breseq  version 0.39.0  
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 19,111 C→T H133Y (CAC→TAC)  nhaR → DNA‑binding transcriptional activator NhaR
RA 64,536 C→T V415V (GTG→GTA polB ← DNA polymerase II
RA 64,539 T→C S414S (TCA→TCG polB ← DNA polymerase II
RA 64,593 C→T P396P (CCG→CCA polB ← DNA polymerase II
RA 64,635 A→G H382H (CAT→CAC polB ← DNA polymerase II
RA 64,641 T→A R380R (CGA→CGT polB ← DNA polymerase II
RA 64,644 C→A P379P (CCG→CCT polB ← DNA polymerase II
RA 64,665 T→C A372A (GCA→GCG polB ← DNA polymerase II
RA 64,677 A→T G368G (GGT→GGA polB ← DNA polymerase II
RA 64,680 G→C G367G (GGC→GGG polB ← DNA polymerase II
RA 64,686 T→G R365R (CGA→CGC polB ← DNA polymerase II
RA 64,692 C→T V363V (GTG→GTA polB ← DNA polymerase II
RA 64,713 T→C A356A (GCA→GCG polB ← DNA polymerase II
RA 64,716 C→T R355R (CGG→CGA polB ← DNA polymerase II
RA 64,749 G→A H344H (CAC→CAT polB ← DNA polymerase II
RA 64,755 G→A I342I (ATC→ATT polB ← DNA polymerase II
RA 64,794 T→C A329A (GCA→GCG polB ← DNA polymerase II
RA 64,883 G→A L300L (CTA→TTA)  polB ← DNA polymerase II
RA 64,896 A→G T295T (ACT→ACC polB ← DNA polymerase II
RA 65,028 G→A S251S (AGC→AGT polB ← DNA polymerase II
RA 65,031 A→G N250N (AAT→AAC polB ← DNA polymerase II
RA 65,043 A→C L246L (CTT→CTG polB ← DNA polymerase II
RA 65,046 A→C R245R (CGT→CGG polB ← DNA polymerase II
RA 65,057 G→T L242I (CTT→ATT)  polB ← DNA polymerase II
RA 65,169 C→T L204L (TTG→CTA polB ← DNA polymerase II
RA 65,171 A→G L204L (TTG→CTA)  polB ← DNA polymerase II
RA 65,202 G→A F193F (TTC→TTT polB ← DNA polymerase II
RA 65,208 A→G L191L (CTT→CTC polB ← DNA polymerase II
RA 65,213 A→C S190A (TCG→GCG)  polB ← DNA polymerase II
RA 65,232 C→T P183P (CCG→CCA polB ← DNA polymerase II
RA 65,316 T→A I155I (ATA→ATT polB ← DNA polymerase II
RA 65,381 T→C T134A (ACT→GCT)  polB ← DNA polymerase II
RA 65,454 A→G R109R (CGT→CGC polB ← DNA polymerase II
RA 65,594 A→C F63V (TTT→GTT)  polB ← DNA polymerase II
RA 65,785 A→T intergenic (‑5/+70) polB ← / ← araD DNA polymerase II/L‑ribulose‑5‑phosphate 4‑epimerase AraD
RA 65,788 A→T intergenic (‑8/+67) polB ← / ← araD DNA polymerase II/L‑ribulose‑5‑phosphate 4‑epimerase AraD
RA 65,811 G→T intergenic (‑31/+44) polB ← / ← araD DNA polymerase II/L‑ribulose‑5‑phosphate 4‑epimerase AraD
RA 65,905 C→T D216N (GAT→AAT)  araD ← L‑ribulose‑5‑phosphate 4‑epimerase AraD
RA 66,024 Δ1 bp coding (527/696 nt) araD ← L‑ribulose‑5‑phosphate 4‑epimerase AraD
RA 66,032 C→T P173P (CCG→CCA araD ← L‑ribulose‑5‑phosphate 4‑epimerase AraD
RA 66,041 G→A S170S (TCC→TCT araD ← L‑ribulose‑5‑phosphate 4‑epimerase AraD
RA 66,053 A→G V166V (GTT→GTC araD ← L‑ribulose‑5‑phosphate 4‑epimerase AraD
RA 66,092 A→G F153F (TTT→TTC araD ← L‑ribulose‑5‑phosphate 4‑epimerase AraD
RA 66,134 G→A G139G (GGC→GGT araD ← L‑ribulose‑5‑phosphate 4‑epimerase AraD
RA 66,227 A→G G108G (GGT→GGC araD ← L‑ribulose‑5‑phosphate 4‑epimerase AraD
RA 66,245 G→A T102T (ACC→ACT araD ← L‑ribulose‑5‑phosphate 4‑epimerase AraD
RA 66,263 C→T T96T (ACG→ACA araD ← L‑ribulose‑5‑phosphate 4‑epimerase AraD
RA 66,266 A→G H95H (CAT→CAC araD ← L‑ribulose‑5‑phosphate 4‑epimerase AraD
RA 66,287 G→C P88P (CCC→CCG araD ← L‑ribulose‑5‑phosphate 4‑epimerase AraD
RA 66,308 C→T R81R (CGG→CGA araD ← L‑ribulose‑5‑phosphate 4‑epimerase AraD
RA 66,323 G→A D76D (GAC→GAT araD ← L‑ribulose‑5‑phosphate 4‑epimerase AraD
RA 66,343 T→C T70A (ACG→GCG)  araD ← L‑ribulose‑5‑phosphate 4‑epimerase AraD
RA 66,403 C→T V50I (GTC→ATC)  araD ← L‑ribulose‑5‑phosphate 4‑epimerase AraD
RA 66,494 G→A H19H (CAC→CAT araD ← L‑ribulose‑5‑phosphate 4‑epimerase AraD
RA 66,518 T→C E11E (GAA→GAG araD ← L‑ribulose‑5‑phosphate 4‑epimerase AraD
MC JC 66,633 Δ9,290 bp araA[sgrR] araA, araB, araC, yabI, thiQ, thiP, thiB, sroA, [sgrR]
RA 75,938 T→A L454L (CTA→CTT sgrR ← DNA‑binding transcriptional dual regulator SgrR
RA 75,950 A→G S450S (AGT→AGC sgrR ← DNA‑binding transcriptional dual regulator SgrR
RA 75,953 T→C E449E (GAA→GAG sgrR ← DNA‑binding transcriptional dual regulator SgrR
RA 75,966 2 bp→TC coding (1333‑1334/1656 nt) sgrR ← DNA‑binding transcriptional dual regulator SgrR
RA 75,984 2 bp→CT coding (1315‑1316/1656 nt) sgrR ← DNA‑binding transcriptional dual regulator SgrR
RA 76,000 T→C K434E (AAA→GAA)  sgrR ← DNA‑binding transcriptional dual regulator SgrR
RA 76,082 T→C L406L (CTA→CTG sgrR ← DNA‑binding transcriptional dual regulator SgrR
RA 76,086 G→A T405I (ACC→ATC)  sgrR ← DNA‑binding transcriptional dual regulator SgrR
RA 76,283 C→T L339L (CTG→CTA sgrR ← DNA‑binding transcriptional dual regulator SgrR
RA 76,334 G→A G322G (GGC→GGT sgrR ← DNA‑binding transcriptional dual regulator SgrR
RA 76,358 C→A G314G (GGG→GGT sgrR ← DNA‑binding transcriptional dual regulator SgrR
RA 76,364 G→A A312A (GCC→GCT sgrR ← DNA‑binding transcriptional dual regulator SgrR
RA 76,478 A→G I274I (ATT→ATC sgrR ← DNA‑binding transcriptional dual regulator SgrR
RA 76,576 G→A L242L (CTG→TTG)  sgrR ← DNA‑binding transcriptional dual regulator SgrR
RA 76,700 A→G D200D (GAT→GAC sgrR ← DNA‑binding transcriptional dual regulator SgrR
RA 76,727 G→A H191H (CAC→CAT sgrR ← DNA‑binding transcriptional dual regulator SgrR
RA 76,775 A→T I175I (ATT→ATA sgrR ← DNA‑binding transcriptional dual regulator SgrR
RA 76,862 G→A I146I (ATC→ATT sgrR ← DNA‑binding transcriptional dual regulator SgrR
RA 77,021 C→T L93L (CTG→CTA sgrR ← DNA‑binding transcriptional dual regulator SgrR
RA 77,027 A→G D91D (GAT→GAC sgrR ← DNA‑binding transcriptional dual regulator SgrR
RA 77,117 G→A R61R (CGC→CGT sgrR ← DNA‑binding transcriptional dual regulator SgrR
RA 77,776 C→A G52G (GGC→GGA setA → sugar exporter SetA
RA 78,406 A→T G262G (GGA→GGT setA → sugar exporter SetA
RA 78,520 C→A F300L (TTC→TTA setA → sugar exporter SetA
RA 78,524 A→C N302H (AAT→CAT)  setA → sugar exporter SetA
RA 78,533 A→C M305L (ATG→CTG)  setA → sugar exporter SetA
RA 78,761 G→A V381I (GTC→ATC)  setA → sugar exporter SetA
RA 79,040 A→G A138A (GCT→GCC leuD ← 3‑isopropylmalate dehydratase subunit LeuD
RA 80,529 C→G G112G (GGG→GGC leuC ← 3‑isopropylmalate dehydratase subunit LeuC
RA 82,633 T→G I299I (ATA→ATC leuA ← 2‑isopropylmalate synthase
RA 82,894 T→G G212G (GGA→GGC leuA ← 2‑isopropylmalate synthase
RA 82,909 C→T L207L (TTG→TTA leuA ← 2‑isopropylmalate synthase
RA 82,933 G→A I199I (ATC→ATT leuA ← 2‑isopropylmalate synthase
RA 82,957 C→T V191V (GTG→GTA leuA ← 2‑isopropylmalate synthase
RA 83,179 C→T E117E (GAG→GAA leuA ← 2‑isopropylmalate synthase
RA 83,182 G→A D116D (GAC→GAT leuA ← 2‑isopropylmalate synthase
RA 83,296 T→C E78E (GAA→GAG leuA ← 2‑isopropylmalate synthase
RA 83,317 A→G C71C (TGT→TGC leuA ← 2‑isopropylmalate synthase
RA 83,344 G→A A62A (GCC→GCT leuA ← 2‑isopropylmalate synthase
RA 83,359 C→T S57S (TCG→TCA leuA ← 2‑isopropylmalate synthase
RA 83,371 G→A G53G (GGC→GGT leuA ← 2‑isopropylmalate synthase
RA 83,383 G→A V49V (GTC→GTT leuA ← 2‑isopropylmalate synthase
RA 84,251 G→T intergenic (‑543/‑117) leuL ← / → leuO leu operon leader peptide/DNA‑binding transcriptional dual regulator LeuO
RA 84,267 G→C intergenic (‑559/‑101) leuL ← / → leuO leu operon leader peptide/DNA‑binding transcriptional dual regulator LeuO
RA 84,305 2 bp→CG intergenic (‑597/‑62) leuL ← / → leuO leu operon leader peptide/DNA‑binding transcriptional dual regulator LeuO
RA 84,415 A→G K16K (AAA→AAG leuO → DNA‑binding transcriptional dual regulator LeuO
RA 85,481 T→C R11G (AGA→GGA)  yabR ← protein YabR
RA 86,241 A→G V204V (GTA→GTG ilvI → acetolactate synthase/acetohydroxybutanoate synthase, catalytic subunit
RA 86,271 C→T G214G (GGC→GGT ilvI → acetolactate synthase/acetohydroxybutanoate synthase, catalytic subunit
RA 86,315 C→T T229M (ACG→ATG)  ilvI → acetolactate synthase/acetohydroxybutanoate synthase, catalytic subunit
RA 86,345 G→C C239S (TGC→TCC)  ilvI → acetolactate synthase/acetohydroxybutanoate synthase, catalytic subunit
RA 86,658 A→G P343P (CCA→CCG ilvI → acetolactate synthase/acetohydroxybutanoate synthase, catalytic subunit
RA 87,410 C→T R18R (CGC→CGT ilvH → acetolactate synthase/acetohydroxybutanoate synthase, regulatory subunit
RA 89,089 C→A intergenic (+57/‑545) cra → / → mraZ DNA‑binding transcriptional dual regulator Cra/DNA‑binding transcriptional repressor MraZ
RA 89,103 G→C intergenic (+71/‑531) cra → / → mraZ DNA‑binding transcriptional dual regulator Cra/DNA‑binding transcriptional repressor MraZ
RA 89,143 A→G intergenic (+111/‑491) cra → / → mraZ DNA‑binding transcriptional dual regulator Cra/DNA‑binding transcriptional repressor MraZ
RA 89,145 G→T intergenic (+113/‑489) cra → / → mraZ DNA‑binding transcriptional dual regulator Cra/DNA‑binding transcriptional repressor MraZ
RA 89,239 G→A intergenic (+207/‑395) cra → / → mraZ DNA‑binding transcriptional dual regulator Cra/DNA‑binding transcriptional repressor MraZ
RA 89,241 C→A intergenic (+209/‑393) cra → / → mraZ DNA‑binding transcriptional dual regulator Cra/DNA‑binding transcriptional repressor MraZ
RA 89,263 A→G intergenic (+231/‑371) cra → / → mraZ DNA‑binding transcriptional dual regulator Cra/DNA‑binding transcriptional repressor MraZ
RA 89,316 T→C intergenic (+284/‑318) cra → / → mraZ DNA‑binding transcriptional dual regulator Cra/DNA‑binding transcriptional repressor MraZ
RA 89,321 A→G intergenic (+289/‑313) cra → / → mraZ DNA‑binding transcriptional dual regulator Cra/DNA‑binding transcriptional repressor MraZ
RA 89,471 T→G intergenic (+439/‑163) cra → / → mraZ DNA‑binding transcriptional dual regulator Cra/DNA‑binding transcriptional repressor MraZ
RA 89,570 A→C intergenic (+538/‑64) cra → / → mraZ DNA‑binding transcriptional dual regulator Cra/DNA‑binding transcriptional repressor MraZ
RA 89,699 T→C Y22Y (TAT→TAC mraZ → DNA‑binding transcriptional repressor MraZ
RA 89,754 T→C Y41H (TAT→CAT)  mraZ → DNA‑binding transcriptional repressor MraZ
RA 90,432 A→G E113E (GAA→GAG rsmH → 16S rRNA m(4)C1402 methyltransferase
RA 90,435 T→C R114R (CGT→CGC rsmH → 16S rRNA m(4)C1402 methyltransferase
RA 91,904 G→A E164E (GAG→GAA ftsI → peptidoglycan DD‑transpeptidase FtsI
RA 91,916 C→T Y168Y (TAC→TAT ftsI → peptidoglycan DD‑transpeptidase FtsI
RA 91,922 G→A P170P (CCG→CCA ftsI → peptidoglycan DD‑transpeptidase FtsI
RA 91,949 C→T I179I (ATC→ATT ftsI → peptidoglycan DD‑transpeptidase FtsI
RA 92,096 A→T A228A (GCA→GCT ftsI → peptidoglycan DD‑transpeptidase FtsI
RA 92,162 C→T N250N (AAC→AAT ftsI → peptidoglycan DD‑transpeptidase FtsI
RA 92,327 G→T P305P (CCG→CCT ftsI → peptidoglycan DD‑transpeptidase FtsI
RA 92,354 A→G V314V (GTA→GTG ftsI → peptidoglycan DD‑transpeptidase FtsI
RA 92,687 A→G P425P (CCA→CCG ftsI → peptidoglycan DD‑transpeptidase FtsI
RA 92,810 T→C T466T (ACT→ACC ftsI → peptidoglycan DD‑transpeptidase FtsI
RA 92,843 A→G P477P (CCA→CCG ftsI → peptidoglycan DD‑transpeptidase FtsI
RA 94,278 G→A A371A (GCG→GCA murE → UDP‑N‑acetylmuramoyl‑L‑alanyl‑D‑glutamate‑‑2, 6‑diaminopimelate ligase
RA 246,747 G→T S12S (TCG→TCT yafL → NlpC/P60 family protein YafL
MC JC 257,908 Δ776 bp insB9[crl] insB9, insA9, [crl]
MC JC 401,670 Δ728 bp [phoA] [phoA]
RA 480,295 2 bp→AA coding (13‑14/219 nt) hha ← hemolysin expression‑modulating protein Hha
JC JC 536,576 IS1 (+) +9 bp intergenic (+90/‑2) hyi → / → glxR hydroxypyruvate isomerase/tartronate semialdehyde reductase 2
RA 553,161 G→A intergenic (‑61/+57) purE ← / ← lpxH N(5)‑carboxyaminoimidazole ribonucleotide mutase/UDP‑2,3‑diacylglucosamine diphosphatase
RA 690,948 G→A V279V (GTC→GTT ybeX ← CorC‑HlyC family protein YbeX
RA 700,038 G→A H97Y (CAT→TAT)  umpH ← UMP phosphatase
JC JC 789,812 IS2 (–) +5 bp coding (164‑168/1149 nt) galK ← galactokinase
RA 791,688 G→A S123F (TCC→TTC)  galE ← UDP‑glucose 4‑epimerase
RA 809,217 2 bp→AG coding (40‑41/1290 nt) bioA ← adenosylmethionine‑8‑amino‑7‑oxononanoate aminotransferase
RA 968,837 G→A D158N (GAT→AAT)  lpxK → tetraacyldisaccharide 4'‑kinase
RA 1,040,126 G→T L277L (CTG→CTT appB → cytochrome bd‑II subunit 2
RA 1,061,935 G→A S46N (AGC→AAC)  torD → trimethylamine‑N‑oxide reductase‑specific chaperone
JC JC 1,093,481 IS2 (+) +5 bp coding (606‑610/1359 nt) dgcT → putative diguanylate cyclase DgcT
RA 1,100,070 G→A C144Y (TGT→TAT)  ycdY → chaperone protein YcdY
RA 1,111,727 2 bp→TT coding (865‑866/2544 nt) opgH → osmoregulated periplasmic glucans biosynthesis protein H
RA 1,123,556 C→T E167K (GAA→AAA)  grxB ← reduced glutaredoxin 2
RA 1,169,836 A→G L180P (CTG→CCG)  ldtC ← L,D‑transpeptidase LdtC
RA 1,189,980 A→G T156T (ACT→ACC phoP ← DNA‑binding transcriptional dual regulator PhoP
RA 1,196,220 C→T H366H (CAC→CAT icd → isocitrate dehydrogenase
RA 1,196,232 C→T T370T (ACC→ACT icd → isocitrate dehydrogenase
RA 1,196,245 T→C L375M (TTA→CTG)  icd → isocitrate dehydrogenase
RA 1,196,247 A→G L375M (TTA→CTG icd → isocitrate dehydrogenase
RA 1,196,277 C→T N385N (AAC→AAT icd → isocitrate dehydrogenase
RA 1,196,280 G→C A386A (GCG→GCC icd → isocitrate dehydrogenase
RA 1,196,283 A→G K387K (AAA→AAG icd → isocitrate dehydrogenase
MC JC 1,299,499 Δ1,199 bp insH21 insH21
RA 1,301,992 A→T N271Y (AAT→TAT)  oppA → oligopeptide ABC transporter periplasmic binding protein
RA 1,306,736 T→G S325A (TCC→GCC)  oppF → murein tripeptide ABC transporter/oligopeptide ABC transporter ATP binding subunit OppF
RA 1,310,770 A→G H32H (CAT→CAC yciI ← protein YciI
RA 1,331,947 C→T G300G (GGC→GGT topA → DNA topoisomerase 1
RA 1,337,394 A→G S522G (AGC→GGC)  acnA → aconitate hydratase 1
RA 1,357,202 C→T intergenic (‑92/+221) sapA ← / ← ymjA putative periplasmic binding protein SapA/DUF2543 domain‑containing protein YmjA
RA 1,412,897 G→A L113L (CTC→CTT intR ← putative integrase
RA 1,428,087 C→T E214K (GAG→AAG)  insH5 ← IS5 family transposase and trans‑activator
RA 1,610,076 T→G D202A (GAT→GCT)  uxaB ← tagaturonate reductase
RA 1,610,440 C→T E81K (GAA→AAA)  uxaB ← tagaturonate reductase
RA 1,643,679 A→T L209Q (CTG→CAG)  ydfU ← protein YdfU
JC JC 1,651,851 IS2 (–) +5 bp intergenic (+1866/+873) ydfD → / ← ynfP lysis protein/protein YnfP
RA 1,652,331 T→C intergenic (+2346/+397) ydfD → / ← ynfP lysis protein/protein YnfP
MC JC 1,704,350 Δ390 bp blr blr
RA 1,721,136 C→T L38F (CTT→TTT)  ydhI → DUF1656 domain‑containing protein YdhI
RA 1,738,377 C→T H178H (CAC→CAT purR → DNA‑binding transcriptional repressor PurR
RA 1,776,715 A→T S377C (AGT→TGT)  ydiF → putative acetate‑CoA transferase YdiF
RA 1,856,968 T→A I268F (ATT→TTT)  ydjI ← L‑glycero‑L‑galacto‑octuluronate‑1‑phosphate aldolase
RA 1,894,839 T→C L12P (CTC→CCC)  pabB → aminodeoxychorismate synthase subunit 1
RA 1,975,224 G→A A34V (GCG→GTG)  cheA ← chemotaxis protein CheA
RA 1,987,499 C→A intergenic (+71/+8) ftnB → / ← yecJ putative ferritin‑like protein/DUF2766 domain‑containing protein YecJ
RA 2,003,276 A→C S111A (TCT→GCT)  fliC ← flagellar filament structural protein
RA 2,003,462 C→A A49S (GCG→TCG)  fliC ← flagellar filament structural protein
RA 2,003,470 G→C A46G (GCG→GGG)  fliC ← flagellar filament structural protein
RA 2,040,433 C→A A319D (GCC→GAC)  msrP → protein‑L‑methionine sulfoxide reductase catalytic subunit MsrP
RA 2,173,363 Δ2 bp intergenic (‑490/+918) gatD ← / ← gatB galactitol‑1‑phosphate 5‑dehydrogenase/galactitol‑specific PTS enzyme IIB component
RA 2,175,991 T→A K111* (AAG→TAG)  gatZ ← putative tagatose‑1,6‑bisphosphate aldolase 2 chaperone
RA 2,188,087 T→C T110A (ACG→GCG)  yehA ← putative fimbrial adhesin YehA
JC 2,238,416 Δ19 bp coding (856‑874/1521 nt) mglA ← D‑galactose/methyl‑galactoside ABC transporter ATP binding subunit
MC JC 2,263,489 Δ3,371 bp [fruB][yeiQ] [fruB], setB, yeiW, yeiP, [yeiQ]
RA 2,302,355 G→T T133T (ACC→ACA napA ← periplasmic nitrate reductase subunit NapA
RA 2,384,808 C→T K305K (AAG→AAA yfbK ← IPR002035/DUF3520 domain‑containing protein YfbK
RA 2,410,419 G→A L312L (CTG→TTG)  yfbS ← putative transporter YfbS
RA 2,484,827 C→T L152F (CTT→TTT)  evgS → sensor histidine kinase EvgS
RA 2,700,747 G→A L84L (CTC→CTT acpS ← holo‑[acyl‑carrier‑protein] synthase
RA 2,748,396 G→A A145A (GCG→GCA ypjD → cytochrome c assembly family protein
RA 2,756,976 G→A R273Q (CGA→CAA)  intA → integrase IntA
RA 2,780,060 G→A H889H (CAC→CAT ypjA ← adhesin‑like autotransporter YpjA
RA 2,792,209 G→A V313M (GTG→ATG)  gabD → succinate‑semialdehyde dehydrogenase (NADP(+)) GabD
RA 2,800,435 G→A G97D (GGT→GAT)  ygaM → DUF883 domain‑containing protein YgaM
RA 2,810,925 G→A G52G (GGG→GGA mprA → DNA‑binding transcriptional regulator MprA
RA 2,817,850 G→A noncoding (11/77 nt) argQ ← tRNA‑Arg
RA 2,825,328 G→A S83S (TCC→TCT mltB ← membrane‑bound lytic murein transglycosylase B
RA 2,841,238 G→A M83I (ATG→ATA ascB → 6‑phospho‑beta‑glucosidase AscB
RA 2,863,773 C→T L61F (CTT→TTT)  ygbL → putative 3‑oxo‑tetronate 4‑phosphate decarboxylase YgbL
RA 2,878,077 C→T intergenic (+459/+492) iap → / ← cas2 alkaline phosphatase isozyme conversion protein/CRISPR‑associated endoribonuclease Cas2
RA 2,889,365 C→T K245K (AAG→AAA cysI ← sulfite reductase, hemoprotein subunit
RA 2,905,825 C→T Q39* (CAA→TAA)  ygcG → TPM domain‑containing protein YgcG
JC JC 2,913,392 IS2 (–) +5 bp coding (256‑260/2235 nt) relA ← GDP/GTP pyrophosphokinase
RA 2,926,292 C→T intergenic (+96/‑16) queF → / → ppnN 7‑cyano‑7‑deazaguanine reductase/nucleotide 5'‑monophosphate nucleosidase
RA 3,088,010 2 bp→TT intergenic (+150/‑273) metK → / → galP methionine adenosyltransferase/galactose:H(+) symporter
RA 3,336,411 2 bp→AT coding (83‑84/1260 nt) murA ← UDP‑N‑acetylglucosamine 1‑carboxyvinyltransferase
RA 3,367,944 G→A A40T (GCG→ACG)  yhcG → DUF1016 domain‑containing protein YhcG
RA 3,388,041 T→G T50P (ACG→CCG)  aaeB ← aromatic carboxylic acid efflux pump subunit AaeB
RA 3,399,929 A→T L17Q (CTG→CAG)  mreC ← cell shape determining protein MreC
RA 3,436,697 G→A E60E (GAG→GAA trkA → NAD‑binding component of Trk potassium transporters
RA 3,449,779 G→A S35S (AGC→AGT rpsC ← 30S ribosomal subunit protein S3
RA 3,468,768 C→T W362* (TGG→TGA chiA ← endochitinase
RA 3,474,425 T→C K43R (AAA→AGA)  rpsL ← 30S ribosomal subunit protein S12
RA 3,543,877 G→A P237P (CCG→CCA rpnA → recombination‑promoting nuclease RpnA
RA 3,560,455 +G intergenic (+589/+167) rtcR → / ← glpG DNA‑binding transcriptional activator RtcR/rhomboid protease GlpG
RA 3,629,921 C→T E228K (GAG→AAG)  yhiI ← putative membrane fusion protein YhiI
RA 3,682,515 C→T M311I (ATG→ATA dctA ← C4 dicarboxylate/orotate:H(+) symporter
RA 3,696,909 G→A G151E (GGA→GAA)  bcsE → c‑di‑GMP‑binding protein BcsE
RA 3,707,947 C→A intergenic (‑242/+669) dppA ← / ← proK dipeptide ABC transporter periplasmic binding protein/tRNA‑Pro
RA 3,725,176 T→G E48A (GAG→GCG)  glyQ ← glycine‑‑tRNA ligase subunit alpha
RA 3,761,230 G→A S7F (TCC→TTC)  selA ← selenocysteine synthase
RA 3,771,979 G→T intergenic (‑235/‑302) yibI ← / → mtlA DUF3302 domain‑containing protein YibI/mannitol‑specific PTS enzyme IICBA component
RA 3,815,883 +C coding (667/687 nt) rph ← truncated RNase PH
JC 3,822,651 (CAGGAT)1→2 coding (252/2109 nt) spoT → bifunctional (p)ppGpp synthase/hydrolase SpoT
RA 3,823,162 C→T H255Y (CAC→TAC)  spoT → bifunctional (p)ppGpp synthase/hydrolase SpoT
RA 3,869,637 C→A L87L (CTC→CTA cbrA → colicin M resistance protein
RA 3,878,536 G→A Y528Y (TAC→TAT gyrB ← DNA gyrase subunit B
RA 3,883,678 G→A P18S (CCA→TCA)  dnaA ← chromosomal replication initiator protein DnaA
RA 3,912,074 G→A S532F (TCC→TTC)  glmS ← L‑glutamine‑‑D‑fructose‑6‑phosphate aminotransferase
RA 3,931,069 C→T E9K (GAA→AAA)  ravA ← regulatory ATPase RavA
JC JC 3,938,983 IS2 (–) +5 bp coding (757‑761/993 nt) rbsR → DNA‑binding transcriptional dual regulator RbsR
RA 3,955,304 C→T R609R (CGC→CGT ilvD → dihydroxy‑acid dehydratase
RA 3,988,115 C→T D318N (GAT→AAT)  hemX ← PF04375 family protein HemX
RA 3,993,531 T→C R793R (CGT→CGC cyaA → adenylate cyclase
RA 3,996,049 T→A L156* (TTG→TAG)  yigA → DUF484 domain‑containing protein YigA
RA 4,011,802 C→T P243S (CCG→TCG)  bioP → biotin transporter
RA 4,084,903 T→G N307T (AAC→ACC)  fdoG ← formate dehydrogenase O subunit alpha
RA 4,092,703 A→G F41L (TTC→CTC)  frvA ← putative PTS enzyme IIA component FrvA
RA 4,113,851 G→A H208Y (CAT→TAT)  fpr ← flavodoxin/ferredoxin‑NADP(+) reductase
RA 4,124,401 G→T A19E (GCA→GAA)  cytR ← DNA‑binding transcriptional repressor CytR
RA 4,143,377 C→T S283L (TCA→TTA)  frwC → putative PTS enzyme IIC component FrwC
RA 4,154,371 G→A A159A (GCC→GCT argE ← acetylornithine deacetylase
RA 4,161,248 G→T G61V (GGC→GTC)  fabR → DNA‑binding transcriptional repressor FabR
RA 4,164,123 C→G A162G (GCA→GGA)  btuB → cobalamin outer membrane transporter
RA 4,215,347 T→A intergenic (+138/‑131) metA → / → aceB homoserine O‑succinyltransferase/malate synthase A
RA 4,223,638 T→C intergenic (‑10/‑190) iclR ← / → metH DNA‑binding transcriptional repressor IclR/cobalamin‑dependent methionine synthase
RA 4,226,699 G→A G958R (GGG→AGG)  metH → cobalamin‑dependent methionine synthase
RA 4,282,332 (A)5→6 coding (744/1293 nt) yjcF ← pentapeptide repeat‑containing protein YjcF
JC JC 4,286,247 IS30 (+) +2 bp coding (1124‑1125/1959 nt) acs ← acetyl‑CoA synthetase (AMP‑forming)
RA 4,293,781 2 bp→AT coding (239‑240/597 nt) nrfG → putative formate‑dependent nitrite reductase complex subunit NrfG
RA 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO
RA 4,325,644 C→T S33N (AGC→AAC)  yjdN ← PF06983 family protein YjdN
RA 4,392,997 G→A V357V (GTC→GTT queG ← epoxyqueuosine reductase
RA 4,393,552 G→A F172F (TTC→TTT queG ← epoxyqueuosine reductase
RA 4,396,373 C→T T103T (ACC→ACT amiB → N‑acetylmuramoyl‑L‑alanine amidase B
RA 4,396,772 T→C F236F (TTT→TTC amiB → N‑acetylmuramoyl‑L‑alanine amidase B
RA 4,425,688 C→A H57N (CAC→AAC)  priB → primosomal replication protein N
RA 4,425,696 C→A N59K (AAC→AAA priB → primosomal replication protein N
RA 4,499,661 T→C intergenic (+161/‑1599) insD6 → / → yjgZ IS2 insertion element protein InsB/uncharacterized protein YjgZ
RA 4,510,238 T→G intergenic (+445/+452) insO → / ← fecE IS911B regulator fragment/ferric citrate ABC transporter ATP binding subunit
RA 4,532,409 T→G intergenic (‑758/+28) sgcX ← / ← yjhP putative endoglucanase with Zn‑dependent exopeptidase domain/putative methyltransferase YjhP
JC JC 4,543,608 Δ1 bp :: IS186 (–) +6 bp :: Δ1 bp coding (494‑499/549 nt) fimA → type 1 fimbriae major subunit
RA 4,546,601 C→T I502I (ATC→ATT fimD → type I fimbriae usher protein
RA 4,636,925 G→C R77P (CGC→CCC)  creC → sensory histidine kinase CreC
RA 4,638,240 G→A L21L (TTG→TTA creD → putative inner membrane protein CreD

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 275509 278399 2891 23 [0] [8] 9 [mmuP]–[afuC] [mmuP],mmuM,[afuC]
* * ÷ NC_000913 278424 372748 94325 9 [8] [6] 29 [afuC]–[mhpD] [afuC],insB2,insA2,yagB,yagA,yagE,yagF,yagG,yagH,xynR,argF,argL,ykgS,insB3,insA3,yagJ,yagK,yagL,yagM,ykgV,yagN,intF,yagP,paoD,paoC,paoB,paoA,yagU,ykgJ,ecpE,ecpD,ecpC,ecpB,ecpA,matA,ykgL,ykgO,ykgM,ykgR,insE1,insF1,rclC,rclB,rclA,rclR,ykgE,ykgF,ykgG,ykgH,betA,betB,betI,betT,yahV,pdeL,yahB,yahC,yahD,yahE,yahF,yahG,yahH,yahI,yahJ,yahK,yahL,yahM,yahN,yahO,prpR,prpB,prpC,prpD,prpE,codB,codA,cynR,cynT,cynS,cynX,lacA,lacY,lacZ,lacI,mhpR,mhpA,mhpB,mhpC,[mhpD]
* * ÷ NC_000913 1196292 1211569 15278 10 [8] [8] 9 [icd]–mcrA [icd],C0293,ymfD,ymfE,lit,intE,xisE,ymfH,ymfI,ymfJ,ymfK,ymfT,ymfL,ymfM,ymfN,ymfR,beeE,ymfQ,ycfK,tfaP,tfaE,pinE,mcrA
* * ÷ NC_000913 1978495 1979270 776 24 [0] [3] 27 insB5–insA5 insB5,insA5
* * ÷ NC_000913 3423825–3424233 3424440–3424238 6–616 9 [8] [8] 9 [rrlD] [rrlD]
* * ÷ NC_000913 3708423 3708539 117 9 [8] [8] 9 dppA/proK dipeptide ABC transporter periplasmic binding protein/tRNA‑Pro

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 2755080 (0.000)23 (0.560) 21/466 0.9 95.8% coding (184/1404 nt) mmuP S‑methyl‑L‑methionine transporter
?NC_000913 372749 = 2 (0.050)coding (635/810 nt) mhpD 2‑hydroxypentadienoate hydratase
* ? NC_000913 = 1397238NA (NA)24 (0.560) 23/486 0.8 100% noncoding (1195/1195 nt) IS5 repeat region
?NC_000913 = 1978494 0 (0.000)intergenic (‑297/+24) flhD/insB5 DNA‑binding transcriptional dual regulator FlhD/IS1 family protein InsB
* ? NC_000913 1979271 =3 (0.070)24 (0.560) 23/486 0.8 88.9% intergenic (‑56/‑482) insA5/uspC IS1 family protein InsA/universal stress protein C
?NC_000913 = 2102947 NA (NA)pseudogene (443/446 nt) wbbL interrupted rhamnosyltransferase WbbL