Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	821,009	G→A	D73N (GAT→AAT)	ybhM →	Bax1‑I family protein YbhM

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	821,009	0	G	A	100.0%	60.1 / NA	19	D73N (GAT→AAT)	ybhM	Bax1‑I family protein YbhM
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (10/9);  total (10/9)

ACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGGATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACTAGCCTGGGGAGCTAAGTATATCGAAATTACGGCAACCAAATATGATTCACCACCAATGTATGTCGCCATAGGGTTATTATTACTTTGTATGTATGGCTTAAGTAAGGATATCAACAAGATAAATGCCGCCATCGCGGGCGTAATATATCTGTTTTTACTCTCTTTGGTGGCGATTGTCGTTGCAAGTTTAGTTCCTGTATATGCCATTATCATCGTGTTCAGCACTGCGGGCGCGATGTTTTTAATCAGTATGCTGGCCGGTTTATTATTTAATGTTGATCCTGGTTCTCACCGTTTTATCATTATGATGACGTTGACAGGGTTGGCCCTGGTAATCATCGTG  >  NC_000913/820803‑821245                                                                                                                                                                                                               |                                                                                                                                                                                                                                             aCTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGGATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACTAGCCTGGGGAGCTAAGTATATCGAAATTACGGCAACCAAATATGATTCACCACCAATGTATGTCGCCATAGGGTTATTATTACTTTGTATGTATGGCTTAAGTAAGAATATCAACAAGATAAATGCCGCCATCGCGGGCGTAATATATCTg                                                                                                                                                                                                  <  1:230295/251‑1 (MQ=255)                aTAAAATAGATTTTCACCACGAGGGCAGTATATTAAACGGTATATGGCTCATTACCCCGTTAGTTTTGGTGGCTACGGCAGGACTAGCCTGGGGCGCTAAGTATATCGAAATTACGGCAACCAAATATGATTCACCACCAATGTATGTCGCAATAGGGGTGTTATTATTTTGTATGTATGGCTTAAGTAAGAATATGAACCAGATAAATGCCGCCATCGCGGGCGTAATATATCTGTTTTTACTCTTTTtg                                                                                                                                                                                   >  2:358333/1‑251 (MQ=255)                   aaTTAGATTTTCAGCACGAGGCCAGGATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACTAGCCTGGGGAGCTAAGTATATCGAAATTACGGCAACCAAATATGATTCACCACCAATGTATGTCGCCATAGGGTTATTATTACTTTGTATGTATGGCTTAAGTAAGAATATCAACAAGATAAATGCCGCCATCGCGGGCGTAATATATCTGTTTTTACTCTCTTtggtg                                                                                                                                                                                <  1:110525/251‑1 (MQ=255)                               aGCACGAGGCCAGGATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACTAGCCTGGGGAGCTAAGTATATCGAAATTACGGCAACCAAATATGATTCACCACCAATGTATGTCGCCATAGGGTTATTATTACTTTGTATGTATGGCTTAAGTAAGAATATCAACAAGATAAATGCCGCCATCGCGGGCGTAATATATCTGTTTTTACTCTCTTTGGTGGCGATTGTCGtt                                                                                                                                                                    <  2:11617/251‑1 (MQ=255)                                      ggCCAGGATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACTAGCCTGGGGAGCTAAGTATATCGAAATTACGGCAACCAAATATGATTCACCACCAATGTATGTCGCCATAGGGTTATTATTACTTTGTATGTATGGCTTAAGTAAGAATATCAACAAGATAAATGCCGCCATCGCGGGCGTAATATATCTGTTTTTACTCTCTTTGGTGGCGATTGTCGTTGCAAGtt                                                                                                                                                             <  1:239041/251‑1 (MQ=255)                                       cccatgATATTAAACGTTATATGGCTCATTACCTCTTTAGTTTTGGTGTCAACCGCAGGACTAGCCTGAGGAGCTAAGTTTATCGAAATTACGGCACCCAAATATTATTCACCGCCAATGTATGTCGCCATAGGGTTATTATTACTTTGTAGGTATGGCTTAAGTATGATTATCTACAAGATAATTGCCGCCATCGCGGGTGTAATATATCTGTTTTTACTCTCTTTGGTGTCGATTGTCGTTGCGAGttt                                                                                                                                                            <  2:374002/246‑1 (MQ=255)                                             atatTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACTAGCCTGGGGAGCTAAGTATATCGGAATTACGGCAACCAAATATGATTCACCACCCATGTATGTCGCCATAGGGTTATTATTAATTTGTATGTATGGCTTAAGTAAGAATATCAACAAGATAAATGCCGCCATCGGGGGAGTAATATATATGTTTTTACTCTCTTTGGTGGCGATTGTCGTTGCGAGTTTAGTTcc                                                                                                                                                      >  1:194285/1‑251 (MQ=255)                                                  aaaCGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACTAGCCTGGGGAGCTAAGTATATCGAAATTACGGCAACCAAATATGATTCACCACCAATGTATGTCGCCATAGGGTTATTATTACTTTGTATGTATGGCTTAAGTAAGAATATCAACAAGATAAATGCCGCCATCGCGGGCGTAatatat                                                                                                                                                                                                     >  1:333619/1‑199 (MQ=255)                                                  aaaCGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACTAGCCTGGGGAGCTAAGTATATCGAAATTACGGCAACCAAATATGATTCACCACCAATGTATGTCGCCATAGGGTTATTATTACTTTGTATGTATGGCTTAAGTAAGAATATCAACAAGATAAATGCCGCCATCGCGGGCGTAatatat                                                                                                                                                                                                     <  2:333619/199‑1 (MQ=255)                                                                             aGGTTTGGTGGCAACCGCAGGACTAGCCTGGGGAGCTAAGTATATCGAAATTACGGCAACCAAATATGATTCACCACCAATGTATGTCGCCATAGGGTTATTATTTCTTTGTATGTATGGCTTAAGTAAGAATATCAACAAGATAAATGCCGCCATcgc                                                                                                                                                                                                                  <  1:18794/159‑1 (MQ=255)                                                                             aGGTTTGGTGGCAACCGCAGGACTAGCCTGGGGAGCTAAGTATATCGAAATTACGGCAACCAAATATGATTCACCACCAATGTATGTCGCCATAGGGTTATTATTACTTTGTATGTATGGCTTAAGTAAGAATATCAACAAGATAAATGCCGCCATcgc                                                                                                                                                                                                                  >  2:18794/1‑159 (MQ=255)                                                                                         aaCCGCAGGACTAGCCTGGGGAGCTAAGTATATCGAAATTACGGCAACCAAATATGATTCACCACCAATGTATGTCGCCATAGGGTTATTATTACTTTGTATGTATGGCTTAAGTAAGAATATCAACAAGATAAATGCCGCCATCGCGGGCGTAATATATCTGTTTTTACTCTCTTTGGTGGCGATTGTCGTTGCAAGTTTAGTTCCTGTATATGCCATTATCATCGTGTTCAGCACTGCGGGCGCGATGt                                                                                                          >  1:398753/1‑251 (MQ=255)                                                                                           ccGCAGGTCTAGCCTGGGGAGCTAAGTATATCGAAATTACGGCAACCAAATATGATTCACCACCAATGTATGTCGCCATAGGGTTATTATTACTTTGTATGTATGGCTTAAGTAAGAATATCAACAAGATAAATGCCGCCATCGCGGGCGTAATATATCTGTTTTTACTCTCTTTGGTGGCGATTGTCTTTGCAAGTTTAGTTCCTGTATATGACATTATCATCGTGTGCAGCACTGCGGGCGCGATGttt                                                                                                        <  2:69134/251‑1 (MQ=255)                                                                                                                                                                      ccATAGGGTTATTATTACTTTGTATGTATGGCTTAAGTAAGAATATCAACAAGATAAATGCCGCCATCGCGGGCGTAATATATCTGTTTTTACTCTCTTTGGTGGCGATTGTCGTTGCAAGTTTAGTTCCTGTATATGCCATTATCATCGTGTTCAGCACTGCGGGCGCGATGTTTTTAATCAGTATGCTGGCCGGTTTATTATTTAATGTTGATCCTGGTTCTCACCGTTTTATCATTATGATGACGTTg                             >  1:28827/1‑251 (MQ=255)                                                                                                                                                                                attattaCTTTGTATGTATGGCTTAAGTAAGAATATCAACAAGATAAATGCCGCCATCGCGGGCGTAATATATCTGTTTTTACTCTCTTTGGTGGCGATTGTCGTTGCACGTTTAGTTCCTGTATATGCCATTATCATCGTGTTCAGCACTGCGGGCGCGATGTTTTTAATCAGTATGCTGGCCGGTTTATTATTTAATGTTGATCTTGTTTCTCACCGTTTTATCATTATGTTTACTTTGACAGGGtttg                   >  2:239759/1‑249 (MQ=255)                                                                                                                                                                                            tatgtatgGCTTAAGTAAGAATATCAACAAGATAAATGCCGCCATCGCGGGCGTAATATATCTGTTTTTACTCTCTTTGGTGGCGATTGTCGTTGCAAGTTTAGTTCCTGTATATGCCATTATCATCGTGTTCAGCACTGCGGGCGCGATGTTTTTAATCAGTATGCTGGCCGGTTTATTATTTAATGTTGATCCTGGTTCTCACCGTTTTATCATTATGATGACGTTGACAGGGTTGGccc                >  2:274218/1‑242 (MQ=255)                                                                                                                                                                                            tatgtatgGCTTAAGTAAGAATATCAACAAGATAAATGCCGCCATCGCGGGCGTAATATATCTGTTTTTACTCTCTTTGGTGGCGATTGTCGTTGCAAGTTTAGTTCCTGTATATGCCATTATCATCGTGTTCAGCACTGCGGGCGCGATGTTTTTAATCAGTATGCTGGCCGGTTTATTATTTAATGTTGATCCTGGTTCTCACCGTTTTATCATTATGATGACGTTGACAGGGTTGGccc                <  1:274218/242‑1 (MQ=255)                                                                                                                                                                                             atgtatgGCTTAAGTAAGAATATCAACAAGATAAATGCCGCCATCGCGGGCGTAATATATCTGTTTTTACCCTCTTTGGTGGCGATTGTCGTTGCAAGTTTAGTTCCTGTATATGCCATTATCATCGTGTTCAGCACTGCGGGCGCGATGTTTTTAATCAGTATGCTGGCCGGTTTATTATTTAATGTTGATCCTGGTTCTCACCGTTTTATCATTATGATGACGTTGACAGGGTTGGCCCTGGTAatcat      >  2:283927/1‑251 (MQ=255)                                                                                                                                                                                                  tgGCTTAAGTAAGAATATCAACAAGGTAAATGCCGCCATCGCGGGCGTAATATATCTGTTTTTAATCTCTTTGGTGGCGATTGTCGCTGCAAGTTTAGTTCCTGTATATGCCTTTATCATCGTGTTCAGCACTGCGGGCGCGATGTTTTTAATCATTATGCTGGCCGGTTTATTATTTAATGTTGATCCTGGTTCTCACCGTTTTATCATTATGATGACGTTGACAGGGTGGGCCCTGGTAATCATCGTg  >  2:365365/1‑250 (MQ=255)                                                                                                                                                                                                               |                                                                                                                                                                                                                                             ACTCGCAAAACAGCAATAAATTAGATTTTCAGCACGAGGCCAGGATATTAAACGGTATATGGCTCATTACCGCTTTAGGTTTGGTGGCAACCGCAGGACTAGCCTGGGGAGCTAAGTATATCGAAATTACGGCAACCAAATATGATTCACCACCAATGTATGTCGCCATAGGGTTATTATTACTTTGTATGTATGGCTTAAGTAAGGATATCAACAAGATAAATGCCGCCATCGCGGGCGTAATATATCTGTTTTTACTCTCTTTGGTGGCGATTGTCGTTGCAAGTTTAGTTCCTGTATATGCCATTATCATCGTGTTCAGCACTGCGGGCGCGATGTTTTTAATCAGTATGCTGGCCGGTTTATTATTTAATGTTGATCCTGGTTCTCACCGTTTTATCATTATGATGACGTTGACAGGGTTGGCCCTGGTAATCATCGTG  >  NC_000913/820803‑821245

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑