Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,414,650	C→T	Q126* (CAG→TAG)	aidB →	putative acyl‑CoA dehydrogenase AidB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,414,650	0	C	T	98.2%	177.0 / NA	56	Q126* (CAG→TAG)	aidB	putative acyl‑CoA dehydrogenase AidB
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base T (33/22);  total (34/22)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGGGCGGCTGGCGAATGTGAATCCGCCTGAATTATTGCGCTACGATGCGCAAGGACGCCGTCTGGACGATGTGCGTTTTCACCCCGCCTGGCACCTGCTGATGCAGGCGCTATGTACCAATCGGGTGCACAATCTTGCCTGGGAAGAAGACGCTCGCTCCGGCGCATTTGTGGCGCGCGCGGCGCGTTTTATGTTACATGCGCAGGTTGAGGCAGGGTCGT‑TATGTCCGATAACCATGA‑CCTTTGCCGCCACGCCATTGTTGTTACAGATGTTACCCGCGCCGTTTCAGGACTGGACCACGCCGCTATTGAGCGATCGCTACGATTCTCACTTATTGCCAGGTGGGCAAAAACGCGGTTTGTTGATTGGCATGGGAATGACGGAAAAGCAGGGCGGTTCCGATGTTATGAGCAACACCACCCGTGCAGAGCGTCTGGAAGATGGC  >  NC_000913/4414410‑4414892                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                     aTTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGGGCGGCTGGCGAATGTGAATCCGCCTGAATTATTGCGCTACGATGCGCAAGGACGCCGTCTGGACGATGTGCGTTTTCACCCCGCCTGGCACCTGCTGATGCAGGCGCTATGTACCAATCGGGTGCACAATCTTGCCTGGGAAGAAGACGCTCGCTCCGGCGCATTTGTGGCGCGCGCGGCGCGTTTTATGTTACATGCGTATGTTGAGGc                                                                                                                                                                                                                                            <  2:26137/251‑1 (MQ=255)    ggTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGGGCGGCTGGCGAATGTGAATCCGCCTGAATTATTGCGCTACGATGCGCAAGGACGCCGTCTGGACGATGGGCGTTTTCACCCCGCCTGGCACCTGCTGATGCAGGCGCTATGTACCAATCGGGTGCACAATCTTGCCTGGGAAGAAGACGCTCGCTCCGGCGCATTTGTGGCGCGCGCGGCGCGTTTTATGTTACATGCGTAGGTTGAGGCAgg                                                                                                                                                                                                                                         >  1:299724/1‑251 (MQ=255)     gTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGGGCGGCTGGCGAATGTGAATCCGCCTGAATTATTGCGCTACGATGCGCAAGGACGCCGTCTGGACGATGTGCGTTTTCACCCCGCCTGGCACCTGCTGATGCAGGCGCTATGTACCAATCGGGTGCACAATCTTGCCTGGGAAGAAGACGCTCGCTCCGGCGCATTTGTGGCGCGCGCGGCGCGTTTTATGTTACATGCGTAGGTTGAGGCAggg                                                                                                                                                                                                                                        >  2:196306/1‑251 (MQ=255)       cagcagTTAGGAACGGCTGAATCCCTTGAACTGGGGCGGCTGGTGAATGTGAATCCGCCTGAATTATTGCGCTACGATGCGCAAGGACGCCGTCTGGACGATGTGCGTTTTCACCCCGCCTGGCACCTGCTGATGCAGGCGCTATGTACCAATCGGGTGCACAATCTTGCCTGGGAAGAAGACGCTCGCTCCGGCGCATTTGTGGCGCGCGCGGCGCGTTTTATGTTACATGCGTAGGTTGAGGCAGGGTc                                                                                                                                                                                                                                      <  1:378958/251‑1 (MQ=255)         gcagTTAGGAACGGCTGAATCCCTTGAACTGGGGCGGCTGGCGAATGTGAATCCGCCTGAATTATTGCGCTACGATGCGCAAGGACGCCGTCTGGACGATGTGCGTTTTCACCCCGCCTGGCACCTGCTGATGCAGGCGCTATGTACCAATCGGGTGCACAATCTTGCCTGGGAAGAAGACGCTCGCTCCGGCGCATTTGTGGCGCGCGCGGCGCGTTTTATGTTACATGCGTAGGTTGAGGCAGGGGCGt                                                                                                                                                                                                                                    >  1:73971/1‑251 (MQ=255)         gcagTTAGGAACGGCTGAATCCCTTGAACTGGGGCGGCTGGCGAATGTGAATCCGCCTGAATTATTGCGCTACGATGCGCAAGGACGCCGTCTGGACGATGTGCGTTTTCACCCCGCCTGGCACCTGCTGATGCAGGCGCTATGTACCAATCGGGTGCACAATCTTGCCTGGGAAGAAGACGCTCGCTCCGGCGCATTGGTGGCGCGCGCGGCGCGTTTTATGTTACATGCGTAGGTTGAGGCAGGGTCGt                                                                                                                                                                                                                                    <  1:431442/251‑1 (MQ=255)          cagTTAGGAACGGCTGAATCCCTTGAACTGGGGCGGCTGGCGAATGTGAATCCGCCTGAATTATTGCGCTACGATGCGCAAGGACGCCGTCTGGACGATGTGCGTTTTCACCCCGCCTGGCACCTGCTGATGCAGGCGCTATGTACCAATCGGGTGCACAATCTTGCCTGGGAAGAAGACGCTCGCTCCGGCGCATTTGTGGCGCGCGCGGCGCGTTTTATGTTACATGCGTAGGTTGAGGCAGGGTCGt‑t                                                                                                                                                                                                                                  >  1:216626/1‑251 (MQ=255)               aGGAACGGCTGAATTCCTTGAACTGGGGCGGCTGGCGAATGTGAATCCGCCTGAATTATTGCGCTACGATGCGCAAGGACGCCGACTGGACGATGTGCGCTTTCACCCCGCCTGGCACGTGCTGTTGCAGGCGCTATGTACCAATCGGTTGCACAATCTTGCCTGGGGAGAAGACGCTCGCTCCGGAGCATTTGTAGCGCGCGCGGCGCGATTTATGATACATGCGTCGTTTGATGCAGGGTCGT‑TAtatc                                                                                                                                                                                                                             >  2:164643/1‑248 (MQ=255)                        tGAATCCCTTGAACTGGGGCGGCTGGCGAATGTGAATCCGCCTGAATTATTGCGCTACGATGCGCAAGGACGCCGTCTGGACGATGTGCGTTTTCACCCCGCCTGGCACCTGCTGATGCAGGCGCTATGTACCAATCGGGTGCACAATCTTGCCTGGGAAGAAGACGCTCGCTCCGGCGCATTTGTGGCGCGCGCGGCGCGTTTTATGTTACATGCGTAGGTTGAGGCAGGGTCGT‑TATGTCCGATAAcc                                                                                                                                                                                                                     >  2:250273/1‑250 (MQ=255)                                                   gAATGTGAATCCGCCTGAATTATTGCGCTACGATGCGCAAGGACGCCGTCTGGACGATGTGCGTTTTCACCCCGCCTGGCACCTGCTGATGCAGGCGCTATGTACCAATCGGGTGCACAATCTTGCCTGGGAAGAAGACGCTAGCTCCGGCGCATTTGTGGCGCGCGCGGCGCGTTTTATGTTACATGCGTAGGTTGAGGCAGGGTCGT‑TATGTCAGATAACCATGA‑CCTTTGCCGCCACGCCATtgctgt                                                                                                                                                                                        >  1:336334/1‑251 (MQ=255)                                                   gAATGAGAACCCGCCTGAATTATTGCGCTACGATGCGCAAGGACGCCGTCTGGACGCTGTGCGTTTTAACCCCGCCTGGCACCTGCTGCTGCAAGCGATATGTACCAATCGGGTGCACAATCTTGCCTGGGAAGAAGACGCTCGCTCCGGCGCATTTGGGGCGCGCGCGGCGCGTTTTATGTTACATGAGTAGGTTGAGGAAGGGTCGT‑TATGTCCGCTAACCATGA‑ACTTTGCCGCCCCGCCTTtgttgt                                                                                                                                                                                        >  2:153027/1‑251 (MQ=255)                                                            tCCGCCTGCATTATTGCGCTCCGATGCGCAAGGACGCCGTCTGGCCGATGTGCGTTTTCACCCCGCCAGGCACCTGCTGATGCAGGCGCTATGTACCAATCGGGTGCACAATCTTGCCTGGGAAGAAGACGCTCGCTCCGGCGCACTTGTGGCGCGCGCGGCGCGTTTTATGTTACATGCGCAGGTTGAGGCAGGGTCGT‑TATGTCCGCTAACCATGA‑CCTTTGCCGCCCCGCCATTGTTGTTACAGATGt                                                                                                                                                                               >  2:256107/1‑251 (MQ=255)                                                               gCCTGAATTATTGCGCTACGATGCGCAAGGACGCCGTCTGGACGATGTGCGTTTTCACCCCGCCTGGCACCTGCTGATGCAGGCGCTATGTACCAATCGGGTGCACAATCTTGCCTGGGAAGAAGACGCTCGCTCCGGCGCATTTGTGGCGCGCGCGGCGCGTTTTATGTTACATGCGTAGGTTGAGGCAGGGTCGT‑TATGTCCGATAACCATGA‑CCTTTGCCGCCACGCCATTGTTGTTACAGATGTTAc                                                                                                                                                                            <  1:153618/251‑1 (MQ=255)                                                                  tGAATTATTGCGCTACGATGCGCAAGGACGCCGTCTGGACGATGTGCGTTTTCACCCCGCCTGGCACCTGCTGATGCAGGCGCTATGTACCAATCGGGTGCACAATCTTGCCTGGGAAGAAGACGCTCGCTCCGGCGCATTTGTGGCGCGCGCGGCGCGTTTTATGTTACATGCGTAGGTTGAGGCAGGGTCGT‑TATGTCCGATAACCATGA‑CCTTTGCCGCCACGCCATTGTTGTTACAGATGTTACCcg                                                                                                                                                                         >  2:37255/1‑251 (MQ=255)                                                                      ttattGCGCTACGATGCGCAAGGACGCCGTCTGGACGATGTGCGTTTTCACCCCGCCTGGCACCTGCTGATGCAGGCGCTATGTACCAATCGGGTGCACAATCTTGCCTGGGAAGAAGACGCTCGCTCCGGCGCATTTGTGGCGCGCGCGGCGCGTTTTATGTTACATGCGTAGGTTGAGGCAGGGTCGT‑TATGTCCGATAACCCTGA‑CCTTTGCCGCCACGCCATTGTTGTTACAGATGTTACCcgcggc                                                                                                                                                                     >  2:386977/1‑249 (MQ=255)                                                                        attGCGCTACGCTGCGCAAGGACGCCGTCTGGACAATGTGCGTTTTCACCCCGCCTGGCACCTGCTGATGCAGGCGCTATGTACCAATCGGGTGCACAATCTTGCCTGGGAAGAAGACGCTCGCTCCGGCGCATTTGTGGCGCGCGCGGCGCGTTTTATGTTACATGCGTAGGTTGAGGCAGGGTCGT‑TATGTCCGATAACCATGA‑CCTTTGCCGCCACGCCCTTGTTGTTACAGATGTTACCCGCGCCGt                                                                                                                                                                   >  2:254753/1‑251 (MQ=255)                                                                        attGCGCTACGATGCGCAAGGACGCCGTCTGGACGATGTGCGTTTTCACCCCGCCTGGCACCTGCTGATGCCGGCGCTATGTACCAATCGGGTGCACAATCTTGCCTGGGAAGAAGACGCTCGCTCCGGCGCATTTGTGGCGCGCGCGGCGCGTTTTATGTTACATGCGTAGGGTGAGGCAGGGGCGT‑TATGTCCGATAACCCTGA‑CCTTTTCCGCCACGCCATTGTTGTTACAGATGTTAccccggccgt                                                                                                                                                                   >  2:10296/1‑244 (MQ=255)                                                                        attGCGCTACGATGCGCAAGGACGCCGTCTGGACGATGTGCGTTTTCACCCCGCCTGGCACCTGCTGATGCAGGCGCTATGTACCAATCGGGTGCACAATCTTGCCTGGGAAGAAGACGCTCGCTCCGGCGCATTTGTGGCGCGCGCGGCGCGTTTTATGTTACATGCGTAGGTTGAGGCAGGGTCGT‑TATGTCCGATAACCATGA‑CCTTTGCCGCCACGCCATTGTTGTTACAGATGTTACCCGCGCCg                                                                                                                                                                    <  1:185776/250‑1 (MQ=255)                                                                               tACGATGCGCAAGGACGCCGTCTGGACGATGTGCGTTTTCACCCCGCCTGGCACCTGCTGATGCAGGCGCTATGTACCAATCGGGTGCACAATCTTGCCTGGGAAGAAGACGCTCGCTCCGGCGCATTTGTGGCGCGCGCGGCGCGTTTTATGTTACATGCGTAGGTTGAGGCAGGGTCGT‑TATGTCCGATAACCATGA‑CCTTTGCCGCCACGCCATTGTTGTTACAGATGTTACCCGCGCCGTTTCAgg                                                                                                                                                             >  1:53357/1‑250 (MQ=255)                                                                                aCGATGCGCAAGGACGCCGTCTGGACGATGTGCGTTTTCCCCCCGCCTGGCACCTGCTGATGCAGGCGCTATGTACCAATCGGGTGCACAATCTTGCCTGGGAATAAGCCGCTCGCTCCGGCGCATTTGTGGCGCGCGTGGCGCGTTTTATGTTACATGCGTAGGTTGAGGCAGGGTCGT‑TATGTCCGATGACCATGA‑CCTTTGCCGCCACGCCATTGTTGTTACAGATGTTACCCGCGCCGTTTCAGGAc                                                                                                                                                           <  1:319280/251‑1 (MQ=255)                                                                                  gATGCGCAAGGACGCCGTCTGGACGATGAGCGTTTTCACCCCGCCTGGCACCTGCTGATGCAGGCGCTATGTACCAATCGGGTGCACAATCTTGCCTGGGAAGAAGACGCTCGCTCCGGCGCATTTGTGGCGCGCGCGGCGCGTTTTATGTTACATGCGTAGGTTGAGGCAGGGTCGT‑TATGTCCGATAAACATGA‑CCTTTGCCGCCACGCCATTGTTGTTACAGATGTTACCCGCGCCGTTTCAGGaatg                                                                                                                                                         >  2:430026/1‑248 (MQ=255)                                                                                    tGCGCAAGGACGCCGTCTGGACGATGTGCGTTTTCACCCCGCCTGGCACCTGCTGATGCAGGCGCTATGTACCAATCGGGTGCACAATCTTGCCTGGGAAGAAGACGCTCGCTCCGGCGCATTTGTGGCGCGCGCGGCGCGTTTTATGTTACATGCGTAGGTTGAGGCAGGGTCGT‑TATGTCCGATAACCATGA‑CCTTTGCCGCCACGCCATTGTTGTTACAGATGTTACCCGCGCCGTTTCAGGACTgg                                                                                                                                                        >  2:122042/1‑250 (MQ=255)                                                                                     gcgcAAGGACGCCGTCTGGACGATGTGCGTTTTCACCCCGCCTGGCACCTGCTGATGCAGGCGCTATGTACCAATCGGGTGCACAATCTTGCCTGGGAAGAAGACGCTCGCTCCGGCGCATTTGTGGCGCGCGCGGCGCGTTTTATGTTACATGCGTAGGTTGAGGCAGGGTCGT‑TATGTCCGATAACCATGA‑CCTTTGCCGCCACGCCATTGTTGTTACAGATGTTACCCGCGCCGTTTCAGGACTGGa                                                                                                                                                       <  1:96969/250‑1 (MQ=255)                                                                                         aaGGCCGCCGTCTGGACGATGTGCGTTTTCACCCCGCCTGGCACCTGCTGATGCAGGCGCTATGTCCCAACCGGGTGCACAATCTTGCCTGGGAAGAAGACGCTCGCTCCGGCGCATTTGTGGCGCGCGCGGCGCGTTTTATGTTACATGCGTAGGTTGAGGCAGGGTCGT‑TATGTCCGATAACCATGA‑CCTTTGCCGCCACGCCATTGTTGTTACAGATGTTACCCGCGCCGTTTCAGGACTGGACCAcg                                                                                                                                                  <  2:53357/251‑1 (MQ=255)                                                                                                 cGTCTGGACGATGTGCGTTTTCACCCCGCCTGGCACCTGCTGATGCAGGCGCTATGTACCAATCGGGTGCACAATCTTGCCTGGGAAGAAGACGCTCGCTCCGGCGCATTTGTGGCGCGCGCGGCGCGTTTTATGTTACATGCGTAGGTTGAGGCAGGGTCGT‑TATGTCCGATAACCATGA‑CCTTTGCCGCCACGCCATTGTTGTTACAGATGTTACCCGCGCCGTTTCAGGACTGGACCACGCCGCTAtt                                                                                                                                          >  1:331649/1‑251 (MQ=255)                                                                                                      ggACGATGTGCGTTTTCACCCCGCCTGGCACCTGCTGATGCAGGCGCTATGTACCAATCGGGTGCACAATCTTGCCTGGGAAGAAGACGCTCGCTCCGGCGCATTTGTGGCGCGCGCGGCGCGTTTTATGTTACATGCGTAGGTTGAGGCAGGGTCGT‑TATGTCCGATAACCATGA‑CCTTTGCCGCCACGCCATTGTTGTTACAGATGTTACCCGCGCCGTTTCAGGACTGGACCa                                                                                                                                                    <  1:52171/236‑1 (MQ=255)                                                                                                      ggACGATGTGCGTTTTCACCCCGCCTGGCACCTGCTGATGCAGGCGCTATGTACCAATCGGGTGCACAATCTTGCCTGGGAAGAAGACGCTCGCTCCGGCGCATTTGTGGCGCGCGCGGCGCGTTTTATGTTACATGCGTAGGTTGAGGCAGGGTCGT‑TATGTCCGATAACCATGA‑CCTTTGCCGCCACGCCATTGTTGTTACAGATGTTACCCGCGCCGTTTCAGGACTGGACCa                                                                                                                                                    >  2:52171/1‑236 (MQ=255)                                                                                                      ggACGATGTGCGTTTTCACCCCGCCTGGCACCTGCTGATGCAGGCGCTATGTACCAATCGGGTGCACAATCTTGCCTGGGAAGAAGACGCTCGCTCCGGCGCATTTGTGGCGCGCGCGGCGCGTTTTATGTTACATGCGTAGGTTGAGGCAGGGTCGT‑TATGTCCGATAACCATGA‑CCTTTGCCGCCACGCCATTGTTGTTACAGATGTTACCCGCGCCGTTTCAGGACTGGACCACGCCGCTATTGAGc                                                                                                                                      <  1:34942/250‑1 (MQ=255)                                                                                                          gATGTGCGTTTTCACCCCGCCTGGCACCTGCTGATGCAGGCGCTATGTACCAATCGGGTGCACAATCTTGCCTGGGAAGAAGACGCTCGCTCCGGCGCATTTGTGGCGCGCGCGGCGCGTTTTATGTTACATGCGTAGGTTGAGGCAGGGTCGT‑TATGTCCGATAACCATGA‑CCTTTGCCGCCACGCCATTGTTGTTACAGATGTTACCCGCGCCGTTTCAGGACTGGACCACGCCGCTATTGAGCGATCg                                                                                                                                 <  2:183241/251‑1 (MQ=255)                                                                                                                 gTTTTCACCCCGCCTGGCACCTGCTGATGCAGGCGCTATGTACCAATCGGGTGCACAATCTTGCCTGGGAAGAAGACGCTCGCTCCGGCGCATTTGTGGCGCGCGCGGCGCGTTTTATGTTACATGCGTAGGTTGAGGCAGGGGCGT‑TATGTCCGATAACCATGA‑CCTTTGCCGCCACGCCATTGTTGTTACAGATGTTACCCGCGCCGGTTCCGGAATGGACCACGCCACTATTGAGCGATCGCTACgct                                                                                                                          >  2:77610/1‑249 (MQ=255)                                                                                                                  ttttGACCCCGCCTGGCCCCTGCTGATGCAGGCGCTATGAACCAATCGGGTGCACAATCTCGCATGGGAAGAAGACGCTCGCTTCGGCGCAATTATGGCGCGCGCGGCGCGTTTTATGTTACATGCGTAGGATGAGGCAGGG‑CATGTAGGTCCGATAAC‑ACGAACCATTGACGCAAAGCCATTGTTGTTACAGATGTTACCCGCTCCGTTTTAGGCCTGGCCGAAGACGCTATTGAGcacttgatacgctt                                                                                                                         >  2:262384/1‑238 (MQ=255)                                                                                                                      cgccccGCCTGGCACCTGCTGATGCAGGCGCTATGTACCAATCGGGTGCACAATCTTGCCTGGGAAGAAGACGCTCGCTCCGGCGCATTTGTGGCGCGCGCGGCGCGTTTTATGTTACATGCGTAGGTTGAGGCAGGGTCGT‑TATGTCCGATAACCATGA‑CCTTTGCCGCCACGCCATTGTTGTTACAGATGTTACCCGCGCCGTTTCAGGACTGGACCACGCCGCTATTGAGCGATCGCTACGATTCTCa                                                                                                                     <  2:216626/249‑1 (MQ=255)                                                                                                                      cACCCCGCCTGGCACCTGCTGATGCAGGCGCTATGTACCAATCGGGTGCACAATCTTGCCTGGGAAGAAGACGCTCGCTCCGGCGCATTTGTGGCGCGCGCGGCGCGTTTTATGTTACATGCGTAGGTTGAGGCAGGGTCGT‑TATGTCCGATAACCATGA‑CCTTTGCCGCCACGCCATTGTTGTTACAGATGTTACCCGCGCCGTTTCAGGACTGGACCACGCCGCTATTGAGCGATCGCTACGATtctc                                                                                                                      >  2:78663/1‑250 (MQ=255)                                                                                                                         cccGCCTGGCACCTGCTGATGCAGGCGCTATGTACCAATCGGGTGCACAATCTTGCCTGGGAAGAAGACGCTCGCTCCGGCGCATTTGTGGCGCGCGCGGCGCGTTTTATGTTACATGCGTAGGTTGAGGCAGGGTCGT‑TATGTCCGATAACCATGA‑CCTTTGCCGCCACGCCATTGTTGTTACAGATGTTACCCGCGCCGTTTCAGGACTGGACCACGCCGCTATTGAGCGATCGCTACGATTCTCACtt                                                                                                                  <  1:369533/251‑1 (MQ=255)                                                                                                                           cGCCTGGCACCTGCTGATGCAGGCGCTATGTACCAATCGGGTGCACAATCTTGCCTGGGAAGAAGACGCTCGCTCCGGCGCATTTGTGGCGCGCGCGGCGCGTTTTATGTTACATGCGTGGGTTGAGGCAGGGTCGT‑TATGTCCGATAACCATGA‑CCTTTGCCGCCACGCCATTGTTGTTACAGATGTTACCCGCGCCGTTTCAGGACTGGACCACGCCGCTATTGAGCGATCGCTACGATTCTCACTTAt                                                                                                                <  2:450131/251‑1 (MQ=255)                                                                                                                            gCCTGGCACCTGCTGATGCAGGCGCTATGTACCAATCGGGAGCACAATCTTGCCTGGGAAGAAGACGCTCGCTCCGGCGCATTTGTGGCGCGCGCGGCGCGTTTTATGTTAAATGCGTAGGTTGAGGCAGGGTCGT‑TATGTCCGATAACCATGA‑CCTTTGCCGCCACGCCATTGTTGTTACAGCTGTTACCCGCGCCGTTTCAGGACTGGACCAAGCCGCTATTGAGCGATCGCTCCGATTCTCACTTAtt                                                                                                               >  1:239168/1‑251 (MQ=255)                                                                                                                                   aCCTGCTGATGCAGGCGCTATGTACCAATCGGGTGCACAATCTTGCCTGGGAAGAAGACGCTCGCTCCGGCGCATTTGTGGCGCGCGCGGCGCGTTTTATGTTACATGCGTAGGTTGAGGCAGGGTCGT‑TATGTCCGATAACCATGA‑CCTTTGCCGCCACGCCATTGTTGTTACAGATGTTACCCGCGCCGTTTCAGGCCTGGACCACGCCGCTATTGAGCGATCGCTACGATTCTCACTTATTGCCAggg                                                                                                        >  2:134283/1‑250 (MQ=255)                                                                                                                                   aCCTGCTGATGCAGGCGCTATGTACCAATCGGGTGCACAATCTTGCCTGGGAAGAAGACGCTCGCTCCGGCGCATTTGTGGCGCGCGCGGCGCGTTTTATGTTACATGCGTAGGTTGAGGCAGGGTCGT‑TATGTCCGATAACCATGA‑CCTTTGCCGCCACGCCATTGTTGTTACAGATGTTACCCGCGCCGTTTCAGGACTGGACCACGCCGCTATTGAGCGATCGCTACGATTCTCACTTATTGCCAGGt                                                                                                        >  1:87024/1‑251 (MQ=255)                                                                                                                                     ctgctgATGCAGGCGCTATGTACCAATCGGGTGCACAATCTTGCCTGGGAAGAAGACGCTCGCTCCGGCGCATTTGTGGCGCGCGCGGCGCGTTTTATGTTACATGCGTAGGTTGAGGCAGGGTCGT‑TATGTCCGATAACCATGA‑CCTTTGCCGCCACGCCATTGTTGTTACAGATGTTACCCGCGCCGTTTCAGGACTGGACCACGCCGCTATTGAGCGATCGCTACGATTCTCACTTATTGCCAGGTgg                                                                                                      >  1:70139/1‑251 (MQ=255)                                                                                                                                             gCAGGCGCTATGTACCAATCGGGTGCACAATCTTGCCTGGGAAGAAGACGCTCGCTCCGGCGCATTTGTGGCGCGCGCGGCGCGTTTTATGTTACATGCGTAGGTTGAGGCAGGGTCGT‑TATGTCCGATAACCATGA‑CCTTTGCCGCCACGCCATTGTTGTTACAGATGTTACCCGCGCCGTTTCAGGACTGGACCACGCCGCTATTGAGCGATCGCTACGATTCTCACTTATTGCCAGGTGGGCAAAAAc                                                                                              <  1:70864/251‑1 (MQ=255)                                                                                                                                              cAGGCGCTATGTACCAATCGGGTGCACAATCTTGCCTGGGAAGAAGACGCTCGCTCCGGCGCATTTGTGGCGCGCGCGGCGCGTTTTATGTTACATGCGTAGGTTGAGGCAGGGTCGT‑TATGTCCGATAACCATGC‑ACTTTGCCGCCCCGCCATTGTTGTTACAGATGTTACCCGCGCCGGTTCAGGACTGGACCAAGCCGCTATTGAGCGATCGCTACGATTCTCACTTATTGCCAGGTGGGCAAAAAcg                                                                                             >  2:390495/1‑251 (MQ=255)                                                                                                                                                          aCCAATCGGGTGCACAATCTTGCCTGGGAAGAAGACGCTCGCTCCGGCGCATTTGTGGCGCGCGCGGCGCGTTTTATGTTACATGCGTAGGTTGAGGCAGGGTCGT‑TATGTCCGATAACCATGA‑CCTTTGCCGCCACGCCATTGTTGTTACAGATGTTACCCGCGCCGTTTCAGGACTGGACCACGCCGCTATTGAGCGATCGCTACGATTCTCACTTATTGCCAGGTGGGCAAAAACGCGGTTTGTTGa                                                                                  <  1:205065/250‑1 (MQ=255)                                                                                                                                                            cAATCGGGTGCACAATCTTGCCTGGGAAGAAGACGCTCGCTCCGGCGCATTTGTGGCGCGCGCGGCGCGTTTTATGTTACATGCGTAGGTTGAGGCAGGGTCGT‑TATGTCCGATAACCATGA‑CCTTTGCCGCCACGCCATTGTTGTTACAGATGTTACCCGCGCCGTTTCAGGACTGGACCACGCCGCTATTGAGCGATCGCTACGATTCTCACTTATTGCCAGGTGGGCAAAAACGCGGTTTGTTGATTg                                                                               <  1:37255/251‑1 (MQ=255)                                                                                                                                                                     gCACAATCTTGCCTGGGAAGAAGACGCTCGCTCCGGCGCATTTGTGGCGCGCGCGGCGCGTTTTATGTTACATGCGTAGGTTGAGGCAGGGTCGT‑TATGTCCGATAACCATGA‑CCTTTGCCGCCACGCCATTGTTGTTACAGATGTTACCCGCGCCGTTTCAGGACTGGACCACGCCGCTATTGAGCGATCGCTACGATTCTCACTTATTGCCAGGTGGGCAAAAACGCGGTTTGTTGATTGGCATGGGa                                                                       <  1:254753/250‑1 (MQ=255)                                                                                                                                                                      cacaATCGTGCCTGGGAAGAAGACGCTCGCTCCGGCGCATTTGTGGCGCGCGCGGCGCGTTTTATGTTACATGCGTAGGTTGAGGCAGGGACGT‑TATGTCCGATAACCATGA‑CCTTTGCCGCCACGCCATTGTTGTTACAGATGTTACCCGCGACGTTTCAGGACTGGACCACGCCGCTATTGAGCGATCGCTACGATTCTCAGTTATTGACAGGGGGGCCAAAACGCGGTTTCTTGATTGGCATGGGAAt                                                                     >  1:173936/1‑251 (MQ=255)                                                                                                                                                                         aaTCTTGCCTGGGAAGAAGACGCTCGCTCCGGCGCATTTGTGGCGCGCGCGGCGCGTTTTATGTGACATGCTTAGGTTGAGGCAGGGTCGT‑TATGTCCGATAACCATGA‑CCTTTGCCGCCACGCCATTGTTGTTACAGATGTTACCCGCGCAGTTTCAGGACTGGACCACGCCGCTATTGAGCGATCGCTACGATTCTCACTTATTGCCAGGTGGGCAAAAACGCGGTTTGTTGATTGGCATGGGAATGAc                                                                  <  1:193435/251‑1 (MQ=255)                                                                                                                                                                           tCTTGCCTGGGAAGAAGACGCTCGCTCCGGCGCATTTGTGGCGCGCGCGGCGCGTTTTATGTTACATGCGTAGGTTGAGGCAGGGTCGT‑TATGTCCGATAACCATGA‑CCTTTGCCGCCACGCCATTGTTGTTACAGATGTTACCCGCGCCGTTTCAGGACTGGACCACGCCGCTATTGAGCGATCGCTACGATTCTCACTTATTGCCAGGTGGGCAAAAACGCGGTTTGTTGATTGGCATGGGAATGACgg                                                                >  1:348276/1‑251 (MQ=255)                                                                                                                                                                            cTTGCCTGGGAAGAAGACGCTCGCTCCGGCGCATTTGTGGCGCGCGCGGCGCGTTTTATGTTACATGCGTAGGTTGAGGCAGGGTCGT‑TATGTCCGATAACCATGA‑CCTTTGCCGCCACGCCATTGTTGTTACAGATGTTACCCGCGCCGTTTCAGGACTGGACCACGCCGCTATTGAGCGATCGCTACGATTCTCACTTATTGCCAGGTGGGCAAAAACGCGGTTTGTTGATTGGCATGGGAATGACgg                                                                >  1:332989/1‑250 (MQ=255)                                                                                                                                                                              tGCCTGGGAAGAAGACGCTCGCTCCGGCGCATTTGTGGCGCGCGCGGCGCGTTTTATGTTACATGCGTAGGTTGAGGCAGGGTCGT‑TATGTCCGATAACCATGA‑CCTTTGCCGCCACGCCATTGTTGTTACAGATGTTACCCGCGCCGTTTCAGGACTGGACCACGCCGCTATTGAGCGATCGCTACGATTCTCACTTATTGCCAGGTGGGCAAAAACGCGGTTTGTTGATTGGCATGGGAATGACGGaa                                                              >  1:271902/1‑250 (MQ=255)                                                                                                                                                                                   gggAAGCAGACGCTCGCTCCGGCGCATTTGTGGCGCGCGCGGCGCGTTTTATGTTACATGCGTAGGTTGAGGCAGGGTCGT‑TATGTCCGATAACCATGA‑CCTTTGCCGCCCCGCCATTGTTGTTACAGATGTTACCCGCGCCGTTTCAGGACTGGACCACGCCGCTATTGAGCGATCGCTACGATTCTCACTTATTGCCAGGTGGGCAAAAACGCGGTTTGTTGATTGGCCTGGGAATGACGGAAAAGc                                                          >  2:19785/1‑249 (MQ=255)                                                                                                                                                                                                       ggCGCATTTGTGGCGCGCGCGGCGCGTTTTATGTTACATGCGTCGGTTGAGGCAGGGTCGT‑TATGTCCGATAACCATGA‑CCTTTGCCGCCACGCCATTGTTGTTACCGATGTTACCCGCGCCGTTTCCGGACTGGACCACGCCGCTATTGAGCGCTCGCTACGATTCTCACTTATTGCCAGGTGGGCAAAAACGCGGTTTGTTGATTGGCATGGGAATGACGGAAAAGCAGGGCGGTTCCGATGTTATgcg                                    >  2:182073/1‑249 (MQ=255)                                                                                                                                                                                                           cATTGGTGGCGCGCGCGGCTCTTTTGATGTTACATGCGTAGGTTGAGGCAGGGTCGT‑TATGTCCGATAACCATGC‑CCTTTGCCGCCACGCCATTGTTGTTACAGATGTTACCCGCGCCGTTTCAGGACTGGACCACGCCGCTATTGAGCGATCGCTACGATTCTCACTTATTGCCAGGTGGGCAAAAACGCGGTTTGTTGATTGGCATGGGAATGACGGAAAAGCAGGGCGGTTCCGATGTTATGAGCAac                                <  2:217199/251‑1 (MQ=255)                                                                                                                                                                                                              ttGTGGCGCGCGCGGCGCGTTTTATGTTACATGCGTAGGTTGAGGCAGGGTCGT‑TATGTCCGATAACCATGA‑CCTTTGCCGCCACGCCATTGTTGTTACAGATGTTACCCGCGCCGTTTCAGGACTGGACCACGCCGCTATTGAGCGATCGCTAAGATTCTCACTTATTGCCAGGTGGGCAAAAACGCGGTTTGTTGATTGGCATGGGAATGACGGAAAAGCAGGGCGGTTCCGATGTTATGAGCAacac                              <  1:196306/250‑1 (MQ=255)                                                                                                                                                                                                                gtgGCGCGCGCGGCGCGTTTTATGTTACATGCGTAGGTTGAGGCAGGGTCGT‑TATGTCCGATAACCATGA‑CCTTTGCCGCCACGCCATTGTTGTTACAGATGTTACCCGCGCCGTTTCAGGACTGGACCACGCCGCTATTGAGCGATCGCTACGATTCTCACTTATTGCCAGGTGGGCAAAAACGCGGTTTGTTGATTGGCATGGGAATGACGGAAAAGCAGGGCGGTTCCGATGTTATGAGCAAcaccac                           >  1:271270/1‑251 (MQ=255)                                                                                                                                                                                                                               cgTTTTATGTTACATGCGTAGGTTGAGTCCGGGTCGT‑TATGTCCGATAAACATGA‑ACTTTGCCGCCAAGCCATTGTTGTTACAGATGTTACCCGCGCCGTTTCAGGACTGGACCACGCCGCTATTGAGCGATCGCTACGATTCTCACTTATTGCCAGGTGGGCAAAAACGCGGTTTGTTGATTGGCATGGGAATGACGGAAAAGCAGGGCGGTTCAGATGTTATGAGCAACACCACCCGTGCAGAGCGTCt            >  1:248207/1‑251 (MQ=255)                                                                                                                                                                                                                                          aCATGCGTAGGTTGAGGCAGGGTCTT‑TATGTCCGAAAACCATGC‑CCTTTGCCGCCACGCCATTGTTGTTACAGATGTTACCCGCGCCGTTTCAGGACTGGACCACGCCGCTATTGAGCGATCGCTACGATTCTCACTTATTGCCAGGTGGGCAAAAACGCGGTTTGTTGATTGGCATGGGAATGACGGAAAAGCAGGGCGGTTCCGATGTTATGAGCAACACCACCCGTGCAGAGCGTCTGGAAGATGGc  <  2:271270/250‑1 (MQ=255)                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                     ATTGGTCAGCAGTTAGGAACGGCTGAATCCCTTGAACTGGGGCGGCTGGCGAATGTGAATCCGCCTGAATTATTGCGCTACGATGCGCAAGGACGCCGTCTGGACGATGTGCGTTTTCACCCCGCCTGGCACCTGCTGATGCAGGCGCTATGTACCAATCGGGTGCACAATCTTGCCTGGGAAGAAGACGCTCGCTCCGGCGCATTTGTGGCGCGCGCGGCGCGTTTTATGTTACATGCGCAGGTTGAGGCAGGGTCGT‑TATGTCCGATAACCATGA‑CCTTTGCCGCCACGCCATTGTTGTTACAGATGTTACCCGCGCCGTTTCAGGACTGGACCACGCCGCTATTGAGCGATCGCTACGATTCTCACTTATTGCCAGGTGGGCAAAAACGCGGTTTGTTGATTGGCATGGGAATGACGGAAAAGCAGGGCGGTTCCGATGTTATGAGCAACACCACCCGTGCAGAGCGTCTGGAAGATGGC  >  NC_000913/4414410‑4414892

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑