Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,085,802	G→A	V271I (GTC→ATC)	phoH →	ATP‑binding protein PhoH

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,085,802	0	G	A	100.0%	144.6 / NA	46	V271I (GTC→ATC)	phoH	ATP‑binding protein PhoH
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (22/24);  total (22/24)

GATACATAAGGATGTCGACAGGATTATCGTCACCCGTCCAGTTCTGCAAGCCGATGAAGATCTTGGCTTCTTACCTGGAGATATCGCAGAAAAGTTTGCTCCCTATTTTCGCCCGGTCTACGACGTGCTGGTCCGGCGCTTAGGGGCTTCCTTTATGCAGTACTGCCTGCGACCGGAAATAGGGAAGGTGGAAATTGCGCCGTTCGCCTATATGCGTGGACGTACCTTTGAAAATGCAGTCGTCATTCTTGACGAGGCGCAGAATGTGACTGCCGCGCAAATGAAAATGTTTTTAACCCGCCTCGGGGAGAACGTGACGGTTATCGTCAACGGTGATATCACGCAATGCGATTTGCCTCGCGGCGTGTGCTCCGGATTAAGTGACGCGCTGGAACGTTTCGAAGAAGATGAAATGGTTGGGATCGTCCGGTTCGGTAAAGAGGACTGCGTACGTTCGGCACTTTGCCAACGTACGCTGC  >  NC_000913/1085564‑1086042                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                 gATACATAAGGATGTCGACAGGATTATCGTCACCCGTCCAGTTCTGCAAGCCGATGAAGATCTTGGCTTCTTACCTGGAGATATCGCAGAAAAGTTTGCTCCCTATTTTCGCCCGGTCTACGACGTGCTGGTCCGGCGCTTAGGGGCTTCCTTTATGCAGTACTGCCTGCGACCGGAAATAGGGAAGGTGGAAATTGCGCCGTTCGCCTATATGCGTGGACGTACCTTTGAAAATGCAATCGTCATTCTTg                                                                                                                                                                                                                                      >  1:226/1‑251 (MQ=255) gATACATAAGGATGTAGACAGGATTATCGTCACCCGTCCAGTTCTGCAAGCCGATGAAGATCTTGGCTTCTTACCTGGAGATATCGCAGAAAAGTTTGCTCCCTATTTTCGCCCGGTCTACGACGTGCTGGTCCGGCGCTTAGGGGCTTCCTTTATGCAGTACTGCCTGCGACCGGAAATAGGGAAGGTGGAAATTGCGCCGTTCGCCTATATGCGTGGACGTACCTTTGAAAATGCAATCGTCATTCTTg                                                                                                                                                                                                                                      >  1:327475/1‑251 (MQ=255)   tACATAAGGATGTCGACAGGATTATCGTCACCCGTCCAGTTCTGCAAGCCGATGAAGATCTTGGCTTCTTACCTGGAGATATCGCAGAAAAGTTTGCTCCCTATTTTCGCCCGGTCTACGACGTGCTGGTCCGGCGCTTAGGGGCTTCCTTTATGCAGTACTGCCTGCGACCGGAAATAGGGAAGGTGGAAATTGCGCCGTTCGCCTATATGCGTGGACGTACCTTTGAAAATGCAATCGTCATTCTTGAc                                                                                                                                                                                                                                    >  1:124814/1‑251 (MQ=255)           gATGTCGACAGGATTATCGTCACCCGTCCAGTTCTGCAAGACGATGAAGATCTTGGCTTCTTACCTGGAGATATCGCAGAAAAGTTTGCTCCCTATTTTCGCCCGGTCTACGACGTGCTGGTCCGGCGCTTAGGGGCTTCCTTTATGCAGTACTGCCTGCGACCGGAAATAGGGAAGGTGGAAATTGCGCCGTTCGCCTATATGCGTGGACGTACCTTTGAACATGCAATCGTCATTCTTGACGAGgcgcc                                                                                                                                                                                                                            >  2:430618/1‑250 (MQ=255)             tGTCGACAGGATTAGCGTCACCCGTCCAGTTCTGCAAGCCGATGAAGATCTTGGCTTCTTACCTGGAGATATCGCAGAAAAGTTTGCTCCCTATTTTCGCCCGGTCTACGACGTGCTGGTCCGGCGCTTAGGGGCTTCCTTTATGCAGTACTGCCTGCGACCGGAAATAGGGAAGGTGGAAATTGCGCCGTTCGCCTATATGCGTGGACGTACCTTTGAAAATGCAATCGTCATTCTTGACGAGgcgcgg                                                                                                                                                                                                                           >  2:277237/1‑248 (MQ=255)                cGACAGGATTATCGTCCCCCGTCCAGTTCTGCAAGCCGATGAAGATCTTGGCTTCTTACCTGGAGATATCGCAGAAAAGTTTGCTCCCTATTTTCGCCCGGTCTACGACGTGCTGGTCCGGCGCTTAGGGGCTTCCTTTATGCAGTACTGCCTGCGACCGGAAATAGGGAAGGTGGAAATTGCGCCGTTCGCCTATATGCGTGGACGTACCTTTGAAAATGCAATCGTCATTCTTGACGAGGCGCAGAAt                                                                                                                                                                                                                        <  2:66225/250‑1 (MQ=255)                  aCAGGATTATCGTCACCCGTCCAGTTCTGCAAGCCGATGAAGATCTTGGCTTCTTACCTGGAGATATCGCAGAAAAGTTTGCTCCCTATTTTCGCCCGGTCTACGACGTGCTGGTCCGGCGCTTAGGGGCTTCCTTTATGCAGTACTGCCTGCGACCGGAAATAGGGAAGGTGGAAATTGCGCCGTTCGCCTATATGCGTTGACGTGCCTTTGAAAATGCAATCGTCATTCTTGACGAGGCGCAGAAtgtg                                                                                                                                                                                                                     <  1:430618/251‑1 (MQ=255)                         tANCGTCACCCGTCCAGTTCTGCAAGCCGATGAAGATCTTGGCTTCTTACCTGGAGATATCGCAGAAAAGTTTGCTCCCTATTTTCGCCCGGTCTACGACGTGCTGGTCCGGCGCTTAGGGGCTTCCTTTATGCAGTACTGCCTGCGACCGGAAATAGGGAAGGGGGAAATTGCGCCGTTCGCCTATATGCGTGGACGTACCTTTGAAAATGCAATCGTCATTCTTGACGAGGCGCAGAATGTGACTGcc                                                                                                                                                                                                               <  2:411512/250‑1 (MQ=255)                              tCACCCGTCCAGTTCTGCAAGCCGATGAAGATCTTGGCTTCTTACCTGGAGATATCGCAGAAAAGTTTGCTCCCTATTTTCGCCCGGTCTACGACGTGCTGGTCCGGCGCTTAGGGGCTTCCTTTATGCAGTACTGCCTGCGACCGGAAATAGGGAAGGTGGAAATTGCGCCGTTCGCCTATATGCGTGGACGTACCTTTGAAAATGCAATCGTCATTCTTGACGAGGCGCAGAATGTGACGGCCGCGCa                                                                                                                                                                                                          >  1:404154/1‑250 (MQ=255)                                aCCCGTCCAGTTCGGCAAGCCTATGAATATCTTGGCTTCTTACCTGGAGAGATCGCAGAAAAGTTTGCTCCCTAATGTCGCCCGGTCTACGACGTGCTGGTCCGGCGCTTAGGGGCTTCCTTTATGCAGTACTGCCTGCGACCCGAAATAGGGAAGTTGGAAATTGCGCCGTTCGCCTATATGCGTGGACGTTCCTTTGACAATGCAATCGTCATTCTTGACGAGGCGCAGAATGTGACTGCCGCGCAAAt                                                                                                                                                                                                       <  2:296241/251‑1 (MQ=255)                                      ccatttCTGCAAGCCGATGAAGATCTTGGCTTTTTACCTGGAGATATCGCAGAAAAGTTTGCTCCCTATTTTCGCCCGGTCTACGACGTGCTGGTCCGGCGCTTAGGGGCTTTCTTTATGCAGTACTGCCTGCGTCCGGAAATAGGGAAGGTGGAAATTGCGCCGTTCGCCTATATGCGTGGACGTGCCTTTGAAAATGCAATCGTCATTCTTTACGAGGCGCAGAATGTGACTGCCGCGCAAATGAAAAt                                                                                                                                                                                                 <  1:270316/247‑1 (MQ=255)                                      ccAGTTCTGCAAGCCGATGAAGATCTTGGCTTCTTACCTGGAGATATCGCAGAAAAGTTTGCTCCCTATTTTCGCCCGGTCTACGACGTGCTGGTCCGGCGCTTAGGGGCTTCCTTTATGCAGTACTGCCTGCGACCGGAAATAGGGAAGGTGGAAATTGCGCCGTTCGCCTATATGCGTGGACGTACCTTTGAAAATGCAATCGTCATTCTTGACGAGGCGCAGAATGTGACTGCCGCGCAAATGAAAAt                                                                                                                                                                                                 <  1:237211/251‑1 (MQ=255)                                        aGTTCTGCAAGCCGATGAAGATCTTGGCTTCTTACCTGGAGATATCGCAGAAAAGTTTGCTCCCTATTTTCGCCCGGTCTACGACGTGCTGGTCCGGCGCTTAGGGGCTTCCTTTATGCAGTACTGCCTGCGACCGGAAATAGGGAAGGTGGAAATTGCGCCGTTCGCCTATATGCGTGGACGTACCTTTGAAAATGCAATCGTCATTCTTGACGAGGCGCAGAATGTGACTGCCGCGCAAATGAAAATGt                                                                                                                                                                                               >  1:163011/1‑251 (MQ=255)                                                 aGCCGATGAAGATCTTGGCTTCTTACCTGGAGATATCGCAGAAAAGTTTGCTCCCTATTTTCGCCCGGTCTACGACGTGCTGGTCCGGCGCTTAGGGGCTTCCTTTATGCAGTACTGCCTGCGACCGGAAATAGGGAAGGTGGAAATTGCGCCGTTCGCCTATATGCGTGGACGTACCTTTGAAAATGCAATCGTCATTCTTGACGAGGCGCAGAATGTGACTGCCGCGCAAATGAAAATGTTTTTAAccc                                                                                                                                                                                      <  1:180227/251‑1 (MQ=255)                                                  gCCGATGAAGATCTTGGCTTCTTACCTGGAGATATCGCAGAAAAGTTTGCTCCCTATTTTCGCCCGGTCTACGACGTGCTAGTCCGGCGCTTAGGGGCTTCCTTTATGCAGTACTGCCTGCGACCGGAAATAGGGAAGGTGGAAATTGCGCCGTTCGCCTATATGCGTGGACGTACCTTTGAAAATGCAATCGTCATTCTTGACGAGGCGCAGAATGTGACTGCCGCGCAAATGAAAATGTTTTTAACCCg                                                                                                                                                                                     <  2:82147/251‑1 (MQ=255)                                                    cGATGAAGATCTTGGCTTCTTACCTGGAGATATCGCAGAAAAGTTTGCTCCCTATTTTCGCCCGGTCTACGACGTGCTGGGCCGGCGCTTAGGGGCTTCCTTTATGCAGTACTGCCTGCGACCGGAAATAGGGAAGGTGGAAATTGCGCCGTGCGCCTATATGCGTGGACGTACCGTTGAAAATGCAATCGGCATTTTTGTCGAGGCGCAGAATGTGACTGCCGCGCAAATGAAAATGTTTTTAACCCGcc                                                                                                                                                                                   <  1:291749/251‑1 (MQ=255)                                                     gATGAAGATCTTGGCTTCTTACCTGGAGATATCGCAGAAAAGGTTGCTCCCTATTTTCGCCCGGTCTACGACGTGCTGGTCCGGCGCTTAGGGGCTTCCTTTATGCAGTACTGCCTGCGACCGGAAATAGGGAAGGTGGAAATTGCGCCGTTCGCCTATATGCGTGGACGTACCTTTGAAAATGCAATCGTCATTCTTGACGAGGCGCAGAATGTGACTGCCGCGCAAATTAAAATGTTTTTAACCCGCCt                                                                                                                                                                                  <  1:369549/251‑1 (MQ=255)                                                        gAAGATCTTGGCTTCTTACCTGGAGATATCGCAGAAAAGTTTGCTCCCTATTTTCGCCCGGTCTACGACGTGCTGGTCCGGCGCTTAGGGGCTTCCTTTATGCAGTACTGCCTGCGACCGGAAATAGGGAAGGTGGAAATTGCGCCGTTCGCCTATATGCGTGGACGTACCTTTGAAAATGCAATCGTCATTCTTGACGAGGCGCAGAATGTGACTGCCGCGCAAATGAAAATGTTTTTAACCCGCCTCgg                                                                                                                                                                               >  1:98487/1‑251 (MQ=255)                                                        gAAGATCTTGGCTTCTTACCTGGAGATATCGAAGAAAAGTTTGCTCCCTATTTTCGCCCGGTCTACGACGTGCTGGTCCGGCGCTTAGGGGCTTCTTTTAGGCAGTACTGCCGGCGACCGGAAATAGGGAAGGGGGAAATTGCGCCGTTCGCCTATAGGCGTGGACGTACCTTTGAAAATGCAATCGTCATTCTTGACGAGGCGCAGAATGTGACTGCCGCGCAAATGAAAATGTTTTTAACCCGCCTCgg                                                                                                                                                                               <  1:68261/251‑1 (MQ=255)                                                                    ttcttACCTGGAAATATAGCAGAAAAGTTTGCTCCCTATTTTCGCCCGGTCTACGACGTGCTGGTCCGGCGCTTAGGGGCTTCCTTTAGGCAGTACTGCCTGCGACCGGAAATAGGGAAGGTGGAAATTGCGCCGTTCGCCTATATGCGTGGACGTACCTTTGAAAATGCAATCGTCATTCTTGACGAGGCGCAGAATGTGACTGCCGCGCAAATGAAAATGTTTTTAACCCGCCTCGGGGAGAACGTGa                                                                                                                                                                    <  1:365860/250‑1 (MQ=255)                                                                                 atatCGCAGAAAAGTTGGCTCCCTTTTTTCGCCCGGTCTACGACGTGTTGGTCCGGCGCTTAGGGGCTTCCTTTATGCGGTACTGCCTGCGACCGGAAAGAGGGAAGGTGGAAATTGCGCCGTTCGCCTATATGCGTGGACTTCCCTTGGAAAATGCAATCGTCATTCTTGACGAGGCGCAGAATGGGACTGCCGCGCAAATGAAAATGTTTTTAACCCGCCTCGGGGAGAACGTGACGGTTATCGTCAAc                                                                                                                                                      <  2:388276/251‑1 (MQ=255)                                                                                 atagcGCAAAAAAGTTTGCTCCCTATTTTCGCCCGGTCTCCGACGGGCTGGTCCGGCGCTTAGGGGCTTCCTTTAGGCAGTACTGCCTGCGACCGGAAAAAGGGAAGGTGGAAATTGCGCCTTTCGCCTATATGCGGGGACGTACCTTTGAAAATGCAATCGTCATTCTTGACGAGGCGCAAAATGGGACTGCCGCGCAAATAAAAAGGTTTTTAACCCGCCTCGGGGAGAACGTGACGGTTATCGTCAAc                                                                                                                                                      <  2:155410/247‑1 (MQ=255)                                                                                                ttGCTCCCTATTTTCGCCCGGTCTACGACGTGCTGGTCCGGCGCTTAGGGGCTTCCTTTATGCAGTACTGCCTGCGACCGGAAATAGGGAAGGTGGAAATTGCGCCGTTCGCCTATATGCGTGGACGTACCTTTGAAAATGCAATCGTCATTCTTGACGAGGCGCAGAATGTGACTGCCGCGCAAATGAAAATGTTTTTAACCCGCCTCGGGGAGAACGTGACGGTTATCGTCAACGGTGATATCACGCa                                                                                                                                        >  1:162448/1‑250 (MQ=255)                                                                                                  gCTCCCTATTTCCGCCCGGTCAGCGACGTGCTGGTCCGGCGTTTGGGGGCTTCCTTTAGGCAGTACTGCGTGCGACCGGAAATAGGGAAGGTGGAAATTGCGCTTTTCGCCTATATGCGTGGACGTCCCTTTGAAAATGCAATCGTAATTCTTGACGAGGCGCAGAAGGTGACTGCCGCGTAAATGAAAATGTTTTTAAGCCGCCTCGGGGATAACGTGACGGGGATCGTCAACGGTGATATcgggcaat                                                                                                                                      <  2:59737/250‑8 (MQ=255)                                                                                                        tATTTTCGCCCGGTCTACGACCTGGTGGTCCGGAGCTTAGGGGCATCCTTTACGCAGTACTGCCTGCGACCGGAAATAGGGAAGATGGCAATTGCGCCGTTCGCATATATGCGTGGACGTACCTTTGAAAATGCAATCGTCATTCTTGACGAGGCGCAGAATGTGACTGCCGCGCAAATGACAATGTTTTTAACCCGCCTCGGGGAGAACGTGACGGTTATCGTCAACGGTGATATCACGCAATGCGAttt                                                                                                                               >  2:456178/1‑251 (MQ=255)                                                                                                                      cTACGACGTGCTGGTCCGGCGCTTAGGGGCTTCCTTTATGCAGTACTGCCTGCGACCGGAAATAGGGAAGGTGGAAATTGCGCCGTTCGCCTATATGCGTGGACGTACCTTTGAAAATGCAATCGTCATTCTTGACGAGGCGCAGAATGTGACTGCCGCGCAAATGAAAATGTTTTTAACCCGCCTCGGGGAGAACGTGACGGTTATCGTCAACGGTGATATCACGCAATGCGATTTGCCTCGCGGCgtgt                                                                                                                 >  1:37523/1‑251 (MQ=255)                                                                                                                             gTGCTGGTCCGGCGCTTATGTGTTTCCTTTATGCAGTACTGCCTGCTACCGGAAATAGGGAATGTGGAAATTGCGCCTTTCGCCTATATGCGTGGAGGTCCCTTTGAAAATCCAATCGTCATTCTTGACGAGGCGCAGAATGGGACTGCCGCGCAAATTAAAATTTTTTTACCCCGCCTCGGGGAGAACGTGACGGTTATCGTCAACGGTGATATCCCGCAATGCGATTTGCCTCGCGGCGTGTGCTCCgg                                                                                                          <  2:208220/251‑1 (MQ=255)                                                                                                                                      cGGCGCTTAGGGGCTTCCTTTATGCAGTACTGCCTGCGACCGGAAATAGGGAAGGTGGAAATTGCGCCGTTCGCCTATATGCGTGGACGTACCTTTGAAAATGCAATCGTCATTCTTGACGAGGCGCAGAATGTGACTGCCGCGCAAATGAAAATGTTTTTAACCCGCCTCGGGGAGAACGTGACGGTTATCGTCAACGGTGATATCACGCAATGCGATTTGCCTCGCGGCGTGTGCTCCGGATTAAGTg                                                                                                  >  1:267683/1‑250 (MQ=255)                                                                                                                                       ggCGCTTAGGGGCTTCCTTTATGCAGTACTGCCTGCGACCGGAAATAGGGAAGGTGGAAATTGCGCCGTTCGCCTATATGCGTGGACGTACCTTTGAAAATGCAATCGTCATTCTTGACGAGGCGCAGAATGTGACTGCCGCGCAAATGAAAATGTTTTTAACCCGCCTCGGGGAGAACGTGACGGTTATCGTCAACGGTGATATCACGCAATGCGATTTGCCTCGCGGCGTGTGCTCCGGATTAAGTGAc                                                                                                >  1:344448/1‑251 (MQ=255)                                                                                                                                         cgcTTAGGGGTTTCCTTTATGCAGTACTGCCTGCGCCCGGAAATAGGGAAGGTGGAATTTGCGCCGTTCGCCTATATGCGTGGACGTACCTTTGAAAATGCAATCGTCATTCTGGACGAGGCGCAGAAGGTGACTGCCGCGCAAATGAAAATGTTTTTAACCCGCCTCGGGGAGAACGTGACGGTTATCGTCAACGGTGATATCACGCAATGCGATTTGCCTCGCGGCGTGTGCTCCGGATTAAGTGAcgc                                                                                              <  1:450038/251‑1 (MQ=255)                                                                                                                                         cgcTTAGGGGCTTCCTTTATGCAGTACTGCCTGCGACCGGAAATAGGGAAGGTGGAAATTGCGCCGTTCGCCTATATGCGTGGACGTACCTTTGAAAATGCAATCGTCATTCTTGACGAGGCGCAGAATGTGACTGCCGCGCAAATGAAAATGTTTTTAACCCGCCTCGGGGAGAACGTGACGGTTATCGTCAACGGTGATATCACGCAATGCGATTTGCCTCGCGGCGTGTGCTCCGGATTAAGTGAcgc                                                                                              >  1:364327/1‑251 (MQ=255)                                                                                                                                           cTTAGGGGCTTCCTTTATGCAGAACTGCCTGCGACCGGAAATAGGGAAGGTGGAAATTGCGCCGTTCGCCTATATGCGTGGACGTACCTTTGAAAATGCAATCGTCATTCTTGACGAGGCGCAGAATGTGACTGCCGCGCAAATGAAAATGTTTTTAACCCGCCTCGGGGAGAACGTGACGGTTATCGTCAACGGTGATATCACGCAATGCGATTTGCCTCGCGGCGTGTGCTCCGGCTTAAGTGACgcgc                                                                                            >  2:89698/1‑251 (MQ=255)                                                                                                                                                     tCCTTTATGCAGTACTGCCTGCGACCGGAAATAGGGAAGGTGGAAATTGCGCCGTTCGCCTATATGCGTGGACGTACCTTTGAAAATGCAATCGTCATTCTTGACGAGGCGCAGAATGTGACTGCCGCGCAAATGAAAATGTTTTTAACCCGCCTCGGGGAGAACGTGACGGTTATCGTCAACGGTGATATCACGCAATGCGATTTGCCTCGCGGCGTGTGCTCCGGATTAAGTGACGCGCTGGAACGttt                                                                                  >  1:14400/1‑251 (MQ=255)                                                                                                                                                              cAGTACTGCCTGAGAACGGAAATATGAAAGGTGGAAATTGCGCCGTTCGCCTTTATTCGTGGACGTACTTTTAAAAAGTCAAGCGTCATTTTTGACGAGGCGCAGAAGGTGACGGCCGCGCAAATGAAAAGTTTTTTACCCCGCCTCGGGGAGAACGTGACGGTTATCGTCAACGGTGATATCACGCAATGCGATTTGCCTCGCGGCGTGTGCTCCGGATTAAGTGACGCGCTGGAACGTTTCGAagaaga                                                                         <  2:327475/251‑1 (MQ=255)                                                                                                                                                                gTACTGCCTGCGCCCGGAAAGAGGGAAGGTGGAAATTGCGCCGTTCGCCTATATGCGTGGACGTACCTTTGAAAATGAAATCGTCATTCTTGACGAGGCGCAGAATGTGACTGCCGCGAAAATGAAAATGTTTTTAACCCGCCTCGGGGAGAACGTGACGGTTATCGTCAACGGTGATATCACGCAATGCGATTTGCCTCGCGGCGTGTGCTCCGGATTAAGTGACGCGCTGGAACGTTTCGAAGAAGATg                                                                       <  2:124814/251‑1 (MQ=255)                                                                                                                                                                        tgcGACCGGAAATAGGGAAGGTGGAAATTGCGCCGTTCGCCTATATGCGTGGACGTACCTTTGAAAATGCAATCGTCATTCTTGACGAGGCGCAGAATGTGACTGCCGCGCAAATGAAAATGTTTTTAACCCGCCTCGGGGAGAACGTGACGGTTATCGTCAACGGTGATATCACGCAATGCGATTTGCCTCGCGGCGTGTGCTCCGGATTAAGTGACGCGCTGGAACGTTTCGAAGAAGATGAAATGGtt                                                               >  1:368273/1‑251 (MQ=255)                                                                                                                                                                        ggcGACCGGAAATAGGGAAGGTGGAAATTGCGCCGTTCGCCTATATGCGTGGACGTACCTTTGAAAATGCAATCGTCATTCTTGACGAGGCGCAGAATGTGACTGCCGCGCAAATAAAAATGTTTTTAACCCGCCTCGGGGAGAACGTGACGGTTATCGTCAACGGTGATATCACGCAATGCGATTTGCCTCGCGGCGTGTGCTCCGGATTAAGTGACGCGCTGGAACGTTTCGAAGAAGATGAAATGGtt                                                               <  2:14400/250‑1 (MQ=255)                                                                                                                                                                           gACCTGAAATAGGGAAGGTGGAAATTGCGCCGTTCGCCTATAGGCGTGGACGTACCTTTGAAAATTCAATCGTCATTCTTGACTGGGCGCAGAATGTGACTGCCGCGCAAATGAAAATTTTTTTAACCCGCCTCGGGGAGA‑CGTGACGGTTATCGTCAACGGTGATATCACGCAATGCGATTTGCCTCGCGGCGTGTGCTCCGGATTAAGTGACGCGCTGGAACGTTTCGAAGAAGATGAAATGGTTGGGa                                                           <  1:36595/251‑1 (MQ=255)                                                                                                                                                                                   aTAGGGAAGGTGAAAATTGCGCCGTTCGCCTATATGCGTGGACGTCCCTTTGAAAATGCAATCGTCATTCTTGCCGAGGCGCAGAATGTGACTGCCGCGCAAATGAAAATGTTTTTAACCCGCCTCGGGGAGAACGTGACGGTTATCGTCAACGGTGATATCACGCAATGCGATTTGCCTCGCGGCGTGTGCTCCGGATTAAGTGACGCGCTGGAACGTTTCGAAGAAGATGAAATGGTTGGGATCGTCCg                                                    <  1:148713/251‑1 (MQ=255)                                                                                                                                                                                          aGGTGGAAATTGCGCCGTTCGCCTATATGCGTGGACGTACCTTTGAAAATGCAATCGTCATTCTTGACGAGGCGCAGAATGTGACTGCCGCGCAAATGAAAATGTTTTTAACCCGCCTCGGGGAGAACGTGACGGTTATCGTCAACGGTGATATCACGCAATGCGATTTGCCTCGCGGCGTGTGCTCCGGATTAAGTGACGCGCTGGAACGTTTCGAAGAAGATGAAATGGTTGGGATCGTCCGGTTCGGt                                             >  2:354795/1‑251 (MQ=255)                                                                                                                                                                                                               ccTATATGCGTGGACGTACCTTTGAAAATGCAATCGTCATTCTTGACGAGGCGCAGAATGTGACTGCCGCGCAAATGAAAATGTTTTTAACCCGCCTCGGGGAGAACGTGACGGTTATCGTCAACGGTGATATCACGCAATGCGATTTGCCTCGCGGCGTGTGCTCCGGATTAAGTGACGCGCTGGAACGTTTCGAAGAAGATGAAATGGTTGGGATCGTCCGGTTCGGTAAAGAGGACTGCGTACGTTCg                        >  2:220331/1‑251 (MQ=255)                                                                                                                                                                                                                  atatGCGTGGACGTACCTTTGAAAATGAAATCGTAATTCTTGACGAGGCGCAGAAGGTGACTGCCGCGCAAATGAAAATGTTTTTAACCCGCCTCGGGGAGAACGTGACGGTTATCGTCAACGGTGATATCACGCAATGCGATTTGCCTCGCGGCGTGTGCTCCGGATTAAGTGACGCGCTGGAACGTTTCGAAGAAGATGAAATGGTTGGGATCGTCCGGTTCGGTAAAGAGGACTGCGTACGTTCGGCa                     <  2:163011/251‑1 (MQ=255)                                                                                                                                                                                                                      gCGTGGGCGTACCTTTGAAAATGCAATCGTCATTCTTGACGAGGCGCAGAATGTGACTGCCGCGCAAATGAAAATGTTTTTAACCCGCCTCGGGGAGAACGTGACGGTTATCGTCAACGGTGATATCACGCAATGCGATTTGCCTCGCGGCGTGTGCTCCGGATTAAGTGACGCGCTGGAACGTTTCGAAGAAGATGAAATGGTTGGGATCGTCCGGTTCGGTAAAGAGGACTGCGTACGTTCGGCACtt                  <  1:124626/250‑1 (MQ=255)                                                                                                                                                                                                                        gTGGACGTACCTTTGAAAATGCAATCGTCATTCTTGACGAGGCGCAGAATGTGACTGCCGCGCAAATGAAAATGTTTTTAACCCGCCTCGGGGAGAACGTGACGGTTATCGTCAACGGTGATATCACGCAATGCGATTTGCCTCGCGGCGTGTGCTCCGGATTAAGTGACGCGCTGGAACGTTTCGAAGAAGATGAAATGGTTGGGATCGTCCGGTTCGGTAAAGAGGACTGCGTACGTTCGGCACTTTGc               >  2:3599/1‑251 (MQ=255)                                                                                                                                                                                                                                      gAAAATGCCATCGTCATTCTTGACGAGGCGCAGAATGTGACTGCCGCGCAAATGAAAATGTTTTTAACCCGCCTCGGGGAGAACGTGACGGTTATCGTCAACGGTGATATCACGCAATGCGATTTGCCTCGCGGCGTGTGCTCCGGATTAAGTGACGCGCTGGAACGTTTCGAAGAAGATGAAATGGATGGGATCGTCCGGTTCGGTAAAGAGGACTGCGTACGTTCGGCACTTTGCCAACGTACGCTGc  >  1:96938/1‑250 (MQ=255)                                                                                                                                                                                                                                      gAAAATGCAATCGTCATTCTTGACGAGGCGCAGAATGTGACTGCCGCACAAATGAAAATGTTTTTAACCCGCCTCGGGGAGAACGTGACGGTTATCGTCAACGGTGATATCACGCAATGCGATTTGCCTCGCGGCGTGTGCTCCGGATTAAGTGACGCGCTGGAACGTTTCGAAGAAGATGAAATGGTTGGGATCGTCCGGTTCGGTAAAGAGGACTGCGTACGTTCGGCACTTTGACAACGTACGCTGc  >  1:242653/1‑250 (MQ=255)                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                 GATACATAAGGATGTCGACAGGATTATCGTCACCCGTCCAGTTCTGCAAGCCGATGAAGATCTTGGCTTCTTACCTGGAGATATCGCAGAAAAGTTTGCTCCCTATTTTCGCCCGGTCTACGACGTGCTGGTCCGGCGCTTAGGGGCTTCCTTTATGCAGTACTGCCTGCGACCGGAAATAGGGAAGGTGGAAATTGCGCCGTTCGCCTATATGCGTGGACGTACCTTTGAAAATGCAGTCGTCATTCTTGACGAGGCGCAGAATGTGACTGCCGCGCAAATGAAAATGTTTTTAACCCGCCTCGGGGAGAACGTGACGGTTATCGTCAACGGTGATATCACGCAATGCGATTTGCCTCGCGGCGTGTGCTCCGGATTAAGTGACGCGCTGGAACGTTTCGAAGAAGATGAAATGGTTGGGATCGTCCGGTTCGGTAAAGAGGACTGCGTACGTTCGGCACTTTGCCAACGTACGCTGC  >  NC_000913/1085564‑1086042

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑