| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 90,432 | A→G | E113E (GAA→GAG) | rsmH → | 16S rRNA m(4)C1402 methyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 90,432 | 0 | A | G | 89.8% | 157.0 / 7.1 | 60 | E113E (GAA→GAG) | rsmH | 16S rRNA m(4)C1402 methyltransferase |
| Reads supporting (aligned to +/- strand): ref base A (2/4); new base G (26/27); total (28/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.73e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.99e-02 | |||||||||||
GGTCACTCACGTCTGATCCTCTCGCAGCTTGGCGAAGAGGGGCGTTTGCTGGCGATCGATCGCGACCCGCAGGCTATCGCCGTTGCGAAGACTATTGATGATCCGCGCTTCTCCATCATCC‑ACGGACCTTTCTCCGCGCTGGGCGA‑ATACGTTGCCGAGCGCGATCTTATCGGCAAGATCGACGGCATTCTCCTCGATCTTGGCGTCTCTTCACCGCAACTTGATGATGCTGAACGTGGCTTTTCCTTTATGCGCGATGGTCCGCTGGACATGCGTATGGACCCAACCCGTGGGCAGTCAGCCGCTGAATGGCTACAAACCGCAGAAGAAGCCGATATCGCCTGGGTATTGAAAACCTATGG‑TGAAGAGCGTTTTGCCAAACGCATTGCCCGCGCCATTGTCGAGCGTAACCGCGAACAGCCGATGAC‑CCGCACCAAAGAACTGGCGGAAGTCGTGGCTGCTGCAACGCCGGTGAA > NC_000913/90199‑90674 | ggtCACTCACGTGTGATCCGCTCGCAGCTTGGCGAAGAGGGGCGTTTGCTGGCGATCGATCGCGACCCGCAGGCTATCGCCGTTGCGAAGACTATTGATGATCCGCGCTTCTCCATCATCC‑ACGGACCTTTCTCCGCGCTGGGCGA‑ATACGTTGCCGAGCGCGATCTTATCGGCAAGATCGACGGCATTCTCCTCGATCTTGGCGTCTCTTCACCGCAACTTGATGATGCTGAACGTGGCTTTTCCTTTa < 1:220278/250‑1 (MQ=255) gaaccTCTCGCAGCTTGGCGAAGAGGGGCGTTTGCTGGCGATCGATCGCGACCCGCAGGCTATCGCCGTTGCGAAGACTATTGATGATCCGCGCTTCTCCATCATCC‑ACGGACCTTTCTCCGCGCTGGGCGA‑ATACGTTGCCGAGCGCGATCTTATCGGCAAGATCGACGGCATTCTCCTCGATCTTGGCGTCTCTTCACCGCAACTTGATGATGCTGAACGTGGCTTTTCCTTTATGCGCGATGGTcc < 2:110886/246‑1 (MQ=255) gATCCTCTCGCAGCTTGGCGAAGAGGGGCGTTTGCTGGCGATCGATCGCGACCCGCAGGCTATCGCCGTTGCGAAGACTATTGATGATCCGCGCTTCTCCATCATCC‑ACGGACCTTTCTCCGCGCTGGGCGA‑ATACGTTGCCGAGCGCGATCTTATCGGCAAGATCGACGGCATTCTCCTCGATCTTGGCGTCTCTTCACCGCAACTTGATGATGCTGCGCGCGGCTTTTCCTTTATGCGCGATGGTCCGc > 2:6167/1‑251 (MQ=255) tcGCAGCTTGGCGAAGAGGGGCGTTTGCTGGCGATCGATCGCGACCCGCAGGCTATCGCCGTTGCGAAGACTATTGATGATCCGCGCTTCTCCATCATCC‑ACGGACCTTTCTCCGCGCTGGGCGA‑ATACGTTGCCGAGCGCGATCTTATCGGCAAGATCGACGGCATTCTCCTCGATCTTGGCGTCTCTTCACCGCAACTTGATGATGCTGAGCGCGGCTTTTCCTTTATGCGCGATGGTCCGCTGGACAt > 1:293330/1‑251 (MQ=255) aaggggggCGTTTGTTGGCGATCGATCGCGACCCGCAGGCTATCGCCGTTGCGAAGACTATTGATGATCCGCGCTTCTCCATCATCC‑ACGGACCTTTCTCCGCTCTGTGCGA‑ATACGTTGCCGAGCGCGATCTTATCGGCAAGATCGACGGCATTCGCCTCGATCTTGGCGTCTCTTCACCGCAACGTGATGATGCTGAGCGCGGCTTTCCCTTTATGCGCGATGGTCGGCTGGACATGCGTATGGACCCa < 2:60493/247‑1 (MQ=255) ggggCGTTTGCTGGCGATCGATCGCGACCCGCAGGCTATCGCCGTTGCGAAGACTATTGATGATCCGCGCTTCTCCATCATCC‑ACGGACCTTTCTCCGCGCTGGGCGA‑ATACGTTGCCGAGCGCGATCTTATCGGCAAGATCGACGGCATTCTCCTCGATCTTGGCGTCTCTTCACCGCAACTTGATGATGCTGAGCGCGGCTTTTCCTTTATGCGCGATGGTCCGCTGGACATGCGTATGGACCCAAccc > 1:404487/1‑251 (MQ=255) ggCGTTTGCTGGCGATCGATCGCGACCCGCAGGCTATCGCCGTTGCGAAGACTATTGATGATCCGCGCTTCTCCATCATCC‑ACGGACCTTTCTCCGCGCTGGGCGA‑ATACGTTGCCGAGCGCGATCTTATCGGCAAGATCGACGGCATTCTCCTCGATCTTGGCGTCTCTTCACCGCAACTTGATGATGCTGAGCGCGGCTTTTCCTTTATGCGCGATGGTCCGCTGGACATGCGTATGGACCCAACCCgg > 1:256750/1‑250 (MQ=255) cGTTTGCTGGCGATCGATCGCGACCCGCAGGCTATCGCCGTTGCGAAGACTATTGATGATCCGCGCTTCTCCATCATCC‑ACGGACCTTTCTCCGCGCTGGGCGA‑ATACGTTGCCGAGCGCGATCTTATCGGCAAGATCGACGGCATTCTCCTCGATCTTGGCGTCTCTTCACCGCAACTTGATGATGCTGAGCGCGGCTTTTCCTTTATGCGCGATGGTCCGCTGGACATGCGTATGGACCCAACCCGTgg > 1:249534/1‑251 (MQ=255) gTTTGCTGGCGATCGATCGCGACCCGCAGGCTATCGCCGTTGCGAAGACTATTGATGATCCGCGCTTCTCCATCATCC‑ACGGACCTTTCTCCGCGCTGGGCGA‑ATACGTTGCCGAGCGCGATCTTATCGGCAAGATCGACGGCATTCTCCTCGATCTTGGCGTCTCTTCACCGCAACTTGATGATGCTAAGCGCGGCTTTTCCTTTATGCGCGATGGTCCGCTGGACATGCGTATGGACCCAACCCGTggg > 1:42024/1‑251 (MQ=255) ggCGATCGATCGCGACCAGCAGGCTCTCGCCGTTGCGAAGACTAGTTATGATCCGCGCTTCTCCATCATCC‑ACGGACCTTTCTCCGCGCTGGGCGA‑ATACGTTGCCGATCGCGATCTTATCGGCAAGATCGACGGCATTCTCCTCGATCTTGGCGTCTCTTCACCGCAACTTGATGATGCTGAGCGCGGCTTTTCCTTTATGCGCGATGGTCCGCTGGACATGCGTATGGACCCAACCCGTGGGCAGTCAg < 2:13584/251‑1 (MQ=255) gcgACCCGCAGGCTATCGCCGTTGCGAAGACTATTGATGATCCGCGCTTCTCCATCATCC‑ACGGACCTTTCTCCGCGCTGGGCGA‑ATACGTTGCCGAGCGCGATCTTATCGGCAAGATCGACGGCATTCTCCTCGATCTTGGCGTCTCTTCACCGCAACTTGATGATGCTGAGCGCGGCTTTTCCTTTATGCGCGATGGTCCGCTGGACATGCGTATGGACCCAACCCGTGGGCAGTCAGCCGCTGAATgg < 1:222334/251‑1 (MQ=255) gtcccGCAGGCTATCGCCGGTGCGAAGACTATTGATTATCCTCGCTTCGCCATCATCC‑ACGGACCTTTCTCCGCGCTGGGCGA‑ATTCGTTGCCGAGCGCGATCTTATCGGCAAGATCGACGGCATTCTCCTCGATCTTGGCGTCTCTTCACCGCAACTTGATGATGCTGAGCGCGGCTTTTCCTTTATGCGCGATGGTCCGCTGGACATGCGTATGGACCCATCCCGTGGGCAGTCAGTCGCTGAATGGCt < 1:381019/249‑1 (MQ=255) aGGCTATCGCCGTTGCGAAGACTATTGATGATCCGCGCTTCTCCATCATCC‑ACGGACCTTTCTCCGCGCTGGGCGA‑ATACGTTGCCGAGCGCGTTCTTATCGGCAAGATCGACGGCATTCTCCTCGATCTTGGCGTCTCTTCACCGCAACTTGATGATGCTGAGCGCGGCTTTTCCTTTATGCGCGATGGTCCGCTGGACATGCGTATGGACCCAACCCGTGGGCAGTCAGCCGCTGAATGGCTACAAAcc < 1:78181/251‑1 (MQ=255) cGCCGTTGCGAAGACTATTGATGATCCGCGCTTCTCCATCATCC‑ACGGACCTTTCTCCGCGCTGGGCGA‑ATACGTTGCCGAGCGCGATCTTATCGGCAAGATCGACGGCATTCTCCTCGATCTTGGCGTCTCTTCACCGCAACTTGATGATGCTGAGCGCGGCTTTTCCTTTATGCGCGATGGTCCGCTGGACATGCGTATGGACCCAACCCGTGGGCAGTCAGCCGCTGAATGGCTACAAACCGCaga > 1:324263/1‑249 (MQ=255) ggtgagAAGACTAGTGATGATCCGCGCTTCTCCATCATCC‑ACGGGCCTTTCTCCGCGCTGGGCGA‑ATACGTTGCCGAGCGCGATCTTAGCGGCAAGAGCGACGGCATTCTCCTCGATCTTGGCGTCTCTTCACCGCAACTTGATGATGCTGAGCGCGGCTTTTCCTTTATGCGCGATGGTCCGCTGGACATGCGTATGGACCCAACCCGTGGGCAGTCAGCCGCTGAATGGCTACAAACCGCAGAAGAAGc < 1:452712/246‑1 (MQ=255) tGCGAAGACTATTGATGATCCGCGCTTCGCCATCATCC‑ACGGACCTTTCTCCGCGCTGGGCGA‑ATACGTTGCCGAGCGCGATCTTATCGGCAAGATCGACGGCATTCTCCTCGATCTTGGCGTCTCTTCACCGCAACTTGATGATGCTGAGCGCGGCTGTTCCTGTATGCGCGATGGTCCGCTGGACATGCGTATGGACCCAACCCGTGGGCAGTCAGCCGCTGAATGGCTACAAACCGCAGAAGAAGcc < 1:24856/250‑1 (MQ=255) gAAGACTATTGATGATCCGCGCTTCTCCATCATCC‑ACGGACCTTTCTCCGCGCTGGGCGA‑ATACGTTGCCGAGCGCGATCTTATCGGCAAGATCGACGGCATTCTCCTCGATCTTGGCGTCTCTTCACCGCAACTTGATGATGCTGAGCGCGGCTTTTCCTTTATGCGCGATGGTCCGCTGGACATGCGTATGGACCCAACCCGTGGGCAGTCAGCCGCTGAATGGCGACAAACCGCAGAAGAAGACGat > 2:66812/1‑250 (MQ=255) gAAGACTATTGATGAGCCGCGCTTCTCCATCATCC‑ACGGACCTTTCTCCGCGCTGTGCGA‑ATACGTGGCCGAGCGTGATCTTATCGGCAAGACCGACGGCATTCTCCTCGATCTTGGCGTCTCTTCACCGCAACTTGATGATGCTGAGCGCGGCTTTTCCTTTATGCGCGATGGTCCGCTGGACATGCGTATGGACCCAACTCGTGTGCAGTCAGCCGCTGAATGGCTACAAACCGCAGAAGAAGCCGata < 2:256116/251‑1 (MQ=255) aGACTATTGATGATCCGCGCTTCTCCATCATCC‑ACGGACCTTTCTCCGCGCTGGGCGA‑ATACGTTGCCGAGCGCGATCTTATCGGCAAGATCGACGGCATTCTCCTCGATCTTGGCGTCTCTTCACCGCAACTTGATGCTGCTGAGCGCGGCTTTTCCTTTATGCGCGATGGTCCGCTGGACATGCGTATGGACCCAACCCGTGGGCAGTCAGCCGCTGAATGGCTACaaa > 2:208186/1‑231 (MQ=255) aGACTATTGATGATCCGCGCTTCTCCATCATCC‑ACGGACCTTTCTCCGCGCTGGGCGA‑ATACGTTGCCGAGCGCGATCTTATCGGCAAGATCGACGGCATTCTCCTCGATCTTGGCGTCTCTTCACCGCAACTTGATGATGCTGAGCGCGGCTTTTCCTTTATGCGCGATGGTCCGCTGGACATGCGTATGGACCCAACCCGTGGGCAGTCAGCCGCTGAATGGCTACaaa < 1:208186/231‑1 (MQ=255) aGACTATTGATGATCCGCGCTTCTCCATCATCC‑ACGGACCTTTCTCCGCGCTGGGCGA‑ATACGTTGCCGAGCGCGATCTTATCGGCAAGATCGACGGCATTCTCCTCGATCTTGGCGTCTCTTCACCGCAACTTGATGATGCTGAGCGCGGCTTTTCCTTTATGCGCGATGGTCCGCTGGACATGCGTATGGACCCAACCCGTGGGCAGTCAGCCGCTGAATGGCTACAAACCGCAGAAGAAGCCGAAATc > 2:445733/1‑251 (MQ=255) gACTATTGATGATCCGCGCTTCTCCATCATCC‑ACGGACCTTTCTCCGCGCTGGGCGA‑ATACGTTGCCGAGCGCGATCTTATCGGCAAGATCGACGGCATTCTCCTCGATCTTGGCGTCTCTTCACCGCAACTTGATGATGCTGAGCGCGGCTTTTCCTTTATGCGCGATGGTCCGCTGGACATGCGTATGGACCCAACCCGTGGGCAGTCAGCCGCTGAATGGCTACAAACCGCAGAAGAAGCCGATATCg < 1:73629/251‑1 (MQ=255) gatgatCCACGTTTCTCCATCATCCCACG‑AGCTTTTGCCGGGCGGGGCGA‑ATTCGTTTCCGAGCGCGATCTTATCGGCAAGATCGACGGCAGTCTCCGCGATCTTGGCGTCTCTTCACCTCAACTTGATGATGCTGACCGCGGCTTTTCCTTTATGCGCGATGGTCAGCTGGATAGGCGTATGTACCCAACCCGTTGGCAGTCAGCCTCTGAATGGCTACATACCGCAGAAGAATCCGAGATCGCCTggt < 2:325289/250‑2 (MQ=255) gatCCGCGCTTCTCCATCATCC‑ACGGACCTTTCTCCGCGCTGGGCGA‑ATCCGTTGCCGAGCGCGATCTTATCGGCAAGATCGACGGCATTCTCCTCGATCTTGGCGTCTCTTCACCGCAACTTGATGATGCTGAACGTGGCTTTTCCTTTAGGCGCGATGGTCCGCTGGACATGCGTATGGACCCAACCCGTGGGCAGTCAGCCGCTGAATGGCTACAAACCGCAGAAGAAGCCGATATCGCCTGGGTAt < 2:8913/250‑1 (MQ=255) gatCCGCGCTTCTCCATCATAC‑ACGGACATTTCTCCGCGCTGGGCGA‑ATACGTTGCCGAGCGCGATCTTATCGGCAAGATCGACGGCATTCTCCTCGATCTTGGCGTCTCTTCACCGCACCTTGATGATGCTGAGCGCGGCTTTTCCTTTATGCGCGATGGTCCGCTGGACATGCGTATGGACCCAACCCGTGGGCAGTCAGCCGCTGAATGGCTACAAACCGAACAAGAAACCGGAATCGCCCGGGtctt > 2:25883/1‑248 (MQ=255) acccGCGCTTCTCCATCATCC‑GCGGACCTTCCCCCCCGGTGGGCGA‑ATACGTTGCCGAGCGCTATCTTAGCTGCAAGATCACCGGCATTCGCCTCGATCTTGGCGTCTCTTCACCGCAACTTGATTATGCTGAGCGCGGCTTTTCCTTTATGCGCGATGGTCCGCTGGACATGCGTATGGACCCAACCCGTGGGCAGTCAGCCGCTGAATGGCTACAAACCGCAGAAGAAGCCGATATCGCCTGGGTATTg < 2:293330/249‑1 (MQ=255) cgtgcgtccccaacagcc‑ACGGACCTTGCGCCGCGATGGGCGAGAT‑CGTGGCCGGGCTCGATCTTATCGGCAAGATCGACGGCATTCTCCTCGATCTTGGCGTCTCTTCACCGCAACTTGATGATGCTGAGCGCGGCTTTTCCTTTATGCGCGATGGTCCGCTGGACATGCGTATGGACCCAACCCGTGTGCAGTCAGCCGCTGAAGGGCTACAAACCGCAGAAGAAGCCGATATCGCCTGGGTATTGaaa < 2:222910/235‑1 (MQ=255) tctcCATCATCC‑ACGGTCCTTTCTCCGCGCTTGGCGA‑ATACGTTGCCGAGCGCGATCTTATCGGCAAGATCGACGGCATTCTCCTCGATCTTGGCGTCTCTTCACCGCAACTTGATGATGCTGAGCGCGGCTTTTCCTTTATGCGCGATGGTCCGCTGGACATGCGTATGGACCCAACCCGTGGGCAGTCAGCCGCTGAATGGCTACAAACCGCAGAAGAAGCCGATATCGCCTGGGTATTGAAAACCt < 1:19855/249‑1 (MQ=255) tctcCATCATCC‑ACGGACCTTTCTCCGCGCTGGGCGA‑ATACGTTGCCGAGCGCGATCTTAACGGCAAGATCGACGGCATTCTCCTCGATCTTGGCGTCTCTTCACCGCAACTTGATGATGCTGAGCGCGGCTTTTCCTTTATGCGCGATGGTCCGCTGGACATGCGTATGGACCCAACCCGTGGGCAGTCAGCCGCTGAATGGCTACAAACCGCAGAAGAAGCCTATATCGCCTTGGTATTGAAAACCTa < 1:329421/250‑1 (MQ=255) ccATCATCC‑CCGGACCTTTCTCCGCGCTGTGCGA‑ATACGTTGCCGAGCGCGATCTTATCGGCAAGATCGACAGCATTCTCCTCGCTCTTGGCGTCTCTTCACCGCAACTTGATGATGCTGAGCGCGGCTTTTCATTTCTGCGCGCTGGTCCGCTGGACATTCGTATGGACCCCACCCGTGGGCAGTCAGCCGCTGCATGGCTACAACCCGCCGAAACAACCGATAACGCATGGGTATTGacacccaatgtt > 2:244520/1‑240 (MQ=255) ccatcC‑ACGGACCTTTCTCCGCGCTGGGCGA‑ATACGTTGCCGAGCGCGATCTTATCGGCAAGATCGACGGCATTCTCCTCGATCTTGGCGTCTCTTCACCGCAACTTGATGATGCTGAGCGCGGCTTTTCCTTTATGCGCGATGGTCCGCTGGACATGCGTATGGACCCAACCCGTGGGCAGTCAGCCGCTGAATGGCTACAAACCGCAGAAGAAGCCGATATCGCCTGGGTATTGAAAACCTATGG‑TGaa < 1:445733/250‑1 (MQ=255) tcC‑ACGGACCTTTCTCCGCGCTGGGCGA‑ATACGTTGCCGAGCGCGATCTTATCGGCAAGATCGACGGCATTCTCCTCGATCTTGGCGTCTCTTCACCGCAACTTGATGATGCTGAGCGCGGCTTTTCCTTTATGCGCGATGGTCCGCTGGACATGCGTATGGACCCAACCCGTGGGCAGTCAGCCGCTGAATGGCTACAAACCGCAGAGGAAGCCGATATCGCCTGGGTATTGAAAACCTATGG‑TGAag > 2:356357/1‑249 (MQ=255) ccc‑ACGGACCTTTCTCCGCGCTGGGCGA‑ATACGTTGCCGAGCGCGATCTTATCGGCAAGATCGACGGCATTCTCCTCGATCTTGGCGTCTCTTCACCGCAACTTGATGATGCTGAGCGCGGCTTTTCCTTTATGCGCGATGGTCCGCTGGACATGCGTATGGACCCAACCCGTGGGCAGTCAGCCGCTGAAGGGCTACAAACCGCAGAAGAAGCCGATATCGCCTGGGTATTGAAAACCTATGG‑TGAagag < 1:361907/250‑1 (MQ=255) tccccGGACCTTTCTCCGCGCTGGGAGT‑ATCCGTGGCCGAGGGCGATCTTATCGGCAAGATCGACGGCTTGCTCCTCGATCTTGGCTTCTCTTCACCGCAATTTGATGATGCGGAGCGCGGCTTTTCCTTTAGTCGCGATGGTCCGCTGGACATGCGTATGGACCCAACCCGTGGGCAGTCAGCCGCTGAATGGCTACAAACCGCAGAAGAAGCCGATAGGGCCTGGGTATTGAAACCCTATTG‑TGAaga < 2:386207/246‑1 (MQ=255) ccTTTCTCCGCGCTGGGCGA‑ATACGTTGCCGAGCGCGATCTTATCGGCAAGATCGACGGCATTCTCCTCGATCTTGGCGTCTCTTCACCGCAACTTGATGATGCTGAGCGCGGCTTTTCCTTTATGCGCGATGGTCCGCTGGACATGCGTATGGACCCAACCCGTGGGCAGTCAGCCGCTGAATGGCTACAAACCGCAGAAGAAGCCGATATCGCCTGGGTGTTGAAAACCTATGG‑TGAAAAGCGTTTTGc > 2:230148/1‑251 (MQ=255) cTTTCTCCGCGCTGGGCGA‑ATACGTTGCCGAGCGCGATCTTATCGGCAAGATCGACGGCATTCTCCTCGATCTTGGCGTATCTTCACCGCAACTTGATGATGCTGAGCGCGGCTTTTCCTTTATGCGCGATGGTCCGCTGGACATGCGTATGGACCCAACCCGTGGGCAGTCAGCCGCTGAATGGCTACAAACCGCAGAAGAAGCCGATATCGCCTGGGTATTGAAAACCTATGG‑TGAAGAGCGTTTTGcc > 1:431671/1‑251 (MQ=255) gcTGGGCGT‑ATACGTTGCCGAGCGCGACCTTATCGGCAAGATCGAAGGAATTCTCCTCGATCTTGGCGTCTCTTCACCGCAACTTGATGATGCTGCGCGCGGCTTTTCCTTTATGCGCGTTGGTCCGATGGACATGCGTCTGGACCCAATCCGTGGGCAGTCAGCCGCTGAATGGCTACAAACCGCAGAAGCAGCCGATGTCGCCTGGGTATTGAAAACCTATGG‑TGACGAGCGTTTTGCCAAACGCATTg > 2:29608/1‑251 (MQ=255) aTACGTTGCCGAGCGCGATCTTATCGGCAAGATCGACGGCATTCTCCTCGATCTTGGCGTCTCTTCACCGCAACTTGATGATGCTGAGCGCGGCTTTTCCTTTATGCGCGATGGTCCGCTGGACATGCGTATGGACCCACCCCGTGGGCAGTCAGCCGCTGAATGGCTACCAACCGCCGAAGAAGCCGATATCGCCTGGGTATTGAAAACCTATGG‑TGAAGAGCGGTTTGCCaaagttcggcagagggtcg > 2:305440/1‑235 (MQ=255) aTACGTTGCCGAGCGCGATCTTATCGGCAAGATCGACGGCATTCTCCTCGATCTTGGCGTCTCTTCACCGCAACTTGATGATGCTGAGCGCGGCTTTTCCTTTATGCGCGATGGTCCGCTGGACATGCGTATGGACCCAACCCGTGGGCAGTCAGCCGCTGAATGGCTACAAACCGCAGAAGAAGCCGATATCGCCTGGGTATTGAAAACCTATGG‑TGAAGAGCGTTTTGCCaa < 1:305440/234‑1 (MQ=255) gTTGCCGAGCGCGATCTTATCGGCAAGATCGACGGCATTCTCCTCGATCTTGGCGTCTCTTCACCGCAACTTGATGATGCTGAGCGCGGCTTTTCCTTTATGCGCGATGGTCCGCTGGACATGCGTATGGACCCAACCCGTGGGCAGTCAGCCGCTGAATGGCTACAAACCGCAGAAGAAGCCGATATCGCCTGGGTATTGAAAACCTATGG‑TGAAGAGCGTTTTGCCAAACGCATTGCCCGCGCCATTGt < 1:417694/251‑1 (MQ=255) gcgcGATCTTATCGGCAAGATCGACGGCATTCTCCTCGATCTTGGCGGCTCTTCACCGCAACTTGATGAGGCTGAGCGCGGCTTTTCCTTTATGCGCGATGGTCCGATGGACATGCGTATGGACCCAACCCGTGGGCAGGCAGCCGCTGAATGGCTACAAACCGCAGAAGAAGCCGATATCGCCTGGGTATTGAAAACCTATGG‑TGAAGAGCGTTTTGCCAAACGCATTGCCCGCGCCATTGTCGAGCGTa < 1:197871/251‑1 (MQ=255) cTTATCGGCAAGATCGACGGCATTCTCCTCGATCTTGGCTTCTCTTCACCGCAACTTGATGATGCTGAGCGCGGCTTTTCCTTTATGCGCGATGGTCCGCTGGACATGCGTATGGACCCAACCCGTGGGCAGTCAGCCGCTGAATGGCTACAAACCGCAGAAGAAGCCGATATCGCCTGGGTATTGAAAACCTATGG‑TGAAGAGCGTTTTGCCAAACGCATTGCCCGCGCCATTGTCGAGCGTAACcgcg > 1:437006/1‑250 (MQ=255) taccGGCAAGATCGACGGCATTCTCCTCGATCTTGGCGTCTCTTCACCGCAACTTGATGATGCTGAGCGCGGCTTTTCCTTTATGCGCGATGGTCCGCTGGACATGCGTATGGACCCAACCCGTGGGCAGTCAGCCGCTGAATGGCTACAAACCGCAGAAGAAGCCGATATCGCCTGGGTATTGAAAACCTATGG‑TGAAGAGCGTTTTGCCAAACGCATTGCCCGCGCCATTGTCGAGCGTAACcgcg < 2:249534/245‑1 (MQ=255) cGGCAAGATCGACGGCATTCTCCTCGATCTTGGCGTCTCTTCACCGCAACTTGATGATGCTGAACGTGGCTTTTCCTTTATGCGCGATGGTCCGCTGGACATGCGTATGGACCCAACCCGTGG‑CAGTCAGCCGCTGAATGGCTACAAACCGCAGAAGAAGCCGATATCGCCTGGGTATTGAAAACCTATGG‑TGAAGAGCGTTTTGCCAAACGCATTGCCCGCGCCATTGTAGAGCGTAAACGCGAACAGcc > 1:207376/1‑251 (MQ=255) cGACGGCATTCTCCTCGATCTTGGCGTCTCTTCACCGCAACTTGATGATGCTGAACGTGGCTTTTCCTTTATGCGCGATGGTCCGCTGGACATGCGTATGGACCCAACCCGTGGGCAGTCAGCCGCTGAATGGCTACAAACCGCAGAAGAAGCCGATATCGCCTGGGTATTGAAAACCTATGG‑TGAAGAGCGTTTTGCCAAACGCATTGCCCGCGCCATTGTCGAGCGTAACCGCGAACAGCCGATGAc‑cc > 1:42316/1‑251 (MQ=255) aCGGCATTCTCCTCGATCTTGGCGTCTCTTCACCGCAACTTGATGATGCTGAGCGCGGCTTTTCCTTTATGCGCGATGGTCCGCTGGACATGCGTATGGACCCAACCCGTGGGCAGTCAGCCGCTGAATGGCTACAAACCGCAGAAGAAGCCGATATCGCCTGGGTATTGAAAACCTATGG‑TGAAGAGCGTTTTGCCAAACGCAtt > 2:467022/1‑206 (MQ=255) aCGGCATTCTCCTCGATCTTGGCGTCTCTTCACCGCAACTTGATGATGCTGAGCGCGGCTTTTCCTTTATGCGCGATGGTCCGCTGGACATGCGTATGGACCCAACCCGTGGGCAGTCAGCCGCTGAATGGCTACAAACCGCAGAAGAAGCCGATATCGCCTGGGTATTGAAAACCTATGG‑TGAAGAGCGTTTTGCCAAACGCAtt < 1:467022/206‑1 (MQ=255) cGGCATTCTCCTCGATCTTGGCGTCTCTTCACCGCAACTTGATGATGCTGAGCGCGGCTTTTCCTTTATGCGCGATGGTCCGCTGGACATGCGTATGGACCCAACCCGTGGGCAGTCAGCCGCTGAATGGCTACAAACCGCAGAAGCAGCCGATATCGCCTGGGTATTGAAAACCTATGG‑TGAAGAGCGTTTTGCCAACCGCATTGCCCGCGCCATTGTCGAGCGTAACCGCGAACAGCCGATGAC‑CCGcc > 2:47425/1‑250 (MQ=255) cGGCATTCTCCTCGATCTTGGCGTCTCTTCACCGCAACTTGATGATGCTGAGCGCGGCTTTTCCTTTATGCGCGATGGTCCGCTGGACATGCGTATGGACCCAACCCGTGGGCAGTCAGCCGCTGAATGGCTACAAACCGCAGAAGAAGCCGATATCGCCTGGGTATTGAAAACCTATGG‑TGAAGAGCGTTTTGCCAAACGCATTGCCCGCGCCATTGTCGAg > 1:132695/1‑223 (MQ=255) ggCATTCTCCTCGATCTTGGCGTCTCTTCACCGCAACTTGATGATGCTGAGCGCGGCTTTTCCTTTATGCGCGATGGTCCGCTGGACATGCGTATGGACCCAACCCGTGGGCAGTCAGCCGCTGAATGGCTACAACCCGCAGAAGAAGCCGATATCGCCTGGGTATTGAAAACCTATGGGTGA‑GAGCGTTTTGCCAAACGAATTGCCCGCGCCATTGTCGAGCAGAACCGCGAGCAGCCGATGAC‑CCGccc > 2:328069/1‑249 (MQ=255) cATTCTCCTCGATCTTGGCGTCTCTTCACCGCCACTTGATGATGCTGAGCGCGGCTTTTCCTTTATGCGCGATGGTCCGCTGGACATGCGTATGGACCCAACCCGTGGGCAGTCAGCCGCTGAATGGCTACAAACCGCAGAAGAAGCCGATATCGCCTGGGGATTGAAAACCTATGG‑TGAAGAGCGTTTTGCCAAACGCATTGCCCGCGCCATTGTCGAGCGTAACCGCGAACAGCCGATGAC‑CCGCAcc > 1:12630/1‑250 (MQ=255) aTTCTCCTCGATCTTGGCGTCTCTTCACCGCAACTTGATGATGCTGAGCGCGGCTTTTCCTTTATGCGCGATGGTCCGCTGGACATGCGTATGGACACAACCCGTGGGCAGTCCGCCGCTGAATGGCTACAAACCGCAGAAGAAGCCGATATCGCCTGGGTATTGAAAACCTATGG‑TGAAGAGCGTTTTGCCAAACGCATTGCCCGCGCCATTGTCGAGCGTAACCGCGAACAGCCGATGAC‑CCGCACCa > 1:53409/1‑250 (MQ=255) aTTCCCCTCGATCTTGGCGTCTCTTCACCGCAACTTGATGATGCTGAGCGCGGCTTTTCCTTTATGCGCGATGGTCCGCTGGACATGCGTATGGACCCAACCCGTGGGCAGTCAGCCGCTGAATGGCTACAAACCGCAAAAGCAGCCGATATCGCCTGGGTATTGAAAACCTATGG‑AGAAGAGCGTTTTGCCAAACGCATTGTCCGCGCCATTGTCGAGCGAAACGGCGAACAGCCGAT‑ACGCCGCACCa > 2:130713/1‑250 (MQ=255) gcttctcTTCACCGCAACGTGATGATGCTGAGCGCGTCTTTTCCTTTATGCGCGATGGTCCGCTGGACATGCGTATGGACCCAACCCGTGGGCAGTCAGCCGCTGAATGGCTACAAACCGCAGAAGAAGCCGATATCGCCTGGGTATTGAAAACCTATGG‑TGAAGAGCGTTTTGCCAAACGCATTGCCCGCGCCATTGTCGAGCGTAACCGCGAACAGCCGATGTC‑CCGCACCAAAGAACTGGCGGAAGTc < 1:377743/248‑1 (MQ=255) ttccccGCAACTTTATGATGCTGAGGGCGGCTTTTCCTTTATGCGCGAGGGTCCGCTGGACATGCGTATGGACCCAACCCGTGGGCAGTCAGCCGCTGAATGGCTACAAACCGCAGAAGAAGCCGATATCGCCTGGGTATTGAAAACCTATGG‑TGAAGAGCGTTTTGCCAAACGCATTGCCCGCGCCATTGTCGAGCGTAACCGCGAACAGCCGATGAC‑CCGCACCAAAGAACTGGCGGAAGTCGTGgct < 2:304597/246‑1 (MQ=255) tCACCGCAACTGGATGATGCGGGACGCGGCTTTTCCTTTAGGCGGGATGGCCCTCGGGACATGCTTAGGGCCCCAACCCGTGGGCAGTCAGCCGCTGAATGGCTACAAACCGCAGAAGAAGCCGATATCGCCTGGGTATTGAAAACCTATGG‑TGAAGAGCGTTTTGCCAAACGCATTGCCCGCGCCATTGTCGAGCGTAACCGCGAACAGTCGATGAC‑CCGCACCAAAGAACTGGCGGAAGGCGTGgc < 2:431671/248‑1 (MQ=255) tgatgaTGCTGAGCGCGGCTTTTCCTTTATGCGCGATGGTCCGCTGGACATGCGTATGGACCCAACCCGTGGGCAGTCAGCCGCTGAATGGCTACAAACCGCAGAAGAAGCCGATATCGCCTGGGTATTGAAAACCTATGG‑TGAAGAGCGTTTTGCCAAACGCATTGCCCGCGCCATTGTCGAGCGTAACCGCGAACAGCCGATGAC‑CCGCACCAAAGAACTGGCGGAAGTCGTGGCTGCTGCAACGcc < 1:296722/249‑1 (MQ=255) atgatgCTGAGCGCGGCTTTTCCTTTATGCGCGATGGTCCGCTGGACATGCGTATGGACCCAACCCGTGGGCAGTCAGCCGCTGAATGGCTACAAACCGCAGAAGAAGCCGATATCGCCTGGGTATTGAAAACCTATGG‑TGAAGAGCGTTTTGCCAAACGCATTGCCCGCGCCATTGTCGAGCGTAACCGCGAACAGCCGATGCC‑CCGCACCAAAGAACTGGCGGAAGTCGTGGCTGCTGCAACGCCGGTg < 1:66812/251‑1 (MQ=255) gatgCTGAGCGCGGCTTTTCCTTTATGCGCGATGGTCCGCTGGACATGCGTATGGACCCAACCCGTGGGCAGTCAGCCGCTGAATGGCTACAAACCGCAGAAGAAGCCGATATCGCCTGGGTATTGAAAACCTATGG‑TGAAGAGCGTTTTGCCAAACGCATTGCCCGCGCCATTGTCGAGCGTAACCGCGAACAGCCGATGAC‑CCGCACCAAAGAACTGGCGGAAGTCGTGGCTGCTGCAACGCCGGTGaa < 1:6167/251‑1 (MQ=255) gCTGAGCGCGGCTTTTCCTTTATGCGCGATGGTCCGCTGGACATGCGTATGGACCCAACCCGTGGGCAGTCAGCCGCTGAATGGCTACAAACCGCAGAAGAAGCCGATATCGCCTGGGTATTGAAAACCTATGG‑TGAAGAGCGTTTTGCCAAACGCATTGCCCGCGCCATTGTCGAGCGTAACCGCGAACAGCCGATGAC‑CCGCACCAAAGAACTGGCGGAAGTCGTGGCTGATGAAACGCCGGTGaa > 1:90846/1‑248 (MQ=255) | GGTCACTCACGTCTGATCCTCTCGCAGCTTGGCGAAGAGGGGCGTTTGCTGGCGATCGATCGCGACCCGCAGGCTATCGCCGTTGCGAAGACTATTGATGATCCGCGCTTCTCCATCATCC‑ACGGACCTTTCTCCGCGCTGGGCGA‑ATACGTTGCCGAGCGCGATCTTATCGGCAAGATCGACGGCATTCTCCTCGATCTTGGCGTCTCTTCACCGCAACTTGATGATGCTGAACGTGGCTTTTCCTTTATGCGCGATGGTCCGCTGGACATGCGTATGGACCCAACCCGTGGGCAGTCAGCCGCTGAATGGCTACAAACCGCAGAAGAAGCCGATATCGCCTGGGTATTGAAAACCTATGG‑TGAAGAGCGTTTTGCCAAACGCATTGCCCGCGCCATTGTCGAGCGTAACCGCGAACAGCCGATGAC‑CCGCACCAAAGAACTGGCGGAAGTCGTGGCTGCTGCAACGCCGGTGAA > NC_000913/90199‑90674 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |