Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	636,492	G→A	T26T (ACC→ACT)	ybdN ←	putative PAPS reductase/DUF3440 domain‑containing protein YbdN

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	636,492	0	G	A	100.0%	109.2 / NA	33	T26T (ACC→ACT)	ybdN	putative PAPS reductase/DUF3440 domain‑containing protein YbdN
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (14/19);  total (14/19)

GGTTGGTATTGTGAAAGGGAATTTTGCGTCGTAAGCGGGAGCGCAACCCAGTAAAACTCTTCGATGACATCGGTGTACAACTCGCGCAGGGACTGAACATAGTTAATGGTGCAAGAGAATTGCGCCTCCCAGTCGATAAACAAAACGCAGATTTTTTTGCCCATTTGTCGGGCAAGTTCGGCTGTCAGATGCAGCATTAAACCAGAGTCTTTGCCGCCAGAAAATGAGACACAAACGCGGGGTAGGGTGTTGAGTGTCCAGGTAATACGCTCTCGTGCAGCTTCCAGAATATTGAGCGGAAGAGGAATTTTATAAATAGACATCTGAAGACGCTCTCTGAACGTTACAGGGTGATCAGTGAGATAAGTCTGTTAATTATAAAGTAAACACTGTAAAGCAGAGTGTTTTATTTTTCATTCGCGCTGTGAATAAATAGATGAAAGTTATAGTAGCTGTCTGTAGTTATATATC  >  NC_000913/636247‑636717                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                  ggTTGGTATTGTGAAAGGGAATTTTGCGTCGTAAGCGGGAGCGCAACCCAGTAAAACTCTTCGATGACATCGGTGTACAACTCGCGCAGGGACTGAACATAGTTAATGGTGCAAGAGAATTGCGCCTCCCAGTCGATAAACAAAACGCAGATTTTTTTGCCCATTTGTCGGGCAAGTTCGGCTGTCAGATGCAGCATTAAACCAGAGTCTTTGCCGCCAGAAAATGAGACACAAACGCGGGGTAGAGTGtt                                                                                                                                                                                                                              <  2:298656/251‑1 (MQ=255)  gTTGGTATTGTGAAAGGGAATTTTGCGTCGTAAGCGGGAGCGCAACCCAGTAAAACTCTTCGATGACATCGGTGTACAACTCGCGCAGGGACTGAACATAGTTAATGGTGCAAGAGAATTGCGCCTCCCAGTCGATAAACAAAACGCAGATTTTTTTGCCCATTTGTCGGGCAAGTTCGGCTGTCAGATGCAGCATTAAACCAGAGTCTTTGCCGCCAGAAAATGAGACACAAACGCGGGGTAGAGTGTTg                                                                                                                                                                                                                             <  1:30826/251‑1 (MQ=255)        aTTGTGAAAGGGAATTTTGCGTCGTAAGCGGGAGCGCAGCCCAGTAAAACTCTTCGATGACATCGGTGTACAACTCGCGCAGGGACTGAACATAGTTAATGGTGCAAGAGAATTGCGCCTCCCAGTCGATAAACAAAACGCAGATTTTTTTGCCCATTTGTCGGGCAAGTTCGGCTGTCAGATGCAGCATTAAACCAGAGTCTTTGCCGCCAGAAAATGAGACACAAACGCGGGGTAGAGTGTTGAg                                                                                                                                                                                                                           <  1:161610/247‑1 (MQ=255)        aTTGTGAAAGGGAATTTTGCGTCGTAAGCGGGAGCGCAACCCAGTAAAACTCTTCGATGACATCGGTGTACAACTCGCGCAGGGACTGAACATAGTTAATGGTGCAAGAGAATTGCGCCTCCCAGTCGATAAACAAAACGCAGATTTTTTTGCCCATTTGTCGGGCAAGTTCGGCTGTCAGATGCAGCATTAAACCAGAGTCTTTGCCGCCAGAAAATGAGACACAAACGCGGGGTAGAGTGTTGAg                                                                                                                                                                                                                           >  2:161610/1‑247 (MQ=255)            tgAAAGGGAATTTTGCGTCGTAAGCGGGAGCGCAACCCAGTAAAACTCTTCGATGACATCGGTGTACAACTCGCGCAGGGACTGAACATAGTTAATGGTGCAAGAGAATTGCGCCTCCCAGTCGATAAACAAAACGCAGATTTTTTTGCCCATTTGTCGGGCAAGTTCGGCTGTCAGATGCAGCATTAAACCAGAGTCTTTGCCGCCAGAAAATGAGACACAAACGCGGGGTAGAGTGTTGAGTGTCCAgg                                                                                                                                                                                                                   >  2:29145/1‑251 (MQ=255)                           cgtcgtAAGCGGGAGCGCAACCCAGTAAAACTCTTCGATGACATCGGTGTACAACTCGCGCAGGGACTGAACATAGTTAATGGTGCAAGAGAATTGCGCCTCCCAGTCGATAAACAAAACGCAGATTTTTTTGCCCATTTGTCGGGCAAGTTCGGCTGTCAGATGCAGCATTAAACCAGAGTCTTTGCCGCCAGAAAATGAGACACAAACGCGGGGTAGAGTGTTGAGTGTCCAGGTAATACGCTCTCGTg                                                                                                                                                                                                    >  1:110413/1‑251 (MQ=255)                                                ccAGTAAAACTCTTCGATGACATCGGTGTACAACTCGCGCAGGGACTGAACATAGTTAATGGTGCAAGAGAATTGCGCCTCCCAGTCGATAAACAAAACGCAGATTTTTTTGCCCATTTGTCGGGCAAGTTCGGCTGTCAGATGCAGCATTAAACCAGAGTCTTTGCCGCCAGAAAATGAGACACAAACGCGGGGTAGAGTGTTGAGTGTCCAGGTAATACGCTCTCGTGCAGCTTCCAGAATATTGAGCg                                                                                                                                                                               >  2:293443/1‑251 (MQ=255)                                                     aaaaCTCTTCGATGACATCGGTGTACAACTCGCGCAGGGACTGAACATAGTTAATGGTGCAAGAGAATTGCGCCTCCCAGTCGATAAACAAAACGCAGATTTTTTTGCCCATTTGTCGGGCAAGTTCGGCTGTCAGATGCAGCATTAAACCAGAGTCTTTGCCGCCAGAAAATGAGACACAAACGCGGGGTAGAGTGTTGAGTGTCCAGGTAATACGCTCTCGTGCAGCTTCCAGAATATTGAGCGGAag                                                                                                                                                                           <  2:110413/250‑1 (MQ=255)                                                      aaaCTCTTCGATGACATCGGTGTACAACTCGCGCAGGGACTGAACTTAGTTAATGGTGCAAGAGAATTGCGCCTCCCAGTCGATAAACAAAACGCAGATTTTTTTGCCCATTTGTCGGGCAAGTTCGGCTGTCAGATGCAGCATTAAACCCGCGTCTTTGCCGCCAGAAAATGAGACACAAACGCGGGGTAGAGTGTTGAGTGTCCAGGTAATACGCTCTCGTGCAGCTTCCAGAATTTTCAGCGGa                                                                                                                                                                             >  2:306405/1‑247 (MQ=255)                                                                   aCATCGGTGTACAACTCGCGCAGGGACTGAACATAGTTAATGGTGCAAGAGAATTGCGCCTCCCAGTCGATAAACAAAACGCAGATTTTTTTGCCCATTTGTCGGGCAAGTTCGGCTGTCAGATGCAGCATTAAACCAGAGTCTTTGCCGCCAGAAAATGAGACACAAACGCGGGGTAGAGTGTTGAGTGTCCAGGTAATACGCTCTCGTGCAGCTTCCAGAATATTGAGCGGAAGAGGAATTTTATAAAt                                                                                                                                                            <  1:863/251‑1 (MQ=255)                                                                                                aaCATAGTTAATGGTGCAAGAGAATTGCGCCTCCCAGTCGATAAACAAAACGCAGATTTTTTTGCCCATTTGTCGGGCAAGTTCGGCTGTCAGATGCAGCATTAAACCAGAGTCTTTGCCGCCAGAAAATGAGACACAAACGCGGGGTAGAGTGTTGAGTGTCCAGGTAATACGCTCTCGTGCAGCTTCCAGAATATTGAGCGGAAGAGGAATTTTATAAATAGACATCTGAAGACGCTCTCTGAACGTTa                                                                                                                               <  1:105692/251‑1 (MQ=255)                                                                                                 aCATAGTTAATTGTGCAAGAGAATTGCGCCTCCCAGTCTATAAACAAAACGCAGATTTTTTTGCCCATGTGTCGGGCAAGTTCGGCTGTCAGATGCAGCATTAAACCAGAGTCGTGGCCGCCAGAAAATGAGACACAAACGCGGGGTAGAGTGTTGAGTGTCCAGGTAATACGCTCTCGTGCAGCTTCCAGAATATTGAGCGGAAGAGGAATTTTATAAATAGACATCTGAAGACGCTCTCTGACCGtaac                                                                                                                              <  2:129756/251‑4 (MQ=255)                                                                                                  cATAGTTAATGGTGCAAGAGAATTGCGCCTCCCAGTCGATAAACAAAACGCAGATTTTTTTGCCCATTTGTCGGGCAAGTTCGGCTGTCAGATGCAGCATTAAACCAGAGTCTTTGCCGCCAGAAAATGAGACACAAACGCGGGGTAGAGTGTTGAGTGTCCAGGTAATACGCTCTCGTGAAGCTTCCAGAATATTGAGCGGAAGAGGAATTTTATAAATAGACATCTGAAGACGCTCTCTGAACGTTAc                                                                                                                              >  1:31770/1‑250 (MQ=255)                                                                                                     aGTTAATGGTGCAAGAGAATTGCGCCTCCCAGTCGATAAACAAAACGCAGATTTTTTTGCCCATTTGTCGGGCAAGTTCGGCTGTCAGATGCAGCATTAAACCAGAGTCTTTGCCGCCAGAAAATGAGACACAAACGCGGGGTAGAGTGTTGAGTGTCCAGGTAATACGCTCTCGTGCAGCTTCCAGAATATTGAGCGGAAGAGGAATTTTATAAATAGACATCTGAAGACGCTCTCTGAACGTTACAggg                                                                                                                          <  1:450051/251‑1 (MQ=255)                                                                                                             gTGCAAGAGAATTGCGCCTCCCAGTCGATAAACAAAACGCAGATTTTTTTGCCCATTTGTCGGGCAAGTTCGGCTGTCAGATGCAGCATTAAACCAGAGTCTTTGCCGCCAGAAAATGAGACACAAACGCGGGGTAGAGTGTTGAGTGTCCAGGTAATACGCTCTCGTGCAGCTTCCAGAATATTGAGCGGAAGAGGAATTTTATAAATAGACATCTGCAGACGCTCTCTGAACGTTACAGGGTGATCAGt                                                                                                                  >  1:270685/1‑251 (MQ=255)                                                                                                                ccagagAATTGCGCCTCCCAGTCGATAAACAAAACGCAGATTTTTTTGCCCATTTGTCGGGCAAGTTCGGCTGTCAGATGCAGCATTAAACCAGAGTCTTTGCCGCCAGAAAATGAGACACAAACGCGGGGTAGAGTGTTGAGTGTCCAGGTAATACGCTCTCGTGCAGCTTCCAGAATATTGCGCGGAAGAGGAATTTTATAAATATACCTCTGAAGACGATCTCTGAACGTTACAGGGTGATCAGTg                                                                                                                 >  2:203983/3‑249 (MQ=255)                                                                                                                   gagaATTGCGCCTCCCAGTCGATAAACAAAACGCAGATTTTTTTGCCCATTTGTCGGGCAAGTTCGGCTGTCAGATGCAGCATTAAACCAGAGTCTTTGCCGCCAGAAAATGAGACACAAACGCGGGGTAGAGTGTTGAGTGTCCAGGTAATACGCTCTCGTGCAGCTTCCAGAATATTGAGCGGAAGAGGAATTTTATAAATAGACATCTGAAGACGCTCTCTGAACGTTACAGGGTGATCAGTGAGATa                                                                                                            <  2:24332/251‑1 (MQ=255)                                                                                                                          gcgcCTCCCAGTCAATAAACAAAACGCAGATTTTTTTGCCCATTTGTCGGGCAAGTTCGGCTGTCAGATGCAGCAGTAAGCCATAGTCTTGTCCGCCAGAAAATGAGACACAACCGCGGTGTAGAGTGTTGAGTGTCCAGGTAATACGCTCTCGTGCAGCTTCCATAATATTGAGTGGAAGAGGAATTTTATAAATAGACTGCTGAAGACGCTCTCTGAACGTTACAGGGTGATCAGTGAGATAAGTCTGt                                                                                                     <  1:63664/251‑1 (MQ=255)                                                                                                                                cccAGTCGATAAACAAAACGCAGATTTTTTTGCCCATTTGTCGGGCAAGTTCGGCTGTCAGATGCAGCATTAAACCAGAGTCTTTGCCGCCAGAAAATGAGACACAAACGCGGGGTAGAGTGTTGAGTGTCCAGGTAATACGCTCTCGTGCAGCTTCCAGAATATTGAGCGGAAGAGGAATTTTATAAATAGACATCTGAAGACGCTCTCTGAACGTTACAGGGTGATCAGTGAGATAAGTCTGTTAAtt                                                                                                <  1:293443/250‑1 (MQ=255)                                                                                                                                           aaCAAAACGCAGATTTTTTTGCCCATTTGTCGGGCAAGTTCGGCTGTCAGATGCAGCATTAAACCAGAGTCTTTGCCGCCAGAAAATGAGACACAAACGCGGGGTAGAGTGTTGAGTGTCCAGGTAATACGCTCTCGTGCAGCTTCCAGAATATTGAGCGGAAGAGGAATTTTATAAATAGACATCTGAAGACGCTCTCTGAACGTTACAGGGTGATCAGTGAGATAAGTCTGTTAATTATAAAGTAAaca                                                                                    <  2:324226/251‑1 (MQ=255)                                                                                                                                                            tttGCCCATTTGTCGGGCAAGTTCGGCTGTCAGATGCAGCATTAAACCAGAGTCGTTGCCGCCAGAAAATGAGACACAAACGCGGGTTAGAGTGTTAAGTGTCCAGGTAATACGCTCGCGTGCAGCTTCCAGAAGATTGAGCGGAAGAGGAATTTTATAAATAGACATCTGAAGACGCTCTCTGAACGTTACAGCGTGATCAGTGAGATAATTCTGTTAATTATAAAGTAAACCCTGTAAAGCAGAgtgt                                                                    <  1:51281/250‑1 (MQ=255)                                                                                                                                                                 ccATTTGTCGGGCAAGTTCGGCTGTCAGATGCAGCATTAAACCAGAGTCTTTGCCGCCAGAAAATGAGACACAAACGCGGGGTAGAGTGTTGAGTGTCCAGGTAATACGCTCTCGTGCAGCTTCCAGAATATTGAGCGGAAGAGGAATTTTATAAATAGACATCTGAAGACGCTCTCTGAACGTTACAGGGTGATCAGTGAGATAAGTCTGTTAATTATAAAGTAAACACTGTAAAGCAGAGTGTTTTAtt                                                              >  1:448154/1‑251 (MQ=255)                                                                                                                                                                      tGTCGGGCAAGTTCGGCTGTCAGATGCAGCATTAAACCAGAGTCTTTGCCGCCAGAAAATGAGACACAAACGCGGGGTAGAGTGTTGAGTGTCCAGGTAATACGCTCTCGTGCAGCTTCCAGAATATTGAGCGGAAGAGGAATTTTATAAATAGACATCTGAAGACGCTCTCTGAACGTTACAGGGTGATCAGTGAGATAAGTCTGTTAATTATAAAGTAAACACTGTAAAGCAGAGTGTTTTATTTTTc                                                          <  1:29145/250‑1 (MQ=255)                                                                                                                                                                             cAAGTTCGGCTGTCAGATGCAGCATTAAACCAGAGTCTTTGCCGCCAGAAAATGAGACACAAACGCGGGGTAGAGTGTTGAGTGTCCAGGTAATACGCTCTCGTGCAGCTTCCAGAATATTGAGCGGAAGAGGAATTTTATAAATAGACATCTGAAGACGCTCTCTGAACGTTACAGGGTGATCAGTGAGATAAGTCTGTTAATTATAAAGTaaa                                                                                      <  1:94012/215‑1 (MQ=255)                                                                                                                                                                             cAAGTTCGGCTGTCAGATGCAGCATTAAACCAGAGTCTTTGCCGCCAGAAAATGAGACACAAACGCGGGGTAGAGTGTTGAGTGTCCAGGTAATACGCTCTCGTGCAGCTTCCAGAATATTGAGCGGAAGAGGAATTTTATAAATAGACATCTGAAGACGCTCTCTGAACGTTACAGGGTGATCAGTGAGATAAGTCTGTTAATTATAAAGTaaa                                                                                      >  2:94012/1‑215 (MQ=255)                                                                                                                                                                               aGTTCGGCTGTCAGATGCAGCATTAAACCAGAGTCTTTGCCGCCAGAAAATGAGACACAAACGCGGGGTAGAGTGTTGAGTGTCCAGGTAATACGCTCTCGTGCAGCTTCCAGAATATTGAGCGGAAGAGGAATTTTATAAATAGACATCTGAAGACGCTCTCTGAACGTTACAGGGTGATCAGTGAGATAAGTCTGTTAATTATAAAGTAAACACTGTAAAGCAGAGTGTTTTATTTTTCATTCGCGCtg                                                >  2:316425/1‑251 (MQ=255)                                                                                                                                                                                    ggCTGTCAGATGCAGCATTAAACCAGAGTCCTTGCCGCCAGAAAATGAGACACAAACGCGGGGTAGAGTGTTGAGTGTCCAGGTAATACGCTCTCGTGCAGCTTCCAGAATATTGAGCGGAAGAGGAATTTTATAAATAGATATCTGAAGACGCTCTCTGAACGTTACTAGGTGATCAGTGAGATAAGTCTGGTAATTATAAAGTAAAGAATGTAAAGCAGAGTGTGTTGTTTTTCATTCTCGCTGTGaat                                           <  2:438579/251‑1 (MQ=255)                                                                                                                                                                                                          ccAGAGTCTTTGCCGCCAGAAAATGAGACACAAACGCGGGGTAGAGTGTTGAGTGTCCAGGTAATACGCTCTCGTGCAGCTTCCAGAATATTGAGCGGAAGAGGAATTTTATAAATAGACATCTGAAGACGCTCTCTGAACGTTACAGGGTGATCAGTGAGATAAGTCTGTTAATTATAAAGTAAACACTGTAAAGCAGAGTGTTTTATTTTTCATTCGCGCTGTGAATAAATAGATGAAAGTTAtagt                       >  1:70446/1‑249 (MQ=255)                                                                                                                                                                                                             gagTCTTTGCCGCCAGAAAATGAGACACAAACGCGGGGTAGAGTGTTGAGTGTCCAGGTAATACGCTCTCGTGCAGCTTCCAGAATATTGAGCGGAAGAGGAATTTTATAAATAGACATCTGAAGACGCTCTCTGAACGTTACAGGGTGATCAGTGAGATAAGTCTGTTAATTATAAAGTAAACACTGTAAAGCAGAGTGTTTTATTTTTCATTCGCGCTGTGAATAAATAGATGAAAGTTATAGTAGctg                  <  2:111588/251‑1 (MQ=255)                                                                                                                                                                                                                tCTTTGCCGCCAGAAAATGAGACACAAACGCGGGGTAGAGTGTTGAGTGTCCAGGTAATGCGCTCTCGTGCAGCTTCCAGAATATTGAGCGGAAGAGGAATTTTATAAATAGa                                                                                                                                                         <  1:289441/113‑1 (MQ=255)                                                                                                                                                                                                                tCTTTGCCGCCAGAAAATGAGAAACAAACGCGGGGTAGAGTGTTGAGTGTCCAGGTAATACGCTCTCGTGCAGCTTCCAGAATATTGAGCGGAAGAGGAATTTTATAAATAGa                                                                                                                                                         >  2:289441/1‑113 (MQ=255)                                                                                                                                                                                                                           aGAACATGAGACACAAACGCGGGGTAGAGGGTTGAGTGTCCAGGTAATACGCTATCGTGCAGCTTCCAGAATATTGAGCGGAAGAGGAATTTTATAAATAAACATCTGAAGACGCTCTCTGAACGTTACAGGGTGATCAGTGAGATAAGTCTGTTAATTATAAAGTAAACACTGTAAAGCAGAGTGTTTTATTTTTCATTCGCGCTGTGAATAAATAGATGAAAGTAATAGTAACTGTCTGTAGTtanat     >  2:412884/1‑250 (MQ=255)                                                                                                                                                                                                                             aaaaTGAGACACAAACGCGGGGTAGAGTGTTGAGTGTCCAGGTAATACGCTCTCGTGCAGCTTCCAGAATATTGAGCGGAAGAGGAATTTTATAAATAGACATCTGAAGACGCTCTCTGAACGTTACAGGGTGATCAGTGAGATAAGTCTGTTAATTATAAAGTAAACACTGTAAAGCAGAGTGTTTTATTTTTCATTCGCGCTGTGAATAAATAGATGAAAGTTATAGTAGCTGTCTGTAGTTATATATc  <  2:139476/251‑1 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                  GGTTGGTATTGTGAAAGGGAATTTTGCGTCGTAAGCGGGAGCGCAACCCAGTAAAACTCTTCGATGACATCGGTGTACAACTCGCGCAGGGACTGAACATAGTTAATGGTGCAAGAGAATTGCGCCTCCCAGTCGATAAACAAAACGCAGATTTTTTTGCCCATTTGTCGGGCAAGTTCGGCTGTCAGATGCAGCATTAAACCAGAGTCTTTGCCGCCAGAAAATGAGACACAAACGCGGGGTAGGGTGTTGAGTGTCCAGGTAATACGCTCTCGTGCAGCTTCCAGAATATTGAGCGGAAGAGGAATTTTATAAATAGACATCTGAAGACGCTCTCTGAACGTTACAGGGTGATCAGTGAGATAAGTCTGTTAATTATAAAGTAAACACTGTAAAGCAGAGTGTTTTATTTTTCATTCGCGCTGTGAATAAATAGATGAAAGTTATAGTAGCTGTCTGTAGTTATATATC  >  NC_000913/636247‑636717

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑