Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 84,305 2 bp→CG intergenic (‑597/‑62) leuL ← / → leuO leu operon leader peptide/DNA‑binding transcriptional dual regulator LeuO

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00091384,3050TC93.9% 92.3 / ‑1.5 33intergenic (‑597/‑63)leuL/leuOleu operon leader peptide/DNA‑binding transcriptional dual regulator LeuO
Reads supporting (aligned to +/- strand):  ref base T (1/1);  new base C (21/10);  total (22/11)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.82e-01
*NC_00091384,3060CG93.9% 100.9 / 0.1 33intergenic (‑598/‑62)leuL/leuOleu operon leader peptide/DNA‑binding transcriptional dual regulator LeuO
Reads supporting (aligned to +/- strand):  ref base C (1/1);  new base G (21/10);  total (22/11)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.82e-01

AAATAAGCACATTTAATCCATTTTGTAGATGATTGAGTATTCGCGGTAGTTATGATTAGATTGTTTTCGCAACAAAAACATTATGGATTATTATGCTGTGGTAAATGACTCATTCCACGGCAATGGATTCTGTTTTTATCAGAACCCGTATCTTTATGTTTTCCGAATTTTACTCATTTTGCTTTTTCTTATTTTATATGCATGATAAATCATATTCTTCAGGATTATTTCTCTGCATTCCAATAAGGGAAAGGGAGTTAAGTGTGACAGTGGAGTTAAGTATGCCAGAGGTACAAACAGATCATCCAGAGACGGCGGAGTTAAGCAAACCACAGCTACGCATGGTCGATCTCAACTTATTAACCGTTTTCGATGCCGTGATGCAGGAGCAAAACATTACTCGTGCCGCTCATGTTCTGGGAATGTCGCAACCTGCGGTCAGTAACGCT  >  NC_000913/84087‑84535
                                                                                                                                                                                                                          ||                                                                                                                                                                                                                                     
aaaTAAGCACATTTAAGTCATTGTGTAGATGATTGAGTATTCGCGGTAGTTATGATTATATTGTTTTCGCAACACAAACATTATGTATTATTATGCTTTGGTAAATGACTCATTCCACGGTAATGGATTCTGTTTTTATCAGAACCCGTATCTTTATGTTTTCCTAATTTTACGCATGTTCCTTTGTCTTATTTTATATGCATGATAAATCATATTCTCGAGGATTATTACTCTGCATTCCAATaagtgat                                                                                                                                                                                                        <  2:435853/251‑5 (MQ=255)
 aaTAAGCACATTTAATCCATTTTGTAGATGATTGAGTATTCGCGGTAGTTATGATTAGATTGTTTTCGCAACAAAAACATTATGGATTATTATGCTGTGGTAAATGACTCATTCCACGGCAATGGATTCTGTTTTTATCAGAACCCGTATCTTTATGTTTTCCTAATTTTACTCATTTTCCTTTTTCTTATTTTATATGCATGATAAATCATATTCTCGAGGATTATTTCTCTGCATTCCAATaagggaa                                                                                                                                                                                                        >  1:275104/1‑250 (MQ=255)
 aaTAAGCAAATTTACTCAATTTTGTAGGTGATTGAGTATTCGCGGAAGTTATGATTAGATTGTTTTCGCAACAAAAACATTATGGATTATTATGCTGTGGTCAATGGCTCATTCCACGACAATGACTTCTGTTTTTATCAGAACCCGGAGCTTTATATTTTCCTAATTTTACTCATTTTACGTTTTCATATTTTAGATGCATAATAAATCTTATACTCGAGGATTATTTCTATGCATTCCACTaagggaa                                                                                                                                                                                                        >  2:136496/1‑250 (MQ=255)
                     tttGTAGATGATTGAGTATTCGCGGTAGTTATGATTAGATTGTTTTCGCAACAAAAACATTATGGATTATTATGCTGTGGTAAATGACTCATTCCACGGCAATGGATTCTGTTTTTATCAGAACCCGTATCTTTATGTTTTCCTAATTTTACTCATTTTCCTTTTTCTTATTTTATATGCATGATAAATCATATTCTCGAGTATTATTTCTCTGCATTCCATTAAGGGAAAGGGTGTTAAGTGTGACAGTg                                                                                                                                                                                   >  2:18207/1‑251 (MQ=255)
                         tAGATGATTGAGTATTAGCGGTAGTTATGATTAGATTGTTTTCGCAACAAAAACATTATGGATTATTATGCTGTGGTAAATGACTCATTCCACGGAAATGGATTCTGTTTTTATCAGAACCCGTATCTTTATGTTTTCCTAATTTTACTCATTTTCCTTTTTCTTATTTTATATGCATGATAAATCATATTCTCGAGGATTATTTCTCTGCATTCCAATAAGGGAAAGGGAGTTAAGTGTGACAGTGGAGt                                                                                                                                                                               >  2:182726/1‑251 (MQ=255)
                                                                                     gATTATTATGCTGTGGTAAATGACTCATTCCACGGCAATGGATTCTGTTTTTATCAGAACCCGTATCTT‑ATGTTTTCCGAATTTTACTCATTTTGCTTTTTCTTATTTTATATGCATGATTAATCATATTCTTCAGGATTATTTCTCTGCATTCCAATAAGGGAAAGGGAGTTAAGTGTGACAGTGGAGTTAAGTATGCCAGAGGTACAAACAGATCATCCAGAGACGGCGGAGTTAAGCAAACCACAGCt                                                                                                                  >  1:89813/1‑251 (MQ=255)
                                                                                         attaaGCTGTTGTAAATGACTCATTCCACGGCAATTGATTCTGTTTTTATCAGAACCCGTATCTTTATGTTTTCCTAATTTTACTCATTTTCCTTTTTCTTATTTTATATGCATGATAAACCATATTCTCGAGGATTATTTCTCTGCATTCCAATAAGGGAAAGGGAGTTAAGTGTGACAGTGGAGTTAAGTATGCCAGAGGTACAAACAGATCATCCAGAGACGGCGGAGTTAAGCAAGCCACAGCTAc                                                                                                                <  2:172288/250‑1 (MQ=255)
                                                                                           tatGCTGTGGTAAATGACTCATTCCACGGCAATGGATTCTGTTTTTATCAGAAACCGTATCTTTATGTTTTCCTAATTTTACTCATTTTCCTTTTTCTTATTTTATATGCATGATAAATCATATTCTCGAGGATTATTTCTCTGCATTCCAATAAGGGAAAGGGAGTTAAGTGTGACAGTGGAGTTAAGTATGCCAGAGGTACAAACAGATCATCCAGAGACGGCGGAGTTAAGCAAGCCACAGCTACGc                                                                                                              >  1:240277/1‑250 (MQ=255)
                                                                                            atGCTGTGGTAAATGACTCATTCCACGGCAATGGATTCTGTTTTTATCAGAACCCGTATCTTTATGTTTTCCTAATTTTACTCATTTTCCTTTTTCTTATTTTATATGCATGATAAATCATATTCTCGAGGATTATTTCTCTGCATTCCAATaagggaa                                                                                                                                                                                                        <  1:329010/159‑1 (MQ=255)
                                                                                            atGCTGTGGTAAATGACTCATTCCACGGCAATGGATTCTGTTTTTATCAGAACCCGTATCTTTATGTTTTCCTAATTTTACTCATTTTCCTTTTTCTTATTTTATATGCATGATAAATCATATTCTCGAGGATTATTTCTCTGCATTCCAATaagggaa                                                                                                                                                                                                        >  2:329010/1‑159 (MQ=255)
                                                                                              gCTGTGGTAAATGACTCATTCCACGGCAATGGATTCTGTTTTTATCAGAACCCGTATCTTTATGTTTTCCTAATTTTACTCATTTTCCTTTTTCTTATTTTATATGCATGATAAATCATATTCTCGAGGATTATTTCTCTGCATTCCAATAAGGGAAAGGGAGTTAAGTGTGACAGTGGAGTTAAGTATGCCAGAGGTACAAACAGATCATCCAGAGACGGCGGAGTTAAGCAAGCCACAGCTACGCATgg                                                                                                          >  1:115746/1‑251 (MQ=255)
                                                                                                            cTCATTCCACGGCAATGGATTCTGTTTTTATCAGAACCCGTATCTTTATGTTTTCCTAATTTTACTCATTTTCCTTTTTCTTATTTTATATGCATGATAAATCATATTCTCGAGGATTATTTCTCTGCATTCCAATAAGGGAAAGGGAGTTAAGTGTGACAGTGGAGTTAAGTATGCCAGAGGTACAAACAGATCATCCAGAGACGGCGGAGTTAAGCAAGCCACAGCTACGCATGGTCGATCTCAACtt                                                                                             >  1:266369/1‑250 (MQ=255)
                                                                                                                 tCCACGGCAATGGATTCTGTTTTTATCAGAACCCGTATCTTTATGTTTTCCTAATTTTACTCATTTTCCTTTTTCTTATTTTATATGCATGATAAATCATATTCTCGAGGATTATTTCTCTGCATTCCAATAAGGGAAAGGGAGTTAAGTGTGACAGTGGAGTTAAGTATGCCAGAGGTACAAACAGATCATCCAGAGAcgg                                                                                                                                        >  2:333649/1‑202 (MQ=255)
                                                                                                                 tCCACGGCAATGGATTCTGTTTTTATCAGAACCCGTATCTTTATGTTTTCCTAATTTTACTCATTTTCCTTTTTCTTATTTTATATGCATGATAAATCATATTCTCGAGGATTATTTCTCTGCATTCCAATAAGGGAAAGGGAGTTAAGTGTGACAGTGGAGTTAAGTATGCCAGAGGTACAAACAGATCATCCAGAGAcgg                                                                                                                                        <  1:333649/202‑1 (MQ=255)
                                                                                                                                ttggTTTTTACCAGAACCCGGTTCTTTATTTTTTCCTAATTTTCCTCATTTTCCTTTTTCTTATTTTATATGCATGATAAATCATATTCGCGAGGATTATTTCTCTGCATTCCAATAAGGGAAAGGGAGTTAAGTGTGACAGTGGAGTTAAGTATGCCAGAGTTACAAACAGATCATCCAGAGACGGCGGAGTTAAGCAAGCCACAGCTACGCATGGTCGATCTCAACTTATTAACCGTTTTCGATgtcg                                                                         <  2:249260/247‑4 (MQ=255)
                                                                                                                                   gTTTTTATCAGAACCCGTATCTTTATGTTTTCCTAATTTTACTCATTTTCCTTTTTCTTATTTTATATGCATGATAAATCATATTCTCGAGGATTATTTCTCTGCATTCCAATAAGGGAAAGGGAGTTAAGTGTGACAGTGGAGTTAAGTATGCCATAGGTACAAACAGATCATCCAGAGACGGCGTAGTTAAGCAAGCCACAGCTACGCATGGTCGATCTCAACTTATTAACCGTTTTCGATGCCGTGAt                                                                     <  2:145684/251‑1 (MQ=255)
                                                                                                                                                     aTCTTTATGTTTTCCTAATTTTACTCATTTTCCTTTTTCTTATTTTATATGCATGATAAATAATATTCTCGAGGTTTATTTCTCTGCATTCCAATATGGGAAAGGAAGTTAAGTGTGACAGTGGAGTTAAGTATGCAAGAGGTACAAACAGATCATCCAGAGCCGGCGGAGTTAAGCAAGCCACAACTACGCATGGTCAACCTCAACTTATTAACCGTTTTCGATGCCGTGATGCAGGGGCAAAACATTat                                                   >  2:25029/1‑250 (MQ=255)
                                                                                                                                                            tGTTTTCCTAATTTTACTCATTTTCCTTTTTTTTATTTTATATGCATGATAAATCATATTCTCGAGGATTATTTCTCTGCATTCCAATAAGGGAAAGGGAGTTAAGTGTGACAGGGGAGTTAAGTATGCCAGAGGTACAAACAGATCATCCAGAGACGGCGGAGTTAAGCAAGCCACAGCTACGCATGGTCGATCTCAACTTATTAACCGt                                                                                    <  2:354066/211‑1 (MQ=255)
                                                                                                                                                            tGTTTTCCTAATTTTACTCATTTTCCTTTTTCTTATTTTATATGCATGATAAATCATATTCTCGAGGATTATTTCTCTGCATTCCAATAAGGGAAAGGGAGTTAAGTGTGACAGTGGAGTTAAGTATGCCAGAGGTACAAACAGATCATCCAGAGACGGCGGAGTTAAGCAAGCCACAGCTACGCATGGTCGATCTCAACTTATTAACCGt                                                                                    >  1:354066/1‑211 (MQ=255)
                                                                                                                                                             gTTTTCCTAATTTTACTCCTTTTCCTTTTTCTTATTTTATATGCTTGATAAATCATCTTCTCGAGGATTCTTTCTCTGCATTCCAATAAGGGAAAGGGAGTTAAGTGTGACAGTGGAGTTAAGTATGCCAGAGGTACAAACAGATCATCCAGCGACGGCGGCGTTAAGCAAGCCACCGCTACGCATGGTCGATCTCAACTTCTTAACCGTTTTCGATGCCGTGATGCAGGGGCAAAACATTACTCGTGCCg                                           >  2:438287/1‑251 (MQ=255)
                                                                                                                                                                    taaTTTTACTCATTTTCCTTTTTCTTATTTTATATGCATGATAACTCATATTCTCGAGGATTATTTCTCTGCATTCCAATAAGGGAAAGGGAGTTAAGTGTGACAGTGGAGTTAAGTATGCCAGAGGAACAAACAGATCATCCAGAGACGGCGGAGTTAAGCAAGCCACAGCTACGCATGGTCGATCTCAACTTATTAACCGTTTTCGATGCCGTGTTGCAGGAGCAAAACATTACTCTTGCCGCTCATGt                                    >  1:289902/2‑251 (MQ=255)
                                                                                                                                                                             tCATTTTCCTTTTTCTTATTTTATATGCATGATAAATCATATTCTCGAGGATTATTTCTCTGCATTCCAATAAGGGAAAGGGAGTTAAGTGTGACAGTGGAGTTAAGTATGCCAGAGGTACAAACAGATCATCCAGAGACGGCGGAGTTAAGCAAGCCACAGCTACGCATGGTCGATCTCAACTTATTAACCGTTTTCGATGCCGTGATGCAGGAGCAAAACATTACTCGTGCCGCTCATGTTCTGGGAAt                           >  1:77546/1‑251 (MQ=255)
                                                                                                                                                                                 tttccTTTTTCTTATTTTATATGCATGATAAATCATATTCTCGGGGATTATTTCTCTGCATTCCAATATGGGAAAGGGAGTTAAGTGTGACAGTGGAGTTAAGTATGCCAGAGGTACAAACAGATCATCCAGAGACGGCGGAGTTAAGCAAGCCACAGCTACGCATGGTCGATCTCAACTTATTAACCGTTTTCGATGCCGTGATGCAGGAGCAACACCTTACGCGTGCCGCTCATGTTCTGGGAATGTCg                       >  2:323639/5‑251 (MQ=255)
                                                                                                                                                                                        tttCTTATTGTAAATGCATAATAAATCATATTATCGAGGATTATTTCGTTGAATTCCAATAAGGGAAAGGGAGTTAAGGGTGACAGTGGAGTTAAGTATGCCAGAGGTACAAACAGTTCACCCAGAGACGGCGGATTTAAGCAAGCCACAGCTACGCATGGTCGATCTCAACTTATTAACCGTTTTCGATGCCGTGATGCAGGAGCAAAACATTACTCGTGCCGCTCATGTTCTGGGAATGTCGCAACCt                 <  2:168378/250‑1 (MQ=255)
                                                                                                                                                                                            ttATTTTATATGCATGATAAATCATATTCTCGAGGATTATTTCTCTGCATTCCAATAAGGGAAAGGGAGTTAAGTGTGACAGTGGAGTTAAGTATGCCAGAGGTACAAACAGATCATCCAGAGACGGCGGAGTTAAGCAAGCCACAGCTACGCATGGTCGATCTCAACTTATTAACCGTTTTCGATGCCGTg                                                                       >  2:124826/1‑192 (MQ=255)
                                                                                                                                                                                            ttATTTTATATGCATGATAAATCATATTCTCGAGGATTATTTCTCTGCATTCCAATAAGGGAAAGGGAGTTAAGTGTGACAGTGGAGTTAAGTATGCCAGAGGTACAAACAGATCATCCAGAGACGGCGGAGTTAAGCAAGCCACAGCTACGCATGGTCGATCTCAACTTATTAACCGTTTTCGATGCCGTg                                                                       <  1:124826/192‑1 (MQ=255)
                                                                                                                                                                                              aTTTTATATGCATGATTAATCATATTCTTCAGGATTATTTCTCTGCATTCCAATAAGGGAAAGGGAGTTAAGTGTGACAGTGGAGTTAAGTATGCCAGAGGTACAAACAGATCATCCAGAGACGGCGGAGTTAAGCAAACCACAGCTACGCATGGTCGATCTCAACTTATTAACCGTTTTCGATGCCGTGATGCAGGAGCAAAACATTACTCGTGCCGCTCATGTTCTGGGAATGTCGCAACCTGCGGTc           <  2:89813/250‑1 (MQ=255)
                                                                                                                                                                                              aTTTTATATGCATGATAAATCATATTCTCGAGGATTATTTCTCTGCATTCCAATAAGGGAAAGGGAGTTAAGTGTGAAAGTGGAGTTAAGTATGCCAGAGGTACAAACAGATCATCCAGAGACGGCGGAGTTAAGCAAGCCACAGCTACGCATGGTCGATCTCAACTTATTAACCGTTTTCGATGCCGTGATGCAGGAGCAAAACATTACTCGTGCCGCTCATGTTCTGGGAATGTCGCAACCTGCGGTc           >  1:190446/1‑250 (MQ=255)
                                                                                                                                                                                                  tataTGCATGATAAATCATATTCTCGAGGATTATTTCTCTGCATTCCAATAAGGGAAAGGGAGTTAAGTGTGACAGTGGAGTTAAGTATGCCAGAGGTACAAACAGATCATCCAGAGACGGCGGAGTTAAGCAAGCCACAGCTACGCATGGTCGATCTCAACTTATTAACCGTTTTCGATGCCGTGATGCAGGAGCAAAACATTACTCGTGCCGCTCATGTTCTGGGAATGTCGCAACCTGCGGTCAGTa       >  1:101256/1‑250 (MQ=255)
                                                                                                                                                                                                    tatGCATGATAAATCATATTCTCGAGGATTATTTCTCTGCATTCCAATAAGGGAAAGGGAGTTAAGTGTGACAGTGGAGTTAAGTATGCCAGAGGTACAAACAGATCATCCAGAGACGGCGGAGTTAAGCAAGCCACAGCTACGCATGGTCGATCTCAACTTATTAACCGTTTTCGATGCCGTGATGCAGGAGCAAAACATTACTCGTGCCGCTCCTGTTCTGGGAATGTCGCAACCTGCGGTCAGTAACg    >  1:211690/1‑251 (MQ=255)
                                                                                                                                                                                                     atGCATGATAAATCATATTCTCGAGGATTATTTCTCTGCCTTCCAATATGGGAAAGGTAGTTAAGTGTGACAGTGGAGTTAAGTATGCCAGAGGTACAAACAGATCATCCAGCGACGGCGGAGTTAAGCAAGCCACAACTACGCATGGTCGATCTCAACTTATTAACCTTTTTCGATGCCGTGATGCAGGAGCAAAACATTACTCGTGCCGACCATGTTCTGGGAATGTCGCAACCAGAGGTCAGTAACGc   >  2:295197/1‑251 (MQ=255)
                                                                                                                                                                                                     atGCATGATAAATCATATTCTCGAGGATTATTTCTCTGCATTCCAATAAGGGAAAGGGAGTTAAGTGTGACAGTGGAGTTAAGTATGCCAGAGGTACAAACAGATCATCCAGAGACGGCGGAGTTAAGCAAGCCACAGCTACGCATGGTCGATCTCAACTTATTAACCGTTTTCGATGCCGTGATGCAGGAGCAAAACATTACTCGTGCCGCTCATGTTCTGGGAATGTCGCAACCTGCGGTCAGTAACg    <  1:143743/250‑1 (MQ=255)
                                                                                                                                                                                                      tGCATGATAAATCATATTCTCGAGGATTATTTCTCTGCATTCCAATAAGGGAAAGGGAGTTAAGTGTGACAGTGGAGTTAAGTATGCCAGAGGTACAAACAGATCATCCAGAGACGGCGGAGTTAAGCAAGCCACAGCTACGCATGGTCGATCTCAACTTATTAACCGTTTTCGATGCCGTGATGCAGGAGCAAAACATTACTCGTGCCGCTCATGTTCTGGGAATGTCGCAACCTGCGGTCAGTAACGCt  >  1:43627/1‑251 (MQ=255)
                                                                                                                                                                                                                          ||                                                                                                                                                                                                                                     
AAATAAGCACATTTAATCCATTTTGTAGATGATTGAGTATTCGCGGTAGTTATGATTAGATTGTTTTCGCAACAAAAACATTATGGATTATTATGCTGTGGTAAATGACTCATTCCACGGCAATGGATTCTGTTTTTATCAGAACCCGTATCTTTATGTTTTCCGAATTTTACTCATTTTGCTTTTTCTTATTTTATATGCATGATAAATCATATTCTTCAGGATTATTTCTCTGCATTCCAATAAGGGAAAGGGAGTTAAGTGTGACAGTGGAGTTAAGTATGCCAGAGGTACAAACAGATCATCCAGAGACGGCGGAGTTAAGCAAACCACAGCTACGCATGGTCGATCTCAACTTATTAACCGTTTTCGATGCCGTGATGCAGGAGCAAAACATTACTCGTGCCGCTCATGTTCTGGGAATGTCGCAACCTGCGGTCAGTAACGCT  >  NC_000913/84087‑84535

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑