Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,837,658	G→A	W149* (TGG→TGA)	ynjC →	putative ABC transporter membrane subunit YnjC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,837,658	0	G	A	100.0%	232.3 / NA	67	W149* (TGG→TGA)	ynjC	putative ABC transporter membrane subunit YnjC
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (25/42);  total (25/42)

GGCATTGTTGATTGCCCTGCTGGTGATTGTGGCGCTGTGGCCTGGGCCGAAATGGCAGCGTATGTGCGCCCGTCTGCCGTGGCTGCTCGCCATTCCCCATGTGGCTTTTGCCACCAGCGCCCTTCTGCTCTTTGCTGACGGAGGGCTGCTTTATGACTATTTCCCGTATTTCACTCCGCCAATGGACCGATTTGGCATCGGTCTGGGCCTCACCCTTGCAGTGAAAGAAAGCGCATTTCTGCTGTGGATCTTAGCAGCAGTATTGAGCGAAAAATGGCTGTTGCAGCAGGTCATTGTGCTGGATTCGCTGGGCTACAGCCGCTGGCAATGCCTGAACTGGCTGCTGTTGCCCTCCGTCGCGCCTGCGCTGGCAATGGCGATGCTGGCGATTGTTGCCTGGTCGCTGTCGGTCGTGGATGTGGCAATTATTCTCGGGCCAGGTAATCCCCCGACGCTGGCGGTAATTAGCTGGCAGTGGTTAACCC  >  NC_000913/1837412‑1837896                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                               ggCATTGTTGATTGCCCTGCTGGTGGTTGTGGCGCTGTGGCCTGGGCCGAAATGGCAGCGTATGTGCGCCCGTCGGCCGTGGCTGCTCGCCATTCCCCATGTGGCTTTTGCCTCCAGCGCCCTTCTGCGCTTTGCTGACGGAGGGCTGCTTTATGACTATTTCCCGTATTTCACTCCGCCAATGGACCGAGTTGGCATCGGTCTGGGCCTCACCCTTGCAGTGAAAGAAAGCGCATTTCTGCTGTGAATCt                                                                                                                                                                                                                                            <  2:203113/251‑1 (MQ=255)  gCATTGTTGATTGCCCTGCTGGTGATTGTGGCGCTGTGGCCTGGGCCGAAATGGCAGCGTATGTGCGCCCGTCTGCCGTGGCTGCTCGCCATTCCCCATGTGGCTTTTGCCCCCAGCGCCCTTCTGCTCTTTGCTGACGGAGGGCTGCTTTATGACTATTTCCCGTATTTCACTCCGCCAATGGACCGATTTGGCATCTGTCTGGGCCTCACCCTTGCAGTGAAAGAAAGCGCATTTCTGCTGTGAATCtt                                                                                                                                                                                                                                           <  1:445075/251‑1 (MQ=255)       gttgATTGCCCTGCTGGTGATTGTGGCGCTGTGGCCTGGGCCGAAATGGCAGCGTATGTGCGCCCGTCTGCCGTGGCTGCTCGCCATTCCCCATGTGGCTTTTGCCACCAGCGCCCTTCTGCTCTTTGCTGACGGAGGGCTGCTTTATGACTATTTCCCGTATTTCACTCCGCCAATGGACCGATTTGGCATCGGTCTGGGCCTCACCCTTGCAGTGAAAGAAAGCGCATTTCTGCTGTGAATCTTagca                                                                                                                                                                                                                                       <  1:388488/250‑1 (MQ=255)         tgATTGCCCTGCTGGTGATTGTGGCGCTGTGGCCTGGGCCGAAATGGCAGCGTATGTGCGCCCGTCTGCCGTGGCTGCTCGCCATTCCCCATGTGGCTTTTGCCACCAGCGCCCTTCTGCTCTTTGCTGACGGAGGGCTGCTTTATGACTATTTCCCGTATTTCACTCCGCCAATGGACCGATTTGGCATCGGTCTGGGCCTCACCCTTGCAGTGAAAGAAAGCGCATTTCTGCTGTGAATCTTagcagc                                                                                                                                                                                                                                     <  2:378728/250‑1 (MQ=255)              gaccTGCTGGTGATTGTGGCGCGGGTGCCTGGGCCGAAATGGCAGCGTATGTGCGCCCGTCTGCCGTGGCTGCTCTCCATTCCCCATGTGGCGTTTGCCACCAGCGCGCTTCTGCTCTTTGCGTACGGAGGGCTGCTTTATGACTATTTCCCGTATTTCACTCCGCCAATGGACCGATTTGGCATCGGTCTGGGCCTCACCCTTGCAGTGAAATAAAGCGCATTTCTGCTGTTAATCTTAGCAGCAGTAt                                                                                                                                                                                                                                <  2:29519/248‑1 (MQ=255)                   gctgGTGATTGTGGCGCTGTGGCCTGGGCCGAAATGGCAGCGTATGTGCGCCCGTCTGCCGTGGCTGCTCGCCATTCCCCATGTGGCTTTTGCCACCAGCGCCCTTCTGCTCTTTGCTGACGGAGGGCTGCTTTATGACTATTTCCCGTATTTCACTCCGCCAATGGACCGATTTGGCATCGGTCTGGGCCTCACCCTTGCAGTGAAAGAAAGCGCATTTCTGCTGTGAATCTTAGCAGCAGTATTGAGc                                                                                                                                                                                                                           <  2:92297/250‑1 (MQ=255)                          aTTGTGGCGCTGTGGCCTGGGCCGAAATGGCAGCGTATGTGCGCCCGTCTGCCGTGGCTGCTCGCCATTCCCCATGTGGCTTTTGCCACCAGCGCCCTTCTGCTCTTTGCTGACGGAGGGCTGCTTTATGACTATTTCCCGTATTTCACTCCGCCAATGGACCGATTTGGCATCGGTCTGGGCCTCACCCTTGCAGTGAAAGAAAGCGCATTTCTGCTGTGAATCTTAGCAGCAGTATTGAGCGAAAAATg                                                                                                                                                                                                                   <  2:236418/251‑1 (MQ=255)                            tgtgGCGCTTTTGCCTGGGCCGACATGGCAGTGTATGTGCGCCCGTCTGCCGTGGCTGCTCGCCATTCCCCATGTGGCTTTTGCCACCAGCGCCCTTCTGCTCTTTGCTTACGGAGGGCTGCTTTATGACTATTTCCCGTATTTCACTCCGCCAATGGACCGATTTGGCATCTGTCTGGGCCTCACCCTTGCAGTGAAAGAAAGCGCATTTCTGCTGTGAATCTTAGCAGCAGTATTGAGCGAAAAATgg                                                                                                                                                                                                                  <  2:141513/250‑1 (MQ=255)                             gtgGCGCTGTGGCCTGGGCCGAAATGGCAGCGTATGTGCGCCCGTCTGCCGTGGCTGCTCGCCATTCCCCATGTGGCTTTTGCCACCAGCGCCCTTCTGCTCTTTGCTGACGGAGGGCTGCTTTATGACTATTTCCCGTATTTCACTCCGCCAATGGACCGATTTGGCATCGGTCTGGGCCTCACCCTTGCAGTGAAAGAAAGCGCATTTCTGCTGTGAATCTTAGCAGCAGTATTGAGCGAAAAATGGCt                                                                                                                                                                                                                >  1:371741/1‑251 (MQ=255)                               ggCNCTGTGGCCTGGGCCGAAATGGCAGCGTATGTGCGCCCGTCTGCCGTGGCTGCTCGCCATTCCCCATGTGGCTTTTGCCACCAGCGCCCTTCTGCTCTTTGCTGACGGAGGGCTGCTTTATGACTATTTCCCGTATTTCACTCCGCCAATGGACCGATTTGGCATCGGTCTGGGCCTCACCCTTGCAGTGAAAGAAAGCGCATTTCTGCTGTGAATCTTAGCAGCAGTATTGAGCGAAAAATGGCTGt                                                                                                                                                                                                              <  2:412872/251‑1 (MQ=255)                                    tgtgGCCTGGGCCGAAATGGCAGCGTATGTGCGCCCGTCTGCCGTGGCTGCTCGCCATTCCCCATGTGGCTTTTGCCACCAGCGCCCTTCTGCTCTTTGCTGACGGAGGGCTGCTTTATGACTATTTCCCGTATTTCATTCCGCCAATGGACCGATTTGGCATCGGTCTGGGCCTCACCCTTGCAGTGAAAGAAAGCGCATTTCTGCTGTGAATCTTAGCAGCAGTCTTGAGCGAAAAATGGCTGTTgc                                                                                                                                                                                                           >  2:319743/1‑249 (MQ=255)                                         ccTGGGCCGAAATGGCAGCGTATGTGCGCCCGTCTGCCGTGGCTGCTCGCCATTCCCCATGTGGCTTTTGCCACCAGCGCCCTTCTGCTCTTTGCTGACGGAGGGCTGCTTTATGACTATTTCCCGTATTTCACTCCGCCAATGGACCGATTTGGCATCGGTCTGGGCCTCACCCTTGCAGTGAAAGAAAGCGCATTTCTGCTGTGAATCTTAGCAGCAGTATTGAGCGAAAAATGGCTGTTGCAGCAGGt                                                                                                                                                                                                    <  2:10415/251‑1 (MQ=255)                                            gggCCGAAATGGCAGCGTATGTGCGCCCGTCTGCCGTGGCTGCTCGCCATTCCCCATGTGGCTTTTGCCACCAGCGCCCTTCTGCTCTTTGCTGACGGAGGGCTGCTTTATGACTATTTCCCGTATTTCACTCCGCCAATGGACCGATTTGGCATCGGTCTGGGCCTCACCCTTGCAGTGAAAGAAAGCGCATTTCTGCTGTGAATCTTAGCAGCAGTATTGAGCGAAAAATGGCTGTTGCAGCAGGTc                                                                                                                                                                                                   <  1:392173/249‑1 (MQ=255)                                              gCCGAAATGGCAGCGTATGTGCGCCCGTCTGCCGTGGCTGATCGCCATTCCCCATGTGGCTTTTGCCACCAGCGCCCTTCTGCTCTTTGCTGACGGAGGGCTGCTTTATGACTATTTCCCGTATTTCACTCCGCCAATGGACCGATTTGGGATCGGTCTGGGCCTCACCCTTGCAGTGAAAGAAAGCGCATTTCTGATGTGAATCTTAGCAGCAGTATTGAGCGAAAAATGGCTGTTGCAGCAGGGCAttt                                                                                                                                                                                               >  2:223510/1‑250 (MQ=255)                                                       gCAGCGTATGTGCGCCCGTCTGCCGTGGCTGCTCGCCATTCCCCATGTGGCTTTTGCCACCAGCGCCCTTCTGCTCTTTGCTGACGGAGGGCTGCTTTATGACTATTTCCCGTATTTCACTCCGCCAATGGACCGATTTGGCATCGGTCTGGGCCTCACCCTTGCAGTGAAAGAAAGCGCATTTCTGCTGTGAATCTTAGCAGCAGTATTGAGCGAAAAATGGCTGTTGCAGCAGGTCATTGTGCTGGAtt                                                                                                                                                                                      <  2:174815/251‑1 (MQ=255)                                                        cAGCGTATGTGCGCCCGTCTGCCGTGGCTGCTCGCCATTCCCCATGTGGCTTTTGCCACCAGCGCCCTTCTGCTCTTTGCTGACGGAGGGCTGCTTTATGACTATTTCCCGTATTTCACTCCGCCAATGGACCGATTTGGCATCGGTCTGGGCCTCACCCTTGCAGTGAAAGAAAGCGCATTTCTGCTGTGAATCTTAGCAGCAGTATTGAGCGAAAAATGGCTGTTGCAGCAGGTCATTGTGCTGGATTc                                                                                                                                                                                     <  1:371471/251‑1 (MQ=255)                                                                 tgCGCCCGTCTGCCGTGGCTGCTCGCCATTCCCCATGTGGCTTTTGCCACCAGCGCCCTTCTGCTCTTTGCTGACGGAGGGCTGCTTTATGACTATTTCCCGTATTTCACTCCGCCAATGGACCGATTTGGCATCGGTCTGGGCCTCACCCTTGCAGTGAAAGAAAGCGCATTTATGCTGTGAATCTTAGCAGCAGTATTGAGCGAAAAATGGCTGTTGCAGCAGGTCATTGTGCTGGATTCGCTGGGCt                                                                                                                                                                             >  1:312378/1‑250 (MQ=255)                                                                  gcgcCCGTCTGCCGTGGCTGCTCGCCATTCCCCATGTGGCTTTTGCCACCAGCGCCCTTCTGCTCTTTGCTGACGGAGGGCTGCTTTATGACTATTTCCCGTATTTCACTCCGCCAATGGACCGATTTGGCATCGGTCTGGGCCTCACCCTTGCAGTGAAAGAAAGCGCATTTCTGCTGTGAATCTTAGCAGCAGTATTGAGCGAAAAATGGCTGTTGCAGCAGGTCATTGTGCTGGATTCGCTGGGCTAc                                                                                                                                                                           <  1:261200/251‑1 (MQ=255)                                                                        gTCTGCCGTGGCTGCTCGCCATTCCCCATGTGGCTTTTGCCACCAGCGCCCTTCTGCTCTTTGCTGACGGAGGGCTGCTTTATGACTATTTCCCGTATTTCACTCCGCCAATGGACCGATTTGGCATCGGTCTGGGCCTCACCCTTGCAGTGAAAGAAAGCGCATTTCTGCTGTGAATCTTAGCAGCAGTATTGAGCGAAAAATGGCTGTTGCAGCAGGTCATTGTGCTGGATTCGCTGGGCTACAGCCGc                                                                                                                                                                     >  1:329694/1‑251 (MQ=255)                                                                            gCCGTGGCTGCTCGCCATTCCCCATGTGGCTTTTGCCACCAGCGCCCTTCTGCTCTTTGCTGACGGAGGGCTGCTTTATGACTATTTCCCGTATTTCACTCCGCCAATGGACCGATTTGGCATCGGTCTGGGCCTCACCCTTGCAGTGAAAGAAAGCGCATTTCTGCTGTGAATCTTAGCAGCAGTATTGAGCGAAAAATGGCTGTTGCAGCAGGTCATTGTGCTGGATTCGCTGGGCTACAGCCGCTGGc                                                                                                                                                                 <  1:363999/251‑1 (MQ=255)                                                                                    tgctCGCCATTCCCCATGTGGCTTTTGCCACCAGCGCCCTTCTGCTCTTTGCTGACGGAGGGCTGCTTTATGACTATTTCCCGTATTTCACTCCGCCAATGGACCGATTTGGCATCGGTCTGGGCCTCACCCTTGCAGTGAAAGAAAGCGCATTTCTGCTGTGAATCTTAGCAGCAGTATTGAGCGAAAAATGGCTGTTGCAGCAGGTCATTGTGCTGGATTCGCTGGGCTACAGCCGCTGGCAATGCCTg                                                                                                                                                         >  1:99729/1‑251 (MQ=255)                                                                                     gctCGCCATTCCCCATGTGGCTTTTGCCACCAGCGCCCTTCTGCTCTTTGCTGACGGAGGGCTGTTTTATGACTATTTCCCGTATTTCACTCCGCCAATGGACCGATTTGGCATCGGTCTGGGCCTCACCCTTGCAGTGAAAGAAAGCGCATTTCTGCTGTGAATCTTAGCAGCAGTATTGAGCGAAAAATGGCTGTTGCAGCAGGTCATTGTGCTGGATTCGCTGGGCTACAGCCGCTGGCAATGCCTGa                                                                                                                                                        <  2:329694/251‑1 (MQ=255)                                                                                        cGCCATTCCCCATGTGGCTTTTGCCACCAGCGCCCTTCTGCTCTTTGCTGACGGAGGGCTGCTTTATGACTATTTCCCGTATTTCACTCCGCCAATGGACCGATTTGGCATCGGTCTGGGCCTCGCCCTTGCAGTGAAAGAAAGCGCATTTCTGCTGTGAATCTTAGCAGCAGTATTGAGCGAAAAATGGCTGTTGCAGCAGGTCATTGTGCTGGATTCGCTGGGCTACAGCCGCTGGCAATGCCTGaa                                                                                                                                                       <  1:283586/249‑1 (MQ=255)                                                                                         gCCATTCCCCATGTGGCTTTTGCCACCAGCGCCCTTCTGCTCTTTGCTGACGGAGGGCTGCTTTATGACTATTTCCCGTATTTCACTCCGCCAATGGACCGATTTGGCATCGGTCTGGGCCTCACCCTTGCAGTGAAAGAAAGCGCATTTCTGCTGTGAATCTTAGCAGCAGTATTGAGCGAAAAATGGCTGTTGCAGCAGGTCATTGTGCTGGATTCGCTGGGCTACAGCCGCTGGCAATGCCTGAACTg                                                                                                                                                    >  2:362317/1‑251 (MQ=255)                                                                                                  cATNTGGCTTTTGCCACCAGCGCCCTTCTGCTCTTTGCTGACGGAGGGCTGCTTTATGACTATTTCCCGTATTTCACTCCGCCAATGGACCGATTTGGCATCGGTCTGGGCCTCACCCTTGCAGTGAAAGAAAGCGCATTTCTGCTGTGAATCTTAGCAGCAGTATTGAGCGAAAAATGGCTGTTGCAGCAGGTCATTGTGCTGGATTCGCTGGGCTACAGCCGCTGGCAATGCCTGAACTGGCTGCTGtt                                                                                                                                           <  2:417951/251‑1 (MQ=255)                                                                                                  cATGTGGCTTTTGCCACCAGCGCCCTTCTGCTCTTTGCTGACGGAGGGCTGCTTTATGACTATTTCCCGTATTTCACTCCGCCAATGGACCGATTTGGCATCGGTCTGGGCCTCACCCTTGCAGTGAAAGAAAGCGCATTTCTGCTGTGAATCTTAGCAGCAGTATTGAGCGAAAAATGGCTGTTGCAGCAGGTCATTGTGCTGGATTCGCTGGGCTACAGCCGCTGGCAATGCCTGAACTGGCTGCTGtt                                                                                                                                           <  1:113992/251‑1 (MQ=255)                                                                                                             tGCCACCAGCGCCCTTCTGCTCTTTGCTGACGGAGGGCTGCTTTATGACTATTTCCCGTATTTCACTCCGCCAATGGACCGATTTGGCATCGGTCTGGGCCTCACCCTTGCAGTGAAAGAAAGCGCATTTCTGCTGTGAATCTTAGCAGCAGTATTGAGCGAAAAATGGCTGTTGCAGCAGGTCATTGTGCTGGATTCGCTGGGCTACAGCCGCTGGCAATGCCTGAACTGGCTGCTGTTGCCCTCCGTcg                                                                                                                                >  2:336706/1‑251 (MQ=255)                                                                                                                 accaGCGCCCTTCTGCTCTTTGCTGACGGAGGGCTGCTTTATGACTATTTCCCGTATTTCACTCCGCCAATGGACCGATTTGGCATCGGTCTGGGCCTCACCCTTGCAGTGAAAGAAAGCGCATTTCTGCTGTGAATCTTAGCAGCAGTATTGAGCGAAAAATGGCTGTTGCAGCAGGTCATTGTGCTGGATTCGCTGGGCTACAGCCGCTGGCAATGCCTGAACTGGCTGCTGTTGCCCTCCGTCGCGcc                                                                                                                            >  1:275677/1‑251 (MQ=255)                                                                                                                    aGCGCCCTTCTGCTCTTCGCTGACGGAGTGCTTCTTTATGACTATTTCCCGTATTTCACTCCGCCAATGGACCGATTTGGCATCGGTCTGGGCCTCACCCTTGCAGTGAAAGAAAGCGTATTTCTGCTGTTAATCTTAGCAGCAGTATTGAGCGAAAAATGGCTGTTGCAGCAGGTCATTGTGCTGGATGCGCTGGGCTACAGCCGCTGGCAATGCCTTAACTGGCTGCTGTTGCCCTCCGTCGCGCCTgc                                                                                                                         <  1:159377/251‑1 (MQ=255)                                                                                                                       gcCCTTCTGCTCTTTGCTGACGGAGGGCTGCTTTATGACTATTTCCCGTATTTCACTCCGCCAATGGACCGATTTGGCATCGGTCTGGGCCTCACCCTTGCAGTGAAAGAAAGCGCATTTCTGCTGTGAATCTTAGCAGCAGTATTGAGCGAAAAATGGCTGTTGCAGCAGGTCATTGTGCTGGAt                                                                                                                                                                                       <  2:87247/186‑1 (MQ=255)                                                                                                                       gcCCTTCTGCTCTTTGCTGACGGAGGGCTGCTTTATGACTATTTCCCGTATTTCACTCCGCCAATGGACCGATTTGGCATCGGTCTGGGCCTCACCCTTGCAGTGAAAGAAAGCGCATTTCTGCTGTGAATCTTAGCAGCAGTATTGAGCGAAAAATGGCTGTTGCAGCAGGTCATTGTGCTGGAt                                                                                                                                                                                       >  1:87247/1‑186 (MQ=255)                                                                                                                            tCTGCTCTTTGCTGACGGAGGGCTGCTTTATGACTATTTCCCGTATTTCACTCCGCCAATGGACCGATTTGGCATCGGTCTGGGCCTCACCCTTGCAGTGAAAGAAAGCGCATTTCTGCTGTGAAGCTTAGCAGCAGTATTGAGCGAAAAATGGCTGTTGCAGCAGGTCATTGTGCTGGATTCGCTGGGCTACAGCCGCTGGCAATGCCTGAACTGGCTGCTGTTGCCCTCCGTCGCGCCTGCGCTGGc                                                                                                                   <  2:187538/249‑1 (MQ=255)                                                                                                                                 tctTTGCTGACGGAGGGCTGCTTTATGACTATTTCCCGTATTTCACTCCGCCAATGGACCGATTTGGCATCGGTCTGGGCCTCACCCTTGCAGTGAAAGAAAGCGCATTTCTGCTGTGAATCTTAGCAGCAGTATTGAGCGAAAAATGGCTGTTGCAGCAGGTCATTGTGCTGGATTCGCTGGGCTACAGCCGCTGGCAATGCCTGAACTGGCTGCTGTTGCCCTCCGTCGCGCCTGCGCTGGCAATGGCg                                                                                                            >  2:169724/1‑251 (MQ=255)                                                                                                                                  ctTTGCTGACGGAGGGCTGCTATATGACTATTTCCCGTATTTCACTCCGCCAATGGACCGATTTGGCATCGGTCTGGGCCTCACCCTTGCAGTGCAAGAAAGAGCATTTCTGCTGTGAATCTTAGCAGCAGTATTGAGCGAAAAATGGCTGTTGCAGCAGGTCATTGTGCTGGATTCGCTGGGCTACAGCCGCTGGCAATGCCTGAACTGGCTGCTGTTGCCCTCCGTCGCGCCTGCGCTGGCAATGGCgc                                                                                                           >  2:182059/1‑250 (MQ=255)                                                                                                                                    ttGCTGACGGAGGGCTGCTTTATGACTATTTCCCGTATTTAACTCCGCCAATGGACCGATTTGGCATCGGTCTGGGCCTCACCCTTGCAGTGAAAGAAAGCGCATTTCTGCTGTGAATCTTAGCAGCAGTATTGAGCGAAAAATGGCTGTTGCAGCAGGTCATTGTGCTGGATTCGCTGGGCTACAGCCGCTGGCAATGCCTGAACTGGCTGCTGTTGCCCTCCGTCGCGCCTGCGCTGGCAATGGCGAt                                                                                                          <  2:360784/250‑1 (MQ=255)                                                                                                                                                 gctgctTTATGACTATTTCCCGTATTTCACTCCGCCAATGGACCGATTTGGCATCGGTCTGGGCCTCACCCTTGCAGTGAAAGAAAGCGCATTTCTGCTGTGAATCTTAGCAGCAGTATTGAGCGAAAAATGGCTGTTGCAGCAGGTCATTGTGCTGGATTCGCTGGGCTACAGCCGCTGGCAATGCCTGAACTGGCTGCTGTTGCCCTCCGTCGCGCCTGCGCTGGCAATGGCGATGCTGGCGAttgttg                                                                                            <  2:274422/251‑1 (MQ=255)                                                                                                                                                     ctTTATGACTATTTCCCGTATTTCACTCCGCCAATGGACCGATTTGGCATCGGTCTGGGCCTCACCCTTGCAGTGAAAGAAAGCGCATTTCTGCTGTGAATCTTAGCAGCAGTATTGAGCGAAAAATGGCTGTTGCAGCAGGTCATTGTGCTGGATTCGCTGGGCTACAGCCGCTGGCAATGCCTGAACTGGCTGCTGTTGCCCTCCGTCGCGCCTGCGCTGGCAATGGCGATGCTGGCGATTGTTGCCTg                                                                                        >  2:44895/1‑251 (MQ=255)                                                                                                                                                       ttATGACTATTTCCAGTATTTCACTCCGCCAATGGACCGATTTGGCATCGGTCTGGGCCTCAGCCTTGCAGTGAAAGAAAGCTCATTTCTGCTGTGAATCTTAGCAGTAGTATTGAGCGAAAAACGGCTGTTGCAGCAGGTCATTGTGCTGGATTCGCTGCGCTACAGCCGCTGGCAATGCCTGAACTGGCTGCTGTTGCCCGCCGTCGCGCCTGCGCTGGCAATGGCGATGCTGGCGATTGTTGCCt                                                                                         <  2:158765/248‑1 (MQ=255)                                                                                                                                                         aTGACTATTTCCCGTATTTCACTCCGCCAATGGACCGATTTGGCATCGGTCTGGGCCTCACCCTTGCAGTGAAAGAAAGCGCATTTCTGCTGTGAATCTTAGCAGCAGTATTGAGCGAAAAATGGCTGTTGCAGCAGGTCATTGTGCTGGATTCGCTGGGCTACAGCCGCTGGCAATGCCTGAACTGGCTGCTGTTGCCCTCCGTCGCGCCTGCGCTGGCAATGGCGATGCTGGCGATTGTTGCCTGGTCg                                                                                    <  1:414515/251‑1 (MQ=255)                                                                                                                                                            aCTATTTCCCGTATTTCACTCCGCCAATGGACCGATTTGGCATCGGTCTGGGCCTCACCCTTGCAGTGAAAGAAAGCGCATTTCTGCTGTGAATCTTAGCAGCAGTATTGAGCGAAAAATGGCTGTTGCAGCAGGTCATTGTGCTGGATTCGCTGGGCTACAGCCGCTGGCAATGCCTGAACTGGCTGCTGTTGCCCTCCGTCGCGCCTGCGCTGGCAATGGCGATGCTGGCGATTGTTGCCTGGTCGCTg                                                                                 <  1:157068/251‑1 (MQ=255)                                                                                                                                                               aTTTCCCGTATTTCACTCCGCCAATGGACCGATTTGGCATCGGTCTGGGCCTCACCCTTGCAGTGAAAGAAAGCGCATTTCTGCTGTGAATCTTAGCAGCAGTATTGAGCGAAAAATGGCTGTTGCAGCAGGTCATTGTGCTGGATTCGCTGGGCTACAGCCGCTGGCAATGCCTGAACTGGCTGCTGTTGCCCTCCGTCGCGCCTGCGCTGGCAATGGCGATGCTGGCGATTGTTGCCTGGTCGCTgtcg                                                                              <  1:111779/251‑1 (MQ=255)                                                                                                                                                                   cccGTATTTCACTCCGCCAATGGACCGATTTGGCATCGGTCTGGGCCTCACCCTTGCAGTGAAAGAAAGCGCATGTCTGCTGTGAATCTTAGCAGCAGTATTGAGCGAAAAATGGCTGTTGCAGCAGGTCATTGTGCTGGATTCGCTGGGCTACAGCCGCTGGCAATGCCTGAACTGGCTGCTGTTGCCCTCCGTCGCGCCTGCGCTGGCAATGGCGATGCTGGCGATTGTTGCCTGGTCGCTgtcggtc                                                                           <  1:331815/250‑1 (MQ=255)                                                                                                                                                                         tttCACTCCGCCAATGGACCGATTTGGCATCGGTCTGGGCCTCACCCTTGCAGTGAAAGAAAGCGCATTTCTGCTGTGAATCTTAGCAGCAGTATTGAGCGAAAAATGGCTGTTGCAGCAGGTCATTGTGCTGGATTCGCTGGGCTACAGCCGCTGGCAATGCCTGAACTGGCTGCTGTTGCCCTCCGTCGCGCCTGCGCTGGCAATGGCGATGCTGGCGATTGTTGCCTGGTCGCTGTCGGTCGGGGAtg                                                                    >  2:331379/1‑251 (MQ=255)                                                                                                                                                                             ccTCCGCCAATGGACCGATTTGGCATCGGTCTGGGCCTCACCCTTGCAGTGAAAGAAAGCGCATTTCTGCTGTGAATCTTAGCAGCAGTATTGAGCGAAAAATGGCTGTTGCAGCAGGTCATTGTGCTGGATTCGCTGGGCTACAGCCGCTGGCAATGCCTGAACTGGCTGCTGTTGCCCTCCGTCGCGCCTGCGCTGGCAATGGCGATGCTGGCGATTGTTGCCTGGTCGCTGTCGGTCGTGGATGTGGc                                                                <  1:285115/250‑1 (MQ=255)                                                                                                                                                                             aCTCCGCCAATGGACCGATTTGGCATCGGTCTGGGCCTCACCCTTGCAGTGAAAGAAAGCGCATTTCTGCTGTGAATCTTAGCAGCAGTATTGAGCGAAAAATGGCTGTTACAGCAGGTCATTGTGCTGGATTCGCTGGGCTACAGCCGCTGGCAATGCCTGAACTGGCTGCTGTTGCCCTCCGTCGCGCCTGCGCTGGCAATGGCGATGCTGGCGATTGGTGCCTGGTCGCTGTCGGGCGTGGGTGTGGc                                                                >  2:352511/1‑251 (MQ=255)                                                                                                                                                                                        ggACCGATTTGGCATCGGTCTGGGCCTCACCCTTGCAGTGAAAGAAAGCGCATTTCTGCTGTGAATCTTAGCAGCAGTATTGAGCGAAAAATGGCTGTTGCAGCAGGTCATTGTGCTGGATTCGCTGGGCTACAGCCGCTGGCAATGCCTGAACTGGCTGCTGTTGCCCTCCGTCGCGCCTGCGCTGGCAATGGCGATGCTGGCGATTGTTGCCTGGTCGCTGTCGGTCGTGGATGTGGCAATTATTCTCg                                                     >  2:46466/1‑251 (MQ=255)                                                                                                                                                                                             gATTTGGCATCGGTCTGTGCCTCACCCTTGCAGTGAAAGAAAGCGCATTTCTGCTGTGAATCTTAGCAGCAGTATTGAGCGAAAAATGGCTGTTGCAGCCGGTCATTGTGCTGGATTCGCTGGGCTACATCCGCTGGCAATGCCTGAACTGGCTGCTGTTGCCCTCCGTCGCGCCTGCGCTGGCAATGGCGATGCTGGCGATTGTTGCCTGGTCGCTGTCGGTCGTGGATGTGGCAATTATTCTCGGGCCa                                                <  1:223510/251‑1 (MQ=255)                                                                                                                                                                                             gATTTGGCATCGGTCTGGGCCTCACCCTTGCAGTGAAAGAAAGCGCATTTCTGCTGTGAATCTTAGCAGCAGTATTGAGCGAAAAATGGCTGTTGCAGCAGGTCATTGTGCTGGATTCGCTGGGCTACAGCCGCTGGCAATGCCTGAACTGGCTGCTGTTGCCCTCCGTCGCGCCTGCGCTGGCAATGGCGATGCTGGCGATTGTTGCCTGGTCGCTGTCGGTCGTGGATGTGGCAATTATTCTCGGGCCa                                                <  2:450475/251‑1 (MQ=255)                                                                                                                                                                                                  ggCATCGGTCTGGGCCTCACCCTTGCAGTGAAAGAAAGCGCATTTCTGCTGTGAATCTTAGCAGCAGTATTGAGCGAAAAATGGCTGTTGCAGCAGGTCATTGTGCTGGATTCGCTGGGCTACAGCCGCTGGCAATGCCTGAACTGGCTGCTGTTGCCCTCCGTCGCGCCTGCGCTGGCAATGGCGATGCTGGCGGTTGTTGCCTGGTCGCTGTCGGGAGTGGATGTGGCAATTATTCTCGGGCCAGGTa                                            >  2:78967/1‑250 (MQ=255)                                                                                                                                                                                                             gggCCTCACCCTTGCAGTGAAAGAAAGCGCATTTCTGCTGTGAATCTTAGCAGCAGTATTGAGCGAAAAATGGCTGTTGCAGCAGGTCATTGTGCTGGATTCGCTGGGCTACAGCCGCTGGCAATGCCTGAACTGGCTGCTGTTGCCCTCCGTCGCGCCTGCGCTGGCAATGGCGATGCTGGCGATTGTTGCCTGGTCGCTGTCGGTCGTGGATGTGGCAATTATTCTCGGGCCAGGTAATCCCCCGAc                                  <  2:364569/249‑1 (MQ=255)                                                                                                                                                                                                               gCCTCACCCTTGCAGTGAAAGAAAGCGCATTTCTGCTGTGAATCTTAGCAGCAGTATTGAGCGAAAAATGGCTGTTGCAGCAGGTCATTGTGCTGGATTCGCTGGGCTACAGCCGCTGGCAATGCCTGAACTGGCTGCTGTTGCCCTCCGTCGCGCCTGCGCTGGCAATGGCGATGCTGGCGATTGTTGCCTGGTCGCTGTCGGTCGTGGATGTGGCAATTATTCTCGGGCCAGGTAATCCCCCGACGCTg                              <  1:331379/251‑1 (MQ=255)                                                                                                                                                                                                               gCCTCACCCTTGCAGTGAAAGAAAGCGCATTTCTGCTGTGAATCTTAGCAGCAGTATTGAGCGAAAAATGGCTGTTGCAGCAGGTCATTGTGCTGGATTCGCTGGGCTACAGCCGCTGGCAATGCCTGAACTGGCTGCTGTTGCCCTCCGTCGCGCCTGCGCTGGCAATGGCGATGCTGGCGATTGTTGCCTGGTCGCTGTCGGTCGTGGATGTGGCAATTATTCTCGGGCCAGGTAATCCCCCGACGCTg                              <  2:153555/251‑1 (MQ=255)                                                                                                                                                                                                                 cTCACCCTTGCAGTGAAAGAAAGCGCATTTCTGCTGTGAATCTTAGCAGCAGTATTGAGCGAAAAATGGCTGTTGCAGCAGGTCATTGTGCTGGATTCGCTGGGCTACAGCCGCTGGCAATGCCTGAACTGGCTGCTGTTGCCCTCCGTCGCGCCTGCGCTGGCAATGGCGATGCTGGCGATTGTTGCCTGGTCGCTGTCGGTCGTGGATGTGGCAATTATTCTCGGGCCAGGTAATCCCCCGACGCTGGc                            >  1:433506/1‑251 (MQ=255)                                                                                                                                                                                                                   cACCCTTGCAGTGAAAGAAAGCGCATTTCTGCTGTGAATCTTAGCAGCAGTATTGAGCGAAAAATGGCTGTTGCAGCAGGTCATTGTGCTGGATTCGCTGGGCTACAGCCGCTGGCAATGCCTGAACTGGCTGCTGTTGCCCTCCGTCGCGCCTGCGCTGGCAATGGCGATGCTGGCGATTGTTGCCTGGTCGCTGTCGGTCGTGGATGTGGCAATTATTCTCGGGCCAGGTAATCCCCCGACGCTGGc                            <  1:2651/249‑1 (MQ=255)                                                                                                                                                                                                                   cACCCTTGCAGTGAAAGAAAGCGCATTTCTGCTGTGAATCTTAGCAGCAGTATTGAGCAAAAAATTGCTGTTGCAGCTGGTCATTGTGCTGGATTCGCTGGGCTTCAGCCGCTGGCAATGCCTGAACTGGCTGCTGTTGCCCTCCGTCGCGCCTGCGCGGGCAATGGCGATGCTTGCGATTGTTGCCTGGTCGCTGTCGGTCGTGTATGTGGCAATTATTCTCGGGCCAGGTAATCCCCCGACTCTGGCgg                          <  2:165047/251‑1 (MQ=255)                                                                                                                                                                                                                          gCAGTGAAAGAAAGCGCATTTCTGCTGTGAATCTTAGCAGCAGTATTGAGCGAAAAATGGCTGTTGCAGCAGGTCATTGTGCTGGATTCGCTGGGGTACAGCCGCTGGCAATGCCTGAACTGGCTGCTGTTGCCCTCCGTCGCGCCTGCGCTGGCAGTGGCGATGCTGGCGATTGTTGCCTGGTCGCTGTCGGTCGTGGATGTGGCAATTATTCTCGGGCAAGGTAATCCCCCGCCGCTGGCGGttatta                    >  2:299597/1‑250 (MQ=255)                                                                                                                                                                                                                          gCAGTGAAAGAAAGCGCATTTCTGCTGTGAATCTTAGCAGCAGTATTGAGCGAAAAATGGCTGTTGCAGCAGGTCATTGTGCTGGATTCGCTGGGCTACAGCCGCTGGCAATGCCTGAACTGGCTGCTGTTGCCCTCCGTCGCGCCTGCGCTGGCAATGGCGATGCTGGCGATTGTTGCCTGGTCGCTGTCGGTCGTGGATGTGGCAATTATTCTCGGGCCAGGTAATCCCCCGACGCTGGCGGTAAtt                     >  1:430270/1‑249 (MQ=255)                                                                                                                                                                                                                           cAGTGAAAGAAAGCGCATTTCTGCTGTGAATCTTAGCAGCAGTATTGAGCGAAAAATGGCTGTTGCAGCAGGTCATTGTGCTGGATTCGCTGGGCTACAGCCGCTGGCAATGCCTGAACTGGCTGCTGTTGCCCTCCGTCGCGCCTGCGCTGGCAATGGCGATGCTGGCGATTGTTGCCTGGTCGCTGTCGGTCGTGGATGTGGCAATTATTCTCGGGCCAGGTAATCCCCCGACGCTGGCGGTAATTAGc                  <  1:367739/251‑1 (MQ=255)                                                                                                                                                                                                                            aGTGAAAGAAAGCGCATTTCTGCTGTGAATCTTAGCAGCAGTATTGAGCGAAAAATGGCTGTTGCAGCAGGTCATTGTGCTGGATTCGCTGGGCTACAGCCGCTGGCAATGCCTGAACTGGCTGCTGTCGCCCTCCGTCGCGCCTGCGCTGGCAATGGCGATGCTGGCGATTGTTGCCTGGTCGCTGTCGGTCGTGGATGTGGCAATTATTCTCGGGCCAGGTAATCCCCCGACGCTGGCGGTAATTAGCt                 >  1:173999/1‑251 (MQ=255)                                                                                                                                                                                                                                  agaaagCGCATTTCTGCTGTGAATCTTAGCAGCAGTATTGAGCGAAAAATGGCTGTTGCAGCAGGTCATTGTGCTGGATTCGCTGGGCTACAGCCGCTGGCAATGCCTGAACTGGCTGCTGTTGCCCTCCGTCGCGCCTGCGCTGGCAATGGCGATGCTGGCGATTGTTGCCTGGTCGCTGTCGGTCGTGGATGTGGCAATTATTCTCGGGCCAGGTAATCCCCCGACGCTGGCGGTAATTAGCTGGCAGt           <  1:370276/251‑1 (MQ=255)                                                                                                                                                                                                                                   gaaagCGCATTGCTGCTGTGAAGCTTAGCAGCAGTATTGAGCGAAAAATGGCTGTTGCAGCAGGTCATTGTGCGGGATGCGCGGGGCTACAGCCGCTGGCAATGCCTGAACGGGCTGCTGTTGCCCTCCGTCGCGACTGCGCTGGCAAGGGCGATGCTGGCGATTGTTGCCTGGTCGCTGTCGGTCGTGGATGGGGCAATTATTCTCGGGCCAGGTAATCCCCCAACGCGGGCGGTAATTAGCTGGCAGTg          <  2:190651/251‑1 (MQ=255)                                                                                                                                                                                                                                    aaagCGCATTTCTGCTGTGAATCTTAGCAGCAGTATTGAGCGAAAAATGGCTGTTGCCGCAGGTCATTGTGCTGGATTCGCTGGGCTCCAGCCGCTGGCAATGCCTGAACTGGCTGCTTTTGCCCTCCGTCGCGCCTGCGCTGGCAATGGCGTTGCTGGCGCTTGTTGCCTGGATGCTGTCGGTCGTGGATGTGGCCATTATTCTCGGGCCAGGTAATCCCCCGACGCTGGCGGTAATTAGCTGGCAGTgg         >  2:436955/1‑251 (MQ=255)                                                                                                                                                                                                                                     aagCGCAATTCTGCTGTGAATCTTAGCAGCAGTATTGAGCGAAAAATGGCTGTTGCAGCAGGTCATTGTGCTGGATTCGCTGGGCTACAGCCGCTGGCAATGCCTGAACTGGCTGCTGTTGCCCTCCGTCGCGCCTGCGCTGGCAATGGCGATGCTGGCGATTGTTGCCTGGTCGCTGTCGGTCGTGGATGTGGCAATTATTCTCGGGCCAGGTAATCCCCCGACGCTGGCGGTAATTAGCTGGCAGTGGt        >  1:402888/1‑251 (MQ=255)                                                                                                                                                                                                                                         gcATTTCTGCTGTGAATCTTAGCAGCAGTATTGAGCGAAAAATGGCTGTTGCAGCAGGTCAGTGTGCTGGATTCGCTGGGCTACAGCCGCTGGCAATGCCTGAACTGGCTGCTGTTGCCCTCCGTCGCGCCTGCGCTGGCAATGGCGATGCTGGCGATTGTTGCCTGGTCGCTGTCGGTCGTGGATGTGGCAATTATTCTCGGGCCAGGTAATCCCCCGACGCTGGCGGTAATTAGCTGGCAGTGGTTAAc    <  1:180172/251‑1 (MQ=255)                                                                                                                                                                                                                                          cATNTCTGCTGTGAATCTTAGCAGCAGTATTGGGCGAAAAATGGCTGTGCCAGCAGGTCATTGTGCTGGATTCGCTGGGCTACAGCCCGTGGCAATGCCTGAACGGGCTGCGGTTGCCCTCCGTCGCGCCGGCGCTGGCAATGGCTATGCTGGCGATTGTTGCCTGGTCGCTGGCAGTCTTGGATGTGGCAATGATTCTCGGGTCGGATAACCCCCCGACGCTTTCGTTAATTAGCTGGCAGTGGTTAAcc   <  2:408128/251‑1 (MQ=255)                                                                                                                                                                                                                                           aTTTCTGCTGTGAATCTTAGCAGCAGTATTGAGCGAAAAATGGCTGTTGCAGCAGGTCATTGTGCTGGATTGGCTGGGCTACAGCCGCCGGCAATGCCTGAACTGGCTGCTGTTGCCCTCCGTCGCGCCTGCCCTGGCAATGGCGATGCTTGCGATTGTTACCTGGTCGCTGTCGGCCGTGGATGTGGCCATTATTCTCGGGCCCGGTAATCCCCCCACGCTGGCGGTAATTATCTGGCCGTGGTTGAccc  >  2:201853/1‑251 (MQ=255)                                                                                                                                                                                                                                           aTTTCTGCTGTGAATCTTAGCAGCAGTATTGAGCGAAAAATGGCTGTTGCAGCAGGTCATTGTGCTGGATTCGCTGGGCTACAGCCGCTGGCAATGCCTGAACTGGCTGCTGTTGCCCTCCGTCGCGCCTGCGCTGGCAATGGCGATGCTGGCGATTGTTGCCTAGTCGCTGTCGGTCGTGGATGTGGCAATTATTCTCGGGCCAGGTAATCCCCCGACGCTGGCGGTAATTAGCTGGCAGTGGTTAAccc  >  1:432314/1‑251 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                               GGCATTGTTGATTGCCCTGCTGGTGATTGTGGCGCTGTGGCCTGGGCCGAAATGGCAGCGTATGTGCGCCCGTCTGCCGTGGCTGCTCGCCATTCCCCATGTGGCTTTTGCCACCAGCGCCCTTCTGCTCTTTGCTGACGGAGGGCTGCTTTATGACTATTTCCCGTATTTCACTCCGCCAATGGACCGATTTGGCATCGGTCTGGGCCTCACCCTTGCAGTGAAAGAAAGCGCATTTCTGCTGTGGATCTTAGCAGCAGTATTGAGCGAAAAATGGCTGTTGCAGCAGGTCATTGTGCTGGATTCGCTGGGCTACAGCCGCTGGCAATGCCTGAACTGGCTGCTGTTGCCCTCCGTCGCGCCTGCGCTGGCAATGGCGATGCTGGCGATTGTTGCCTGGTCGCTGTCGGTCGTGGATGTGGCAATTATTCTCGGGCCAGGTAATCCCCCGACGCTGGCGGTAATTAGCTGGCAGTGGTTAACCC  >  NC_000913/1837412‑1837896

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑