Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	442,290	G→A	V248I (GTC→ATC)	thiI →	tRNA uridine 4‑sulfurtransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	442,290	0	G	A	100.0%	127.0 / NA	39	V248I (GTC→ATC)	thiI	tRNA uridine 4‑sulfurtransferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (15/24);  total (15/24)

ACGAAGGTATTGGCGGTTTCCCGATCGGCACCCAGGAAGATGTGCTGTCGCTCATTTCCGGTGGTTTCGACTCCGGTGTTTCCAGTTATATGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGTGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAGAAAATCGACGACGGTCAGATGGGCGTTATCCTCAAACGTATGATGGTGCGTGCCGCATCTAAAGTGGCTGAACGTTACGGCGTACAGGCGCTGGTCACCGGCGAAGCGCTCGGCCAGGTGTCCAGCCAGACGCTGACCAACCTGCGCCTGATTGATAACGTCTCCGACACGCTGATCCTGCGTCCGCTGATCTCTTACGACAAAGAGCACATCATCAACCTG  >  NC_000913/442045‑442529                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                aCGAAGGTATTGGCGGTTTCCCGATCGGCACCCAGGAAGATGTGCTGTCGCTCATTTCCGGTGGTTTCGACTCCGGTGTTTCCAGTTATATGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGTGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGtcatcg                                                                                                                                                                                                                                              <  1:41850/249‑1 (MQ=255)           tGGCGGTTTCCCGATCGGCACCCAGGAAGATGTGCTGTCGCTCATTTCCGGTGGTTTCGACTCCGGTGTTTCCAGTTATATGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGTGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAAACGGTCATCGGGGAAATTCTCg                                                                                                                                                                                                                                  >  2:209113/1‑251 (MQ=255)                 tttCCCGATCGGCACCCAGGAAGATGTGCTGTCGCTCATTTCCGGTGGTTTCGACTCCGGTGTTTCCAGTTATATGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAACTTGGCGTGCGTCAGGTGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTACTTTCGAACCGGTCATCGGGGAAATTCTCGAGnaa                                                                                                                                                                                                                             >  2:418004/1‑250 (MQ=255)                  ttCCCGATCGGCACCCAGGAAGATGTGCTGTCGCTCATTTCCGGTGGTTTCGACTACGGTGTTTCCAGTTATATGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGTGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCATCGGGGAAATTCTCGAGAAAAt                                                                                                                                                                                                                           >  1:269916/1‑251 (MQ=255)                             cACCCAGGAAGATGTGCTGTCGCTCATTTCCGGTGGTTTCGACTCCGGTGTTTCCAGTTATATGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGTGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCATCGGGGAAATTCTCGAGAAAATCGACGACGGTc                                                                                                                                                                                                                <  2:193525/251‑1 (MQ=255)                                    gAAGATGTGCTGTCGCTCATTTCCGGTGGTTTCGACTCCGGTGTTTCCAGTTATATGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGTGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCATCGGGGAAATTCTCGAGAAAATCGACGACGGTCAGATggg                                                                                                                                                                                                         <  1:129907/251‑1 (MQ=255)                                     aaGATGTGCTGTCGCTCATTTCCGGTGGTTTCGACTCCGGTGTTTCCAGTTATATGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGTGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCATCGGGGAAATTCTCGAGAAAATCGACGACGGTCAGATGGGc                                                                                                                                                                                                        <  2:423932/251‑1 (MQ=255)                                          gtgCTGTCGCTCATTTCCGGTGGTTTCGACTCAGGTGTTTCCAGTTATATGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGACATTGGCGTGCGTCAGGTGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCATCGGGGCAATTCTCGAGAAAATCGACGACGGTCAGATGGGCGTTAt                                                                                                                                                                                                   >  1:439690/1‑251 (MQ=255)                                                 cGCTCAGTGCCGGTGGTTTCGACTTCGGTGTTTCCAGTTATATGTTGATGCGTCGCGGCTGCCGCGTGCAGTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGTGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTGTGTCGCTATTAATTTCGAACCGGTCATCGGGGAAATTCTCGAGAAAATCGACGACGGTCAGATGGGCGTTATCCTCaa                                                                                                                                                                                             <  2:173752/250‑1 (MQ=255)                                                     cATTTCCGGTGGTTTCGACTCCGGTGTTTCCAGTTATATGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGTGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCATCGGGGAAATTCTCGAGAAAATCGACGACGGTCAGATGGGCGTTATCCTCAAACGt                                                                                                                                                                                         <  1:418004/250‑1 (MQ=255)                                                                                       tataTGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGTGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCATCGGGGAAATTCTCGAGAAAATCGACGACGGTCAGATGGGCGTTATCCTCAAACGTATGATGGTGCGTGCCGCATCTAAAGTGGCTGAACg                                                                                                                                                      >  1:166270/1‑251 (MQ=255)                                                                                          atGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGTGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCATCGGGGAAATTCTCGAGAAAATCGACGACGGTCAGATGGGCGTTATCCTCAAACGTATGATGGTGCGTGCCGCATCTAAAGTGGCTGAACGtt                                                                                                                                                    >  1:77411/1‑250 (MQ=255)                                                                                            gTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGTGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCATCGGGGAAATTCTCGAGAAAATCGACGACGGTCAGATGGGCGTTATCCTCAAACGTATGATGGTGCGTGCCGCATCTAAAGTGGCTGAACGTTAc                                                                                                                                                  <  2:77411/250‑1 (MQ=255)                                                                                                    gTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGTGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCATCGGGGAAATTCTCGAGAAAATCGACGACGGTCAGATGGGCGTTATCCTCAAACGTATGATGGTGCGTGCCGCATCTAAAGTGGCTGAACGTTACGGCGTACAg                                                                                                                                         <  1:209113/251‑1 (MQ=255)                                                                                                         ggCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGTGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCATCGGGGAAATTCTCGAGAAAATCGACGACGGTCAGATGGGCGTTATCCTCAAACGTATGATGGTGCGTGCCGCATCTAAAGTGGCTGAACGTTACGGCGTACAGGCGCt                                                                                                                                    <  2:250609/251‑1 (MQ=255)                                                                                                             gCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGTGTCAGGTGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCATCGGGGAAATTCTCGAGAAAATCGACGACGGTCAGATGGGCGTTATCCTCAAACGTATGATGGTGCGTGCCGCATCTAAAGTGGCTGAACGTTACGGCGTACAGGCGCTGGTc                                                                                                                                >  2:380122/1‑251 (MQ=255)                                                                                                                 cgTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGTGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCATCGGGGAAATTCTCGAGAAAATCGACGACGGTCAGATGGGCGTTATCCTCAAACGTATGATGGTGCGTGCCGCATCTAAAGTGGCTGAACGTTACGGCGTACAGGCGCTGGTCACCg                                                                                                                            <  1:20966/251‑1 (MQ=255)                                                                                                                  gTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGTGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCC‑ACCGCGTGCGTTTTGTCGCTATTAATTTCGAAACGGTCATTGGGGAAATTCTTGAGGAAATCGACGACGGGCAGATGGGCGTTATTCTCAAACGTATGATGGTGCGTGCCGCATCTTAAGTGGCTGGACGTTACGGCGTACAGgcgccgggcaacggc                                                                                                                          >  2:172871/1‑240 (MQ=255)                                                                                                                    gCATTACTGCTTCTTTAACCGCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGTGGCGCCGTATCTGTGGAACCGCTTTGGCAGCTCACACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCATCGGGGAAATTCTCGAGAAAATCGACGACGGTCAGATGGGCGTTATCCTCAAACGTATGATGGTGCGTGCCGCATCTAAAGTGGCTGAACGTTACGGCGTACAGGCGCTGGTCACCGGCg                                                                                                                         <  2:330551/251‑1 (MQ=255)                                                                                                                        tACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGTGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCATCGGGGAAATTCTCGAGAAAATCGACGACGGTCAGATGGGCGTTATCCTCAAACGTATGATGGTGCGTGCCGCATCTACAGTGGCTGAACGTTACGGCGTACAGGCGCGGGTCACCGGCGAAgc                                                                                                                     >  2:1024/1‑251 (MQ=255)                                                                                                                          cTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGTGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCATCGGGGAAATTCTCGAGAAAATCGACGACGGTCAGATGGGCGTTATCCTCAAACGTATGATGGTGCGTGCCGCATCTAAAGTGGCTGAACGTTACGGCGTACAGGCGCTGGTCACCGGCGAAgcgc                                                                                                                   >  2:173439/1‑251 (MQ=255)                                                                                                                               tcttTAACCTCGGCGGCGCGGCGCATGACATTGGCGTGCGTCAGGTGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGGGCGTTTTGTCGCTATTAATTTCGAACCGGTCATCGGGGAAATTCTCGAGAAAATCGACGACGGTCAGATGGGCGTTATCCTCAAACGTATGATGGTGCGTGCCGCATCTAAAGTGGCTGAACGTTACGGCGTACAGGCGCTGGTCAACGGCGAAGCGCTCGGc                                                                                                              >  2:44040/1‑251 (MQ=255)                                                                                                                                         cggcggCGCGGCGCATGAAATTGGCGTGCGTCAGGTGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCATCGGGGAAATTCTCGAGAAAATCGACGACGGTCAGATGGGCGTTATCCTCAAACGTATGATGGTGCGTGCCGCATCTAAAGTGGCTGAACGTTACGGCGTACAGGCGCTGGTCACCGGCGAAGCGCTCGGCCAGGTGTc                                                                                                      <  1:307907/249‑1 (MQ=255)                                                                                                                                                  ggcgcATGAAATTGGCGTGCGTCAGGTGGCGCATTATCTGTGGAACCGATTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCATCGGGGAAATTCTCGAGAAAATCGACGACGGTCAGATGGGCGTTATCCTCAAACGTATGATGGTGCGTGCCGCATCTAAAGTGGCTGAACGTTAAGGCGTACAGGCGCTGGTCACCGGCGAAGCGCTCGGCCAGGTGTCCAGCCAGACGc                                                                                           >  1:20377/1‑251 (MQ=255)                                                                                                                                                       aTGAAATTGGCGTGCGTGAGGTGGCGCATTATCTGTGGAACCGCTTTGGCAGCTACAACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCATCGGGGAAATTCTCGAGAAAATCGACGACGGTCAGATGGGCGTTATCCTCAAACGTATGATGGTGCGTGCCGCATCTAAAGTGGCTGAACGTTACGGCGTACAGGCGCTGGTCACCGGCGAAGCGCTCGGCCAGGTGTCCAGACAGACGCTGAcc                                                                                      >  2:359034/1‑251 (MQ=255)                                                                                                                                                         gAAATTGGCGTGCGTAAGGTGGCGCATTATCGGTGGACCCGCTTTGGCAGCTCC‑ACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCATCGGGGAAATTCTCGAGAAAATCGACGACGGTCAGATGGGCGTTATCCTCAAACGTATGATGGTGCGTGCCGCATCTAAAGTGGCTGAACGTTACGGCGTACAGGCGCTGGTCACCGGCGAAGCGCTCGGCCAGGTGTCCAGCCAGACGCTGACCaa                                                                                    <  1:172871/250‑1 (MQ=255)                                                                                                                                                           aaTTGGCGTGCGTCAGGTGGCGCATTATCTGTGGATCCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCATCGGGGAAATTCTCGAGAAAATCGACGACGGTCAGATGGGCGTTATCCTCAAACGTATGATGGTGCGTGCCGCATCTAAAGTGGCTGAACGTTACGGCGTACAGGCGCTGGTCACCGGCGAAGCGCTCGGCCAGGTGTCCAGCCAGACGCTGACCAAcc                                                                                  <  1:313030/251‑1 (MQ=255)                                                                                                                                                                  gtgcgtCAGGTGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCATCGGGGAAATTCTCGAGAAAATCGACGACGGTCAGATGGGCGTTATCCTCAAACGTATGATGGTGCGTGCCGCATCTAAAGTGGCTGAACGTTACGGCGTACAGGCGCTGGTCACAGGCGAAGCGCTCGGCCAGGTGTCCAGCCAGACGCTGACCAACCTGCGCCt                                                                           >  2:308079/1‑251 (MQ=255)                                                                                                                                                                                                 gCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCATCGGGGAAATTCTCGAGAAAATCGACGACGGTCAGATGGGCGTTATCCTCAAACGTATGATGGTGCGTGCCGCATCTAAAGTGGCTGAACGTTACGGCGTACAGGCGCTGGTCACCGGCGAAGCGCTCGGCCAGGTGTCCAGCCAGACGCTGACCAACCTGCGCCTGATTGATAACGTCTCCGACACGCTGATCCTg                                            <  1:298799/251‑1 (MQ=255)                                                                                                                                                                                                 gCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCATCGGGGAAATTCTCGAGAAAATCGACGACGGTCAGATGGGCGTTATCCTCAAACGTATGATGGTGCGTGCCGCATCTAAAGTGGCTGAACGTTACGGCGTACAGGCGCTGGTCACCGGCGAAGCGCTCGGCCAGGTGTCCAGCCAGACGCTGACCAACCTGCGCCTGATTGATAACGTCTCCGACACGCTGATCCTg                                            <  1:424429/251‑1 (MQ=255)                                                                                                                                                                                                      ggCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCATCGGGGAAATTCTCGAGAAAATCGACGACGGTCAGATGGGCGTTATCCTCAAACGTATGATGGTGCGTGCCGCATCTAAAGTGGCTGAACGTTACGGCGTACAGGCGCTGGTCACCGGCGAAGCGCTCGGCCAGGTGTCCAGCCAGACGCTGACCAACCTGCGCCTGATTGATAACGTCTCCGACACGCTGATCCTGCGTcc                                       <  1:417033/251‑1 (MQ=255)                                                                                                                                                                                                             cccACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCATCGGGGAAATTCTCGAGAAAATCGACGACGGTCAGATGGGCGTTATCCTCAAACGTATGATGGTGCGTGCCGCATCTAAAGTGGCTGAACGTTACGGCGTACAGGCGCTGGTCACCGGCGAAGCGCTCGGCCAGGTGTCCAGCCAGACGCTGACCAACCTGCGCCTGATTGATAACGTCTCCGACACGCTGATCCTGCGTCCGCTGAtc                                <  1:128726/251‑1 (MQ=255)                                                                                                                                                                                                                  cgcgTGCGTTTTTGCGGTATTAATTTCGAACCGGTCAGCGTGGAAATTCTCGAGAAAATCGACGACGGTCAGATGGGCGTTATCCTCAAACGTATGATGGTGCGGGCCGCATCTAAAGTGGCTGAACGTTACGGCGTACAGGCGCGGGTGACCGGCGAAGCGCTCGGCCAGGTGTCCAGCCAGACGCTGACCAACCTGCGCCGGGTTGATAACGTCTCCGACGCGCTGATCCTGCGTCCGCTGATCTCTTa                           <  2:439690/251‑1 (MQ=255)                                                                                                                                                                                                                   gagtgcgtTTTGTCGCTATTAATTTCGAACCGGTCATCGGGGAAATTCTCGAGAAAATCGACGACGGTCAGATGGGCGTTATCCTCAAACGTATGATGGTGCGTGCCGCATCTAAAGTGGCTGAACGTTACGGCGTACAGGCGCTGGTCACCGGCGAAGCGCTCGGCCAGGTGTCCAGCCAGACGCTGACCAACCTGCGCCTGATTGATAACGTCTCCGACACGCTGATCCTGCGTCCGCTGATCTCTTAc                          <  2:158079/249‑1 (MQ=255)                                                                                                                                                                                                                         gtTTTGTCGCTATTAATTTCGAACCGGTCATCGGGGAAATTCTCGAGAAAATCGACGACGGTCAGATGGGCGTTATCCTCAAACGTATGATGGTGCGTGCCGCATCTAAAGTGGCTGAACGTTACGGCGTACAGGCGCTGGTCACCGGCGAAGCGCTCGGCCAGGTGTCCAGCCAGACGCTGACCAACCTGCGCCTGATTGATAACGTCTCCGACACGCTGATCCTGCGTCCGCTGATCTCTTACGACaaa                    <  2:229058/251‑1 (MQ=255)                                                                                                                                                                                                                            ttGTCGCTATTAAGTTCGAACCGGTCATCGGGGAAATTCTCGAGAAAATCGACGACGGGCAGATGGGCGTTATCCTCAAACGTATGATGGTGCGTGCCGCATCTAAAGTGGTTGAACGGTACGGCGTACAGGCGCGGGTCACCGGCGAAGCGCTCGGCCAGGTGTCCAGCCAGAGGCTGACCAACCTGCGCCTGATTGAGAACGTCTCCGACACGCTGATCCTGCGTCCGCTGATCTCTTACGACAAaga                  <  1:56823/250‑1 (MQ=255)                                                                                                                                                                                                                                       aaTTTCGAACCGGTCATCGGGGAAATTCTCGAGAAAATCGACGACGGTCAGATGGGCGTTATCCTCAAACGTATGATGGTGCGTGCCGCATCTAAAGTGGCTGAACGTTACGGCGTACAGGCGCTGGTCACCGGCGAAGCGCTCGGCCAGGTGTCCAGCCAGACGCTGACCAACCTGCGCCTGATTGATAACGTCTCCGACACGCTGATCCTGCGTCCGCTGATCTCTTACGACAAAGAGCACATCATCaa      <  2:103387/251‑1 (MQ=255)                                                                                                                                                                                                                                       aaTTTCGAACCGGTCATCGGGGAAATTATCGACAACATCGACGACGGTCAGATGGGCGTTATCCTCAAACGTATGATGGTGCGTGCCGCATCTAAAGTGGCTGAACGTTACGGCGTACAGGCGCTGGTCACCGGCGAAGCGCTCGGCCAGGTGTCCAGCCAGACGCTGACCAACCTGCGCCTGATTGATAACGTCTCCGACACGCTGATCCTGCGTCCGCTGATCTCTTACGACAAAGAGCACAtcatca       >  1:384918/1‑250 (MQ=255)                                                                                                                                                                                                                                          ttCGAACCTGTCATCGGGGAAATTCTCGAGAAAATCGACGACGGTCAGATGGGCGTTATCCTCAAACGTATGATGGTGCGTGCCGCATCTAAAGTGGCTGAACGTTACGGCGTACAGGCGCTGGTCACCGGCGAAGCGCTCGGCCAGGTGTCCAGCCAGACGCTGACCAACCTGCGCCTGATTGATAACGTCTCCGACACGCTGATCCTGCGTCCGCTGATCTCTTACGACAAAGAGCACATCATCAAcc    <  2:299366/250‑1 (MQ=255)                                                                                                                                                                                                                                            cGAACCGGTCATCGGGGAAATTCTCGAGAAAATCGACGACGGTCAGATGGGCGTTATCCTCAAACGTATGATGGTGCGTGCCGCATCTAAAGTGGCTGAACGTTACGGCGTACAGGCGCTGGTCACCGGCGAAGCGCTCGGCCAGGTGTCCAGCCAGACGCTGACCAACCTGCGCCTGATTGATAACGTCTCCGACACGCTGATCCTGCGTCCGCTGATCTCTTACGACAAAGAGCACATCATCAACCTg  <  1:173439/250‑1 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                ACGAAGGTATTGGCGGTTTCCCGATCGGCACCCAGGAAGATGTGCTGTCGCTCATTTCCGGTGGTTTCGACTCCGGTGTTTCCAGTTATATGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGTGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAGAAAATCGACGACGGTCAGATGGGCGTTATCCTCAAACGTATGATGGTGCGTGCCGCATCTAAAGTGGCTGAACGTTACGGCGTACAGGCGCTGGTCACCGGCGAAGCGCTCGGCCAGGTGTCCAGCCAGACGCTGACCAACCTGCGCCTGATTGATAACGTCTCCGACACGCTGATCCTGCGTCCGCTGATCTCTTACGACAAAGAGCACATCATCAACCTG  >  NC_000913/442045‑442529

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑