Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,124,889	G→A	A146A (GCC→GCT)	mdtH ←	multidrug efflux pump MdtH

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,124,889	0	G	A	100.0%	170.0 / NA	49	A146A (GCC→GCT)	mdtH	multidrug efflux pump MdtH
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (22/27);  total (22/27)

ATCACTTGTACAGCCAGCATGTAGTAACCCGCCAGCGTCAGAACATAGGTGACAAAACGCTTGTCACGCATCACGCGGGTCATGCCTTCGCGAACGGGCGTGCGTACGGTGGAGAGTTTCCATGCTGGTAACAACCACGCATTGAACGCCGCACATAGCACAAATAGAACTGCCCCTGTGGCGCAGACCAGGCGAAAGTCGTATTGCAACAGCCAGCTCCCCAACAATGCGCCAATGACCGCACCGGCACTGTCCTGCATCATCAACAGCGAGAAAAAACGACCA‑CGCTGCTGTGGACGGATTAATTTCACCACCAGCGCCGAACGCGGCGGATCAAACAACGTGCCACCGAGTCCCGAGAGCAGGCATGAAAACCACAATAGCCACGGTTCGTGGGCGATACCCATTGTGGCGAATCCGGCGGCGCGCATCAGCATACCGGTAACAATCATCGGTTTGGCACCAAAGCGGTCGGCAATTGCACCGCCGA  >  NC_000913/1124644‑1125133                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                      atcaCTTGTACAGCCAGCATGTAGTAACCCGCCAGCGTCAGAACATAGGTGACAAAACGCTTGTCACGCATCACGCGGGTCATGCCTTCGCGAACGGGCGTGCGTACGGTGGAGAGTTTCCATGCTGGTAACAACCACGCATTGAACGCCGCACATAGCACAAATAGAACTGCCCCTGTGGCGCAGACCAGGCGAAAGTCGTATTGCAACAGCCAGCTCCCCAACAATGCGCCAATGACCGCACCAGCACt                                                                                                                                                                                                                                                  >  2:39738/1‑251 (MQ=255)            agccagcATGTAGTAACCCGCCAGCGTCAGAACATAGGTGACAAAACGCTTGTCACGCATCACGCGGGTCATGCCTTCGCGAACGGGCGTGCGTACGGTGGAGAGCTTCCATGCTGGTAACAACCACGCATTGAACGCCGCACATAGCACAAATAGAACTGCCCCTGTGGCGCAGACCAGGCGAAAGTCGTATTGCAACAGCCAGCTCCCCAACAATGCGCCAATGACCGCACCAGCACTGTCCTGcatc                                                                                                                                                                                                                                        >  1:104524/1‑250 (MQ=255)                   atggagcaaCCCGCCAGCGTCAGAACATAGGTGACAAAACGCTTGTCACGCAGCACGCGGGTCATGCCTGCGCGAACGGGCGTGCGTACGGTGGAGAGTTTCCATGCTGGTAACAACCACGCATTGAACGCCGCACATAGCACAAATAGAACTGCCCCGGTGGCGCAGACCAGGCGAAAGGCGTATTGCAACAGCCAGCTCCCCAACAATGCGCCAATGACCGCACCAGCACTGTCCTGCATCATCAACa                                                                                                                                                                                                                                 <  2:20196/243‑1 (MQ=255)                                 cAGCGTCAGAACATAGGTGACAAAACGCTTGTCACGCATCACGCGGGTCATGCCTTCGCGAACGGGCGTGCGTACGGTGGAGAGTTTCCATGCTGGTAACAACCACGCATTGAACGCCGCACATAGCACAAATAGAACTGCCCCTGTGGCGCAGACCAGGCGAAAGTCGTATTGCAACAGCCAGCTCCCCAACAATGCGCCAATGACCGCACCAGCACTGTCCTGCATCATCAACAGCGAGAAAAAACGAc                                                                                                                                                                                                                  <  1:171049/251‑1 (MQ=255)                                    cGTCAGAACATAGGTGACAAAACGCTTGTCACGCATCACGCGGGTCATGCCTTCGCGAACGGGCGTGCGTACGGTGGAGAGTTTCCATGCTGGTAACAACCACGCATTGAACGCCGCACATAGCACAAATAGAACTGCCCCTGTGGCGCAGACCAGGCGAAAGTCGTATTGCAACAGCCAGCTCCCCAACAATGCGCCAATGACCGCACCAGCACTGTCCTGCATCATCAACAGCGAGAAAAAACGACCA‑c                                                                                                                                                                                                              <  1:424720/251‑1 (MQ=255)                                               aGGTGACAAAACGCTTGTCACGCATCACGCGGGTCATGCCTTCGCGAACGGGCGTGCGTACGGTGGAGAGTTTCCATGCTGGTAACAACCACGCATTGAACGCCGCACATAGCACAAATAGAACTGCCCCTGTGGCGCAGACCAGGCGAAAGTCGTATTGCAACAGCCAGCTCCCCAACAATGCGCCAATGACCGCACCAGCACTGTCCTGCATCATCAACAGCGAGAAAAAACGACCA‑CGCTGCTGTgg                                                                                                                                                                                                    >  1:445867/1‑250 (MQ=255)                                                ggTGACAAAACGCTTGTCACGCATCACGCGGGTCATGCCTTCGCGAACGGGCGTGCGTACGGTGGAGAGTTTCCATGCTGGTAACAACCACGCATTGAACGCCGCACATAGCACAAATAGAACTGCCCCTGTGGCGCAGACCAGGCGAAAGTCGTATTGCAACAGCCAGCTCCCCAACAATGCGCCAATGACCGCACCAGCACTGTCCTGCATCATCAACAGCGAGAAAAAACGACCA‑CGCTGCTGTGGAc                                                                                                                                                                                                  >  1:148052/1‑251 (MQ=255)                                                 gTGACAAAACGCTTGTCACGCATCACGCGGGTCATGCCTTCGCGAACGGGCGTGCGTACGGTGGAGAGTTTCCATGCTGGTAACAACCACGCATTGAACGCCGCACATAGCACAAATAGAACTGCCCCTGTGGCGCAGACCAGGCGAAAGTCGTATTGCAACAGCCAGCTCCCCAACAATGCGCCAATGACCGCACCAGCACTGTCCTGCATCATCAACAGCGAGAAAAAACGACCA‑CGCTGCTGTGGACg                                                                                                                                                                                                 <  2:80842/251‑1 (MQ=255)                                                     cAAAACGCTTGTCACGCATCAAGCGGGTCATGCCTTCGCGAACGGGCGTGCGTTCCGCGGAGAGTTTCCATGCTGGTAACAACCACGCATTGGACGCCGCACATAGCACAAATAGACCTGCCCATGTGGCGAAGACCAGGCGAAAGTCGTATTGCAACAGCCAGCTCCCCAACAATGCGCCAATGACTGCACCAGCACTGTCCTGCATCATCAACAGCGAGAAAAAAC‑ACCAACGCTCCTGTGTACGGAtt                                                                                                                                                                                             >  1:266613/1‑251 (MQ=255)                                                      aaaaCGCTTGTCACGCATCACGCGGGTCATGCCTTCGCGAACGGGCGTGCGTACGGTGGAGAGTTTCCATGCTGGTAACAACCACGCATTGAACGCCGCACATAGCACAAATAGAACTGCCCCTGTGGCGCAGACCAGGCGAAAGTCGTATTGCAACAGCCAGCTCCCCAACAATGCGCCAATGACCGCACCAGCACTGTCCTGCATCATCAACAGCGAGAAAAAACGACCA‑CGCTGCTGTGGACGGATTa                                                                                                                                                                                            <  2:43521/251‑1 (MQ=255)                                                         aCGCTTGTCACGCATCACGCGGGTCATGCCTTCGCGAACGGGCGTGCGTACGGTGGAGAGTTTCCATGCTGGTAACAACCACGCATTGAACGCCGCACATAGCACAAATAGAACTGCCCCTGTGGCGCAGACCAGGCGAAAGTCGTATTGCAACAGCCAGCTCCCCAACAATGCGCCAATGACCGCACCAGCACTGTCCTGCATCATCAACAGCGAGAAAAAACGACCA‑CGCTGCTGTGGACGGATTAAtt                                                                                                                                                                                         >  1:431903/1‑251 (MQ=255)                                                          cGCTTGTCACGCATCACGCGGGTCATGCCTTCGCGAACGGGCGTGCGTACGGTGGAGAGTTTCCATGCTGGTAACAACCACGCATTGAACGCCGCACATAGCACAAATAGAACTGCCCCTGTGGCGCAGACCAGGCGAAAGTCGTATTGCAACAGCCAGCTCCCCAACAATGCGCCAATGACCGCACCAGCACTGTCCTGCATCATCAACAGCGAGAAAAAACGACCA‑CGCTGCTGTGGACGGATTAAttt                                                                                                                                                                                        <  1:423439/251‑1 (MQ=255)                                                                    gCATCACTCGGGTCATGCCTTCGCGAACGGGCGTGCGTACGGTGGATCGTTTCCATGCTGGTAACAACCACGCATTGAACGCCGCACATAGCACAAATAGAACTGCCCCTGTGGCGCAGACCAGGCGAAAGTCGTATTGCAACACCCAGCTCCCCAACAATGCGCCAATGACCGCACCAGCACTGTCCTGCTTCATCAACAGCGAGAAAAAACGACCA‑CGCTGCTGTGGACGGATTAATTTCACCACCAgc                                                                                                                                                                              <  1:38784/251‑1 (MQ=255)                                                                            ggggTCATGCCTTCGCGAACGGGCGTGCGTACGGTGGAGAGTTTCCATGCTGGGAACAACCGCGCATTGAACGCCGCACATAGCACAAATAGAACTGCCCCTGTGGCGCAGACCAGGCGAAAGTCGTATTGCAACAGCCAGCTCCCCAACAATGCGCCAATGACCGCACCAGCACTGTCCTGCATCATCAACAGCGAGAAAAAACGACCA‑CGCTGCTGTGGACGGATTAATTTCACCACCAGCGCCGAAcg                                                                                                                                                                      <  1:441862/250‑1 (MQ=255)                                                                            cgGGTCATGCCTTCGCGAACGGGCGTGCGTACGGTGGAGAGTTTCCATGCTGGTAACAACCACGCATTGAACGCCGCACATAGCACAAATAGAACTGCCCCTGTGGCGCAGACCAGGCGAAAGTCGTATTGCAACAGCCAGCTCCCCAACAATGCGCCAATGACCGCACCAGCACTGTCCTGCATCATCAACAGCGAGAAAAAACGACCA‑CGCTGCTGTGGACGGATTAATTTCACCACCAGCGCCGAAcg                                                                                                                                                                      <  1:41332/251‑1 (MQ=255)                                                                                      cTTCGCGAACGGGCGTGCGTACGGTGGAGAGTTTCCATGCTGGTAACAACCACGCATTGAACGCCGCACATAGCACAAATAGAACTGCCCCTGTGGCGCAGACCAGGCGAAAGTCGTATTGCAACAGCCAGCTCCCCAACAATGCGCCAATGACCGCACCAGCACTGTCCTGCATCATCAACAGTGAGAAAAAACGACCA‑CGCTGCTGTGGACGGATTAATTTCACCACCAGCGCCGAACGCGGCGGATc                                                                                                                                                             >  2:392563/1‑250 (MQ=255)                                                                                        tCGCGAACGGGCGTGCGTACGGTGGAGAGTTTCCATGCTGGTAACAACCACGCATTGAACGCCGCACATAGCACAAATAGAACTGCCCCTGTGGCGCAGACCAGGCGAAAGTCGTATTGCAACAGCCAGCTCCCCAACAATGCGCCAATGACCGCACCAGCACTGTCCTGCATCATCAACAGCGAGAAAAAACGACCA‑CGCTGCTGTGGACGGATTAATTTCACCACCAGCGCCGAACGCGGCGGATCa                                                                                                                                                            <  1:104740/249‑1 (MQ=255)                                                                                         cgcgAACGGGCGTGCCTACGGTGGAGAGTTTCCATTCTGGTAACAACCACGCATTGAACGCCGCACATAGCAGAAATAGAACTGCCCCGGTGGCGCAGACCAGGCGAAACTCGTATTGCAACAGCCAGCTCCCCAACAATGCGCCAATGGCCGCACCAGCACTGTCCTGCATCATCAACAGCGAGAAAAAACGACCT‑CGCTGCTGTGGACGGATTAATTTCACCACCAGCGCCGAACACGGCAGATCaaa                                                                                                                                                          <  2:431903/250‑1 (MQ=255)                                                                                             aaCGGGCGGGCGTACGGTGGAGAGTTTCCATGCTGGTAACAACCACGCATTGAACGCCGCACATAGCACAAATAGAACTGCCCCTGTGGCGCAGACCAGGCGAAAGTCGTATTGCAACAGCCAGCTCCCCAACAATGCGCCAATGACCGCACCAGCACTGTCCTGCATCATCAACAGCGAGAACAAACGACCA‑CGCTGCTGTGGACGGCTTAATTTCACCACCAGCGCCGAACGCGGCGGAGCACACAACg                                                                                                                                                     >  2:256855/1‑251 (MQ=255)                                                                                                  gcgtgcgtACGGTGGAGAGTTTCCATGCTGGTAACAACCACGCATTGAACGCCGCACATAGCACAAATAGAACTGCCCCTGTGGCGCAGACCAGGCGAAAGTCGTATTGCAACAGCCAGCTCCCCAACAATGCGCCAATGACCGCACCAGCACTGTCCTGCATCATCAACAGCGAGAAAAAACGACCA‑CGCTGCTGTGGACGGATTAATTTCACCACCAGCGCCGAACGCGGCGGATCAAACAACGTGCCa                                                                                                                                                <  2:148052/251‑1 (MQ=255)                                                                                                    gtgcgtACGGTGGAGAGTTTCCATGCTGGTAACAACCACGCATTGAACGCCGCACATAGCACAAATAGAACTGCCCCTGTGGCGCAGACCAGGCGAAAGTCGTATTGCAACAGCCAGCACCCCAACAATGCGCCAATGACCGCACCAGCACTGTCCTGCATCATCAACAGCGAGAAAAAACGACCA‑CGCTGCTGTGGACGGATTAATTTCACCGCCAGCGCCGAACGCGGCTGATCAAACAACGTGCCAcc                                                                                                                                              <  1:126991/251‑1 (MQ=255)                                                                                                       cgtACGGTGGAGAGTTTCCATGCTGGTAACAACCACGCATTGAACGCCGCACATAGCACAAATAGAACTGCCCCTGTGGCGCAGACCAGGCGAAAGTCGTATTGCAACAGCCAGCTCCCCAACAATGCGCCAATGACCGCACCAGCACTGGCCTGCATCATCAACAGCGAGAAAAAACGACCA‑CGCTGCTGTGGACGGATTAATTTCACCACCAGCGCCGAACGCGGCGGATCAAACAACGTGCCACCGAg                                                                                                                                           <  1:271289/251‑1 (MQ=255)                                                                                                                   agTTTCCATGCTGGTAACAACCACGCATTGAACGCCGCACATAGCACAAATAGAACTGCCCCTGTGGCGCAGACCAGGCTAAAGTCGTATTGCAACAGCCAGCTCCCCAACAATGCGCCAATGACCGCACCAGCACTGTCCTGCATCATCAACAGCGAGAAAAAACGACCA‑CGCTGCTGTGGACGGATTAATTTCACCCCCAGCGCCGAACGCGGCGGATCAAACAACGTGCCACCGAGTCCCGAGAGCat                                                                                                                               <  2:133757/251‑2 (MQ=255)                                                                                                                      ttCCATGCTGGTAACAACCACGCATTGAACGCCGCACATAGCACAAATAGAACTGCCCCTGTGGCGCAGACCAGGCGAAAGTCGTATTGCAACAGCCAGCTCCCCAACAATGCGCCAATGACCGCACCAGCACTGTCCTGCATCATCAACAGCGAGAAAAAACGACCA‑CGCTGCTGTGGACGGATTAATTTCACCACCAGCGCCGAACGCGGCGGATCAAACAACGTGCCACCGAGTCCCGAGAGCAGGc                                                                                                                             >  1:107460/1‑250 (MQ=255)                                                                                                                        ccaggttGGTAACAACCCCGCATTGAACGCCGCACATAGCACAAAAAGAACTGCCCCTGTGGCGCAGACCAGGCGAAAGTCGTAGTGCAACAGCCAGCTCCCCAACAATGCGCCAATGACCGCACCAGCACTTGCCTTCATCATCAACAGCGAGAAAAAACGACCA‑CGCTGCCTTGGACGGATCAATTTCACCACCAGCGCCGAATGCGTCGGATCAAACAACGTGCCACCGAGTCCCGAGAGCAGGCATg                                                                                                                          <  1:411611/245‑1 (MQ=255)                                                                                                                          aTGCTGGTAACAACCACGCATTGAACGCCGCACATAGCACAAATAGAACTGCCCCTGTGGCGCAGACCAGGCGAAAGTCGTATTGCAACAGCCAGCTCCCCAACAATGCGCCAATGACCGCACCAGCACTGTCCTGCATCATCAACAGCGAGAAAAAACGACCA‑CGCTGCTGTGGACGGATTAATTTCACCACCAGCGCCGAACGCGGCGGATCAAACAACGTGCCACCGAGTCCCGAGATCAGGCATGaa                                                                                                                        <  2:402410/251‑1 (MQ=255)                                                                                                                           tGCTGGTAACACCCACGCATTGAACGCCGCACATAGCACAAATAGAACTGCCCCTGTGGCGCAGACCAGGCGAAAGTCGTATTGCAACAGCCAGCTCCCCAACAATGCGCCAATGACCGCACCAGCACTGTCCTGCATCATCAACAGCGAGAAAAAACGACCA‑CGCTGCTGTGGACGGATTAATTTCACCACCAGCGCCGAACGCGGCGGATCAAACAACGTGCCACCGAGTCCCGAGAGCa                                                                                                                                <  2:53188/242‑1 (MQ=255)                                                                                                                           tGCTGGTAACAACCACGCATTGAACGCCGCACATAGCACAAATAGAACTGCCCCTGTGGCGCAGACCAGGCGAAAGTCGTATTGCAACAGCCAGCTCCCCAACAATGCGCCAATGACCGCACCAGCACTGTCCTGCATCATCAACAGCGAGAAAAAACGACCA‑CGCTGCTGTGGACGGATTAATTTCACCACCAGCGCCGAACGCGGCGGATCAAACAACGTGCCACCGAGTCCCGAGAGCa                                                                                                                                >  1:53188/1‑242 (MQ=255)                                                                                                                              tGGTAACCAACACGCATTGAACGCCGCACATAGCACAAATAGACCTGACCCTGTGGCGCAGACCAGGCGAAAGTCGTATTGCAACAGCCAGCTCCCCAACAATGCGCCAATGGCCGCACCAGCACTGTCCTGCATCATCAACAGCGAGAAAAAACGACCA‑CGCTGCTGTGGACGGATTACTTTCACCACCAGCGCCGAACGCGGCGGATCAAACAACGTGCCACCGCGTACCGAGAGCAGGCATGAAAAcc                                                                                                                    >  2:377854/1‑251 (MQ=255)                                                                                                                                     aacCACGCATTGAACGCCGCACATAGCACAAATAGAACTGCCCCTGTGGCGCAGACCAGGCGAAAGTCGTATTGCAACAGCCAGCTCCCCAACAATGCGCCAATGACCGCACCAGCACTGTCCTGCATCATCAACAGCGAGAAAAAACGACCA‑CGCTGCTGTGGACGGATTAATTTCACCACCAGCGCCGAACGCGGCGGATCAAACAACGTGCCACCGAGTCCCGAGAGCAGGCATGAAAACCACAAt                                                                                                               >  2:129037/1‑249 (MQ=255)                                                                                                                                          cGCATTGAACGCCGCACATAGCACAAATAGAACTGCCCCTGTGGCGCAGACCAGGCGAAAGTCGTATTGCAACAGCCAGCTCCCCAACAATGCGCCAATGACCGCACCAGCACTGTCCTGCATCATCAACAGCGAGAAAAAACGACCA‑CGCTGCTGTGGACGGATTAATTTCACCACCAGCGCCGAACGCGGCGGATCAAACAACGTGCCACCGAGTCCCGAGAGCAGGCATGAAAACCACAATAGCCACg                                                                                                        >  1:208010/1‑251 (MQ=255)                                                                                                                                           gCATTGAACGCCGCACATAGCACAAATAGAACTGCCCCTGTGGCGCAGACCAGGCGAAAGTCGTATTGCAACAGCCAGCTCCCCAACAATGCGCCAATGACCGCACCAGCACTGTCCTGCATCATCAACAGCGAGAAAAAACGACCA‑CGCTGCTGTGGACGGATTAATTTCACCACCAGCGCCGAACGCGGCGGATCAAACAACGTGCCACCGAGTCCCGAGAGCAGGCATGAAAACCACAATAGCCACgg                                                                                                       >  1:78937/1‑251 (MQ=255)                                                                                                                                            ctcttccgatcccacaTAGCACAAATAGAACTGCCCCTGTGGCGCAGACCAGGCGAAAGTCGTATTGCAACAGCCAGCTCCCCAACAAAGCGCCAATGCCCGCCCCAGCACTGTCCTGCATCATCAACAGCGAGAAAAAGCGGCCA‑CGCTGCTGTGGACGGATTAATTTCGCCACCAGCGCCGAACGCGGCGGATCAAACAACGTGCCACCGACTCCCGAGAGCAGGCATGAAAACCACAATAGCCACg                                                                                                        <  2:291597/237‑1 (MQ=255)                                                                                                                                            cATTGAACGCCGCACATAGCACAAATAGAACTGCCCCTATGGCGCAGACCAGGCGAAAGACGTATTGAAACAGCCAGCTCCCCAACAATGCGCCAATGACCGCACCAGCACTGTCCTGCATCATCAACAGCGAGAAAAAACGACCA‑CGCTGCTGTGGACGGATTAATTTCACCACCAGCGCCGAACGCGGCGGATCAAACAACGTGCCACCGAGTCCCGAGAGCAGGCATGAAAACCACAATAGCCACGGt                                                                                                      >  1:54980/1‑251 (MQ=255)                                                                                                                                                   cgccgcACATAGCACAAATAGAACTGCCCCTGTGGCGCAGACCAGGCGAAAGTCGTATTGCAACAGCCAGCTCCCCAACAATGCGCCAATGACCGCCCCAGCACTGTCCTGCATCATCAACAGCGAGAAAAAACGACCA‑CGCTGCTGTGGACGGATTAATTTCACCACCAGCGCCGAACGCGGCGGATCAAACAACGTGCCACCGAGTCCCGAGAGCAGGCATGAAAACCACAATAGCCCCGGTTCGTggg                                                                                               <  1:41197/251‑1 (MQ=255)                                                                                                                                                        cacaTAGCACAAATAGAACTTCCCCTGTGGCGCAGACCAGGCGAAAGTCGTATTGCAACAGCCAGCTCCCCAACAATGCGCCAATGACCGCACCAGCACTGTCCTGCATCATCAACAGCGAGAAAAAACGACCA‑CGCTGCTGTGGACGGATTAATTTCACCACCAGCGCCGAACGCGGCGGATCAAACAACGTGCCACCGAGTCCCGAGAGCAGGCATGAAAACCACAATAGCCACg                                                                                                        >  1:291597/1‑237 (MQ=255)                                                                                                                                                           aTAGCACAAATAGAACTGCCCCTGTGGCGCAGACCAGGCGAAAGTCGTATTGCAACAGCCAGCTCCCCAACAATGCGCCAATGACCGCACCAGCACTGTCCTGCATCATCAACAGCGAGAAAAAACGACCA‑CGCTGCTGTGGACGGATTAATTTCACCACCAGCGCCGAACGCGGCGGATCAAACAACGTGCCACCGAGTCCCGAGAGCAGGCATGAAAACCACAATAGCCACGGTTCGTggccgtacccc                                                                                       >  1:41839/1‑242 (MQ=255)                                                                                                                                                            tAGCACAAATAGAACTGCCCCTGTGGCGCAGACCAGGCGAAAGTCGTATTGCAACAGCCAGCTCCCCAACAATGCGCCAATGACCGCACCAGCACTGTCCTGCATCATCAACAGCGAGAAAAAACGACCA‑CGCTGCTGTGGACGGATTAATTTCACCACCAGCGCCGAACGCGGCGGATCAAACAACGTGCCACCGAGTCCCGAGAGCAGGCATGAAAACCACAATAGCCACGGTTCGTGGGCGATAccc                                                                                       >  2:397140/1‑250 (MQ=255)                                                                                                                                                             aGCACAAATAGAACTGCCCCTGTGGCGCAGACCAGGCGAAAGCCGTATTGCAACAGCCAGCCCCCCAGCAATGCGCCAATGACCGCACCAGCACTGTCCTGCATCATCAACAGCGAGAAAAAACGACCA‑CGCTGCTGTGGACGGATTAATTTCACCACCAGCGCCGAACGCGGCGGATCAAACAACGTGCCACCGAGTCCCGAGAGCAGGCATGAAAACCACAATAGCCACGGTTCGTGGGCGATACCCa                                                                                      <  2:329802/250‑1 (MQ=255)                                                                                                                                                                       gAACTGCCCCTGTGGCGCAGACCAGGCGAAAGTCGTATTGCAACAGCCAGCTCCCCAACAATGCGCCAATGACCGCACCAGCACTGTCCTGCATCATCAACAGCGAGAAAAAACGACCA‑CGCTGCTGTGGACGGATTAATTTCACCACCAGCGCCGAACGCGGCGGATCAAACAACGTGCCACCGAGTCCCGAGAGCAGGCATGAAAACCACAATAGCCACGGTTCGTGGGCGATACCCATTGTGGCGAAt                                                                           <  2:383062/251‑1 (MQ=255)                                                                                                                                                                       gAACTGCCCCTGTGGCGCAGACCAGGCGAAAGTCGTATTGCAACAGCCAGCTCCCCAACAATGCGCCAATGACCGCACCAGCACTGTCCTGCATCATCAACAGCGAGAAAAAACGACCA‑CGCTGCTGTGGACGGATTAATTTCACCACCAGCGCCGAACGCGGCGGATCAAACAACGTGCCACCGAGTCCCGAGAGCAGGCATGAAAACCACAATAGCCACGGTTCGTGGGCGATACCCATTGTGGCGAAt                                                                           <  1:39738/251‑1 (MQ=255)                                                                                                                                                                       aaaCTGCCCCTGTGGCGCAGACCAGGCGAAAGTCGTATTGCAACAGCCAGCTCCCCAACAATGCGCCAATGACCGCCCCAGCACTGTCCTGCATCATCAACAGCGAGAAAAAACGACCA‑CGCTGCTGTGGACGGATTAATTTCACCACCAGCGCCGAACGCGGCGGATCAAACAACGTGCCACCGAGTCCCGAGAGCAGGCATGAAAACCACAATAGCCACGGTTCGTGGGCGATACCCATTGTGGCGAAt                                                                           <  2:55323/250‑1 (MQ=255)                                                                                                                                                                             ccccTGTGGCGCAGACCAGGCGAAAGTCGTATTGCAACAGCCAGCTCCCCAACAATGCGCCAATGACCGCACCAGCACTGTCCTGCATCATCAACAGCGAGAAAAAACGACCA‑CGCTGCTGTGGACGGATTAATTTCACCACCAGCGCCGAACGCGGCGGATCAAACAACGTGCCACCGAGTCCCGAGAGCAGGCATGAAAACCACAATAGCCACGGTTCGTGGGCGATACCCATTGTGGCGAATCcggcg                                                                     >  2:139233/1‑251 (MQ=255)                                                                                                                                                                                cTGTGGCGCAGACCAGGCGAAAGTCGTATTGCAACAGCCAGCTCCCCAACAATGCGCCAATGACCGCACCAGCACTGTCCTGCATCATCAACAGCGAGAAAAAACGACCA‑CGCTGCTGTGGACGGATTAATTTCACCACCAGCGCCGAACGCGGCGGATCAAACAACGTGCCACCGAGTCCCGAGAGCAGGCATGAAAACCACAATAGCCACGGTTCGTGGGCGATACCCATTGTGGCGAATCCGgcggcg                                                                  >  2:99738/1‑251 (MQ=255)                                                                                                                                                                                         aGACCAGGCGAAAGTCGTATTGCCACAGCCAGCTCCCCAACAAGGCGCCAATGACCGCACCAGCACTGTCCGGCATCATCAACAGCGAGAAAGAACGACCA‑CGCTGCTGTGGACGGATTAATTTCACGACCAGCTCCGAGCGCGGCGGATCAAACAACTTGCCACCGATGCCCGAGAGCAGGCTTGAAAACCACAAGAGCCACGGTGCGTGGGCGATACCCATGGTGGCGAATCCGGCGGCGCGCATCAGc                                                         <  1:267566/251‑1 (MQ=255)                                                                                                                                                                                             cAGGCGAAAGTCGTATTGCAACAGCCAGCTCCCCAACAATGCGCCAATGACCGCACCAGCACTGTCCTGCATCATCAACAGCGAGAAAAAACGACCA‑CGCTGCTGTGGACGGATTAATTTCACCACCAGCGCCGAACGCGGCGGATCAAACAACGTGCCACCGAGTCCCGAGAGCAGGCATGAAAACCACAATAGCCACGGTTCGTGGGCGATACCCATTGTGGCGAATCCGGCGGCGCGCATCAGCATAc                                                     <  1:99738/251‑1 (MQ=255)                                                                                                                                                                                                      gTCGTATTGCAACAGCCAGCTCCCCAACAATGCGCCAATGACCGCACCAGCACTATCCACCATCATCAACAGCGAGACAAAACGACCA‑CGCTGCTGCGGACGGATCAATTTCACCACCAGCGCAGAACGCGGCGGATCCAACAACGTGCCACCGAGTCCCGAGAGCAGGCATGAAAACCACAATAGCCACGGTTCGTGCGCGATACCCATTGTGGCGAATCCGGCGGCGCGCATCAGCATACCGGCAACa                                             >  1:288214/1‑250 (MQ=255)                                                                                                                                                                                                       tCGTATTGCAACAGCCAGCTCCCCAACAATGCGCCAATGACCGCACCAGCACTGTCCTGCATCATCAACAGCGAGAAAAAACGACCA‑CGCTGCTGTGGACGGATTAATTTCACCACCAGCGCCGAACGCGGCGGATCAAACAACGTGCCACCGAGTCCCGAGAGCAGGCATGAAAACCACAATAGCCACGGTTCGTGGGCGATACCCATTGTGGCGAATCCGGCGGCGCGCATCAGCATACCGGTAACAat                                           >  1:87300/1‑251 (MQ=255)                                                                                                                                                                                                                                                cGCACCAGAACTGTCCTGCATCACAAACATCGAGAAAAAACGCCCA‑CGTTGCGGTGGACTGATTAATTTCACCACCAGCGCCGAACGCGGCGGATCAAACAACGTGCCACCGATTCCCGAGAGCAGGCATGAAACCCCCAATAGCCACGGTTCGTGGGCGATACCCATTGTGGCGAATCCGGCGGCGCGCATCAGCATACCGGTAACAATCATCGTTTTGGCACCAAAGCGTTCGGCAATTGCACCGCCGa  <  2:312445/251‑1 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                      ATCACTTGTACAGCCAGCATGTAGTAACCCGCCAGCGTCAGAACATAGGTGACAAAACGCTTGTCACGCATCACGCGGGTCATGCCTTCGCGAACGGGCGTGCGTACGGTGGAGAGTTTCCATGCTGGTAACAACCACGCATTGAACGCCGCACATAGCACAAATAGAACTGCCCCTGTGGCGCAGACCAGGCGAAAGTCGTATTGCAACAGCCAGCTCCCCAACAATGCGCCAATGACCGCACCGGCACTGTCCTGCATCATCAACAGCGAGAAAAAACGACCA‑CGCTGCTGTGGACGGATTAATTTCACCACCAGCGCCGAACGCGGCGGATCAAACAACGTGCCACCGAGTCCCGAGAGCAGGCATGAAAACCACAATAGCCACGGTTCGTGGGCGATACCCATTGTGGCGAATCCGGCGGCGCGCATCAGCATACCGGTAACAATCATCGGTTTGGCACCAAAGCGGTCGGCAATTGCACCGCCGA  >  NC_000913/1124644‑1125133

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑