Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,812,084	G→A	A159V (GCG→GTG)	yicR ←	RadC‑like JAB domain‑containing protein YicR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,812,084	0	G	A	96.3%	81.9 / NA	28	A159V (GCG→GTG)	yicR	RadC‑like JAB domain‑containing protein YicR
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base A (11/15);  total (11/17)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.55e-01

CGATGTGCTCAAGTCCCGAACAGACAAAGATCCCGAAGGATCGCGCATAGCGGGTTAAATCCAGCCGCGTTCGGCAAAAGAAACATACTCTCCACGCCCAATCACGATATGGTCGAGCACGCGTAAATCCATGAACTGACAACTCTTTATTATCCGTTCAGTAATGAGTTTATCCGCTTTACTGGGTTCAGCACAACCCGAAGGGTGATTATGTGCAAGGATCAGCGCCGAGGCGTTTATTTTTATCGCTTCGCGGATAATTTCCCGAGGATGGACTTCAACATGGTTTAGCGTGCCGGAAAAAAGACGCCTATGCGTTATAACCCGGTGTTGGGAGTCGAGAAAGATCACCATAAAGATCTCCCGCTCCTCACCCGTGAGCTGGCTTTGTAAAAATTCCCTCGTCATCTCCGGGCTGAGTAAAGGGCTTTCTTCACGCATCCGCACGTTGTAGTAACGCCGCGCCAGTT  >  NC_000913/3811837‑3812306                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                               cGATGTGCTTGAGTCACGAACAGACAAAGATCCGGAAGGATCGCGCATATCGGGTTAATGCCAGCCGCGTTCGGCAAAATAAACATACTCTCCCCTGCCAATCTCGATATGGTCGAGCACGCGTAAATCCATGATCTGACAACTCTTGATTATCCGTTCAGTTATGAGTTTATCCGCTTTACTGGGTTCAGCACAACCCGAAGTGTGATTATGTGCAAGGAGCAGCGCCGAGTCTTTTATTTTTAGCGCtt                                                                                                                                                                                                                             <  2:31272/251‑1 (MQ=255)           aaGTCCCGAACAGACAAAGATCCCGAAGGATCGCGCATAGCGGGTTAAATCCAGCCGCGTTCGGCAAAAGAAACATACTCTCCACGCCCAATCACGATATGGTCGAGCACGCGTAAATCCATGAACTGACAACTCTTTATTATCCGTTCAGTAATGAGTTTATCCGCTTTACTGTGTTCAGCACAACCCGAAGGGTGATTATGTGCAAGGATCAGCGCCGAGGCGTTTATTTTTATCACTTCGCGGATAAt                                                                                                                                                                                                                   <  2:440577/251‑1 (MQ=255)               cccGAACAGACAAAGATCCCGAAGGATCGCGCATAGCGGGTTAAATCCAGCCGCGTTCGGCAAAAGAAACATACTCTCCACGCCCAATCACGATATGGTCGAGCACGCGTAAATCCATGAACTGACAACTCTTTATTATCCGTTCAGTAATGAGTTTATCCGCTTTACTGGGTTCAGCACAACCCGAAGGGTGATTATGTGCAAGGATCAGCGCCGAGGCGTTTATTTTTATCACTTCGCGGATAATTTcc                                                                                                                                                                                                               >  1:443843/1‑251 (MQ=255)                  gAACAGACAAAGATCCCGAAGGATCGCGCATAGCGGGTTAAATCCAGCCGCGTTCGGCAAAAGAAACATACTCTCCACGCCCAATCACGATATGGTCGAGCACGCGTAAATCCATGAACTGACAACTCTTTATTATCCGTTCAGTAATGAGTTTATCCGCTTTACTGGGTTCAGCACAACCCGAAGGGTGATTATGTGCAAGGATCAGCGCCGAGGCGTTTATTTTTATCACTTCGCGGATAATTTCCCg                                                                                                                                                                                                             >  2:87468/1‑250 (MQ=255)                                    aaGGATCGCGCATAGCGGGTTAAATCCAGCCGCGTTCGGCAAAAGAAACATACTCTCCACGCCCAATCACGATATGGTCGAGCACGCGTAAATCCATGAACTGACAACTCTTTATTATCCGTTCAGTAATGAGTTTATCCGCTTTACTGGGTTCAGCACAACCCGAAGGGTGATTATGTGCAAGGATCAGCGCCGAGGCGTTTATTTTTATCACTTCGCGGATAATTTCCCGAGGATGGACTTCAACATgg                                                                                                                                                                                          <  1:87468/251‑1 (MQ=255)                                              cATAGCGGGTTAAATCCAGCCGCGTTCGGCAAAAGAAACATACTCTCCACGCCCAATCACGATATGGTCGAGCACGCGTAAATCCATGAACTGACAACTCTTTATTATCCGTTCAGTAATGAGTTTATCCGCTTTACTGGGTTCAGCACAACCCGAAGGGTGATTATGTGCAAGGATCAGCGCCGAGGCGTTTATTTTTATCACTTCGCGGATAATTTCCCGAGGATGGACTTCAACATGGTTTAGCGTGc                                                                                                                                                                                <  1:457188/251‑1 (MQ=255)                                                                                  aaCATACTCTCCACGCCCAATCACGATATGGTCGAGCACGCGTAAATCCATGAACTGACAACTCTTTATTATCCGTTCAGTAATGAGTTTATCCGCGTTACTGGGTTCAGCACAACCCGAAGGGTGATTATGTGCAAGGATCAGCGCCGAGGCGTTTATTTTTATCACTTCGCGGATAATTTCCCGAGGATTGACTTCAACATGGTTTAGCGTGCCGGAAAAAAGACGCCTATGCGTTATAACCCGGTGtt                                                                                                                                            <  2:154474/251‑1 (MQ=255)                                                                                      tACTCTCCACGCCCAATCACGATATGGACGAGCACGCGTAAATCAATGAACTGACAACTCTTTATTATCCGTTCAGTAATGAGTTTATCCGCTTTACTGGGTTCAGCACAACACGAAGGGTGATTATATGCAAGGATCAGCGCCGAGGCGTTTATTTTTATCAATTCGCGGATACTTTCCCGAGGATGGACTTCAACATGGTTTAGCGTGCCGGAAAAAAGACGCCTATGCGTTATAACCCGGTGTTggg                                                                                                                                         >  2:174225/1‑250 (MQ=255)                                                                                         cctcCACGCGCAATCACGATATGGTCGAGCACGCGTAAATACATGAACTGACAACTCTTTATTATCCGTTCAGTAATGAGTTTATCCGCGTTACTGGGTTCAGCACAACCCGAAGGGTGATTATGTGCAAGGATCAGCGCCGAGTCGTTTATTTTTATCACTTCGCGGATAATTTCCCGAGGATGGACTTCAACATGGTTTAGCGTGCCGGAAAAAAGACGCCTATGCGTGATAACCCGGTGTTGGGAGTc                                                                                                                                     <  2:430131/250‑1 (MQ=255)                                                                                                  ccAATCACGATATGGTCGAGCACGCGTAAATCCATGAACTGACAACTCTTTATTATCCGTTCAGTAATGAGTTTATCCGCTTTACTGGGTTCAGCACAACCCGAAGGGTGATTATGTGCAAGGATCAGCGCCGAGGCGTTTATTTTTATCACTTCGCGGATAATTTCCCGAGGATGGACTTCAACATGGTTTAGCGTGCCGGAAAAAAGACGCCTATGCGTTATAACCCGGTGTTGGGAGTCGAGaa                                                                                                                                >  1:104822/1‑247 (MQ=255)                                                                                                     aTCACGATATGGTCGAGCACGCGTAAATCCATGAACTGACAACTCTTTATTATCCGTTCAGTAATGAGTTTATCCGCTTTACTGGGTTCAGCACAACCCGAAGGGTGATTATGTGCAAGGATCAGCGCCGAGGCGTTTATTTTTATCACTTCGCGGATAATTTCCCGAGGATGGACTTCAACATGGTTTAGCGTGCCGGAAAAAAGACGCCTATGCGTTATAACCCGGTGTTGGGAGTCGAGAAAGATCAc                                                                                                                         >  2:305338/1‑251 (MQ=255)                                                                                                               ggTCGAGCACGCGTAAATCCATGAACTGACAACTCTTTATTATCCGTTCAGTAATGAGTTTATCCGCTTTACTGGGTTCAGCACAACCCGAAGGGTGATTATGTGCAAGGATCAGCGCCGAGGCGTTTATTTTTATCACTTCGCGGATAATTTCCCGAGGATGGACTTCAACATGGTTTAGCGTGCCGGAAAAAAGACGCCTATGCGTTATAACCCGGTGTTGGGAGTCGAGAAAGATCACCATaa                                                                                                                    >  1:213214/1‑246 (MQ=255)                                                                                                                         gcgTAAATCCATGAACTGACAACTCTTTATTATCCGTTCAGTAATGAGTTTATCCGCTTTACTGGGTTCAGCACAACCCGAAGGGTGATTATGTGCAAGGATCAGCGCCGAGGCGTTTATTTTTATCACTTCGCGGATAATTTCCCGAGGATGGACTTCAACATGGTTTAGCGTGCCGGAAAAAAGACGCCTATGCGTTATAACCCGGTGTTGGTAGTCGAGAAAGATCACCATAAAGATCTCCCGctcct                                                                                                     <  2:395466/251‑1 (MQ=255)                                                                                                                           gTAAATCCATGAACTGACAACTCTTTATTATCCGTTCAGTAATGAGTTTATCCGCTTTACTGGGTTCAGCACAACCCGAAGGGTGATTATGTGCAAGGATCAGCGCCGAGGCGTTTATTTTTATCACTTCGCGGATAATTTCCCGAGGATGGACTTCAACATGGTTTAGCGTGCCGGAAAAAAGACGCCTATGCGTTATAACCCGGTGTTGGGAGTCGAGAAAGATCACCATAAAGATCTCCCGCTCCTCa                                                                                                   <  2:104822/251‑1 (MQ=255)                                                                                                                                             aaCTCTTTATTATCCGTTCAGTAATGAGTTTATCCGCTTTACTGGGTTCAGCACAACCCGAAGGGTGATTATGTGCAAGGATCAGCGCCGAGGCGTTTATTTTTATCACTTCGCGGATAATTTCCCGAGGATGGACTTCAACATGGTTTAGCGTGCCGGAAAAAAGACGCCTATGCGTTATAACCCGGTGTTGGGAGTCGAGAAAGATCACCATAAAGATCTCCCGCTCCTCACCCGTGAGCTGGCTTTGt                                                                                 >  1:176675/1‑251 (MQ=255)                                                                                                                                                     attatCCGTTCAGTAATGAGTTTATCCGCTTTACTGGGTTCAGCACAACCCGAAGGGTGATTATGTGCAAGGATCAGCGCCGAGGCGTTTATTTTTATCACTTCGCGGATAATTTCCCGAGGATGGACTTCAACATGGTTTAGCGTGCCGGAAAAAAGACGCCTATGCGTTATAACCCGGTGTTGGGAGTCGAGAAAGATCACCATAAAGATCTCCCGCTCCTCACCCGTGAGCTGGCTTTGTAAAAATTc                                                                         <  1:421476/251‑1 (MQ=255)                                                                                                                                                        atCCGTTCAGTAATGAGTTTATCCGCTTTACTGGGTTCAGCACAACCCGAAGGGTGATTATGTGCAAGGATCAGCGCCGAGGCGTTTATTTTTATCACTTCGCGGATAATTTCCCGAGGATGGACTTCAACATGGTTTAGCGTGCCGGAAAAAAGACGCCTATGCGTTATAACCCGGTGTTGGGAGTCGAGAAAGATCACCATAAAGATCTCCCGCTCCTCACCCGTGAGCTGGCTTTGTAAAAATTCCCt                                                                      <  2:213214/251‑1 (MQ=255)                                                                                                                                                                aGTAATGAGTTTATCCGCTTTACTGGGTTCAGCACAACCCGAAGGGTGATTATGTGCAAGGATCAGCGCCGAGGCGTTTATTTTTATCACTTCGCGGATAATTTCCCGAGGATGGACTTCAACATGGt                                                                                                                                                                                         >  2:290006/1‑128 (MQ=255)                                                                                                                                                                aGTAATGAGTTTATCCGCTTTACTGGGTTCAGCACAACCCGAAGGGTGATTATGTGCAAGGATCAGCGCCGAGGCGTTTATTTTTATCACTTCGCGGATAATTTCCCGAGGATGGACTTCAACATGGt                                                                                                                                                                                         <  1:290006/128‑1 (MQ=255)                                                                                                                                                                   aaTGAGTTTATCCGCTTTACTGGGTTCAGCACAACCCGAAGGGTGATTATGTGCAAGGATCAGCGCCGAGGCGTTTATTTTTATCACTTCGCGGATAATTTCCCGAGGATGGACTTCAACATGGTTTAGCGTGCCGGAAAAAAGACGCCTATGCGTTATAACCCGGTGTTGGGAGTCGAGAAAGATCACCATAAAGAGCTCCCGCTCCTCACCCGTGAGCTGGCTTTGTAAAAATTCCCTCGTCATCTcc                                                            <  1:304493/250‑1 (MQ=255)                                                                                                                                                                          ttATCCGCTTTACTGGGTTCCGCACCACCCGAAGTGTGATTATGTGCCAGGATCAGCGCCGAGGCGTTTATTTTTATCCCTTCGCGGATCATTTCCCGATGATGGACTTCAACATTGTTTAGCGTGTCGGAAAAAAGACGCCTATGCGTTATACCCCGGTGTTGGGAGTCGAGAAAGATCACCATAAAGATCTCCCGCTCCTCACCCGTGAGCTGGCTTTGTAAAAATGCCCTCGTCATCTCCGGGCTGa                                                     <  1:145137/250‑1 (MQ=255)                                                                                                                                                                                                     cccGAAGGGTGATTATGTGCAAGGATCAGCGCCGAGGCGTTTATTTTTATCACTTCGCGGATAATTTCCCGAGGATGGACTTCAACATGGTTTAGCGTGCCGGAAAAAAGACGCCTATGCGTTATAACCCGGTGTTGGGAGTCGAGAAAGATCACCATAAAGATCTCCCGCTCCTCACCCGTGAGCTGGCTTTGTAAAAATTCCCTCGTCATCTCCGGGCTGAGTAAAGGGCTTTCTTCACGCATCCGCAc                         <  1:305338/251‑1 (MQ=255)                                                                                                                                                                                                     cccGAAGGGTGATTATGTGCAAGGATCAGCGCCGAGGCGTTTATTTTTATCACTTCGCGGATAATTTCCCGAGGATGGACTTCAACATGGTTTAGCGTGCCGGAAAAAAGACGCCTATGCGTTATAACCAGGTGTTGGGAGTCGAGAAAGATCACCATAAAGATCTCCCGCTCCTCACCCGTGAGCTGGCTTTGTAAAAATTCCCTCGTCATCTCCGGGCTGAGTAAAGGGCTTTCTTCACGCATCCGCAc                         >  1:347624/1‑251 (MQ=255)                                                                                                                                                                                                      ccGAAGGGTGATTATGTGCAAGGATCAGCGCCGAGGCGTTTATTTTTATCACTTCGCGGATAATTTCCCGAGGATGGACTTCAACATGGTTTAGCGTGCCGGAAAAAAGACGCCTATGCGTTATAACCCGGTGTTGGGAGTCGAGAAAGATCACCATAAAGATCTCCCGCTCCTCACCCGTGAGCTGGCTTTGTAAAAATTCCCTCGTCATCTCCGGGCTGAGTAAAGGGCTGTCTTCACGCATCCGCACg                        <  1:120142/251‑1 (MQ=255)                                                                                                                                                                                                      ccGAAGGGTGATTATGTGCAAGGATCAGCGCCGAGGCGTTTATTTTTATCACTTCGCGGATAATTTCCCGAGGATGGACTTCAACATGGTTTAGCGTGCCGGAAAAAAGACGCCTATGCGTTATAACCCGGTGTTGGGAGTCGAGAAAGAGCACCATAAAGATCTCCCGCTCCTCACCCGTGAGCTGGCTTTGTAAAAAGTCCCTCGTCATCTCCGGGCTGAGTAAAGGGCTTTCTTCACGCATCCGCACg                        <  2:443843/251‑1 (MQ=255)                                                                                                                                                                                                           gggTGATTATGTGCAAGGATCAGCTCCGAGGCGTTTATTTTTATCACTTCGCGGATAATTTCCCTAGGATGGACTTCAACATGGTTTAGCTTGCCGGAAAAAAGACGCCTATGCGTTATAACCCGGTGTTTGGAGTCGAGAAAGATCACCATAAAGATCTCCCGCTCCTCACCCGTGAGCTGGCTTTGTAAAAATTCCCCCGTTATCTCCTGGCTGAGTAAAGGGCTTTCTTCACGCATCCGCACGTTgta                   <  1:174225/251‑1 (MQ=255)                                                                                                                                                                                                                          aGGATCAGCGCCGAGGCGTTTATTTTTATCACTTCGCGGATAATTTCCCGAGGATGGACTTCAACATGGTTTAGCGTGCCGGAAAAAAGACGCCTATGCGTTATAACCCGGTGTTGGGAGTCGAGAAAGATCACCATAAAGATCTCCCGCTCCTCACCCGTGAGCTGGCTTTGTAAAAATTCCCTCGTCATCTCCGGGCTGAGTAAAGGGCTTTCTTCACGCATCCGCACGTTGTAGTAACGCCGCGcc      >  1:134120/1‑249 (MQ=255)                                                                                                                                                                                                                            gATCAGCGCCGAGGCGTTTATTTTTATCACTTCGCGGATAATTTCCCGAGGATGGACTTCAACATGGTTTAGCGTGCCGGAAAAAAGACGCCTATGCGTTATAACCCGGTGTTGGGAGTCGAGAAAGATCACCATAAAGATCTCCCGCTCCTCACCCGTGAGCTGGCTTTGTAAAAATTCCCTCGTCATCTCCGGGCTGAGTAAAGGGCTTTCTTCACGCATCCGCACGTTGTAGTAACGCCGCGCCAGtt  >  2:191201/1‑251 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                               CGATGTGCTCAAGTCCCGAACAGACAAAGATCCCGAAGGATCGCGCATAGCGGGTTAAATCCAGCCGCGTTCGGCAAAAGAAACATACTCTCCACGCCCAATCACGATATGGTCGAGCACGCGTAAATCCATGAACTGACAACTCTTTATTATCCGTTCAGTAATGAGTTTATCCGCTTTACTGGGTTCAGCACAACCCGAAGGGTGATTATGTGCAAGGATCAGCGCCGAGGCGTTTATTTTTATCGCTTCGCGGATAATTTCCCGAGGATGGACTTCAACATGGTTTAGCGTGCCGGAAAAAAGACGCCTATGCGTTATAACCCGGTGTTGGGAGTCGAGAAAGATCACCATAAAGATCTCCCGCTCCTCACCCGTGAGCTGGCTTTGTAAAAATTCCCTCGTCATCTCCGGGCTGAGTAAAGGGCTTTCTTCACGCATCCGCACGTTGTAGTAACGCCGCGCCAGTT  >  NC_000913/3811837‑3812306

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑