Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	703,258	G→A	C118C (TGC→TGT)	nagB ←	glucosamine‑6‑phosphate deaminase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	703,258	0	G	A	100.0%	99.4 / NA	29	C118C (TGC→TGT)	nagB	glucosamine‑6‑phosphate deaminase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (10/19);  total (10/19)

CACTTCTTCGGCATCCAGCAGTGTACCAACACCGACAGTCAGGGCATATTTTGGCACCTGATTAACATCGTTATCAAAGAAACGAGAGTTTGCGACGCGAGTGTCATGAGTCAGGGTTTTGATACGAGTACGAGAAGCCAGAGAAGACGCCGGTTCGTTAAATGCAATATGACCGTCGTTACCTACACCGCCCATAAACAGATGAATTTTTCCGTAAGAACGGATTTTTTCTTCATACTGGCGGCACTCGGCGTCGATATCCGGGGCGTTGCCGTTGAGAAGGTTGATGTTTTCTGCTGGAATATCAACGTGATCGAAGAAATTACGGTGCATAAAGCTGTAGTAGCTTTCCGGATGCTCTTTCGGCAGACCGACATATTCGTCCATGTTGAAGGTGACAACGTGCTTAAAGCTGACCTGGCCTGCTTTATGCATTTCGACTAACGCTTTATAGGTGGTCATCGGCGTGCCGCCAGTCGGCAGG  >  NC_000913/703015‑703498                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                 caCTTCTTCGGCATCCAGCAGTGTACCAACACCGACAGTCAGGGCATATTTTGGCACCTGATTAACATCGTTATCAAAGAAACGAGAGTTTGCGACGCGAGTGTCATGAGTCAGGGTTTTGATACGAGTACGAGAAGCCAGAGAAGACGCCGGTTCGTTAAATGCAATATGACCGTCGTTACCTACACCGCCCATAAACAGATGAATTTTTCCGTAAGAACGGATTTTTTCTTCATACTGGCGACACTCgg                                                                                                                                                                                                                                           <  2:403631/251‑1 (MQ=255)             aTCCAGCAGTGTACCAACACCGACAGTCAGGGCATATTTTGGCACCTGATTAACATCGTTATCAAAGAAACGAGAGTTTGCGACGCGAGTGTCATGAGTCAGGGTTTTGATACGAGTACGAGAAGCCAGAGAAGACGCCGGTTCGTTAAATGCAATATGACCGTCGTTACCTACACCGCCCATAAACAGATGAATTTTTCCGTAAGAACGGATTTTTTCTTCATACTGGCGACACTCGGCGTCGATATCCg                                                                                                                                                                                                                               <  1:178771/251‑1 (MQ=255)                                                atTTTGGCACCTGATTAACATCGTTATCAAAGAAACGAGAGTTTGCGACGCGAGTGTCATGAGTCAGGGTTTTGATAAGAGTACGAGAAGCCAGAGAAGACGCCGGTTCGTTAAATGCAATATGACCGTCGTTACCTACACCGCCCATAAACAGATGAATTTTTCCGTAAGAACGGATTTTTTCTTCATACTGGCGACACTCGGCGTCGATATCCGGGGCGTTGCCGTTGAGAAGGTTGATGTTTTctgc                                                                                                                                                                                             <  1:67925/250‑1 (MQ=255)                                                         ccTGATTAACATCGTTATCAAAGAAACGAGAGTTTGCGACGCGAGTGTCATGAGTCAGGGTTTTGATACGAGTACGAGAAGCCAGAGAAGACGCCGGTTCGTTAAATGCAATATGACCGTCGTTACCTACACCGCCCATAAACAGATGAATTTTTCCGTAAGAACGGATTTTTTCTTCATACTGGCGACACTCGGCGTCGATATCCg                                                                                                                                                                                                                               <  2:309404/207‑1 (MQ=255)                                                         ccTGATTAACATCGTTATCAAAGAAACGAGAGTTTGCGACGCGAGTGTCATGAGTCAGGGTTTTGATACGAGTACGAGAAGCCAGAGAAGACGCCGGTTCGTTAAATGCAATATGACCGTCGTTACCTACACCGCCCATAAACAGATGAATTTTTCCGTAAGAACGGATTTTTTCTTCATACTGGCGACACTCGGCGTCGATATCCg                                                                                                                                                                                                                               >  1:309404/1‑207 (MQ=255)                                                                aaCATCGTTCTCAAAGAAACGAGAGTTTGCGACGCGAGTGTCATGAGTCAGGGTTTTGATACGAGTACGAGAAGCCAGAGAAGACGCCGGTTCGTTAAATGCAATATGACCGTCGTTACCTACACCGCCCATAAACAGATGAATTTTTCCGTAAGAACGGATTTTTTCTTCATACTGGCGACACTCGGCGTCGATATCCGGGGCGTTGCCGTTGAGAAGGTTGATGTTTTCTGCTGGAATATCAACg                                                                                                                                                                                >  2:418980/1‑247 (MQ=255)                                                                aaCATCGTTATCAAAGAAACGAGAGTTTGCGACGCGAGTGTCATGAGTCAGGGTTTTGATACGAGTACGAGAAGCCAGAGAAGACGCCGGTTCGTTAAATGCAATATGACCGTCGTTACCTACACCGCCCATAAACAGATGAATTTTTCCGTAAGAACGGATTTTTTCTTCATACTGGCGACACTCGGCGTCGATATCCGGGGCGTTGCCGTTGAGAAGGTTGATGTTTTCTGCTGGAATATCAACGTGa                                                                                                                                                                             <  2:30236/250‑1 (MQ=255)                                                                         aTCAAAGAAACGAGAGTTTGCGACGCGAGTGTCATGAGTCAGGGTTTTGATACGAGTACGAGAAGCCAGAGAAGACGCCGGTTCGTTAAATGCAATATGACCGTCGTTACCTACACCGCCCATAAACAGATGAATTTTTCCGTAAGAACGGATTTTTTCTTCATACTGGCGACACTCGGCGTCGATATCCGGGGCGTTGCCGTTGAGAAGGTTGATGTTTTCTGCTGGAATATCAACGTGATCGAAGAAAt                                                                                                                                                                   <  1:189608/251‑1 (MQ=255)                                                                              aGAAACGAGAGTTTGCGACGCGCGTGTCATGAGTCAGGGTTTTGATACGAGTACGAGAAGCCAGAGAAGACGCCGGTTCGTTAAATGCAATATGACCGTCGTTACCTACACCGCCCATAAACAGATGAATTTTTCCGTAAGAACGGATTTTTTCTTCATACTGGCGACACTCGGCGTCGATATCCGGGGCGTTGCCGTTGAGAAGGTTGATGTTTTCTGCTGGAATATCAACGTGATCGAAGAAATTACgg                                                                                                                                                              >  2:88641/1‑251 (MQ=255)                                                                                      gagTTTGCGACGCGAGTGTCATGAGTCAGGGTTTTGATACGAGTACGAGAAGCCAGAGAAGACGCCGGTTCGTTAAATGCAATATGACCGTCGTTACCTACACCGCCCATAAACAGATGAATTTTTCCGTAAGAACGGATTTTTTCTTCATACTGGCGACACTCGGCGTCGATATCCGGGGCGTTGCCGTTGAGAAGGTTGATGTTTTCTGCTGGAATATCAACGTGATCGAAGAAATTACGGTGCATaa                                                                                                                                                       >  1:427446/1‑250 (MQ=255)                                                                                                 gcgAGTGTCATGAGTCAGGGTTTTGATACGAGTACGAGAAGCCAGAGAAGACGCCGGTTCGTTAAATGCAATATGACCGTCGTTACCTACACCGCCCATAAACAGATGAATTTTTCCGTAAGAACGGATTTTTTCTTCATACTGGCGACACTCGGCGTCGATATCCGGGGCGTTGCCGTTGAGAAGGTTGATGTTTTCTGCTGGAATATCAACGTGATCGAAGAAATTACGGTGCATAAAGCTGTAGTAGc                                                                                                                                           >  1:127869/1‑251 (MQ=255)                                                                                                 gcgAGTGTCATGAGTCAGGGTTTTGAGACGAGTACGAGAAGCCAGAGAAGACGCCGGTTCGTTAAATGCAATATGACCGTCGTTACCTACACCGCCCATAAACAGATGAATTTTTCCGTAAGAACGGATTTTTTCTTCATACTTGCGACACTCGGCGTCGATATCCGGGGCGTTGCCGTTGAGAAGGTTGATGTTTTCTGCTGGAATATCAACGTGATCGAAGAAATTACGGTGCATAAAGCTGTAGTAGc                                                                                                                                           <  2:289383/251‑1 (MQ=255)                                                                                                     gtgtCATGAGTCAGGGTTTTGATACGAGTACGAGAAGCCAGAGAAGACGCCGGTTCGTTAAATGCAATATGACCGTCGTTACCTACACCGCCCATAAACAGATGAATTTTTCCGTAAGAACGGATTTTTTCTTCATACTGGCGACACTCGGCGTCGATATCCGGGGCGTTGCCGTTGAGAAGGTTGATGTTTTCTGCTGGAATATCAACGTGATCGAAGAAATTACGGTGCATAAAGCTGTAGTAGCTTTc                                                                                                                                       <  1:90086/251‑1 (MQ=255)                                                                                                                  gggTTTTGATACGAGTACGAGAAGCCAGAGAAGACGCCGGTTCGTTAAATGCAATATGACCGTCGTTACCTACACCGCCCATAAACAGATGAATTTTTCCGTAAGAACGGATTTTTTCTTCATACTGGCGACACTCGGCGTCGATATCCGGGGCGTTGCCGTTGAGAAGGTTGATGTTTTCTGCTGGAATATCAACGTGATCGAAGAAAGTACGGTGCATAAAGCTGTAGTAGCTTTCCGGATGCTCTTTc                                                                                                                          <  1:254436/251‑1 (MQ=255)                                                                                                                          aTACGAGTACGAGAAGCCAGAGAAGACGCCGGTTCGTTAAATGCAATATGACCGTCGTTACCTACACCGCCCATAAACAGATGAATTTTTCCGTAAGAACGGATTTTTTCTTCATACTGGCGACACTCGGCGTCGATATCCGGGGCGTTGCCGTTGAGAAGGTTGATGTTTTCTGCTGGAATATCAACGTGATCGAAGAAATTACGGTGCATAAAGCTGTAGTAGCTTTCCGGATGCTCTTTCGGCAGAcc                                                                                                                  <  2:318131/251‑1 (MQ=255)                                                                                                                           tacgagtacgagAAGCCAGAGAAGACGCCGGTTCGTTAAATGCAATATGACCGTCGTTACCTACACCGCCCATAAACAGATGAATTTTTCCGTAAGAACGGATTTTTTCTTCATACTGGCGACACTCGGCGTCGATATCCGGGGCGTTGCCGTTGAGAAGGTTGATGTTTTCTGCTGGAATATCAACGTGATCGAAGAAATTACGGTGCATAAAGCTGTAGTAGCTTTCCGGATGCTCTTTCGGCAGACCg                                                                                                                 >  1:268620/1‑251 (MQ=255)                                                                                                                                       aaGCCAGAGAAGACGCCGGTTCGTTAAATGCAATATGACCGTCGTTACCTACACCGCCCATAAACAGATGAATTTTTCCGTAAGAACGGATTTTTTCTTCATACTGGCGACACTCGGCGTCGATATCCGGGGCGTTGCCGTTGAGAAGGTTGATGTTTTCTGCTGGAATATCAACGTGATCGAAGAAATTACGGTGCATAAAGCTGTAGTAGCTTTCCGGATGCTCTTTCGGCAGACCGACATATTCGTcc                                                                                                     <  2:154821/251‑1 (MQ=255)                                                                                                                                         gCCAGAGAAGACGCCGGTTCGTTAAATGCAATATGACCGTCGTTACCTACACCGCCCATAAACAGATGAATTTTTCCGTAAGAACGGATTTTTTCTTCATACTGGCGACACTCGGCGTCGATATCCGGGGCGTTGCCGTTGAGAAGGTTGATGTTTTCTGCTGGAATATCAACGTGATCGAAGAAATTACGGTGCATAAAGCTGTAGTAGCTTTCCGGATGCTCTTTCGGCAGACCGACATATTCGTCCAt                                                                                                   <  1:549/251‑1 (MQ=255)                                                                                                                                                    cGCCGGTTCGTTAAATGCAATATGACCGTCGTTACCTACACCGCCCATAAACAGATGAATTTTTCCGTAAGAACGGATTTTTTCTTCATACTGGCGACACTCGGCGTCGATATCCGGGGCGTTGCCGTTGAGAAGGTTGATGTTTTCTGCTGGAATATCAACGTGATCGAAGAAATTACGGTGCATAAAGCTGTAGTAGCTTTCCGGATGCTCTTTCGGCAGACCGACATATTCGTCCATGTTGAAGGTGa                                                                                        <  1:407558/251‑1 (MQ=255)                                                                                                                                                             gTTAAATGCAATATGACCGTAGTTACCTACACAGCCCATAAACAGATGAATTTTTCCGTAAGAACGGATTTTTTCTTCATACTGGCGACACTCGGCGTCGATATCCGGGGCGTTGCCGTTGAGAAGGTTGATGTTTTCTGCTGGAATATCAACGTGATCGAAGAAATTACGGTGCATAAAGCTGTAGTAGCTTTCCGGATGCTCTTTCGGCAGACCGACATATTCGTCCATGTTGAAGGTGACAACGTGCt                                                                               >  1:165346/1‑251 (MQ=255)                                                                                                                                                               tAAATGCAATATGACCGTCGTTACCTACACCGCCCATAAACAGATGAATTTTTCCGTAAGAACGGATTTTTTCTTCATACTGGCGACACTCGGCGTCGATATCCGGGGCGTTGCCGTTGAGAAGGTTGATGTTTTCTGCTGGAATATCAACGTGATCGAAGAAATTACGGTGCATAAAGCTGTAGTAGCTTTCCGGATGCTCTTTCGGCAGACCGACATATTCGTCCATGTTGAAGGTGACAACGTGCtt                                                                              <  1:286233/250‑1 (MQ=255)                                                                                                                                                                aaaTGCAATATGACCGTCGTTACCTACACCGCCCATAAACAGATGAATTTTTCCGTAAGAACGGATTTTTTCTTCATACTGGCGACACTCGGCGTCGATATCCGGGGCGTTGCCGTTGAGAAGGTTGATGTTTTCTGCTGGAATATCAACGTGATCGAAGAAATTACGGTGCATAAAGCTGTAGTAGCTTTCCGGATGCTCTTTCGGCAGACCGACATATTCGTCCATGTTGAAGGTGACAACGTGCTTa                                                                             <  2:781/250‑1 (MQ=255)                                                                                                                                                                     cAATATGACCGTCGTTACCTACACCGCCCATAAACAGATGAATTTTTCCGTAAGAACGGATTTTTTCTTCATACTGGCGACACTCGGCGTCGATATCCGGGGCGTTGCCGTTGAGAAGGTTGATGTTTTCTGCTGGAATATCAACGTGATCGAAGAAATTACGGTGCATAAAGCTGTAGTAGCTTTCCGGATGCTCTTTCGGCAGACCGACATATTCGTCCATGTTGAAGGTGACAACGTGCTTAAAGCTg                                                                       <  2:365697/251‑1 (MQ=255)                                                                                                                                                                         atGACCGTCGTTACCTACACCGCCCATAAACAGCTGAATTTTTCCGTAAGAACGGATTTTTTCTTCATACTGGCGACACTCGGCGTCGATATCCGGGGCGTTGCCGTTGAGAAGGTTGATGTTTTCTGCTGGAATATCAACGTGATCGAAGAAATTACGGTGCATAAAGCTGTAGTAGCTTTCCGGATGCTCTTTCGGCAGACCGACATATTCGTCCATGTTGAAGGTGACAACGTGCTTAAAGCTGACCt                                                                   >  1:363930/1‑251 (MQ=255)                                                                                                                                                                                                                  ttCCGTAAGAACGGATTTTTTCTTCATACTGGCGACACTCGGCGTCGATATCCGGGGCGTTGCCGTTGAGAAGGTTGATGTTTTCTGCTGGAATATCAACGTGATCGAAGAAATTACGGTGCATAAAGCTGTAGTAGCTTTCCGGATGCTCTTTCGGCAGACCGACATATTCGTCCATGTTGAAGGTGACAACGTGCTTAAAGCTGACCTGGCCTGCTTTATGCATTTCGACTAACGCTTTATAggtggt                           <  2:80008/250‑1 (MQ=255)                                                                                                                                                                                                                           aaCGGATTTTTTCTTCATACTGGCGACACTCGGCGTCGATATCCGGGGCGTTGCCGTTGAGAAGGTTGATGTTTTCTGCTGGAATATCAACGTGATCGAAGAAATTACGGTGCATAAAGCTGTAGTAGCTTTCCGGATGCTCTTTCGGCAGACCGACATATTCGTCCATGTTGAAGGTGACAACGTGCTTAAAGCTGACCTGGCCTGCTTTATGCATTTCGACTAACGCTTTATAGGTGGTCATCGGCGTg                 >  2:49472/1‑251 (MQ=255)                                                                                                                                                                                                                            aCGGATTTTTTCTTCATACTGGCGACACTCGGCGTCGATATCCGGGGCGTTGCCGTTGAGAAGGTTGATGTTTTCTGCTGGAATATCAACGTGATCGAAGAAATTACGGTGCATAAAGCTGTAGTAGCTTTCCGGATGCTCTTTCGGCAGACCGACATATTCGTCCATGTTGAAGGTGACAACGTGCTTAAAGCTGACCTGGCCTGCTTTATGCATTTCGACTAACGCTTTATAGGTGGTCATCGGCGTgc                >  2:313257/1‑251 (MQ=255)                                                                                                                                                                                                                                     ttcttcATACTGGCTACACTCGGTGTCGATATCCGAGGCGTTGCCGTTGAGAAGGTTGATGATTTCTGCTGGAATAGCAACGTGATCGAAGAAATTACGGTGCATAAAGCTGTAGTAGCTTTCCGGATGCTCTTTCGGCAGACCGACATATTCGTCCATGTTGAAGGTGACAACGTGCTTAAAGCTGACCTGGCCTTCTTTCTGCATTTCGACTAACGCTTTATAGGTGGTCATCGGCGTGCCGCCAGTc        <  2:343738/250‑1 (MQ=255)                                                                                                                                                                                                                                          cATACGGGCGACACACGGCGTCGGTATCCGGGGCGTTGCCGTTGAGAAGGTTGATGTTTTCTGCTGGAATATCAAGGTGATCGAAGAAATTACGGTGCATAAAGCTGTAGTAGCTTTCCGGATGCTCTTTCGGCAGACCGACATATTCGTCCATTTTGAAGGTGACAACGGGCTTAAAGCTGGCCTGGCCTGCTTTATGCATTTCGACTAACGCTTTATAGGTGGTCATCGGCGTGCCGCCAGTCGGCAgg  <  2:379899/251‑1 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                 CACTTCTTCGGCATCCAGCAGTGTACCAACACCGACAGTCAGGGCATATTTTGGCACCTGATTAACATCGTTATCAAAGAAACGAGAGTTTGCGACGCGAGTGTCATGAGTCAGGGTTTTGATACGAGTACGAGAAGCCAGAGAAGACGCCGGTTCGTTAAATGCAATATGACCGTCGTTACCTACACCGCCCATAAACAGATGAATTTTTCCGTAAGAACGGATTTTTTCTTCATACTGGCGGCACTCGGCGTCGATATCCGGGGCGTTGCCGTTGAGAAGGTTGATGTTTTCTGCTGGAATATCAACGTGATCGAAGAAATTACGGTGCATAAAGCTGTAGTAGCTTTCCGGATGCTCTTTCGGCAGACCGACATATTCGTCCATGTTGAAGGTGACAACGTGCTTAAAGCTGACCTGGCCTGCTTTATGCATTTCGACTAACGCTTTATAGGTGGTCATCGGCGTGCCGCCAGTCGGCAGG  >  NC_000913/703015‑703498

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑