Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,242,121	A→G	S283P (TCG→CCG)	yeiB ←	DUF418 domain‑containing protein YeiB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,242,121	0	A	G	98.0%	146.7 / NA	49	S283P (TCG→CCG)	yeiB	DUF418 domain‑containing protein YeiB
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (25/23);  minor base C (1/0);  total (26/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCCTGGCGGAAGTAACGCAGCCAGATAACAGAGAAGAGGATATTCGCCAGCCATACCGGAATAACAAACGCCAGCAGCTCCAGGCGGTCAAAATGCATAAACAAACCGAGGTGGTAAAAAAGCGTGGTACAAATCAGCGTTTGCAATAGATAGTTGGTCAGCGCCATCCGTCCGACGCAGGCGATCGCAAGCACCAGCTTAAAGCGGCTCAATTGCGGCCAGAAGCCATAAAACAGCGACGCATAGCCAATCGCCTGAAACGGCGCACTCAGTTCCCGCGGCATTTGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCGATGGCAGGAAGGTTAATGGTCACCCCAATCGCCACCAGCACAAAACCAGTACGACGATAGTGACGTAAGCTGAACTGCCCTTTCAGCCAGCCGCTGCGCATCAGCGCCGCACCAATGAGCATCATCCCTGCCAGTTGCCAGCCAT  >  NC_000913/2241883‑2242366                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                      cccTGGCGGAAGTAACGCAGCCAGATAACAGAGAAGAGGATATTCGCCAGCCATACCGGAATAACAAACGCCAGCAGCTCCAGGCGGTCAAAATGCATAAACAAACCGAGGTGGTAAAAAAGCGTGGTACAAATCAGCGTTTGCAATAGATAGTTGGTCAGCGCCATCCGTCCGACGCAGGCGATCGCAAGCACCAGCTTAAAGCGGCTCAATTGCGGCCAGAAGCCATAAAACAGCGGCGCATAGCCAAt                                                                                                                                                                                                                                           >  2:264786/1‑251 (MQ=255) cccNGGCGGAAGTAACGCAGCAAGATAACAGAGAAGATGATATTCCCCAGCCATACCGGAATAACAAACGCCAGCAGCTCCAGGCGGTCAAAATGCATAAACAAACCGAGGTGGTAAAAAAGCGTGGTACAAATCAGCGTGTGCAATAGATATTTGGTCAGCGCCATCCGTCCGACGCAGGCGATCGCAAGCACCAGCTTAAAGCGGCGCAATGGCGGCCAGAAGCCATAAAACAGCGGCGCATAGCCAAt                                                                                                                                                                                                                                           <  2:419963/251‑1 (MQ=255)     ggCGGAAGTAACGCAGCCAGATAACAGAGAAGAGGATATTCGCCAGCCATACCGGAATAACAAACGCCAGCAGCTCCAGGCGGTCAAAATGCATAAACAAACCGAGGTGGTAAAAAAGCGTGGTACAAATCAGCGTTTGCAATAGATAGTTGGTCAGCGCCATCCGTCCGACGCAGGCGATCGCAAGCACCAGCTTAAAGCGGCTCAATTGCGGCCAGAAGCCATAAAACAGCGGCGCATAGCCAATCGcc                                                                                                                                                                                                                                       >  2:99092/1‑251 (MQ=255)                    gccgaatacaggatattagGATATGCGCCAGCCATACCGGAATAACAAACGCCTGCAGCTCCAGGCGGTCAAAATGCATAATCAAACCGCGGTGGTAAAAAAGCGTGTGACAGTTCAGCGTTTGCAATAGATAGTTGGTCAGCGCCATCCGTCCGACGCAGGCGATCGCAAGCACCAGCTTAAAGCGGCTCAATTGCGGCCAGAAGCCTTAATACAGCGGCGCATAGCCAATCGCCTGAAACGGCGCACTc                                                                                                                                                                                                                        <  2:293006/234‑1 (MQ=255)                                   agagGATATTCGCCAGCCATACCGGAATAACAAACGCCAGCAGCTCCAGGCGGTCAAAATGCATAAACAAACCGAGGGGGTAAAAAAGCGTGGCACAAATCAGCGTTTGCAATAGATAGTTGGTCAGCGCCATCCGTCCGACGCAGGCGATCGCCAGCACCAGCTTAAAGCGGCTCAATTGCGGCCAGAAGCCATAAAACAGCGCCGCATAGCCAATCGCCTGAAACGGCGCACTCAGTTCCCGCGGCAtt                                                                                                                                                                                                         >  2:331642/1‑251 (MQ=255)                                     agGATATTCGCCAGCCATACCGGAATAACAAACGCCAGCAGCTCCAGGCGGTCAAAATGCATAAACAAACCGAGGTGGTAAAAAAGCGTGGTACAAATCAGCGTTTGCAATAGATAGTTGGTCAGCGCCATCCGTCCGACGCAGGCGATCGCAAGCACCAGCTTAAAGCGGCTCAATTGCGGCCAGAAGCCATAAAACAGCGGCGCATAGCCAATCGCCTGAAACGGCGCACTCAGTTCCCGCGGCATTTg                                                                                                                                                                                                       >  1:450715/1‑251 (MQ=255)                                        atatTCGCCAGCCATACCGGCATAACAAACGCCAGCAGCTCCAGGCGGTCAAAATGCATAAACAAACCGAGGTGGTAAAAAAGCGTGGTACAAATCAGCGTTTGCAATAGATAGTTGGTCAGCGCCATCCGTCCGACGCAGGCGATCGCAAGCACCAGCTTAAAGCGGCTCAATTGCGGCCAGAAGCCATAAAACAGCGGCGCATAGCCAATCGCCTGAAACGGCGCACTCAGTTCCCGCGGCATTTGa                                                                                                                                                                                                      >  1:315121/1‑249 (MQ=255)                                             cGCCAGCCATACCGGAATAACAAACGCCAGCAGCTCCAGGCGGTCAAAATGCATAAACAAACCGAGGTGGTAAAAAAGCGTGGTACAAATCAGCGTTTGCAATAGATAGTTGGTCAGCGCCATCCGTCCGACGCAGGCGATCGCAAGCACCAGCTTAAAACGGCTCAATTGCGGCCAGAAGCCATAAAACAGCGGCGCATAGCCAATCGCCTGAAACGGCGCACTCAGTTCCCGCGGCATTTGAAGTa                                                                                                                                                                                                  >  1:427662/1‑248 (MQ=255)                                                  gccaTACCGGAATAACAAACGCCAGCAGCTCCAGGCGGTCAAAATGCATAAACAAACCGAGGTGGTAAAAAAGCGTGGTACAAATCAGCGTTTGCAATAGATAGTTGGTCAGCGCCATCCGTCCGACGCAGGCGATCGCAAGCACCAGCTTAAAGCGGCTCAATTGCGGCCAGAAGCCATAAAACAGCGGCGCATAGCCAATCGCCTGAAACGGCGCACTCAGTTCCCGCGGCATTTGAAGTAAGAAGGca                                                                                                                                                                                          <  2:368266/251‑1 (MQ=255)                                                   ccaTACCGGAATAACAAACGCCAGCAGCTCCAGGCGGTAAAAATGCATAAACAAACCGAGGTGGTAAAAAAGCGTGGTACAAATCAGCGTTTGCAATAGATAGTTGGTCAGCGCCATCCGTCCGACGCAGGCGATCGCAAGCACCAGCTTAAAGCGGCTCAATTGCGGCCAGAAGCCATAAAACAGCGGCGCATAGCCAATCGCCTGAAACGGCGCACTCAGTTCCCGCGGCATTTGAAGTAAGAAGGcac                                                                                                                                                                                         <  1:92230/251‑1 (MQ=255)                                                        ccGGAATAACAAACGCCAGCAGCTCCAGGCGGTCAAAATGCATAAACAAACCGAGGTGGTAAAAAAGCGTGGTACAAATCAGCGTTTGCAATAGATAGTTGGTCAGCGCCATCCGTCCGACGCAGGCGATCGCAAGCACCAGCTTAAAGCGGCTCAATTGCGGCCAGAAGCCATAAAACAGCGGCGCATAGCCAATCGCCTGAAACGGCGCACTCAGTTCCCGCGGCATTTGAAGTAAGAAGGCACACCAg                                                                                                                                                                                    <  1:237161/251‑1 (MQ=255)                                                                   aaCGCCAGCAGCTCCAGGCGGTCAAAATGCATAAACAACACGAGGGGGTAAAAAAGCGTGTTACAAAACAGCTTTTGCAATAGATAGTTGGTCAGCGCCATCCGTCCGACGCAGGCGATCGCAAGCACCAGCGTAAAGCGGCTCAATTGCGGCCAGAAGCCATAAAACAGCGGCGCATAGCCAATCGACTGAAACGGCGCACTGAGTTCCCCCGGCATTTGAAGTAAGTAGGCTCACCAGCGAGACGCCCa                                                                                                                                                                         <  2:293083/251‑1 (MQ=255)                                                                        cagcagCTCCAGGCGGTCAAAATGCATAAACAAACCGAGGTGGTAAAAAAGCGTGGTACAAATCAGCGTTTGCAATAGATAGTTGGTCAGCGCCATCCGTCCGACGCAGGCGATCGCAAGCACCAGCTTAAAGCGGCTCAATTGCGGCCAGAAGCCATAAAACAGCGGCGCATAGCCAATCGCCTGAAACGGCGCACTCAGTTCCCGCGGCATTTGAAGTAAGAAGGCACACCAGCGATACGCCCAGTcc                                                                                                                                                                     >  1:349630/1‑250 (MQ=255)                                                                         agcagaaccAGGCGGTCAAAATGCATAAACAAACCGAGGTGGTAACAAAGCGTGGGACAAATCAGCGGTTGCAATAGATAGATGGTCAGCGCCATCAGTCCGAAGAAGGCGATCGCAAGCACCAGGTTAAAGCGGCTCAATTGCGGGCAGAAGCCATAAAAAAGCGGCGCAGAGCCAATCGCGTGAAAAGGCGCACTGAGTTCCCGCGGCATTTGAGGTAAGAAGGCACACCAGCGATACGCGCAGTcc                                                                                                                                                                     >  2:192951/8‑249 (MQ=255)                                                                                     gggccAAAATGCAGAAAAAAGCCGAGGTGGGAAAAAAGCGTGGGACAAATCGGCGTTTGCAAGAGATAGTTGGTCAGCGCCAGCCGTCCGATGCAGGCGAGCGCAAGCACCGGCTTAAATCGGCTGAATTTCGGCCAGAACCCATTAGACATCTGTGCATAGCCAATCGCCTGAAACGGCGCACTCAGTGCCCGCCGCATTTGAAGTAAGAACGCCCACCCGCGGTACGCTCAGTCCAGCTGCCATTGCAg                                                                                                                                                       <  2:294306/247‑1 (MQ=255)                                                                                      ggTCAAAATGCATAAACAAACCGAGGTGGTAAAAAAGCGTGGTACAAATCAGCGTTTGCAATAGATAGTTGGTCAGCGCCATCCGTCCGACGCAGGCGATCGCAAGCACCAGCTTAAAGCGGCTCAATTGCGGCCAGAAGCCATAAAACAGCGGCGCATAGCCAATCGCCTGAAACGGCGCACTCAGTTCCCGCGGCATTTGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAgg                                                                                                                                                      >  2:170333/1‑251 (MQ=255)                                                                                      ggTCAAAATGCATAAACAAACAGAGGTAGTAACAAAGCGTGGTACAAATCAGAGTTTGAAATAGATAGTTGGTCAGAGCCATCCGTCCGACGCAGGCGATCGCAAGCACCAGCTTAAAGCGGCTCAATTGCCGCCAGAAGCCATAAAACAGAGGCGCCTAGCCAATCGCCTGACACGGCCTACTCCGAGCCCGCGGCATTTGAAGTAAGGAAGCACAACAGCGATGCGCCCACGCCAGCCGCTATTGCa                                                                                                                                                        >  2:316115/1‑249 (MQ=255)                                                                                       gTCAAAATGCATAAACAAACCGAGGTGGTAAAAAAGCGTGGTACAAATCAGCGTTTGCAATAGATAGTTGGTCAGCGCCATCCGTCCGACGCAGGCGATCGCAAGCACCAGCTTAAAGCGGCTCAATTGCGGCCAGAAGCCATAAAACAGCGGCGCATAGCCAATCGCCTGAAACGGCGCACTCAGTTCCCGCGGCATTTGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAggg                                                                                                                                                     <  1:170333/251‑1 (MQ=255)                                                                                       gTCAAAATGCATAAACAAACCGAGGTGGTAAAAAAGCGTGGTACAAATCAGCGTTTGCAATAGATAGTTGGTCAGCGCCATCCGTCCGACGCAGGCGATCGCAAGCACCAGCTTAAAGCGGCTCAATTGCGGCCAGAAGCCATAAAACAGCGGCGCATAGCCAATCGCCTGAAACGGCGCACTCAGTTCCCGCGGCATTTGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAggg                                                                                                                                                     <  1:418486/251‑1 (MQ=255)                                                                                         cAAAATGCATAAACAAACCGAGGTGGTAAAAAAGCGTGGTACAAATCAGCGTTTGCAATAGATAGTTGGTCAGCGCCATCCGTCCGACGCAGGCGATCGCAAGCACCAGCTTAAAGCGGCTCAATTGCGGCCAGAAGCCATAAAACAGCGGCGCATAGCCAATCGCCTGAAACGGCGCACTCAGTTCCCGCGGCATTTg                                                                                                                                                                                                       <  2:304697/199‑1 (MQ=255)                                                                                         cAAAATGCATAAACAAACCGAGGTGGTAAAAAAGCGTGGTACAAATCAGCGTTTGCAATAGATAGTTGGTCAGCGCCATCCGTCCGACGCAGGCGATCGCAAGCACCAGCTTAAAGCGGCTCAATTGCGGCCAGAAGCCATAAAACAGCGGCGCATAGCCAATCGCCTGAAACGGCGCACTCAGTTCCCGCGGCATTTg                                                                                                                                                                                                       >  1:304697/1‑199 (MQ=255)                                                                                         aaaaaTGAATAAACAAACCGAGGTGGTAAAAAAGCGTGGTACAAATCAGCGTTTGCAATAGATAGTTGGTCAGCGCCATCCGTCCGACGCAGGCGATGGCAAGCACCAGCTTAAAGCGGCTCAATTGCGGCCAGAAGCCATAAAACAGCGGCGCATAGCCAATCGCCTGAAACGGCGCACTCAGTTCCCGCGGCATTTGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCg                                                                                                                                                   <  2:107412/250‑1 (MQ=255)                                                                                           aaaTGCATAAACAAACCGAGGTGGTAAAAAAGCGTGGTACAAATCAGCGTTTGCAATAGATAGTTGGTCAGCGCCATCCGTCCGACGCAGGCGATCGCAAGCACCAGCTTAAAGCGGCTCAATTGCGGCCAGAAGCCA‑AAAACAGCGGCGCATAGCCAATCGCCTGAAACGGCGCACTCAGTTCCCGCGGCATTTGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCGATg                                                                                                                                                <  2:237873/251‑1 (MQ=255)                                                                                                cATAAACAAACCGAGGTGGTAAAAAAGCGTGGTACAAATCAGCGTTTGCAATAGATAGTTGGTCAGCGCCATCCGTCCGACGCAGGCGATCGCAAGCACCAGCTTAAAGCGGCTCAATTGCGGCCAGAAGCCATAAAACAGCGGCGCATAGCCAATCGCCTGAAACGGCGCACTCAGTTCCCGCGGCATTTGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCGATGGCAg                                                                                                                                            <  1:330813/251‑1 (MQ=255)                                                                                                       aaacCGAGGTGGTAAAAAAGCGTGGTACAAATCAGCGTTTGCAATAGATAGTTGGTCAGCGCCATCCGTCCGACGCAGGCGATCGCAAGCACCAGCTTAAAGCGGCTCAATTGCGGCCAGAAGCCATAAAACAGCGGCGCATAGCCAATCGCCTGAAACGGCGCACTCAGTTCCCGCGGCATTTGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCGATGGCAGGAAGGtt                                                                                                                                     <  2:107576/251‑1 (MQ=255)                                                                                                       aaacCGAGGTGGTAAAAAAGCGTGGTACAAATCAGCGTTTGCAATAGATAGTTGGTCAGCGCCATCCGTCCGACGCAGGCGATCGCAAGCACCAGCTTAAAGCGGCTCAATTGCGGCCAGAAGCCATAAAACAGCGGCGCATAGCCAATCGCCTGAAACGGCGCACTCAGTTCCCGCGGCATTTGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCGATGGCAGGAAGGtt                                                                                                                                     >  2:140906/1‑251 (MQ=255)                                                                                                           cGAGGTGGTAAAAAAGCGTGGTACAAATCAGCGTTTGCAATAGATAGTTGGTCAGCGCCATCCGTCCGACGCAGGCTATCGCAAGCACCAGCTTAAAGCGGCTCAATTGCGGCCAGAATCCATAAAACAGCGGCGCATAGCCAATCGCCTGAAACGGCGCACTCAGTTCCCGCGGCATTTGAAGTAAGAATGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCGATGGCAGGAAGGTTAATg                                                                                                                                 <  2:201517/251‑1 (MQ=255)                                                                                                            gAGGTGGTAAAAAAGAGTGGAACAAATCAGCGTTTGCAATAGATAGTTGGTCAGCGCAATCCGTCCGACGCAGGCGATCGAAAGCACCAGCTTAAAGCGGCTCAATTGCGGCCAGAAGCCATAAAACAGCGGCGCATAGCCAATCGCCTGAAACGGCGCACTCAGTTCCCGCGGCATTTGAAGTAAGAAGGCAAACCAGCGAGACGCCCAGTCCAGCTGCCAGTGCAGCGCGAGGGCAGGAAGGTTAATgg                                                                                                                                >  2:250997/1‑251 (MQ=255)                                                                                                                       aaaGCGTGGTACAAATCAGCGTTTGCAATAGATAGTTGGTCAGCGCCATCCGTCCGACACAGGCGATCGCAAGCACCAGCTTAAAGCGGCTCCATTGCGGCCAGAAGCAATAAAACAGCGGCGCATAGCCAATCGCCTAACACGGCGCACTCAGTTCCCGCGGCCTTTGAAGTAAGAAGGCACACCAGCGAAACGCCCAATCCAGCTGCCATTGCCGGGCGATGGCATGAATGGTAATGGTCACCCCAATc                                                                                                                     >  1:262042/1‑251 (MQ=255)                                                                                                                                   aaaTCAGCGTTTGCAATAGATAGTTGGTCAGCGCCATCCGTCCGACGCAGGCGATCGCAAGCACCAGCTTAAAGCGGCTCAATTGCGGCCAGAAGCCATAAAACAGCGGCGCATAGCCAATCGCCTGAAACGGCGCACTCAGTTCCCGCGGCATTTGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCGATGGCAGGAAGGTTAATGGTCACCCCAATCGCCACCAGcac                                                                                                          >  2:194167/1‑250 (MQ=255)                                                                                                                                                 aaTAGATAGTTGGTCAGCGCCATCCGTCCGACGCAGGCGATCGCAAGCACCAGCTTAAAGCGGCTCAATTGCGGCCAGAAGCCATAAAACAGCGGCGCATAGCCAATCGCCTGAAACGGCGCACTCAGTTCCCGCGGCATTTGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCGATGGCAGGAAGGTTAATGGTCACCCCAATCGCCACCAGCACAAAACCAGTacgacg                                                                                           >  2:356054/1‑251 (MQ=255)                                                                                                                                                            ggTCAGCGCCATCCGTCCGCCGCAGGCGATCGCAAGCACCAGCTTAAAGCGGCTCAATTGCGGCCAGAAGCCATAAAACAGCGGCGCATAGCCCATCGCCTGAAACGGCGCACTCAGTTCCCGCGGCATTTGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCGATGGCTGGAAGGTTAATGGTCACCCCAATCGCCACCAGCACAAAACCAGTACGACGATAGTGACGt                                                                                 <  1:401421/250‑1 (MQ=255)                                                                                                                                                             gTCAGCGCCATCCGTCCGACGCAGGCGATCGCAAGCACCAGCTTAAAGCGGCTCAATTGCGGCCAGAAGCCATAAAACAGCGGCGCATAGCCAATCGCCTGAAACGGCGCACTCAGTTCCCGCGGCATTTGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCGATGGCAGGAAGGTTAATGGTCACCCCAATCGCCACCAGCACAAAACCAGTACGACGATAGTGACGTaa                                                                               <  1:140906/251‑1 (MQ=255)                                                                                                                                                                aGCGCCATCCGTCCGCCGCAGGACATCAAAAGCACCAGCTTAAAGCGGCTCAATTGCGCCCAGAAGAAATAAAACAGCGGCGCATAGCAAATCGCCTGAAACGGCCAACGCAGTTACCGCGGCATTTACAGAACGAACGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCGCAGGCAGGAAGGTTAATAGCCACCCCAATCAACACCAGCACAACACCAGTACGTCGCTCGTGACGTAAGCt                                                                            >  2:29205/1‑251 (MQ=255)                                                                                                                                                                  cgcCAGCCGTCCGACGCAGGCGATCGCAAGCACCAGCTTAAAGCGGCTCAATTGCGGCCAGAAGCCATAAAACAGCGGCGCATAGCCAATCGCCTGAAACGGCGCACTCAGTTCCCGCGGCATTTGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCGATGGCAGGAAGGTTAATGGTCACCCCAATCGCCACCAGCACAAAACCAGTACGACGATAGTGACGTAAGCTGa                                                                          <  2:41913/251‑1 (MQ=255)                                                                                                                                                                      aTCCGTCCGACGCAGGCGATCGCAAGCACCAGCTTAAAGCGGCTCAATTGCGGCCAGAAGCCATAAAACAGCGGCGCATAGCCAATCGCCTGAAACGGCGCACTCAGTTCCCGCGGCATTTGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCGATGGCAGGAAGGTTAATGGTCACCCCAATCGCCACCAGCACAAAACCAGTACGACTATAGTGACGTAAGCTGAACTg                                                                      <  2:317058/251‑1 (MQ=255)                                                                                                                                                                      aTCCGTCCGACGCAGGCGATCGCAAGCACCAGCTTAAAGCGGCTCAATTGCGACCAGACCCCATAAAACAGCGGCGCATAGCCAATCGCCTGAAACGGCCCACTCCGTTCCCGCGCCATTTGAAGTACGCCGGCACACCAGCGATCCGCCCAGTCCAGCTGCCATTGCAGGGCGATGGCCGGAAGGTTAATGGTCACCCCAATCGCAACCAGCACAACACCAGACCGACGATAGTGACGTAAGCTGAACTg                                                                      >  1:65206/1‑251 (MQ=255)                                                                                                                                                                           tccgACGCAGGCGATCGCAAGCACCAGCTTAAAGCGGCTCAATTGCGGCCAGAAGCCATAAAACAGCGGCGCATAGCCAATCGCCTGAAACGGCGCACTCAGTTCCCGCGGCATTTGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCGATGGCAGGAAGGTTAATGGTCACCCCAATCGCCACCAGCACAAAACCAGTACGACGATAGTGACGTAAGCTGAACTGCCCtt                                                                 >  2:315394/1‑251 (MQ=255)                                                                                                                                                                                            cAAGCACCAGCTTAAAGCGGCTCAATTGCGGCCAGAAGCCATAAAACAGCGGCGCATAGCCAATCGCCTGAAACGGCGCACTCAGTTCCCGCGGCATTTGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCGATGGCAGGAAGGTTAATGGTCACCCCAATCGCCACCAGCACAAAACCAGTACGACGATAGTGACGTAAGCTGAACTGCCCTTTCAGCCAGCCGCTgcgc                                                >  1:457/1‑251 (MQ=255)                                                                                                                                                                                                              ggCTCAATTGCGGCCAGAAGCCATAAAACAGCGGCGCATAGCCAATCGCCTGAAACGGCGCACTCAGTTCCCGCGGCATTTGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCGATGGCAGGAAGGTTAATGGTCACCCCAATCGCCACCAGCACAAAACCAGTACGACGATAGTGACGTAAGCTGAACTGCCCTTTCAGCCAGCCGCTGCGCATCAGCGCCGCACCAATg                              <  1:237653/251‑1 (MQ=255)                                                                                                                                                                                                                        cGGCCAGAAGCCATAAAACAGCGGCGCATAGCCAATCGCCTGAAACGGCGCACTCAGTTCCCGCGGCATTTGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCGATGGCAGGAAGGTTAATGGTCACCCCAATCGCCACCAGCACAAAACCAGTACGACGATAGTGACGTAAGCTGAACTGCCCTTTCAGCCAGCCGCTGCGCATCAGCGCCGCACCAATGAGCATCATcc                    >  1:355163/1‑251 (MQ=255)                                                                                                                                                                                                                         ggCCAGAAGCCATAAAACAGCGGCGCATAGCCAATCGCCTGAAACGGCGCACTCAGTTCCCGCGGCATTTGAAGTAAGAAGGCACACCAGCGATCCGCCCAGTCCAGCTGCCATTGCAGGGCGATGGCAGGAAGGTTAATGGTCACCCCAATCGCCACCAGCACAAAACCAGTACGACGATAGTGACGTAAGCTGAACTGCCCTTTCAGCCAGCCGCTGCGCATCAGCGCCGCACCAATGAGca                          <  2:126214/244‑1 (MQ=255)                                                                                                                                                                                                                         ggCCAGAAGCCATAAAACAGCGGCGCATAGCCAATCGCCTGAAACGGCGCACTCAGTTCCCGCGGCATTTGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCGATGGCAGGAAGGTTAATGGTCACCCCAATCGCCACCAGCACAAAACCAGTACGACGATAGTGACGTAAGCTGAACTGCCCTTTCAGCCAGCCGCTGCGCATCAGCGCCGCACCAATGAGCATCATccc                   >  1:374635/1‑251 (MQ=255)                                                                                                                                                                                                                         ggCCAGAAGCCATAAAACAGCGGCGCATAGCCAATCGCCTGAAACGGCGCACTCAGTTCCCGCGGCATTTGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCGATGGCAGGAAGGTTAATGGTCACCCCAATCGCCACCAGCACAAAACCAGTACGACGATAGTGACGTAAGCTGAACTGCCCTTTCAGCCAGCAGCTGCGCATCAGCGCCGCACCAATGAGca                          >  1:126214/1‑244 (MQ=255)                                                                                                                                                                                                                          gCCAGAAGCCATAAAACAGCGGCGCATAGCCAATCGCCTGAAACGGCGCACTCAGTTCCCGCGGCATTTGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCAAGCTGCCATTGCAGGGCGATGGCAGGAAGGTTAATGGTCACCCCAATCGCCACCAGCACAAAACCAGTACGACGATAGTGACGCAAGCTGAACTGCCCTTTCAGCCAGCCGCTGCGCATCAGCGCCGCACCAATGAGCATCATCCCt                  >  2:445403/1‑251 (MQ=255)                                                                                                                                                                                                                             aGAAGCCATAAAACAGCGGCGCATAGCCAATCGCCTGAAACGGCGCACTCAGTTCCCGCGGCATTTGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCGATGGCAGGAAGGTTAATGGTCACCCCAATCGCCACCAGCACAAAACCAGTACGACGATAGTGACGTAAGCTGAACTGCCCTTTCAGCCAGCCGCTGCGCATCAGCGCCGCACCAATGAGCATCATCCCTGcc               >  1:353602/1‑251 (MQ=255)                                                                                                                                                                                                                               aaGCCATAAAACAGCGGCGCATAGCCAATCGCCTGAAACGGCGCACTCAGTTCCCGCGGCATTTGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCGATGGCAGGAAGGTTAATGGTCACCCCAATCGCCACCAGCACAAAACCAGTACGACGATAGTGACGTAAGCTGAACTGCCCTTTCAGCCAGCCGCTGCGCATCAGCGCCGCACCAATGAGCATCATCCCTGCCAg             <  1:250358/251‑1 (MQ=255)                                                                                                                                                                                                                                      aaaaCAGCGGCGCATAGCCAATCGCCTGAAACGGCGCACTCAGTTCCCGCGGCATTTGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCGATGGCAGGAAGGTTAATGGTCACCCCAATCGCCACCAGCACAAAACCAGTACGACGATAGTGACGTAAGCTGAACTGCCCTTTCAGCCAGCCGCTGCGCATCAGCGCCGCACCAATGAGCATCATCCCTGCCAGTTgcc        >  1:41211/1‑249 (MQ=255)                                                                                                                                                                                                                                          cAGCGGCGCATAGCCAATCGCCTGAAACGGCGCACTCAGTTCCCGCGGCATTTGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCGATGGCAGGAAGGTTAATGGTCACCCCAATCGCCACCAGCACAAAACCAGTACGACGATAGTGACGTAAGCTGAACTGCCCTTTCAGCCAGCCGCTGCGCATCAGCGCCGCACCAATGAGCATCATCCCTGCCAGTTGCCAGCCAt  <  2:349630/251‑1 (MQ=255)                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                      CCCTGGCGGAAGTAACGCAGCCAGATAACAGAGAAGAGGATATTCGCCAGCCATACCGGAATAACAAACGCCAGCAGCTCCAGGCGGTCAAAATGCATAAACAAACCGAGGTGGTAAAAAAGCGTGGTACAAATCAGCGTTTGCAATAGATAGTTGGTCAGCGCCATCCGTCCGACGCAGGCGATCGCAAGCACCAGCTTAAAGCGGCTCAATTGCGGCCAGAAGCCATAAAACAGCGACGCATAGCCAATCGCCTGAAACGGCGCACTCAGTTCCCGCGGCATTTGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCGATGGCAGGAAGGTTAATGGTCACCCCAATCGCCACCAGCACAAAACCAGTACGACGATAGTGACGTAAGCTGAACTGCCCTTTCAGCCAGCCGCTGCGCATCAGCGCCGCACCAATGAGCATCATCCCTGCCAGTTGCCAGCCAT  >  NC_000913/2241883‑2242366

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑