Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,258,643	G→A	N218N (AAC→AAT)	psuK ←	putative pseudouridine kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,258,643	0	G	A	100.0%	91.7 / NA	33	N218N (AAC→AAT)	psuK	putative pseudouridine kinase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (22/11);  total (22/11)

ATTCACAGGAGAGCGCCATTGACGAACATCCCTGTGCGAAACGAACAGATTCGGCAAACGGCATTCCGTCTACCCAACACGAAGCAAGTCCCGCCATCATGGCATCGCCCGCTCCGGTAACATTAATAACATTGGTTTTGATCGGCGCAGACCAGCCACTTTCACCGCTGATATCGCTGTAATAAACGCCGTCGCCGCCCATGCTCAATACCAGTCGGTTCAGGCCATGTTGATGGAACCAGGCAGCAACTTTTGCCACATCTTCACGCCCTGACAGCGCAATCCCACTCAGGGTTTCCGCTTCAAGGCGGTTTGGCTTGAGAGTGTGGATCTGATTTAGACGGTCGCGCACTTTGACACATTTCCATGCCGAAACCGGATCGACAAATACGGGTACGTTGGCGGCATTATCCAGAATCCATGCCAGTGCCTCTTCACTGATATTACAGTCCGCGACAATG  >  NC_000913/2258426‑2258886                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                    aTTCACAGGAGGGCGCCATTGACGAACATCCCTGGGCGAAACGAACAGATTCGGCAAACGGCATTCCGTCTCCCCAACACAAAGCAAGTCCCGCCATCATGGCATCGCCCGCTCCGGTAACATTAATAACATTGGTTTTGATCGGCGCAGACCAGCCACTTTCACCGCTGATATCGCTGTAATAAACGCCGTCGCCGCCCATGCTCAATACCAGTCGATTCAGGCCATGTTGATGGAACCAGGCAGCAACt                                                                                                                                                                                                                    <  2:431760/251‑1 (MQ=255)                     gACGAACATCCCTGTGCGAAACGAACAGATTCGGCAAACGGCATTCCGTCTACCCAACACGAAGCAAGTCCCGCCATCATGGCATCGCCCGCTCCGGTAACATTAATAACATTGGTTTTGATCGGCGCAGACCAGCCACTTTCACCGCTGATATCGCTGTAATAAACGCCGTCGCCGCCCATGCTCAATACCAGTCGATTCAGGCCATGTTGATGGAACCAGGCAGCAACTTTTGCCACATCTTCACGccc                                                                                                                                                                                                >  1:417556/1‑251 (MQ=255)                      aCGAACATCACTGCGCGAAACGAACAGATTCGGCAAACGGCATTCCGTCTACCCAACAAGAAGCAAGTCCCGCCATCATGGCATCGCCCGCTCCGGTAACATTACTAACATTGGTTTTGATCGGCGAAGACCAGCCACTTTCACCGCTGATCTCGCTGTAATAAACACCGTAGCCGCCCATGCTCAATACCAGTCGATTCAGGCCATGTTGATGGAACCAGGCAGAAACTTTTGCCCCATCTTAACGCCCt                                                                                                                                                                                               >  2:250860/1‑251 (MQ=255)                        gAACATCCCAGTGCGATCCGAACAGATTCGGCAAACGGCATTCCGTCTACCCAACACGAAGCAAGTCCCGCCATCACGGCATCGCCCGCTCCGGTAACATTAATAACATTGGTTTTGATCGGCGCAGACACGCCACTTTCACCGCTGATATCGCTGTAATAAACGCCGTCGCCGCCCATGCTCAATAACAGTCGATTCAGGCCATGTTGATCGAACCAGGAAGCACCTCTTGCCACATCTTCACGCTCTgc                                                                                                                                                                                             >  2:451360/1‑250 (MQ=255)                         aaCATCCCTGTGCGAAACGAACAGATTCGGCAAACGGCATTCCGTCTACCCAACACGAAGCAAGTCCCGCCATCATGGCATCGCCCGCTCCGGTAACATTAATAACATTGGTTTTGATCGGCGCAGACCAGCCACTTTCACCGCTGATATCGCTGTAATAAACGCCGTCGCCGCCCATGCTCAATACCAGTCGATTCAGGCCATGTTGATGGAACCAGGCAGCAACTTTTGCCACATCTTCACGCCCTGAc                                                                                                                                                                                            >  1:41397/1‑251 (MQ=255)                             tCCCTGTGCGAAACGAACAGATTCGGCAAACGGCATTCCGTCTACCCAACACGAAGCAAGTCCCGCCATCATGGCATCGCCCGCTCCGGTAACATTAATAACATTGGTTTTGATCGGCGCAGACCAGCCACTTTCACCGCTGATATCGCTGTAATAAACGCCGTCGCCGCCCATGCTCAATACCAGTCGATTCAGGCCATGTTGATGGAACCAGGCAGCAACTTTTGCCACATCTTCACGCCCTGACAgcg                                                                                                                                                                                        >  1:45456/1‑251 (MQ=255)                                         aCGAACAGATTCGGCAAACGGCATTCCGTCTACCCAACACGAAGCAAGTCCCGCCATCATGGCATCGCCCGCTCCGGTAACATTAATAACATTGGTTTTGATCGGCGCAGACCAGCCACTTTCACCGCTGATATCGCTGTAATAAACGCCGTCGCCGCCCATGCTCAATACCAGTCGATTCAGGCCATGTTGATGGAACCAGGCAGCAACTTTTGCCACATCTTCACGCCCTGACAGCGCAATCCCACTc                                                                                                                                                                             >  2:202739/1‑250 (MQ=255)                                                     aggaaaCGGCATTCCGTCTACACAACACGAAGCAAGTCCCGCCATCATGGCATCGCCCGCTCCGGTAACATTAATAACATTGGTTTTGATCGGAGCAGACCAGCCACTTTCACCGCTGATATCGCTGTAATAAACGCCGTCGCCGCCCATGCTCAATACCAGTCGATTCAGGCCATGTTGATGGAACCAGGAAGCAAATTTTGCTACATCTTCACGCCCTTACAGCGCAATCCCACTCAGGGTTTCAGCtt                                                                                                                                                                >  2:295680/4‑251 (MQ=255)                                                       cAAACGGCATTCCGTCTACCCAACACGAAGCAAGTCCCGCCATCATGGCATCGCCCGCTCCGGTAACATTAATAACATTGGTTTTGATCGGCGCAGACCAGCCACTTTCACCGCTGATATCGCTGTAATAAACGCCGTCGCCGCCCATGCTCAATACCAGTCGATTCAGGCCATGTTGATGGAACCAGGCAGCAACTTTTGCAACATCTTCACGCCCTGACAGCGCAATCCCACTCAGGGTTTCCGCTTc                                                                                                                                                               >  2:82524/1‑250 (MQ=255)                                                        agaCGGCATTCCGTCTACCCAACACGAAGCAAGTCCCGCCATCATGGCATCGCCCGCTCCGGTAACATTAATAACATTGGTTTTGATCGGCGCAGACCAGCCACTTTCACCGCTGATATCGCTGTAATAAACGCCGTCGCCGCCCATGCTCAATACCAGTCGATTCAGGCCATGTTGATGGAACCAGGCAGCAACTTTTGCCACATCTTCACGCCCTGACAGCGCAATCCCACTCAGGGTTTCCGCTTCa                                                                                                                                                              >  1:308265/3‑250 (MQ=255)                                                        aaaCGGCATTCCGTCTACCCAACACGAAGCAAGTCCCGCCATCATGGCATCGCCCGCTCCGGTAACATTAATAACATTGGTTTTGATCGGCGCAGACCAGCAACTTTCACCGCTGATATCGCTGTAATAAACGCCGTCGCCGCCCATGCTCAATACCAGTCGATTCAGGCCATGTTGATGGAACCAGGCAGCAACTTTTGCCACATCTTCACGCCCTGACAGCGCAATCCCACTCAGGGTTTCCGCTTCa                                                                                                                                                              >  1:308277/1‑250 (MQ=255)                                                         aagggctttccaccaccccAAAACGAAGAAAGTCCCGCCCTCAGGGCATCGCCCGCTCCGGTAACATTAATAACATTGGTTTTTATCGGCTCAGACCAGCCACTTTCACCGCTGATATCGCTGTAATAAACGCCGTCGCCGCCCATGCTCAATACCAGTCGATTCAGGCCATGTTGATGGAACCAGGCAGCAACTTTTGCCACATCTTCCCGCCCTGACAGCGCAATCCCACTCAGGGTTTCCGCTTCAAg                                                                                                                                                            <  2:284921/235‑1 (MQ=255)                                                                                   aGCAAGTCCCGCCATCATGGCATCGCCCGCTCCGGTAACATTAATAACATTGGTTTTGATCGGCGCAGACCAGCCACTTTCACCGCTGATATCGCTGTAATAAACGCCGTCGCCGCCCATGCTCAATACCAGTCGATTCAGGCCATGTTGATGGAACCAGGCAGCAACTTTTGCCACATCTTCACGCCCTGACAGCGCAATCCCACTCAGGGTTTCCGCTTCAAGGCGGCTTGGCTTGAGAGTGTGGAtat                                                                                                                                  >  1:313623/1‑249 (MQ=255)                                                                                             gCCATCATGTCATCGCCCCCTCCGGTATCATTAATAACATTTGTTTTGATCGGCGTAGACCAGCCGCTTTCACCGCTGATATCGCTGTAATAAACGCCGTCGCCGCCCATGCTCAATACCAGTCGATTCAGGCCATGTTGATGGAACCAGGCAGCAACTTTTGCCACATCTTCACGCCCTGACAGCGCAATCCCACTCAGGGTTTCCGCTTCAAGGCGGTTTGGCTTGAGAGTGTGGATCTGATTTAgccg                                                                                                                        <  1:260860/251‑4 (MQ=255)                                                                                                          cGCCCGCTCCGGTAACATTAATAACATTGGTTTTGATCGGCGCAGACCAGCCACTTTCACCGCTGATATCGCTGTAATAAACGCCGTCGCCGCCCATGCTCAATACCAGTCGATTCAGGCCATGTTGATGGAACCAGGCAGCAACTTTTGCCACATCTTCACGCCCTGACAGCGCAATCCCACTCAGGGTTTCCGCTTCAAGGCGGTTTGGCTTGAGAGTGTGGATCTGATTTAGACGGTCGCGCACttt                                                                                                            <  1:32526/250‑1 (MQ=255)                                                                                                           gCCCGCTCCGGAAACATTAATAACATTGGTTTTGATCGGCGCAGACCCGCCACTTTCACCGCTGATATCGCTGTAATAAACGCCGGCGCCGCCCATGCTCAATACCAGTCGATTCAGGCCATGTTGATGGAACCAGGCAGCAACTTTTGCCACATCTTCACGCCCTGACAGCGCAATCCCACTCAGGGTTTCCGCTTCAAGGCGGTTTGGCTTGAGAGTGTGGATCTGCTTTAGACGGTCTAGCACTTTg                                                                                                           >  1:157237/1‑250 (MQ=255)                                                                                                                  ccGGTAACATTAAAAACATTGGTTTTGATCGGCGCAGACCAGCCACTTTCACCGCTGATATCGCTGTAATAAACGCCGTCGCCGCCCATGCTCAATACCAGTCGATTCAGGCCATGTTGATGGAACCAGGCAGCAACTTTTGCCACATCTTCACGCCCTGACAGCGCAATCCCACTCAGGGTTTCCGCTTCAAGGCGGTTTGGCTTGAGAGTGTGGATCTGATTTAGACGGTCGCGCACTTTGACACAttt                                                                                                   <  2:21316/251‑1 (MQ=255)                                                                                                                       aaCATTAATAACATTGGTTTTGATCGGCGCAGACCAGCCACTTTCACCGCTGATATCGCTGTAATAAACGCCGTCGCCGCCCATGCTCAATACCAGTCGATTCAGGCCATGTTGATGGAACCAGGCAGCAACTTTTGCCACATCTTCACGCCCTGACAGCGCAATCCCACTCAGGGTTTCCGCTTCAAGGCGGTTTGGCTTGAGAGTGTGGATCTGATTTAGACGGTCGCGCACTTTGACACATTTCCATg                                                                                              >  2:435948/1‑251 (MQ=255)                                                                                                                                     gtgttgtaagaggcgcgaaCCAGCCACTTTCACCGCTTATATCGCTGTAATAAACGCCGTCGCCGCACATGCTCAATACCAGTCGATTCAGGCCATGTTGATGGAACCAGGCAGCAACTTTTGCCACATCTTCACGCCCTGACAGCGCAATCCCACTCAGGGTTTCCGCTTCAAGGCGGTTTTGCTTGAGAGTGTGGATCTGATTTAGACGGTCGCGCACTTTGACACATTTCCATGCCGAAACCGGATc                                                                                 <  2:206438/232‑1 (MQ=255)                                                                                                                                        ttttGATCGGCGCAGACCAGCCACTTTCACCGCTGATATCGCTGTAATAAACGCCGTCGCCGCCCATGCTCAATACAAGTCGATTCAGGCCATGTTGATGGAACAAGGCAGCAACTTTTGCAACATCTTCACGCCCTGACAGCGCAATCCCACTCAGGGTTTCCGCTTCAAGGCGGTTTGGCTTGAGAGTGTGGATCTGATTTAGACGGTCGCGCACATTGACACATTTCAATGCCGAAACCGGATAGAc                                                                              >  1:409722/1‑250 (MQ=255)                                                                                                                                          ttGATCGGCGCAGACCAGCCACTTTCACCGCTGATATCGGTGAAATAAACGCCGTCGCCGCCCATGCTCAATACCAGTCGATTCAGGCCATGTTGATGGAACCAGGCAGCAACTTTTGCCACATCTTCACGCCCTGACAGCGCAATCCCACTCAGGGTTTACGCTTCAAGGCGGTTTGGCTTGAGAGTGTGGATCTGAt                                                                                                                               >  1:173193/1‑199 (MQ=255)                                                                                                                                          ttGATCGGCGCAGACCAGCCACTTTCACCGCTGATATCGCTGTAATAAACGCCGTCGCCGCCCATGCTCAATACCAGTCGATTCAGGCCATGTTGATGGAACCAGGCAGCAACTTTTGCCACATCTTCACGCCCTGACAGCGCAATCCCACTCATGGTTTCCGCTTCAAGGCGGTTTGGCTTGAGAGTGTGGATCTGAt                                                                                                                               <  2:173193/199‑1 (MQ=255)                                                                                                                                                        ccAGCCACTTTCACCGCTGATATCGCTGTAATAAACGCCGTCGCCGCCCATGCTCAATACCAGTCGATTCAGGCCATGTTGATGGAACCAGGCAGCAACTTTTGCCACATCTTCACGCCCTGACAGCGCAATCCCACTCAGGGTTTCCGCTTCAAGGCGGTTTGGCTTGAGAGTGTGGATCTGATTTAGACGGTCGCGCACTTTGACACATTTCCATGCCGAAACCGGATCGACAAATACGGGTACGTTgg                                                             >  1:112081/1‑251 (MQ=255)                                                                                                                                                         cAGCCACTTTCACCGCTGATATCGCTGTAATAAACGCCGTCGCCGCCCATGCTCAATACCAGTCGATTCAGGCCATGTTGATGGAACCAGGCAGCAACTTTTGCCACATCTTCACGCCCTGACAGCGCAATCCCACTCAGGGTTTCCGCTTCAAGGCGGTTTGGCTTGAGAGTGTGGATCTGATTTAGACGGTCGCGCACTTTGACACATTTCCATGCCGAAACCGGATCGACAAATACgggga                                                                   >  2:369889/1‑242 (MQ=255)                                                                                                                                                         cAGCCACTTTCACCGCTGATATCGCTGTAATAAACGCCGTCGCCGCCCATGCTCAATACCAGTCGATTCAGGCCATGTTGATGGAACCAGGCAGCAACTTTTGCCACATCTTCACGCCCTGACAGCGCAATCCCACTCAGGGTTTCCGCTTCAAGGCGGTTTGGCTTGAGAGTGTGGATCTGATTTAGACGGTCGCGCACTTTGACACATTTCCATGCCGAAACCGGATCGACAAATACGGGTa                                                                   <  1:369889/244‑1 (MQ=255)                                                                                                                                                          aGCCACTTTCACCGCTGATATCGCTGTAATAAACGCCGTCGCCGCCCATGCTCAATACCAGTCGATTCAGGCCATGTTGATGGAACCAGGCAGCAACTTTTGCCACATCTTCACGCCCTGACAGCGCAATCCCACTCAGGGTTTCCGCTTCAAGGCGGTTTGGCTTGAGAGTGTGGGTCTGATTTATACGGTCGCGCACTTTGACACATTTCTATTCCCAAACCGGATCGACAAATACGGGTAAGTTgtcg                                                           >  2:363856/1‑248 (MQ=255)                                                                                                                                                                   cACCGCTGATATCGCTGTAATAAACGCCGTCGCCGCCCATGCTCAATACCAGTCGATTCAGGCCATGTTGATGGAACCAGGCAGCAACTTTTGCCACATCTTCACGCCCTGACAGCGCAATCCCACTCAGGGTTTCCGCTTCAAGGCTGTTTGGCTTGAGAGTGTGGATCTGATTTAGACGGTCGCGCACTTTGCCACATTTCCATGCCGAAACCGGATCGACAAATACGGGTACGTTGGCGGCATTATc                                                   <  1:405934/250‑1 (MQ=255)                                                                                                                                                                     ccGCTGATATCGCTGTAATAAACGCCGTCGCCGCCCATGCTCAATACCAGTCGATTCAGGCCATGTTGATGGAACCAGGCAGCAACTTTTGCCACATCTTCACGCCCTGACAGCGCAATCCCACTCAGGGTTTCCGCTTCAAGGCGGTTTGGCTTGAGAGTGTGGATCTGATTTAAACGGTCGCGCACTTTGACACATTTCCATGCCGAAACCGGATCGACAAATACGGGTACGTTGGCGGCATTATcc                                                  >  2:430173/1‑249 (MQ=255)                                                                                                                                                                                        aaaCGCCGTCGCCGCCCATGCTCAATACCAGTCGATTCAGGCCATGTTGATGGAACCAGGCAGCAACTTTTTCCACATCTTCACGCCCTGACAGCGCAATCCCACACAGGGTTTCCGCTTCAAGGCGGTTTGGCTTGAGAGTGTGGATCTGATTTAGACGGTCGCGCACTTTGACACATTTCCATGCCGAAACCGGATCGACAAATACCGGTACGTTGGCGGCATTATCCAGAATCCATGCCAGCGCctc                              <  2:313623/250‑1 (MQ=255)                                                                                                                                                                                          aCGCCGTCGCCGCCCATGCTCAATACCAGTCGATTCAGGCCATGTTGATGGAACCAGGCAGCAACTTTTGCCACATCTTCACGCCCTGACAGCGCAATCCCACTCAGGGTTTCCGCTTCAAGGCGGTTTGGCTTGAGAGTGTGGATCTGATTTAGACGGTCGCGCACGTTGACACATTTCCATGCCGAAACCGGATCGACAAATACGGGTACGTTGGCGGCATTATCCAGAATCCATGCCAGTGCCTCTTc                           <  2:45456/251‑1 (MQ=255)                                                                                                                                                                                            gcagTCGCCGCCCATGCTCAATACCAGCCGATTCATGCCATGTTGATGGAACCAGGCAGCAACTTTTGCCACATCTTCACGCCCTGACAGCGCAATCCCACTCAGGGTTTCCGCTTCAAGGCGGTTTGGTTTGAGAGGGTGGATCTGATTTAGACGGTGGCGCACTTTGACCCATTTCCCTGCCCAAACCGGATCGACAAATACGGGGACGGTGGCGGGATTTTCCAGAATCCATGCCAGCGCCtcatca                          >  2:234942/4‑250 (MQ=255)                                                                                                                                                                                                               aaTACCAGTCGATTCAGGCCATGTTGATGGAACCAGGCAGCAACTTTTGCCACATCTTCACGCCCTGACAGCGCAATCCCACTCAGGGTTTCCGCTTCAAGGCGGTTTGGCTTGAGAGTGTGGATCTGATTTAGACGGTCGCGCACTTTGACACATTTCCATGCCGAAACCGGATCGACAAATACGGGTACGTTGGCGGCATTATCCAGAATCCATGCCAGTGCCTCTTCACTGATATTACAGTCCGCGAc      >  2:55207/1‑251 (MQ=255)                                                                                                                                                                                                                    cAGTCGATTCAGGCCATGTTGATGGAACCAGGCAGCAACTTTTGCCACATCTTCACGCCCTGACAGCGCAATCCCACTCAGGGTTTCCGCTTCAAGGCGGTTTGGCTTGAGAGTGTGGATCTGATTTAGACGGTCGCGCACTTTGACACATTTCCATGCCGAAACCGGATCGACAAAAACGGGTACGTTGGCGGCATTATCCAGAATCCATGCCAGGGCCTCTTCACTGATATTACAATCCGCGACAATg  >  1:401461/1‑250 (MQ=255)                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                    ATTCACAGGAGAGCGCCATTGACGAACATCCCTGTGCGAAACGAACAGATTCGGCAAACGGCATTCCGTCTACCCAACACGAAGCAAGTCCCGCCATCATGGCATCGCCCGCTCCGGTAACATTAATAACATTGGTTTTGATCGGCGCAGACCAGCCACTTTCACCGCTGATATCGCTGTAATAAACGCCGTCGCCGCCCATGCTCAATACCAGTCGGTTCAGGCCATGTTGATGGAACCAGGCAGCAACTTTTGCCACATCTTCACGCCCTGACAGCGCAATCCCACTCAGGGTTTCCGCTTCAAGGCGGTTTGGCTTGAGAGTGTGGATCTGATTTAGACGGTCGCGCACTTTGACACATTTCCATGCCGAAACCGGATCGACAAATACGGGTACGTTGGCGGCATTATCCAGAATCCATGCCAGTGCCTCTTCACTGATATTACAGTCCGCGACAATG  >  NC_000913/2258426‑2258886

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑