Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	857,458	G→A	S499N (AGC→AAC)	ybiT →	putative ATP‑binding protein YbiT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	857,458	0	G	A	100.0%	115.6 / NA	33	S499N (AGC→AAC)	ybiT	putative ATP‑binding protein YbiT
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (16/17);  total (16/17)

CGCAGTATTCTCGGTCGTTTGCTGTTCAGCCAGGACGACATCAAAAAGCCAGCTAAAGTGCTTTCCGGTGGGGAAAAAGGGCGGATGCTGTTTGGTAAGTTAATGATGCAGAAGCCGAACATTCTGATCATGGACGAACCGACCAACCACCTGGATATGGAATCCATTGAGTCGCTGAACATGGCACTGGAACTGTATCAGGGCACGCTGATCTTTGTTTCACACGACCGTGAGTTCGTAAGCTCCCTGGCGACCCGCATTCTGGAAATCACCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTAGGCCGGATAAGGCGTTAAC  >  NC_000913/857217‑857701                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                    cGCAGTATTCTCGGTCGTTTGCTGTTCAGCCAGGACGACATCAAAAAGCCAGCTAAAGTGCTTTCCGGTGGGGAAAAAGGGCGGATGCTGTTTGGTAAGTTAATGATGCAGAAGCCGAACATTCTGATCATGGACGAACCGACCAACCACCTGGATATGGAATCCATTGAGTCGCTGAACATGGCACTGGAACTGTATCAGGGCACGCTGATCTTTGTTTCACACGACCGTGAGTTCGTAAACTCCCTGGc                                                                                                                                                                                                                                            <  1:166142/251‑1 (MQ=255)                     gCTGTTCAGCCAGGACGACATCAAAAAGCCAGCTAAAGTGCTTTCCGGTGGGGAAAAAGGGCGGATGCTGTTTGGTAAGTTAATGATGCAGAAGCCGAACATTCTGATCATGGACGAACCGACCAACCACCTGGATATGGAATCCATTGAGTCGCTGAACATGGCACTGGAACTGTATCAGGGCACGCTGATCTTTGTTTCACACGACCGTGAGTTCGTAAACTCCCTGGCGACCCGCATTCTGGAAATCa                                                                                                                                                                                                                        <  2:332401/251‑1 (MQ=255)                                              aaGCCAGCTAAAGTGCTTTCCGGTGGGGAAAAAGGGCGGATGCTGTTTGGTAAGTTAATTAGGCAGAAGCCGAACAGTCTGATCATGGACGAACCGACCAACCACCTGGATATGGAATCCATTGAGTCGCTGAACATGGCACTGGAACTGTATCAGGGCACGCTGATCTTTGTTTCACACGACCGTGAGTTCGTAAACTCCCTGGCGACCCGCATTCTGGAAATCACCCCGGAACGCGTGATCTACTTTAg                                                                                                                                                                                               <  1:407530/251‑1 (MQ=255)                                                       aaaGTGCTTTCCGGTGGGGAAAAAGGGCGGATGCTGTTTGGTAAGTTAATGATGCAGAAGCCGAACATTCTGATCATGGACGAACCGACCAACCACCTGGATATGGAATCCATTGAGTCGCTGAACATGGCACTGGAACTGTATCAGGGCACGCTGATCTTTGTTTCACACGACCGTGAGTTCGTAAACTCCCTGGCGACCCGCATTCTGGAAATCACCCCGGAACGCGTGATCGGCTTTAGCGGTAAtt                                                                                                                                                                                       <  2:298920/250‑1 (MQ=255)                                                       aaaGTGCTTTCCGGTGGGGAAAAAGGGCGGATGCTGTTTGGTAAGTTAATGATGCAGAAGCCGAACATTCTGATCATGGACGAACCGACCAACCACCTGGATATGGAATCCATTGAGTCGCTGAACATGGCACTGGAACTGTATCAGGGCACGCTGATCTTTGTTTCACACGACCGTGAGTTCGTAAACTCCCTGGCGACCCGCATTCGGGAAATCACCCCGGAACGCGTGATCGACTTTAGCGGTAAt                                                                                                                                                                                        <  1:147569/249‑1 (MQ=255)                                                        aaGTGCTTTCCGGTGGGGAAAAAGGGCGGATGCTGTTTGGTAAGTTAATGATGCAGAAGCCGAACATTCTGATCATGGACGAACCGACCAACCACCTGGATATGGAATCCATTGAGTCGCTGAACATGGCACTGGAACTGTATCAGGGCACGCTGATCTTTGTTTCACACGACCGTGAGTTCGTAAACTCCCTGGCGACCCGCATTCTGGAAATCTCCCCGGAACGCGTGATCTACTTTAGCGGTAATTAc                                                                                                                                                                                     <  2:447838/251‑1 (MQ=255)                                                                 ccGGTGGGGAAAAAGGGCGGATGCTGTTTGGTAAGTTAATGATGCAGAAGCCTAACATTCTGATCATGGACGAACCGACCAACCACCTGGATATGGAATCCATTGAGTCGCTGAACATGGCACTGGAACTGTATCAGGGCACGCTGATCTTTGTTTCACACGACCGTGAGTTCGTAAACTCCCTGGCGACCCGCATTCTGTAAATCACCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTAc                                                                                                                                                                            <  1:273174/251‑1 (MQ=255)                                                                                           tttGGTAAGGTAATGATGCAGAAGCCGAATATTCTGCTCATGGACGAACCGACCAACCACCTGGATATGAAATCCATTGAGTCGCTGAACATGGCACTGGACCTGTATCAGGGCACGCTGATCTTTGTTTCACACGACCGTGAGTTCGTAAACTCCCTGGCGACCAGCATTCTGGAAATCACCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTCGTAAAGGGATCGAGTAAgg                                                                                                                                                  >  2:147363/1‑250 (MQ=255)                                                                                                    ttAATGATGCAGAAGCCGAACATTCTGATCATGGACGAACCGACCAACCACCTGGATATGGAATCCATTGAGTCGCTGAACATGGCACTGGAACTGTATCAGGGCACGCTGATCTTTGTTTCACACGACCGTGAGTTCGTAAACTCCCTGGCGACCCGCATTCTGGAAATCACCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCg                                                                                                                                         >  2:23949/1‑251 (MQ=255)                                                                                                                    cGAACATTCTGATCATGGACGAACCGACCAACCACCTGGATATGGAATCCATTGAGTCGCTGAACATGGCACTGGAACTGTATCAGGGCACGCTGATCTTTGTTTCACACGACCGTGAGTTCGTAAACTCCCTGGCGACCCGCATTCTGGAAATCACCCCGGAACGCGTGAt                                                                                                                                                                                                        <  2:102646/172‑1 (MQ=255)                                                                                                                    cGAACATTCTGATCATGGACGAACCGACCAACCACCTGGATATGGAATCCATTGAGTCGCTGAACATGGCACTGGAACTGTATCAGGGCACGCTGATCTTTGTTTCACACGACCGTGAGTTCGTAAACTCCCTGGCGACCCGCATTCTGGAAATCACCCCGGAACGCGTGAt                                                                                                                                                                                                        >  1:102646/1‑172 (MQ=255)                                                                                                                    cGAACATTCTGATCATGGACGAACCGACCAACCACCTGGATATGGAATCCATTGAGTCGCTGAACATGGCACTGGAACTGTATCAGGGCACGCTGATCTTTGTTTCACACGACCGTGAGTTCGTAAACTCCCTGGCGACCCGCATTCTGGAAATCACCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTc                                                                                                                         >  2:168327/1‑251 (MQ=255)                                                                                                                                tCATGGACGAACCGACCAACCACCTGGATATGGAATCCATTGAGTCGCTGAACATGGCACTGGAACTGTATCAGGGCACGCTGATCTTTGTTTCACACGACCGTGAGTTCGTAAACTCCCTGGCGACCCGCATTCTGGAAATCACCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTACAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGAcggcg                                                                                                             >  1:415716/1‑251 (MQ=255)                                                                                                                                  aTGGACGAACCGACCAACCACCTGGATATGGAATCCATTGAGTCGCTGAACATGGCACTGGAACTGTATCAGGGCACGCTGATCTTTGTTTCACACGACCGTGAGTTCGTAAACTCCCTGGCGACCCGCATTCTGGAAATCACCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGGGACggcggc                                                                                                           >  1:85661/1‑251 (MQ=255)                                                                                                                                    ggACGAACCGCCCAACCCACTGGATATGGAATCCATTGAGTCGCTGAACATGGCACTGGAACTGTATCAGGGCACGCTGATCTTTGTTTCACACGACCGTGAGTTCGTAAACTCCCTGGCGACCCGCATTCTGGAAATCACCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCtg                                                                                                         <  2:242842/251‑1 (MQ=255)                                                                                                                                        gAACCGACCAACCACCTGGATATGGAATCCATTGAGTCGCTGAACATGGCACTGGAACTGTATCAGGGCACGCTGATCTTTGTTTCACACGACCGTGAGTTCGTAAACTCCCTGGCGACCCGCATTCTGGAAATCACCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCtgatg                                                                                                      >  2:232172/1‑250 (MQ=255)                                                                                                                                             gACCAACCACCTGGATATGGAATCCATTGAGTCGCTGAACATGGCACTGGAACTGTATCAGGGCACGCTGATCTTTGTTTCACACGACCGTGAGTTCGTAAACTCCCTGGCGACCCGCATTCTGGAAATCACCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAAt                                                                                                >  1:348082/1‑251 (MQ=255)                                                                                                                                                             atGGAATCCATTGAGTCGCTGAACATGGCACTGGAACTGTATCAGGGCACGCTGATCTTTGTTTCACACGACCGTGAGTTCGTAAACTCCCTGGCGACCCGCATTCTGGAAATCACCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATg                                                                                >  1:90496/1‑251 (MQ=255)                                                                                                                                                               ggAATCCATTGAGTCGCTGAACATGGCACTGGAACTGTATCAGGGCACGCTGATCTTTGTTTCACACGACCGTGAGTTCGTAAACTCCCTGGCGACCCGCATTCTGGAAATCACCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATgcg                                                                              >  1:71009/1‑251 (MQ=255)                                                                                                                                                                          aGTCGCTGAACATGGCACTGGAACTGTATCAGGGCACGCTGATCTTTGTTTCACACGACCGTGAGTTCGTAAACTCCCTGGCGACCCGCATTCTGGAAATCACCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCCTATc                                                                   >  2:224644/1‑251 (MQ=255)                                                                                                                                                                           gTCGCTGAACATGGCACTGGAACTGTATCAGGGCACGCTGATCTTTGTTTCACACGACCGTGAGTTCGTAAACTCCCTGGCGACCCGCATTCTGGAAATCACCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCa                                                                  <  1:412808/251‑1 (MQ=255)                                                                                                                                                                                  aaCATGGCACTGGAACTGTATCAGGGCACGCTGATCTTTGTTTCACACGACCGTGAGTTCGTAAACTCCCTGGCGACCCGCATTCTGGAAATCACCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATTCTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTAc                                                           <  2:90496/251‑1 (MQ=255)                                                                                                                                                                                     aTGGCACTGGAACTGTATCAGGGCACGCTGATCTTTGTTTCACACGACCGTGAGTTCGTAAACTCCCTGGCGACCCGCATTCTGGAAATCACCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCCGGCCTACGcc                                                        >  2:139549/1‑250 (MQ=255)                                                                                                                                                                                     aTGGCACTGGAACTGTATCAGGGCACGCTGATCTTTGTTTCACACGACCGTGAGTTCGTAAACTCCCTGGCGACCCGCATTCTGGAAATCACCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGc                                                         >  2:236712/1‑250 (MQ=255)                                                                                                                                                                                       ggCACTGGAACTGTATCAGGGCACGCTGATCTTTGTTTCACACGACCGTGAGTTCGTAAACTCCCTGGCGACCCGCATTCTGGAAATCACCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTTCCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGt                                                      <  2:85661/251‑1 (MQ=255)                                                                                                                                                                                       ggCACTGGAACTGTATCAGGGCACGCTGATCTTTGTTTCACACGACCGTGAGTTCGTAAACTCCCTGGCGACCCGCATTCTGGAAATCACCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGt                                                      <  1:232172/251‑1 (MQ=255)                                                                                                                                                                                                aCTGTATCAGGGCACGCTGATCTTTGTTTCACACGACCGTGAGTTCGTAAACTCCCTGGCGACCCGCATTCTGGAAATCACCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAat                                             <  1:282052/251‑1 (MQ=255)                                                                                                                                                                                                            cACGCTGATCTTTGTTTCACACGACCGTGAGTTCGTAAACTCCCTGGCGACCCGCATTCTGGAAATCACCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACATGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTg                                 >  1:88598/1‑251 (MQ=255)                                                                                                                                                                                                                         gTTTCACACGACCGTGAGTTCGTAAACTCCCTGGCGACCCGCATTCTGGAAATCACCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTa                     <  1:232767/250‑1 (MQ=255)                                                                                                                                                                                                                                    ccGTGAGTTCGTAAACTCCCTGGCGACCCGCATTCTGGAAATCACCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTAGGCCGGATAAgg         <  1:317996/251‑1 (MQ=255)                                                                                                                                                                                                                                        gAGTTCGTAAACTCCCTGGCGACCCGCATTCTGGAAATCACCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAgg                             >  2:263837/1‑227 (MQ=255)                                                                                                                                                                                                                                        gAGTTCGTAAACTCCCTGGCGACCCGCATTCTGGAAATCACCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAgg                             <  1:263837/227‑1 (MQ=255)                                                                                                                                                                                                                                           ttCGTAAACTCCCTGGCGACCCGCATTCTGGAAATCACCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTAGGCCGGATAAGGCGTTAAc  >  2:306664/1‑251 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                    CGCAGTATTCTCGGTCGTTTGCTGTTCAGCCAGGACGACATCAAAAAGCCAGCTAAAGTGCTTTCCGGTGGGGAAAAAGGGCGGATGCTGTTTGGTAAGTTAATGATGCAGAAGCCGAACATTCTGATCATGGACGAACCGACCAACCACCTGGATATGGAATCCATTGAGTCGCTGAACATGGCACTGGAACTGTATCAGGGCACGCTGATCTTTGTTTCACACGACCGTGAGTTCGTAAGCTCCCTGGCGACCCGCATTCTGGAAATCACCCCGGAACGCGTGATCGACTTTAGCGGTAATTACGAAGATTACCTGCGTAGTAAAGGGATCGAGTAAGTGATTATCCGTCACCCCGATACTGTCGGGGTGACGGCGGCTGATGACAAATGCAAAATAGCCTGATGCGCTACGCTTATCAGGCCTACGCAGTACCTGCAATATATTGAATCTGCAGGACTTTGTAGGCCGGATAAGGCGTTAAC  >  NC_000913/857217‑857701

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑