Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	495,221	G→A	E34E (GAG→GAA)	htpG →	chaperone protein HtpG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	495,221	0	G	A	100.0%	199.1 / NA	59	E34E (GAG→GAA)	htpG	chaperone protein HtpG
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (28/31);  total (28/31)

CCTTGCCGGGCGTCATAAGATTCGTTTTTAAGCAAACGAGAGCAGGATCACCTGCTCTCGCTTGAAATTATTCTCCCTTGTCCCCATCTCTCCCACATCCTGTTTTTAACCTTAAAATGGCATTATTGAGGTAGACCTACATGAAAGGACAAGAAACTCGTGGTTTTCAGTCAGAAGTGAAACAGCTTCTGCACCTGATGATCCATTCTCTCTATTCCAATAAAGAAATCTTCCTGCGTGAGCTTATCTCTAACGCCTCCGATGCGGCGGACAAGCTGCGTTTCCGTGCGCTCTCTAACCCGGACCTGTACGAAGGTGATGGCGAACTACGCGTTCGTGTCTCTTTCGATAAAGACAAGCGTACGCTGACCATCTCCGATAACGGCGTGGGGATGACCCGCGACGAAGTGATTGACCATCTGGGGACTATCGCTAAATCCGGTACCAAATCATTCCTCGAATCCCTGGGTTCTGACCAGGCGAAAGAC  >  NC_000913/494980‑495467                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                       ccTTGCCGGGCGTCATAAGATTCGTTTTTAAGCAAACGAGAGCAGGATCACCTGCTCTCGCTTGAAATTATTCTCCCTTGTCCCCATCTCTCCCACATCCTGTTTTTAACCTTAAAATGGCATTATTGAGGTAGACCTACATGAAAGGACAAGAAACTCGTGGTTTTCAGTCAGAAGTGAAACAGCTTCTGCACCTGATGATCCATTCTCTCTATTCCAATAAAGAAATCTTCCTGCGTGAACTTATctct                                                                                                                                                                                                                                               <  2:166215/251‑1 (MQ=255)        gggCGTCATAAGATTAGTTTTTAAGCAAACGAGAGCAGGATCACCTGCTCTCGCTTGAAATTATTCTCCCTTGTCCCCATCTCTCCCACATCCTGTTTTTAACCTTAAAATGGCATTATTGAGGTAGACCTACATGCAAGGACAAGAAACTCGTGGTTTTCAGTCAGAAGTGAAACAGCTTCTGCACCTGATGATCCATTCTCTCTATTCCAATAAAGAAATCTTCCTGCGTGAACTTATCTCTAACGCCt                                                                                                                                                                                                                                        >  1:377747/1‑251 (MQ=255)                 aaGATTCGTTTTTAAGCAAACGAGAGCAGGATCACCTGCTCTCGCTTGAAATTATTCTCCCTTGTCCCCATCTCTCCCACATCCTGTTTTTAACCTTAAAATGGCATTATTGAGGTAGACCTACATGAAAGGACAAGAAACTCGTGGTTTTCAGTCAGAAGTGAAACAGCTTCTGCACCTGATGATCCATTCTCTCTATTCCAATAAAGAAATCTTCCTGCGTGAACTTATCTCTAACGCCTCCGATgcgg                                                                                                                                                                                                                               >  1:101335/1‑251 (MQ=255)                    aTTCGTTTTTAAGCAAACGAGAGCAGGATCACCTGCTCTCGCTTGAAATTATTCTCCCTTGTCCCCATCTCTCCCACATCCTGTTTTTAACCTTAAAATGGCATTATTGAGGTAGACCTACATGAAAGGACAAGAAACTCGTGGTTTTCAGTCAGAAGTGAAACAGCTTCTGCACCTGATGATCCATTCTCTCTATTCCAATAAAGAAATCTTCCTGCGTGAACTTATCTCTAACGCCTCCGATGcggcgg                                                                                                                                                                                                                            <  1:298412/251‑1 (MQ=255)                        gTTTTTAAGCAAACGAGAGCAGGATCACCTGCTCTCGCTTGAAATTATTCTCCCTTGTCCCCATCTCTCCCACATCCTGTTTTTAACCTTAAAATGGCATTATTGAGGTAGACCTACATGAAAGGACAAGAAACTCGTGGTTTTCAGTCAGAAGTGAAACAGCTTCTGCACCTGATGATCCATTCTCTCTATTCCAATAAAGAAATCTTCCTGCGTGAACTTATCTCTAACGCCTCCGATGCGCCGGACaa                                                                                                                                                                                                                        <  2:210297/251‑1 (MQ=255)                          ttttAAGCAAACGAGAGCAGGATCACCTGCTCTCGCTTGAAATTATTCTCCCTTATCCCCATCTCTCCCACATCCTGTTTTTAAACTTAAAATGGCATTATTGAGGTAGACCTACATGAACGGACAAGAAACTCGTGGTTTTCAGTCAGAAGTGAAACAGCTTCTGCACCTGATGATCCATTCTCTCTATTCCAATAAAGAAATCTTCCTGCGTGAACTTATCTCTAACGCCTCCGATGCGGCGGACAAGc                                                                                                                                                                                                                      >  1:203223/1‑251 (MQ=255)                             tAAGCAAACGAGAGCAGGATCACCTGCTCTCGCTTGAAATTATTCTCCCTTGTCCCCATCTCTCCCACATCCTGTTTTTAACCTTAAAATGGCATTATTGAGGTAGACCTACATGAAAGGACAAGAAACTCGTGGTTTTCAGTCAGAAGTGAAACAGCTTCTGCACCTGATGATCCATTCTCTCTATTCCAATAAAGAAATCTTCCTGCGTGAACTTATCTCTAACGCCTCCGATGCGGCGGCCAAGCTGc                                                                                                                                                                                                                   >  1:341491/1‑251 (MQ=255)                              aaGCAAACGAGAGCAGTCTCACCTGCTCTCGCTTGAAATTATTCTCCCTTGTCCCCATCTCTCCCACATCCTGTTTTTAACCTTAAAATGGCATTATTGAGGTAGACCTACATGAAAGGACAAGAAACTCGTGGTTTTCAGTCAGAAGTGAAACAGCTTCTGCACCTGATGATCCATTCTCTCTATTCCAATAAAGAAATCTTCCTGCGTGAACTTATCTCTAACGCCTCCGATGCGGCGGACAAGCt                                                                                                                                                                                                                     <  2:328418/248‑1 (MQ=255)                                 cAAACGAGAGCAGGATCACCTGCTCTCGCTTGAAATTATTCTCCCTTGTCCCCATCTCTCCCACATCCTGTTTTTAACCTTAAAATGGCATTATTGAGGTAGACCTACATGAAAGGACAAGAAACTCGTGGTTTTCAGTCAGAAGTGAAACAGCTTCTGCACCTGATGATCCATTCTCTCTATTCCAATAAAGAAATCTTCCTGCGTGAACTTATCTCTAACGCCTCCGATGCGGCGGACAAGCTGCGttt                                                                                                                                                                                                               <  2:101335/251‑1 (MQ=255)                                    aCGAGAGCAGGATCACCTGCTCTCGCTTGAAATTATTCTCCCTTGTCCCCATCTCTCCCACATCCTGTTTTTAACCTTAAAATGGCATTATTGAGGTAGACCTACATGAAAGGACAAGAAACTCGTGGTTTTCAGTCAGAAGTGAAACAGCTTCTGCACCTGATGATCCATTCTCTCTATTCCAATAAAGAAATCTTCCTGCGTGAACTTATCTCTAACGCCTCCGATGCGGCGGACAAGCTGCGTTTCCg                                                                                                                                                                                                            <  1:324134/251‑1 (MQ=255)                                    aCGAGAGCAGGATCACCTGCTCTCGCTTGAAATTATTCTCCCTTGTCCCCATCTCTCCCACATCCTGTTTTTAACCTTAAAATGGCATTATTGAGGTAGACCTACATGAAAGGACAAGAAACTCGTGGTTTTCAGTCAGAAGTGAAACAGCTTCTGCACCTGATGATCCATTCTCTCTATTCCAATAAAGAAATCTTCCTGCGTGAACTTATCTCTAACGCCTCCGATGCGGCGGACAAGCTGCGTTTCCg                                                                                                                                                                                                            >  2:435559/1‑251 (MQ=255)                                           cAGGATCACCTGCTCTCGCTTGAAATTATTCTCCCTTGTCCCCATCTCTCCCACATCCTGTTTTTAACCTTAAAATGGCATTATTGAGGTAGACCTACATGAAAGGACAAGAAACTCGTGGTTTTCAGTCAGAAGTGAAACAGCTTCTGCACCTGATGATCCATTCTCTCTATTCCAATAAAGAAATCTTCCTGCGTGAACTTATCTCTAACGCCTCCGATGCGGCGGACAAGCTGCGTTTCCGTGCGctc                                                                                                                                                                                                     <  1:133094/251‑1 (MQ=255)                                           cAGGATCACCTGCTCTCGCTTGAAATTATTCTCCCTTGTCCCCATCTCTCCCACATCCTGTTTTTAACCTTAAAATGGCATTATTGAGGTAGACCTACATGAAAGGACAAGAAACTCGTGGTTTTCAGTCAGAAGTGAAACAGCTTCTGCACCTGATGATCCATTCTCTCTATTCCAATAAAGAAATCTTCCTGCGTGAACTTATCTCTAACGCCTCCGATGCGGCGGACAAGCTGCGTTTCCGTGCGctc                                                                                                                                                                                                     >  1:397918/1‑251 (MQ=255)                                                                gAAATTATTCTCCCTTGTCCCCATCTCTCCCACATCCTGTTTTTAACCTTAAAATGGCATTATTGAGGTAGACCTACATGAAAGGACAAGAAACTCGTGGTTTTCAGTCAGAAGTGAAACAGCTTCTGCACCTGATGATCCATTCTCTCTATTCCAATAAAGAAATCTTCCTGCGTGAACTTATCTCTAACGCCTCCGATGCGGCGGACAAGCTGCGTTTCCGTGCGCTCTCTAACCCGGACCTGTACGaa                                                                                                                                                                                <  2:204431/251‑1 (MQ=255)                                                                 aaaTTATTCTCCCTTGTCCCCATCTCTCCCACATCCTGTTTTTAACCTTAAAATGGCATTATTGAGGTAGACCTACATGAAAGGACAAGAAACTCGTGGTTTTCAGTCAGAAGTGAAACAGCTTCTGCACCTGATGATCCATTCTCTCTATTCCAATAAAGAAATCTTCCTGCGTGAACTTATCTCTAACGCCTCCGATGCGGCGGACAAGCTGCGTTTCCGTGCGCTCTCTAACCCGGACCTGTACGa                                                                                                                                                                                 >  2:225656/1‑249 (MQ=255)                                                                 aaaTTATTCTCCCTTGTCCCCATCTCTCCCACATCCTGTTTTTAACCTTAAAATGGCATTATTGAGGTAGACCTACATGAAAGGACAAGAAACTCGTGGTTTTCAGTCAGAAGTGAAACAGCTTCTGCACCTGATGATCCATTCTCTCTATTCCAATAAAGAAATCTTCCTGCGTGAACTTATCTCTAACGCCTCCGATGCGGCGGACAAGCTGCGTTTCCGTGCGCTCTCTAACCCGGACCTGTACGAAg                                                                                                                                                                               <  2:164260/251‑1 (MQ=255)                                                                          tcCCTTGTCCCCATCTCTCCCACATCCTGTTTTTAACCTTAAAATGGCATTATTGAGGTAGACCTACATGAAAGGACAAGAAACTCGTGGTTTTCAGTCAGAAGTGAAACAGCTTCTGCACCTGATGATCCATTCTCTCTATTCCAATAAAGAAATCTTCCTGCGTGAACTTATCTCTAACGCCTCCGATGCGGCGGACAAGCTGCGTTTCCGTGCGCTCTCTAACCCGGACCTGTACGAAGGCGATGGCg                                                                                                                                                                      >  1:102087/1‑251 (MQ=255)                                                                               tGTCCCCATCTCTCCCACATCCTGTTTTTCACCTTAAAATGGCATTATTGCGGTAGACCTACATGAAAGGACAAGAAACTCGTGGTTTTCAGTCAGAAGTGAAACAGCTTCTGCACCTGATGATCCATTCTCTCTATTCCAATAAAGAAATCTTCCTGCGTGAACTTATCTCTACCGCCTCCGATGCGGCGGACAAGCTGCGTTTCCGTGCGCTCTCTAACCCGGACCTGTACGAAGGTGATGGCGAACt                                                                                                                                                                  >  2:52651/1‑250 (MQ=255)                                                                                             ccACATCCTGTTTTTAACCTTAAAATGGCATTATTGAGGTAGACCTACATGAAAGGACAAGAAACTCGTGGTTTTCAGTCAGAAGTGAAACAGCTTCTGCACCTGATGATCCATTCTCTCTATTCCAATAAAGAAATCTTCCTGCGTGAACTTATCTCTAACGCCTCCGATGCGGCGGACAAGCTGCGTTTCCGTGCGCTCTGTACCCCGGACCTGTACGAAGGTGATGGCGAACTACGCGTTCGTGtctc                                                                                                                                                   <  1:427649/251‑1 (MQ=255)                                                                                                 aTCCTGTTTTTAACCTTAAAATGGCATTATTGAGGTAGACCTACATGAAAGGACAAGAAACTCGTGGTTTTCAGTCAGAAGTGAAACAGCTTCTGCACCTGATGATCCATTCTCTCTATTCCAATAAAGAAATCTTCCTGCGTGAACTTATCTCTAACGCCTCCGATGCGGCGGACAAGCTGCGTTTCCGTGCGCTCTCTAACCCGGACCTGTACGAAGGTGATGGCGAACTACGCGTTCGTGTCTCTTTc                                                                                                                                               <  1:272006/251‑1 (MQ=255)                                                                                                   ccTGTTTTTAACCTTAAAATGGCATTATTGAGGTAGACCTACATGAAAGGACAAGAAACTCGTGGTTTTCAGTCAGAAGTGAAACAGCTTCTGCACCTGATGATCCATTCTCTCTATTCCAATAAAGAAATCTTCCTGCGTGAACTTATCTCTAACGCCTCCGATGCGGCGGACAAGCTGCGTTTCCGTGCGCTCTCTAACCCGGACCTGTACGAAGGTGATGGCGAACTACGCGTTCGTGTCTCTTTCGa                                                                                                                                             <  1:435559/251‑1 (MQ=255)                                                                                                                 tAAAATGGCATTATTGAGGTAGACCTACATGAAAGGACAAGAAACTCGTGGTTTTCAGTCAGAAGTGAAACAGCTTCTGCACCTGATGATCCATTCTCTCTATTCCAATAAAGAAATCTTCCTGCGTGAACTTATCTCTAACGCCTCCGATGCGGCGGACAAGCTGCGTTTCCGTGCGCTCTCTAACCCGGACCTGTACGAAGGTGATGGCGAACTACGCGTTCGTGTCTCTTTCGATAAAGACAAGCg                                                                                                                                 <  1:335048/249‑1 (MQ=255)                                                                                                                    aaTGGCATTATTGAGGTAGACCTACATGAAAGGACAAGAAACTCGTGGTTTTCAGTCAGAAGTGAAACAGCTTCTGCACCTGATGATCCATTCTCTCTATTCCAATAAAGAAATCTTCCTGCGTGAACTTATCTCTAACGCCTCCGATGCGGCGGACAAGCTGCGTTTCCGTGCGCTCTCTAACCCGGACCTGTACGAAGGTGATGGCGAACTACGCGTTCGTGTCTCTTTCGATAAAGACAAGCGTACGc                                                                                                                            >  1:101720/1‑251 (MQ=255)                                                                                                                     aTGGCCTTATTGAGGTAGACCTACATGAAAGGACAAGAACCTCGTGGTTTTCAGTCAGAAGTGAAACAGCTTCTGCACCTGATGATCCATTCTCTCTATTCCAATAAAGAAATCTTCCTGCGTGAACTTATCTCTAACGCCTCCGATGCGGCGGACAAGCTGCGTTTCCGTGCGCTCTCTAACCCGGACCTGTACGAAGGTGATGGCGAACTACGCGTTCGTGTCTCTTTCGATAAAGa                                                                                                                                       >  2:454178/1‑239 (MQ=255)                                                                                                                     aTGGCATTATTGAGGTAGACCTACATGAAAGGACAAGAAACTCGTGGTTTTCAGTCAGAAGTGAAACAGCTTCTGCACCTGATGATCCATTCTCTCTATTCCAATAAAGAAATCTTCCTGCGTGAACTTATCTCTAACGCCTCCGATGCGGCGGACAAGCTGCGTTTCCGTGCGCTCTCTAACCCGGACCTGTACGAAGGTGATGGCGAACTACGCGTTCGTGTCTCTTTCGATAAAGa                                                                                                                                       <  1:454178/239‑1 (MQ=255)                                                                                                                         cATTATTGAGGTAGACCTACATGAAAGGACAAGAAACTCGTGGTTTTCAGTCAGAAGTGAAACAGCTTCTGCACCTGATGATCCATTCTCTCTATTCCAATAAAGAAATCTTCCTGCGTGAACTTATCTCTAACGCCTCCGATGCGGCGGACAAGCTGCGTTTCCGTGCGCTCTCTAACCCGGACCTGTACGAAGGTGATGGCGAACTACGCGTTCGTGTCTCTTTCGATAAAGACAAGCGTACGCTGAcc                                                                                                                       <  2:112681/251‑1 (MQ=255)                                                                                                                         cATTATTGAGGTAGACCTACATGAAAGGACAAGAAACTCGTGGTTTTCAGTCAGAAGTGAAACAGCTTCTGCACCTGATGATCCATTCTCTCTATTCCAATAAAGAAATCTTCCTGCGTGAACTTATCTCTAACGCCTCCGATGCGGCGGACAAGCTGCGTTTCCGTGCGCTCTCTAACCCGGACCTGTACGAAGGTGATGGCGAACTACGCGTTCGTGTCTCTTTCGATAAAGACAAGCGTACGCTGAcc                                                                                                                       >  1:375685/1‑251 (MQ=255)                                                                                                                           ttattGAGGTAGACCTACATGAAAGGACAAGAAACTCGTGGTTTTCAGTCAGAAGTGAAACAGCTTCTGCACCTGATGATCCATTCTCTCTATTCCAATAAAGAAATCTTCCTGCGTGAACTTATCTCTAACGCCTCCGATGCGGCGGACAAGCTGCGTTTCCGTGCGCTCTCTAACCCGGACCTGTACGAAGGTGATGGCGAACTACGCGTTCGTGTCTCTTTCGATAAAGACAAGCGTACGCTGACCAt                                                                                                                     >  1:438313/1‑251 (MQ=255)                                                                                                                            tattGAGGTAGACCTACATGAAAGGACAAGAAACTCGTGGTTTTCAGTCAGAAGTGAAACAGCTTCTGCACCTGATGATCCATTCTCTCTATTCCAATAAAGAAATCTTCCTGCGTGAACTTATCTCTAACGCCTCCGATGCGGCGGACAAGCTGCGTTTCCGTGCGCTCTCTAACCCGGACCTGTACGAAGGTGATGGCGAACTACGCGTTCGTGTCTCTTTCGATAAAGACAAGCGTACGCTGACCAtc                                                                                                                    >  1:31442/1‑251 (MQ=255)                                                                                                                                gAGGTAGACCTACATGAAAGGACAAGAAACTCGTGGTTTTCAGTCAGAAGTGAAACAGCTTCTGCACCTGATGATCCATTCTCTCTATTCCAATAAAGAAATCTTCCTGCGTGAACTTATCTCTAACGCCTCCGATGCGGCGGACAAGCTGCGTTTCCGTGCGCTCTCTAACCCGGACCTGTACGAAGGTGATGGCGAACTACGCGTTCGTGTCTCTTTCGATAAAGACAAGCGTACGCTGACCATCTCCg                                                                                                                <  1:437712/251‑1 (MQ=255)                                                                                                                                gAGGTAGACCTACATGAAAGGACAAGAAACTCGTGGTTTTCAGTCAGAAGTGAAACAGCTTCTGCACCTGATGATCCATTCTCTCTATTCAAATAAAGAAATCTTCCTGCGTGAACTTATCTCTAACGCCTCCGATGCGGCGGACAAGCTGCGTTTCCGTGCGCTCTCTAACCCGGACCTGTACGAAGGTGATGGCGAACTACGCGTTCGTGTCTCTTTCGATAAAGACAAGCGTACGCTGACCATCTCCg                                                                                                                >  2:340743/1‑251 (MQ=255)                                                                                                                                     aGACCTACATGAAAGGACAAGAAACTCGTGGTTTTCAGTCAGAAGTGAAACAGCTTCTGCACCTGATGATCCATTCTCTCTATTCCAATAAAGAAATCTTCCTGCGTGAACTTATCTCTAACGCCTCCGATGCGGCGGACAAGCTGCGTTTCCGTGCGCTCTCTAACCCGGACCTGTACGAAGGTGATGGCGAACTACGCGTTCGTGTCTCTTTCGATAAAGACAAGCGTACGCTGACCATCTCCGATAAc                                                                                                           <  1:52651/251‑1 (MQ=255)                                                                                                                                                 aaGGACAAGAAACTCGTGGTTTTCAGTCAGAAGTGAAACAGCTTCTGCACCTGATGATCCATTCTCTCTATTCCAATAAAGAAATCTTCCTGCGTGAACTTATCTCTAACGCCTCCGATGCGGCGGACAAGCTGCGTTTCCGTGCGCTCTCTAACCCGGACCTGTACGAAGGTGATGGCGAACTACGCGTTCGTGTCTCTTTCGATAAAGACAAGCGTACGCTGACCATCTCGGATAACGGCGTTGGGATg                                                                                               >  2:353317/1‑251 (MQ=255)                                                                                                                                                 aaGGACAAGAAACTCGTGGTTTTCAGTCAGAAGTGAAACAGCTTCTGCACCTGATGATCCATTCTCTCTATTCCAATAAAGAAATCTTCCTGCGTGAACTTATCTCTAACGCCTCCGATGCGGCGGACAAGCTGCGTTTCCGTGCGCTCTCTAACCCGGACCTGTACGAAGGTGATGGCGAACTACGCGTTCGTGTCTCTTTCGATAAAGACAAGCGTACGCTGACCATCTCCGATAACGGCGTgggggtg                                                                                               >  2:88282/1‑248 (MQ=255)                                                                                                                                                    gACAAGAAACTCGTGGTTTTCAGTCAGAAGTGAAACAGCTTCTGCACCTGATGATCCATTCTCTCTATTCCAATAAAGAAATCTTCCTGCGTGAACTTATCTCTAACGCCTCCGATGCGGCGGACAAGCTGCGTTTCCGTGCGCTCTCTAACCCGGACCTGTACGAAGGTGATGGCGAACTACGCGTTCGTGTCTCTTTCGATAAAGACAAGCGTACGCTGACCATCTCCGATAACGGCGTGGGGATGAcc                                                                                            >  1:164943/1‑251 (MQ=255)                                                                                                                                                      cAAGAAACTCGTGGTTTTCAGTCAGAAGTGAAACAGCTTCTGCACCTGATGATCCATTCTCTCTATTCCAATAAAGAAATCTTCCTGCGTGAACTTATCTCTAACGCCTCCGATGCGGCGGACAAGCTGCGTTTCCGTGCGCTCTCTAACCCGGACCTGTACGAAGGTGATGGCGAACTACGCGTTCGTGTCTCTTTCGATAAAGACAAGCGTACGCTGACCATCTCCGATAACGGCGTGGGGATGACCcg                                                                                          >  1:161393/1‑251 (MQ=255)                                                                                                                                                        aGAAACTCGTGGTTTGCAGTCAGAAGTGAAACAGCTTCTGCACCTGATGATCCATTCTCTCTATTCCAAGAAAGAAATCTTCCTGCGTGAACTTATCTCTAACGCCTCCGATGCGGCGGACAAGCTGCGTTTCCGTGCGCTCTCTAACCCGGACCTGTACGAAGGTGATGGCGAACTACGCGTTCGTGTCTCTTTCGATAAAGACAAGCGTACGCTGACCATCTCCGATAACGGCGTGGGGATGACCcgcg                                                                                        <  1:112927/251‑1 (MQ=255)                                                                                                                                                          aaaCTCGTGGTTTTCAGTCAGAAGTGAAACAGCTTCTGCACCTGATGATCCATTCTCTCTATTCCAATAAAGAAATCTTCCTGCGTGAACTTATCTCTAACGCCTCCGATGCGGCGGACAAGCTGCGTTTCCGTGCGCTCTCTAACCCGGACCTGTACGAAGGTGATGGCGAACTACGCGTTCGTGTCTCTTTCGATAAAGACAAGCGTACGCTGACCATCTCCGATAACGGCGTGGGGATGACCCGcgac                                                                                      <  2:101720/251‑1 (MQ=255)                                                                                                                                                               cGTGGTTTTCAGTCAGAAGTGAAACAGCTTCTGCACCTGATGATCCATTCTCTCTATTCCAATAAAGAAATCTTCCTGCGTGAACTTATCTCTAACGCCTCCGATGCGGCGGACAAGCTGCGTTTCCGTGCGCTCTCTAACCCGGACCTGTACGAAGGTGATGGCGAACTACGCGTTCGTGTCTCTTTCGATAAAGACAAGCGTACGCTGACCATCTCCGATAACGGCGTGGGGATGACCCGCGACGaa                                                                                   <  2:397918/249‑1 (MQ=255)                                                                                                                                                                     tttCAGTCAGAAGTGAAACAGCTTCTGCACCTGATGATCCATTCTCTCTATTCCAATAAAGAAATCTTCCTGCGTGAACTTATCTCTAACGCCTCCGATGCGGCGGACAAGCTGCGTTTCCGTGCGCTCTCTAACCCGGACCTGTACGAAGGTGATGGCGAACTACGCGTTCGTGTCTCTTTCGATAAAGACAAGCGTACGCTGACCATCTCCGATAACGGCGTGGGGCTGACCCGCGACGAAGTGATTgc                                                                           >  1:130121/1‑250 (MQ=255)                                                                                                                                                                          gtcagAATTGAAACAGCTGCTGCACCTGATGATCCATTCTCTCTATTCCAATAAAGAAAGCTTCCTGCGTGAACTTATCTCTAACGCCTCCGATGCGGCGGACAAGCTGCGTTTCCGTGCGCTCTCTAACCCGGACCTGTACGAAGGTGATGGCGAACTACGCGTTCGTGTCTCTTTCGATAAAGACAAGCGTACGCTGACCATCTCCGATAACGGCGTGGGGATGACCCGCGACGAAGTGATTGACCATc                                                                      <  2:377747/251‑1 (MQ=255)                                                                                                                                                                            cagAAGTGAAACAGCCTCTGCACCTGATGATCCATTCTCTCTATTCAAATAAAGAAATCTTCCTGCGTGAACTTATCTCTAACGCCTCCGATCAGTCGGCAAAGCTGCGTTTCCGTGCGCTCTCTAACCCGGACCTGTACGAAGGTGATGGCGAACTACGCGTTCGTGTCTCTTTCGATAAAGACCAGCGTACGCTGACCATCTCCGATAACGGCGTGGGGATGGCCCCCGACGAAGTGATTGagcaactt                                                                    >  2:172965/1‑244 (MQ=255)                                                                                                                                                                                aGTGACACAGCTTCTGCACCTGATGATCCATTCTCTCTATTCCACTAAAGACATCTTCCTGCGTGAACTTATCTCTAACGCCTCCGATGCGGCGGACAAGCTGCGTTTCCGTGCGCTCTCTAACCCGGACCTGTACGAAGGTGATGGCGAACTACGCGTTCGTGTCTCTTTCGATAAAGACAAGCGTACGCTGACCATCTCCGATAACGGCGTGGGGCTGACCCGCGACGCAGTGCTTGACCATCTggggg                                                                >  1:344908/1‑250 (MQ=255)                                                                                                                                                                                aGTGAAACAGCTTCTGCACCTGATGATCCATTCTCTCTATTCCAATAAAGAAATCTTCCTGCGTGAACTTATCTCTAACGCCTCCGATGCGGCGGACAAGCTGCGTTTCCGTGCGCTCTCTAACCCGGACCTGTACGAAGGTGATGGCGAACTACGCGTTCGTGTCTCTTTCGATAAAGACAAGCGTACGCTGAc                                                                                                                        <  1:390208/195‑1 (MQ=255)                                                                                                                                                                                aGTGAAACAGCTTCTGCACCTGATGATCCATTCTCTCTATTCCAATAAAGAAATCTTCCTGCGTGAACTTATCTCTAACGCCTCCGATGCGGCGGACAAGCTGCGTTTCCGTGCGCTCTCTAACCCGGACCTGTACGAAGGTGATGGCGAACTACGCGTTCGTGTCTCTTTCGATAAAGACAAGCGTACGCTGAc                                                                                                                        >  2:390208/1‑195 (MQ=255)                                                                                                                                                                                         gCTTCTGCACCTGATGATCCATTCTCTCTATTCCAATAAAGAAATCTTCCTGCGTGAACTTATCTCTAACGCCTCCGATGCGGCGGACAAGCTGCGTTTCCGTGCGCTCTCTAACCCGGACCTGTACGAAGGTGATGGCGAACTACGCGTTCGTGTCTCTTTCGATAAAGACAAGCGTACGCTGACCATCTCCGATAACGGCGTGGGGATGACCCGCGACGAAGTGATTGACCATCTGGGGACTATCGCt                                                        >  1:161216/1‑250 (MQ=255)                                                                                                                                                                                               gCACCTGATGATCCATGCTCTCTATTCCAATAAAGAAATCTTCCTGCGTGAACTTATCTCTAACGCCTCCGATGCGGCGGACAAGCTGCGTTGCCGTGCGCTCTCTAACCCGGACCTGTACGAAGGTGATGGCGAACTACGCGTTCGTGTCTCTTTCGATAAAGACAAGCGTACGCTGACCATCTCCGATAACGGCGTGGGGATGACCCGCGACGAAGTGATTGACCATCTGGGGACTATCGCTAAATcc                                                  <  1:442778/250‑1 (MQ=255)                                                                                                                                                                                                          tCCATTCTCTCTATTCCAATAAAGAAATCTTCCTGCGTGAACTTATCTCTAACGCCTCCGATGCGGCGGACAAGCTGCGTTTCCGTGCGCTCTCTAACCCGGACCTGTACGAAGGTGATGGCGAACTACGCGTTCGTGTCTCTTTCGATAAAGACAAGCGTACGCTGACCATCTCCGATAACGGCGTGGGGATGACCCGCGACGAAGTGATTGACCATCTGGGGACTATCGCTAAATCCGGTACCAAATc                                       >  1:22927/1‑250 (MQ=255)                                                                                                                                                                                                           ccATTCTCTCTATTCCAATAAAGAAATCTTCCTGCGTGAACTTATCTCTAACGCCTCCGATGCGGCGGACAAGCTGCGTTTCCGTGCGCTCTCTAACCCGGACCTGTACGAAGGTGATGGCGAACTACGCGTTCGTGTCTCTTTCGATAAAGACAAGCGTACGCTGACCATCTCCGATAACGGCGTGGGGATGACCCGCGACGAAGTGATTGACCATCTGGGGACTATCGCTAAATCCGGTACCAAATCa                                      <  1:434856/250‑1 (MQ=255)                                                                                                                                                                                                             aTTCTCTCTATTCCAATAAAGAAATCTTCCTGCGTGAACTTATCTCTAACGCCCCCGATGCGGCCGACAAGCTGCGTTTCCGTGCGCTCTCTAACCCGGACCTTTACGAAGGTGATGGCGAACTACGCGTTCGTGTCTCTTTCGATAGAGACAAGCGTACGCTGACCACCTCCGATAACGGCGTGGGGATGACCCGCGACGAAGTGATTGACCATCTGGGGACTATCGCTAAATCCGGTACCAAATCATTc                                   <  1:407048/251‑1 (MQ=255)                                                                                                                                                                                                                   tctATTCCAATAAAGAAATCTTCCTGCGTGAACTTATCTCTAACGCCACCGATGAGGCGGACAAGCTGCGTTTCCGTGCGCTCTCTAACCCGGACCTGTACGAAGGTGATGGCGAACTACGCGTTCGTGTCTCTTTCGATAAAGACAAGCGTACGCTGACCATCTCCGATAACGGCGTGGGGATGACCCGCGCCGACGTGAATGACCATCTGGGGCCTCTCGCCAACTCCGGTACCAAATCAtttctncc                              >  2:415408/1‑244 (MQ=255)                                                                                                                                                                                                                        tCCAATAAAGAAATCTTCCTGCGTGAACTTATCTCTAACGCCTCCGATGCGGCGGACAAGCTGCGTTTCCGTGCGCTCTCTAACCCGGACCTGTACGAAGGTGATGGCGAACTACGCGTTCGTGTCTCTTTCGATAAAGACAAGCGTACGCTGACCATCTCCGATAACGGCGTGGGGATGACCCGCGACGAAGTGATTGACCATCTGGGGACTATCGCTAAATCCGGTACCAAATCATTCCTCGAATccc                         <  1:88282/250‑1 (MQ=255)                                                                                                                                                                                                                              aaaGAAATCTTCATGCGTGAACTTATCTCTAACGCCGCCGATGCGGAGGACAAGCTGCGTCTCCGTGCGCTCTCTAACCCGGACCTGTACGAAGGTGATGGCGAACTACGCGTTCGTGTCTCTTTCGATAAAGACAAGCGTACGCTGACCATCTCCGATAACGGCGTGGGGATGGCCCGCGACGAAGTGATTGACCATCTGGGGACTATCGCTAAATCCGGTACCAAAGCATTCCTCGAATCCCTGGGtt                   <  2:10556/250‑1 (MQ=255)                                                                                                                                                                                                                               aaGAAATCTTCCTGCGTGAACTTATCTCTAACGCCTCCGATGCGGCGGACAAGCTGCGTTTCCGTGCGCTCTCTAACCCGGACCTGTACGAAGGTGATGGCGAACTACGCGTTCGTGTCTCTTTCGATAAAGACAAGCGTACGCTGACCATCTCCGATAACGGCGTGGGGATGACCCGCGACGAAGTGATTGACCATCTGGGGACTATCGCTAAATCCGGTACCAAATCATTCCTCGAATCCCTGGGTTCt                 <  2:50028/251‑1 (MQ=255)                                                                                                                                                                                                                               aaGAAATCTTCCTGCGTGAACTTATCTCTAACGCCTCCGATGCGGCGGACAAGCTGCGTTTCCGTGCGCTCTCTAACCCGGACCTGTACGAAGGTGATGGCGAACTACGCGTTCGTGTCTCTTTCGATAAAGACAAGCGTACGCTGACCATCTCCGATAACGGCGTGGGGATGACCCGCGACGAAGTGATTGACCATCTGGGGACTATCGCTAAATCCGGTACCAAATCATTCCTCGAATCCCTGGGTTCt                 <  1:106073/251‑1 (MQ=255)                                                                                                                                                                                                                                     tCTTCCTGCGTGAACTTATCTCTAACGCCTCCGATGCGGCGGACAAGCTGCGTTTCCGTGCGCTCTCTAACCCGGACCTGTACGAAGGTGATGGCGAACTACGCGTTCGTGTCTCTTTCGATAAAGACAAGCGTACGCTGACCATCTCCGATAACGGCGTGGGGATGACCCGCGACGAAGTGATTGACCATCTGGGGACTATCGCTAAATCCGGTACCAAATCATTCCTCGAATCCCTGGGTTCTGAcc             >  1:327325/1‑249 (MQ=255)                                                                                                                                                                                                                                         ccTGCGTGAACTTATCTCTAACGCCTCCGATGCGGCGGACAAGCTGCGTTTCCGTGCGCTCTCTAACCCGGACCTGTACGAAGGTGATGGCGAACTACGCGTTCGTGTCTCTTTCGATAAAGACAAGCGTACGCTGACCATCTCCGATAACGGCGTGGGGATGACCCGCGACGAAGTGATTGACCATCTGGGGACTATCGCTAAATCCGGTACCAAATCATTCCTCGAATCCCTGGGTTCTGACCAGGCg        <  2:31442/250‑1 (MQ=255)                                                                                                                                                                                                                                           tGCGTGAACTTATCTCTAACGCCTCCGATGCGGCGGACAAGCTGCGTTTCCGTGCGCTCTCTAACCCGGACCTGTACGAAGGTGATGGCGAACTACGCGTTCGTGTCTCTTTCGATAAAGACAAGCGTACGCTGACCATCTCCGATAACGGCGTGGGGATGACCCGCGACGAAAGGATTGACCCTCTGGGGACTATCGCTAAATCCGGTACCAAATCATTCCTCGAATCCCTGGGTTTTGAACAGGCGaa      >  1:412604/1‑250 (MQ=255)                                                                                                                                                                                                                                              gTGAACTTATCTCTAACGCCTCCGATGCGGCGGACAAGCTGCGTTTCCGTGCGCTCTCTAACCCGGACCTGTACGAAGGTGATGGCGAACTACGCGTTCGTGTCTCGTTCGATAAAGACAAGCGTACGCTGACCATCTCCGATAGCGGCGTGGGGATGACCCGCGACGAAGTGATTGACCATCTGGGGACTATCGCTAAATCCGGTACCAAATCATTCCTCGAATCCCTGGGTTCTGACCAGGCGAAAGAc  <  2:342314/251‑1 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                       CCTTGCCGGGCGTCATAAGATTCGTTTTTAAGCAAACGAGAGCAGGATCACCTGCTCTCGCTTGAAATTATTCTCCCTTGTCCCCATCTCTCCCACATCCTGTTTTTAACCTTAAAATGGCATTATTGAGGTAGACCTACATGAAAGGACAAGAAACTCGTGGTTTTCAGTCAGAAGTGAAACAGCTTCTGCACCTGATGATCCATTCTCTCTATTCCAATAAAGAAATCTTCCTGCGTGAGCTTATCTCTAACGCCTCCGATGCGGCGGACAAGCTGCGTTTCCGTGCGCTCTCTAACCCGGACCTGTACGAAGGTGATGGCGAACTACGCGTTCGTGTCTCTTTCGATAAAGACAAGCGTACGCTGACCATCTCCGATAACGGCGTGGGGATGACCCGCGACGAAGTGATTGACCATCTGGGGACTATCGCTAAATCCGGTACCAAATCATTCCTCGAATCCCTGGGTTCTGACCAGGCGAAAGAC  >  NC_000913/494980‑495467

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑