Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	711,853	G→A	H16Y (CAC→TAC)	ybfE ←	ribbon‑helix‑helix domain‑containing protein YbfE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	711,853	0	G	A	100.0%	216.8 / NA	66	H16Y (CAC→TAC)	ybfE	ribbon‑helix‑helix domain‑containing protein YbfE
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (32/34);  total (32/34)

AAACGATGCCCTGACTACGCAGCGCCGCCAGTTGCTGCATTAACATCTCTTCGATCAGTTCGCTACGGCTCATATTGCGCGACTCCGCCAGCTCGTTCAGCGCCTCGACAGCTTCCGCGTTCAGCTTCAGTTCGACACGCTTAAGGCCACGTACTTTGTCGCGTTTTAGCTGGTTGCGTTTATTAATACGCAGCTGTTCATCGCGCGAAAGCGGATTAGTTTTCGGTCGTCCCGGTCGACGCTCGTGCGCGAACAGATCTAATGTCGTACGGTCCGTTTGTTCTTTGGCCATGATCTTGGTGACTTCGGGGGAAACAATCAGCCAGGCCTCTGCCCGGATGGATAGCGCGCCATAATACATCAGCGCGATGAGTCACGCCAACGCCCACG‑CGCGGAAAGCGACGCGGACGCTGGGTTTTTAATCAGTTGCGTTAATCATTGAGATAGCGACGGATAGCGCGTAATACCGCATCCGGTTTTTCAGCATGGA  >  NC_000913/711607‑712096                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                     aaaCGATGCCCTGACTACGCAGCGCCGCCAGTTGCTGCATTAACATCTCTGCGATCAGTTCGCTACGGCTCATATTGCGCGACTCCGCCAGCTCGTTCAGCGCCTCGACAGCTTCCGCGTTCAGCTTCAGTTCGACACGCTTAAGGCCACGTACTTTGTCGCGTTTTAGCTGGTTGCGTTTATTAATACGCAGCTGTTCATCGCGCGAAAGCGGATTAGTTTTCGGTCGTCCCGGTCGACGCTCGTAcgcg                                                                                                                                                                                                                                                  <  2:298478/251‑1 (MQ=255)    cGATGCCCTGACTACGCAGCGCCGCCAGTTGCTGCATTAACATCTCTTCGATCAGTTCGCTACGGCTCATATTGCGCGACTCCGCCAGCTCGTTCAGCGCCTCGACAGCTTCCGCGTTCAGCTTCAGTTCGACACGCTTAAGGCCACGTACTTTGTCGCGTTTTAGCTGGTTGCGTTTATTAATACGCAGCTGTTCATCGCGCGAAAGCGGATTAGTTTTCGGTCGTCCCGGTCGACGCTCGTACGCGAAc                                                                                                                                                                                                                                               >  2:269315/1‑251 (MQ=255)         cccTGACTACGCAGCGCCGCCAGTTGCTGCATTAACATCTCTTCGATCAGTTCGCTACGGCTCATATTGCGCGACTCCGCCAGCTCGTTCAGCGCCTCGACAGCTTCCGCGTTCAGCTTCAGTTCGACACGCTTAAGGCCACGTACTTTGTCGCGTTTTAGCTGGTTGCGTTTATTAATACGCAGCTGTTCATCGCGCGAAAGCGGATTAGTTTTCGGTCGTCCCGGTCGACGCTCGTACGCGAACAGATc                                                                                                                                                                                                                                          <  1:418414/251‑1 (MQ=255)               cTACGCAGCGCCGCCAGTTGCTGCATTAACATCTCTTCGATCAGTTCGCTACGGCTCATATTGCGCGACTCCGCCAGCTCGTTCAGCGCCTCGACAGCTTCCGCGTTCAGCTTCAGTTCGACACGCTTAAGGCCACGTACTTTGTCGCGTTTTAGCTGGTTGCGTTTATTAATACGCAGCTGTTCATCGCGCGAAAGCGGATTAGTTTTCGGTCGTCCCGGTCGACGCTCGTACGCGAACAGATCTAATGt                                                                                                                                                                                                                                    >  2:269102/1‑251 (MQ=255)                 aCGCAGCGCCGCCAGTTGCTGCATTAACATCTCTTCGATCAGTTCGCTACGGCTCATATTGCGCGACTCCGCCAGCTCGTTCAGCGCCTCGACAGCTTCCGCGTTCAGCTTCAGTTCGACACGCTTAAGGCCACGTACTTTGTCGCGTTTTAGCTGGTTGCGTTTATTAATACGCAGCTGTTCATCGCGCGAAAGCGGATTAGTTTTCGGTCGTCCCGGTCGACGCTCGTACGCGAACAGATCTAATGTc                                                                                                                                                                                                                                   <  1:336096/250‑1 (MQ=255)                   gCAGCGCCGCCAGTTGCTGCATTAACATCTCTTCGATCAGTTCGCTACGGCTCATATTGCGCGACTCCGCCAGCTCGTTCAGCGCCTCGACAGCTTCCGCGTTCAGCTTCAGTTCGACACGCTTAAGGCCACGTACTTTGTCGCGTTTTAGCTGGTTGCGTTTATTAATACGCAGCTGTTCATCGCGCGAAAGCGGATTAGTTTTCGGTCGTCCCGGTCGACGCTCGTACGCGAACAGATCTAATGTCGt                                                                                                                                                                                                                                 >  1:344607/1‑250 (MQ=255)                        gccgccAGTTGCTGCATTAACATCTCTTCGATCAGTTCGCTACGGCTCATATTGCGCGACTCCGCCAGCTCGTTCAGCGCCTCGACAGCTTCCGCGTTCAGCTTCAGTTCGACACGCTTAAGGCCACGTACTTTGTCGCGTTTTAGCTGGTTGCGTTTATTAATACGCAGCTGTTCATCGCGCGAAAGCGGATTAGTTTTCGGTCGTCCCGGTCGACGCTCGTACGCGAACAGATCTAATGTCGTACGGTc                                                                                                                                                                                                                           >  2:374882/1‑251 (MQ=255)                          cgccAGTTGCTGCATTAACATCTCTTCGATCAGTTCGCTACGGCTCATATTGCGCGACTCCGCCAGCTCGTTCAGCGCCTCGACAGCTTCCGCGTTCAGCTTCAGTTCGACACGCTTAAGGCCACGTACTTTGTCGCGTTTTAGCTGGTTGCGTTTATTAATACGCAGCTGTTCATCGCGCGAAAGCGGATTAGTTTTCGGTCGTCCCGGTCGACGCTCGTACGCGAACAGATCTAATGTCGTACGGTCCg                                                                                                                                                                                                                         >  1:387560/1‑251 (MQ=255)                          cgccAGTTGCTGCATTAACATCTCTTCGATCAGTTCGCTACGGCTCATATTGCGCGACTCCGCCAGCTCGTTCAGCGCCTCGACAGCTTCCGCGTTCAGCTTCAGTTCGACACGCTTAAGGCCACGTACTTTGTCGCGTTTTAGCTGGTTGCGTTTATTAATACGCAGCTGTTCATCGCGCGAAAGCGGATTAGTTTTCGGTCGTCCCGGTCGACGCTCGTACGCGAACAGATCTAATGTCGTACGGTCCg                                                                                                                                                                                                                         >  1:25837/1‑251 (MQ=255)                           gccAGTTGCTGCATTAACATCTCTTCGATCAGTTCGCTACGGCTCATATTGCGCGACTCCGCCAGCTCGTTCAGCGCCTCGACAGCTTCCGCGTTCAGCTTCAGTTCGACACGCTTAAGGCCACGTACTTTGTCGCGTTTTAGCTGGTTGCGTTTATTAATACGCAGCTGTTCATCGCGCGAAAGCGGATTAGTTTTCGGTCGTCCCGGTCGACGCTCGTACGCGAACAGATCTAATGTCGTACGGTCCGt                                                                                                                                                                                                                        >  1:389615/1‑251 (MQ=255)                                    tgcATTAACATCTCTTCGATCAGTTCGCTACGGCTCATATTGCGCGACTCCGCCAGCTCGTTCAGCGCCTCGACAGCTTCCGCGTTCAGCTTCAGTTCGACACGCTTAAGGCCACGTACTTTGTCGCGTTTTAGCTGGTTGCGTTTATTAATACGCAGCTGTTCATCGCGCGAAAGCGGATTAGTTTTCGGTCGTCCCGGTCGACGCTCGGACGCGAACAGATCTAATGTCGTACGGTCCGTTTGTTCttt                                                                                                                                                                                                               >  2:245622/1‑251 (MQ=255)                                               ctctTCGATCAGTTCGCTACGGCTCATATTGCGCGACTCCGCCAGCTCGTTCAGCGCCTCGACAGCTTCCGCGTTCAGCTTCAGTTCGACACGCTTAAGGCCACGTACTTTGTCGCGTTTTAGCTGGTTGCGTTTATTAATACGCAGCTGTTCATCGCGCGAAGGCG‑ATTAGTTTTCGGTCGGCCCGGTCGACGCTCGTACGCGAACAGATCTAATGTCGTACGGTCCGTTTGTTCTTTGGCCATGATCtt                                                                                                                                                                                                   >  2:18871/1‑251 (MQ=255)                                                    cGATCAGTTCGCTACGGCTCATATTGCGCGACTCCGCCAGCTCGTTCAGCGCCTCGACAGCTTCCGCGTTCAGCTTCAGTTCGACACGCTTAAGGCCACGTACTTTGTCGCGTTTTAGCTGGTTGCGTTTATTAATACGCAGCTGTTCATCGCGCGAAAGCGGATTAGTTTTCGGTCGTCCCGGTCGACGCTCGTACGCGAACAGATCTAATGTCGTACGGTCCGTTTGTTCTTTGGCCATGATCTTGGt                                                                                                                                                                                                <  1:388138/250‑1 (MQ=255)                                                     gATCAGTTCGCTACGGCTCATATTGCGCGACTCCGCCAGCTCGTTCAGCGCCTCGACAGCTTCCGCGTTCAGCTTCAGTTCGACACGCTTAAGGCCACGTACTTTGTCGCGTTTTAGCTGGTTGCGTTTATTAATGCGCAGCTGTTCATCGCGCGAAAGCGGATTAGTTTTCGGTCGTCCCGGTCGACGCTCGTACGCGAACAGATCTAATGTCGTACGGTCCGTTTGTTCTTTGGCCATGATCTTGGTGa                                                                                                                                                                                              <  1:144864/251‑1 (MQ=255)                                                         aGTTCGCTACGGCTCATATTGCGCGACTCCGCCAGCTCGTTCAGCGCCTCGACAGCTTCCGCGTTCAGCTTCAGTTCGACACGCTTAAGGCCACGTACTTTGTCGCGTTTTAGCTGGTTGCGTTTATTAATACGCAGCTGTTCATCGCGCGAAAGCGGATTAGTTTTCGGTCGTCCCGGTCGACGCTCGTACGCGAACAGATCTAATGTCGTACGGTCCGTTTGTTCTTTGGCCATGATCTTGGTGACTTc                                                                                                                                                                                          >  2:123691/1‑251 (MQ=255)                                                              gCTACGGCTCATATTGCGCGACTCCGCCAGCTCGTTCAGCGCCTCGACAGCTTCCGCGTTCAGCTTCCGTTCGACACGCTTAAGGCCACGTACTTTGTCGCGTTTTAGCTGGTTGCGTTTATTAATACGCAGCTGTTCATCGCGCGAAAGCGGATTAGTTTTCGGTCGTCCCGGTCGACGCTCGTACGCGAACAGATCTAATGTCGTACGGCCCGTTTGTTCTTTGGCCATGATCTTGGTGACTTCggggg                                                                                                                                                                                     >  2:218871/1‑251 (MQ=255)                                                              gCTACGGCTCATATTGCGCGACTCCACCAGCTCGTTCAGCGCCTCGACAGCTTCCGCGTTCAGCTTCAGTTCGACACGCTTAAGGCCACGTACTTTGTCGCGTTTTAGCTGGTTGCGTTTATTAATACGCAGCTGTTCATCGCGCGAAAGCGGATTAGTTTTCGGTCGTCCCGGTCGACGCTCGTACGCGAACAGATCTAATGTCGTACGGTCCGTTTGTTCTTTGGCCATGATCTTGGTGACTTCggggg                                                                                                                                                                                     >  2:73145/1‑251 (MQ=255)                                                                   ggCTCATATTGCGCGACTCCGCCAGCTCGTTCAGCGCCTCGACAGCTTCCGCGTTCAGCTTCAGTTCGACACGCTTAAGGCCACGTACTTTGTCGCGTTTTAGCTGGTTGCGTTTATTAATACGCAGCTGTTCATCGCGCGAAAGCGGATTAGTTTTCGGTCGTCCCGGTCGACGCTCGTACGCGAACAGATCTAATGTCGTACGGTCCGTTTGTTCTTTGGCCATGATCTTGGTGACTTCGGGGGAAACa                                                                                                                                                                                <  2:146004/251‑1 (MQ=255)                                                                     cgcATATTGCTCGACTCCGCCAGCTCGTTCAGCGCCTCGACATCTTCCGCGTTCAGCTTCAGTTCGACACTCTTAAGTCCACGTACTTTGTCGCGTTTTAGCTGGTTGCGTTTATTAATACGCAGCTGTTCATCGCGCGAAAGCGGATTAGTTTTCGGTCGTCCCGGTCGACGCTCGTACGCGAACAGATCTAATGTCGTACGGTCCGTTTGTTCTTTGGCCATGATCTTGGTGACTTCGGGGGAAACAAt                                                                                                                                                                              <  2:83475/249‑1 (MQ=255)                                                                              cgcgACTCCGCCAGCTCGTTCAGCGCCTCGACAGCTTCCGCGTTCAGCTTCAGTTCGACACGCTTAAGGCCACGTACTTTGTCGCGTTTTAGCTGGTTGCGTTTATTAATACGCAGCTGTTCATCGCGCGAAAGCGGATTAGTTTTCGGTCGTCCCGGTCGACGCTCGTACGCGAACAGATCTAATGTCGTACGGTCCGTTTGTTCTTTGGCCATGATCTTGGTGACTTCGGGGGAAACAATCAGCCAGGc                                                                                                                                                                     >  2:240352/1‑251 (MQ=255)                                                                                         cAGCTCGTTCAGCGACTCGACAGCTCCCGCGTTCAGCTTCCGTTCGCCACGCTTAAGGCCACGTACCTTGTCGCGTTTTAGCTGGTTGCGTTTATTAATACGCAGATGTTCATCGCGCGAAAGCGGATTAGTTTTCGGTCGTACCGGTCGACGCTCGTACGCGAACAGATCTAATGTCGTCCGGTCCGTTTGTTCTTTGGCTATGATCTTGGTGACTTCGGGGGAAACAATCAGCCAGCCCTCTGCACgg                                                                                                                                                           >  2:308706/1‑250 (MQ=255)                                                                                                 tCAGCGCCTCGACAGCTTCCGCGTTCAGCTTCAGTTCGACACGCTTAAGGCCACGTACTTTGTCGCGTTTTAGCTGGTTGCGTTTATTAATACGCAGCTGTTCATCGCGCGAAAGCGGATTAGTTTTCGGTCGTCCCGGTCGACGCTCGTACGCGAACAGATCTAATGTCGTACGGTCCGTTTGTTCTTTGGCCATGATCTTGGTGACTTCGGGGGAAACAATCAGCCAGGCCTCTGCCCGGATGGATAg                                                                                                                                                   <  2:16543/250‑1 (MQ=255)                                                                                                      gccccGACAGCTTCCGCGTTCAGCTTCAGTTCGACACGCTTAAGGCCACGTACTGTGTCGCGTTTTAGCTGGTTGCGTTTATTAATACGCAGCGGTTCATCGCGCGAAAGCGGATTAGTTTTCGGTCGTCCCGGTCGACGCTCGTACGCGAACAGATCTAATGTCGTACGGTCCGTTTGTTCGTTGGCCATGATCTTGGTGACTTCGGGGTAAACAATCAGCCAGGCCTCTGCCCGGATGGATAGCGCGcc                                                                                                                                             <  2:72335/247‑1 (MQ=255)                                                                                                                   ccGCGTTCAGCTTCAGTTCGACACGCTTAAGGCCACGTACTTTGTCGCGTTTTAGCTGGTTGCGTTTATTAATACGCAGCTGTTCATCGCGCGAAAGCGGATTAGTTTTCGGTCGTCCCGGTCGACGCTCGTACGCGAACAGATCTAATGTCGTACGGTCCGTTTGTTCTTTGGCCATGATCTTGGTGACTTCGGGGGAAACAATCAGCCAGGCCTCTGCCCGGATGGATAGCGCGCCATAATACATCAg                                                                                                                                 <  2:423297/250‑1 (MQ=255)                                                                                                                    cgcgTTCAGCTTCAGTTCGACACGCTTAAGGCCACGTACTTTGTCGCGTTTTAGCTGGTTGCGTTTATTAATACGCAGCTGTTCATCGCGCGAAAGCGGATTAGTTTTCGGTCGTCCCGGTCGACGCTCGTACGCGAACAGATCTAATGTCGTACGGTCCGTTTGTTCTTTGGCCATGATCTTGGTGACTTCGGGGGAAACAATCAGCCAGGCCTCTGCCCGGATGGATAGCGCGCCATAATACATCAgcg                                                                                                                               >  1:209752/1‑251 (MQ=255)                                                                                                                         tCAGCTTCAGTTCGACACGCTTAAGGCCACGTACTTTGTCGCGTTTTAGCTGGTTGCGTTTATTAATACGCAGCTGTTCATCGCGCGAAAGCGGATTAGTTTTCGGTCGTCCCGGTCGACGCTCGTACGCGAACAGATCTAATGTCGTACGGTCCGTTTGTTCTTTGGCCATGATCTTGGTGACTTCGGGGGAAACAATCAGCCAGGCCTCTGCCCGGATGGATAGCGCGCCATAATACATCAGCGCGAt                                                                                                                           <  2:331906/250‑1 (MQ=255)                                                                                                                              ttCAGTTCGACACGCTTAAGGCCACGTACTTTGTCGCGTTTTAGCTGGTTGCGTTTATTAATACGCAGCTGTTCATCGCGCGAAAGCGGATTAGTTTTCGGTCGTCCCGGTCGACGCTCGTACGCGAACAGATCTAATGTCGTACGGTCCGTTTGTTCTTTGGCCATGATCTTGGTGACTTCGGGGGAAACAATCAGCCAGGCCTCTGCCCGGATGGATAGCGCGCCATAATACATCAGCGCGATGAGTc                                                                                                                      >  1:380918/1‑250 (MQ=255)                                                                                                                                cAGTTCGACACGCTTAAGGCCACGTACTTTGTCGCGTTTTAGCTGGTTGCGTTTATTAATACGCAGCTGTTCATCGCGCGAAAGCGGATTAGTTTTCGGTCGTCCCGGTCGACGCTCGTACGCGAACAGATCTAATGTCGTACGGTCCGTTTGTTCTTTGGCCATGATCTTGGTGACTTCGGGGGAAACAATCAGCCAGGCCTCTGCCCGGATGGATAGCGCGCCATAATACATCAGCGCGATGAGTCACg                                                                                                                   >  1:137092/1‑251 (MQ=255)                                                                                                                                    tCGACACGCTTAAGCCCACGTCCTTTGTCGCGTTTTAACTGGTTGCGTTTATTACTACGCAGCTGTTCATCGCGCGAAAGCGGATTAGTTTTCGGTCGTCCCGGTCGACGCTCGTACGCGACCAGATCTAATGTCGTACGGTCCGTTTGTTCTTTGGCCATGATCTTGGTGACTTCGGGGGAAACAATCAGCCAGGCCTCTGCCCGGATGGATAGCGCGCCATAATACATCAGCGCGATGAGTCACGNCa                                                                                                                >  2:410903/1‑250 (MQ=255)                                                                                                                                      gACACGCTTAAGGCCACGTACTTTGTCGCGTTTTAGCTGGTTGCGTTTATTAATACGCAGCTGTTCATCGCGCGAAAGCGGATTAGTTTTCGGTCGTCCCGGTCGACGCTCGTACGCGAACAGATCTAATGTCGTACGGTCCGTTTGTTCTTTGGCCATGATCTTGGTGACTTCGGGGGAAACAATCAGCCAGGCCTCTGCCCGGATGGATAGCGCGCCATAATACATCAGCGCGATGAGTCACGCCAACg                                                                                                             >  2:328631/1‑251 (MQ=255)                                                                                                                                      gACACGCTTAAGGCCACGTACTTTGTCGCGTTTTAGCTGGTTGCGTTTATTAATACGCAGCTGTTCATCGCGCGAAAGCGGATTAGTTTTCGGTCGTCCCGGTCGACGCTCGTACGCGAACAGATCTAATGTCGTACGGTCCGTTTGTTCTTTGGCCATGATCTTGGTGACTTCGGGGGAAACAATCAGCCAGGCCTCTGCCCGGATGGATAGCGCGCCATAATACATCAGCGCGATGAGCCACGCCAACg                                                                                                             >  2:404120/1‑251 (MQ=255)                                                                                                                                        cacGCTTAAGGCCACGTACTTTGTCGCGTTTTAGCTGGTTGCGTTTATTAATACGCAGCTGTTCATCGCGCGAAAGCGGATTAGTTTTCGGTCGTCCCGGTCGACGCTCGTACGCGAACAGATCTAATGTCGTACGGTCCGTTTGTTCTTTGGCCATGATCTTGGTGACTTCGGGGGAAACAATCAGCCAGGCCTCTGCCCGGATGGATAGCGCGCCATAATACATCAGCGCGATGAGTCACGCCAACGcc                                                                                                           <  1:328631/251‑1 (MQ=255)                                                                                                                                        cacGCTTAAGGCCACGTACTTTGTCGCGTTTTAGCTGGTTGCGTTTATTAATACGCAGCTGTTCATCGCGCGAAAGCGGATTAGTTTTCGGTCGTCCCGGTCGACGCTCGTACGCGAACAGATCTAATGTCGTACGGTCCGTTTGTTCTTTGGCCATGATCTTGGTGACTTCGGGGGAAACAATCAGACAGGCCTCTGCCCGGATGAATAGCGCGTCATAATACATCAGCGCGCTGAGTCACGCCacagcc                                                                                                           >  1:189500/1‑246 (MQ=255)                                                                                                                                                     aCGTACTTTGTCGCGTTTTAGCTGGTTGCGTTTATTAATACGCAGCTGTTCATCGCGCGAAAGCGGATTAGTTTTCGGTCGTCCCGGTCGACGCTCGTACGCGAACAGATCTAATGTCGTACGGTCCGTTTGTTCTTTGGCCATGATCTTGGTGACTTCGGGGGAAACAATCAGCCAGGCCTCTGCCCGGATGGATAGCGCGCCATAATACATCAGCGCGATGAGTCACGCCAACGCCCACG‑CGCGGAAAg                                                                                             <  1:67667/251‑1 (MQ=255)                                                                                                                                                      cGTACTTTGTCGCGTTTTAGCTGGTTGCGTTTATTAATACGCAGCTGTTCATCGCGCGAAAGCGGATTAGTTTTCGGTCGTCCCGGTCGACGCTCGTACGCGAACAGATCTAATGTCGTACGGTCCGTTTGTTCTTTGGCCATGATCTTGGTGACTTCGGGGGAAACAATCAGCCAGGCCTCTGCCCGGATGGATAGCGCGCCATAATACATCAGCGCGATGAGTCACGCCAACGCCCACG‑CGCGGAAAGc                                                                                            <  1:135113/251‑1 (MQ=255)                                                                                                                                                         aCTTTGTCGCGTTTTAGCTGGTTTCGTTTATTAATACGCAGCTGTTCATCGCGCGAAAGCG‑ATTAGTTTTCGGTCGTCCCGGTCGACGCTCGTACGCGAACAGATCTAATGTCGTACGGTCCGTTTGTTCTTTGGCCATGATCTTGGTGACTTCGGGGGAAACAATCAGCCAGGCCTCTGCCCGGATGGATAGCGCGCCATAATACATCAGCGCGATGAGTCACGCCAACGCCCACG‑CGCGGAAAGCGAcg                                                                                        <  1:18871/251‑1 (MQ=255)                                                                                                                                                          cTTTGTCGCGTTTTAGCTGGTTGCGTTTATTAATACGCAGCTGTTCATCGCGCGAAAGCGGATTAGTTTTCGGTCGTCCCGGTCGACGCTCGTACGCGAACAGATCTAATGTCGTACGGTCCGTTTGTTCTTTGGCCATGATCTTGGTGACTTCGGGGGAAACAATCAGCCAGGCCTCTGCCCGGATGGATAGCGCGCCATAATACATCAGCGCGATGAGTCACGCCAACACACACG‑CGCGGAAAGAGAcg                                                                                        >  1:42980/1‑251 (MQ=255)                                                                                                                                                                cgcgTTTTAGCTGGTTGCGTTTATTAATACGCAGCTGTTCATCGCGCGAAAGCGGATTAGTTTTCGGTCGTCCCGGTCGACGCTCGTACGCGAACAGATCTAATGTCGTACGGTCCGTTTGTGCTTTGGCCATGATCTTGGTGACTTCGGGGGAAACAATCAGCCAGGCCTCTGCCCGGATGGATAGCGCGCCAGAATACATCAGCGCGATGAGTCACGCCAACGCCCACG‑CGCGGAAAGCGACGCGGACg                                                                                  <  2:25837/251‑1 (MQ=255)                                                                                                                                                                 gcgTTTTCGGTGGTTGCGTTTATTAATACGCAGCTGTTCATCGCGCGAACGCGGATTAGATTTCGGTAGTCCCGGTCGACGCTCGTACGCGAACAGATCTAATGTCGTACGGCCCGTTTGTTCTTTGGCCATGATCTTGATGACTTCGGGGGAAACAATCAGCCAGGCCTCTGCCCGGATGGATAGCGCGCCATAATACATCAGCGCGCTGCGTCACGCACACGACCACG‑CGCGGAAAGAGACGCGGACGc                                                                                 >  2:255620/1‑251 (MQ=255)                                                                                                                                                                 gcgTTTTAGCTGGTTGCGTTTATTAATACGCAGCTGTTCATCGCGCGAAAGCGGATTAGTTTTCGGTCGTCCCGGTCGACGCTCGTACGCGAACAGATCTAATGTCGTACGGTCCGTTTGTTCTTTGGCCATGATCTTGGTGACTTCGGGGGAAACAATCAGCCAGGCCTCTGCCCGGATGGATAGCGCGCCATAATACATCAGCGCGATGAGTCACGCCAACGCCCACG‑CGCGGAAAGCGACGCGGACGc                                                                                 <  1:123691/251‑1 (MQ=255)                                                                                                                                                                   gTTTTAGCTGGTTGCGTTTATTAATACGCAGCTGTTCATCGCGCGAAAGCGGATTAGTTTTCGGTCGTCCCGGTCGACGCTCGTACGCGAACAGATCTAATGTCGTACGGTCCGTTTGTTCTTTGGCCATGATCTTGGTGACTTCGGGGGAAACAATCAGCCAGGCCTCTGCCCGGATGGATAGCGCGCCATAATACATCAGCGCGATGAGTCACGCCAACGCCCACG‑CGCGGAAAGCGACGCGGACGCTg                                                                               >  2:393785/1‑251 (MQ=255)                                                                                                                                                                     tttAGCTGGTTGCGTTTATTAATACGCAGCTGTTCATCGCGCGAAAGCGGATTAGTTTTCGGTCGTCCCGGTCGACGCTCGTACGCGAACAGATCTAATGTCGTACGGTCCGTTTGTTCTTTGGCCATGATCTTGGTGACTTCGGGGGAAACAATCAGCCAGGCCTCTGCCCGGATGGATAGCGCGCCATAATACATCAGCGCGATGAGTCACGCCAACGCCCACG‑CGCGGAAAGCGACGCGGACGCTggg                                                                             >  1:192756/1‑251 (MQ=255)                                                                                                                                                                       tAGCTGGTTGCGTTTATTAATACGCAGCTGTTCATCGCGCGAAAGCGGATTAGTTTTCGGTCGTCCCGGTCGACGCTCGTACGCGAACAGATCTAATGTCGTACGGTCCGTTTGTTCTTTGGCCATGATCTTGGTGACTTCGGGGGAAACAATCAGCCAGGCCTCTGCCCGGATGGATAGCGCGCCATAATACATCAGCGCGATGAGTCACGCCAACGCCCACG‑CGCGGAAAGCGACGCGGACGCTGGGt                                                                            <  1:253702/250‑1 (MQ=255)                                                                                                                                                                        aGCTGGTTGCGTTTATTAATACGCAGCTGTTCATCGCGCGAAAGCGGATTAGTTTTCGGTCGTCCCGGTCGACGCTCGTACGCGAACAGATCTAATGTCGTACGGTCCGTTTGTTCTTTGGCCATGATCTTGGTGACTTCGGGGGAAACAATCAGCCAGGCCTCTGCCCGGATGGATAGCGCGCCATAATACATCAGCGCGATGAGTCACGCCAACGCCCACG‑CGCGGAAAGCGACGCGGACGCTGGGtt                                                                           <  1:126924/250‑1 (MQ=255)                                                                                                                                                                          cttgTTGCGTTTATTAATACGCAGCTGTTCATCGCGCGAAAGCGGATTAGTTTTCGGTCGTCCCGGTCGACGCTCGTACGCGAACAGATCTAATGTCGTACGGTCCGTTTGTTCTTTGGCCATGATCTTGGTGACTTCGGGGGAAACAATCAGCCAGGCCTCTGCCCGGATGGATAGCGCGCCATAAGACATCAGCGCGATGAGTCTCGCCAACGCCCACG‑CGCGGAAAGCGACGCGGACGCTGGGtt                                                                           <  1:31457/245‑1 (MQ=255)                                                                                                                                                                          cTGGTTGCGTTTATTAATACGCAGCTGTTCATCGCGCGAAAGCGGATTAGTTTTCGGTCGTCCCGGTCGACGCTCGTACGCGACCAGATCTAATGTCGTACGGTCCGTTTGTTCTTTGGCCATGATCTTGGTGACTTCGGGGGAAACACTCAGCCAGGCCTCTGCCCGGATGGATAGCGCGCCATAATACATAAGCGCGATGAGTCACGCCAACGCCCACG‑CGCGGAAAGCGACGCGGACGCTGGGtt                                                                           >  2:31457/1‑248 (MQ=255)                                                                                                                                                                            ggTTGCGTTTATTAATACGCAGCTGTTCATCGCGCGAAAGCGGATTAGTTTTCGGTCGTCCCGGTCGACGCTCGTACGCGAACAGATCTAATGTCGTACGGTCCGTTTGTTCTTTGGCCATGATCTTGGTGACTTCGGGGGAAACAATCAGCCAGGCCTCTGCCCGGATGGATAGCGCGCCATAATACATCAGCGCGATGAGTCACGCCAACGCCCACG‑CGCGGAAAGCGACGCGGACGCTGGGTTTTTa                                                                       >  1:429799/1‑250 (MQ=255)                                                                                                                                                                             gTTGCGTTTATTAATACGCAGCTGTTCATCGCGCGAAAGCGGATTAGTTTTCGGTCGTCCCGGTCGACGCTCGTACGCGAACAGATCTAATGTCGTACGGTCCGTTTGTTCTTTGGCCATGATCTTGGTGACTTCGGGGGAAACAATCAGCCAGGCCTCTGCCCGGATGGATAGCGCGCCATAATACATCAGCGCGATGAGTCACGCCAACGCCCACG‑CGCGGAAAGCGACGCGGACGCTGGGTTTTTAAt                                                                     <  1:374882/251‑1 (MQ=255)                                                                                                                                                                             gTTGCGTTTATTAATACGCAGCTGTTCATCGCGCGAAAGCGGATTAGTTTTCGGTCGTCCCGGTCGACGCTCGTACGCGAACAGATCTAATGTCGTACGGTCCGTTTGTTCTTTGGCCATGATCTTGGTGACTTCGGGGGAAACAATCAGCCAGGCCTCTGCCCGGATGGATAGCGCGCCATAATACATCAGCGCGATGAGTCACGCCAACGCCCACG‑CGCGGAAAGCGACGCGGACGCTGGGATTTTAAt                                                                     <  2:209752/251‑1 (MQ=255)                                                                                                                                                                             gTTGCGTTTATTAATACGCAGCTGTTCATCGCGCGAAAGCGGATTAGTTTTCGGTCGTCCCGGTCGACGCTCGTACGCGAACAGATCTAATGTCGTACGGTCCGTTTGTTCTTTGGCCATGATCTTGGTGACTTCGGGGGAAACAATAAGCCAGGCCTTTGCCCGGATGGATAGCGCGCCATAAAACATAGGCGTGATGGGTAAGGCCAAC‑CCCACGGCGCGGAAGGGGACCAGGACGCGGGGTTTTTatc                                                                     >  1:208348/1‑249 (MQ=255)                                                                                                                                                                               tGCGTTTATTAATACGCAGCTGTTCATCGCGCGAAAGCGGATTAGTTTTCGGTCGTCCCGGTCGACGCTCGTACGCGAACAGATCTAATGTCGTACGGTCCGTTTGTTCTTTGGCCATGATCTTGGTGACTTCGGGGGAAACAATCAGCCAGGCCTCTGCCCGGATGGATAGCGCGCCATAATACATCAGCGCGATGAGTCACGCCAACGCCCACG‑CGCGGAAAGCGACGCGGACGCTGGGTTTTTAATc                                                                    <  1:393785/250‑1 (MQ=255)                                                                                                                                                                                gCGTTTATTAATTCGCAGCTGTTCATCGCGCGAAAGCGGATTAGTTTTCGGTCGTCCCGGTCGACGCTCGTACGCGAACAGATCTAATGTCGTACGGTCCGTTTGTTCTTTGGCCATGATCTTGGTGACTTCGGGGGAAACAATCAGCCAGGCCTCTGCCCGGATGGATAGCGCGCCATAATACATCAGCGCGATGAGTCACGCCAACGCCCACG‑CGCGGAAAGCGACGCGGACGCTGGGTTTTTAATCAg                                                                  <  2:321367/251‑1 (MQ=255)                                                                                                                                                                                  gTTTATTAATACGCAGCTGTTCATCGCGCGAAAGCGGATTAGTTTTCGGTCGTCCCGGTCGACGCTCGTACGCGAACAGATCTAATGTCGTACGGTCCGTTTGTTCTTTGGCCATGATCTTGGTGACTTCGGGGGAAACAATCAGCCAGGCCTCTGCCCGGATGGATAGCGCGCCATAATACATCAGCGCGATGAGTCACGCCAACGCCCACG‑CGCGGAAAGCGACGCGGACGCTGGGTTTTTAATCAGtt                                                                <  1:269315/251‑1 (MQ=255)                                                                                                                                                                                    ttattaATACGCAGCTGTTCATCGCGCGAAAGCGGATTAGTTTTCGGTCGTCCCGGTCGACGCTCGTACGCGAACAGATCTAATGTCGTACGGTCCGTTTGTTCTTTGGCCATGATCTTGGTGACTTCGGGGGAAACAATCAGCCAGGCCTCTGCCCGGATGGATAGCGCGCCATAATACATCAGCGCGATGAGTCACGCCAACGCCCACG‑CGCGGAAAGCGACGCGGACGCTGGGTTTTTAATCAGTTg                                                               <  1:272924/250‑1 (MQ=255)                                                                                                                                                                                      attaATACGCAGCTGTTCATCGCGCGAAAGCGGATTAGTTTTCGGTCGTCCCGGTCGACGCTCGTACGCGAACAGATCTAATGTCGTACGGTCCGTTTGTTCTTTGGCCATGATCTTGGTGACTTCGGGGGAAACAATCAGCCAGGCCTCTGCCCGGATGGATAGCGCGCCATAATACATCAGCGCGATGAGTCACGCCAACGCCCACG‑CGCGGAAAGCGACGCGGACGCTGGGTTTTTAATCAGTTGCg                                                             <  1:240352/250‑1 (MQ=255)                                                                                                                                                                                      attaATACGCAGCTGTTCATCGCGCGAAAGCGGATTAGTTTTCGGTCGTCCCGGTCGACGCTCGTACGCGAACAGATCTAATGTCGTACGGTCCGTTTGTTCTTTGGCCATGATCTTGGTGACTTCGGGGGAAACAATCAGCCAGGCCTCTGCCCGGATGGATAGCGCGCCATAATACATCAGCGCGATGAGTCACGCCAACGCCCACG‑CGCGGAAAGCGACGCGGACGCTGGGTTTTTAATCAGTTGCGt                                                            <  2:29758/251‑1 (MQ=255)                                                                                                                                                                                       ttaATACGCAGCTGTTCATCGCGCGAAAGCGGATTAGTTTTCGGTCGTCCCGGTCGACGCTCGTACGCGAACAGATCTAATGTCGTACGGTCCGTTTGTTCTTTGGCCATGATCTTGGTGACTTCGGGGGAAACAATCAGCCAGGCCTCTGCCCGGATGGATAGCGCGCCATAATACATCAGCGCGATGAGTCACGCCAACGCCCACG‑CGCGGAAAGCGACGCGGCCGCTGGGTTTTTAATCTGTTGCGtt                                                           >  2:214016/1‑251 (MQ=255)                                                                                                                                                                                                aGCTGTTCATCGCGCGAAAGCGGATTAGTTTTCGGTCGTCCCGGTCGACGCTCGTACGCGAACAGATCTAATGTCGTACGGTCCGTTTGTTCTTTGGCCATGATCTTGGTGACTTCGGGGGAAACAATCAGCCAGGCCTCTGCCCGGATGGATAGCGCGCCATAATACATCAGCGCGATGAGTCACGCCAACGCCCACG‑CGCGGAAAGCGACGCGGACGCTGGGTTTTTAATCAGTTGCGTTAATCATTga                                                  <  1:269102/251‑1 (MQ=255)                                                                                                                                                                                                           gcgcgAAAGCGGATTAGTTTTCGGTCGTCCCGGTCGACGCTCGTACGCGAACAGATCTAATGTCGTACGGTCCGTTTGTTCTTTGGCCATGATCTTGGTGACTTCGGGGGAAACAATCAGCCAGGCCTCTGCCCGGATGGATAGCGCGCCATAATACATCAGCGCGATGAGTCACGCCAACGCCCACG‑CGCGGAAAGCGACGCGGACGCTGGGTTTTTAATCAGTTGCGTTAATCATTGAGATAGCGACgg                                       <  2:169514/251‑1 (MQ=255)                                                                                                                                                                                                                    cGGATTAGTTTTCGGTCGTCCCGGTCGACGCTCGTACGCGAACAGATCTAATGTCGTACGGTCCGTTTGTTCTTTGGCCATTATCTTGGTGACTTCGGGGGAAACAAGCAGCCAGGCCTCTGCCCGGATGGATAGCGCGCCATAATACATCAGCGCGATGAGTCACGCCAACGCCCACG‑CGCGGAAAGCGAGTCGGACGCTGGGTTTTTAATCAGTTGCGTTAATCATTGAGATAGCGACGGATAGCGCGt                              <  1:178608/251‑1 (MQ=255)                                                                                                                                                                                                                          aGTTTTCGGTCGTCCCGGTCGACGCTCGTACGCGAACAGATCTAATGTCGTACGGTCCGTTTGTTCTTTGGCCATGATCTTGGTGACTTCGGGGGAAACAATCAGCCAGGCCTCTGCCCGGATGGATAGCGCGCCATAATACATCAGCGCGATGAGTCACGCCAACGCCCACG‑CGCGGAAAGCGACGCGGACGCTGGGTTTTTAATCAGTTGCGTTAATCATTGAGATAGCGACGGATAGCGCGTAATAcc                        <  1:73145/251‑1 (MQ=255)                                                                                                                                                                                                                                     gtcCCGGTCGACGCTCGTACGCGAACAGATCTAATGTCGTACGGTCCGTTTGTTCTTTGGCCATGATCTTGGTGACTTCGGGGGAAACAATCAGCCAGGCCTCTGCCCGGATGGATAGCGCGCCATAATACATCAGCGCGATGAGTCACGCCAACGCCCACG‑CGCGGAAAGCGACGCGGACGCTGGGTTTTTAATCAGTTGCGTTAATCATTGAGATAGCGACGGATAGCGCGTAATACCGCATCCGGttt             <  1:122246/251‑1 (MQ=255)                                                                                                                                                                                                                                         cGGTCGACGCTCGTACGCGAACAGATCTAATGTCGTACGGTCCGTTTGTTCTTTGGCCATGATCTTGGTGACTTCGGGGGAAACAATCAGCCAGGCCTCTGCCCGGATGGATAGCGCGCCATAATACATCAGCGCGATGAGTCACGCCAACGCCCACG‑CGCGGAAAGCGACGCGGACGCTGGGTTTTTAATCAGTTGCGTTAATCATTGAGATAGCGACGGATAGCGCGTAATACCGCATCCGGTTTTTc          >  2:37131/1‑250 (MQ=255)                                                                                                                                                                                                                                              gACGCTCGTACGCGAACAGATCTAATGTCGTACGGTCCGTTTGTTCTTTGGCCATGATCTTGGTGACTTCGGGGGAAACAATCAGCCAGGCCTCTGCCCGGATGGATAGCGCGCCATAATACATCAGCGCGATGAGTCACGCCAACGCCCACG‑CGCGGAAAGCGACGCGGACGCTGGGTTTTTAATCAGTTGCGTTAATCATTGAGATAGCGACGGATAGCGCGTAATACCGCATCCGGTTTTTCAGCATg    >  1:55873/1‑251 (MQ=255)                                                                                                                                                                                                                                               aCGCTCGTACGCGAACAGATCTAATGTCGTACGGTCCGTTTGTTCTTTGGCCATGATCTTGGTGACTTCGGGGGAAACAATCAGCCAGGCCTCTGCCCGGATGGATAGCGAGCCATAATACATCAGCGCGATGAGTCACGCCAACGCCCACG‑CGCGGAAAGCGACGCGGACCCTGGGTTTTTAATCAGTTGCGTTACTCATTGAGATAGCGACGGATAGCGCGTAATACCGCATCCGGTTTTTAAGCATgg   >  1:84069/1‑251 (MQ=255)                                                                                                                                                                                                                                                cGCTCGTACGCGAACAGATCTAATGTCGTACGGTCCGTTTGTTCTTTGGCCATGATCTTGGTGACTTCGGGGGAAACAATCAGCCAGGCCTCTGCCCGGATGGATAGCGCGCCATAATACATCAGCGCGATGAGTCACGCCAACGCCCACG‑CGCGGAAAGCGACGCGGACGCTGGGTTTTTAATCAGTTGCGTTAATCATTGAGATAGCGACGGATAGCGCGTAATACCGCATCCGGTTTTTCAGCATGGa  <  1:245622/251‑1 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                     AAACGATGCCCTGACTACGCAGCGCCGCCAGTTGCTGCATTAACATCTCTTCGATCAGTTCGCTACGGCTCATATTGCGCGACTCCGCCAGCTCGTTCAGCGCCTCGACAGCTTCCGCGTTCAGCTTCAGTTCGACACGCTTAAGGCCACGTACTTTGTCGCGTTTTAGCTGGTTGCGTTTATTAATACGCAGCTGTTCATCGCGCGAAAGCGGATTAGTTTTCGGTCGTCCCGGTCGACGCTCGTGCGCGAACAGATCTAATGTCGTACGGTCCGTTTGTTCTTTGGCCATGATCTTGGTGACTTCGGGGGAAACAATCAGCCAGGCCTCTGCCCGGATGGATAGCGCGCCATAATACATCAGCGCGATGAGTCACGCCAACGCCCACG‑CGCGGAAAGCGACGCGGACGCTGGGTTTTTAATCAGTTGCGTTAATCATTGAGATAGCGACGGATAGCGCGTAATACCGCATCCGGTTTTTCAGCATGGA  >  NC_000913/711607‑712096

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑