Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,385,242 G→A W468* (TGG→TGA)  yjeM → putative transporter YjeM

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,385,2420GA97.4% 129.9 / ‑5.9 39W468* (TGG→TGA) yjeMputative transporter YjeM
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base A (18/20);  total (18/21)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AGCTGACGCTGATGGCGAACGTGTCTATGACGCTTCCTTACCTGTTCCTCGCGCTGGCTTTCCCGTTCTTTAAAGCACGTCAGGATCTCGACAGACCGTTTGTGATTTTCAAAACGCATTTGTCGGCAATGATTGCGACAGTGGTTGTCGTACTGGTGGTGACATTTGCGAACGTCTTCACCATCATTCAACCTGTGGTTGAAGCCGGAGACTGGGACAGCACATTGTGGATGATTGGCGGCCCTGTCTTCTTCTCGCTGTTAGCGATGGCGATTTACCAGAACTATTGCAGCAGAGTGGCAAAAAATCCGCAGTGGGCGGTGGAATAACCCGATGCCCTATCGTCTACCTGACGATAGGGCGTAAAGACAGGAAATGTTATGGATGACACCAGCCGGGACCCGGCAATAACCGAAGATGAAATCAGGGCGCTGCAATTTTCTGCCGGGGATGTAGCGGAAATAGAGCAGACGAT  >  NC_000913/4385013‑4385487
                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                     
aGCTGACTCTGATGGCGAACGTGTCTATGACGCTTCCTTACCTGTTCCTCGCGCTGGCTTTCCCGTTCTTTAAAGCACGTCAGGATCTCGACAGACCGTTTGTGATTTTCCAAACGCATTTGTCGGCAATGATTGCGACAGTGGTTGTCGTACGGGTGGTGCCATTTGCGAACGTCTTCCCCATCATTCAACCTGAGGTTGAAGCCGGAGACTGGCACAGCACATTGCGAATGGTTGGCGGCCCTGtnttc                                                                                                                                                                                                                                  >  2:406188/1‑251 (MQ=255)
       gCTGATGGCGAACGTGTCTATGACGCTTCCTTACCTGTTCCTCGCGCTGGCTTTCCCGTTCTTTAAAGCACGTCAGGATCTCGACAGACCGTTTGTGATTTTCAAAACGCATTTGTCGGCAATGATTGCGACAGTGGTTGTCGTACTGGTGGTGACATTTGCGAACGTCTTCACCATCATTCAACCTGTGGTTGAAGCCGGAGACTGGGACAGCACATTGTGAATGATTGGCGGCCCTGTCTTCTTCTCGc                                                                                                                                                                                                                           <  2:455053/251‑1 (MQ=255)
          gATGGCGAACGTGTCTATGACGCTTCCTTACCTGTTCCTCGCGCTGGCTTTCCCGTTCTTTAAAGCACGTCAGGATCTCGACAGACCGTTTGTGATTTTCAAAACGCATTTGTCGGCAATGATTGCGACAGTGGTTGTCGTACTGGTGGTGACATTTGCGAACGTCTTCACCATCATTCAACCTGTGGTTGAAGCCGGAGACTGGGACAGCACATTGTGAATGATTGGCGGCCCTGTCTTCTTCTCGCt                                                                                                                                                                                                                          <  1:369738/249‑1 (MQ=255)
           aTGGCGAACGTGTCTATGACGCTTCCTTACCTGTTCCTCGCGCTGGCTTTCCCGTTCTTTAAAGCACGTCAGGATCTCGACAGACCGTTTGTGATTTTCAAAACGCATTTGTCGGCAATGATTGCGACAGTGGTTGTCGTACTGGTGGTGACATTTGCGAACGTCTTCACCATCATTCAACCTGTGGTTGAAGCCGGAGACTGGGACAGCACATTGTGAATGATTGGCGGCCCTGTCTTCTTCTCGCTGtt                                                                                                                                                                                                                       <  1:293380/251‑1 (MQ=255)
               cGAACTTGTCTATGCCGATTCCTGATCGGTTCCTCGCGCGGGCTTTCCCGTTCTTTAAAGCACGTCAGGATCTCGACAGACCGTTTGGGATTTTCAAAACGCATTTGTCGGCAATGATTGCGACAGTGGTTGTCGTACTGGTGGTGATATTTGCGAACGTCTTCACCATCCTTCAACCTGTGGTTGAAGCCGGAGACTGGGACAGCACATTGTGAATGATTGGCGGCCCTGTCTTCTTCTCGCTGTTAGCg                                                                                                                                                                                                                   <  2:389090/251‑1 (MQ=255)
                 aaCGTGTCTATGACGCTTCCTTACCTGTTCCTCGCGCTGGCTTTCCCGTTCTTTAAAGCACGTCAGGATCTCGACAGACCGTTTGTGATTTTCAAAACGCATTTGTCGGCAATGAGTGCGACAGTGGTTGTCGTACTGGTGGTGACATTTGCGAACGTCTTCACCATCATTCAACCTTTGGTTGAAGCCGGAGACTGGGACAGCACATTGTGAATGATTGGCGGCCCTGTCTTCTTCTCGCTGTTAGCGAt                                                                                                                                                                                                                 <  1:250274/251‑1 (MQ=255)
                   cGTGTCTATGACGCTTCCTTACCTGTTCCTCGCGCTGGCTGTCCCGTTCTTTAAAGCACGTCAGGATCTCGACAGACCGTTTGTGATTTTCAAATCGCATTTGTCGGCAAGGATTGCGACAGTGGTTGTCGTACTGGTGGTGACATTTGCGAACGTCTTCACCATCATTCAACCTGTGGTTGAAGCCGGAGACTGGGACAGCACAGTGTGGATGATTGGCGGCCCTGTCTTCTTCTCTCTGTTAGCGATgg                                                                                                                                                                                                               <  2:298261/251‑1 (MQ=255)
                            gACGCTTCCTTACCTGTTCCTCGCGCTGGCTTTCCCGTTCTTTATAGCACGTCAGGATCTCGTCAGACCGTTTGTGATTTTCAAAACGCATTTGTCGGCAATGATTGCGACAGTGGTTGTCGTACTGGTGGTGTCATTTGCGAACGTCTTCACCATCATTCAACCTGTGGTTGAAGCCGGATACTGGGCCAGCACATTGTGAATGATTGGCGGCCCTGTCTTCTTCTCGCTTTTAGCGATGGCGAGTTAcc                                                                                                                                                                                                      <  1:210959/251‑1 (MQ=255)
                                  tCCTTACCTGTTCCTCGCGCTGGCTTTCCCGTTCTTTAAAGCACGTCAGGATCTCGACAGACCGTTTGTGATTTTCAAAACGCATTTGTCGGCAATGATTGCGACAGTGGTTGTCGTACTGGTGGTGACATTTGCGAACGTCTTCACCATCATTCAACCTGTGGTTGAAGCCGGAGACTGGGACAGCACATTGTGAATGATTGGCGGCCCTGTCTTCTTCTCGCTGTTAGCGATGGCGATTTACCAGAACt                                                                                                                                                                                                >  2:163172/1‑251 (MQ=255)
                                   ccTTACCTGTTCCTCGCGCTGGCTTTCCCGTTCTTTAAAGCACGTCAGGATCTCGACAGACCGTTTGTGATTTTCAAAACGCATTTGTCGGCAATGATTGCGACAGTGGTTGTCGTACTGGTGGTGACATTTGCGAACGTCTTCACCATCATTCAACCTGTGGTTGAAGCCGGAGACTGGGACAGCACATTGTGAATGATTGGCGGCCCTGTCTTCTTCGCGCTGTTAGCGATGGCGATTTACCAGAACTa                                                                                                                                                                                               <  1:330239/251‑1 (MQ=255)
                                                    gcTGGCTTTCCCGTTCTTTAAAGCACGTCAGGCTCTCGACAGACCGTTTGTGATTTTCAAAACGCATTTGTCGGCAATGATTGCGACAGTGGTTGTCGTACTGGTGGTGACATTTGCGAACGTCTTCACCATCATTCAACCTGTGGTTGAAGCCGGAGACTGGGACAGCACATTGTGAATGATTGGCGGCCCTGTCTTCTTCTCGCTGTTAGCGATGGCGATTTACCAGAACTATTGCAGCAGAGTGGCa                                                                                                                                                                               >  1:386102/1‑250 (MQ=255)
                                                            tCCCGTTCTTTAAAGCACGTCAGGATCTCGACAGACCGTTTGTGATTTTCAAAACGCATTTGTCGGCAATGATTGCGACAGTGGTTGTCGTACTGGTGGTGACATTTGCGAACGTCTTCACCATCATTCAACCTGTGGTTGAAGCCGGAGACGGGGACAGCACATTGTGAATGATTGGCGGCCCTGTCTTCTTCTCGCTGTTAGCGATGGCGATTTACAAGAACTATTGCATAAGAGTGGCAAAAAATCCg                                                                                                                                                                      >  2:318516/1‑251 (MQ=255)
                                                               cGTTCTTTAAAGCACGTCAGGATCTCGACAGACCGTTTGTGATTTTCAAAACGCATTTGTCGGCAATGATTGCGACAGTGGTTGTCGTACTGGTGGTGACATTTGCGAACGTCTTCACCATCATTCAACCTGTGGTTGAAGCCGGAGACTGGGACAGCACATTGTGAATGATTGGCGGCCCTGTCTTCTTCTCGCTGTTAGCGATGGCGATTTACCAGAACTATTGCAGCAGAGTGGCAAAAAATCCGc                                                                                                                                                                     >  1:5447/1‑249 (MQ=255)
                                                                  tCTTTAAAGCACGTCAGGATCTCGACAGACCGTTTGTGATTTTCAAAACGCATTTGTCGGCAATGATTGCGACAGTGGTTGTCGTACTGGTGGTGACATTTGCGAACGTCTTCACCATCATTCAACCTGTGGTTGAAGCCGGAGACTGGGACAGCACATTGTGAATGATTGGCGGCCCTGTCTTCTTCTCGCTGTTAGCGATGGCGATTTACCAGAACTATTGCAGCAGAGTGGCAAAAAATCCGCAGTg                                                                                                                                                                 <  1:206915/250‑1 (MQ=255)
                                                                             cGTCAGGATCTCGACAGACCGTTTGTGATTTTCAAAACGCATTTGTCGGCAATGATTGCGACAGTGGTTGTCGTACTGGTGGTGACATTTGCGAACGTCTTCACCATCATTCAACCTGTGGTTGAAGCCGGAGACTGGGACAGCACATTGTGAATGATTGGCGGCCCTGTCTTCTTCTCGCTGTTAGCGATGGCGATTTACCAGAACTATTGCAGCAGAGTGGCAAAAAATCCGCAGTGGGCGGTGGAATa                                                                                                                                                     <  2:38590/251‑1 (MQ=255)
                                                                                  ggATCTCGACAGACCGTTTGTGATTTTCAAAACGCATTTGTCGGCAATGATTGCGACAGTGGTTGTCGTACTGGTGGTGACATTTGCGAACGTCTTCACCATCATTCAACCTGTGGTTGCAGCCGGAGACTGGGACAGCACATTGTGAATGATTGGCGGCCATGTCTTCTTCTCGCTGTTAGCGATGGCGATTTACCAGAACTATTGCAGCAGAGTGGCAAAAAATCCGCAGCGGGAGGTGGAATAACCCg                                                                                                                                                >  1:453954/1‑251 (MQ=255)
                                                                                  ggATCTCGACAGACCGTTTGTGATTTTCAAAACGCATTTGTCGGCAATGATTGCGACAGTGGTTGTCGTACTGGTGGTGACATTTGCGAACGTCTTCACCATCATTCAACCTGTGGTTGAAGCCGGAGACTGGGACAGCACATTGTGAATGATTGGCGGCCCTGTCTTCTTCTCGCTGTTAGCGATGGCGATTTACCAGAACTATTGCAGCAGAGTGGCAAAAAATCCGCAGTGGGCGGTGGAATAACCCg                                                                                                                                                <  1:122236/251‑1 (MQ=255)
                                                                                            aGACCTTTTGTGATGTGCAAAACGCATTTGTCGGCAATGATTGCGACAGTGTTTGTCGTCCTGGTGGTGACATTTGCTAACGTCTTTACCATCATTCAACCTGTTGTTGAAGCCGGAGACTGGGACAGCACATTGTGAATGATTGGCGGCCCTGTCTTCTTCTCGCTGTTAGCTATGGCGATTTACCAGAACTATTGCAGCAGAGTTGCAAAAAATCCGCAGTGCGCGGTGGAATAACCCGATGCCCTATc                                                                                                                                      <  1:422681/251‑1 (MQ=255)
                                                                                                cGTTTGTGATTTTCAAAACGCATTTGTCGGCAATGATTGCGACAGTGGTTGTCGTACTGGTGGTGACATTTGCGAACGTCTTCACCATCATTCAACCTGTGGTTGAAGCCGGAGACTGGGACAGCACATTGTGAATGATTGGCGGCCCTGTCTTCTTCTCGCTGTTAGCGATGGCGATTTACCAGAACTATTGCAGCAGAGTGGCAAAAAATCCGCAGTGGGCGGTGGAATAACCCGATGCCCTATCGTCt                                                                                                                                  >  1:289222/1‑251 (MQ=255)
                                                                                                        aTTTTCAAAACGCATTTGTCGGCAATGATTGCGACAGTGGTTGTCGTACTGGTGGTGACATTTGCGAACGTCTTCACCATCATTCAACCTGTGGTTGAAGCCGGAGACTGGGACAGCACATTGTGAATGATTGGCGGCCCTGTCTTCTTCTCGCTGTTAGCGATGGCGATTTACCAGAACTATTGCAGCAGAGTGGCAAAAAATCCGCAGTGGGCGGTGGAATAACCCGATGCCCTATCGTCTACCTGACg                                                                                                                          <  1:77759/251‑1 (MQ=255)
                                                                                                             cAAAACGCATTTGTCGGCAATGATTGCGACAGTGGTTGTCGTACTGGTGGTGACATTTGCGAACGTCTTCACCATCATTCAACCTGTGGTTGAAGCCGGAGACTGGGACAGCACATTGTGAATGATTGGCGGCCCTGTCTTCTTCTCGCTGTTAGCGATGGCGATTTACCAGAACTATTGCAGCAGAGTGGCAAAAAATCCGCAGTGGGCGGTGGAATAACCCGATGCCCTATCGTCTACCTGACGATAgg                                                                                                                     >  1:362485/1‑251 (MQ=255)
                                                                                                                aaCGCATTTGTCGGCAATGATTGCGACAGTGGTTGTCGTACTGGTGGTGACATTTGCGAACGTCTTCACCATCATTCAACCTGTGGTTGAAGCCGGAGACTGGGACAGCACATTGTGAATGATTGGCGGCCCTGTCTTCTTCTCGCTGTTAGCGATGGCGATTTACCAGAACTATTGCAGCAGAGTGGCAAAAAATCCGCAGTGGGCGGTGGAATAACCCGATGCCCTATCGTCTACCTGACGATAGGGCg                                                                                                                  >  2:64240/1‑251 (MQ=255)
                                                                                                                      tttGTCGGCAATGATTGCGACAGTGGTTGTCGTACTGGTGGTGACATTTGCGAACGTCTTCACCATCATTCAACCTGTGGTTGAAGCCGGAGACTGGGACAGCACATTGTGAATGATTGGCGGCCCTGTCTTCTTCTCGCTGTTAGCGATGGCGATTTACCAGAACTATTGCAGCAGAGTGGCAAAAAATCCGCAGTGGGCGGTGGAATAACCCGATGCCCTATCGTCTACCTGACGATAGGGCGTaa                                                                                                               >  1:96622/1‑248 (MQ=255)
                                                                                                                       ttGTCGGCAATGATTGCGACAGTGGTTGTCGTACTGGTGGTGACATTTGCGAACGTCTTCACCATCATTCAACCTGTGGTTGAAGCCGGAGACTGGGACAGCACATTGTGAATGATTGGCGGCCCTGTCTTCTTCTCGCTGTTAGCGATGGCGATTTACCAGAACTATTGCAGCAGAGTGGCAAAAAATCCGCAGTGGGCGGTGGAATAACCCGATGCCCTATCGTCTACCTGACGATAGGGCGTAAAGa                                                                                                            <  1:425801/250‑1 (MQ=255)
                                                                                                                              cAATGATTGCGACAGTGGTTGTCGTACTGGTGGTGACATTTGCGAACGTCTTCACCATCATTCAACCTGTGGTTGAAGACGGAGACTGGGACAGCACATTGTGAATGATTGGCGGCCCTGTCTTCTTCTCGCTGTTAGCGATGGCGATTTACCAGCACTATTGCAGCAGAGTGGCAAAAAATCCGCAGTGGGCGGTGGAATAACCCGATGCCCTATCGTCTACCTGACGATAGGGCGTACAGACAGGAAAt                                                                                                    >  2:295736/1‑251 (MQ=255)
                                                                                                                                    ttGCGACAGTGGTTGTCGTACTGGTGGTGACATTTGCGAACGTCTTCACCATCATTCAACCTGTGGTTGAAGCCGGAGACTGGGACAGCACATTGTGAATGATTGGCGGCCCTGTCTTCTTCTCGCTGTTAGCGATGGCGATTTACCAGAACTATTGCAGCAGAGTGGCAAAAAATCCGCAGTGGGCGGTGGAATAACCCGATGCCCTATCGTCTACCTGACGATAGGGCGTAAAGACAGGAAATGTTATg                                                                                              >  1:64167/1‑251 (MQ=255)
                                                                                                                                     tGCGACAGTGGTTGTCGTACTGGTGGTGACATTTGCGAACGTCTTCACCATCATTCAACCTGTGGTTGAAGCCGGAGACTGGGACAGCACATTGTGAATGATTGGCGGCCCTGTCTTCTTCTCGCTGTTAGCGATGGCGATTTACCAGAACTATTGCAGCAGAGTGGCAAAAAATCCGCAGTGGGCGGTGGAATAACCCGATGCCCTATCGTCTACCTGACGATAGGGCGTAAAGACAGGAAATGTTATgg                                                                                             >  1:92684/1‑251 (MQ=255)
                                                                                                                                      gCGACAGTGGTTGTCGTACTGGTGGTGACGTTTGCGAACGCCTTCACCATCATTCAACCTGTGGTTGAAGCCGGAGACTGGGACAGCACATTGTGAATGATTGGCGGCCCTGTCTTCTTCTCGCTGTTAGCGATGGCGATTTACCAGAACTATTGCAGCAGAGTGGCAAAAAATCCGCAGTGGGCGGTGGAATAACCCGATGCCCTATCGTCTACCTGACGATAGGGCGTAAAGACAGGAAATGTTATgg                                                                                             <  1:128849/250‑1 (MQ=255)
                                                                                                                                         aCAGTGGTTGTCGTACTGGTGGTGACATTTGCGAACGTCTTCACCATCATTCAACCTGTGGTTGAAGCCGGAGACTGGGACAGCACATTGTGAATGATTGGCGGCCCTGTCTTCTTCTCGCTGTTAGCGATGGCGATTTACCAGAACTATTGCAGCAGAGTGGCAAAAAATCCGCAGTGGGCGGTGGAATAACCCGATGCCCTATCGTCTACCTGACGATAGGGCGTAAAGACAGGAAATGTTATGGATGa                                                                                         <  2:5056/251‑1 (MQ=255)
                                                                                                                                         aCAGTGGATGTCGTACTGGTGGAGACATTTGCGAACGTCTTCACCATCATTCAACCTGTGATTGAAGCCGGAGACTGGGACAGCACATTGTGAATGATTGGCGGCCCTGTCTTCTTCTCGCTGTTAGCGATGGCGATTTACCAGAACTATTGCAGCAGAGTGGCAAAAAATCCGCAGTGGGCGGTGGAATAAACCGATGACCTATCGTCTACCTGACGATAGGCCGTACAGACAGGAAATGTTATGGATg                                                                                          >  2:234891/1‑250 (MQ=255)
                                                                                                                                              ggTTGTCGTACTGGTGGTGACATTTGCGAACGTCTTCACCATCATTCAACCTGTGGTTGAAGCCGGAGACTGGGACAGCACATTGTGAATGATTGGCGGCCCTGTCTTCTTCTCGCTGTTAGCGATGGCGATTTACCAGAACTATTGCAGCAGAGTGGCAAAAAATCCGCAGTGGGCGGTGGAATAACCCGATGCCCTATCGTCTACCTGACGATAGGGCGTAAAGACAGGAAATGTTATGGATGACACCa                                                                                    <  2:64167/251‑1 (MQ=255)
                                                                                                                                                  gTCGTACTGGTGGTGACATTTGCGAACGTCTTCACCATCATTCAACCTGTGGTTGAAGCCGGAGACTGGGACAGCACATTGTGAATGATTGGCGGCCCTGTCTTCTTCTCGCTGTTAGCGATGGCGATTTACCAGAACTATTGCAGCAGAGTGGCAAAAAATCCGCAGTGGGCGGTGGAATAACCCGATGCCCTATCGTCTACCTGACGATAGGGCGTAAAGACAGGAAATGTTATGGATGACACCAGCCg                                                                                >  1:61663/1‑251 (MQ=255)
                                                                                                                                                                 aCATTTGCGAACGTCTTCACCATCATTCAACCTGTGGTTGAAGCCGGAGACTGGGACAGCACATTGTGAATGATTGGCGGCCCTGTCTTCTTCTCGCTGTTAGCGATGGCGATTTACCAGAACTATTGCAGCAGAGTGGCAAAAAATCCGCAGTGGGCGGTGGAATAACCCGATGCCCTATCGTCTACCTGACGATAGGGCGTAAAGACAGGAAATGTTATGGATGACACCAGCCGGGACCCGGCAATAAc                                                                 <  1:76941/251‑1 (MQ=255)
                                                                                                                                                                  cATTTGCGCACGTCTTCACCATCATTCAACCTGTGGTTGAAGCCGGAGACTGGGACCGCACATTGTGAATGATTGGCGGCCCTGTCTTCTTCTCGCTGTTAGCGATGGCGATTTACCAGAACTATTGCAGCAGAGTGGCAAAAAATCCGCAGTGGGCGGTGGAATAACCCGATGCCCTATCGTCTACCTGACGATAGGGCGTAAAGACAGGAAATGTTATGGATGACACCAGCCGGGACCCGGCAATAAcc                                                                >  2:434491/1‑251 (MQ=255)
                                                                                                                                                                         gAACGTCTTCACCATCATTCAACCTGTGGTTGACGCCGGAGACTGGGACAGCACATTGTGAATGATTGGCGGCCCTGTCTTCTTCTCGCTGTTAGCGATGGCGATTTACCAGAACTATTGCAGCAGAGTGGCAAAAAATCCGCAGTGGGCGGTGGAATAACCCGATGCCCTATCGTCTACCTGACGATAGGGCGTAAAGACAGGAAATGTTATGGATGACACCAGCCGGGACCCGGCAATAACCGAAGATg                                                         >  1:145419/1‑251 (MQ=255)
                                                                                                                                                                           aCGTCTTCACCATCATTCAACCTGTGGTTGAAGCCGGAGACTGGGACAGCACATTGTGAATGATTGGCGGCCCTGTCTTCTTCTCGCTGTTAGCGATGGCGATTTACCAGAACTATTGCAGCAGAGTGGCAAAAAATCCGCAGTGGGCGGTGGAATAACCCGATGCCCTATCGTCTACCTGACGATAGGGCGTAAAGACAGGAAATGTTATGGATGACACCAGCCGGGACCCGGCAATAACCGAAGATGa                                                        >  2:29040/1‑250 (MQ=255)
                                                                                                                                                                                            cAACCTGTGTTTGAAGCCGGAGACTGGGACAGCACATTGTGAATGATTGGCGGCCCTGTCTTCTTCTCGCTGTTAGCGATTGCGATTTACCAGAACTATTGCAGCAGAGTGGCAAAAAATCCTCAGTGGTAGGTGGAATAACCCTATGCCCTATCGTCTACCTGACGATATGGCGTAAAGACAGGAAATGTTATGGATGGCACCAGCCGGGACCCGGCAATAACCGAAGATGAAATCAGGGCGCTGCAAt                                       <  1:275312/250‑1 (MQ=255)
                                                                                                                                                                                                                  aCTGGGACAGCACATTGTGAATGATTGGCGGCCCTGTCTTCTTCTCGCTGTTAGCGATGGCGATTTACCAGAACTATTGCAGCAGAGTGGCAAAAAATCCGCAGTGGGCGGTGGAATAACCCGATGCCCTATCGTCTACCTGACGATAGGGCGTAAAGACAGGAAATGTTATGGATGACACCAGCCGGGACCCGGCAATAACCGAAGATGAAATCAGGGCGCTGCAATTTTCTGCCGGGGATGTAGCGGaa                <  1:14293/251‑1 (MQ=255)
                                                                                                                                                                                                                                 tgtgAATGATTGGCGGCCCTGTCTTCTTCTCTCTGTTAGGTATGGCGATTTACCAGAACTATTGCAGCAGAGTGGCAAAAAATCCGCAGTGGGCGGTGGAATAACCCGATGCCCTATCGTTTACCTGACGATAGGGCGTAAAGACAGGAAATGTTATGGATTGCGCCAGCCGGGACCCGGCAATAACCGAAGATGAAATCAGGGCGCTGCAATTTTCTGCCGGGGATGTAGCGGAAATAGAGCAGACGAt  <  2:370729/250‑1 (MQ=255)
                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                     
AGCTGACGCTGATGGCGAACGTGTCTATGACGCTTCCTTACCTGTTCCTCGCGCTGGCTTTCCCGTTCTTTAAAGCACGTCAGGATCTCGACAGACCGTTTGTGATTTTCAAAACGCATTTGTCGGCAATGATTGCGACAGTGGTTGTCGTACTGGTGGTGACATTTGCGAACGTCTTCACCATCATTCAACCTGTGGTTGAAGCCGGAGACTGGGACAGCACATTGTGGATGATTGGCGGCCCTGTCTTCTTCTCGCTGTTAGCGATGGCGATTTACCAGAACTATTGCAGCAGAGTGGCAAAAAATCCGCAGTGGGCGGTGGAATAACCCGATGCCCTATCGTCTACCTGACGATAGGGCGTAAAGACAGGAAATGTTATGGATGACACCAGCCGGGACCCGGCAATAACCGAAGATGAAATCAGGGCGCTGCAATTTTCTGCCGGGGATGTAGCGGAAATAGAGCAGACGAT  >  NC_000913/4385013‑4385487

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑