACCAGGAAGTATTTCTG‑ACGAAggggc                                                                                                                                                                                                                     >  2:69148/5‑246 (MQ=255)                            tcatcaGCGTGTGGTTAGTGTAGGAGAAGACCTGACAACACACCTCAAACGCGTCGTCCCAGCTAAATTGGTGCTCATCGATCAGCAGACGCATCATCTCAGGAATCGACAGTACCGGATGGGTATCATTGAGATGAATCGCGATTTTATCCGCCAGGTTATCGTAGGTTTTATGCAACTGATAATGGCGGCTTAAAATGTCCTGAATGGTCGAGAAAACCAGGAAGTATTCCTG‑ACGCAGGCGCAGCTc                                                                                                                                                                                                                >  1:289148/1‑250 (MQ=255)                              atcaGCGTGTGGTTAGTGTAGGAGAAGACCTGACAACACACCTCAAACGCGTCGTCCCAGCTAAATTGGTGCTCATCGATCAGCAGACGCATCATCTCAGGAATCGACAGTACCGGATGGGTATCATTGAGATGAATCGCGATTTTATCCGCCAGGTTATCGTAGGTTTTATGCAACTGATAATGGCGGCTTAAAATGTCCTGAATGGTCGAGAAAACCAGGAAGTATTCCTG‑ACGCAGGCGCAGCTCACg                                                                                                                                                                                                             <  2:38439/251‑1 (MQ=255)                                caGCGTGTGGTTAGTGTAGGAGAAGACCTGACAACACACCTCAAACGCGTCGTCCCAGCTAAATTGGTGCTCATCGATCAGCAGACGCATCATCTCAGGAATCGACAGTACCGGATGGGTATCATTGAGATGAATCGCGATTTTATCCGCCAGGTTATCGTAGGTTTTATGCAACTGATAATGGCGGCTTAAAATGTCCTGAATGGTCGAGAAAACCAGGAAGTATTCCTG‑ACGCAGGCGCAGCTCACGcc                                                                                                                                                                                                           <  1:425422/251‑1 (MQ=255)                                     tgtgGTTAGTGTAGGAGAAGACCTGACAACACACCTCAAACGCGTCGTCCCAGCTAAATTGGTGCTCATCGATCAGCAGACGCATCATCTCAGGAATCGACAGTACCGGATGGGTATCATTGAGATGAATCGCGATTTTATCCGCCAGGTTATCGTAGGTTTTATGCAACTGATAATGGCGGCTTAAACTGTCCTGAATGGTCGAGAAAAACAGGAAGAATTCCTG‑ACGAAGGCGCAGCTCACGCCCgg                                                                                                                                                                                                        >  2:318426/1‑249 (MQ=255)                                            aGTGTAGGAGAAGACCTGACAACACACCTCAAACGCGTCGTCCCAGCTAAATTGGTGCTCATCGATCAGCAGACGCATCATCTCAGGAATCGACAGTACCGGATGGGTATCATTGAGATGAATCGCGATTTTATCCGCCAGGTTATCGTAGGTTTTATGCAACTGATAATGGCGGCTTAAAATGTCCTGAATGGTCGAGAAAACCAAGAAGTATTCTTGCAC‑CAGGCGCAGCTCACGCCCGGAGTAggggg                                                                                                                                                                                               >  2:314140/1‑248 (MQ=255)                                             gtgtAGGAGAAGACCTGACAACACACCTCAAACGCGTCGTCCCAGCTAAATTGGTGCTCATCGATCAGCAGACGCATCATCTCAGGAATCGACAGTACCGGATGGGTATCATTGAGATGAATCGCGATTTTATCCGCCAGGTTATCGTAGGTTTTATGCAACTGATAATGGCGGCTTAAAATGTCCTGAATGGTCGAGAAAACCAGGAAGTATTCCTG‑ACGCAGGCGCAGCTCACGCCCGGAGAAGGTggg                                                                                                                                                                                              >  1:403146/1‑250 (MQ=255)                                                    agaagaCCTGACAACACACCTCAAACGCGTCGTCCCAGCTAAATTGGTGCTCATCGATCAGCAGACGCATCATCTCAGGAATCGACAGTACCGGATGGGTATCATTGAGATGAATCGCGCTTTTATCCGCCAGGTTATCGTAGGTTTTATGCAACTGATAATGGCGGCTTAAAATGTACTGACTGGTCGAGAAAACCAGGAAGAATTCCTG‑ACGCAGGCGCAGCTCACGCCCGGAGTAGGTGGAGtcctcc                                                                                                                                                                                       >  2:213940/1‑251 (MQ=255)                                                                 acacctcTCAAACTCGTCGTCCCAGCTAAATTGGTGCTCATCGATCAGCAGACGCATCATCGCAGGAATCGACAGTACCGGATGGGTATCATTGAGATGAATCGCGATTTTATCCGCCAGGTTATCGTAGGTTTTATGCAACTGATAATGGCGGCTTAAAATGTCCTGAATGGTCGAGAAAACCAGGAAGTATTCCTG‑ACGCAGGCGCAGCTCACGCCCGGAGTAGGTGGAGTCATCCGGATACAGTAcgc                                                                                                                                                                          <  2:301047/245‑1 (MQ=255)                                                                         cAAACGCGTCGTCCCAGCTAAATTGGTGCTCATCGATCAGCAGACGCATCATCTCAGGAATCGACAGTACCGGATGGGTATCATTGAGATGAATCGCGATTTTATCCGCCAGGTTATCGTAGGTTTTATGCAACTGATAATGGCGGCTTAAAATGTCCTGAATGGTCGAGAAAACCATGAAGTATTCCTG‑ACGCAGGCGCAGCTCACGCCCGGAGTAGGTGGAGTCATCCGGATACAGTACGCGAGATACg                                                                                                                                                                  <  1:446728/251‑1 (MQ=255)                                                                              gcgTCGTCCCAGCTAAATTGGTGCTCATCGATCAGCAGACGCATCATCTCAGGAATCGACAGTACCGGATGGGTATCATTGAGATGAATCGCGATTTTATCCGCCAGGTTATCGTAGGTTTTATGCAACTGATAATGGCGGCTTAAAATGTCCTGAATGGTCGAGAAAACCAGGAAGTATTCCTG‑ACGCAGGCGCAGCTCACGCCCGGAGTAGGTGGAGTCATCCGGATACAGTACGCGAGATACGTtctc                                                                                                                                                             <  1:161794/251‑1 (MQ=255)                                                                                            aaaTTGGTGCTCATCGATCAGCAGACGCATCATCTCAGGAATCGACAGTACCGGATGGGTATCATTGAGATGAATCGCGATTTTATCCGCCAGGTTATCGTAGGTTTTATGCAACTGATAATGGCGGCTTAAAATGTCCTGAATGGTCGAGAAAACCAGGAAGTATTCCTG‑ACGCAGGCGCAGCTCACGCCCGGAGTAGGTGGAGTCATCCGGATACAGTACGCGAGATACGTTCTCGGAGTGGttttt                                                                                                                                                 <  1:78879/249‑1 (MQ=255)                                                                                                 ggTGCTCATCGATCAGCAGACGCATCATCTCAGGAATCGACAGTACCGGATGGGTATCATTGAGATGAATCGCGATTTTATCCGCCAGGTTATCGTAGGTTTTATGCAACTGATAATGGCGGCTTAAAATGTCCTGAATGGTCGAGAAAACCAGGAAGTATTCCTG‑ACGCAGGCGCAGCTCACGCCCGGAGTAGGTGGAGTCATCCGGATACAGTACGCGAGATACGTTCTCGGAGTGGTTTTTATCTTcc                                                                                                                                          <  2:401283/251‑1 (MQ=255)                                                                                                                 cagACGCATCATCTCAGGAATCGACAGTACCGGATGGGTATCATTGAGATGAATCGCGATTTTATCCGCCAGGTTATCGTAGGTTTTATGCAACTGATAATGGCGGCGTAAAATGTCCTGAATGGTCGAGAAAACCAGGAAGTATGCCTT‑ACGCAGGCGCAGCTCACGCCCGGAGTAGGTGGAGTCATCCGGATTCAGTACGCTAGATACGTTCTCGTAGTGTTTTTTATCTTCCACTGCCGCGAagtagt                                                                                                                          <  1:256833/251‑1 (MQ=255)                                                                                                                    aCGCATCAGCTCAGGAATCGACAGTACCGGATGGGTATCATTGAGATGAATCGCGATTTTATCCGCCAGGTTATCGTAGGTTTTATGCAACTGATAATGGCGGCTTAAAATGTCCTGAATGGTCGAGAAAACCAGGAAGTATTCCTG‑ACGCAGGCGCAGCTCACGCCCGGAGTAGGTGGAGTCATCCGGATACAGTACGCGAGATACGTTCTCGGAGTGGTTTTTATCTTCCACTGCCGCGAAGTAGTCAc                                                                                                                       <  2:341927/251‑1 (MQ=255)                                                                                                                       catcatCTCAGGAATCGACAGTACCGGATGGGTATCATTGAGATGAATCGCGATTTTATCCGCCAGGTTATCGTAGGTTTTATGCAACTGATAATGGCGGCTTAAAATGTCCTGAATGGTCGAGAAAACCAGGAAGTATTCCTG‑ACGCAGGCGCAGCTCACGCCCGGAGTAGGTGGAGTCATCCGGATACAGTACGCGAGATACGTTCTCGGAGTGGTTTTTAGCTTCCACTGCCGCGAAGTAGTCACCCt                                                                                                                    <  1:349889/251‑1 (MQ=255)                                                                                                                             ctcAGGAATCGACAGTACCGGATGGGTATCATTGAGATGAATCGCGATTTTATCCGCCAGGTTATCGTAGGTTTTATGCAACTGATAATGGCGGCTTAAAATGTCCTGAATGGTCGAGAAAACCAGGAAGTATTCCTG‑ACGCAGGCGCAGCTCACGCCCGGAGTAGGTGGAGTCATCCGGATACAGTACGCGAGATACGTTCTCGGAGTGGTTTTTATCTTCCACTGCCGCGAAGTAGTCACCCTGGTTg                                                                                                               >  1:449881/1‑250 (MQ=255)                                                                                                                                             agcGGATGGGTATCATTGAGATGAATCGCGATTTTATCCGCCAGGTTATCGTAGGTTTTATGCAACTGATAATGGCGGCTTAAAATGGCCTGAATGGTCGAGAAAACCAGGAAGTATTCCTG‑ACGCAAGCGCAGCTCACGCCCGGAGTAGGGGGAGACATCCGGATACAGTACGCGAGATACGTTCTCGGAGTGGTTTTTATCTTCCACTGCCGCGAAGTAGTCACCCTGGTTGAATTTACCGAGGTTAAt                                                                                              <  1:10528/249‑1 (MQ=255)                                                                                                                                                ggATGGGTATCATTGAGATGAATCGCGATTTTATCCGCCAGGTTATCGTAGGTTTTATGCAACTGATAATGGCGGCTTAAAATGTCCTGAATGGTCGAGAAAACCCGGAAGTATTCCTG‑ACGCAGGCGCAGCTCACGCCCGGAGTAGGTGGAGTCATCCGGATACAGTACGCGAGATACGTTCTCGGAGTGGTTTTTATCTTCCACTGCCGCGAAGTAGTCACCCTGGTTGAATTTACCGAGGTTAATTTc                                                                                           >  2:112232/1‑251 (MQ=255)                                                                                                                                                    gggTATCATTGAGATGAATCGCGATTTTATCCGCCAGGTTATCGTAGGTTTTATGCAACTGATAATGGCGGCTTAAAATGTCCTGAATGGTCGAGAAAACCAGGAAGTATTCCTG‑ACGCAGGCGCAGCTCACGCCCGGAGTAGGTGGAGACATCCGGATACAGTACGCGCGATACGTTCTCGGAGTGGTTTTTATCTTCCACTGCCGCGAAGTAGTCACCCTGGTTGAATTTACCGAGGTTAATTTCGCt                                                                                        >  1:245786/1‑250 (MQ=255)                                                                                                                                                       tATCATTGAGATGAATCGCGATTTTATCCGCCAGGTTATCGTAGGTTTTATGCAACTGATAATGGCGGCTTAAAATGTCCTGAATGGTCGAGAAAACCAGGAAGTATTCCTG‑ACGCAGgc                                                                                                                                                                                                                       <  2:367617/120‑1 (MQ=255)                                                                                                                                                       tATCATTGAGATGAATCGCGATTTTATCCGCCAGGTTATCGTAGGTTTTATGCAACTGATAATGGCGGCTTAAAATGTCCTGAATGGTCGAGAAAACCAGGAAGTATTCCTG‑ACGCAGgc                                                                                                                                                                                                                       >  1:367617/1‑120 (MQ=255)                                                                                                                                                         tCATTGGGATGAATCGCGATTTTATCCGCCAGGTTATCGTAGGTTTTATGCAACTGATAATGGCGGCTTAAAATGTCCTGAATGGTCGAGAAAACCAGGAAGTATTCCTG‑ACGCAGGCGCAGCTCACGCCCGGAGTAGGTGGAGTCATCCGGATACAGTACGCGAGATACGTTCTCGGGGTGGTTTTTATCTTCCACTGCCGCGAAGTAGTCACCCTGGTTGAATTTACCCAGGTTAATTTCTCCACTGGc                                                                                  >  2:372192/1‑251 (MQ=255)                                                                                                                                                                 aTGAATCGCGATTTTATCCGCCAGGTTATCGTAGGTTTTATGCAACTGATAATGACGGCTTAAAATGTCCTGAATGGTCGAGAAAACCAGGAAGGATTCCTG‑ACGCAGGCGCAGCTCACGCCCGGAGTAGGTGGAGGCATCCGGATAAAGTACGCGAGCTACGTTCTCGGAGTGGTTTTTATCTTCCACTGCCGCGAAATGATCACCCTGGTTGAATTTACCGAGGCTAATTTCGCTACTGGCTTgcgccc                                                                          >  1:305157/1‑249 (MQ=255)                                                                                                                                                                 aTGAATCGCGATTTTATACGCCAGGTTATCGTAGGTTTTATGCAACTGATAATGGCGGCTTAAAATGTCCTGAATGGTCGAGAAAACCAGGAAGTATTCCTG‑ACGCAGGCGCAGCTCACGCCCGGAGTAGGTGGAGTCATCCGGATACAGTACGCGAGATACGTTCTCGGAGTGGTTTTTATCTTCCACTGCCGCGAAGTAGGCACCCTGGTTGAATTTACCTAGGTTAATTTCGCTACTGGCGTGCGCAc                                                                          <  1:69148/251‑1 (MQ=255)                                                                                                                                                                         cgATTTTATCCGCCAGGTTATCGTAGGTTTTATGCAACTGATAATGGCGGCTTAAAATGTCCTGAATGGTCGAGAAAACCAGGAAGTATTCCTG‑ACGCAGGCGCAGCTCACGCCCGGAGTAGGTGGAGTCATCCGGATACAGTACGCGAGATACGTTCTCGGAGTGGTTTTTATCTTCCACTGCCGCGAAGTAGTCACCCTGGTTGAATTTACCGAGGTTAATTTCGCTACTGGCTTGCGCACTCCACaa                                                                   >  1:456234/1‑250 (MQ=255)                                                                                                                                                                                  ccGCCAGGTTATCGTAGGTTTTATGCAACTGATAATGGCGGCTTAAAATGTCCTGAATGGTCGAGAAAACCAGGAAGTATTCCTG‑ACGCAGGCGCAGCTGACGCCCGGAGTAGGTGGAGTCATCCGGATACAGTACGCGAGATACGTTCTCGGAGTGGTTTTTATCTTCCACTGCCGCGAAGTAGTCACCCTGGTTGAATTTACCGAGGTTAATTTCGCTACTGGCTTGCGCACTCCACAAACGCAGCgtg                                                         <  2:343150/251‑1 (MQ=255)                                                                                                                                                                                   cGCCAGGGTATCGTAGGTTTTATGCAACTGATAATGGCGGCTTAAAATGTCCTGAATGGTCGAGAAAAACAGGAAGTATTCCTG‑ACGCAGGCGCAGCTCACGCCCGGAGTAGGTGGAGCCATCCGGATACAGTACGCGAGATACGTTCTCGGAGTGGGTTTTATCTTCCACTGCCGCGAAGTAGTCAACATGGTTGAATTTACCGAGGTTAATTTCGCCACTGGCTTGAGCACGCCACAAACGCAACgtgt                                                        >  1:440884/1‑251 (MQ=255)                                                                                                                                                                                        ggTTATCGTAGGTTTTATGCAACTGATAATGGCGGCTTAAAATGTCCTGAATGGTCGAGAAAACCAGGAAGTATTCCTG‑ACGCAGGCGCAGCTCACGCCCGGAGTAGGTGGAGTCATCCGGATACAGTACGCGAGATACGTTCTCGGAGTGGTTTTTATCTTCCACTGCCGCGAAGTAGTCACCCTGGTTGAATTTACCGAGGTTAATTTCGCTACTGGCTTGCGCACTCCACAAACGCAGCGTGTTGGTc                                                   <  1:372192/251‑1 (MQ=255)                                                                                                                                                                                                      ttATGCAACTGATAATGGCGGCTTAAAATGTCCTGAATGGTCGAGAAAACCAGGAAGTATTCCTG‑ACGCAGGCGCAGCTCACGCCCGGAGTAGGTGGAGTCATCCGGATACAGTACGCGAGATACGTTCTCGGAGTGGTTTTTATCTTCCACTGCCGCGAAGTAGTCACCCTGGTTGAATTTACCGAGGTTAATTTCGCTACTGGCTTGCGCACTCCACAAACGCAGCGTGTTGGt                                                    <  1:102561/236‑1 (MQ=255)                                                                                                                                                                                                      ttATGCAACTGATAATGGCGGCTTAAAATGTCCTGAATGGTCGAGAAAACCAGGAAGAATTCCTG‑ACGCAGGCGCAGCTCACGCCCGGAGTAGGTGGAGTCATCCGGATACAGTACGCGAGATACGTTCTCGGAGTGGTTTTTATCTTCCACTGCCCCGAAGAAGTAACCCTGGTTGAATTTACCGAGGTTAATTTCGCTACTGGCTTGCGCACTCCACAAACACAgtggggtttgtggaccggaaacggg                                     >  2:102561/1‑227 (MQ=255)                                                                                                                                                                                                       tATGCAACTGATAATGGCGGCTTAAAATGTCCTGAATGGTCGAGAAAACCAGGAAGTATTCCTG‑ACGCAGGCGCAGCTCACGCCCGGAGTAGGTGGAGTCATCCGGATACAGTACGCGAGATACGTTCTCGGAGTGGTTTTTATCTTCCACTGCCGCGAAGTAGTCACCCTGGTTGAATTTACCGAGGTTAATTTCGCTACTGGCTTGCGCACTCCACAAACGCAGCGTGTTGGTCGCGTCGGTGTCGTa                                     <  2:403146/250‑1 (MQ=255)                                                                                                                                                                                                                gATAATGGCGGCTTAAAATGTCCTGAATGGTCGAGAAAACCAGGAAGTATTCCTG‑ACGCAGGCGCAGCTCACGCCCGGAGTAGGTGGAGTCATCCGGATACAGTACGCGAGATACGTTCTCGGAGTGGTTTTTATCTTCCACTGCCGCGAAGTAGTCACCCTGGTTGAATTTACCGAGGTTAATGTCGCTACTGGCTTGCGCACTCCACAAACGCAGCGTGTTGGTCGCGTCGGTGTCGTAACCAGGGAt                            <  1:312388/250‑1 (MQ=255)                                                                                                                                                                                                                        cggcTTAAAATGTCCTGAATGGTCGAGAAAACCAGGAAGTATTCCTG‑ACGCAGGCGCAGCTCACGCCCGGAGTAGGTGGAGTCATCCGGATACAGTACGCGAGATACGTTCTCGGAGTGGTTTTTATCTTCCACTGCCGCGAAGTAGTCACCCTGGTTGAATTTACCGAGGTTAATTTCGCTACTGGCTTGCGCACTCCACAAACGCAGCGTGTTGGTCGCGTCGGTTTCGTAACCAGGGATTATCTGATc                   >  1:73639/1‑251 (MQ=255)                                                                                                                                                                                                                              aaaaTGTCCTGAATGGTCGAGAAAACCAGGAAGTATTCCTG‑ACGCAGGCGCAGCTCACGCCCGGAGTAGGTGGAGTCATCCGGATACAGTACGCGAGATACGTTCTCGGAGTGGTTTTTATCTTCCACTGCCGCGAAGTAGTCACCCTGGTTGAATTTACCGAGGTTAATTTCGCTACTGGCTTGCGCACTCCACAAACGCAGCGTGTTGGTCGCGTCGGTGTCGTAACCAGGGATTATCTGATCGTAAg              <  1:133602/250‑1 (MQ=255)                                                                                                                                                                                                                                   gTCCTGAATGGTCGAGAAAACCAGGAAGTATTCCTG‑ACGCAGGCGCAGCTCACGCCCGGAGTAGGTGGAGTCATCCGGATACAGTACGCGAGATACGTTCTCGGAGTGGTTTTTATCTTCCACTGCCGCGAAGTAGTCACCCTGGTTGAATTTACCGAGGTTAATTTCGCTACTGGCTTGCGCACTCCACAAACGCCGCGTGTTGGTCGCGTCGGTGTCGTAACCAGGGATTATCTGATCGTAAGCGACTc        >  2:47710/1‑251 (MQ=255)                                                                                                                                                                                                                                       tGAATGGTCGAGACAACCAGGAAGTATTACTG‑ACGCAGGCGCAGCTCACGCCCGGAGTAGGTGGCGTCATCCGGATACAGTACGCGAGCTACGTTCTCGGAGTGGTTTTTATCTTCCACTGCCGCGAAGTAGTCACCCTGGTTGAATTTACCGAGGTTAATTTCGCTACTGGCTTGCGCACTCCACCAACGCAGCGTGTTGGTCGCGTCGGTGGCGAGACACGGGATTCTCTGATCGTAAGCGACTccc      >  2:194156/1‑249 (MQ=255)                                                                                                                                                                                                                                         aaTGGTCGAGAAAACCAGGAAGTATTCCTG‑ACGCAGGCGCAGCTCACGCCCGGAGTAGGTGGAGTCATCCGGATACAGTACGCGAGATACGTTCTCGGAGTGGTTTTTATCTTCCACTGCCGCGAAGTAGTCACCCTGGTTGAATTTACCGAGTTTAATTTCGCTACTGGCTTGCGCACTCCACAAACGCAGCGTGTTGGTCGCGTCGGTGTCGTAACCAGGGATTAGCTGATCGTAAGCGACTCCCAGaa  <  2:449881/251‑1 (MQ=255)                                                                                                                                                                                                                                            ggTCGAGAAAACCAGGAAGTATTCCTG‑ACGCAGGCGCAGCTCACGCCCGGAGTAGGTGGAGTCATCCGGATACAGTACGCGAGATACGTTCTCGGAGTGGTTTTTATCTTCCACTGCCGCGAAGTAGTCACCCTGGTTGAATTTACCGAGGTTAATTTCGCTACTGGc                                                                                  >  1:398343/1‑168 (MQ=255)                                                                                                                                                                                                                                            ggTCGAGAAAACCAGGAAGTATTCCTG‑ACGCAGGCGCAGCTCACGCCCGGAGTAGGTGGAGTCATCCGGATACAGTACGCGAGATACGTTCTCGGAGTGGTTTTTATCTTCCACTGCCGCGAAGTAGTCACCCTGGTTGAATTTACCGAGGTTAATTTCGCTACTGGc                                                                                  <  2:398343/168‑1 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                  CAACCGGCCAGGTTTCCAGCGCCTCGCTCATCAGCGTGTGGTTAGTGTAGGAGAAGACCTGACAACACACCTCAAACGCGTCGTCCCAGCTAAATTGGTGCTCATCGATCAGCAGACGCATCATCTCAGGAATCGACAGTACCGGATGGGTATCATTGAGATGAATCGCGATTTTATCCGCCAGGTTATCGTAGGTTTTATGCAACTGATAATGGCGGCTTAAAATGTCCTGAATGGTCGAGGAAACCAGGAAGTATTCCTG‑ACGCAGGCGCAGCTCACGCCCGGAGTAGGTGGAGTCATCCGGATACAGTACGCGAGATACGTTCTCGGAGTGGTTTTTATCTTCCACTGCCGCGAAGTAGTCACCCTGGTTGAATTTACCGAGGTTAATTTCGCTACTGGCTTGCGCACTCCACAAACGCAGCGTGTTGGTCGCGTCGGTGTCGTAACCAGGGATTATCTGATCGTAAGCGACTCCCAGAA  >  NC_000913/3565468‑3565950

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑