Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,095,879	G→A	D13N (GAT→AAT)	ycdU →	uncharacterized protein YcdU

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,095,879	0	G	A	100.0%	93.1 / NA	27	D13N (GAT→AAT)	ycdU	uncharacterized protein YcdU
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (12/15);  total (12/15)

AAAGAAAATCTCTCATTACACTTAACTCATACGGTGAAATGGACTGAATCTCTCGATACATTTCTGAATATGCCAACCCCGGTAGCTTTCCTTGAAATAAGTAATAAGCCCTATTTGGGGAATATGTTAAATGATTTTGCTGGCGTGGATCAGCAACGAGTTATGCATTGTCGTAAAGCATTTAGTGATGCAAAGGTATTCAAATGATTCCTGATTATTTAACTTTTATTCGCTTTCAGGATAAACGAAATCTGATATACATTTATGCTATTGGACTTATTCTGATAGGCTTTTATTGGAAGAATGCAGGGTTTACTTTTCCATCAGAGGATATTGGTGTAGTTAGTGGGATTCTGGCTCTGGTGCTGTATAATTTTATTTTTGATCTCAAGGCGTACTGGGCTTATAAATGCGTCACGAAGAATATCGATTTTTCGTGGTTTAAGAAAAAGCAGAACCACAAAATAGAATTATTTCTTA  >  NC_000913/1095640‑1096119                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                 aaaGAAAATCTCTCATTACACTTAACTCATACGGTGAAATGGACTGAATCTCTCGATACATTTCTGAATATGCCAACCCCGGTAGCTTTCCTTGAAATAAGTAATAAGCCCTATTTGGGGAATATGTTAAATGAGTTTGCTGGCGTGGATCAGCAACGAGTTATGCATTGTCGTAAAGCATTTAGTGATGCAAAGGTATTCAAATGATTCCTGATTATTTAACTTTTATTCGCTTTCAGAATAAACGAAAt                                                                                                                                                                                                                                       <  1:273119/251‑1 (MQ=255)      aaaTCTCTCAGTACACTTAACTCATACGGTGAAATGGACTGAATCTCTCGATACATTTCTGAATATGCCAACCCCGGTAGCTTTCCTTGAAATAAGTAATAAGCCCTATTTGGGGAATATGTTAAATGATTTTGCTGGCGTGGATCAGCAACGAGTTATGCATTGTCGTAAAGCATTTAGTGATGCAAAGGTATTCAAATGATTCCTGATTATTTAACTTTTATTCGCTTTCAGAATAAACGAAATCTGat                                                                                                                                                                                                                                  <  2:265063/251‑1 (MQ=255)            ctcATTACACTTAACTCATACGGTGAAATGGACTGAATCTCTCGATACATTTCTGAATATGCCAACCCCGGTAGCTTTCCTTGAAATAAGTAATAAGCCCTATTTGGGGAATATGTTAAATGATTTTGCTGGCGTGTATCAGCAACGAGTTATGCATTGTCGTAAAGCATTTAGTGATGCAAAGGTATTCAAATGATGCCTGATTATTTAACTTTTATTCGCTTTCAGAATAAACGAAATCTGAtata                                                                                                                                                                                                                               <  1:264858/248‑1 (MQ=255)                    acTTAACTCATACGGTGAAATGGACTGAATCTCTCGATACATTTCTGAATATGCCAACCCCGGTAGCTTTCCTTGAAATAAGTAATAAGCCCTATTTGGGGAATATGTTAAATGATTTTGCTGGCGTGGATCAGCAACGAGTTATGCATTGTCGTAAAGCATTTAGTGATGCAAAGGTATTCAAATGATTCCTGATTATTTAACTTTTATTCGCTTTCAGAATAAACGAAATCTGATATACATTTATGCTa                                                                                                                                                                                                                    <  1:242834/251‑1 (MQ=255)                       tAACTCATACGGTGAAATGGACTGAATCTCTCGATACATTTCTGAATATGCCAACCCCGGTAGCTTTCCTTGAAATAAGTAATAAGCCCTATTTGGGGAATATGTTAAATGATTTTGCTGGCGTGGATCAGCAACGAGTTATGCATTGTCGTAAAGCATTTAGTGATGCAAAGGTATTCAAATGATTCCTGATTATTTAACTTTTATTCGCTTTCAGAATAAACGAAATCTGATATACATTTATGCTAtt                                                                                                                                                                                                                  <  1:111773/250‑1 (MQ=255)                            cATACGGTGAAATGGACTGAATCTCTCGATACATTTCTGAATATGCCAACCCCGGTAGCTTTCCTTGAAATAAGTAATAAGCCCTATTTGGGGAATATGTTAAATGATTTTGCTGGCGTGGATCAGCAACGAGTTATGCATTGTCGTAAAGCATTTAGTGATGCAAAGGTATTCAAATGATTCCTGATTATTTAACTTTTATTCGCTTTCAGAATAAACGAAATCTGATATACATTTATGCTATTGGACtt                                                                                                                                                                                                            >  1:352751/1‑251 (MQ=255)                                   tGAAATGGACTGAATCTCTCGATACATTTCTGAATATGCCAACCCCGGTAGCTTTCCTTGAAATAAGTAATAAGCCCTATTTGGGGAATATGTTAAATGCTTTTGCTGGCGTGGATCAGCAACGAGTTATGCATTGTCGTAAAGCATTTAGTGATGCAAAGGTATTCAAATGATTCCTGATTATTTAACTTTTATTCGCTTTCAGAATAAACGAAATCTGATATACATTTATGCTATTGGACTTATTCTg                                                                                                                                                                                                      >  1:444709/1‑250 (MQ=255)                                       aTGGACTGAATCTCTCGATACATTTCTGAATATGCCAACCCCGGTAGCTTTCCTTGAAATAAGTAATAAGCCCTATTTGGGGAATATGTTAAATGATTTTGCTGGCGTGGATCAGCAACGAGTTATGCATTGTCGTAAAGCATTTAGTGATGCAAAGGTATTCAAATGATTCCTGATTATTTAACTTTTATTCGCTTTCAGAATAAACGAAATCTGATATACATTTATGCTATTGGACTTATTCTGATAgg                                                                                                                                                                                                 <  1:120977/251‑1 (MQ=255)                                                      cGATACATTTCTGAATATGCCAACCCCGGTAGCTTTCCTTGAAATAAGTAATAAGCCCTATTTGGGGAATATGTTAAATGATTTTGCTGGCGTGGATCAGCAACGAGTTATGCATTGTCGTAAAGCATTTAGTGATGCAAAGGTATTCAAATGATTCCTGATTATTTAACTTTTATTCGCTTTCAGAATAAACGAAATCTGATATACATTTATGCTATTGGACTTATTCTGATAGGCTTTTATTGgaagaa                                                                                                                                                                                  <  1:235389/251‑1 (MQ=255)                                                                           aaCCCCGGTAGCTTTCCTTGAAATAAGTAATAAGCCCTATTTGGGGAATATGTTAAATGATTTTGCTGGCGTGGATCAGCAACGAGTTATGCATTGTCGTAAAGCATTTAGTGATGCAAAGGTATTCAAATGATTCCTGATTATTTAACTTTTATTCGCTTTCAGAATAAACGAAATCTGATATACATTTATGCTATTGGACTTATTCTGATAGGCTTTTATTGGAAGAATGCAGGGTTTACTTTTCCATc                                                                                                                                                             >  2:163262/1‑251 (MQ=255)                                                                              tccGGTAGCTTTCCTTGAAATAAGTAATAAGCCCTATTTGGGGAATATGTTAAATGATTTTGCTGGCCTGGATCAGCAACGAGTTATGCATTGTCGTAAAGCATTTAGTGATTCAAAGGTATTCAAATGATTCCTGATTATTTAACTTTTATTCGCTTTCAGAATAAACGATCTCTGATATACCTTTATGCTATTGAACTTATTATGATAGGCTTTTATTGGACGAATGTGGGGTTTCATTTTCCATc                                                                                                                                                             >  2:226232/2‑248 (MQ=255)                                                                                                              ccTATTTGGGGAATATGTTAAATGATTTTGCTGGCGTGGATCAGCAACGAGTTATGCATTGTCGTAAAGCATTTAGTGATGCAAAGGTATTCAAATGATTCCTGATTATTTAACTTTTATTCGCTTTCAGAATAAACGAAATCTGATATACATTTATGCTATTGGACTTATTCTGATAGGCTTTTATTGGAAGAATGCAGGGTTTACTTTTCCATCAGAGGATATTGGTGTAGTTAGTGGGATTCTGGctc                                                                                                                          >  1:26711/1‑251 (MQ=255)                                                                                                              ccTATTTGGGGAATATGTTAAATGATTTTGCTGGCGTGGATCAGCAACGAGTTATGCATTGTCGTAAAGCATTTAGTGATGCAAAGGTATTCAAATGATTCCTGATTATTTAACTTTTATTCGCTTTCAGAATAAACGAAATCTGATATACATTTATGCTATTGGACTTATTCTGATAGGCTTTTATTGGAAGAATGCAGGGTTTACTTTTCCATCAGAGGATATTGGTGTAGTTAGTGGGATTCTGGctc                                                                                                                          <  2:13811/251‑1 (MQ=255)                                                                                                                     ggggAATATGTTAAATGATTTTGCTGGCGTGGATCAGCAACGAGTTATGCATTGTCGTAAAGCATTTAGTGATGCAAAGGTATTCAAATGATTCCTGATTATTTAACTTTTATTCGCTTTCAGAATAAACGAAATCTGATATACATTTATGCTATTGGACTTATTCTGATAGGCTTTTATTGGAAGAATGCAGGGTTTACTTTTCCATCAGAGGATATTGGTGTAGTTAGTGGGATTCTGGCTCTGGTGCt                                                                                                                   >  2:328858/1‑251 (MQ=255)                                                                                                                       ggAATATGTTAAATGATTTTGCTGGCGTGGATCAGCAACGAGTTATGCATTGTCGTAAAGCATTTAGTGATGCAAAGGTATTCAAATGATTCCTGATTATTTAACTTTTATTCGCTTTCAGAATAAACGAAATCTGATATACATTTATGCTATTGGACTTATTCTGATAGGCTTTTATTGGAAGAATGCAGGGTTTACTTTGCCATCAGAGGATATTGGTGTAGTTAGTGGGATTCTGGCTCTGGTGCTGt                                                                                                                 <  1:262822/251‑1 (MQ=255)                                                                                                                                     gATTTTGCTGGCGTGGATCAGCAACGAGTTATGCATTGTCGTAAAGCATTTAGTGATGCAAAGGTATTCAAATGATTCCTGATTATTTAACTTTTATTCGCTTTCAGAATAAACGAAATCTGATATACATTTATGCTATTGGACTTATTCTGATAGGCTTTTATTGGAAGAATGCAGGGtt                                                                                                                                                                         >  1:210392/1‑181 (MQ=255)                                                                                                                                     gATTTTGCTGGCGTGGATCAGCAACGAGTTATGCATTGTCGTAAAGCATTTAGTGATGCAAAGGTATTCAAATGATTCCTGATTATTTAACTTTTATTCGCTTTCAGAATAAACGAAATCTGATATACATTTATGCTATTGGACTTATTCTGATAGGCTTTTATTGGAAGAATGCAGGGtt                                                                                                                                                                         <  2:210392/181‑1 (MQ=255)                                                                                                                                              ggCGTGGATCAGCAACGAGTTATGCATTGTCGTAAAGCATTTAGTGATGCAAAGGTATTCAAATGATTCCTGATTATTTAACTTTTATTCGCTTTCAGAATAAACGAAATCTGATATACATTTATGCTATTGGACTTATTCTGATAGGCTTTTATTGGAAGAATGCAGGGTTTACTTTTCCATCAGAGGATATTGGTGTAGTTAGTGGGATTCTGGCTCTGGTGCTGTATAATTTTATTTTTGATCTCa                                                                                            <  1:163262/249‑1 (MQ=255)                                                                                                                                              ggCGTGGATCAGCAACGAGTTATGCATTGTCGTAAAGCATTTAGTGATGCAAAGGTATTCAAATGATTCCTGATTATTTAACTTTTATTCGCTTTCAGAATAAACGAAATCTGATATACATTTATGCTATTGGACTTATTCTGATAGGCTTTTATTGGAAGAATGCAGGGTTTACTTTTCCATCAGAGGATATTGGTGTAGTTAGTGGGATTCTGGCTCTGGTGCTGTATAATTTTATTTTTGATCTCa                                                                                            >  1:73824/1‑249 (MQ=255)                                                                                                                                                                      cATTGTCGTAAAGCATTTAGTGATGCAAAGGTATTCAAATGATTCCTGATTATTTAACTTTTATTCGCTTTCAGAATAAACGAAATCTGATATACATTTATGCTATTGGACTTATTCTGATAGGCTTTTATTGGAAGAATGCAGGGTTTACTTTTCCATCAGAGGATATTGGTGTAGTTAGTGGGATTCTGGCTCTGGTGCTGTATAATTTTATTTTTGATCTCAAGGCGTACTGGGCTTATAAATGCGTc                                                                  <  1:233910/251‑1 (MQ=255)                                                                                                                                                                           tCGTAAAGCATTTAGGGATGCAAAGGTATTCAAATGATTACTGATTATTTAACTTTTATTCGCTTTCAGAATAAACGAAATCTGATATACATTTATGCTATTGGACTTATTCTGATAGGCTTTTATTGGAAGAATGCAGGGTTTACTTTTCCATCAGAGGATATTGGTGTAGTTAGTGGGATTCTGGCTCTGGTGCTGTATCATTTTATTTTTGATCTCAAGGCGTACTGGGCTTATAAATGCGTCACga                                                              >  2:454989/1‑250 (MQ=255)                                                                                                                                                                             gTAAAGCATTTAGTGATGCAAAGGTATTCAAATGATTCCTGATTATTTAACTTTTATTCGCTTTCAGAATAAACGAAATCTGATATACATTTATGCTATTGGACTTATTCTGATAGGCTTTTATTGGAAGAATGCAg                                                                                                                                                                             >  2:127736/1‑137 (MQ=255)                                                                                                                                                                             gTAAAGCATTTAGTGATGCAAAGGTATTCAAATGATTCCTGATTATTTAACTTTTATTCGCTTTCAGAATAAACGAAATCTGATATACATTTATGCTATTGGACTTATTCTGATAGGCTTTTATTGGAAGAATGCAg                                                                                                                                                                             <  1:127736/137‑1 (MQ=255)                                                                                                                                                                                                                                 tttATTCGCTTTCAGAATAAACGAAATCTGATATACATTTATGCTATTGGACTTATTCTGATAGGCTTTTATTGGAAGAATGCAGGGTTTACTTTTCCATCAGCGGATATTGGTGTAGTTAGTGGGATTCTGGCTCTGGTGCTGTATAATTTTATTTTTGATCTCAAGGCGTACTGGGCTTATAAATGCGTCAc                                                                >  1:183271/1‑194 (MQ=255)                                                                                                                                                                                                                                 tttATTCGCTTTCAGAATAAACGAAATCTGATATACATTTATGCTATTGGACTTATTCTGATAGGCTTTTATTGGAAGAATGCAGGGTTTACTTTTCCATCAGAGGATATTGGTGTAGTTAGTGGGATTCTGGCTCTGGTGCTGTATAATTTGATTTTTGATCTCAAGGCGTACTGGGCTTATAAATGCGTCAc                                                                <  2:183271/194‑1 (MQ=255)                                                                                                                                                                                                                                     ttCGCTTTCAGAATAAACGAAATCTGATATACATTTATGCTATTGGACTTATTCTGATAGGCTTTTATTGGAAGAATGCAGGGTTTACTTTTCCATCAGAGGATATTGGTGTAGTTAGTGGGATTCTGGCTCTGGTGCTGTATAATTTTATTTTTGATCTCAAGGCGTACTGGGCTTATAAATGCGTCACGAAGAATATCGATTTTTCGTGGTTTAAGAAAAAGCAGAACCACAAAATAGAATTATTTCtt   >  1:315365/1‑251 (MQ=255)                                                                                                                                                                                                                                       cGCTTTCAGAATAAACGAAATCTGATATACATTTATGCTATTGGACTTATTCTGATAGGCTTTTATTGGAAGAATGCAGGGTTTACTTTTCCATCAGAGGATATTGGTGTAGTTAGTGGGATTCTGGCTCTGGTGCTGTATAATTTTATTTTTGATCTCAAGGCGTACTGGGCTTATAAATGCGTCACGAAGAATATCGATTTTTCGTGGTTTAAGAAAAAGCAGAACCACAAAATAGAATTATTTCTTa  <  2:26711/250‑1 (MQ=255)                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                 AAAGAAAATCTCTCATTACACTTAACTCATACGGTGAAATGGACTGAATCTCTCGATACATTTCTGAATATGCCAACCCCGGTAGCTTTCCTTGAAATAAGTAATAAGCCCTATTTGGGGAATATGTTAAATGATTTTGCTGGCGTGGATCAGCAACGAGTTATGCATTGTCGTAAAGCATTTAGTGATGCAAAGGTATTCAAATGATTCCTGATTATTTAACTTTTATTCGCTTTCAGGATAAACGAAATCTGATATACATTTATGCTATTGGACTTATTCTGATAGGCTTTTATTGGAAGAATGCAGGGTTTACTTTTCCATCAGAGGATATTGGTGTAGTTAGTGGGATTCTGGCTCTGGTGCTGTATAATTTTATTTTTGATCTCAAGGCGTACTGGGCTTATAAATGCGTCACGAAGAATATCGATTTTTCGTGGTTTAAGAAAAAGCAGAACCACAAAATAGAATTATTTCTTA  >  NC_000913/1095640‑1096119

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑