Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,538,412	C→T	G148S (GGC→AGC)	narY ←	nitrate reductase Z subunit beta

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,538,412	0	C	T	100.0%	147.1 / NA	45	G148S (GGC→AGC)	narY	nitrate reductase Z subunit beta
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (24/21);  total (24/21)

TTTTGTACGGACAACCGCTTATGCACAAACGCCAGCCACGGCATTTATCCTGATCAATCAGCACAATGCCGTCTTCTTCGCGTTTGTAGATAGCGCCGCTTGGGCAGGTCGCCACGCAGCTGGGATTGAGGCAGTGTTCGCACAGGCGCGGCAGGTACATCATGAAGGTGTTTTCAAACTGCCCGTACATCTCCTTTTGCATGGCCTCGAAGTTGCGGTCGCGGGCACGTTTTTCGAACTCACCGCCCAGCAGTTCTTCCCAGTTTGGCCCCCAGATCACTTTGTCCATCCGTTTGCCGTCAATCAGTGAACGCGGGCGAGCAGTAGGAATATGTTTGCCTTCCGGTGCGCTATGCAAATGTTCGTAGTCGAAGGTGAAAGGTTCGTAGTAATCATCAATCTGCGGCACCACCGGGTTGGCGAAGATTTTGGTTATTACGCCCATCTTGTTGCCCAGACGCGGGCGTATCTTGCCATTCACATCACGCACCCA  >  NC_000913/1538166‑1538658                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                       ttttGTACGGACAACCGCTTATGCACAAACGCCAGCCACGGCATTTATCCTGATCAATCAGCACAATGCCGTCTTCTTCGCGTTTGTAGATAGCGCCGCTTGGGCAGGTCGCCACGCAGCTGGGATTGAGGCAGTGTTCGCACAGGCGCGGCAGGTACATCATGAAGGTGTTTTCAAACTGCCCGTACATCTCCTTTTGCATGGCCTCGAAGTTGCGGTCGTGGGCACGTTTTTCGAACTCACCGCTcagc                                                                                                                                                                                                                                                    >  2:219541/1‑251 (MQ=255)                 gCTTATGCACAAACGCCAGCCACGGCATTTATCCTGATCAATCAGCACAATGCCGTCTTCTTCGCGTTTGTAGATAGCGCCGCTTGGGCAGGTCGCCACGCAGCTGGGATTGAGGCAGTGTTCGCACAGGCGCGGCAGGTACATCATGAAGGTGTTTTCAAACTGCCCGTACATCTCCTTTTGCATGGCCTCGAAGTTGCGGTCGTGGGCACGTTTTTCGAACTCACCGCTCAGCAGTTCTTCCCAGTTTg                                                                                                                                                                                                                                    >  1:42155/1‑251 (MQ=255)                   ttATGCACAAACGCCAGCCACGGCATTTATCCTGATCAATCAGCACAATGCCGTCTTCTTCGCGTTTGTAGATAGCGCCGCTTGGGCAGGTCGCCACGCAGCTGGGATTGAGGCAGTGTTCGCACAGGCGCGGCAGGTACATCATGAAGGTGTTTTCAAACTGCCCGTACATCTCCTTTTGCATGGCCTCGAAGTTGCGGTCGTGGGCACGTTTTTCGAACTCACCGCTCCGCAGTTCTTCCCAGTTTGGc                                                                                                                                                                                                                                  >  2:114490/1‑251 (MQ=255)                      tGCACAAACGCCAGCCACGGCCTTTATCCTGATCAATCAGCACAATGCCGTCTTCTTCGCGTTTGTAGATAGCGCCGCTTGGGCAGGTCGCCACGCAGCTGGGATTGAGGCAGTGTTCGCACAGGCGCGGCAGGCACATCATTAAGGTGTTTTCAAACTGCCCGTACATCTCCTTTTGCATGGCCTCGAAGTTGCGGTCGCGGGCACGTTTTTCTAACTCACCGCTCCTCAGTTCTTCCCCGTTTGGcccc                                                                                                                                                                                                                               >  2:196749/1‑251 (MQ=255)                               gccagccaCGGCATTTATCCTGATCAATCAGCACAATGCCGTCTGCTTCGCGTTTGTAGATAGCGCCGCTTGGGCAGGTCGCCACGCAGCTGGGATTGAGGCAGTGTTCGCACAGGCGCGGCAGGTACATCATGAAGGTGTTTTCAAACTGCCCGTACATCTCCTTTTGCATGGCCTCGAAGTTGCGGTCGTGGGCACGTTTTTCGAACTCACCGCTCAGCAGTTCTTCCCAGTTTGGCCCCCAGATCACt                                                                                                                                                                                                                      <  1:202611/251‑1 (MQ=255)                                    ccaNGGCATTTATCCTGATCAATCAGCACAATGCCGTCTTCTTCGCGTTTGTAGATAGCGCCGCTTGGGCAGGTCGCCACGCAGCTGGGATTGAGGCAGTGTTCGCACAGGCGCGGCAGGTACATCATGAAGGTGTTTTCAAACTGCCCGTACATCTCCTTTTGCATGGCCTCGAAGTTGCGGTCGTGGGCACGTTTTTCGAACTCACCGCTCAGCAGTTCTTCCCAGTTTGGCCCCCAGATCACTTTGTc                                                                                                                                                                                                                 <  2:406780/251‑1 (MQ=255)                                        ggCATTTATCCTGATCAATCAGCACAATGCCGTCTTCTTCGCGTTTGTAGATAGCGCCGCTTGGGCAGGTCGCAACGCAGCTGGGATTGAGGCAGTGTTCGCACAGGCGCGGCAGGTACATCATGAAGGTGTTTTCAAACTGCCCGTACATCTCCTTTTGAATGGCCTCGAAGTTGCGGTCGTGGGCACGTTTTTCGAACTCATCGCTCAGCAGTTCTTCTCAGTTTGGCCCCCAGATCAccttttccctc                                                                                                                                                                                                             >  1:147256/1‑241 (MQ=255)                                                            aGCACAATGCCGTCTTCTTCGCGTTTGTAGATAGCGCCGCTTGGGCAGGTCGCCACGCAGCTGGGATTGAGGCAGTGTTCGCACAGGCGCGGCAGGTACATCATGAAGGTGTTTTCAAACTGCCCGTACATCTCCTTTTGCATGGCCTCGAAGTTGCGGTCGTGGGCACGTTTTTCGAACTCACCGCTCAGCAGTTCTTCCCAGTTTGGCCCCCAGATCACTTTGTCCATCCGTTTGCCGTCAATCAGTg                                                                                                                                                                                          >  1:125014/1‑250 (MQ=255)                                                                caATGCCGTCTTCTTCGCGTTTGTAGATAGCGCCGCTTGGGCAGGTCGCCACGCAGCTGGGATTGAGGCAGTGTTCGCACAGGCGCGGCAGGTACATCATGAAGGCGATTTCAAACTGCCCGTACATCTCCTTTTGCATGGCCTCGAAGTTGCGGTCGTGGGCACGTTTTTCGAACTCACCGCTCAGCAGTTCTTCCCAGTTTGGCCCCCAGATCACTGTGTCCATCCGTTTGCCGTCAATCAGTGAAcgc                                                                                                                                                                                     >  1:244033/1‑251 (MQ=255)                                                                    gCCGTCTTCTTCGCGTTTGTAGATAGCGCCGCTTGGGCAGGTCGCCACGCAGCTGGGATTGAGGCAGTGTTCGCACAGGCGCGGCAGGTACATCATGAAGGTGTTTTCAAACTGCCCGTACATCTCCTTTTGCATGGCCTCGAAGTTGCGGTCGTGGGCACGTTTTTTGAACTCACCGCTCAGCAGTTCTTCCCAGTTTGGCCCCCAGATCACTTTGTCCCTCCGTTTGCCGGCAATCCGTGAACGCGGGc                                                                                                                                                                                 >  2:224615/1‑251 (MQ=255)                                                                    gCCGTCTTCTTCGCGTTTGTAGATAGCGCCGCTTGGGCAGGTCGCCACGCAGCTGGGATTGAGGCAGTGTTCGCACAGGCGCGGCAGGTACATCATGAAGGTGTTTTCAAACTGCCCGTACATCTCCTTTTGCATGGCCTCGAAGTTGCGGTCGTGGGCACGTTTTTCGAACTCACCGCTCAGCAGTTCTTCCCAGTTTGGCCCCCAGATCACTTTGTCCATCCGTTTGCCGTCAATCAGTGAACGCggg                                                                                                                                                                                  <  1:114490/250‑1 (MQ=255)                                                                           tcttcGCGTTTGTAGATAGCGCCGCTTGGGCAGGTCGCCACGCAGCTGGGATTGAGGCAGTGTTCGCACAGGCGCGGCAGGTACATCATGAAGGTGTTTTCAAACTGCCCGTACATCTCCTTTTGCATGGCCTCGAAGTTGCGGTCGTGGGCACGTTTTTCGAACTCACCGCTCAGCAGTTCTTCCCAGTTTGGCCCCCAGATCACTTTGTCCATCCGTTTTCCGTCAATCAGTGAACGCGGGCGAGc                                                                                                                                                                             <  1:66123/248‑1 (MQ=255)                                                                                    ttGTAGATAGCGCCGCTTGGGCAGGTCGCCACGCAGCTGGGATTGAGGCAGTGTTCGCACAGGCGCGGCAGGTACATCATGAAGGTGTTTTCAAACTGCCCGTACATCTCCTTTTGCATGGCCTCGAAGTTGCGGTCGTGGGCACGTTTTTCGAACTCACCGCTCAGCAGTTCTTCCCAGTTTGGCCCCCAGATCACTTTGTCCATCCGTTTGCCGTCAATCAGTGAACGCGGGCGAGCAGTAGGAatat                                                                                                                                                                  <  1:18941/250‑1 (MQ=255)                                                                                     tGTAGATAGCGCTGCTTGGGCAGGTCGCCACGCAGCTGGGATTGAGGCAGTGTTCGCACAGGCGCGGCAGGTACATCATGAAGGTGTTTTCAAACTGCCCGTACATCTCCTTTTGCATGGCCTCGAAGTTGCGGTCGTGGGCACGTTTTTCGAACTCACCGCTCAGCAGTTCTTCCCAGTTTGGCCCCCAGATCACTTTGTCCATCCGTTTGCCGTCAATCAGTGAACGCGGGCGAGCAGTAGGAATATg                                                                                                                                                                 <  1:419256/250‑1 (MQ=255)                                                                                        aGATAGCGCCGCTTGGGCAGGTCGCCACGCAGCTGGGATTGAGGCAGTGTTCGCACAGGCGCGGCAGGTACATCATGAAGGTGTTTTCAAACTGCCCGTACATCTCCTTTTGCATGGCCTCGAAGTTGCGGTCGTGGGCACGTTTTTCGAACTCACCGCTCAGCAGTTCTTCCCAGTTTGGCCCCCAGATCACTTTGTCCATCCGTTTGCCGTCAATCAGTGAACGCGGGCGAGCAGTAGGAATATGTTTg                                                                                                                                                             <  2:143264/251‑1 (MQ=255)                                                                                         gATAGCGCCGCTTGGGCAGGTCGCCACGCAGCTGGGATTGAGGCAGTGTTCGCACAGGCGCGGCAGGTACATCATGAAGGTGTTTTCAAACTGCCCGTACATCTCCTTTTGCATGGCCTCGAAGTTGCGGTCGTGGGCACGTTTTTCGAACTCACCGCTCAGCAGTTCTTCCCAGTTTGGCCCCCAGATCACTTTGTCCATCCGTTTGCCGTCAATCAGTGAACGCGGGCGAGCAGTAGGAATATGTTTGc                                                                                                                                                            <  1:32148/251‑1 (MQ=255)                                                                                               gccgcTTGGGCAGGTCGCCACGCAGCTGGGATTGAGGCAGTGTTCGCACAGGCGCGGCAGGTACATCATGAAGGGGTTTTCAAACTGCCCGTACATCTCCTTTTGCATGGCCTCGAAGTTGCGGTCGTGGGCACGTTTTTCGAACTCACCGCTCAGCAGTTCTTCCCAGTTTGGCCCCCAGATCACTTTGTCCATCCGTTTGCCGTCAATCAGTGAACGCGGGCGAGCAGTAGGAATATGTTTGCCTTcct                                                                                                                                                      >  2:228266/1‑250 (MQ=255)                                                                                                  gcTTGGGCAGGTCGCCACGCAGCTGGGATTGAGGCAGTGTTCGCACAGGCGCGGCAGGTACATCATGAAGGTGTTTTCAAACTGCCCGTACATCTCCTTTTGCATGGCCTCGAAGTTGCGGTCGTGGGCACGTTTTTCGAACTCACCGCTCAGCAGTTCTTCCCAGTTTGGCCCCCAGATCACTTTGTCCATCCGTTTGCCGTCAATCAGTGAACGCGGGCGAGCAGTAGGAATATGTTTGCCTTCCGGTg                                                                                                                                                   >  2:105918/1‑251 (MQ=255)                                                                                                  gcTTGGGCAGGTCGCCACGCAGCTGGGATTGAGGCAGTGTTCGCACAGGCGCGGCAGGTACATCATGAAGGTGTTTTCAAACTGCCCGTACATCTCCTTTTGCATGGCCTCGAAGTTGCGGTCGTGGGCACGTTTTTCGAACTCACCGCTCAGCAGTTCTTCCCAGTTTGGCCCCCAGATCACTTTGTCCATCCGTTTGCCGTCAATCAGTGAACGCGGGCGAGCAGTAGGAATATGTTTGCCTTCCGGTg                                                                                                                                                   >  1:161405/1‑251 (MQ=255)                                                                                                      gggCAGGTCGCCACGCAGCTGGGATTGAGGCAGTGTTCGCACAGGCGCGGCAGGTACATCATGAAGGTGTTTTCAAACTGCCCGTACATCTCCTTTTGCATGGCCTCGAAGTTGCGGTCGTGGGCACGTTTTTCGAACTCACCGCTCAGCAGTTCTTCCCAGTTTGGCCCCCAGATCACTTTGTCCATCCGTTTGCCGTCAATCAGTGAACGCGGGCGAGCAGTAGGAATATGTTTGCCTTCCGGTGCGCt                                                                                                                                               <  1:293529/251‑1 (MQ=255)                                                                                                                     cAGCTGGGATTGAGGCCGTGTTCGCACAGGCGCGGCAGGTACATCATGTAGGTGTTTTCAAACTGCCCGTACATCTCCTTTTGCATGGCCTCGAAGCTGCGGTCGTGCGCACGTTTTTTGAACTCACCGCTCAGCAGTTACTCCCCTTTTGGCCCCCAGATCACTTTTTCCATCCGTTTGCCGTCAATCAGTCAACGCGGGCGAGCAGTAGGAATCTGTTTGCCTTCCTGTGCGCTATGCAAATGTTCGtt                                                                                                                                >  2:212555/1‑250 (MQ=255)                                                                                                                     cAGCTGGGATTGAGGCAGTGTTCGCACAGGCGCGGCAGGTACATCATGAAGGTGTTTTCAAACTGCCCGTACATCTCCTTTTGCATGGCCTCGAAGTTGCGGTCGTGGGCACGTTTTTCGAACTCACCGCTCAGCAGTTCTTCCCAGTTTGGCCCCCAGATCACTTTGTCCATCCGTTTGCCGTCAATCAGTGAACGCGGGCGAGCAGTAGGAATATGTTTGCCTTCCGGTGCGCTATGCAAATGTTCGTa                                                                                                                                <  1:188775/251‑1 (MQ=255)                                                                                                                         tGGGATTGAGGCAGTGTTCGCACAGGCGCGGCAGGTACATCATGAAGGTGTTTTCAAACTGCCCGTACATCTCCTTTTGCATGGCCTCGAAGTTGCGGTCGTGGGCACGTTTTTCGAACTCACCGCTCAGCAGTTCTTCCCAGTTTGGCCCCCAGATCACTTTGTCCATCCGTTTGCCGTCAATCAGTGAACGCGGGCGAGCAGTAGGAata                                                                                                                                                                   >  1:37921/1‑212 (MQ=255)                                                                                                                         tGGGATTGAGGCAGTGTTCGCACAGGCGCGGCAGGTACATCATGAAGGTGTTTTCAAACTGCCCGTACATCTCCTTTTGCATGGCCTCGAAGTTGCGGTCGTGGGCACGTTTTTCGAACTCACCGCTCAGCAGTTCTTCCCAGTTTGGCCCCCAGATCACTTTGTCCATCCGTTTGCCGTCAATCAGTGAACGCGGGCGAGCAGTAGGAata                                                                                                                                                                   <  2:37921/212‑1 (MQ=255)                                                                                                                            gATTGAGGCAGTGTTCGCACAGGCGCGGCAGGTACATCATGAAGGTGTTTTCAAACTGCCCGTACATCTCCTTTTGCATGGCCTCGAAGTTGCGGTCGTGGGCACGTTTTTCGAACTCACCGCTCAGCAGTTCTTCCCAGTTTGGCCCCCAGATCACTTTGTCCATCCGTTTGCCGTCAATCAGTGAACGCGGGCGAGCAGTAGGAATATGTTTGCCTTCCGGTGCGCTATGCAAATGTTCGTAGTCGAAg                                                                                                                         <  2:161405/251‑1 (MQ=255)                                                                                                                                 aGGCAGTGTTCGCACAGGCGCGGCAGGTACATCATGAAGGTGTTTTCAAACTGCCCGTACATCTCCTTTTGCATGGCCTCGAAGTTGCGGTCGTGGGCACGTTTTTCGAACTCACCGCTCAGCAGTTCTTCCCAGTTTGGCCCCCAGATCACTTTGTCCATCCGTTTGCCGTCAATCAGTGAACGCGGGCGAGCAGTAGGAATATGTTTGCCTTCCGGTGCGCTATGCAAATGTTCGTAGTCGAAGGTGaa                                                                                                                    <  1:224615/251‑1 (MQ=255)                                                                                                                                      gtgtTCGCACAGGCGCGGCAGGTACATCATGAAGGTGTTTTCAAACTGCCCGTACATCTCCTTTTGCATGGCCTCGAAGTTGCGGTCGTGGGCACGTTTTTCGAACTCACCGCTCAGCAGTTCTTCCCAGTTTGGCCCCCAGATCACTTTGGCCATCCGTTTGCCGTCAATCAGTGAACGCGGGCGAGCAGTAGGAATATGTTTGCCTTCCGGTGCGCTATGCAAATGTTCGTAGTCGAAGGTGAAAGGtt                                                                                                               <  2:158987/251‑1 (MQ=255)                                                                                                                                        gtTCGCACAGGCGCGGCAGGTACATCATGAAGGTGTTTTCAAACTGCCCGTACATCTCCTTTTGCATGGCCTCGAAGTTGCGGTCGTGGGCACGTTTTTCGAACTCACCGCTCAGCAGTTCTTCCCAGTTTGGCCCCCAGATCACTTTGTCCATCCGTTTGCCGTCAATCAGTGAACGCGGGCGCGCAGTAGGAATATGTTTGCCTTCCGGTGCGCTATGCAAATGTTCGTAGTCGAAGGTGAAAGGTTCg                                                                                                             >  1:292791/1‑251 (MQ=255)                                                                                                                                               caGGCGCGGCAGGTACATCATGAAGGTGTTTTCAAACTGCCCGTACATCTCCTTTTGCATGGCCTCGAAGTTGCGGTCGTGGGCACGTTTTTCGAACTCACCGCTCAGCAGTTCTTCCCAGTTTGGCCCCCAGATCACTTTGTCCATCCGTTTGCCGTCAATCAGTGAACGCGGGCGAGCAGTAGGAATATGTTTGCCTTCCGGTGCGCTATGCAAATGTTCGTAGTCGAAGGTGAAAGGTTCGTAGTAat                                                                                                      <  2:221639/251‑1 (MQ=255)                                                                                                                                                        cAGGTACATCATGAAGGTGTTTTCAAACTGCCCGTACATCTCCTTTTGCATGGCCTCGAAGTTGCGGTCGTGGGCACGTTTTTCGAACTCACCGCTCAGCAGTTCTTCCCAGTTTGGCCCCCAGATCACTTTGTCCATCCGTTTGCCGTCAATCAGTGAACGCGGGCGAGCAGTAGGAATATGTTTGCCTTCCGGTGCGCTATGCAAATGTTCGTAGTCGAAGGTGAAAGGTTCGTAGTAATCATCAATCt                                                                                             >  1:319185/1‑251 (MQ=255)                                                                                                                                                                 catGAAGGTGTTTTCAAACTGCCCGTACATCTCCTTTTGCATGGCCTCGAAGTTGCGGTCGTGGGCACGTTTTTCGAACTCACCGCTCAGCAGTTCTTCCCAGTTTGGCCCCCAGATCACTTTGTCCATCCGTTTGCCGTCAATCAGTGAACGCGGGCGAGCAGTAGGAATATGTTTGCCTTCCGGTGCGCTATGCAAATGTTCGTAGTCGAAGGTGAAAGGTTCGTAGTAATCATCAATCTGCGGcacca                                                                                    <  1:311127/251‑1 (MQ=255)                                                                                                                                                                                 aaCTGCCCGTACATCTCCTTTTGCATGGCCTCGAAGTTGCGGTCGTGGGCACGTTTTTCGAACTCACCGCTCAGCAGTTCTTCCCAGTTTGGCCCCCAGATCACTTTGTCCATCCGTTTGCCGTCAATCAGTGAACGCGGGCGAGCAGTAGGAATATGTTTGCCTTCCGGTGCGCTATGCAAATGTTCGTAGTCGAAGGTGAAAGGTTCGTAGTAATCATCAATCTGCGGCACCACCGGGTTGGCGa                                                                        >  2:31734/1‑247 (MQ=255)                                                                                                                                                                                 aaCTGCCCGTACATCTCCTTTTGCATGGCCTCGAAGTTGCGGTCGTGGGCACGTTTTTCGAACTCACCGCTCAGCAGTTCTTCCCAGTTTGGCCCCCAGATCACTTTGTCCATCCGTTTGCCGTCAATCAGTGAACGCGGGCGAGCAGTAGGAATATGTTTGCCTTCCGGTGCGCGATGCAAATGTTCGTAGTCGAAGGTGAAAGGTTCGTAGTAATCATCAATCTGCGGCACCACCGGGTTGGCGAAGAt                                                                    <  2:244033/251‑1 (MQ=255)                                                                                                                                                                                       ccGTACATCTCCTTTTGCATGGCCTCGAAGTTGCGGTCGTGGGCACGTTTTTCGAACTCACCGCTCAGCAGTTCTTCCCAGTTTGGCCCCCAGATCACTTTGTCCATCCGCTTGCCGTCAATCAGTGAACGCGGGCGAGCAGTAGGAATATGTTTGCCTTCCCGTGCGCTATGCAAATGTTCGTAGTCGAAGGTGAAAGGCTCGTAGTAATCATCAATCTGCGGCACCACCGGGTTGGCGAAGATTTTGGt                                                              >  1:46111/1‑251 (MQ=255)                                                                                                                                                                                        cGTACATCTCCTTTTGCATGGCCTCGAAGTTGCGGTCGTGGGCACGTTTTTCGAACTCACCGCTCAGCAGTTCTTCCCAGTTTGGCCCCCAGATCACTTTGTCCATCCGTTTGCCGTCAATCAGTGAACGCGGGCGAGCAGTAGGAATATGTTTGCCTTCCGGTGCGCTATGCAAATGTTCGTAGTCGAAGGTGAAAGGTTCGTAGTAATCATCAATCTGCGGCACCACCGGGTTGGCGAAGATTTTGGtt                                                             >  1:345003/1‑251 (MQ=255)                                                                                                                                                                                              tctcCTTTTGCATGGCCTCGAAGTTGCGGTCGTGGGCACGTTTTTCGAACTCACCGCTCAGCAGTTCTTCCCAGTTTGGCCCCCAGATCACTTTGTCCATCCGTTTGCCGTCAATCAGTGAACGCGGGCGAGCAGTAGGAATATGTTTGCCTTCCGGTGCGCTATGCAAATGTTCGTAGTCg                                                                                                                            <  1:165652/182‑1 (MQ=255)                                                                                                                                                                                              tctcCTTTTGCATGGCCTCGAAGTTGCGGTCGTGGGCACGTTTTTCGAACTCACCGCTCAGCAGTTCTTCCCAGTTTGGCCCCCAGATCACTTTGTCCATCCGTTTGCCGTCAATCAGTGAACGCGGGCGAGCAGTAGGAATATGTTTGCCTTCCGGTGCGCTATGCAAATGTTCGTAGTCg                                                                                                                            >  2:165652/1‑182 (MQ=255)                                                                                                                                                                                              tctacTTTTGCATGGCCTCGAAGTTGCGGCCGTGGGCACGTTTTTCGAACTCACCGCTCAGCAGTTCTTCCCAGTTTGGCTCCCAGATCACTTTGTCCATCCGTTTGCCGTCAATCAGTGAACACGGGCGAGCAGTAGGAATATGTTTGCCTTCCGGTGCGCTATGCAAATGTTCGGAGTCGAAGGTGAAAGGATCGTAGTGATCATCAATCTGCGGCACCACCGGGTTGGCGACGATTTTGGTTATTACg                                                       >  2:154156/5‑251 (MQ=255)                                                                                                                                                                                              tcncCTTTTGCATGGTCTCGAAGTTGCGGTCGTGGGCACGTTTTTCGAACTCAACGCTCAGCAGTTCTTCCCAGTTTGGCCCCCAGATCACTTTGTCCATCCGTTTGCCGTCAATCAGTGAACGCGGGCGAGCAGTAGGAATATGTTTGCCTTCCGGTGCGCTATGCAAATGTTCGTAGTCGAAGGTGAAAGGTTCTTAGTAATCATCAATCTGCGGCACCTCCGGGTTGGCGAAGATTTTGGTTATTAc                                                        <  2:413175/250‑1 (MQ=255)                                                                                                                                                                                                            gCCTCGAAGTTGCGGTCGTGGGCACGTTTTTCGAACTCACCGCTCAGCAGTTCTTCCCAGTTTGGCCCCCAGATCACTTTGTCCATCCGTTTGCCGTCAATCAGTGAACGCGGGCGAGCAGTAGGAATATGTTTGCCTTCCGGTGCGCTATGCAAATGTTCGTAGTCGAAGGTGAAAGGTTCGTAGTAATCATCAATCTGCGGCACCACCGGGTTGGCGAAGATTTTGGTTATTACGCCCATCTTGTTGcc                                         <  2:345003/251‑1 (MQ=255)                                                                                                                                                                                                                     ttGCGGTCGTGGGCACGTTTTTCGAACTCACCGCTCAGCAGTTCTTCCCAGTTTGGCCCCCAGATCACTTTGTCCATCCGTTTGCCGTCAATCAGTGAACGCGGGCGAGCAGTAGGAATATGTTTGCCTTCCGGTGCGCTATGCAAATGTTCGTAGTCGAAGGTGAAAGGTTCGTAGTAATCATCAATCTGCGGCACCACCGGGTTGGCGAAGATTTTGGTTATTACGCCCATCTTGTTGCCCAGACGCgg                                >  2:174118/1‑251 (MQ=255)                                                                                                                                                                                                                                ggCACGTTTTTCGAACTCACCGCTCAGCAGTTCTTCCCAGTTTGGCCCCCAGATCACTTTGTCCATCCGTTTGCCGTCAATCAGTGAACGCGGGCGAGCAGTAGGAATATGTTTGCCTTCCGGTGCGCTATGCAAATGTTCGTAGTCGAAGGTGAAAGGTTCGTAGTAATCATCAATCTGCGGCACCACCGGGTTGGCGAAGATTTTGGTTATTACGCCCATCTTGTTGCCCAGACGCGGGCGTATCTTGc                     >  2:83254/1‑251 (MQ=255)                                                                                                                                                                                                                                      tttttCGAACTCACCGCTCAGCAGTTCTTCCCAGTTTGGCCCCCAGATCACTTTGTCCATCCGTTTGCCGTCAATCAGTGAACGCGGGCGAGCAGTAGGAATATGTTTGCCTTCCGGTGCGCTATGCAAATGTTCGTAGTCGAAGGTGAAAGGTTCGTAGTAATCATCAATCTGCGGCACCACCGGGTTGGCGAAGATTTTGGTTATTACGCCCATCTTGTTGCCCAGACGCGGGCGTATCTTGCCATTc                >  2:350706/1‑250 (MQ=255)                                                                                                                                                                                                                                            gAACTCACCGCTCAGCAGTTCTTCCCAGTTTGGCCCCCAGATCACTTTGTCCATCCGTTTGCCGTCAATCAGTGAACGCGGGCGAGCAGTAGGAATATGTTTGCCTTCCGGTGCGCTATGCAAATGTTCGTAGTCGAAGGTGAAAGGTTCGTAGTAATCATCAATCTGCGGCACCACCGGGTTGGCGAAGATTTTGGTTATTACGCCCATCTTGTTGCCCAGACGCGGGCGTATCTTGCCATTCACATCAc         >  2:214732/1‑251 (MQ=255)                                                                                                                                                                                                                                                   ccGCTCAGCAGTTCTTCCCAGTTTGGCCCCCAGATCACTTTGTCCATCCGTTTGCCGTCAATCAGTGAACGCGGGCGAGCAGTAGGAATATGTTTGCCTTCCGGTGCGCTATGCAAATGTTCGTAGTCGAAGGTGAAAGGTTCGTAGTAATCATCAATCTGCGGCACCACCGGGTTGGCGAAGATTTTGGTTATTACGCCCATCTTGTTGCCCAGACGCGGGCGTATCTTGCCATTCACATCACGCACCCa  <  2:42155/251‑1 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                       TTTTGTACGGACAACCGCTTATGCACAAACGCCAGCCACGGCATTTATCCTGATCAATCAGCACAATGCCGTCTTCTTCGCGTTTGTAGATAGCGCCGCTTGGGCAGGTCGCCACGCAGCTGGGATTGAGGCAGTGTTCGCACAGGCGCGGCAGGTACATCATGAAGGTGTTTTCAAACTGCCCGTACATCTCCTTTTGCATGGCCTCGAAGTTGCGGTCGCGGGCACGTTTTTCGAACTCACCGCCCAGCAGTTCTTCCCAGTTTGGCCCCCAGATCACTTTGTCCATCCGTTTGCCGTCAATCAGTGAACGCGGGCGAGCAGTAGGAATATGTTTGCCTTCCGGTGCGCTATGCAAATGTTCGTAGTCGAAGGTGAAAGGTTCGTAGTAATCATCAATCTGCGGCACCACCGGGTTGGCGAAGATTTTGGTTATTACGCCCATCTTGTTGCCCAGACGCGGGCGTATCTTGCCATTCACATCACGCACCCA  >  NC_000913/1538166‑1538658

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑