Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,016,983	G→A	A439A (GCC→GCT)	ycbZ ←	putative ATP‑dependent protease YcbZ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,016,983	0	G	A	100.0%	207.9 / NA	62	A439A (GCC→GCT)	ycbZ	putative ATP‑dependent protease YcbZ
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (26/36);  total (26/36)

TTCTACCGCTTTCACCAGTTCACTGTGAAGACTTAAATGGCGAACGTTAGCTGTGGGGATAATGACACCTTGTTTCCCGGTTAACTCACGTTGCTGGCAAATAGCAAAGAAGCCTTCGATTTTCTCATTTAAACCACCGACCGGCTGGGCGCGACCGAACTGATCGACTGAACCT‑GTGATAGCGATACTCTGATTCACCGGCACATCGGCGAGGGCGCTTATCAGGGCGCAGAGTTCAGCCATCGAGGCACTATCTCCATCAACTTCACTGTATGACTGCTCAAATGTCAGCGATGCTGAGAAGGGGATCTGTTGCTCAAGCTGTAGTTCCGACATCAGGAACGCTTGCATGATCATCATCCCTTTCGCATGGATATTGCCGCCAAGCTCCGCTTTGCGTTCGATGTCGGTGAATTCACCATCGCCAATATGCACAACGCAGCTAATGCGAGAAGGTTCGCCAAAAGCGCGTGGATGACCCGGAAATT  >  NC_000913/1016737‑1017224                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                  ttctACCGCTTTCACCAGTTCACTGTGAAGACTTAAATGGCGAACGTTAGCTGTGGGGATAATGACACCTTGTTTCCCGGTTAACTCACGTTGCTGGCAAATAGCAAAGAAGCCTTCGATTTTCTCATTTAAACCACCGACCGGCTGGGCGCGACCGAACTGATCGACTGAACCT‑GTGATAGCGATACTCTGATTCACCGGCACATCGGCGAGGGCGCTTATCAGGGCGCAGAGTTCAGCCATCGAAGCAc                                                                                                                                                                                                                                               >  2:285236/1‑251 (MQ=255)    tACCGCTTTCACCAGTTCACTGTGAAGACTTAAATGGCGAACGTTAGCTGTGGGGATAATGACACCTTGTTTCCCGGTTAACTCACGTTGCTGGCAAATAGCAAAGAAGCCTTCGATTTTCTCATTTAAACCACCGACCGGCTGGGCGCGACCGAACTGATCGACTGAACCT‑GTGATAGCGATACTCTGATTCACCGGCACATCGGCGAGGGCGCTTATCAGGGCGCAGAGTTCAGCCATCGAAGCACTAt                                                                                                                                                                                                                                            >  1:125299/1‑251 (MQ=255)     aCCGCTTTCACCAGTTCACTGTGAAGACTTAAATGGCGAACGTTAGCTGTGGTGATAATGACACCTTGTTTCCCGGTTAACTCACGTTGCTGGCAAATAGCAAAGAAGCCTTCGATTTTCTCATTTAAACCACCGACCGGCTGGGCGCGACCGAACTGATCGACTGAACCT‑GTGATAGCGATACTCTGATTCACCGGCACATCGGCGAGGGCGCTTATCAGGGCGCAGAGTTCAGCCATCGAAGCACTAt                                                                                                                                                                                                                                            <  1:190430/250‑1 (MQ=255)        gCTTTCACCAGTTCTCTGTGAAGACTTAAATGGCGAACGTTAGCTGTGGGGATAATGCCACCTTGTTTCCCGGTTAACTCACGTTGCTGGCAAATAGCAAAGAAGCCTTCGATTTTCTCATTTAAACCACCGACCGGCTGGGCGCGACCGAACTGATCGACTGAACCT‑GTGATAGCGATACTCTGATTCACCGGCACATCGGCGAGGGCGCTTATCAGGGCGCAGAGTTCAGCCATCGAAGCACTATCTcc                                                                                                                                                                                                                                        <  2:249699/251‑1 (MQ=255)              aCCAGTTCACTGTGAAGACTTAAATGGCGAACGTTAGCTGTGGGGATAATGACACCTTGTTTCCCGGTTAACTCACGTTGCTGGCAAATAGCAAAGAAGCCTTCGATTTTCTCATTTAAACCACCGACCGGCTGGGCGCGACCGAACTGATCGACTGAACCT‑GTGATAGCGATACTCTGATTCACCGGCACATCGGCGAGGGCGCTTATCAGGGCGCAGAGTTCAGCCATCGAAGCACTATCTCCATCAAc                                                                                                                                                                                                                                  <  1:293134/251‑1 (MQ=255)              aCCAGTTCACTGTGAAGACTTAAATGGCGAACGTTAGCTGTGGGGATAATGACACCTTGTTTCCCGGTTAACTCACGTTGCTGGCAAATAGCAAAGAAGCCTTCGATTTTCTCATTTAAACCACCGACCGGCTGGGCGCGACCGAACTGATCGACTGAACCT‑GTGATAGCGATACTCTGATTCACCGGCACATCGGCGAGGGCGCTTATCAGGGCGCAGAGTTCAGCCATCGAAGCACTATCTCCATCAAc                                                                                                                                                                                                                                  <  1:201344/251‑1 (MQ=255)                cAGTTCACTGTGAAGACTTAAATGGCGAACGTTAGCTGTGGGGATAATGACACCTTGTTTCCCGGTTAACTCACGTTGCTGGCAAATAGCAAAGAAGCCTTCGATTTTCTCATTTAAACCACCGACCGGCTGGGCGCGACCGAACTGATCGACTGAACCT‑GTGATAGCGATACTCTGATTCACCGGCACATCGGCGAGGGCGCTTATCAGGGCGCAGAGTTCAGCCATCGAAGCACTATCTCCATCAACtt                                                                                                                                                                                                                                <  1:229008/251‑1 (MQ=255)                        tgtgAAGACTTAAATGGCGAACGTTAGCTGTGGGGATAATGACACCTTGTTTCCCGGTTAACTCACGTTGCTGGCAAATAGCAAAGAAGCCTTCGATTTTCTCATTTAAACCACCGACCGGCTGGGCGCGACCGAACTGATCGACTGAACCT‑GTGATAGCGATACTCTGATTCACCGGCACATCGGCGAGGGCGCTTATCAGGGCGCAGAGTTCAGCCATCGAAGCACTATCTCCATCAACTTCACTGtct                                                                                                                                                                                                                        >  1:288651/1‑249 (MQ=255)                              gACTTAAATGGCGAACGTTAGCTGTGGGGATAATGACACCTTGTTTCCCGGTTAACTCACGTTGCTGGCAAATAGCAAAGAAGCCTTCGATTTTCTCATTTAAACCACCGACCGGCTGGGCGCGACCGAACTGATCGACTGAACCT‑GTGATAGCGATACTCTGATTCACCGGCACATCGGCGAGGGCGCTTATCAGGGCGCAGAGTTCAGCCATCGAAGCACTATCTCCATCAACTTCACTGTATGACTGc                                                                                                                                                                                                                  <  1:440665/251‑1 (MQ=255)                                cTTAAATGGCGAACGTTAGCTGTGGGGATAATGACACCTTGTTTCCCGGTTAACTCACGTTGCTGGCAAATAGCAAAGAAGCCTTCGATTTTCTCATTTAAACCACCGACCGGCTGGGCGCGACCGAACTGATCGACTGAACCT‑GTGATAGCGATACTCTGATTCACCGGCACATCGGCGAGGGCGCTTATCAGGGCGCAGAGTTCAGCCATCGAAGCACTATCTCCATCAACTTCACTGTATGACTGCTc                                                                                                                                                                                                                >  2:128632/1‑251 (MQ=255)                                           aaCGTTAGCTGTGGGGATAATGACACCTTGTTTCCCGGTTAACTCACGTTGCTGGCAAATAGCAAAGAAGCCTTCGATTTTCTCATTTAAACCACCGACCGGCTGGGCGCGACCGAACTGATCGACTGAACCT‑GTGATAGCGATACTCTGATTCACCGGCACATCGGCGAGGGCGCTTATCAGGGCGCAGAGTTCAGCCATCGAAGCACTATCTCCATCAACTTCACTGTATGACTGCTCAAATGTCAGCg                                                                                                                                                                                                     >  2:284143/1‑251 (MQ=255)                                           aaCGTTAGCTGTGGGGATAATGACACCTTGTTTCCCGGTTAACTCACGTTGCTGGCAAATAGCAAAGAAGCCTTCGATTTTCTCATTTAAACCACCGACCGGCTGGGCGCGACCGAACTGATCGACTGAACCT‑GTGATAGCGATACTCTGATTCACCGGCACATCGGCGAGGGCGCTTATCAGGGCGCAGAGTTCAGCCATCGAAGCACTATCTCCATCAACTTCACTGTATGACTGCTCAAATGTAAGCg                                                                                                                                                                                                     >  1:370165/1‑251 (MQ=255)                                            aCGTTAGCTGTGGGGATAATGACAACTTGTTTCCCGGATACCTCATGTTGCTGGCAACTAGCCAAGAAGCCTTCGATTTTCTCATTTAAACCACCGACCGGCTGAGCACGACCGCACTGATCGACTGCACCTCATG‑TAGAACTACTCTGATGCACAGGCACAACGGCGACAGCGCTTATCAGCGCGCAGAGTTCAGCCATCGCAGCCCTCTCTCCATAAACTTCACCGTTTGGCTGCTCATATGTTAGctt                                                                                                                                                                                                    >  2:201886/1‑249 (MQ=255)                                                tAGCTGTGGGGATAATGACACCTTGTTTCCCGGTTAACTCACGTTGCTGGCAAATAGCAAAGAAGCCTTCGATTTTCTCATTTAAACCACCGACCGGCTGGGCGCGACCGAACTGATCGACTGAACCT‑GTGATAGCGATACTCTGATTCACCGGCACATCGGCGAGGGCGCTTATCAGGGCGCAGAGTTCAGCCATCGAAGCACTATCTCCATCAACTTCACTGTATGACTGCTCAAATGTCAGCGATGc                                                                                                                                                                                                 <  1:93010/250‑1 (MQ=255)                                                     gtgGGGATAATGACACCTTGTTTCCCGGTTAACTCACGTTGCTGGCAAATAGCAAAGAAGCCTTCGATTTTCTCATTTAAACCACCGACCGGCTGGGCGCGACCGAACTGATCGACTGAACCT‑GTGATAGCGATACTCTGATTCACCGGCACATCGGCGAGGGCGCTTATCAGGGCGCAGAGTTCAGCCATCGAAGCACTATCTCCATCAACTTCACTGTATGACTGCTCAAATGTCAGCGATGCTgag                                                                                                                                                                                             <  1:111474/249‑1 (MQ=255)                                                        gggATAATGACACCTTGTTTCCCGGTTAACTCACGTTGCTGGCAAATAGCAAAGAAGCCTTCGATTTTCTCATTTAAACCACCGACCGGCTGGGCGCGACCGAACTGATCGACTGAACCT‑GTGATAGCGATACTCTGATTCACCGGCACATCGGCGAGGGCGCTTATCAGGGCGCAGAGTTCAGCCATCGAAGCACTATCTCCATAAACTTCAATCTATGACTGCTCCAATGTACGCGATGCTCAGAAggg                                                                                                                                                                                        >  2:12339/1‑251 (MQ=255)                                                           aTAATGACACCTTGTTTCCCGGTTAACTCACGTTGCTGGCAAATAGCAAAGAAGCCTTCGATTTTCTCATTTAAACCACCGACCGGCTGGGCGCGACCGAACTGATCGACTGAACCT‑GTGATAGCGATACTCTGATTCACCGGCACATCGGCGAGGGCGCTTATCAGGGCGCAGAGTTCAGCCATCGAAGCACTATCTCCATCAACTTCACTGTATGACTGCTCAAATGt                                                                                                                                                                                                          <  2:379662/230‑1 (MQ=255)                                                           aTAATGACACCTTGTTTCCCGGTTAACTCACGTTGCTGGCAAATAGCAAAGAAGCCTTCGATTTTCTCATTTAAACCACCGACCGGCTGGGCGCGACCGAACTGATCGACTGAACCT‑GTGATAGCGATACTCTGATTCACCGGCACATCGGCGAGGGCGCTTATCAGGGCGCAGAGTTCAGCCATCGAAGCACTATCTCCATCAACTTCACTGTATGACTGCTCAAATGt                                                                                                                                                                                                          >  1:379662/1‑230 (MQ=255)                                                                  cacCTTGTTTCCCGGTTAACTCACGTTGCTGGCAAATAGCAAAGAAGCCTTCGATTTTCTCATTTAAACCACCGACCGGCTGGGCGCGACCGAACTGATCGACTGAACCT‑GTGATAGCGATACTCTGATTCACCGGCACATCGGCGAGGGCGCTTATCAGGGCGCAGAGTTCAGCCATCGAAGCACTATCTCCATCAACTTCACTGTATGACTGCTCAAATGTCAGCGATGCTGAGAAGGGGATCTGTTGc                                                                                                                                                                              <  2:179523/251‑1 (MQ=255)                                                                   cccTTGTTTCCCGGTTAACTCACGTTGCTGGCAAATAGCAAAGAAGCCTTCGATTTTCTCATTTAAACCACCGACCGGCTGGGCGCGACCGAACTGATCGACTGAACCT‑GTGATAGCGATACTCTGATTCACCGGCACATCGGCGAGGGCGCTTATCAGGGCGCAGAGTTCAGCCATCGAAGCACTATCTCCATCAACTTCACTGTATGACTGCTCAAATGTCAGCGATGCTGAGAAGGGGATCTGTTGCt                                                                                                                                                                             <  1:416190/250‑1 (MQ=255)                                                                    ccTTGTTTCCCGGTTAACTCACGTTGCTTGCAAATAACAAAGAAGCCTACGTTTTTCTCATTTAAACCACCGAACGGCGGGGCGCGACCGAACTGATCGACTGAACCT‑GTGATAGCGATCCTCTGATTTACCCGCACATCGGCGAGGGCGCTTATCAGTGCGCAGTGTTCGGCCAGCGAAGCACTATCTCCATCAACTTCACTGTATGACTGCGCAAATGTCAGCGATGCATAGAAGGCGACCGGTTGCTc                                                                                                                                                                            <  2:247834/251‑1 (MQ=255)                                                                       tGTTTCCCGGTTAACTCACGTTGCTGGCAAATAGCAAAGAAGCCTTCGATTTTCTCATTTAAACCACCGACCGGCTGGGCGCGACCGAACTGATCGACTGAACCT‑GTGATAGCGATACTCTGATTCACCGGCACATCGGCGAGGGCGCTTATCAGGGCGCAGAGTTCAGCCATCGAAGCACTATCTCCATCAACTTCACTGTATGACTGCTCAAATGTCAGCGATGCTGAGAAGGGGATCTGTTGCTCa                                                                                                                                                                           <  2:359406/249‑1 (MQ=255)                                                                               ggTTAACTCACGTTGCTGGCAAATAGCAAAGAAGCCTTCGATTTTCTCATTTAAACCACCGACCGGCTGGGCGCGACCGAACTGATCGACTGAACCT‑GTGATAGAGATACTCTGATTCACCGGCACATCGGCGAGGGCGCTTATCAGGGCGCAGAGTTCAGACATCGAAGCACTATCTCCATCAACTTCACTGTATGACTGCTCAAATGTCCGCGATGCTGAGAAGGGGATCTGTTGCTCAAGCTGTAGtt                                                                                                                                                                 >  2:375725/1‑251 (MQ=255)                                                                                gTTAACTCACGTTGCTGGCAAATAGCAAAGAAGCCTTCGATTTTCTCATTTAAACAACCGACCGGCTGGGCGCGAACGAACTGATCGACTGAACCT‑GTGATAGAGATAATCTGATTCACCGGCACATCGGCGAGGGCGCTTATCAGGGCGTAGAGTTCAGCCAACGAAGCACTATCTCCATCAACTTGACTGTATGACTGCTCAAATGTCAGCGATGCTGAGAAGGGGATCTGTTGCTAAAGCTGTAGTTc                                                                                                                                                                >  1:224705/1‑251 (MQ=255)                                                                                      tCACGTTGCTGGCAAATAGCAAAGAAGCCTTCGATTTTCTCATTTAAACCACCGACCGGCTGGGCGCGACCGAACTGATCGACTGAACCT‑GTGATAGCGATACTCTGATTCACCGGCACATCGGCGAGGGCGCTTATCAGGGCGCAGAGTTCAGCCATCGAAGCACTATCTCCATCAACTTCACTGTATGACTGCTCAAATGTCAGCGATGCTGAGAAGGGGATCTGTTGCTCAAGCTGTAGTTCCGACAt                                                                                                                                                          >  2:82815/1‑251 (MQ=255)                                                                                        aCGTTGCTGGCAAATAGCAAAGAAGCCTTCGATTTTCTCATTTAAACCACCGACCGGCCGGGCGCGACCGAACTGATCGACTGAACCT‑GTGATAGCGATACTCTGATTCACCGGCACATCGGCGAGGGCGCTTATCAGGGCGCAGAGTTCAGCCATCGAAGCACTATCTCCATCAACTTCACTGTATGACTGCTCAAATGTCAGCGATGCTGAGAAGGGGATCTGTTGCTCAAGCTGTAGTTCCGACATCa                                                                                                                                                        <  2:105793/251‑1 (MQ=255)                                                                                                     aTAGCAAAGAAGCCTTCGATTTTCTCATTTAAACCACCGACCGGCTGGGCGCGACCGAACTGATCGACTGAACCT‑GTGATAGCGATACTCTGATTCACCGGCACATCGGCGAGGGCGCTTATCAGGGCGCAGAGTTCAGCCATCGAAGCACTATCTCCATCAACTTCACTGTATGACTGCTCAAATGTCAGCGATGCTGAGAAGGGGATCTGTTGCTCAAGCTGTAGTTCCGACATCAGGAACGCTTGCAt                                                                                                                                           >  1:141380/1‑251 (MQ=255)                                                                                                            agaagtatgtgATTTTCTCATTTAAACCGCCGACCGGCTCGGCGCGACCGCTCTTATCGACTGAACCT‑GTGATAGCGAGACTCTGATTCACCGTCTCATCTGCGAGGGCGCTTATCAGTGCGCAGAGTTCATCCTTCGTAGCACTATCTCCATCAACTTCACTGTATGACTTCTCAAATGTCAGCGATGCTGAGAAGGTTATCTGTTGCTCAAGCTGTAGTTCCGACATTAGGAACGCTTGCATGatcatt                                                                                                                                    <  2:391818/241‑2 (MQ=255)                                                                                                             gaagCCTTCGATTTTCTCATTTAAACCACCGACCGGCTGGGCGCGACCGAACTGATCGACTGAACCT‑GTGATAGCGATACTCTGATTCACCGGCACATCGGCGAGGGCGCTTATCAGGGCGCAGAGTTCAGCCATCGAAGCACTATCTCCATCAACTTCACTGTATGACTGCTCAAATGTCAGCGATGCTGAGAAGGGGATCTGTTGCTCAAGCTGTAGTTCCGACATCAGGAACGCTTGCATGatcatca                                                                                                                                   <  1:82815/251‑1 (MQ=255)                                                                                                                    tCGATTTTCTCATTTAAACCACCGACCGGCTGGGCGCGACCGAACTGATCGACTGAACCT‑GTGATAGCGATACTCTGATTCACCGGCACATCGGCGAGGGCGCTTATCAGGGCGCAGAGTTCAGCCATCGAAGCACTATCTCCATCAACTTCACTGTATGACTGCTCAAATGTCAGCGATGCTGAGAAGGGGATCTGTTGCTCAAGCTGTAGTTCCGACATCAGGAACGCTTGCATGATCATCATCCCt                                                                                                                              <  2:383421/249‑1 (MQ=255)                                                                                                                    tCGATTTTCTCATTTAAACCACCAACCGGCTGGGCGCGACCGAACTGATCGACTGAACCT‑GTGATAGCGATACTCTGATTCACCGGCACATCGGCGAGGGCGCTTATCAGGGCGCAGAGTTCAGCCATCGAAGCACTATCTCCATCAACTTCACTGTATGACTGCTCAAATGTCAGCGATGCTGAGAAGGGGATCTGTTGCTCAAGCTGTAGTTCCGACATCAGGAACGCTTGCATGATCATCATCCCt                                                                                                                              <  2:338922/249‑1 (MQ=255)                                                                                                                     cGATTTTCTCATTTAAACCACCGACCGGCTGGGCGCGACCGAACTGATCGACTGAACCT‑GTGATAGCGATACTCTGATTCACCGGCACAGCGGCGAGGGCGCTTATCAGGGCGCAGAGTTCAGCCATCGAAGCACTATCTCCATCAACTTCACTGTATGACTGCTCAAATGTCAGCGATGCTGAGAAGGGGATCTGTTGCTCAAGCTGTAGTTCCGACATCAGGAACGCTTGCATGATCATCATCCCTTTc                                                                                                                           <  1:392266/251‑1 (MQ=255)                                                                                                                      gATTTTCTCATTTAAACCACCGACCGGCTGGGCGCGACCGAACTGATCGACTGAACCT‑GTGATAGCGATACTCTGATTCACCGGCACATCGGCGAGGGCGCTTATCAGGGCGCAGAGTTCAGCCATCGAAGCACTATCTCCATCAACTTCACTGTATGACTGCTCAAATGTCAGCGATGCTGAGAAGGGGATCTGTTGCTCAAGCTGTAGTTCCGACATCAGGAACGCTTGCATGATCATCATCCCTTTCg                                                                                                                          <  1:221016/251‑1 (MQ=255)                                                                                                                       aTTTTCTCATTTAAACCACCGACCGGCTGGGCGCGACCGAACTGATCGACTGAACCT‑GTGATAGCGATACTCTGATTCACCGGCACATCGGCGAGGGCGCTTATCAGGGCGCAGAGTTCAGCCATCGAAGCACTATCTCCATCAACTTCACTGTATGACTGCTCAAATGTCAGCGATGCTGAGAAGGGGATCTGTTGCTCAAGCTGTAGTTCCGACATCAGGAACGCTTGCATGATCATCATCCCTTTCGc                                                                                                                         <  1:128632/251‑1 (MQ=255)                                                                                                                          ttCTCATTTAAACCACCGACCGGCTGGGCGCGACCGAACTGATCGACTGAACCT‑GTGATAGCGATACTCTGATTCACCGGCACATCGGCGAGGGCGCTTATCAGGGCGCAGAGTTCAGCCATCGAAGCACTATCTCCATCAACTTCACTGTATGACTGCTCAAATGTCAGCGATGCTGAGAAGGGGATCTGTTGCTCAAGCTGTAGTTCCGACATCAGGAACGCTTGCATGATCATCATCCCTTTCGCATg                                                                                                                      >  2:229232/1‑251 (MQ=255)                                                                                                                                     accaccGAGCGGCTGGGCGCGACCGAACTGATCGACTGAACCT‑GTGATAGCGATACTCTGATTCACCGGCACATCGGCGAGGGCGCTTATCAGGGCGCAGAGTTCAGCCATCGAAGCACTATCTCCATCAACTTCACTGTATGACTGCTCAAATGTCAGCGATGCTGAGAAGGGGATCTGTTGCTCAAGCTGTAGTTCCGACATCAGGAACGCTTGCATGATCATCATCCCTTTCGCATGGATATTgccgc                                                                                                           <  2:78320/251‑1 (MQ=255)                                                                                                                                      ccaccGACCGGCTGGGCGCGACCGAACTGATCGACTGAACCT‑GTGATAGCGATACTCTGATTCACCGGCACATCGGCGAGGGCGCTTATCAGGGCGCAGAGTTCAGCCATCGAAGCACTATCTCCATCAACTTCACTGTATGACTGCTCAAATGTCAGCGATGCTGAGAAGGGGATCTGTTGCTCAAGCTGTAGTTCCGACATCAGGAACGCTTGCATGATCATCCTCCCTTTCGCATGGATATTgccgcc                                                                                                          <  1:114895/251‑1 (MQ=255)                                                                                                                                       caccGACCGGCTGGGCGCGACCGAACTGATCGACTGAACCT‑GTGATAGCGATACTCTGATTCACCGGCACATCGGCGAGGGCGCTTATCAGGGCGCAGAGTTCAGCCATCGAAGCACTATCTCCATCAACTTCACTGTATGACTGCTCAAATGTCAGCGATGCTgaga                                                                                                                                                                                            >  2:193227/1‑168 (MQ=255)                                                                                                                                       caccGACCGGCTGGGCGCGACCGAACTGATCGACTGAACCT‑GTGATAGCGATACTCTGATTCACCGGCACATCGGCGAGGGCGCTTATCAGGGCGCAGAGTTCAGCCATCGAAGCACTATCTCCATCAACTTCACTGTATGACTGCTCAAATGTCAGCGATGCTgaga                                                                                                                                                                                            <  1:193227/168‑1 (MQ=255)                                                                                                                                       caccGACCGGCTGGGCGCGACCGAACTGATCGACTGAACCT‑GTGATAGCGATACTCTGATTCACCGGCACATCGGCGAGGGCGCTTATCAGGGCGCAGAGTTCAGCCATCGAAGCACTATCTCCATCAACTTCACTGTATGACTGCTCAAATGTCAGCGATGCTGAGAAGGGGATCTGTTGCTCAAGCTGTAGTTCCGACATCAGGAACGCTTGCATGATCATCCTCCCTTTCGCATGGATATTgcctcc                                                                                                          >  2:235119/1‑247 (MQ=255)                                                                                                                                         ccgaccgGCTGGGCGCGACCGAACTGATCGACTGAACCT‑GTGATAGCGATACTCTGATTCACCGGCACATCGGCGAGGGCGCTTATCAGGGCGCAGAGTTCAGCCATCGAAGCACTATCTCCATCAACTTCACTGTATGACTGCTCAAATGTCAGCGATGCTGAGAAGGGGATCTGTTGCTCAAGCTGTAGTTCCGACATCAGGAACGCTTGCATGATCATCATCCCTTTAGCATGGATATTGCCGCCa                                                                                                         >  2:423739/1‑249 (MQ=255)                                                                                                                                           gaccgGCTGGGCGCGACCGAACTGATCGACTGAACCT‑GTGATAGCGATACTCTGATTCACCGGCACATCGGCGAGGGCGCTTATCAGGGCGCAGAGTTCAGCCATCGAAGCACTATCTCCATCAACTTCACTGTATGACTGCTCAAATGTCAGCGATGCTGAGAAGGGGATCTGTTGCTCAAGCTGTAGTTCCGACATCAGGAACGCTTGCATGATCATCATCCCTTTCGCATGGATATTGCCGCCAAGCt                                                                                                     <  1:275748/251‑1 (MQ=255)                                                                                                                                                     gcgcGACCGAACTGATCGACTGAACCT‑GTGATAGCGATACTCTGATTCACCGGCACATCGGCGAGGGCGCTTATCAGGGCGCAGAGTTCAGCCATCGAAGCACTATCTCCATCAACTTCACTGTATGACTGCTCAAATGTCAGCGATGCTGAGAAGGGGATCTGTTGCTCAAGCTGTAGTTCCGACATCAGGAACGCTTGCATGATCATCATCCCTTTCGCATGGATATTGCCGCCAAGCTCCGCTTTGCg                                                                                           <  2:141380/251‑1 (MQ=255)                                                                                                                                                        cgACCGAACTGATCGACTGAACCT‑GTGATAGCGATACTCTGATTCACCGGCACATCGGCGAGGGCGCTTATCAGGGCGCAGAGTTCAGCCATCGAAGCACTATCTCCATCAACTTCACTGTATGACTGCTCAAATGTCAGCGATGCTGAGAAGGGGATCTGTTGCTCAAGCTGTAGTTCCGACATCAGGAACGCTTGCAGGATCATCATCCCTTTCGCATGGATATTGCCGCCAAGCTCCGCTTTGCGTTc                                                                                        <  2:176278/251‑1 (MQ=255)                                                                                                                                                         gACCGAACTGATCGACTGAACCT‑GTGATAGCGATACTCTGATTCACCGGCACATCGGCGAGGGCGCTTATCAGGGCGCAGAGTTCAGCCATCGAAGCACTATCTCCATCAACTTCACTGTATGACTGCTCAAATGTCAGCGATGCTGAGAAGGGGATCTGTTGCTCAAGCTGTAGTTCCGACATCAGGAACGCTTGCATGATCATCATCCCTTTCGCATGGATATTGCCGCCAAGCTCCGCTTTGCGTTCg                                                                                       >  1:100629/1‑251 (MQ=255)                                                                                                                                                              aaCTGATCGACTGAACCT‑GTGATAGCGATACTCTGATTCACCGGCACATCGGCGAAGGCGCTTATCAGGGCGCAGAGTTCAGCCATCGAAGCACTATCTCCATCAACTTCACTGTATGACTGCTCAAATGTCAGCGATGCTGAGAAGGGGACCTGTTGATCAAGCTGTAGTTCCGACATCAGGAACGCTTGCATGATCATCATCCCTTTCGCATGGATATTGCCGCCAAGCTCCGCTTTGCGTTCgctgac                                                                                  >  2:71736/1‑246 (MQ=255)                                                                                                                                                                     cGACTGAACCT‑GTGATAGCGATACTCTGATTCACCGGCACATCGGCGAGGGCGCTTATCAGGGCGCAGAGTTCAGCCATCGAAGCACTATCTCCATCAACTTCACTGTATGACTGCTCAAATGTCAGCGATGCTGAGAAGGGGATCTGTTGCTCAAGCTGTAGTTCCGACATCAGGAACGCTTGCATGATCATCATCCCTTTCGCATGGATATTGCCGCCAAGCTCCGCTTTGCGTTCGATGTCGGTGaa                                                                            <  1:284143/250‑1 (MQ=255)                                                                                                                                                                      gACTGAACCT‑GTGATAGCGATACTCTGATTCACCGGCACATCGGCGAGGGCGCTTATCAGGGCGCAGAGTTCAGCCATCGAAGCACTATCTCCATCAACTTCACTGTATGACTGCTCAAATGTCAGCGATGCTGAGAAGGGGATCTGTTGCTCAAGCTGTAGTTCCGACATCAGGAACGCTTGCATGATCATCATCCCTTTCGCATGGATATTGCCGCCAAGCTCCGCTTTGCGTTCGATGTCGGTGAAtt                                                                          >  2:443080/1‑251 (MQ=255)                                                                                                                                                                             ccT‑GTGATAGCGATACTCTGATTCACCGGCACATCGGCGAGGGCGCTTATCAGGGCGCAGAGTTCAGCCATCGAAGCACTATCTCCATCAACTTCACTGTATGACTGCTCAAATGTCAGCGATGCTGAGAAGGGGATCTGTTGCTCAAGCTGTAGTTCCGACATCAGGAACGCTTGCATGATCATCATCCCTTTCGCATGGATATTGCCGCCAAGCTCCGCTTTGCGTTCGATGTCGGTGAATTCACCATc                                                                   >  2:139166/1‑251 (MQ=255)                                                                                                                                                                              cT‑GTGATAGCGATACTCTGATTCACCGGCACATCGGCGAGGGCGCTTATCAGGGCGCAGAGTTCAGCCATCGAAGCACTATCTCCATCAACTTCACTGTATGACTGCTAAAATGTCAGCGATGCTGAGAAGGGGATCTGTTGCTCAAGCTGTAGTTCCGACATCAGGAACGCTTGCATGATCATCATCCCTTTCGCATGGATATTGCCGCCAAGCTCCGCTTTGCGTTCGATGTCGGTGAATTCACCATCg                                                                  >  1:83656/1‑251 (MQ=255)                                                                                                                                                                                      aGCGATACTCTGATTCACCGGCACATCGGCGAGGGCGCGGATCAGGGCGCAGAGTTCAGCCATCGCAGCACTATCTCCATCAACTTCACTGTATGACTTCTCAAATGTCAGCGATGCTGAGAAGGGGATCTGTTGCTCAAGCTGTAGTTCCGACATCATGAACGCTTGCATGATCATCATCCCTTTCTCATGGATATTGCCGCCAAGCTCCGCTTTGCGTTCGATGTCGGTGAAGTCACCATCTCCAata                                                            <  1:344129/250‑1 (MQ=255)                                                                                                                                                                                          aaaaactGATTCACCGGCACATCGGCGAGGGCGATTATCAGGGCGCAGAGTTCAGACATAGAAGCACTATCGCGATCAAATTCACTGTATGAATGCTCAAATGTCAGCGATGCTGAGAAGGGGATCTGTTGATCAAGCTGTAGTTCCGACATCAGGAACGCTTGCATGATCATCATCACTTACGCATGGATAGTGCCGACAAGATCCGCTTTGCGTTAGATGTCGGTGAATTCACCATCGCCAATATGcac                                                       <  2:129650/246‑1 (MQ=255)                                                                                                                                                                                            aCTCTGCTTCACCGGCACATCGGCGAGGGCGCTTATCAGGGCGCAGAGTTCAGCCATCGAAGCACTATCTCCATCAACTTCACTGTATGACTGCTCAAATGTCAGCGATGCTGAGAAGGGGATCTGTTGCTCAAGCTGTAGTTCCGACATCAGGAACGCTTGCATGATCATCATCCCttt                                                                                                                            >  1:361529/1‑180 (MQ=255)                                                                                                                                                                                            aCTCTGATTCACCGGCACATCGGCGAGGGCGCTTATCAGGGCGCAGAGTTCAGCCATCGAAGCACTATCTCCATCAACTTCACTGTATGACTGCTCAAATGTCAGCGATGCTGAGAAGGGGATCTGTTGCTCAAGCTGTAGTTCCGACATCAGGAACGCTTGCATGATCATCATCCCttt                                                                                                                            <  2:361529/180‑1 (MQ=255)                                                                                                                                                                                            aCTCTGATTCACCGGCACATCGGCGAGGGCGCTTATCAGGGCGCAGAGTTCAGCCATCGAAGCACTATCTCCATCAACTTCACTGTATGACTGCTCAAATGTCAGCGATGCTGAGAAGGGGATCTGTTGCTCAAGCTGTAGTTCCGACATCAGGAACGCTTGCATGATCATCATCCCTTTCGCATGGATATTGCCGCCAAGCTCCGCTTTGCGTTCGATGTCGGTGAATTCACCATCGCCAATATGcaca                                                      >  1:246522/1‑250 (MQ=255)                                                                                                                                                                                                 gATTCACCGGCACATCGGCGAGGGCGCTTATCAGGGCGCAGAGTTCAGCCATCGAAGCACTATCTCCATCAACTTCACTGTATGACTGCTCAAATGTCAGCGATGCTGAGAAGGGGATCTGTTGCTCAAGCTGTAGTTCCGACATCAGGAACGCTTGCATGATCATCATCCCTTTCGCATGGATATTGCCGCCAAGCTCCGCTTTGCGTTCGATGTCGGTGAATTCACCATCGCCAATATGCACAACGc                                                  <  2:77255/249‑1 (MQ=255)                                                                                                                                                                                                    tCACCGGCACATCGGCGAGGGCGCTTATCAGGGCGCAGAGTTCAGCCATCGAAGCACTATCTCCATCAACTTCACTGTATGACTGCTCAAATGTCAGCGATGCTGAGAAGGGGATCTGTTGCTCAAGCTGTAGTTCCGACATCAGGAACGCTTGCATGATCATCATCCCTTTCGCATGGATATTGCCGCCAAGCTCCGCTTTGCGTTCGATGTCGGTGAATTCACCATCGCCAATATGCACAACGCAGCt                                              <  1:269589/250‑1 (MQ=255)                                                                                                                                                                                                                 ggCGAGGGCGCTTATCAGGGCGCAGAGTTCAGCCATCGAAGCACTATCTCCATCAACTTCACTGTATGACTGCTCAAATGTCAGCGATGCTGAGAAGGGGATCTGTTGCTCAAGCTGTAGTTCCGACATCAGGAACGCTTGCATGATCATCATCCCTTTCGCATGGATATTGCCGCCAAGCTCCGCTTTGCGTTCGATGTCGGTGAATTCACCATCGCCAATATGCACAACGCAGCTAATGCGAGAAGGtt                                <  1:139166/251‑1 (MQ=255)                                                                                                                                                                                                                    gAGGGCGCTTATCAGGGCGCAGAGTTCAGCCATCGAAGCACTATCTCCATCAACTTCACTGTATGACTGCTCAAATGTCAGCGATGCTGAGAAGGGGATCTGTTGATCAAGCTGTAGTTCCGACATCAGGAACGCTTGCATGATCATCATCCCTTTCGCATGGATATTGCCGCCAAGCTCCGCTTTGCGTTCGATGTCGGTGAATTCACCATCGCCAATATGCACAACGCAGCTAATGCGAGAAGGTTCGc                             >  2:288037/1‑251 (MQ=255)                                                                                                                                                                                                                      gggCGCTTATCAGGGCGCAGAGTTCAGCCATCGAAGCACTATCTCCATCAACTTCACTGTATGACTGCTCAAATGTCAGCGATGCTGAGAAGGGGATCTGTTGCTCAAGCTGTAGTTCCGACATCAGGAACGCTTGCATGATCATCATCCCTTTCGCATGGATATTGCCGCCAAGCTCCGCTTTGCGTTCGATGTCGGTGAATTCACCATCGCCAATATGCACAACGCAGCTAATGCGAGAAGGTTCGCCa                           <  1:375725/251‑1 (MQ=255)                                                                                                                                                                                                                                         gagTTCAGCCATCGAAGCACTATCTCCATCAACTTCACTGTATGACTGCTCAAATGTCAGCGATGCTGAGAAGGGGATCTGTTGCTCAAGCTGTAGTTCCGACATCAGGAACGCTTGCATGATCATCATCCCTTTCGCATGGATATTGCCGCCAAGCTCCGCTTTGCGTTCGATGTCGGTGAATTCACCATCGCCAATATGCACAACGCAGCTAATGCGAGAAGGTTCGCCAAAAGCGCGTGGATGACCCg        >  1:330402/1‑251 (MQ=255)                                                                                                                                                                                                                                               aGCCATCGAAGCACTATCTCCATCAACGTCACTGTATGACTGCTCAAATGTCAGCGATGCTGAGAAGGGGATCTGTTGCTCAAGCTGTAGTTCCGACATCAGGAACGCTTGCATGATCATCATCCCTTTCGCATGGATATTGCCGCCAAGCTCCGCTTTGCGTTCGATGTCGGTGAATTCACCATCGCCAATATGCACAACGCAGCTAATGCGAGAAGGTTCGCCAAAATCGCGTGGATGACCCGGAAAtt  <  2:78275/251‑1 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                  TTCTACCGCTTTCACCAGTTCACTGTGAAGACTTAAATGGCGAACGTTAGCTGTGGGGATAATGACACCTTGTTTCCCGGTTAACTCACGTTGCTGGCAAATAGCAAAGAAGCCTTCGATTTTCTCATTTAAACCACCGACCGGCTGGGCGCGACCGAACTGATCGACTGAACCT‑GTGATAGCGATACTCTGATTCACCGGCACATCGGCGAGGGCGCTTATCAGGGCGCAGAGTTCAGCCATCGAGGCACTATCTCCATCAACTTCACTGTATGACTGCTCAAATGTCAGCGATGCTGAGAAGGGGATCTGTTGCTCAAGCTGTAGTTCCGACATCAGGAACGCTTGCATGATCATCATCCCTTTCGCATGGATATTGCCGCCAAGCTCCGCTTTGCGTTCGATGTCGGTGAATTCACCATCGCCAATATGCACAACGCAGCTAATGCGAGAAGGTTCGCCAAAAGCGCGTGGATGACCCGGAAATT  >  NC_000913/1016737‑1017224

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑