Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 84,415 A→G K16K (AAA→AAG)  leuO → DNA‑binding transcriptional dual regulator LeuO

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00091384,4150AG95.0% 118.0 / ‑1.4 40K16K (AAA→AAG) leuODNA‑binding transcriptional dual regulator LeuO
Reads supporting (aligned to +/- strand):  ref base A (1/1);  new base G (26/12);  total (27/13)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.49e-01

GATTATTATGCTGTGGTAAATGACTCATTCCACGGCAATGGATTCTGTTTTTATCAGAACCCGTATCTTTATGTTTTCCGAATTTTACTCATTTTGCTTTTTCTTATTTTATATGCATGATAAATCATATTCTTCAGGATTATTTCTCTGCATTCCAATAAGGGAAAGGGAGTTAAGTGTGACAGTGGAGTTAAGTATGCCAGAGGTACAAACAGATCATCCAGAGACGGCGGAGTTAAGCAAACCACAGCTACGCATGGTCGATCTCAACTTATTAACCGTTTTCGATGCCGTGATGCAGGAGCAAAACATTACTCGTGCCGCTCATGTTCTGGGAATGTCGCAACCTGCGGTCAGTAACGCTGTTGCACGCCTGAAGGTGATGTTTAATGACGAGCTTTTTGTTCGTTATGGCCGTGGTATTCAACCGACTGCTCGCGCATTTCAACTTTTTGGTTCAGTTCGTCAGGCATTGCAACTAGTACAAAAT  >  NC_000913/84172‑84661
                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                      
gATTATTATGCTGTGGTAAATGACTCATTCCACGGCAATGGATTCTGTTTTTATCAGAACCCGTATCTT‑ATGTTTTCCGAATTTTACTCATTTTGCTTTTTCTTATTTTATATGCATGATTAATCATATTCTTCAGGATTATTTCTCTGCATTCCAATAAGGGAAAGGGAGTTAAGTGTGACAGTGGAGTTAAGTATGCCAGAGGTACAAACAGATCATCCAGAGACGGCGGAGTTAAGCAAACCACAGCt                                                                                                                                                                                                                                                >  1:89813/1‑251 (MQ=255)
    attaaGCTGTTGTAAATGACTCATTCCACGGCAATTGATTCTGTTTTTATCAGAACCCGTATCTTTATGTTTTCCTAATTTTACTCATTTTCCTTTTTCTTATTTTATATGCATGATAAACCATATTCTCGAGGATTATTTCTCTGCATTCCAATAAGGGAAAGGGAGTTAAGTGTGACAGTGGAGTTAAGTATGCCAGAGGTACAAACAGATCATCCAGAGACGGCGGAGTTAAGCAAGCCACAGCTAc                                                                                                                                                                                                                                              <  2:172288/250‑1 (MQ=255)
      tatGCTGTGGTAAATGACTCATTCCACGGCAATGGATTCTGTTTTTATCAGAAACCGTATCTTTATGTTTTCCTAATTTTACTCATTTTCCTTTTTCTTATTTTATATGCATGATAAATCATATTCTCGAGGATTATTTCTCTGCATTCCAATAAGGGAAAGGGAGTTAAGTGTGACAGTGGAGTTAAGTATGCCAGAGGTACAAACAGATCATCCAGAGACGGCGGAGTTAAGCAAGCCACAGCTACGc                                                                                                                                                                                                                                            >  1:240277/1‑250 (MQ=255)
         gCTGTGGTAAATGACTCATTCCACGGCAATGGATTCTGTTTTTATCAGAACCCGTATCTTTATGTTTTCCTAATTTTACTCATTTTCCTTTTTCTTATTTTATATGCATGATAAATCATATTCTCGAGGATTATTTCTCTGCATTCCAATAAGGGAAAGGGAGTTAAGTGTGACAGTGGAGTTAAGTATGCCAGAGGTACAAACAGATCATCCAGAGACGGCGGAGTTAAGCAAGCCACAGCTACGCATgg                                                                                                                                                                                                                                        >  1:115746/1‑251 (MQ=255)
                       cTCATTCCACGGCAATGGATTCTGTTTTTATCAGAACCCGTATCTTTATGTTTTCCTAATTTTACTCATTTTCCTTTTTCTTATTTTATATGCATGATAAATCATATTCTCGAGGATTATTTCTCTGCATTCCAATAAGGGAAAGGGAGTTAAGTGTGACAGTGGAGTTAAGTATGCCAGAGGTACAAACAGATCATCCAGAGACGGCGGAGTTAAGCAAGCCACAGCTACGCATGGTCGATCTCAACtt                                                                                                                                                                                                                           >  1:266369/1‑250 (MQ=255)
                                           ttggTTTTTACCAGAACCCGGTTCTTTATTTTTTCCTAATTTTCCTCATTTTCCTTTTTCTTATTTTATATGCATGATAAATCATATTCGCGAGGATTATTTCTCTGCATTCCAATAAGGGAAAGGGAGTTAAGTGTGACAGTGGAGTTAAGTATGCCAGAGTTACAAACAGATCATCCAGAGACGGCGGAGTTAAGCAAGCCACAGCTACGCATGGTCGATCTCAACTTATTAACCGTTTTCGATgtcg                                                                                                                                                                                                       <  2:249260/247‑4 (MQ=255)
                                              gTTTTTATCAGAACCCGTATCTTTATGTTTTCCTAATTTTACTCATTTTCCTTTTTCTTATTTTATATGCATGATAAATCATATTCTCGAGGATTATTTCTCTGCATTCCAATAAGGGAAAGGGAGTTAAGTGTGACAGTGGAGTTAAGTATGCCATAGGTACAAACAGATCATCCAGAGACGGCGTAGTTAAGCAAGCCACAGCTACGCATGGTCGATCTCAACTTATTAACCGTTTTCGATGCCGTGAt                                                                                                                                                                                                   <  2:145684/251‑1 (MQ=255)
                                                                aTCTTTATGTTTTCCTAATTTTACTCATTTTCCTTTTTCTTATTTTATATGCATGATAAATAATATTCTCGAGGTTTATTTCTCTGCATTCCAATATGGGAAAGGAAGTTAAGTGTGACAGTGGAGTTAAGTATGCAAGAGGTACAAACAGATCATCCAGAGCCGGCGGAGTTAAGCAAGCCACAACTACGCATGGTCAACCTCAACTTATTAACCGTTTTCGATGCCGTGATGCAGGGGCAAAACATTat                                                                                                                                                                                 >  2:25029/1‑250 (MQ=255)
                                                                       tGTTTTCCTAATTTTACTCATTTTCCTTTTTTTTATTTTATATGCATGATAAATCATATTCTCGAGGATTATTTCTCTGCATTCCAATAAGGGAAAGGGAGTTAAGTGTGACAGGGGAGTTAAGTATGCCAGAGGTACAAACAGATCATCCAGAGACGGCGGAGTTAAGCAAGCCACAGCTACGCATGGTCGATCTCAACTTATTAACCGt                                                                                                                                                                                                                  <  2:354066/211‑1 (MQ=255)
                                                                       tGTTTTCCTAATTTTACTCATTTTCCTTTTTCTTATTTTATATGCATGATAAATCATATTCTCGAGGATTATTTCTCTGCATTCCAATAAGGGAAAGGGAGTTAAGTGTGACAGTGGAGTTAAGTATGCCAGAGGTACAAACAGATCATCCAGAGACGGCGGAGTTAAGCAAGCCACAGCTACGCATGGTCGATCTCAACTTATTAACCGt                                                                                                                                                                                                                  >  1:354066/1‑211 (MQ=255)
                                                                        gTTTTCCTAATTTTACTCCTTTTCCTTTTTCTTATTTTATATGCTTGATAAATCATCTTCTCGAGGATTCTTTCTCTGCATTCCAATAAGGGAAAGGGAGTTAAGTGTGACAGTGGAGTTAAGTATGCCAGAGGTACAAACAGATCATCCAGCGACGGCGGCGTTAAGCAAGCCACCGCTACGCATGGTCGATCTCAACTTCTTAACCGTTTTCGATGCCGTGATGCAGGGGCAAAACATTACTCGTGCCg                                                                                                                                                                         >  2:438287/1‑251 (MQ=255)
                                                                               taaTTTTACTCATTTTCCTTTTTCTTATTTTATATGCATGATAACTCATATTCTCGAGGATTATTTCTCTGCATTCCAATAAGGGAAAGGGAGTTAAGTGTGACAGTGGAGTTAAGTATGCCAGAGGAACAAACAGATCATCCAGAGACGGCGGAGTTAAGCAAGCCACAGCTACGCATGGTCGATCTCAACTTATTAACCGTTTTCGATGCCGTGTTGCAGGAGCAAAACATTACTCTTGCCGCTCATGt                                                                                                                                                                  >  1:289902/2‑251 (MQ=255)
                                                                                        tCATTTTCCTTTTTCTTATTTTATATGCATGATAAATCATATTCTCGAGGATTATTTCTCTGCATTCCAATAAGGGAAAGGGAGTTAAGTGTGACAGTGGAGTTAAGTATGCCAGAGGTACAAACAGATCATCCAGAGACGGCGGAGTTAAGCAAGCCACAGCTACGCATGGTCGATCTCAACTTATTAACCGTTTTCGATGCCGTGATGCAGGAGCAAAACATTACTCGTGCCGCTCATGTTCTGGGAAt                                                                                                                                                         >  1:77546/1‑251 (MQ=255)
                                                                                            tttccTTTTTCTTATTTTATATGCATGATAAATCATATTCTCGGGGATTATTTCTCTGCATTCCAATATGGGAAAGGGAGTTAAGTGTGACAGTGGAGTTAAGTATGCCAGAGGTACAAACAGATCATCCAGAGACGGCGGAGTTAAGCAAGCCACAGCTACGCATGGTCGATCTCAACTTATTAACCGTTTTCGATGCCGTGATGCAGGAGCAACACCTTACGCGTGCCGCTCATGTTCTGGGAATGTCg                                                                                                                                                     >  2:323639/5‑251 (MQ=255)
                                                                                                   tttCTTATTGTAAATGCATAATAAATCATATTATCGAGGATTATTTCGTTGAATTCCAATAAGGGAAAGGGAGTTAAGGGTGACAGTGGAGTTAAGTATGCCAGAGGTACAAACAGTTCACCCAGAGACGGCGGATTTAAGCAAGCCACAGCTACGCATGGTCGATCTCAACTTATTAACCGTTTTCGATGCCGTGATGCAGGAGCAAAACATTACTCGTGCCGCTCATGTTCTGGGAATGTCGCAACCt                                                                                                                                               <  2:168378/250‑1 (MQ=255)
                                                                                                       ttATTTTATATGCATGATAAATCATATTCTCGAGGATTATTTCTCTGCATTCCAATAAGGGAAAGGGAGTTAAGTGTGACAGTGGAGTTAAGTATGCCAGAGGTACAAACAGATCATCCAGAGACGGCGGAGTTAAGCAAGCCACAGCTACGCATGGTCGATCTCAACTTATTAACCGTTTTCGATGCCGTg                                                                                                                                                                                                     <  1:124826/192‑1 (MQ=255)
                                                                                                       ttATTTTATATGCATGATAAATCATATTCTCGAGGATTATTTCTCTGCATTCCAATAAGGGAAAGGGAGTTAAGTGTGACAGTGGAGTTAAGTATGCCAGAGGTACAAACAGATCATCCAGAGACGGCGGAGTTAAGCAAGCCACAGCTACGCATGGTCGATCTCAACTTATTAACCGTTTTCGATGCCGTg                                                                                                                                                                                                     >  2:124826/1‑192 (MQ=255)
                                                                                                         aTTTTATATGCATGATTAATCATATTCTTCAGGATTATTTCTCTGCATTCCAATAAGGGAAAGGGAGTTAAGTGTGACAGTGGAGTTAAGTATGCCAGAGGTACAAACAGATCATCCAGAGACGGCGGAGTTAAGCAAACCACAGCTACGCATGGTCGATCTCAACTTATTAACCGTTTTCGATGCCGTGATGCAGGAGCAAAACATTACTCGTGCCGCTCATGTTCTGGGAATGTCGCAACCTGCGGTc                                                                                                                                         <  2:89813/250‑1 (MQ=255)
                                                                                                         aTTTTATATGCATGATAAATCATATTCTCGAGGATTATTTCTCTGCATTCCAATAAGGGAAAGGGAGTTAAGTGTGAAAGTGGAGTTAAGTATGCCAGAGGTACAAACAGATCATCCAGAGACGGCGGAGTTAAGCAAGCCACAGCTACGCATGGTCGATCTCAACTTATTAACCGTTTTCGATGCCGTGATGCAGGAGCAAAACATTACTCGTGCCGCTCATGTTCTGGGAATGTCGCAACCTGCGGTc                                                                                                                                         >  1:190446/1‑250 (MQ=255)
                                                                                                             tataTGCATGATAAATCATATTCTCGAGGATTATTTCTCTGCATTCCAATAAGGGAAAGGGAGTTAAGTGTGACAGTGGAGTTAAGTATGCCAGAGGTACAAACAGATCATCCAGAGACGGCGGAGTTAAGCAAGCCACAGCTACGCATGGTCGATCTCAACTTATTAACCGTTTTCGATGCCGTGATGCAGGAGCAAAACATTACTCGTGCCGCTCATGTTCTGGGAATGTCGCAACCTGCGGTCAGTa                                                                                                                                     >  1:101256/1‑250 (MQ=255)
                                                                                                               tatGCATGATAAATCATATTCTCGAGGATTATTTCTCTGCATTCCAATAAGGGAAAGGGAGTTAAGTGTGACAGTGGAGTTAAGTATGCCAGAGGTACAAACAGATCATCCAGAGACGGCGGAGTTAAGCAAGCCACAGCTACGCATGGTCGATCTCAACTTATTAACCGTTTTCGATGCCGTGATGCAGGAGCAAAACATTACTCGTGCCGCTCCTGTTCTGGGAATGTCGCAACCTGCGGTCAGTAACg                                                                                                                                  >  1:211690/1‑251 (MQ=255)
                                                                                                                atGCATGATAAATCATATTCTCGAGGATTATTTCTCTGCCTTCCAATATGGGAAAGGTAGTTAAGTGTGACAGTGGAGTTAAGTATGCCAGAGGTACAAACAGATCATCCAGCGACGGCGGAGTTAAGCAAGCCACAACTACGCATGGTCGATCTCAACTTATTAACCTTTTTCGATGCCGTGATGCAGGAGCAAAACATTACTCGTGCCGACCATGTTCTGGGAATGTCGCAACCAGAGGTCAGTAACGc                                                                                                                                 >  2:295197/1‑251 (MQ=255)
                                                                                                                atGCATGATAAATCATATTCTCGAGGATTATTTCTCTGCATTCCAATAAGGGAAAGGGAGTTAAGTGTGACAGTGGAGTTAAGTATGCCAGAGGTACAAACAGATCATCCAGAGACGGCGGAGTTAAGCAAGCCACAGCTACGCATGGTCGATCTCAACTTATTAACCGTTTTCGATGCCGTGATGCAGGAGCAAAACATTACTCGTGCCGCTCATGTTCTGGGAATGTCGCAACCTGCGGTCAGTAACg                                                                                                                                  <  1:143743/250‑1 (MQ=255)
                                                                                                                 tGCATGATAAATCATATTCTCGAGGATTATTTCTCTGCATTCCAATAAGGGAAAGGGAGTTAAGTGTGACAGTGGAGTTAAGTATGCCAGAGGTACAAACAGATCATCCAGAGACGGCGGAGTTAAGCAAGCCACAGCTACGCATGGTCGATCTCAACTTATTAACCGTTTTCGATGCCGTGATGCAGGAGCAAAACATTACTCGTGCCGCTCATGTTCTGGGAATGTCGCAACCTGCGGTCAGTAACGCt                                                                                                                                >  1:43627/1‑251 (MQ=255)
                                                                                                                                     cgaGGATTATTTCTCTGCATTCCAATAAGGGAAAGGGAGTTAAGTGTGACAGTGGAGTTAAGTATGCCAGAGGTACAAACAGATCATCCAGAGACGGCGGAGTTAAGCAAGCCACAGCTACGCATGGTCGATCTCAACTTATTAACCGTTTTCGATGCCGTGATGCAGGAGCAAAACATTACTCGTGCCGCTCATGTTCTGGGAATGTCGCAACCTGCGGTCAGTAACGCTGTTGCACGCCTGAAGGTGAt                                                                                                            >  1:74213/3‑251 (MQ=255)
                                                                                                                                                        ttacAATAAGGGAAAGGACGTTAAGTGTGACAGTGGAGTTAAGTATGCCAGAGGTACAAACAGATCATCCAGAGACGGCGGAGTTAAGCAAGCCACAGCTACGCATGGTCGATCTCAACTTATTAACCGTTTTCGATGCCGTGATGCAGGAGCAAAACATTACTCGTGCCGCTCATGTTCTGGGAATGTCGCAACCTGCGGTCAGTAACGCTGTTGCACGCCTGAAGGTGATGTTTAATGACGAGCttttt                                                                                         >  2:330199/4‑251 (MQ=255)
                                                                                                                                                        ttCCAATAAGGGAAAGGGAGTTAAGTGTGACAGTGGAGTTAAGTATGCCAGAGGTACAAACAGATCATCCAGAGACGGCGGAGTTAAGCAAGCCACAGCTACGCATGGTCGATCTCAACTTATTAACCGTTTTCGATGCCGTGATGCAGGAGCAAAACATTACTCGTGCCGCTCATGTTCTGGGAATGTCGCAACCTGCGGTCAGTAACGCTGTTGCACGCCTGAAGGTGATGTTTAATGACGAGCttttt                                                                                         >  1:22634/1‑251 (MQ=255)
                                                                                                                                                          ccAATAAGGGAAAGGGAGTTAAGTGTGACAGTGGAGTTAAGTATGCCATAGGTACAAACAGATCATCCAGAGACGGCGGAGTTAAGCAAGCCACAGCTACGCATGGTCGATCTCAACTTATTAACCGTTTTCGATGCCGTGATGCAGGAGCAAAACATTACTCg                                                                                                                                                                              <  2:436300/164‑1 (MQ=255)
                                                                                                                                                          ccAATAAGGGAAAGGGAGTTAAGTGTGACAGTGGAGTTAAGTATGCCAGAGGTACAAACAGATCATCCAGAGACGGCGGAGTTAAGCAAGCCACAGCTACGCATGGTCGATCTCAACTTATTAACCGTTTTCGATGCCGTGATGCAGGAGCAAAACATTACTCg                                                                                                                                                                              >  1:436300/1‑164 (MQ=255)
                                                                                                                                                               aagggcaagggaGTTAAGTGTGACAGTGGAGTTAAGTATGACAGAGGTACAAACAGATCATCCAGAGACGGCGGAGTTAAGCAAGCCACAGCTACGCATGGTAGATATAAACTTATTAACCGTTTTCGCTGCCGTGATGAAGGAGCAAAACATTACTCGTGCCGCTCATGTTCTGGGCATGTCGCAACCTGCGGTACGTAACGATGTTGCACGCTTGCAGGGGATGTTTAATGAAGAGCTTTTTGTTGGtt                                                                                  >  2:440492/1‑251 (MQ=255)
                                                                                                                                                               aagggaaagggaGTTAAGTGTGACAGTGGAGTTAAGTATGCCAGAGGTACAAACAGATCATCCAGAGACGGCGGAGTTAAGCAAGCCACAGCTACGCATGGTCGATCTCAACTTATTAACCGTTTTCGATGCCGTGATGCAGGAGCAAAACATTACTCGTGCCGCTCATGTTCTGGGAATGTCGCAACCTGCGGTCAGTAACGCTGTTGCACGCCTGAAGGTGATGTTTAATGACGAGCTTTTTGTTCGtt                                                                                  <  1:18207/251‑1 (MQ=255)
                                                                                                                                                                  agaaagggaGTTAAGTGTGACCGAGGAGTTAAGAATGCCAGAAGTACAAACCGATCATCCAGAGACGGAGGAGTTAAGCAAGCCACAGCTACGAATGATCGATCTCAACTTATTACCAGTTTTAGATGCCCTGATGCCGGAACAAACCATTACTCGTGCCGCTCATGTTATGGGAATGTCGCAACTTGCGGTCAGTAACGCTGTCGCACGCCTGAAGGTGATGTTTAATGACGAGCTTTTTGTACGttg                                                                                 >  2:362854/2‑248 (MQ=255)
                                                                                                                                                                   gaaagggaGTTAAGTGGGACAGTGGAGTTAAGTATGCCAGAGGTACAATCAGATCAACCAGAGACGGCGGAGTTAAGCAAGCCACAGCTACGCATGGGCGATCTCAACTTATTAACCGTTTTCGATGCCGTGATGCAGGAGCAAAACATTACTCGGGCCGCTCATGTTCTGGGAATGTCGCAACCTGCGTTCAGTAACGCTGTTGCACGCCTGAAGGTGATGTTTAATGACGAGCTTTTTGTTCGTTATgg                                                                              <  1:435186/251‑1 (MQ=255)
                                                                                                                                                                         gaGTTAAGTGTGACAGTGGAGTTAAGTATGCCAGAGGTACAAACAGATCATCCAGAGACGGCGGAGTTAAGCAAGCCACAGCTACGCATGGTCGATCTCAACTTATTAACCGTTTTCGATGCCGTGATGCAGGAGCAAAACATTACTCGTGCCGCTCATGTTCTGGGAATGTCGCATCCTGCGGTCAGTAACGCTGTTGCACGCCTGAAGGTGATGTTTAATGACGAGCTTTTTGTTCGTTATGGCCGTgg                                                                        <  2:101256/251‑1 (MQ=255)
                                                                                                                                                                             tAAGTGTGACAGTGGAGTTAAGTATGCCAGAGGTACAAACAGATCATCCAGAGACGGCGGAGTTAAGCAAGCCACAGCTACGCATGGTCGATCTCAACTTATTAACCGTTTTCGATGCCGTGATGCAGGAGCAAAACATTACTCGTGCCGCTCATGTTCTGGGAATGTCGCAACCTGCGGTCAGTAACGCTGTTGCACGCCTGAAGGTGATGTTTAATGACGAGCTTTTTGTTCGTTATGGCCGTGGTAtt                                                                    >  1:385860/1‑251 (MQ=255)
                                                                                                                                                                                          ggAGTTAAGTATGCCAGAGGTACAAACAGATCATCCAGAGACGGCGGAGTTAAGCAAGCCACAGCTAAGCATGGTCGATCTCAACTTATTAACCGTTTTCGATGCCGTGATGCAGGAGCAAAACATTACTCGTGACGCTCATGTTCTGGGAATGTCGCAACCTGCGGTCAGTAACGCTGTTGCACGACTGAAGGTGATGGTAAATGACGAGCTTTTTGTTCGTTTTGGCCGTGGTATTCacacgacttct                                                        >  2:379188/1‑240 (MQ=255)
                                                                                                                                                                                             gTTAAGTATGCCAGAGGTACAAACAGATCATCCAGAGACGGCGGAGTTAAGCAAGCCACAGCTACGCATGGTCGATCTCAACTTATTAACCGTTTTCGATGCCGTGATGCAGGAGCAAAACATTACTCGTGCCGCTCATGTTCTGGGAATGTCGCAACCTGCGGTCAGTAACGCTGTTGCACGCCTGAAGGTGATGTTTAATGACGAGCTTTTTGTTCGTTATGGCCGTGGTATTCAACCGACTGCTcgcg                                                    >  1:242725/1‑251 (MQ=255)
                                                                                                                                                                                                            ggTACAAACAGATCATCCAGAGACGGCGGAGTTAAGCAAGCCACAGCTACGCATGGTCGATCTCAACTTATTAACCGTTTTCGATGCCGTGATGCAGGAGCAAAACATTACTCGTGCCGCTCATGTTCTGGGAATGTCGCAACCTGCGGTCAGTAACGCTGTTGCACGCCTGAAGGTGATGTTTAATGACGAGCTTTTTGTTCGTTATGGCCGTGGTATTCAACCGACTGCTCGCGCATTTCAACTTTTTg                                     >  2:115838/1‑251 (MQ=255)
                                                                                                                                                                                                                         catcCAGAGACGGCGGAGTTAAGCAAGCCACAGCTACGCATGGTCGATCTCAACTTATTAACCGTTTTCGATGCCGTGATGCAGGAGCAAAACATTACTCGTGCCGCTCATGTTCTGGGAATGTCGCAACCTGCGGTCAGTAACGCTGTTGCACGCCTGAAGGTGATGTTTAATGACGAGCTTTTTGTTCGTTATGGCCGTGGTATTCAACCGACTGCTCGCGCATTTCAACTTTTTGGTTCAGTTCGTct                        >  2:164586/1‑250 (MQ=255)
                                                                                                                                                                                                                                               gCAAGCCACAGCTAAGCATGGTCGATCTCAACTGATTAACCGTTTTCGATGCCGTGATGCAGGAGCAAAACATTACTCGTGCCGCTCATGTTCTGGGAATGGCGCAACATGCGGTCAGTAACGCTGTTGCACGCCTGAAGGTGATGTTTAATGACGAGCTTTTTGTTCGTTATGGCCGTGGTATTCAACCGACTGCTCGCGCATTTCAACTTTTTGGTTCAGTTCGTCAGGCATTGCAACTAGTACAAAAt  <  2:275104/251‑1 (MQ=255)
                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                      
GATTATTATGCTGTGGTAAATGACTCATTCCACGGCAATGGATTCTGTTTTTATCAGAACCCGTATCTTTATGTTTTCCGAATTTTACTCATTTTGCTTTTTCTTATTTTATATGCATGATAAATCATATTCTTCAGGATTATTTCTCTGCATTCCAATAAGGGAAAGGGAGTTAAGTGTGACAGTGGAGTTAAGTATGCCAGAGGTACAAACAGATCATCCAGAGACGGCGGAGTTAAGCAAACCACAGCTACGCATGGTCGATCTCAACTTATTAACCGTTTTCGATGCCGTGATGCAGGAGCAAAACATTACTCGTGCCGCTCATGTTCTGGGAATGTCGCAACCTGCGGTCAGTAACGCTGTTGCACGCCTGAAGGTGATGTTTAATGACGAGCTTTTTGTTCGTTATGGCCGTGGTATTCAACCGACTGCTCGCGCATTTCAACTTTTTGGTTCAGTTCGTCAGGCATTGCAACTAGTACAAAAT  >  NC_000913/84172‑84661

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑