Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,380,268	C→T	D84N (GAT→AAT)	frdB ←	fumarate reductase iron‑sulfur protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,380,268	0	C	T	100.0%	198.8 / NA	58	D84N (GAT→AAT)	frdB	fumarate reductase iron‑sulfur protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (30/28);  total (30/28)

AGGCCAAACTGCGGGCACGCGGCGTAGCACAAACCACAGTTGATGCAACCGGAGAACTGGTGATACTTCGCCATCTGCGCCGGGGTCTGGATGTTAGTACCCTGATCCGCGGTGCGGGAGTTGCCGATGATGTACGGTTTGATCGCTTCCAGACTTTCGATGAAGTGGGTCATATCGACCACCAGATCGCGTTCAATCGGGAAGTTAGCTAACGCTTCAACCTTCATACCGTCGGTGTAATCACGCAGGAAGGTTTTACATGCCAGTTTTGGCACGTTGTTAACCATCATGCCGCAGGAACCACAAATCGCCATACGGCAGGACCAGCGGTAGCTCAGGTCCGGTGCCAGGTTGTCTTTGATGTAGCCCAGCGCATCCAGTAATGAGGTAGTTGCGTCATAAGGCACTTCATAGAATGCGCTATGCGGTGCGGTATCGACTTCCGGGTTATAGCGCACCACCTCAATTTTCAGGTTTTTCATCTC  >  NC_000913/4380027‑4380511                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                    aGGCCAAACTGCGGGCACGCGGCGTAGCACAAACCACAGTTGATGCAACCGGAGAACTGGTGATACTTCGCCATCTGCGCCGGGGTCTGGATGTTAGTACCCTGATCCGCGGTGCGGGAGTTGCCGATGATGTACGGTTTGATCGCTTCCAGACTTTCGATGAAGTGGGTCATATCGACCACCAGATCGCGTTCAATCGGGAAGTTAGCTAACGCTTCAACCTTCATACCGTCGGTGTAATTACGCAGGaa                                                                                                                                                                                                                                            <  1:314239/251‑1 (MQ=255)   gCCAAACTGCGGGCACGCGGCGTAGCACAAACCACAGTTGATGCAACCGGAGAACTGGTGATACTTCGCCATCTGCGCCGGGGTCTGGATGTTAGTACCCTGATCCGCGGTGCGGGAGTTGCCGATGATGTACGGTTTGATCGCTTCCAGACTTTCGCTGCAGTGGGTCATATCGACCACCAGATCGCGTTCAATCGGGAAGTTAGCTAACGCTTCAACCTTCATACCGTCGGTGTAATTACGCAGGacgg                                                                                                                                                                                                                                          >  2:336892/1‑248 (MQ=255)                 aCGCGGCGTAGCACAAACCACAGTTGATGCAACCGGAGAACTGGTGATACTTCGCCATCTGCGCCGGGGTCTGGATGTTAGTACCCTGATCCGCGGTGCGGGAGTTGCCGATGATGTACGGTTTGATCGCTTCCAGACTTTCGATGAAGTGGGTCATATCGACCACCAGATCGCGTTCAATCGGGAAGTTAGCTAACGCTTCAACCTTCATACCGTCGGTGTAATTACGAAGGAAGGTTTTACATGCAAGt                                                                                                                                                                                                                            >  1:404085/1‑251 (MQ=255)                           gCACAAACCACAGTTGATGCAACCGGAGAACTGGTGATACTTCGCCATCTGCGCCGGGGTCTGGATGTTAGTACCCTGATCCGCGGTGCGGGAGTTGCCGATGATGTACGGTTTGATCGCTTCCAGACTTTCGATGAAGTGGGTCATATCGACCACCAGATCGCGTTCAATCGGGAAGTTAGCTAACGCTTCAACCTTCATACCGTCGGTGTAATTACGCAGGAAGGTTTTACATGCCAGTTTTGGCACgt                                                                                                                                                                                                                  >  2:315567/1‑251 (MQ=255)                                         tGATGCAACCGGAGAACTGGTGATACTTCGCCATCTGCGCCGGGGTCTGGATGTTAGTACCCTGATCCGCGGTGCGGGAGTTGCCGATGATGTACGGTTTGATCGCTTCCAGACTTTCGATGAAGTGGGTCATATCGACCACCAGATCGCGTTCAATCGGGAAGTTAGCTAACGCTTCAACCTTCATACCGTCGGTGTAATTACGCAGGAAGGTTTTACATGCCAGTTTTGGCACGTTGTTAACCATCATg                                                                                                                                                                                                    >  1:373828/1‑251 (MQ=255)                                              cAACCGGAGAACTGGTGATACTTCGCCATCTGCGCCGGGGTCTGGATGTTAGTACCCTGATCCGCGGTGCGGGAGTTGCCGATGATGTACGGTTTGATCGCTTCCAGACTTTCGATGAAGTGGGTCATATCGACCACCAGATCGCGTTCAATCGGGAAGTTAGCTAACGCTTCAACCTTCATACCGTCGGTGTAATTACGCAGGAAGGTTTTACATGCCAGTTTTGGCACGTTGTTAACCATCATGCCGc                                                                                                                                                                                                >  1:422103/1‑250 (MQ=255)                                               aaCCGGAGAACTGGTGAAACAGGGCGTTCAGCTCCGGGGTCTGGATGTTAGTACCCTGATCCTCGGTGCGGGAGTTGCCGATGATGTACGGTTTGATCGCTTCCAGACTGTGTATGAAGTCGGTCATATCGACCACCAGATCGCTTTCAATCGGGAAGTTAGCTAACGCTTCAACCTTCATACCGTCGGTCTAATTACGCAGGAAGTTTGTACATGCCAGTTTTGGCACGTTGTTAAccttgacgtcgcag                                                                                                                                                                                              <  2:458552/251‑13 (MQ=255)                                                  cGGAGAACTGGTGATACTTCGCCATCTGCGCCGGGGTCTGGATGTTAGTACCCTGATCCGCGGTGCGGGAGTTGCCGATGATGTACGGTTTGATCGCTTCCAGACTTTCGATGAAGTGGGTCATATCGACCACCAGATCGCGTTCAATCGGGAAGTTAGCTAACGCTTCAACCTTCATACCGTCGGTGTAATTACGCAGGAAGGTTTTACATGCCAGTTTTGGCACGTTGTTAACCATCATGCCGCAGGa                                                                                                                                                                                            >  1:196222/1‑250 (MQ=255)                                                    gagaACTGGTGATACTTCGCCATCTGCGCCGGGGTCTGGATGTTAGTACCCTGATCCGCGGTGCGGGAGTTGCCGATGATGTACGGTTTGATCGCTTCCAGACTTTCGATGAAGTGGGTCATATCGACCACCAGATCGCGTTCAATCGGGAAGTTAGCTAACGCTTCAACCTTCATACCGTCGGTGTAATTACGCAGGAAGGTTTTACATGCCAGTTTTGGCACGTTGTTAACCATCATGCCGCAGGAAcc                                                                                                                                                                                         <  2:285530/251‑1 (MQ=255)                                                      gaACTGGTGATACTTCGCCATCTGCGCCGGGGTCTGGATGTTAGTACCCTGATCCGCGGTGCGGGAGTTGCCGATGATGTACGGTTTGATCGCTTCCAGACTTTCGATGAAGTGGGTCATATCGACCACCAGATCGCGTTCAATCGGGAAGTTAGCTAACGCTTCAACCTTCATACCGTCGGTGTAATTACGCAGGAAGGTTTTACATGCCAGTTTTGGCACGTTGTTAACCATCATGCCGCAGGAACcac                                                                                                                                                                                       >  1:371683/1‑251 (MQ=255)                                                        aCTGGTGATACTTCGCCATCTGGGCCGGGGTCTGGATGTTAGTACCCTGATCCGCGGTGCGGGAGTTGCCGATGATGTACGGTTTGATCGCTTCCAGACTTTCGATGAAGTGGGTCATATCGACCACCAGATCGCGTTCAATCGGGAAGTTAGCTAACGCTTCAACCTTCATACCGTCGGTGTAATTACGCAGGAAGGTTTTACATGCCAGTTTTGGCACGTTGTTAACCATCATGCCGCAGGAACCACaa                                                                                                                                                                                     <  2:330483/251‑1 (MQ=255)                                                             tGATACTTCGCCATCTGCGCCGGGGTCTGGATGTTAGTACCCTGATCCGCGGTGCGGGAGTTGCCGATGATGTACGGTTTGATCGCTTCCAGACTTTCGATGAAGTGGGTCATATCGACCACCAGATCGCGTTCAATCGGGAAGTTAGCTAACGCTTCAACCTTCATACCGTCGGTGTAATTACGCAGGAAGGTTTTACATGCCAGTTTTGGCACGTTGTTAACCATCATGCCGCAGGAACCACAAATCGc                                                                                                                                                                                >  2:165824/1‑251 (MQ=255)                                                               aTACTTCGCCATCTGCGCCGGGGTCTGGATGTTAGTTCCCTGATCCGCGGTTGGGGAGTTGCCGATGATGTACGGTTTGATCGCTTCCAGACTTTCGATGAAGTGGGTCATATCGACCACCAGATCGCGTTCAATCGGGAAGTTAGCTAACGCTTCAACCTTCATACCGTCGGTGTAATTACGCAGGAAGGTTTTACATGCCAGTTTTGGCACGTTGTTAACCATCATGCCGCAGGAACCACAAATCGCCa                                                                                                                                                                              <  2:1125/251‑1 (MQ=255)                                                                             gcgcCGGGGTCTGGATGTTAGTACCCTGATCCGCGGTGCGGGAGTTGCTGATGATGTACGGTTTGATCGCTTCCAGACTTTCGATGAAGTGGGTCATATCGACCACCAGATCGCGTTCAATCGGGAAGTTAGCTAACGCTTCAACCTTCATACCGTCGGTGTAATTACGCAGGAATGTTTTACATGCCAGTTTGGGCTCGTTGTTAACCATCATGCCGCAGGAACCACAAATCGCCATACGGCAGGAcc                                                                                                                                                                  <  1:181491/249‑1 (MQ=255)                                                                             gcgcCGGGGTCTGGATGTTAGTACCCTGATCCGCGGTGCGGGAGTTGCCGATGATGTACGGTTTGATCGCTTCCAGACTTTCGATGAAGTGGGTCATATCGACCACCAGATCGCGTTCAATCGGGAAGTTAGCTAACGCTTCAACCTTCATACCGTCGGTGTAATTACGCAGGAAGGTTTTACATGCCAGTTTTGGCACGTTGTTAACCATCATGCCGCAGGAACCACAAATCGCCATACGGCAGGAc                                                                                                                                                                   <  1:118074/248‑1 (MQ=255)                                                                                    ggTCTGGATGTTAGTACCCTGATCCGCGGTGCGGGAGTTGCCGATGATGTACGGTTTGATCGCTTCCAGACTTTCGATGAAGTGGGTCATATCGACCACCAGATCGCGTTCAATCGGGAAGTTAGCTAACGCTTCAACCTTCATACCGTCGGTGTAATTACGCAGGAAGGTTTTACATGCCAGTTTTGGCACGTTGTTAACCATCATGCCGCAGGAACCACAAATCGCCATACGGCAGGACCAGCGGTAGc                                                                                                                                                         <  1:200619/251‑1 (MQ=255)                                                                                       cTGGATGTTAGTACCCTGATCCGCGGTGCGGGAGTTGCCGATGATGTACGGTTTGATCGCTTCCAGACTTTCGATGAAGTGGGTCATATCGACCACCAGATCGCGTTCAATCGGGAAGTTAGCTAACGCTTCAACCTTCATACCGTCGGTGTAATTACGCAGGAAGGTTTTACATGCCAGTTTTGGCACGTTGTTAACCATCATGCCGCAGGAACCACAAATCGCCATACGGCAGGACCAGCGGTAGCTc                                                                                                                                                       >  2:285673/1‑250 (MQ=255)                                                                                           aTGTTAGTACCCTGATCCGCGGTGCGGGAGTTGCCGATGATGTACGGTTTGATCGCTTCCAGACTTTCGATGAAGTGGGTCATATCGACCACCAGATCGCGTTCAATCGGGAAGTTAGCTAACGCTTCAACCTTCATACCGTCGGTGTAATTACGCAGGAAGGTTTTACATGCCAGTTTTGGCACGTTGTTAACCATCATGCCGCAGGAACCACAAATCGCCATACGGCAGGACCAGCGGTAGCTCAGGTc                                                                                                                                                  >  1:358897/1‑251 (MQ=255)                                                                                                aGTACCCTGATCCGCGGTGCGGGAGTTGCCGATGATGTACGGTTTGATCGCTTCCAGACTTTCGATGAAGTGGGTCATATCGACCACCAGATCGCGTTCAATCGGGAAGTTAGCTAACGCTTCAACCTTCATACCGTCGGTGTAATTACGCAGGAAGGTTTTACATGCCAGTTTTGGCACGTTGTTAACCATCATGCCGCAGGAACCACAAATCGCCATACGGCAGGACCAGCGGTAGCTCAGGTCCGGTg                                                                                                                                             <  2:403241/251‑1 (MQ=255)                                                                                                 gTACCCTGATCCGCGGTGCGGGAGTTGCCGATGATGTACGGTTTGATCGCTTCCAGACTTTCGATGAAGTGGGTCATATCGACCACCAGATCGCGTTCAATCGGGAAGTTAGCTAACGCTTCAACCTTCATACCGTCGGTGTAATTACGCAGGAAGGTTTTACATGCCAGTTTTGGCACGTTGTTAACCATCATGCCGCAGGAACCACAAATCGCCATACGGCAGGACCAGCGGTAGCTCAGGTCCGGTGc                                                                                                                                            <  1:79317/251‑1 (MQ=255)                                                                                                   aCCCTGATCCGCGGTGCGGGAGTTGCCGATGATGTACGGTTTGATCGCTTCCAGACTTTCGATGAAGTGGGTCATATCGACCACCAGATCGCGTTCAATCGGGAAGTTAGCTAACGCTTCAACCTTCATACCGTCGGTGTAATTACGCAGGAAGGTTTTACATGCCAGTTTTGGCACGTTGTTAACCATCATGCCGCAGGAACCACAAATCGCCATACGGCAGGACCAGCGGTAGCTCAGGTCCGGTGccc                                                                                                                                          >  1:424833/1‑250 (MQ=255)                                                                                                    cccTGATCCGCGGTGCGGGAGTTGCCGATGATGTACGGTTTGATCGCTTCCAGACTTTCGATGAAGTGGGTCATATCGACCACCAGATCGCGTTCAATCGGGAAGTTAGCTAACGCTTCAACCTTCATACCGTCGGTGTAATTACGCAGGAAGGTTTTACATGCCAGTTTTGGCACGTTGTTAACCATCATGCCGCAGGAACCACAAATCGCCATACGGCAGGACCAGCGGTAGCTCAGGTCCGGTGCCAg                                                                                                                                         <  1:315567/251‑1 (MQ=255)                                                                                                    cccTGATCCGCGGAGCGGGAGTTGCCGATGATGTACGGTTTGATCGCTTCCAGACTTTCGATGAAGTGGGTCATATCGACCACCAAATCGCGTTCAATCGGGAAGCTAGCTAACGCTTCAACCTTAATACCGTCGGTGAAATTACGCAGGAAGGATTTACATGCCAGTTTTGGCACGTTGTTAACCAACATCCCGCAGGAACCACAAATCGCCTTACGGCAGGACCACAGGCAGCTCAGGTCCGGTGcc                                                                                                                                           >  1:290328/1‑249 (MQ=255)                                                                                                         aTCCGCGGTGCGGGAGTTGCCGATGATGTACGGTTTGATCGCTTCCAGACTTTCGATGAAGTGGGTCATATCGACCACCAGATCGCGTTCAATCGGGAAGTTAGCTAACGCTTCAACCTTCATACCGTCGGTGTAATTACGCAGGAAGGTTTTACATGCCAGTTTTGGCACGTTGTTAACCATCATGCCGCAGGAACCACAAATCGCCATACGGCAGGACCAGCGGTAGCTCAGGTCCGGTGCCAGGTTGt                                                                                                                                    >  2:122941/1‑251 (MQ=255)                                                                                                           ccGCGGTTCGGAAGTTGCCGATGATGTACAGTTTGATCGCTTCCAGACTTTCGATGAAGTGGGTCATAACGACCACCAGATCGCGTTCAATCGGGAATTTAGCTAACGCTTCG‑CCTTCATACCGTCGGTGTAATTACGCAGTAAGGTTTTACATGCCAGTTTTGGCACGTTGTTAACCATCATGTCGCAGGATCCACAAATCGCCATACGGCAGGACCAGCGGTAGCTCAGGTCCGGTTCCAGGTTGTCtt                                                                                                                                 <  2:231667/251‑1 (MQ=255)                                                                                                            cgcgGTGCGGGAGTTGCCGATGATGTACGGTTTGATCGCTTCCAGACTTTCGATGAAGTGGGTCATATCGACCACCAGATCGCGTTCAATCGGGAAGTTAGCTAACGCTTCAACCTTCATACCGTCGGTGTAATTACGCAGGAAGGTTTTACATGCCAGTTTTGGCACGTTGTTAACCATCATGCCGCAGGAACCACAAATCGCCATACGGCAGGACCAGCGGTAGCTCAGGTCCGGTGCCAGGTTGTCtt                                                                                                                                 >  1:123616/1‑251 (MQ=255)                                                                                                                          tGCCGATGATGTACGGTTTGATCGCTTCCAGACTGTCGATGAAGTGGGGCATATCGACCACCAGATCACGTTCAACCGGGAAGTTAGCTAACGCATCAACCTTCATACCGTCGGTGTAATTACGCATGAAGGTTATACATGCCAGTTTTGGCACGTTGTTAACCATCATGCCGCAGGAACCACAAATCGCCATACGGCAGTACCACCGGTAGCTCAGGTCCGGTGCCAGGTTGTCTTTGATGTAGCCCAg                                                                                                                    <  1:277437/250‑1 (MQ=255)                                                                                                                                  atgTACGGTTTGATCGCTTCCAGACTTTCGAGGAAGTGGGTCATATCGACCACCAGATCGCGTTCAATCGGGAAGTTAGCTAACGCTTCAACCTTCATACCGTCGGTGTAATTACGCAGGAAGGTTTTACATGCCAGTTTTGGCACGTTGTTAACCATCATGCCGCAGGAACCACAAATCGCCATACGGCAGGACCAGCGGTAGCTCAGGTCCGGTGCCAGGTTGTCTTTGATGTAGCCCAGCGCATCCAg                                                                                                           <  2:109508/251‑1 (MQ=255)                                                                                                                                    gTACGGTTTGATCGCTTCCAGGCTTTCGATGAAGTGGGTCATATCGACCACCAGATCGCGTTCAATCGGGAAGTTAGCTAACGCTTCAACCTTCATACCGTCGGTGTAATTACGCAGGAAGGTTTTACATGCCAGTTTTGGCACGTTGTTAACCATCATGCCGCAGGAACCACAAATCGCCATACGGCAGGACCAGCGGTAGCTCAGGTCCGGTGCCAGGTTGTCTTTGATGTAGCCCAGCGCATCCAGTa                                                                                                         <  1:285673/251‑1 (MQ=255)                                                                                                                                    gTACCGTTTGATCGCTTCCAGACTTTCGATGAAGTGGGTCATATCGACCACCAGATCGCGTTCAATCGGGAAGTTAGCTAACGCTTCAACCTTCATACCGTCGGTGTAATTACGCAGGAAGGTTTTACATGCCAGTTTTGGCACGTTGGTAACCATCATGCCGCAGGAACCACAAATCGCCATACGGCAGGACCAGCGGTAGCTCAGGTCCGGTGCCAGGTTGTCTTTGATGTAGCCCAGCGCATCCAGt                                                                                                          <  1:450371/250‑1 (MQ=255)                                                                                                                                                  cTTCCAGACTTTCGATGAAGTGGGTCATATCGACCACCAGATCGCGTTCAATCGGGAAGTTAGCTAACGCTTCAACCTTCATACCGTCGGTGTAATTACGCAGGAAGGTTTTACATGCCAGTTTTGGCACGTTGTTAACCATCATGCCGCAGGAACCACAAATCGCCATACGGCAGGACCAGCGGTAGCTCAGGTCCGGTGCCAGGTTGTCTTTGATGTAGCCCAGCGCATCCAGTAATGAGGTAGTTGCg                                                                                           >  1:271110/1‑251 (MQ=255)                                                                                                                                                            ttCGATGAAGTGGGTCATATCGACCACCAGATCGCGTTCAATCGGGAAGTTAGCTAACGCTTCAACCTTCATACCGTCGGTGTAATTACGCAGGAAGGTTTTACATGCCAGTTTTGGCACGTTGTTAACCATCATGCCGCAGGAACCACAAATCGCCATACGGCAGGACCAGCGGTAGCTCAGGACCGGTGCCAGGTTGTCTTTGATGTAGACCAGCGCATCCAGTAATTAGGTAGTTGGGTCTTAAGGc                                                                                  >  2:130600/1‑250 (MQ=255)                                                                                                                                                                       gggTCATATCGACCACCAGATCGCGTTCAATCGGGAAGTTAGCTAACGCTTCAACCTTCATACCGTCGGTGTAATTACGCAGGAAGGTTTTACATGCCAGTTTTGGCACGTTGTTAACCATCATGCCGCAGGAACCACAAATCGCCATACGGCAGGACCAGCGGTAGCTCAGGTCCGGTGCCAGGTTGTCTTTGATGTAGCCCAGCGCATCCAGTAATGAGGTAGTTGCGTCATAAGGCACTTCATAGAAt                                                                      >  1:177631/1‑251 (MQ=255)                                                                                                                                                                           cATATCGACCACCAGATCGCGTTCAATCGGGAAGTTAGCTAACGCTTCAACCTTCATACCGTCGGTGTAATTACGCAGGAAGGTTTTACATGCCAGTTTTGGCACGTTGTTAACCATCATGCCGCAGGAACCACAAATCGCCATACGGCAGGACCAGCGGTAGCTCAGGTCCGGTGCCAGGTTGTCTTTGATGTAGCCCAGCGCATCCAGTAATGAGGTAGTTGCGTCATAAGGCACTTCATAGAATgcgc                                                                  <  1:132166/251‑1 (MQ=255)                                                                                                                                                                           cATATCGACCACCAGATCGCGTTCAATCGGGAAGTTAGCTAACGCTTCAACCTTCATACCGTCGGTGTAATTACGCAGGAAGGTTTTACATGCCAGTTTTGGCACGTTGTTAACCATCATGCCGCAGGAACCACAAATCGCCATACGGCAGGACCAGCGGTAGCTCAGGTCCGGTGCCAGGTTGTCTTTGATGTAGCCCAGCGCATCCAGTAATGAGGTAGTTGCGTCATAAGGCACTGCATAGAATgcg                                                                   <  2:177631/250‑1 (MQ=255)                                                                                                                                                                           cATATCGACCACCAGAGCGCGTTCAATCGGGAAGTTAGCTAACGCTTCAACCTTCATACCGTCGGTGTAATTACGCAGGAAGGTGTTACAGGCCAGTGTTGGCACGTTGTTAACCATCATGCCGCAGGAGCCACAAATCGCCATACGGGAGGACCAGCGGTAGCTCAGGTCCGGTGCCAGGTTGTCTTTGATGTAGCCCAGCGCATCCAGTAATGAGGTAGTTGCGTCATAAGGCACTTCATAGAATgcg                                                                   <  1:454224/250‑1 (MQ=255)                                                                                                                                                                              atCGACCACCAGATCGCGTTCAATCGGGAAGTTAGCTAACGCTTCAACCTTCATACCGTCGGTGTAATTACGCAGGAAGGTTTTACATGCCAGTTTTGGCACGTTGTTAACCATCATGCCGCAGGAACCACAAATCGCCATACGGCAGGACCAGCGGTAGCTCAGGTCCGGTGCCAGGTTGTCTTTGATGTAGCCCAGCGCa                                                                                                                <  2:108570/202‑1 (MQ=255)                                                                                                                                                                              atCGACCACCAGATCGCGTTCAATCGGGAAGTTAGCTAACGCTTCAACCTTCATACCGTCGGTGTAATTACGCAGGAAGGTTTTACATGCCAGTTTTGGCACGTTGTTAACCATCATGCCGCAGGAACCACAAATCGCCATACGGCAGGACCAGCGGTAGCTCAGGTCCGGTGCCAGGTTGTCTTTGATGTAGCCCAGCGCa                                                                                                                >  1:108570/1‑202 (MQ=255)                                                                                                                                                                                cGACCACCAGATCGCGTTCAATCGGGAAGTTAGCTAACGCTTCAACCTTCATACCGTCGGTGTAATTACGCAGGAAGGTTTTACATGCCAGTTTTGGCACGTTGTTAACCATCATGCCGCAGGAACCACAAATCGCCATACGGCAGGACCAGCGGTAGCTCAGGTCCGGTGCCAGGTTGTCTTTGATGTAGCCCAGCGCATCCAGTAATGAGGTAGTTGCGTCATAAGGCACTTCATAGAATGCGCTAtgc                                                             <  2:246343/251‑1 (MQ=255)                                                                                                                                                                                cGACCACCAGATCGCGTTCAATCGGGAAGTTAGCTAACGCTTCAACCTTCATACCGTCGGTGTAATTACGCAGGAAGGTTTTACATGCCAGTTTTGGCACGTTGTTAACCATCATGCCGCAGGAACCACAAATCGCCATACGGCAGGACCAGCGGTAGCTCAGGTCCGGTGCCAGGTTGTCTTTGATGTAGCCCAGCGCATCCAGTAATGAGGTAGTTGCGTCATAAGGCACTTCATAGAATGCGCTAtg                                                              <  1:146660/250‑1 (MQ=255)                                                                                                                                                                                  accaccAGATCGCGTTCAATCGGGAAGTTAGCTAACGCTTCAACCTTCATACCGTCGGTGTAATTACGCAGGAAGGTTTTACATGCCAGTTTTGGCACGTTGTTAACCATCATGCCGCAGGAACCACAAATCGCCATACGGCAGGACCAGCGGTAGCTCAGGTCCGGTGCCAGGTTGTCTTTGATGTAGCCCAGCGCATCCAGTAATGAGGTAGTTGAGTCATAAGGCACTTCATAGAATGCGCTAtgcgg                                                           >  2:366582/1‑251 (MQ=255)                                                                                                                                                                                   ccaccaGATCGCGTTCAATCGGGAAGTTAGCTAACGCTTCAACCTTCATACCGTCGGTGTAATTACGCAGGAAGGTTTTACATGCCAGTTTTGGCACGTTGTTAACCATCATGCCGCAGGAACCACAAATCGCCATACGGCAGGACCAGCGGTAGCTCAGGTCCGGTGCCAGGTTGTCTTTGATGTAGCCCAGCGCATCCAGTAATGAGGTAGTTGCGTCATAAGGCACTTCATAGAATGCGCTAtgcggg                                                          >  2:72013/1‑250 (MQ=255)                                                                                                                                                                                          aTCGCGTTCAATCGGGAAGTTAGCTAACGCTTCAACCTTCATACCGTCGGTGTAATTACGCAGGAAGGTTTTACATGCCAGTTTTGGCACGTTGTTAACCATCATGCCGCAGGAACCACAAATCGCCATACGGCAGGACCAGCGGTAGCTCAGGTCCGGTGCCAGGTTGTCTTTGATGTAGCCCAGCGCATCCAGTAATGAGGTAGGTGCGTCATAAGGCACTTCATAGAATGCGCTATGCGGTGCGGTAt                                                   >  2:378522/1‑251 (MQ=255)                                                                                                                                                                                                   aaTCGGGAAGTTAGCTAACGGTTCAACCTTCATTCCGTCGGTGTAATTACGCAGGAAGGTTTTACATGCCAGTTTTGGCACGTTGTTAACCATCATGCCGCAGGAACCACAAATCGCCATACGGCAGGACCAGCGGTAGCTCAGGTCCGGTGCCAGGTTGTCTTTGATGTAGCCCAGCGCATCCAGTAATGAGGTAGTTGCGTCATAAGGCACTTGATAGAAGGCGTTATGCGGTGCGGTATCGACTTCCg                                          <  1:399092/251‑1 (MQ=255)                                                                                                                                                                                                      cGGGAAGTTAGCTAACGCTTCAACCTTCATACCGTCGGTGTAATTACGCAGGAAGGTTTTACATGCCAGTTTTGGCACGTTGTTAACCATCATGCCGCAGGAACCACAAATCGCCATACGGCAGGACCAGCGGTAGCTCAGGTCCGGTGCCAGGTTGTCTTTGATGTAGCCCAGCGCATCCAGTAATGAGGTAGTTGCGTCATAAGGCACTTCATAGAATGCGCTATGCGGTGCGGTATCGACTTCCGGGt                                       <  1:55525/251‑1 (MQ=255)                                                                                                                                                                                                       gggAAGTTAGCTAACGCTTCAACCTTCATACCGTCGGTGTAATTACGCAGGAAGGTTTTACATGCCAGTTTTGGCACGTTGTTAACCATCATGCCGCAGGAACCACAAATCGCCATACGGCAGGACCAGCGGTAGCTCAGGTCCGGTGCCAGGTTGTCTTTGATGTAGCCCAGCCCATCCAGCAATGAGGTAGTTGCGGCATAAGGCACTTCATAGAATGCGCTATGCGGTGCGGTATCGACTTCCGGGtt                                      >  2:363116/1‑251 (MQ=255)                                                                                                                                                                                                              tAGCTAACGCTTCAACCTTCATACCGTCGGTGTAATTACGCAGGAAGGTTTTACATGCCAGTTTTGGCACGTTGTTAACCATCATGCCGCAGGAACCACAAATCGCCATACGGCAGGACCAGCGGTAGCTCAGGTCCGGTGCCAGGTTGTCTTTGATGTAGCCCAGCGCATCCAGTAATGAGGTAGTTGCGTCATAAGGCACTTCATAGAATGCGCTATGCGGTGCGGTATCGACTTCCGGGTTATAgcgc                               >  2:207925/1‑251 (MQ=255)                                                                                                                                                                                                                      gCTTCAACCTTCATACCGTCGGTGTAATTACGCAGGAAGGTTTTACATGCCAGTTTTGGCACGTTGTTAACCATCATGCCGCAGGAACCACAAATCGCCATACGGCAGGACCAGCGGTAGCTCAGGTCCGGTGCCAGGTTGTCTTTGATGTAGCCCAGCGCATCCAGTAATGAGGGAGTTGCGTCATAAGGCACTTCCTAGAATGCGCTATGCGGTGCGGTATCGACTTCCGGGTTATAGCGCACCACCTc                       >  2:34715/1‑251 (MQ=255)                                                                                                                                                                                                                         tCAACCTTCATACCGTCGGTTTAATTACGCAGGAAGGTTTTACATGCCAGTTTTGGCACGATGTTAACCATCATGCCGCAGGAACCACAAATCGCCATACGGCAGGAGCAACGGTAGTCCTGGTCCGGTGCCAGGTTGTCTTTGATGGAGCCCAGCGCATCCAGTAATGAGGTAGTGGCGTCATAAGGGACTTCATAGAATGGGCTATTCGGTGCGGTATCGACTTTCGGGTtatattgaaatacataaat                    >  2:208325/1‑236 (MQ=255)                                                                                                                                                                                                                          cAACCTTCATACCGTCGGTGTAATTACGCAGGAAGGTTTTACATGCCAGTTTTGGCACGTTGTTAACCATCATGCCGCAGGAACCACAAATCGCCATACGGCAGGACCAGCGGTAGCTCAGGTCCGGTGCCAGGTTGTCTTTGATGTAGCCCAGCGCATCCAGTAATGAGGTAGTTGCGTCATAAGGCACTTCATAGAATGCGCTATGCGGTGCGGTATCGACTTCCGGGTTATAGCGCACCACCTCAAt                    <  2:369520/250‑1 (MQ=255)                                                                                                                                                                                                                                tCATACCGTCGGTGTAATTACGCAGGAAGGTTTTACATGCCAGTTTTGGCACGTTGTTAACCATCATGCCGCAGGAACCACAAATCGCCATACGGCAGGACCAGCGGGAGCTCAGGTCCGGTGCCAGGTTGTCTTTGATGTAGCCCAGCGCATCCAGTAATGAGGGAGTTGCGTCATAAGGCACTTCATCGAATGCGCTATGCGGTGCGGTATCGACTTCCGGGTTATAGCGCACCACCTCAATTTTCAgg             >  1:216520/1‑251 (MQ=255)                                                                                                                                                                                                                                       gTCGGTGTAATTACGCAGGAAGGTTTTACATGCCAGTTTTGGCACGTTGTTAACCATCATGCCGCAGGAACCACAAATCGCCATACGGCAGGACCAGCGGTAGCTCAGGTCCGGTGCCAGGTTGTCTTTGATGTAGCCCAGCGCATCCAGTAATGAGGTAGTTGCGTCATAAGGCACTTCATAGAATGCGCTATGCGGTGCGGTATCGACTTCCGGGTTATAGCGCACCACCTCAATTTTCAGGTTTTTc       >  1:214442/1‑250 (MQ=255)                                                                                                                                                                                                                                         cGGTGTAATTACGCAGGAAGGTTTTACATGCCAGTTTTGGCACGTTGTTAACCATCATGCCGCAGGAACCACAAATCGCCATACGGCAGGACCAGCGGTAGCTCAGGTCCGGTGCCAGGTTGTCTTTGATGTAGCCCAGCGCATCCAGTAATGAGGTAGTTGCGTCATAAGGCACTTCATAGAATGCGCTATGCGGTGCGGTATCGACTTCCGGGTTATAGCGCACCACCTCAATTTTCAGGTTTTTCAtc    >  2:368541/1‑251 (MQ=255)                                                                                                                                                                                                                                          ggTGTAATTACGCAGGAAGGTTTTACATGCCCGTTTTGGCACGTTGTTAACCATCATGCCGCAGGAACCACAAATCGCCATACGGCAGGACCAGCGGTAGCTCAGGTCCGGTGCCAGGTTGTCTTTGATGTAGCCCAGCGCATCCAGTAATGAGGTAGTTGCGTCATAAGGCACTTCATAGAATGCGCTATGCGGTGCGGTATCGACTTCCGGGTTATAGCGCACCACCTCAATTTTCAGGTTTTTCAtct   <  1:69029/251‑1 (MQ=255)                                                                                                                                                                                                                                           gtgtAATTACGCAGGAAGGTTTTACATGCCAGTTTTGGCACGTTGTTAACCATCATGCCGCAGGAACCACAAATCGCCATACGGCAGGACCAGCGGTAGCTCAGGTCCGGTGCCAGGTTGTCTTTGATGTAGCCCAGCGCATCCAGTAATGAGGTAGTTGCGTCATAAGGCACTTCATAGAATGCGCTATGCGGTGCGGTATCGACTTCCGGGTTATAGCGCACCACCTCAATTTTCAGGTTTTTCAtctc  >  2:330787/1‑251 (MQ=255)                                                                                                                                                                                                                                           gtgtAATTACGCAGGAAGGTTTTACATGCCAGTTTTGGCACGTTGTTAACCATCATGCCGCAGGAACCACAAATCGCCATACGGCAGGACCAGCGGTAGCTCAGGTCCGGTGCCAGGTTGTCTTTGATGTAGCCCAGCGCATCCAGTAATGAGGTAGTTGCGTCATAAGGCACTTCATAGAATGCGCTATGCGGTGCGGTATCGACTTCCGGGTTATAGCGCACCACCTCAATTTTCAGGTTTTTCAtctc  <  1:207925/251‑1 (MQ=255)                                                                                                                                                                                                                                            tgtAATTACGCAGGAAGGTTTTACATGCCAGTTTTGGCACGTTGTTAACCATCATGCCGCAGGAACCACAAATCGCCATACGGCAGGACCAGCGGTAGCTCAGGTCCGGTGCCAGGTTGTCTTTGATGTAGCCCAGCGCATCCAGTAATGAGGTAGTTGCGTCATAAGGCACTTCATAGAATGCGCTATGCGGTGCGGTATCGACTTCCGGGt                                       <  2:362021/213‑1 (MQ=255)                                                                                                                                                                                                                                            tgtAATTACGCAGGAAGGTTTTACATGCCAGTTTTGGCACGTTGTTAACCATCATGCCGCAGGAACCACAAATCGCCATACGGCAGGACCAGCGGTAGCTCAGGTCCGGTGCCAGGTTGTCTTTGATGTAGCCCAGCGCATCCAGTAATGAGGTAGTTGCGTCATAAGGCACTTCATAGAATGCGCTATGCGGTGCGGTATCGACTTCCGGGt                                       >  1:362021/1‑213 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                    AGGCCAAACTGCGGGCACGCGGCGTAGCACAAACCACAGTTGATGCAACCGGAGAACTGGTGATACTTCGCCATCTGCGCCGGGGTCTGGATGTTAGTACCCTGATCCGCGGTGCGGGAGTTGCCGATGATGTACGGTTTGATCGCTTCCAGACTTTCGATGAAGTGGGTCATATCGACCACCAGATCGCGTTCAATCGGGAAGTTAGCTAACGCTTCAACCTTCATACCGTCGGTGTAATCACGCAGGAAGGTTTTACATGCCAGTTTTGGCACGTTGTTAACCATCATGCCGCAGGAACCACAAATCGCCATACGGCAGGACCAGCGGTAGCTCAGGTCCGGTGCCAGGTTGTCTTTGATGTAGCCCAGCGCATCCAGTAATGAGGTAGTTGCGTCATAAGGCACTTCATAGAATGCGCTATGCGGTGCGGTATCGACTTCCGGGTTATAGCGCACCACCTCAATTTTCAGGTTTTTCATCTC  >  NC_000913/4380027‑4380511

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑