Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,088,010 2 bp→TT intergenic (+150/‑273) metK → / → galP methionine adenosyltransferase/galactose:H(+) symporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,088,0100CT82.8% 59.5 / 8.0 29intergenic (+150/‑274)metK/galPmethionine adenosyltransferase/galactose:H(+) symporter
Reads supporting (aligned to +/- strand):  ref base C (3/2);  new base T (7/17);  total (10/19)
Fisher's exact test for biased strand distribution p-value = 3.06e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.76e-01
*NC_0009133,088,0110CT82.8% 57.8 / 8.0 29intergenic (+151/‑273)metK/galPmethionine adenosyltransferase/galactose:H(+) symporter
Reads supporting (aligned to +/- strand):  ref base C (3/2);  new base T (7/17);  total (10/19)
Fisher's exact test for biased strand distribution p-value = 3.06e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.12e-01

ACCGCAGCATACGGTCACTTTGGTCGTGAACATTTCCCGTGGGAAAAAACCGACAAAGCGCAGCTGCTGCGCGATGCTGCCGGTCTGAAGTAATCTTTCTTCACCTGCGTTCAAAGGCCAGCCTCGCGCTGGCCTTTTTCTTTTGGATAGGCGTTCACGCCGCATCCGGCAAAAAAACCGCCCGCACAATAACATCATTCTTCCTGATCACGTTTCACCGCAGATTATCATCACAACTGAAACCGATTACACCAACCACAACAGACAAAGATTTGTAATATTTTCATATTATTATTCGGTTTTCACAGTTGTTACATTTCTTTTCAGTAAAGTCTTAATTGCAGATAACAGCGTTTAATCTATGATGATATAACTCAATTATTTTCATGCACTTAAATCATAACTAAGATAAATGTTAGTGTAAGCGATTACACTGATGTGATTTGCTTCACATCTTTTTACGTCGTACTCACCTATCTTAATTCA  >  NC_000913/3087768‑3088253
                                                                                                                                                                                                                                                  ||                                                                                                                                                                                                                                                  
aCCGCAGCATACGGTCACTTTGGTCGTGAACATTTCCCGTGGGAAAAAACCGACAAAGCGCAGCTGCTGCGCGATGCTGCCGGTCTGAAGTAATCTTTCTTCACCTGCGTTCAAAGGCCAGCCTCGCGCTGGCCTTTTTCTTTTTGATAGGCGTTCACGCCGCATCCGGCAAAAAAACCGCCCGCACAATAACATCATTCTTCCTGATCACGTTTCACCGCAGATTATCATCACAACTGAAATTGATTac                                                                                                                                                                                                                                              >  1:274910/1‑250 (MQ=255)
                       tCGTGAACATTTCCCGTGGGAAAAAACCGACAAAGCGCAGCTGCTGCGCGATGCTGCCGGTCTGAAGTAATCTTTCTTCACCTGCGTTCAAAGGCCAGCCTCGCGCTGGCCTTTTTCTTTTGGATAGGCGTTCACGCCGCATCCGGCAAAAAAACCGCCCGCACAATAACATCATTCTTCCTGATCACGTTTCACCGCAGATTATCATCACAACTGAAATTGATTACACCAACCACAACAGACAAAGAttt                                                                                                                                                                                                                      >  1:19060/1‑251 (MQ=255)
                          tGAACATTTCCCGTGGGAAAAAACCGACAAAGCGCAGCTGCTGCGCGATGCTGCCGGTCTGAAGTAATCTTTCTTCACCTGCGTTCAAAGGCCAGCCTCGCGCTGGCCTTTTTCTTTTGGATAGGCGTTCACGCCGCATCCGGCAAAAAAACCGCCCGCACAATAACATCATTCTTCCTGATCACGTTTCACCGCAGATTATCATCACAACTGAAATTGATTACACCAACCACAACAGACAAAGAttt                                                                                                                                                                                                                      <  1:374966/248‑1 (MQ=255)
                             aatttccccggggaaaaaaaCCGCCAAAGCGCATCTTCTGCGCGATGCTGCCGGTCGGAAGTAATCTTTCTTCACCTGCGTTCAAAGGCCCGCCCCGCGCTGGCCTTTTTCTTTGGGATAGGCGTCCACGCCGCATCCGGCAAAAAAACCGCCCGCACAATAACATCATTCTTCCTGATCACGTTTCACCGCAGATTATCATCACAACTGAAATTGATTAAACCAACCACAACAGACAAAGATTTGTAata                                                                                                                                                                                                                <  2:311068/237‑1 (MQ=255)
                                    ccGTGGGAAAAAACCGACAACGCGCAGCGGCTGCGCGATGCTGCCGGTCTGAAGTAATCTTTCTTCACCTGCGTTCAAAGGCCAGCCTCGCGCTGGCCTTTTTCTTTTGGATAGGCGTTCACGCCGCATCCGGCAAAAAAACCGCCCGCACAATAACATCATTCTTCCTGATCACGTTTCACCGCAGATTATCATCACAACTTAAATTGATTACACCAACCACAACAGACAAAGATTTGTAATATTTTCat                                                                                                                                                                                                         <  2:93619/251‑1 (MQ=255)
                                          gAAAAAACCGACAAAGCGCAGCTGCTGCGCGATGCTGCCGGTCTGAAGTAATCTTTCTTCACCTGCGGTCAAAGGCCAGCCTCGCGCTGGCCTTTTTCTTTTGGATAGGCGTTCACGCCGCATCCGGCAAAAAAACCGCCCGCACAATAACATCATTCTTCCTGATCACGTTTCACCGCAGATTATCATCACAACTGAAATTGATTACACCAACCACAACAGACAAAGATTTGTAATATGTTCAtattatt                                                                                                                                                                                                   <  2:325275/251‑1 (MQ=255)
                                          gAAAAAACCGACAAACCCCAGCGGCTGGGCGATGCTGCCGGTCTGAATTAATCTTTCTTCACCTGCGTTCAAAGGCCAGCCTCGCGCTGGCCTTTTTCTTTTGGATAGGCGTTCACGCCGCATCCGGCAAAAAAACCGCCCGCACAATAACATCATTCTTCCTGATCACGTTTCACCGCAGATTATCATCACAACTGAAATTGATTACACCAACCACAACAGACAAAGATTTGTAATATTTTCAtattatt                                                                                                                                                                                                   <  2:330794/251‑1 (MQ=255)
                                                       aaGCGCATCTGCTGCCCGATGCTGCTGGTCTGAAGTAATCTTTCTTCCCCTGCGTTAAAAGGCCAGCCACGCGCTGGCCTTTTCCTTTTGGATAGGCGTTCACGCCGCATCCGGCAAAAAAACCGCCCGCACAATAACATCATTCTTCCTGATCACGTTTCACCGCAGATTATCATCACAACTGAAATTGATTACACCAACCACAACAGACAAAGATTTGTAATATTTTCATATTATTATTCGGTTTTcac                                                                                                                                                                                      <  2:141886/251‑1 (MQ=255)
                                                        agcgcagtcgttgcacgATGCTGACGTGCTGAAGTAATCTTTCTTCACCTGCGTTCAAATGCCAGCCTCGCGCTGGCCTTTTTCTTTTGGATAGGCGTTCACGCCGCATCCGGCAAAAAAACCGCCCGCACAATAACATCATTCTTCCTGATCACGTGTCACCGCAAATTATCATCACAACTGATATTGATTACACCAACCACAACAGACAAAGATTTGTAATATTTTCATATTATTATTCGGGTTTcaca                                                                                                                                                                                     <  2:360619/236‑1 (MQ=255)
                                                         gcgaaGCTGCTGCTCGATGCTGCCGGTCTGAAGTAATCTTTCTTCACCTGCGTTCAAAGGCCAGCCTCGCGCTGGCCTTTTTCTTTTGGATAGGCGTTCACGCCGCATCCGGCAAAAAAACCGCCCGCACAATAACATCATTCTTCCTGATCACGTTTCACCGCAGATTATCATCACAACTGAAATTGATTACACCAACCACAACAGACAAAGATTTGTAATATTTTCATATTATTATTCGGTTTTCACAg                                                                                                                                                                                    <  1:366881/247‑1 (MQ=255)
                                                              gctgctGCGCGATGCTGCCGGTCTGAAGTAATCTTTCTTCCCCTGCGTTCAAAGGCCAGCCTCGCGCTGGCCTTTTTCTTTTGGATAGGCGTTCACGCCGCATCCGGCAAAAAAACCGCCCGCACAATAACATCATTCTTCCTGATCACGTTTCACCGCAGATTATCATCACAACTGAAATTGATTACACCAACCACAACAGACAAAGATTTGTAATATTTTCATATTATTATTCGGTTTTCACAgttgtt                                                                                                                                                                               <  1:162162/251‑1 (MQ=255)
                                                              ccgggtgtggatttctccagggcTGAAGTAAGCTTTCTTGCGCTGCGTGCAAAGGCCCGCCCCGCGCTTTACTTTTTCTGTTGGATAGGCGTTCCCGCGGCATCCGGCAAAAAAACCGCACGCACAATAACATCATTCTTTCTGATCACGTTTCACCGCAGATTATCATCACATCTGAAATTGATTACACCAACCACAACAGACAAAGATTTGTAATAATTTCATATTAGTATTCGGTTTTCATAgtggtt                                                                                                                                                                               <  2:246026/229‑1 (MQ=255)
                                                                     cgcgATGCTGCCGGTCTGAAGTAATCTTTCTTCACCTGCGTTCAAAGGCCAGCCTCGCGCTGGCCTTTTTCTTTTGGATAGGCGTTCACGCCGCATCCGGCAAAAAAACCGCCCGCACAATAACATCATTCTTCCTGATCACGTTTCACCGCAGATTATCATCACAACTGAAACCGATTACACCAACCACAACAGACAAAGATTTGTAATATTTTCATATTATTATTCGGTTTTCACAGTTGTTACATTTc                                                                                                                                                                        >  1:231401/1‑251 (MQ=255)
                                                                                                             ttCAAAGGCCAGCCTCGCGCTGGCCTTTTTCTTTTGGCTAGGCGTTCACGCCGCATCCGGCAAAAAAACCGCCCGCACAATAACATCATTCTTCCTGATCACGTTTCACCGCAGATTATCATCACAACTGAAATTGATTACACCAACCACAACAGACAAAGATTTGTAATATTTTCATATTATTATTCGGTTTTCACAGTTGTTACATTTCTTTTCAGTAAAGTCTTAATTGCAGATAACAGCGTTTAATc                                                                                                                                >  1:6148/1‑251 (MQ=255)
                                                                                                                 aaGGCCAGCCTCGCGCTGGCCTTTTTCTTTTGGATAGGCGTTCACGCCGCCTCCGGCAAAAACACCGCCCGCACAATAACATCATTCTTCCTGATCACGTTTCACCGCAGATTATCATCACAACTGAAACCGATTACACCAACCACAACAGACAAAGATTTGTAATATTTTCATATTATTATTCGGTTTTCACAGTTGTTACATTTCTTTTCAGTAAATTCTTAATTGCAGATAACAGCGTTTAATCTatg                                                                                                                            >  2:120324/1‑251 (MQ=255)
                                                                                                                 aaGGCCAGCCTCGCGCTGGCCTTTTTCTTTTGGATAGGCGTTCACGCCGCATCCGGCAAAAAAACCGCCCGCACAATAACATCATTCTTCCTGATCACGTTTCACCGCAGATTATCATCACAACTGAAATTGATTACACCAACCACAACAGACAAAGATTTGTAATATTTTCATATTATTATTCGGTTTTCACAGTTGTTACATTTCTTTTCAGTAAAGTCTTAATTGCAGATAACAGCGTTTAATCTatg                                                                                                                            <  1:237774/251‑1 (MQ=255)
                                                                                                                        accTCGCGCGGGCATTTTTCTTTTGGATAGGCGTTCACGCCGCATCCGGCAAAAAAACCGCCCGCACAATAACATCATTCTTCCTGATCACGTTTCACCGCAGATTATCATCACAACTGAAATTGATTACACCAACCACAACAGACAAAGATTTGTAATATTTTCATATTATTATTCGGTTTTCACAGTTGTTACATTTCTTTTCAGTAAAGTCTTAATTGCAGATAACAGCGTTTAATCTATGATGatat                                                                                                                     <  2:406744/250‑1 (MQ=255)
                                                                                                                                  ggCCTTTTTCTTTTGGATAGGCGTTCACGCCGCATCCGGCAAAAAAACCGCCCGCACAATAACATCATTCTTCCTGATCACGTTTCACCGCAGATTATCATCACAACTGAAATTGATTACACCAACCACAACAGACAAAGATTTGTAATATTTTCATATTATTATTCGGTTTTCACAGTTGTTACATTTCTTTTCAGTAAAGTCTTAATTGCAGATAACAGCGTTTAATCTATGATGATATAACTCAatt                                                                                                            <  1:219076/250‑1 (MQ=255)
                                                                                                                                          tCTTTTGGATAGGCGTTCACGCCGCATCCGGCAAAAAAACCGCCCGCACAATAACATCATTCTTCCTGATCACGTTTCACCGCAGATTATCATCACAACTGAAATTGATTACACCAACCACAACAGACAAAGATTTGTAATATTTTCATATTATTATTCGGTTTTCACAGTTGTTACATTTCTTTTCAGTAAAGTCTTAATTGCAGATAACAGCGTTTAATCTATGATGATATAACTCAATTATTTTCATg                                                                                                   >  1:328661/1‑251 (MQ=255)
                                                                                                                                                               ccgcATCCGGCAAAAAAACCGCCCGCACAATAACATCATTCTTCCTGATCACGTTTCACCGCAGATTATCATCACAACTGAAATTGATTACACCAACCACAACAGACAAAGATTTGTAATATTTTCATATTATTATTCGGTTTTCACAGTTGTTACATTTCTTTTCAGTAAAGTCTTAATTGCAGATAACAGCGTTTAATCTATGATGATATAACTCAATTATTTTCATGCACTTAAATCATAACTAAGAt                                                                              <  1:340506/251‑1 (MQ=255)
                                                                                                                                                                                                   catTCTTCCTGATCACGTTTCACCGCAGATTATCATCACAACTGAAATTGATTACACCAACCACAACAGACAAAGATTTGTAATATTTTCATATTATTATTCGGTTTTCACAGTTGTTACATTTCTTTTCAGTAAAGTCTTAATTGCAGATAACAGCGTTTAATCTATGATGATATAACTCAATTATTTTCATGCACTTAAATCATAACTAAGATAAATGTTAGTGTAAGCGATTACACTGATGTGATTTg                                          >  1:244811/1‑251 (MQ=255)
                                                                                                                                                                                                             gATCACGTTTCACCGCAGATTATCATCACAACTGAAATTGATTACACCAACCACAACAGACAAAGAGTTGTAATATTTTCATATTATTATTCGGTTTTCACAGTTGTTACATTTCTTTTCAGTAAAGTCTTAATTGCAGATAACAGCGTTTAATCTATGATGATATAACTCAATTATTTTCATGCACTTAAATCATAACTAAGATAAATGTTAGTGTAAGCGATTACACTGATGTGATTTGCTTCACAt                                  <  2:328661/249‑1 (MQ=255)
                                                                                                                                                                                                                    tttCACCGCAGATTATCATCACAACTGAAATTTATTACACCAACCACAACAGACAAAGATTTGTAATATTTTCATCTTATTATTCGGTTTTCACAGTTGTTACATTTCTTTTCAGTAAAGTCTTAATTACAGATAACAGCGATTAATCTATGATGATATAACTCAATTATTTTCATGCACTTAAATCATAACTAAGATAACTGTTAGTGTCAGCCATACCACTGATGTGATTTTATTCACCTCTTTTTAcg                         >  1:437180/1‑251 (MQ=255)
                                                                                                                                                                                                                     ttCACCGCAGATTATCATCACAACTGAAACCGATTACACCAACCACAACAGACAAAGATTTGTAATATTTTCATATTATTATTCGGTTTTCACAGTTGTTACATTTCTTTTCAGTAAAGTCTTAATTGCAGATAACAGCGtt                                                                                                                                     >  2:259322/1‑142 (MQ=255)
                                                                                                                                                                                                                     ttCACCGCAGATTATCATCACAACTGAAACCGATTACACCAACCACAACAGACAAAGATTTGTAATATTTTCATATTATTATTCGGTTTTCACAGTTGTTACATTTCTTTTCAGTAAAGTCTTAATTGCAGATAACAGCGtt                                                                                                                                     <  1:259322/142‑1 (MQ=255)
                                                                                                                                                                                                                       cACCGCAGATTATCATCACAACTGAAATTGATTACACCAACCACAACAGACAAAGATTTGTAATATTTTCATATTATTATTCGGTTTTCACAGTTGTTACATTTCTTTTCAGTAAAGTCTTAATTGCAGATAACAGCGTTTAATCTATGATGATATAACTCAATTATTTTCATGCACTTAAATCATAACTAAGATAAATGTTAGTGTAAGCGATTACACTGATGTGATTTGCTTCACATCTTTTTAcgtcg                      >  2:210906/1‑251 (MQ=255)
                                                                                                                                                                                                                          cGCAGATTATCATCACAACTGAAACCGATTACACCAACCACAACAGACAAAGATTTGTAATATTTTCATATTATTATTCGGTTTTCACAGTTGTTACATTTCTTTTCAGTAAAGTCTTAATTGCAGATAACAGCGTTTAATCTATGATGATATAACTCAATTATTTTCATGCACTTAAATCATAACTAAGATAAATGTTAGTGTAAGCGATTACACTGATGTGATTTGCTTCACATCTTTTTACGTCGTAc                   <  1:120324/251‑1 (MQ=255)
                                                                                                                                                                                                                                  atcatcACAACTGAAATTGATTACACCAACCACAACAGACAAAGATTTGTAATATTTTCATATTATTATTCGGTTTTCACAGTTGTTACATTTCTTTTCAGTAAAGTCTTAATTGCAGATAACAGCGTTTAATCTATGATGATATAACTCAATTATTTTCATGCACTTAAATCATAACTAAGATAAATGTTAGTGTAAGCGATTACACTGATGTGATTTGCTTCACATCTTTTTACGTCGTACTCACCTAt           <  1:210906/251‑1 (MQ=255)
                                                                                                                                                                                                                                           aCTGAAATTGATTACACCAACCACAACAGACAAAGATTTGTAATATTTTCATATTATTATTCGGTTTTCACAGTTGTTACATTTCTTTTCAGTAAAGTCTTAATTGCAGATAACAGCGTTTAATCTATGATGATATAACTCAATTATTTTCATGCACTTAAATCATAACTAAGATAAATGTTAGTGTAAGCGATTACACTGATGTGATTTGCTTCACATCTTTTTACGTCGTACTCACCTATCTTAATTca  <  1:257701/251‑1 (MQ=255)
                                                                                                                                                                                                                                                  ||                                                                                                                                                                                                                                                  
ACCGCAGCATACGGTCACTTTGGTCGTGAACATTTCCCGTGGGAAAAAACCGACAAAGCGCAGCTGCTGCGCGATGCTGCCGGTCTGAAGTAATCTTTCTTCACCTGCGTTCAAAGGCCAGCCTCGCGCTGGCCTTTTTCTTTTGGATAGGCGTTCACGCCGCATCCGGCAAAAAAACCGCCCGCACAATAACATCATTCTTCCTGATCACGTTTCACCGCAGATTATCATCACAACTGAAACCGATTACACCAACCACAACAGACAAAGATTTGTAATATTTTCATATTATTATTCGGTTTTCACAGTTGTTACATTTCTTTTCAGTAAAGTCTTAATTGCAGATAACAGCGTTTAATCTATGATGATATAACTCAATTATTTTCATGCACTTAAATCATAACTAAGATAAATGTTAGTGTAAGCGATTACACTGATGTGATTTGCTTCACATCTTTTTACGTCGTACTCACCTATCTTAATTCA  >  NC_000913/3087768‑3088253

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑