Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,175,952	G→A	G176R (GGG→AGG)	lolC →	lipoprotein release complex ‑ inner membrane subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,175,952	0	G	A	100.0%	163.6 / NA	48	G176R (GGG→AGG)	lolC	lipoprotein release complex ‑ inner membrane subunit
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (21/27);  total (21/27)

TATTACTACCGGTGATGTGGTACTGCAAAGCGCGCGCAGCGTGGCGGTCGGGGTGATGCTCGGTATCGACCCGGCGCAAAAAGATCCACTTACACCGTATCTGGTCAATGTGAAACAAACTGACCTCGAGCCGGGGAAATATAATGTCATCCTCGGCGAACAACTTGCCTCACAGCTAGGCGTTAATCGCGGTGATCAAATCCGCGTGATGGTACCATCTGCCAGCCAGTTCACGCCGATGGGGCGTATTCCAAGCCAGCGCCTGTTCAATGTGATTGGCACTTTCGCCGCCAACAGTGAAGTCGATGGCTATGAAATGCTGGTGAATATTGAGGATGCCTCGCGTCTGATGCGTTATCCGGCAGGCAATATTACCGGCTGGCGTTTGTGGCTGGATGAGCCGCTGAAAGTCGACTCATTAAGTCAGCAAAAACTGCCTGAAGGCAGCAAATGGCAGGACTGGCGTGATCGTAAAGGCGAGTTGTTC  >  NC_000913/1175711‑1176197                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                      tATTAATACCGGTGATGTGGTACTGCAAAGCGCGCGCAGCGTGGCGGTCGGGGTGATGCTCGGTATCGACCCGGCGCAAAAAGATCCACTTACCCCGTATCTGGTCCATGTGAAACAAACTGACCTCGAGCCGGGGAAATATAATGTCATCCTCGGCGAACAACTTGCCTCACAGCTAGGCGTTAATCGCGGGGATCAAATCCGCGTGATGGTACCATCTGCCAGCCAGTTCACGCCGATGAGGCGTATTc                                                                                                                                                                                                                                              >  1:343938/1‑251 (MQ=255)                           aaaGCGCGCGCAGCGTGGCGGTCGGGGTGATGCTCGGTATCGACCCGGCGCAAAAAGATCCACTTACACCGTATCTGGTCAATGTGAAACAAACTGACCTCGAGCCGGGGAAATATAATGTCATCCTCGGCGAACAACTTGCCTCACAGCTAGGCGTTAATCGCGGTGATCAAATCCGCGTGATGGTACCATCTGCCAGCCAGTTCACGCCGATGAGGCGTATTCCAAGCCAGCGCCTGTTCAATGTGAtt                                                                                                                                                                                                                    >  2:172244/1‑251 (MQ=255)                            aaGCGCGCGCAGCGTGGCGGTCGGGGTGATGCTCGGTATCGACCCGGCGCAAAAAGATCCACTTACACCGTATCTGGTCAATGTGAAACAAACTGACCTCGAGCCGGGGAAATATAATGTCATCCTCGGCGAACAACTTGCCTCACAGCTAGGCGTTAATCGCGGTGATCAAATCCGCGTGATGGTACCATCTGCCAGCCAGTTCACGCCGATGAGGCGTATTCCAAGCCAGCGCCTGTTCAATGTGATTg                                                                                                                                                                                                                   <  1:132475/251‑1 (MQ=255)                                                   gggNGATGCTCGGTATCGACCCGGCGCAAAAAGATCCACTTACACCGTATCTGGTCAATGTGAAACAAACTGACCTCGAGCCGGGGAAATATAATGTCATCCTCGGCGAACAACTTGCCTCACAGCTAGGCGTTAATCGCGGTGATCAAATCCGCGTGATGGTACCATCTGCCAGCCAGTTCACGCCGATGAGGCGTATTCCAAGCCAGCGCCTGTTCAATGTGATTGGCACTTTCGCCGCCAACAGTGaa                                                                                                                                                                                            <  2:416193/251‑1 (MQ=255)                                                       gATGCTCGGTATCGACCCGGCGCAAAAAGATCCACTTACACCGTATCTGGTCAATGTGAAACAAACTGACCTCGAGCCGGGGAAATATAATGTCATCCTCGGCGAACAACTTGCCTCACAGCTAGGCGTTAATCGCGGTGATCAAATCCGCGTGATGGTACCATCTGCCAGCCAGTTCACGCCGATGAGGCGTATTCCAAGCCAGCGCCTGTTCAATGTGATTGGCACTTTCGCCGCCAACAGTGAAGTCg                                                                                                                                                                                        <  1:172244/251‑1 (MQ=255)                                                             cGGTATCGACCCGGCGCAAAAAGACCCACTTACACCGTATCTGGTCAATGTGAAACAAACTGACCTCGAGCCGGGGAAATATAATGTCATCCTCGGCGAACAACTTGCCTCACAGCTAGGCGTTAATCGCGGTGATCAAATCCGCGTGATGGTACCATCTGCCAGCCAGTTCACGCCGATGAGGCGTATTCCAAGCCAGCGCCTGTTCAATGTGATTGGCACTTTCGCCGCCAACAGTGAAGTCGATgg                                                                                                                                                                                    <  2:299753/249‑1 (MQ=255)                                                               gTATCGACCCGGCGCAAAAAGATCCACTTACACCGTATCTGGTCAATGTGAAACAAACTGACCTCGAGCCGGGGAAATATAATGTCATCCTCGGCGAACAACTTGCCTCACAGCTAGGCGTTAATCGCGGTGATCAAATCCGCGTGATGGTACCATCTGCCAGCCAGTTCACGCCGATGAGGCGTATTCCAAGCCAGCGCCTGTTCAATGTGATTGGCACTTTCGCCGCCAACAGTGAAGTCGATGGCTAt                                                                                                                                                                                <  1:220106/251‑1 (MQ=255)                                                                 aTCGACCCGGCGCAAAAAGATCCACTTACACCGTATCTGGTCAATGTGAAACAAACTGACCTCGAGCCGGGGAAATATAATGTCATCCTCGGCGAACAACTTTCCTCACAGCTAGGCGTTAATCGCGGTGATCAAATCCGCGTGATGGTTCCATCTGCCAGCCAGTTCACGCCGATGAGGCGTATTCCAAGCCAGCGCCTGTTCAATGTGATTGGCACTTTCGCCGCCAACAGTGAAGTCGATGGCTATg                                                                                                                                                                               <  2:268664/250‑1 (MQ=255)                                                                            gcAAAAAGATCCACTTACACCGTATCTGGTCAATGTGAAACAAACTGACCTCGAGCCGGGGAAATATAATGTCATCCTCGGCGAACAACTTGCCTCACAGCTAGGCGTTAATCGCGGTGATCAAATCCGCGTGATGGTACCATCTGCCAGCCAGTTCACGCCGATGAGGCGTATTCCAAGCCAGCGCCTGTTCAATGTGATTGGCACTTTCGCCGCCAACAGTGAAGTCGATGGCTATGAAATGCTGGTg                                                                                                                                                                    >  2:216384/1‑250 (MQ=255)                                                                              aaaaaGATCCACTTACACCGTATCTGGTCAATGTGAAACAAACTGACCTCGAGCCGGGGAAATATAATGTCATCCTCGGCGAACAACTTGCCTCACAGCTAGGCGTTAATCGCGGTGATCAAATCCGCGTGATGGTACCATCTGCCAGCCAGTTCACGCCGATGAGGCGTATTCCAAGCCAGCGCCTGTTCAATGTGATTGGCACTTTCGCCGCCAACAGTGAAGTCGATGGCTATGAAATGCTGGTGAat                                                                                                                                                                 >  1:454684/1‑251 (MQ=255)                                                                                aaaGATCCACTTACACCGTATCTGGTCAATGTGAAACAAACTGACCTCGAGCCGGGGAAATATAATGTCATCCTCGGCGAACAACTTGCCTCACAGCTAGGCGTTAATCGCGGTGATCAAATCCGCGTGATGGTACCATCTGCCAGCCAGTTCACGCCGATGAGGCGTATTCCAAGCCAGCGCCTGTTCAATGTGATTGGCACTTTCGCCGCCAACAGTGAAGTCGATGGCTATGAAATGCTGGTGAatat                                                                                                                                                               <  1:297007/251‑1 (MQ=255)                                                                                    aTCCACTTACACCGTATCTGGTCAATGTGAAACAAACTGACCTCGAGCCGGGGAAATATAATGTCATCCTCGGCGAACAACTTGCCTCACAGCTAGGCGTTAATCGCGGTGATCAAATCCGCGTGATGGTACCATCTGCCAGCCAGTTCACGCCGATGAGGCGTATTCCa                                                                                                                                                                                                                                            >  1:196098/1‑170 (MQ=255)                                                                                    aTCCACTTACACCGTATCTGGTCAATGTGAAACAAACTGACCTCGAGCCGGGGAAATATAATGTCATCCTCGGCGAACAACTTGCCTCACAGCTAGGCGTTAATCGCGGGGATCAAATCCGCGTGATGGTACCATCTGCCAGCCAGTTCACGCCGATGAGGCGTATTCCa                                                                                                                                                                                                                                            <  2:196098/170‑1 (MQ=255)                                                                                     tCCACTTACACCGTATCTGGTCAATGTGAAACAAACTGACCTCGAGCCGGG‑AAATATAATGTCATCCTCGGCGAACAACTTGCCTCACAGCTAGGCGTTAATCGCGGTGATCAAATCCGCGTGATGGAACCATCTGCCAGCCAGTTCACGCCGCTGAGGCGTATTCCAAGCCAGCGCCTGTTCAATGTGATTGGCACTTTCGCCGCCAACAGTGAAGTCGATGGCTATGAAATGCTGGTGAATATTGAgg                                                                                                                                                          >  1:390256/1‑250 (MQ=255)                                                                                            acacCGTATCTGGTCAATGTGAAACAAACTGACCTCGAGCCGGGGAAATATAATGTCATCCTCGGCGAACAACTTGCCTCACAGCTAGGCGTTAATCGCGGTGATCAAATCCGCGTGATGGTACCATCTGCCAGCCAGTTCACGCCGATGAGGCGTATTCCAAGCCAGCGCCTGTTCAATGTGATTGGCACTTTCGCCGCCAACAGTGAAGTCGATGGCTATGAAATGCTGGTGAATATTGAGGATGCCTc                                                                                                                                                   >  1:63262/1‑251 (MQ=255)                                                                                                       ggTCAATGTGAAACAAACTGACCTCGAGCCGGGGAAATTTAATGTCATCCTCGGCGAACAACTTGCCTCACAGCTAGGCGTTAATCGCGGTGATCAAATCCGCGTGATGGTACCATCTGCCAGCCAGTTCACGCCGATGAGGCGTATTCCAAGCCAGCGCCTGTTCAATGTGATTGGCACTTTCGCCGCCAACAGTGAAGTCGATGGCTATGAAATGCTGGTGAATATTGAGGATGCCTCGCGTCTGAt                                                                                                                                          <  1:110492/249‑1 (MQ=255)                                                                                                           aaTGTGAAACAAACTGGCCTCGAGCCGGGGAAATATAATGTCATCCTCGGCGAACAACTTGCCTCACAGCTAGGCGTTAATCGCGGTGATCAAATCCGCGTGATGGTACCATCTGCCAGCCAGTTCACGCCGATGAGGCGTATTCCAAGCCAACGCCTGTTCAATGTGATTGGCACTTTCGCCGCCAACAGTGAAGTCGATGGCTATGAAATGCTGGTGAATATTGAGGATGCCTCGCGTCTGATGCGtt                                                                                                                                     >  2:14705/1‑250 (MQ=255)                                                                                                                     aaacTGACCTCGAGCCGGGGAAATATAATGTCATCCTCGGCGAACAACTTGCCTCACAGCTAGGCGTTAATCGCGGTGATCAAATCCGCGTGATGGTACCATCTGCCAGCCAGTTCACGCCGATGAGGCGTATTCCAAGCCAGCGCCTGTTCAATGTGATTGGCACTTTCGCCGCCAACAGTGAAGTCGATGGCTATGAAATGCTGGTGAATATTGAGGATGCCTCGCGTCTGATGCGTTATCCggcaggc                                                                                                                          >  2:30677/1‑251 (MQ=255)                                                                                                                            ccTCGAGCCGTGGAAATATAATGTCATCCACGGCGAACAACTGGCCTCACAGCTAGGCGTTAATCGCGGTTATCAAATCCGCGTGATGGTCCCATCTGCCCGCCAGTGCTCGCCGATTATTTGTATTCCAAGCCAGCGCCTGTGCAATGTTATTGGCACTTTCGCCGCCAACAGTGAAGTCGATGGCTATGAAATGCTGGTGAATATTGAGGATGCCTCGCGTCTGATGCGTTATCCTGCAGGCAATATTa                                                                                                                   <  2:458135/251‑1 (MQ=255)                                                                                                                                    cGGGGAAATATAATGTCATCCTCGGCGAACAACTTGCCTCACAGCTAGGCGTTAATCGCGGTGATCAAATCCGCGTGATGGTACCATCTGCCAGCCAGTTCACGCCGATGAGGCGTATTCCAAGCCAGCGCCTGTTCAATGTGATTGGCACTTTCGCCGCCAACAGTGAAGTCGATGGCTATGAAATGCTGGTGAATATTGAGGATGCCTCGCGTCTGATGCGTTATCCGGCAGGCAATATTACCGGCTgg                                                                                                           >  1:79571/1‑251 (MQ=255)                                                                                                                                     ggggAAATATAATGTCATCCTCGGCGAACAACTTGCCTCACAGCTAGGCGTTAATCGCGGTGATCAAATCCGCGTGATGGAACCATCTGCCAGCCAGTTCACGCCGATGAGGCGTATTCCAAGCCAGCGCCTGTTCAATGTGATTGGCACTTTCGCCGCCAACAGTGAAGTCGATGGCTATGAAATGCTGGTGAATATTGAGGATGCCTCGCGTCTGATGCGTTATCCGGCAGGCAATATTACCGGCTGGc                                                                                                          >  2:359076/1‑251 (MQ=255)                                                                                                                                                aTGTCATCCTCGGCGAACAACTTGCCTCACAGCTAGGCGTTAATCGCGGTGATCAAATCCGCGTGATGGTACCATCTGCCAGCCAGTTCACGCCGATGAGGCGTATTCCAAGCCAGCGCCTGTTCAATGTGATTGGCACTTTCGCCGCCAACAGTGAAGTCGATGGCTATGAAATGCTGGTGAATATTGAGGATGCCTCGCGTCTGATGCGTTATCCGGCAGGCAATATTACCGGCTGGCGTTTGTGGCTg                                                                                               <  1:293230/251‑1 (MQ=255)                                                                                                                                                  gTCATCCTCGGCGACCAACTTGCCTCACAGCTAGGCGTTAATCGCGGTGATAAAATCCGCGTGATGGTACCATCGGCCAGCCAGTTCACGCCGATGAGGCGTATTCCAAGCCAGCGCCTGTTCAATGTGATTGGCACTTTCGCCGCCAACAGTGAAGTCGATGGCTATGAAATGCTGGTGAATATTGAGGATGCCTCGCGTCTGATGCGTTATCCGGCAGGCAATATTACCGGCTGGCGTTTGTGGCTGGa                                                                                             <  2:343938/251‑1 (MQ=255)                                                                                                                                                     aTCCTCGGCGAACAACTTGCCTCACAGCTAGGCGTTAATCGCGGTGATCAAATCCGCGTGATGGTACCATCTGCCAGCCAGTTCACGCCGATGAGGCGTATTCCAAGCCAGCGCCTGTTCAATGTGATTGGCACTTTCGCCGCCAACAGTGAAGTCGATGGCTATGAAATGCTGGTGAatat                                                                                                                                                               <  2:370168/182‑1 (MQ=255)                                                                                                                                                     aTCCTCGGCGAACAACTTGCCTCACAGCTAGGCGTTAATCGCGGTGATCAAATCCGCGTGATGGTACCATCTGCCAGCCAGTTCACGCCGATGAGGCGTATTCCAAGCCAGCGCCTGTTCAATGTGATTGGCACTTTCGCCGCCAACAGTGAAGTCGATGGCTATGAAATGCTGGTGAatat                                                                                                                                                               >  1:370168/1‑182 (MQ=255)                                                                                                                                                            gCGAACAACTTGCCTCACAGCTAGGCGTTAATCGCGGTGATCAAATCCGCGTGATGGTACCATCTGCCAGCCAGTTCACGCCGATGAGGCGTATTCCAAGCCAGCGCCTGTTCAATGTGATTGGCACTTTCGCCGCCAACAGTGAAGTCGATGGCTATGAAATGCTGGTGAATATTGAGGATGCCTCGCGTCTGATGCGTTATCCGGCAGGCAATATTACCGGCTGGCGTTTGTGGCTGGATGAGCCGCTg                                                                                   <  1:282601/251‑1 (MQ=255)                                                                                                                                                               aacaacTTGCCTCACAGCTAGGCGTTAATCGCGGTGATCAAATCCGCGTGATGGTACCATCTGCCAGCCAGTTCACGCCGATGAGGCGTATTCCAAGCCAGCGCCTGTTCAATGTGATTGGCACTTTCGCCGCCAACAGTGAAGTCGATGGCTATGAAATGCTGGTGAATATTGAGGATGCCTCGCGTCTGATGCGTTATCCGGCAGGCAATATTACCGGCTGGCGTTTGTGGCTGGATGAGCCGCTGaa                                                                                 >  1:325405/1‑250 (MQ=255)                                                                                                                                                                  aacTTGCCTCACAGCTCGGCGTTAATCGCGGTGATCAAATCCGCGTGATGGTACCATCTGCCAGCCAGTTCACGCCGATGAGGCGTATTCCAAGCCAGCGCCTGTTCAATGTGATTGGCACTTTCGCCGCCAACAGTGAAGTCGATGGCTATGAAATGCTGGTGAATATTGAGGATGCCTCGCGTCTGATGCGTTATCCGGCAGGCAATATTACCGGCTGGCGTTTGTGGCTGGATGAGCCGCTGAAAGTc                                                                             >  1:99529/1‑251 (MQ=255)                                                                                                                                                                   acTTGCCTCACATCGAGGCGTTAATCGCGGTGATCAAATCCGCGTGATGGTACCATCTGCCAGCCATTTCACGCCGATGAGGCGTATTCCAAGCCAGCGCCTGTTCAATGTGATTGGCACTGTCGCCGCCAACAGTGAAGTCGATGGCTATGAAATGCTGGTGAATATTGAGGATTCCTCGCGTCTGATGCGTTATCCGGCAGTCAATATTACCGGCTGGCGTTTGTGGCTGGATGAGCCGCTGAAAGTCg                                                                            <  1:300836/251‑1 (MQ=255)                                                                                                                                                                      tGCCTCACAGCTAGGCGTTAATCGCGGTGATCAAATCCGCGTGATGGTACCATCTGCCAGCCAGTTCACGCCGATGAGGCGTATTCCAAGCCAGCGCCTGTTCAATGTGATTGGCACTTTCGCCGCCAACAGTGAAGTCGATGGCTATGAAATGCTGGTGAATATTGAGGATGCCTCGCGTCTGATGCGTTATCCGGCAGGCAATATTACCGGCTGGCGTTTGTGGCTGGATGAGCCGCTGAAAGTCGa                                                                           <  1:89721/249‑1 (MQ=255)                                                                                                                                                                                 tAGGCGTTAATCGCGGTGATCAAATCCGCGTGATGGTACCATCTGCCAGCCAGTTCACGCCGATGAGGCGTATTCCAAGCCAGCGCCTGTTCAATGTGATTGGCACTTTCGCCGCCAACAGTGAAGTCGATGGCTATGAAATGCTGGTGAATATTGAGGATGCCTCGCGTCTGATGCGTTATCCGGCAGGCAATATTACCGGCTGGCGTTTGTGGCTGGATg                                                                                           <  1:52326/222‑1 (MQ=255)                                                                                                                                                                                 tAGGCGTTAATCGCGGTGATCAAATCCGCGTGATGGTACCATCTGCCAGCCAGTTCACGCCGATGAGGCGTATTCCAAGCCAGCGCCTGTTCAATGTGATTGGCACTTTCGCCGCCAACAGTGAAGTCGATGGCTATGAAATGCTGGTGAATATTGAGGATGCCTCGCGTCTGATGCGTTATCCGGCAGGCAATATTACCGGCTGGCGTTTGTGGCTGGATGAgatcgggagagcggcgtgtagggga                                                                 >  2:52326/1‑224 (MQ=255)                                                                                                                                                                                     cGTTAATCGCGGTGATCAAATCAGCGTGATGGTACCATCTGCCAGCCAGTTCACGCCGATGAGGCGTATTCCAAGCCAGCGCCTGTTCAATGTGATTGGCACTTTCGCCGCCAACAGTGAAGTCGATGGCTATGAAATGCTGGTGAATATTGAGGATGCCTCGCGTCTGATGCGTTATCCGGCAGGCAATATTACCGGCTGGCGTTTGTGGCTGGGTGAGACGCTGAAAGTCGACTCATTAAGTCAGCaa                                                           >  2:175955/1‑250 (MQ=255)                                                                                                                                                                                      gTTAATCGCGGTGATCAAATCCGCGTGATGGTACCATCTGCCAGCCAGTTCACGCCGATGAGGCGTATTCCAAGCCAGCGCCTGTTCAATGTGATTGGCACTTTCGCCGCCAACAGTGAAGTCGATGGCTATGAAATGCTGGTGAATATTGAGGATGCCTCGCGTCTGATGCGTTATCCGGCAGGCAATATTACCGGCTGGCGTTTGTGGCTGGATGAGCCGCTGAAAGTCGACTCATTAAGTTAGCaaa                                                          >  1:405337/1‑250 (MQ=255)                                                                                                                                                                                             gcgGTGATCAAATCCGCGTGATGGTACCATCTGCCAGCCAGTTCACGCCGATGAGGCGTATTCCAAGCCAGCGCCTGTTCAATGTGATTGGCACTTTCGCCGCCAACAGTGAAGTCGATGGCTATGAAATGCTGGTGAATATTGAGGATGCCTCGCGTCTGATGCGTTATCCGGCAGGCAATATTACCGGCTGGCGTTTGTGGCTGGATGAGCCGCTGAAAGTCGACTCATTAAGTCAGCAAAAACTGCCt                                                  >  1:120417/1‑251 (MQ=255)                                                                                                                                                                                                gTGATCAAATCCGAGTGATGGTACCATCTGCCAGCCAGTTCACGCCGATGAGGCGTATTACAAGCCAGCGCCTGTTCAATGTGATAGGCACTTTCGCCGCCAACAGTGAAGTCGATGGCTATGAAATGCTGGTGAATATTGAGGATGCCTCGCGTCTGATGCGTTATCCGGCAGGCAATATTACCGGCTGGCGTTTGTGGCTGGGTGAGCCGCTGGAGTTCGAATTCTTAAGTGTGCAAAAACTGCCttat                                               >  2:375917/1‑248 (MQ=255)                                                                                                                                                                                                             cgTGATGGTACCCTCTGCCAGCCAGTTAACGCCGATGAGGCGTCTTCCAAGCCAGCGCCTGTTCAATGTGATTGGCACTTCCGCCGCCTACAGTGAAGTAGATGGCTATGAAATGCTGGTGAATATTGAGGATGCATCGCGTCTGATGCGTTATCCGGAAGGCACTATTACCGGCTGGCGTTTGTGGCTGGATGAGCCGCTGAAAGTCGACTCATTGAGTCAGCAAAAACTGCCTGAAGGAAGCAAAtagc                                  >  2:46073/1‑248 (MQ=255)                                                                                                                                                                                                             cgTGATGGTACCATCTGCCAGCCAGTTCACGCCGATGAGGCGTATTCCAAGCCAGCGCCTGTTCAATGTGATTGGCACTTTCGCCGCCAACAGTGAAGTCGATGGCTATGAAATGCTGGTGAATATTGAGGATGCCTCGCGTCTGATGCGTTATCCGGCAGGCAATATTACCGGCTGGCGTTTGTGGCTGGATGAGCCGCTGAAAGTCGACTCATTAAGTCAGCAAAAACTGCCTGAAGGCAGCAAATGGc                                  <  1:46645/251‑1 (MQ=255)                                                                                                                                                                                                                gATGGTACCATCTGCCAGCCAGTTCACGCCGATGAGGCGTATTCCAAGCCAGCGCCTGTTCAATGTGATTGGCACTTTCGCCGTCAACAGTGAAGTCGATGGCTATGAAATGCTGGTGAATATTGAGGATGCCTCGCGTCTGATGCGTTATCCGGCAGGCAATATTACCGGCTGGCGTTTGTGGCTGGATGAGCCGCTGAAAGTCGACTCATTAAGTCAGCAAAAACTGCCTGAAGGCAGCAAATGGCAg                                <  1:91005/250‑1 (MQ=255)                                                                                                                                                                                                                   ggttccctcggccagccaGTTCACGCCGATGAGGCGTATTCCAAGCCAGCGCCTGTTCAATGTGATTGGCACTTTCGCCGCCAACAGTGAAGTCGATGGCTATGAAATGCTGGTGAATATTGAGGATGCCTCGCGTCTGATGCGTTATCCGGCAGGCAATATTACCGGCTGGCGTTTGTGGCTGGATGAGCCGCTGAAAGTCGACTCATTAAGTCAGCAAAAACTGCCTGAAGGCAGCAAATGGCAGGAc                             <  2:297906/240‑1 (MQ=255)                                                                                                                                                                                                                      cccATCTGCCAGCCAGTTCACGCCGATGAGGCGTATTCCAAGCCAGCGCCTGTTCAATGTGATTGGCACTTTCGCCGCCAACAGTGAAGTCGATGGCTATGAAATGCTGGTGAATATTGAGGATGCCTCGCGTCTGATGCGTTATCCGGCAGGCAATATTACCGGCTGGCGTTTGTGGCTGGATGAGCCGCTGAAAGTCGACTCATTAAGTCAGCAAAAACTGCCTGAAGGCAGCAAATGGCAGGACTGGc                         <  1:175955/250‑1 (MQ=255)                                                                                                                                                                                                                        cATCTGCCATCCAGTTCACGCCGATGAGGCGTATTCCAAGCCAGCGCCTGTTCAATGTGATTGGCACTTTCGCCGCCAACAGTGAAGTCGATGGCTATGAAATGCTGGTGAATATTGAGGATGCCTCGCGTCTGATGCGTTATCCGGCAGGCAATATTACCGGCTGGCGTTTGTGGCTGGATGAGCCGCTGAAAGTCGACTCATTAAGTCAGCAAAAACTGCCTGAAGGCAGCAAATGGCAGGACTGGCGt                       <  2:373425/251‑1 (MQ=255)                                                                                                                                                                                                                        cATCTGCCAGCCAGTTCACGCCGATGAGGCGTATTCCAAGCCAGCGCCTGTTCAATGTGATTGGCACTTTCGCCGCCAACAGTGAAGTCGATGGCTATGAAATGCTGGTGAATATTGAGGATGCCTCGCGTCTGATGCGTTATCCGGCAGGCAATATTACCGGCTGGCGTTTGTGGCTGGATGAGCCGCTGAAAGTCGACTCATTAAGTCAGCAAAAACTGCCTGAAGGCAGCAAATGGCAGGACTGGCGt                       <  1:320338/251‑1 (MQ=255)                                                                                                                                                                                                                              cccgccagTTCACGCCTATGAGGCGTATTCCAAGCCAGCGCCTGTTCAATGTGATTGGCGCTTGCGCCGCCAACAGTGAAGTCGATGGCTAGGAAATGCTGGTGAATATTGAGGATGCCTCGCTGCTGATGCGTTATCCGGCAGGCAATATTACCGGCTGGCGTTTGTGGCTGGAGGAGCCTCGGAAAGTCGACTCATTGAGTCAGCAAAAACTGCCTGAAGGCAGCAAATGGCAGGACAGCCGTGATCGt                 <  2:54256/248‑1 (MQ=255)                                                                                                                                                                                                                              ccagccagTTCACGCCGATGAGGCGTATTCCAAGCCAGCGCCTGTTCAATGTGATTGGCACTTTCGCCGCCAACAGTGAAGTCGATGGCTATGAAATGCTGGTGAATATTGAGGATGCCTCGCGTCTGATGCGTTATCCGGCAGGCAATATTACCGGCTGGCGTTTGTGGCTGGATGAGCCGCTGAAAGTCGACTCATTAAGTCAGCAAAAACTGCCTGAAGGCAGCAAATGGCAGGACTGGCGTGATCGt                 <  2:366392/251‑1 (MQ=255)                                                                                                                                                                                                                                 gccagTTCACGCCGATGAGGCGTATTCCAAGCCAGCGCCTGTTCAATGTGATTGGCACTTTCGCCGCCAACAGTGAAGTCGATGGCTATGAAATGCTGGTGAATATTGAGGATGCCTCGCGTCTGATGCGTTATCCGGCAGGCAATATTACCGGCTGGCGTTTGTGGCTGGATTAGCCGCTGAAAGTCGACTCATTAAGTCAGCAAAAACTGCCTGAAGGCAGCAAATGGCAGGACTGGCGTGATCGTaa               >  2:336737/1‑250 (MQ=255)                                                                                                                                                                                                                                        cACGCCTATGAGGCGTATTCCAAGCCAGCGCCTGTTCAATGTGATTGGCACTTTCGCCGCCAACAGTGAAGTCGATGGCTATGAAATGCTGGTGAATATTGAGGATGCCTCGCGTCTGATGCGTTATCCGGCAGGCAATATTACCGGCTGGCGTTTGTGGCTGGATGAGCCGCTGAAAGTCGGCTCATTAAGTCAGCAAAAACTGCCTGAAGGCAGCAAATGGCAGGACTGGCGTGATCGTAAAGGCGAgt       <  2:344680/251‑1 (MQ=255)                                                                                                                                                                                                                                             cGATGAGGCGTATTCCAAGCCAGCGCCTGTTCAATGTGATTGGCACTTTCGCCGCCAACAGTGAAGTCGATGGCTATGAAATGCTGGTGAATATTGAGGATGCCTCGCGTCTGATGCGTTATCCGGCAGGCAATATTACCGGCTGGCGTTTGTGGCTGGATGAGCCGCTGAAAGTCGACTCATTAAGTCAGCAAAAACTGCCTGAAGGCAGCAAATGGCAGGACTGGCGTGATCGTAAAGGCGAGTTGTTc  <  2:454684/251‑1 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                      TATTACTACCGGTGATGTGGTACTGCAAAGCGCGCGCAGCGTGGCGGTCGGGGTGATGCTCGGTATCGACCCGGCGCAAAAAGATCCACTTACACCGTATCTGGTCAATGTGAAACAAACTGACCTCGAGCCGGGGAAATATAATGTCATCCTCGGCGAACAACTTGCCTCACAGCTAGGCGTTAATCGCGGTGATCAAATCCGCGTGATGGTACCATCTGCCAGCCAGTTCACGCCGATGGGGCGTATTCCAAGCCAGCGCCTGTTCAATGTGATTGGCACTTTCGCCGCCAACAGTGAAGTCGATGGCTATGAAATGCTGGTGAATATTGAGGATGCCTCGCGTCTGATGCGTTATCCGGCAGGCAATATTACCGGCTGGCGTTTGTGGCTGGATGAGCCGCTGAAAGTCGACTCATTAAGTCAGCAAAAACTGCCTGAAGGCAGCAAATGGCAGGACTGGCGTGATCGTAAAGGCGAGTTGTTC  >  NC_000913/1175711‑1176197

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑