Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	854,523	G→A	V293M (GTG→ATG)	ybiR →	putative transporter YbiR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	854,523	0	G	A	100.0%	163.1 / NA	48	V293M (GTG→ATG)	ybiR	putative transporter YbiR
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (16/32);  total (16/32)

TTCTGACGGCGCTGGAGTTCAAACAAGAGCTGTGGGGACTGGTGATTGTGGCGGCAGGCTTTGCGCTGCTGGCACGTCGCGTGGTGCTCAGTGTGGACTGGACGCTGCTGCTGGTGTTTATGGCGATGTTTATCGACGTCCATTTACTGACCCAGCTTCCAGCGTTGCAAGGCGTGTTGGGTAACGTGAGTCATCTATCTGAACCCGGGTTATGGTTAACGGCAATCGGTTTATCGCAGGTGATCAGTAACGTGCCGAGTACCATATTGTTGCTGAACTATGTGCCGCCGTCTTTATTACTGGTATGGGCGGTAAACGTAGGTGGCTTTGGGTTATTACCCGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCA  >  NC_000913/854284‑854760                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                              ttCTGACGGCGCTGGAGTTCAAACAAGAGCTGTGGGGACTGGTGATTGTGGCGGCAGGCTTTGCGCTGCTGGCACGTCGCGTGGTGCTCAGTGTGGACTGGACGCTGCTGCTGGTGTTTATGGCGATGTTTATCGACGTCCATTTACTGACCCAGCTTCCAGCGTTGCAAGGCGTGTTGGGTAACGTGAGTCGTCTATCTGAACCCGGGTTATGGTTAACGGCAATCGGTTTATCGCAGATGATCAGTaa                                                                                                                                                                                                                                     <  2:51890/250‑1 (MQ=255)      aCGGCGCTGGAGTTCAAACAAGAGCTGTGGGGACTGGTGATTGTGGCGGCAGGCTTTGCGCTGCTGGCACGTCGCGTGGTGCTCAGTGTGGACTGGACGCTGCTGCTGGTGTTTATGGCGATGTTTATCGACGTCCATTTACTGACCCAGCTTCCAGCGTTGCAAGGCGTGTTGGGTAACGTGAGTCATCTATCTGAACCCGGGTTATGGTTAACGGCAATCGGTTTATCGCAGATGATCAGTAACGTGcc                                                                                                                                                                                                                               >  2:455965/1‑251 (MQ=255)             tGGAGTTCAAACAAGAGCTGTGGGGACTGGTGATTGTGGCGGCAGGCTTTGCGCTGCTGGGCCGGCGCGGGGTGCTCCGGGTGGACTGGACGCTTGTGGTGGGGTTTATGGCGGGGGTTATCGACGTCCCATTACTGGCCCAGCTTCCAGCGTTTCAAGGCGTGTTGGGGAAAGTGGGTCATCTATCTTAACCCGGGTTATGGTTAAAGGAACTCGGTTTATCGCAGATGGTCCGTAACGTGCCGAggac                                                                                                                                                                                                                         >  2:264403/1‑247 (MQ=255)             tGGAGTTCAAACAAGAGCTGTGGGGACTGGTGATTGTGGCGGCAGGCTTTGCGCTGCTGGCACGTCGCGTGGTGCTCAGTGTGGACTGGACGCTGCTGCTGGTGTTTATGGCGATGTTTATCGACGTCCATTTACTGACCCAGCTTCCAGCGGTGCAAGGCGTGTTGGGTAACGTGAGTCATCTATCTTAACCCGGGTTATGGTTAACGGCAATCGGTTTATCGCAGATGATCAGTAACGTGCCGAGTAcc                                                                                                                                                                                                                        >  2:146745/1‑251 (MQ=255)              ggAGTTCAAACAAGAGCTGTGGGGACTGGTGATTGTGGCGGCAGGCTTTGCGCTGCTGGCACGTCGCGTGGTGCTCAGTGTGGACTGGACGCTGCTGCTGGTGTTTATGGCGATGTTTATCGACGTCCATTTACTGACCCAGCTTCCAGCGTTGCAAGGCGTGTTGGGTAACGTGAGTCATCTATCTGAACCCGGGTTATGGTTAACGGCAATCGGTTTATCGCAGATGATCAGTAACGTGCCGAGTAcc                                                                                                                                                                                                                        <  2:121579/250‑1 (MQ=255)                        cAAGAGCTGTGGGGACTGGTGATTGTGGCGGCAGGCTTTGCGCTGCTGGCACGTCGCGTGGTGCTCAGTGTGGACTGGACGCTGCTGCTGGTGTTTATGGCGATGTTTATCGACGTCCATTTACTGACCCAGCTTCCAGCGTTGCAAGGCGTGTTGGGTAACGTGAGTCATCTATCTGAACCCGGGTTATGGTTAACGGCAATCGGTTTATCGCAGATGATCAGTAACGTGCCGAGTACCATATTGTTGCt                                                                                                                                                                                                             <  1:161679/251‑1 (MQ=255)                            agCTGTGGGGACTGGTGATTGTGGCGGCAGGCTTTGCGCTGCTGGCACGTCGCGTGGTGCTCAGTGTGGACTGGACGCTGCTGCTGGTGTTTATGGCGATGTTTATCGACGTCCATTTACTGACCCAGCTTCCAGCGTTGCAAGGCGTGTTGGGTAACGTGAGTCATCTATCTGCACCCGGGTTATGGTTAACGGCAATCGGGTTATCGCAGATGATCAGTAACGTGCCGTGTACCATATTGTTGCTGAAc                                                                                                                                                                                                         >  2:57895/1‑251 (MQ=255)                                  ggggACTGGTGATTGTGGCGGCAGGCTTTGCGCTGCTGGCACGTCGCGTGGTGCTCAGTGTGGACTGGACGCTGCTGCTGGTGTTTATGGCGATGTTTATCGACGTCCATTTACTGACCCAGCTTCCAGCGTTGCAAGGCGTGTTGGGTAACGTGAGTCATCTATCTGAACCCGGGTTATGGTTAACGGCAATCGGTTTATCGCAGATGATCAGTAACGTGCCGAGTACCATATTGTTGCTGAACTAtgt                                                                                                                                                                                                    <  2:441919/250‑1 (MQ=255)                                         ggTGATTGTGGCGACACGCTTTGCGCTGCTGGCACGCCGCGTGGCGCTCCGTGTGGACTGGACGCTGCTGCTGGTGTTTATGGCGATGCTTATTGACGTCCATTTACTGACCCAGCTTCCCGCGTTGCAAGACGAGTTGGGTAACGTAAGTCATCTATCTGAACCCGGGTTATGGTTAACGGCAATCGGTTTATCGCAGATGATCCGTAACGTGCCGAGTCCCACATTGTTGCTGAATTATCTGCCGCCGt                                                                                                                                                                                            >  2:450782/1‑251 (MQ=255)                                            gATTGTGGCGGCAGGCTTTGCGCTGCTGGCCCGTCGCGTTGTGCTCAGTGTGGACTGGACGCTGCTGATGGGGTTTATGGCGAAGTTTATCGACGGCCATTTACTGACCCAGCTTCCAGCGGTTCAAGGCGTGTTGGGTAACGTGGGTCAGCTGTCTTAACCCGGGTTATGGTTAACGGCAATCGGTGTATCGCAAATGGTCAGTAACGTGCCGAGTACCAGATTGTTGCTGAACTATGTTCCGCCGTCtt                                                                                                                                                                                         >  2:328373/1‑251 (MQ=255)                                                         ggCTTTGCGCTGCTGGCACGTCGCGTGGTGCTCAGTGTGGACTGGACGCTGCTGCTGGTGTTTATGGCGATGTTTATCGACGTCCATTTACTGACCCAGCTTCCAGCGTTACAAGGCGTGTTGGGTAACGTGAGTCATCTATCTGAACCCGGGTTATGGTTAACGGCAATCGGTTTATCGCAGATGATCAGTAACGTGCCGAGTACCATATTGTTGCTGAACTATGTGCCGCCGTCTTTATTACTGGTATg                                                                                                                                                                            >  1:305266/1‑251 (MQ=255)                                                             ttGCGCTGCTGGCACGTCGCGTGGTGCTCAGTGTGGACTGGACGCTGCTGCTGGTGTTTATGGCGATGTTTATCGACGTCCATTTACTGACCCAGCTTCCAGCGTTGCAAGGCGTGTTGGGTAACGTGAGTCATCTAGCTGAACCCGGGTTATGGTTAACGGCAATCGGTTTATCGCAGATGATCAGTAACGTGCCGAGTACCATATTGTTGCTGAACTATGTGCCGCCGTCTTTATTACTGGTATggg                                                                                                                                                                          <  1:15021/249‑1 (MQ=255)                                                                    gctgGCACGTCGCGTGGTGCTCAGTGTGGACTGGACGCTGCTGCTGGTGTTTATGGCGATGTTTATCGACGTCCATTTACTGACCCAGCTTCCAGCGTTGCAAGGCGTGTTGGGTAACGTGAGTCATCTATCTGAACCCGGGTTATGGTTAACGGCAATCGGTTTATCGCAGATGATCAGTAACGTGCCGAGTACCATATTGTTGCTGAACTATGTGCCGCCGTCTTTATTACTGGTATGGGCGGTAAAc                                                                                                                                                                  <  1:234082/250‑1 (MQ=255)                                                                          aCGTCGCGTGGTGCTCAGTGTGGACTGGACGCTGCTGCTGGTGGTCATGGCGATGTTTATCGACGTCCATTTACTGACCCAGCTTCCAGCGTTGCAAGGCGTGTTGGGTAACGTGAGTCATCTATCTGAACCCGGGTTATGGTTAACGGCAATCGGTTTATCGCAGATGATCAGTAACGTGCCGAGTACCATATTGTTGCTGAACTATGTGCCGCCGTCTTTATTACTGGTATGGGCGGTAAACGTAGGTg                                                                                                                                                           <  1:328373/251‑1 (MQ=255)                                                                               gcgTGGTGCTCAGTGTGGACTGGACGCTGCTGCTGGTGTTTATGGCGATGTTTATCGACGTCCATTTACTGACCCAGCTTCCAGCGTTGCAAGGCGTGTTGGGTAACGTGAGTCATCTATCTGAACCCGGGTTATGGTTAACGGCAATCGGTTTATCGCAGATGATCAGTAACGTGCCGAGTACCATATTGTTGCTGAACTATGTGCCGCCGTCTTTATTACTGGTATGGGCGGTAAACGTAGGTGGCttt                                                                                                                                                      <  1:298625/251‑1 (MQ=255)                                                                                          aGTGTGGACTGGACGCTGCTGCTGGTGTTTATGGCGATGTTTATCGACGTCCATTTACTGACCCAGCTTCCAGCGTTGCAAGGCGTGTTGGGTAACGTGAGTCATCTATCTGAACCCGGGTTATGGTTAACGGCAATCGGTTTATCGCAGATGATCAGTAACGTGCCGAGTACCATATTGTTGCTGAACTATGTGCCGCCGTCTTTATTACTGGTATGGGCGGTAAACGTAGGTGGCTTTGGGTTATTAcc                                                                                                                                           <  1:203392/251‑1 (MQ=255)                                                                                             gtgGACTGGACGCTGCTGCTGGTGTTTATGGCGATGTTTATCGACGTCCATTTACTGACCCAGCTTCCAGCGTTGCAAGGCGTGTTGGGTAACGTGAGTCATCTATCTGAACCCGGGTTATGGTTAACGGCAATCGGTTTATCGCAGATGATCAGTAACGTGCCGAGTACCATATTGTTGCTGAACTATGTGCCGCCGTCTTTATTACTGGTATGGGCGGTAAACGTAGGTGGCTTTGGGTTATTACCCg                                                                                                                                         <  2:11696/250‑1 (MQ=255)                                                                                             gtgGACTGGACGCTGCTGCTGGTGTTTATGGCGATGTTTATCGACGTCCATTTACTGACCCAGCTTCCAGCGTTGCAAGGCGTGTTGGGTAACGTGAGTCATCTATCTGAACCCGGGTTATGGTTAACGGCAATCGGTTTATCGCAGATGATCAGTAACGTGCCGAGTACCATATTGTTGCTGAACTATGTGCCGCCGTCTTTATTACTGGTATGGGCGGTAAACGTAGGTGGCTTTGGGTTATTACCCg                                                                                                                                         <  2:59775/250‑1 (MQ=255)                                                                                                 actggacGCTGCTGCTGGTGTTTATGGCGATGTTTATCGACGTCCATTTACTGACCCAGCTTCCAGCGTTGCAAGGCGTGTTGGGTAACGTGAGTCATCTATCTGAACCCGGGTTATGGTTAACGGCAATCGGTTTATCGCAGATGATCAGTAACGTGCCGAGTACCATATTGTTGCTGAACTATGTGCCGCCGTCTTTATTACTGGTATGGGCGGTAAACGTAGGTGGCTTTGGGTTATTACCCGGATCg                                                                                                                                    >  2:376863/1‑251 (MQ=255)                                                                                                     gacGCTGCTGCTGGTGTTTATGGCGATGTTTATCGACGTCCATTTACTGACCCAGCTTCCAGCGTTGCAAGGCGTGTTGGGTAACGTGAGTCATCTATCTGAACCCGGGTTATGGTTAACGGCAATCGGTTTATCGCAGATGATCAGTAACGTGCCGAGTACCATATTGTTGCTGAACTATGTGCCGCCGTCTTTATTACTGGTATGGGCGGTAAACGTAGGTGGCTTTGGGTTATTACCCGGATCGCTgg                                                                                                                                <  2:36633/251‑1 (MQ=255)                                                                                                             tgctgGTGTTTATGGCGATGTTTATCGACGTCCATTTACTGACCCAGCTTCCAGCGTTGCAAGGCGTGTTGGGTAACGTGAGTCATCTATCTGAACCCGGGTTATGGTTAACGGCAATCGGTTTATCGCAGATGATCAGTAACGTGCCGAGTACCATATTGTTGCTGAACTATGTGCCGCCGTCTTTATTACTGGTATGGGCGGTAAACGTAGGTGGCTTTGGGTTATTACCCGGATCGCTGGCAAATTTg                                                                                                                        >  2:134766/1‑251 (MQ=255)                                                                                                                        aTGGCGATGTTTATCGACGTCCATTTACTGACCCAGCTTCCAGCGTTGCAAGGCGTGTTGGGTAACGTGAGTCATCTATCTGAACCCGGGTTATGGTTAACGGCAATCGGTTTATCGCAGATGATCAGTAACGTGCCGAGTACCATATTGTTGCTGAACTATGTGCCGCCGTCTTTATTACTGGTATGGGCGGTAAACGTAGGTGGCTTTGGGTTATTACCCGGATCGCTGGCAAATTTGATTGCGCTAc                                                                                                              <  1:162407/250‑1 (MQ=255)                                                                                                                         tGGCGATGTTTATCGACGTCCATTTACTGTCCCAGCTTCCATCGTTGCAAGGCGTGTTTGGTAACGTTAGTCATCTCTCTGAACCCGGGTTATGGTTAACGGCATTCGGTTTATCGCAGATTATCTGTAACGTGCCGATTCCCATATTGTTGCTTAACGATGTTCATCCGTCTTTATTACGGGTATGGGCGGTAAACGTAGGTGGCTTTGGGTTATTTCCCGGATCGCTTCCAAATTTGATTGCGCTACg                                                                                                             <  2:438676/250‑1 (MQ=255)                                                                                                                                   tATCGACGTCCATTTACTGACCCAGCTTCCAGCGTTGCAAGGCGTGTTGGGTAACGTGAGTCATCTATCTGAACCCGGGTTATGGTTAACGGCAATCGGTTTATCGCAGATGATCAGTAACGTGCCGAGTACCATATTGTTGCTGAACTATGTGCCGCCGTCTTTATTACTGGTATGGGCGGTAAACGTAGGTGGCTTTGGGTTATTACCCGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAAc                                                                                                   <  1:16671/250‑1 (MQ=255)                                                                                                                                      cGACGTCCATTTGCTGACCCAGCTTCCAGCGTTGCAAGGCGTGTTGGGTAACGTGAGTCATCTATCTGAACCCGGGTTATGGTTAACGGCAATCGGTTTATCGCAGATGATCATTAACGTGCCGAGTACCATAGTGTTGCTGAACTATGTGCCGCCGTCTTTATTACTTGTATGGGCGGGAAACGTAGTTGGCTTTGGGTTATTACCCGGATCGCTGGCAAATTTGATTGCGCTACGTATGCCGAACGATc                                                                                               <  2:281380/251‑1 (MQ=255)                                                                                                                                          gTCCATTTACTGACCCAGCTTCCAGCGTTGCAAGGCGTGTTGGGTAACGTGAGTCATCTATCTGAACCCGGGTTATGGTTAACGGCAATCGGTTTATCGCAGATGATCAGTAACGTGCCAAGTACCATATTGTTGCTGAACTATGTgccg                                                                                                                                                                                                <  2:312606/150‑1 (MQ=255)                                                                                                                                          gTCCATTTACTGACCCAGCTTCCAGCGTTGCAAGGCGTGTTGGGTAACGTGAGTCATCTATCTGAACCCGGGTTATGGTTAACGGCAATCGGTTTATCGCAGATGATCAGTAACGTGCCAAGTACCATATTGTTGCTGAACTATGTgccg                                                                                                                                                                                                >  1:312606/1‑150 (MQ=255)                                                                                                                                                ttACTGACCCAGCTTCCAGCGTTGCAAGGCGTGTTGGGTAACGTGAGTCATCTATCTGAACCCGGGTTATGGTTAACGGCAATCGGTTTATCGCAGATGATCAGTAACGTGCCGAGTACCATATTGTTGCTGAACTATGTGCCGCCGTCTTTATTACTGGTATGGGCGGTAAACGTAGGTGGCTTTGGGTTATTACCCGGATCGCTGGCAAATTTGATTGCGCTACGTATGGc                                                                                                       <  1:279755/233‑1 (MQ=255)                                                                                                                                                ttACTGACCCAGCTTCCAGCGTTGCAAGGCGTGTTGGGTAACGTGAGTCATCTATCTGAACCCGGGTTATGGTTAACGGCAATCGGTTTATCGCAGATGATCAGTAACGTGCCGAGTACCATATTGTTGCTGAACTATGTGCCGCCGTCTTTATTACTGGTATGGGCGGTAAACGTAGGTGGCTTTGGGTTATTACCCGGATCGCTGGCAAATTTGATTGCGCTACGTATGGc                                                                                                       >  2:279755/1‑233 (MQ=255)                                                                                                                                                 tACTGACCCAGCTTCGAGCGTTGCAAGGCGTGTTGTGTAACGTGAGTCATCTATCTGAACCCGGGTTATGGTTAACGGCAATCTGTTTATCGCAGATGATCAGTAACGTGCCTAGTATCATATTGTTGCTGAACTATGTGCCGCCGTCTTTATTACTGGTATGGGCGTTAAACg                                                                                                                                                                 <  2:197256/174‑1 (MQ=255)                                                                                                                                                 tACTGACCCAGCTTCCAGCGTTGCAAGGCGTGTTGGGTAACGTGAGTCCTCTATCTGAACCCGGGTTATGGTTAACGGCAATCGGTTTATCGCAGATGATCAGTAACGTGCCGCGTACCATATTGTTGCTGAACTATGTGCCGCCGTCTTTATTACTGGTATGGGCGGTAAACg                                                                                                                                                                 >  1:197256/1‑174 (MQ=255)                                                                                                                                                  aCTGACCCAGCTTCCAGCGTGGCAAGGCGTGTTGGGTAACGTGAGTCACCTATCTGAACCCGGGTGATGGTTAACGGCAATCGGTTTAGCGCAGATGATCAGTAACGTGCCGAGTACTAGAGGGTTGCTGAACTATGTGCCGCCGTCTTTATGACTGGTATGGGCGGTAAACGTAGGTGGCTTTGGGTTATTACCCGGATCGCTGGCAAATGTGATTGCTCTACGTAGGGCGAACGATCGCAGCATCtgg                                                                                    <  2:110226/250‑1 (MQ=255)                                                                                                                                                        ccAGCTTCCAGCGTTGCAAGGCGTGTTGGGTAACGTGAGTCATCTATCTGAACCCGGGTTATGGTTAACGGCAATCGGTTTATCGCAGATGATCAGTAACGTGCCGAGTACCATATTGTTGCTGAACTATGTGCCGCCGTCTTTATTACTGGTATGGGCGGTAAACGTAGGTGGCTTTGGGTTATTACCCGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGtt                                                                             <  1:16720/251‑1 (MQ=255)                                                                                                                                                                cAGCGTTGCAAGGCGTGTTGGGTAACGTGAGTCATCTATCTGAACCCGGGTTATGGTTAACGGCAATCGGTTTATCGCAGATGATCAGTAACGTGCCGAGTACCATATTGTTGCTGAACTATGTGCCGCCGTCTTTATTACTGGTATGGGCGGTAAACGTAGGTGGCTTTGGGTTATTACCCGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCAtctc                                                                     <  1:146745/251‑1 (MQ=255)                                                                                                                                                                        cAAGGCGTGTTGGGTAACGTGAGTCATCTATCTGAACCCGGGTTATGGTTAACGGCAATCGGTTTATCGCAGATGATCAGTAACGTGCCGAGTACCATATTGTTGCTGAACTATGTGCCGCCGTCTTTATTACTGGTATGGGCGGTAAACGTAGGTGGCTTTGGGTTATTACCCGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAAt                                                             <  1:376863/251‑1 (MQ=255)                                                                                                                                                                                    ggTAACGTGAGTCATCTATCTGAACCCGGGTTATGGTTAACGGCAATCGGTTTATCGCAGATGATCAGTAACGTGCCGAGTACCATATTGTTGCTGAACTATGTGCCGCCGTCTTTATTACTGGTATGGGCGGTAAACGTAGGTGGCTTTGGGTTATTACCCGGATCGCTGGCAAATTTGATTGCGGTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCGATGCtgtt                                                 <  2:322807/251‑1 (MQ=255)                                                                                                                                                                                    ggTAACGTGAGTCATCTATCTGAACCCGGGTTATGGTTAACGGCAATCGGTTTATCGCAGATGATCAGTAACGTGCCGAGTACCATATTGTTGCTGAACTATGTGCCGCCGTCTTTATTACTGGTATGGGCGGTAAACGTAGGTGGCTTTGGGTTATTACCCGGATCGCTGGCAAATTTGAGTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCGAGGCtgtt                                                 <  2:135388/251‑1 (MQ=255)                                                                                                                                                                                         cGTGAGTCATCTATCTGAACCCGGGTTATGGTTAACGGCAATCGGTTTATCGCAGATGATCAGTAACGTGCCGAGTACCATATTGTTGCTGAACTATGTGCCGCCGTCTTTATTACTGGTATGGGCGGTAAACGTAGGTGGCTTTGGGTTATTACCCGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCGATGCTGTTGTggg                                            <  2:24062/251‑1 (MQ=255)                                                                                                                                                                                                     atctGAACCCGGGTTATGGTTAACGGCAATCGGTTTATCGCAGATGATCAGTAACGTGCCGAGTACCATATTGTTGCTGAACTATGTGCCGCCGTCTTTATTACTGGTATGGGCGGTAAACGTAGGTGGCttt                                                                                                                                                      >  2:252028/1‑133 (MQ=255)                                                                                                                                                                                                     atctGAACCCGGGTTATGGTTAACGGCAATCGGTTTATCGCAGATGATCAGTAACGTGCCGAGTACCATATTGTTGCTGAACTATGTGCCGCCGTCTTTATTACTGGTATGGGCGGTAAACGTAGGTGGCttt                                                                                                                                                      <  1:252028/133‑1 (MQ=255)                                                                                                                                                                                                            cccGGGTTATGGTTAACGGCAATCGGTTTATCGCAGATGATCAGTAACGTGCCGAGTACCATATTGTTGCTGAACTATGTGCCGCCGTCTTTATTACTGGTATGGGCGGTAAACGTAGGTGGCTTTGGGTTATTACCCGGATCGCTGGCAAATTTGATTGCGCTACGTATg                                                                                                         >  2:299125/1‑171 (MQ=255)                                                                                                                                                                                                            cccGGGTTATGGTTAACGGCAATCGGTTTATCGCAGATGATCAGTAACGTGCCGAGTACCATATTGTTGCTGAACTATGTGCCGCCGTCTTTATTACTGGTATGGGCGGTAAACGTAGGTGGCTTTGGGTTATTACCCGGATCGCTGGCAAATTTGATTGCGCTACGTATg                                                                                                         <  1:299125/171‑1 (MQ=255)                                                                                                                                                                                                                   tATGGTTAACGGCAATCGGTTTATCGCAGATGATCAGTAACGTGCCGAGTACCATATTGTTGCTGAACTATGTGCCGCCGTCTTTATTACTGGTATGGGCGGTAAACGTAGGTGGCTTTGGGTTATTGCCCGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATGCAATACCGATGCTGTTGTGGGCGGCGTTTGTGGGATATGTTTTgtt                   <  2:177837/250‑1 (MQ=255)                                                                                                                                                                                                                         tAACGGCAATCGGTTTATCGCAGATGATCAGTAACGTGCCGAGTACCATATTGTTGCTGAACTATGTGCCGCCGTCTTTATTACTGGTATGGGCGGTAAACGTAGGTGGCTTTGGGTTATTACCCGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTtat             <  1:57895/250‑1 (MQ=255)                                                                                                                                                                                                                         tAACGGCAATCGGTTTATCGCAGATGATCAGTAACGTGCCGAGTACCATATTGTTGCTGAACTATGTGCCGCCGTCTTTATTACTGGTATGGGCGGTAAACGTAGGTGGCTTTGGGTTATTACCCGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTtat             >  1:446364/1‑250 (MQ=255)                                                                                                                                                                                                                            cGGCAATCGGTTTATCGCAGATGATCAGTAACGTGCCGAGTACCATATTGTTGCTGAACTATGTGCCGCCGTCTTTATTACTGGTATGGGCGGTAAACGTAGGTGGCTTTGGGTTATTACCCGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTc         <  1:264403/251‑1 (MQ=255)                                                                                                                                                                                                                                  tCGGTTTATCGCAGATGATCAGTAACGTGCCGAGTACCATATTGTTGCTGAACTATGTGCCGCCGTCTTTATTACTGGTATGGGCGGTAAACGTAGGTGGCTTTGGGTTATTACCCGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGcc   >  2:288092/1‑251 (MQ=255)                                                                                                                                                                                                                                   cGGTTTATCGCAGATGATCAGTAACGTGCCGAGTACCATATTGTTGCTGAACTATGTGCCGCCGTCTTTATTACTGGTATGGGCGGTAAACGTAGGTGGCTTTGGGTTATTACCCGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCa  <  1:134766/251‑1 (MQ=255)                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                              TTCTGACGGCGCTGGAGTTCAAACAAGAGCTGTGGGGACTGGTGATTGTGGCGGCAGGCTTTGCGCTGCTGGCACGTCGCGTGGTGCTCAGTGTGGACTGGACGCTGCTGCTGGTGTTTATGGCGATGTTTATCGACGTCCATTTACTGACCCAGCTTCCAGCGTTGCAAGGCGTGTTGGGTAACGTGAGTCATCTATCTGAACCCGGGTTATGGTTAACGGCAATCGGTTTATCGCAGGTGATCAGTAACGTGCCGAGTACCATATTGTTGCTGAACTATGTGCCGCCGTCTTTATTACTGGTATGGGCGGTAAACGTAGGTGGCTTTGGGTTATTACCCGGATCGCTGGCAAATTTGATTGCGCTACGTATGGCGAACGATCGCCGCATCTGGTGGCGTTTCCATCTCTATTCAATACCGATGCTGTTGTGGGCGGCGTTGGTGGGATATGTTTTGTTAGTTATACTCCCGGCCA  >  NC_000913/854284‑854760

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑