| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 4,154,371 | G→A | A159A (GCC→GCT) | argE ← | acetylornithine deacetylase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,154,371 | 0 | G | A | 95.1% | 170.2 / ‑1.8 | 62 | A159A (GCC→GCT) | argE | acetylornithine deacetylase |
| Reads supporting (aligned to +/- strand): ref base G (1/2); new base A (26/32); total (27/35) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.13e-01 | |||||||||||
AGGTTGAGCGTAGGGTATGGCACGGTAAACGCTTCGTAGTGATAACGTTCTTTCAGGTTATCGCGCAATTGCAAAATATGCCCGATGGCGTCGTGCATTAGTTCGATAGCGTTAACTCCGCGTGCTGGATCGCTGGAGTGCCCCGACTGGCCCTGAATACGGATGGCGTTAGAGATATGACCTTTATGTGCGCGTACCGGTTGTAGTGACGTCGGTTCGCCAATGATGGCGCAATCCGGGCGCAGGGCGGTAGTTTCGGCAAAATAACGCGCTCCGGCCA‑TACTGGTTTCTTCATCAGCAGTCGCCAGAATGTAGAGCGGTTTTTTCAGTTTCGTGACGTCGACATCGCGTAGCGCATCAAGGATAAACGCAAAAAAGCCTTTCATGTCGGCGGTGCCTAAGCCGTAAAGCTTGCCGTCATGCTCCGTCAGTGTAAACGGATCGCGCGTCCAGCGACCGTCATCAAATGGCACCGTATCGGTATGCCCCGCC > NC_000913/4154126‑4154617 | aggctGAGAGTAGGGAATGGCACGGTAACCGCTTCGTAGTGATAACGTTCTTTCAGGTTATCGCGCACTTGCAAAATATGCCCGATGGAGTCGTGCATTAGTTCGATAGCGTTAACTCCGCGCGCTGGATCGCTGGCGTGCCCCGACTGGCCCTGAATCCGGATGGCGTTAGAGATATGACCTTTATGTGCGCGTCCAGGTTGTCGGGACGTAGGTTCGCCAATTATTGCGCAATCAGGGCGCAGAGCGGt > 2:399662/5‑251 (MQ=255)aGGTTGAGCGTAGGGTATGGCACGGTAAACGCTTCGTAGTGATAACGTTCTTTCAGGTTATCGCGCAATTGCAAAATATGCCCGATGGCGTCGTGCATTAGTTCGATAGCGTTAACTCCGCGTGCTGGATCGCTGGAGTGCCCCGACTGGCCCTGAATACGGATGGCGTTAGAGATATTACCTTTATGTGCGCGTACCGGTTGTAGTGACGTCGGTTCGCCCATGATGGCGCAATCCGGGCGCAGGGCGGt > 2:49679/1‑251 (MQ=255)aGGTTGAGCGTAGGGTATGGCACGGTAAACGCTTCGTAGTGATAACGTTCTTTCAGGTTATCGCGCAATTGCAAAATATGCCCGATGGCGTCGTGCATTAGTTCGATAGCGTTAACTCCGCGTGCTGGATCGCTGGAGTGCCCCGACTGGCCCTGAATACGGATGGCGTTAGAGATATGACCTTTATGTGCGCGTACCGGTTGTAGTGACGTCGGTTCGCCAATGATGGCGCAATCCGGGCGCAGAGCGGt < 2:90830/251‑1 (MQ=255) gaacggaGGGTATGGCACGGTAAACGCTTCGTAGTGATAACGTTCTTTCAGGTTATCGCGCAATTGCAAAATATGCCCGATGGCGTCGTGCATTAGTTCGATAGCGTTAACTCCGCGTGCTGGATCGCTGGAGTGCCCCGACTGGCCCTGAATACGGATGGCGTTAGAGATATGACCTTTATTTGCGCGTACCGGTTGTAGGGACGCCGGTTCGCCACTACTGGCGCAATCCGGGCGCAGAGCGGTAGttt > 2:144059/7‑251 (MQ=255) gAGCGTAGGGTATGGCACGGTAAACGCTTCGTAGTGATAAAGTTCTTTCAGGTTATCGCGCAATTGCAAAATATGCCCGATGGCGTCGTGCATTAGTTCGATAGCGTTAACTCCGCGTGCTGGATCGCTGGAGTGCCCCGACTGGCCCTGAATCCGGATGGCGTTCGAGATATGACCTTTATGTGCGCGTACCGGTTGTAGTGACGTCGGTTAGCCAATGATGGCGCAATCCGGGCGCCGAGAGGTAGttt > 2:437232/1‑251 (MQ=255) aGCGTAGGGTATGGCACGGTAAACGCTTCGTAGTGATAACGTTCTTTCAGGTTATCGCGCAATTGCAAAATATGCCCGATGGCGTCGTGCATTAGTTCGATAGCGTTAACTCCGCGTGCTGGATCGCTGGAGTGCCCCGACTGGCCCTGAATACGGATGGCGTTAGAGATATGACCTTTATGTGCGCGTACCGGTTGTAGTGACGTCGGTTCGCCAATGATGGCGCAATCCGGGCGCAGAGCGGTAGTTTc < 1:389031/251‑1 (MQ=255) aGGGTATGGCACGGTAAACGCTTCGTAGTGATAACGTTCTTTCAGGTTATCGCGCAATTGCAAAATATGCCCGATGGCGTCGTGCATTAGTTCGATAGCGTTAACTCCGCGTGCTGGATCGCTGGAGTGCCCCGACTGGCCCTGAATACGGATGGCGTTAGAGATATGACCTTTATGTGCGCGTACCGGTTGTAGTGACGTCGGTTCGCCAATGATGGCGCAATCCGGGCGCAGAGCGGTAGTTTCGGCa > 2:160939/1‑250 (MQ=255) gTATGGCACGGTAAACGCTTCGTAGTGATAACGTTCTTTCAGGTTATCGCGCAATTGCAAAATATGCCCGATGGCGTCGTGCATTAGTTCGATAGCGTTAACTCCGCGTGCTGGATCGCTGGAGTGCCCCGACTGGCCCTGAATACGGATGGCGTTAGAGATATGACCTTTATGTGCGCGTACCGGTTGTAGTGACGTCGGTTCGCCAATGATGGCGCAATCCGGGCGCAGAGCGGTAGTTTCGGCAAAAt < 1:217783/251‑1 (MQ=255) cGGTAAACGCTTCGTAGTGATAACGTTTTTTCTGGTTATCGCGCAATTGCAAAATATGCCCGATGGCGTCTTGCATTAGTTCGATAGCGTTAACTCCGCGTGCTGGATCGCTGTAGTGCCCCGACTGGCCCTGAATACGGATGGCGTTAGAGATATGAACTTTATGTGCGCGTACCGGTTGTAGTGACGTCGGTTCGCCAATGATGGCGCAATCCGGGCGCAGAGCGGTAGTTTCGGCAAAATACCGCGCt < 2:382835/251‑1 (MQ=255) gTAAATGCTTCGTAGTGATAACGTTCTTTCAGGTTATCGCGCAATTGCAAAATATGCCCGATTGCGTCGTGCATTAGTTCGATAGCGTTAACTCCGCGTGCTGGCTCGCTGGAGTGACCCGACTGGCACTGAATACGGATGGCGTTAGAGATATGTCCTTTATGTGCGCGTACCGGTTGTAGTGACGTCGGTTCGCCAATGATGGCGCACTCCGGGGGCAGAGCGGGAGGTTCGTCCAAATAACGCGCTcc > 2:127117/1‑251 (MQ=255) tAGTGATAACGTTCTTTCAGGTTATCGCGCAATTGCAAAATATGCCCGATGGCGTCGTGCATTAGTTCGATAGCGTTAACTCCGCGTGCTGGATCGCTGGAGTGCCCCGACTGGCCCTGAATACGGATGGCGTTAGAGATATGACCTTTATGTGCGCGTACCGGTTGTAGTGACGTCGGTTCGCCAATGATGGCGCAATCCGGGCGCAGAGCGGTAGTTTCGGCAAAATAACGCGCTCCGGCCA‑TACTGGt > 1:110484/1‑251 (MQ=255) aaCGTTCTTTCAGGTTATCGCGCAATTGCAAAATATGCCCGATGGCGTCGTGCATTAGTTCGATAGCGTTAACTCCGCGTGCTGGATCGCTGGAGTGCCCCGACTGGCCCTGAATACGGATGGCGTTAGAGATATGACCTTTATGTGCGCGTACCGGTTGTAGTGACGTCGGTTCGCCAATGATGGCGCAATCCGGGCGCAGAGCGGTAGTTTCGGCAAAATAACGCGCTCCGGCCA‑TACTGGTttcttc > 1:266012/1‑250 (MQ=255) cGTTCTTTCAGGTTATCGCGCAATTGCAAAATATGCCCGATGGCGTCGTGCATTAGTTCGATAGCGTTAACTCCGCGTGCTGGATCGCTGGAGTGCCCCGACTGGCCCTGAATACGGATGGCGTTAGAGATATGACCTTTATGTGCGCGTACCGGTTGTAGTGACGTCGGTTCGCCAATGATGGCGCAATCCGGGCGCAGAGCGGTAGTTTCGGCAAAATAACGCGCTCCGGCCA‑Ta > 2:428373/1‑237 (MQ=255) cGTTCTTTCAGGTTATCGCGCAATTGCAAAATATGCCCGATGGCGTCGTGCATTAGTTCGATAGCGTTAACTCCGCGTGCTGGATCGCTGGAGTGCCCCGACTGGCCCTGAATACGGATGGCGTTAGAGATATGACCTTTATGTGCGCGTACCGGTTGTAGTGACGTCGGTTCGCCAATGATGGCGCAATCCGGGCGCAGAGCGGTAGTTTCGGCAAAATAACGCGCTCCGGCCA‑Ta < 1:428373/237‑1 (MQ=255) gTTCTTTCAGGTTATCGCGCAATTGCAAAATATGCCCGATGGCGTCGTGCATTAGTTCGATAGCGTTAACTCCGCGTGCTGGATCGCTGGAGTGCCCCGACTGGCCCTGAATACGGATGGCGTTAGAGATATGACCTTTATGTGCGCGTACCGGTTGTAGTGACGTCGGTTCGCCAATGATGGCGCAATCCGGGCGCAGAGCGGTAGTTTCGGCAAAATAACGCGCTCCGGCCA‑TACTGGTTTCTtcatca < 1:160939/251‑1 (MQ=255) cTTTCAGGTTATCGCGCAATTGCAAAATATGCCCGATGGCGTCGTGCATTAGTTCGATAGCGTTAACTCCGCGTGCTGGATCGCTGGAGTGCCCCGACTGGCCCTGAATACGGATGGCGTTAGAGATATGACCTTTATGTGCGCGTACCGGTTGTAGTGTCGTCGGTTCGCCAATGATGGCGCAATCCGGGCGCAGAGCGGTAGTTTCGGCAAAATAACGCGCTCCGGCCA‑TACTGGTTTCTTCATcagcc > 1:299460/1‑250 (MQ=255) cTTTCAGGTTATCGAGCAATTGCAAAATATGCCCGCTGGCGTCGTGCATTAGTTCGATAGCGTTAACTCCGCGTGCTGGATCGCTGGAGTGCCCAGACTGGCCCTGAATACGGATGGCGTTAGAGATATGACCTTTATGTGCGCGTACCGGTTGTAGTGACGTCGGTTCGCCAATGATGGCGCAATCCGGGCGCAGAGCGGTAGTTTCGGCAAAATAACGCGCTCAGGCCA‑TACTGGCTTCTTCATcagc > 2:201507/1‑250 (MQ=255) tATCACGCAATTGCAAAATATGCCCGATGGCATCGTGCATTAGTTCGATAGCGTTAACTCCGCGTGCTGGATCGATGGAGTGCCCCGCCTGGCACTTACTACGGAAAACGTTAGCGATAAGGCCTTTATGGGCGCGTACCGGTTGTAGTGAAGTCGGGTCACCAATAATGTCGAAATCCGGGTGCAGAGCGGTAGATTCGGCAAAATAACGCGCTCCGGCCAATATTGGTT‑CTTCATcagcgatcgccg > 2:405147/1‑241 (MQ=255) tCGCCCAATTGCAAAATATGCCCGATGGCGTCGCGCATTAGTTCGATAGCGTTAACTCCGCGTGCTGGATCGCTGGAGTGCCCCGACTGGCCCTGAATACGGATGGCGTTAGAGATATGACCTTTATGTGCGCGTACCGGTTGTAGTGACGTCGGTTCGCCAATGATGGCGCAATCCGGGCGCAGAGCGGTAGGTTCGGCAAAATAACGCGCTCCGGCCA‑TACTGGCTTCTTCATCAGCAGTCGCCAGAAt > 2:176699/1‑251 (MQ=255) aTTGCAAAATATGCCCGATGGCGTCGTGCATTAGTTCGATAGCGTTAACTCCGCGTGCTGGATCGCTGGAGTGCCCCGACTGGCCCTGAATACGGATGGCGTTAGAGATATGACCTTTATGTGCGCGTACCGGTTGTAGTGACGTCGGTTCGCCAATGATGGCGCAATCCGGGCGCAGAGCGGTAGTTTCGGCAAAATAACGCGCTCCGGCCA‑TACTGGTTTCTTCATCAGCAGTCGCCAGAATGTAGAGc > 1:104161/1‑251 (MQ=255) gCAAAATATGCCCGGGGGCGTCGTGCTTTAGTTCGAGAGCGTTAACTCCGCGTGCTGGAGCGCTGGAGTGCCCCGACTGGCCCTGAATACGGATGGCGTTAGAGATATGACCTTTATGTGCGCGTACCGGTTGTAGTGACGTCGGTTCGCCAATGATGGCGCAATCCGGGCGCAGAGCGGTAGTTTCGGCAAAATAACGCGCTCCGGCCA‑TACTGGTTTCTTCATCAGCAGTCGCCAGAATGTAGAGCGGt < 2:339080/251‑1 (MQ=255) atGCCCGATGGCGTCGTGCATTAGTTCGATAGCGTTAACTCCGCGTGCTGGATCGCTGGAGTGCCCCGACTGGCCCTGAATACGGATGGCGTTAGAGATATGACCTTTATGTGCGCGTACCGGTTGTAGTGACGTCGGTTCGCCAATGATGGCGCAATCCGGGCGCAGAGCGGTAGTTTCGGCAAAATAACGCGCTCCGGCCA‑TACTGGTTTCTTCATCAGCAGTCGCCAGAATGTAGAGCGGTTTTTTCa < 1:439383/251‑1 (MQ=255) gCCCGATGGCGTCGTGCATTAGTTCGATAGCGTTAACTCCGCGTGCTGGATCGCTGGAGTGCCCCGACTGGCCCTGAATACGGATGGCGTTAGAGATATGACCTTTATGTGCGCGTACCGGTTGTAGTGACGTCGGTTCGCCAATGATGGCGCAATCCGGGCGCAGAGCGGTAGTTTCGGCAAAATAACGCGCTCCGGCCA‑TACTGGTTTCTTCATCCGCAGTCGCCAGAATGTAGAGCGGTTTTTTCAGt > 2:413721/1‑251 (MQ=255) gtcgtGCATTAGTTCGATAGCGTTAACTCCGCGTGCTGGATCGCTGGAGTGCCCCGACTGGCCCTGAATACGGATGGCGTTAGAGATATGACCTTTATGTGCGCGTACCGGTTGTAGTGACGTCGGTTCGCCAATGATGGCGCAATCCGGGCGCAGAGCGGTAGTTTCGGCAAAATAACGCGCTCCGGCCA‑TACTGGTTTCTTCATCAGCAGTCGCCAGAATGTAGAGCGGTTTTTTCAGTTTCGTGACGt > 1:392490/1‑251 (MQ=255) cgtGCATTAGTTCGATAGCGTTAACTCCGCGTGCTGGATCGCTGGAGTGCCCCGACTGGCCCTGAATACGGATGGCGTTAGAGATATGACCTTTATGTGCGCGTACCGGTTGTAGTGACGTCGGTTCGCCAATGATGGCGCAATCCGGGCGCAGAGCGGTAGTTTCGGCAAAATAACGCGCTCCGGCCA‑TACTGGTTTCTTCATCAGCAGTCGCCAGAATGTagag < 2:293267/226‑1 (MQ=255) cgtGCATTAGTTCGATAGCGTTAACTCCGCGTGCTGGATCGCTGGAGTGCCCCGACTGGCCCTGAATACGGATGGCGTTAGAGATATGACCTTTATGTGCGCGTACCGGTTGTAGTGACGTCGGTTCGCCAATGATGGCGCAATCCGGGCGCAGAGCGGTAGTTTCGGCAAAATAACGCGCTCCGGCCA‑TACTGGTTTCTTCATCAGCAGTCGCCAGAATGTagag > 1:293267/1‑226 (MQ=255) cgtGCATTAGTTCGATAGCGTTAACTCCGCGTGCTGGATCGCTGGAGTGCCCCGACTGGCCCTGAATACGGATGGCGTTAGAGATATGACCTTTATGTGCGCGTACCGGTTGTAGTGACGTCGGTTCGCCAATGATGGCGCAATCCGGGCGCAGAGCGGTAGTTTCGGCAAAATAACGCGCTCCGGCCA‑TACTGGTTTCTTCATCAGCAGTCGCCAGAATGTAGAGCGGTTTTTTCAGTTTCGTGACGTCg > 1:443403/1‑251 (MQ=255) gtGCATTAGTTCGATAGCGTTAACTCCGCGTGCTGGATCGCTGGAGTGCCCCGACTGGCCCTGAATACGGATGGCGTTAGAGATATGACCTTTATGTGCGCGTACCGGTTGTAGTGACGTCGGTTCGCCAATGATGGCGCAATCCGGGCGCAGAGCGGTAGTTTCGGCAAAATAACGCGCTCCGGCCA‑TACTGGTTTCTTCATCAGCAGTCGCCAGAATGGAGAGCGGTTTTTTCCGTTTCGTGGCGTCgg > 1:238086/1‑250 (MQ=255) aTTAGTTCGATAGCGTTAACTCCGCGTGCTGGATCGCTGGAGTGCCCCGACTGGCCCTGAATACGGATGGCGTTAGAGATATGACCTTTATGTGCGCGTACCGGTTGTAGTGACGTCGGTTCGCCAATGATGGCGCAATCCGGGCGCAGGGCGGTAGTTTCGGCAAAATAACGCGCTCCGGCCA‑TACTGGTTTCTTCATCAGCAGTCGCCAGAATGTAGAGCGGTTTTTTCAGTTTCGTGACGTCGACATc < 2:177098/251‑1 (MQ=255) aggtcgagagcggtaaacccGCGTGCTGGATCGCTGGAGTGCCCCGACTGGCCCTGAAGACGGATGGCGTTAGAGGTAGTACCTTTATGTGCGCGGACCGGTGGTAGGGACGTCGGTTCGCCAATGATGGCGCAATCCGGGCGCAGAGCGGTAGTTTCGGCAAAATAACGCGCTCCGGCCA‑TACTGGTTTCTTCATCAGCAGTCGCCAGAAAGTAGAGCGGTTTTTTCAGTGGCGTGACGTCGACATcgcg < 2:290123/233‑1 (MQ=255) ttCGATAGCGTTAACTACGCGTGCTGGATCGCTGGAGTGCCCCGACTGGCCCTGAATACGGCTGGCGTTAGAGATATGACCTTTATGTGCGCGTACCGGTTGTAGTGACGTCGGTTCGCCAATGATGGCGCAATCCGGGCGCAGAGCGGTAGTTTCGGCAAAATAACGCGCTCCGGCCA‑TACTGGGTTCTTCTTCCGCAGTCGCCATAATGTAGAGCGGTTTTTTCAGTTTCGTGACGTCGACATCGCGtt > 2:112717/1‑250 (MQ=255) cGATAGCGTTAACTCCGCGTGCTGGATCGCTGGAGTGCCCCGACTGGCCCTGAATACGGATGGCGTTAGAGATATGACCTTTATGTGCGCGTACCGGTTGTAGTGACGTCGGTTCGCCAATGATGGCGCAATCCGGGCGCAGAGCGGTAGTTTCGGCAAAATAACGCGCTCCGGCCA‑TACTGGTTTCTTCATCAGCAGTCGCCAGAATGTAGAGCGGTTTTTTCAGTTTCGTGACGTCGACATCGCGTAg < 1:96464/250‑1 (MQ=255) tAACTCCGCGGGCTGGATCGCTGGAGTGCCCCGACTGGCCCTGAATACGGAGGGAGTTAGAGATATGACCTTTATGTGCGCGTACCGGTTGTAGTGACGTCGGTTCGCCAATGATGGCGCAATCCGGGCGCAGAGCGGTAGTTTCGGCAAAATAACGCGCTCCGGCCA‑TACTGGTTTCTTCATCAGCAGTCGCCAGAATGTAGAGCGGTTTTTTCAGTTTCGTGACGTCGACATCGCGTAGCGCATCAAg < 2:370315/250‑1 (MQ=255) aCTCCGCGTGCTGGATCGCTGGAGTGCCCCGACTGGCCCTGAATACGGATGGCGTTAGAGATATGACCTTTATGTGCGCGTACCGGTTGTAGTGACGTCGGTTCGCCAATGATGGCGCAATCCGGGCGCAGAGCGGTAGTTTCGGCAAAATAACGCGCTCCGGCCA‑TACTGGTTTCTTCATCAGCAGTCGCCAGAATGTAGAGCGGTTTTTTCAGTTTCGTGACGTCGACATCGCGTAGCGCATCAAGGAt < 1:437232/251‑1 (MQ=255) ccGCGTGCTGGATCGCTGGAGTGCCCCGACGGGCCCTGAATACGGATGGCGTTAGAGATATGACCTTTATGGTCGCGTACCGGTTGTAGTGACGTCGGTTCGCCAATGATGGCGCAATCCGGGCGCAGAGCGGTAGTTTCGGCAAAATAACGCGCTCCGGCCA‑TACTGGTTTCTTCATCAGCAGTCGCCAGAATGTAGAGCGGTTTTTTCAGTTTCGTGACGTCGACATCGCGTAGCGCATCAAGGATaaa < 2:392490/251‑1 (MQ=255) gcggtatGGATTGCTGGAGTGCCCCGAATGGCCCTGAATACGTATGGCGTTAGAGATATGACCTTTATGTGCGCGTACCGGTTTTAGTGACGTCGGTTCGCCAATGATGGCGCAAGCCGGGCGCAGAGCGGTAGGTTCGGCAAAAGAACGCGCTCCGGCCA‑TACTGGTTTCTTCAGCAGCAGTCGCCAGAATGTAGAGCGGTTTTTTAAGTTTCGGGACGTCGACATCGCGTAGCGCATCAAGTATaaa < 2:298443/243‑1 (MQ=255) gatcggtgtagggCCCCTACTGGCACTGAATACGGATGGCGTTAGTGATATGACTTTTATGTGCTGGTTCCGGTTGTAGTGTCGTCGGTTCGCCTATGATGGCGCAATCCGGGCGCAGAGCGGTAGTTTCGGCAAAATAACGCGCTCCGGCCA‑TACTGGTTGCTTCATCAGCAGTCGCCAGAATGTAGAGCGGTTTTTTCAGTTTCATGACGTCGACATCGCGGAGCGCATCAAGGATAAACGCAAAAAAg < 2:251900/239‑1 (MQ=255) cGCTGGAGTGCCCCGACTGGCCCTGAATACGGATGGCGTTAGAGATATGACCTTTATGTGCGCGTACCGGTTGTAGTGACGTCGGTTCGCCAATGATGGCGCAATCCGGGCGCAGAGCGGTAGTTTCGGCAAAATAACGCGCTCCGGCCA‑TACTGGTTTCTTCATCAGCAGTCGCCAGAATGTAGAGCGGTTTTTTCAGTTTCGTGACGTCGACATCGCGTAGCGCATCAAGGATAAACGCAAAAAAGcc < 2:110484/250‑1 (MQ=255) gCTGGAGTGCCCCGACTGGCCCTGAATACGGATGGCGTTAGAGATATGACCTTTATGTGCGCGTACCGGTTGCAGTGACGTCGGTTCGCCAATGATGGCGCAATCCGGGCGCAGAGCGGTAGTTTCGGCAAAATAACGCGCTCCGGCCA‑TACTGGTTTCTTCATCAGCAGTCGCCAGAATGTAGAGCGGTTTTTTCAGTTTCGTGACGTCGACATCGCGTAGCGCATCAAGGATAAACGCAAAAAAGCCtt < 1:20200/251‑1 (MQ=255) gCCCCGTCTGGCCCTGAATACGGATGGCGTTAGAGATATGACCTTTATGTGCGCGTACCGGTTGTAGTGACGGCGGTTCGCCAATGATGGCGCAATCCGGGCGCAGAGCGGTAGTTTCGGCAAAATAACGCGCTCCGGCCA‑TACTGGTTTCTTCATCAGCAGTCGCCAGAATGTAGAGCGGTTTTTTCAGTTTCGTGACGTCGACATCGCGTAGCGCATCAAGGATAAACGCAAAAAAGCCTTTCATGTcg < 2:343042/251‑1 (MQ=255) ccccGACTGGCCCTGAATACGGATGGCGTTAGAGATATGACCTTTATGTGCGCGTACCGGTTGTAGTGACGTCGGTTCGCCAATGATGGCGCAATCCGGGCGCAGAGCGGTAGTTTCGGCAAAATAACGCGCTCCGGCCA‑TACTGGTTTCTTCATCAGCAGTCGCCAGAATGTAGAGCGGTTTTTTCAGTTTCGTGACGTCGACATCGCGTAGCGCATCAAGGATAAACGCAAAAAAGCCTTTCATGTcgg < 1:116026/251‑1 (MQ=255) cggcTGGCCCTGAATACGGTTGTCGTTAGAGATATGACCTTTAGGTGCGCGTACCGGTTGTAGTGACGTCGGTTCGCCAATGATGGTGCAATCCGGGCGCAGGGCGGTAGTTTCGGCAAAATAACGCGCTCCGGCCA‑TACTGGTTTCTTCAGCAGCAGTCGCCAGAATGTAGAGCGGTTTTTTCAGTTTCGTGACGTCGACATCGCGTAGCGCATCAAGGATAAACGCAAAAAAGCCTTTCATGTcggcgg < 2:325843/248‑1 (MQ=255) gACTGGCCCTGAATACGGATGGCGTTAGAGATATGACCTTTATGTGCGCGTACCGGTTGTAGTGACGTCGGTTCGCCAATGATGGCGCAATCCGGGCGCAGAGCGGTAGTTTCGGCAAAATAACGCGCTCCGGCCA‑TACTGGTTTCTTCATCAGCAGTCGCCAGAATGTAGAGCGGTTTTTTCAGTTTCGTGACGTCGACATCGCGTAGCGCATCAAGGATAAACGCAAAAAAGCCTTTCATGTCGGCGGt < 1:349815/251‑1 (MQ=255) ccTGAATACGGATGGCGTTAGAGATATGACCTTTATGTGCGCGTACCGGTTGTAGTGACGTCGGTTCGCCAATGATGGCGCAATCCGGGCGCAGAGCGGTAGTTTCGGCAAAATAACGCGCTCCGGCCA‑TACTGGTTTCTTCATCAGCAGTCGCCAGAATGTAGAGCGGTTTTTTCAGTTTCGTGACGTCGACATCGCGTAGCGCATCAAGGATAAACGCAAAAAAGCCTTTCATGTCGGCGGTGCCTAAg < 1:333636/251‑1 (MQ=255) ccTGAATACGGATGGCGTTAGAGATATGACCTTTATGTGCGCGTACCGGTTGTAGTGACGTCGGTTCGCCAATGATGGCGCAATCCGGGCGCAGAGCGGTAGTTTCGGCAAAATAACGCGCTCCGGCCA‑TACTGGTTTCTTCATCAGCAGTCGCCAGAATGTAGAGCGGTTTTTTCAGTTTCGTGACGTCGACATCGCGTAGCGCATCAAGGATAAACGCAAAAAAGCCTTTCATGTCGGCGGTGCCTAAg < 1:368005/251‑1 (MQ=255) gAATACGGATGGCGTTAGAGATATGACCTTTATGTGCGCGTACCGGTTGTAGTGACGTCGGTTCGCCAATGATGGCGCAATCCGGGCGCAGAGCGGTAGTTTCGGCAAAATAACGCGCTCCGGCCA‑TACTGGTTTCTTCATCAGCAGTCGCCAGAATGTAGAGCGGTTTTTTCAGTTTCGTGACGTCGACATCGCGTAGCGCATCAAGGATAAACGCAAAAAAGCCTTTCATGTCGGCGGTGCCTAAGcct < 2:382808/251‑2 (MQ=255) gAATACGGATGGCGTTAGAGATATGACCTTTATGTGCGCGTACCGGTTGTAGTGACGTCGGTTCGCCAATGATGGCGCAATCCGGGCGCAGAGCGGTAGTTTCGGCAAAATAACGCGCTCCGGCCA‑TACTGGTTTCTTCATCAGCAGTCGCCAGAATGTAGAGCGGTTTTTTCAGTTTCGTGACGTCGACATCGCGTAGCGCATCAAGGATAAACGCAAAAAAGCCTTTCATGTCGGCGGTGCCTAAGCCg > 1:235887/1‑251 (MQ=255) gATGGCGTTAGAGATATGACCTTTATGTGCGCGTACCGGTTGTAGTGACGTCGGTTCGCCAATGATGGCGCAATCCGGGCGCAGAGCGGTAGTTTCGGCAAAATAACGCGCTCCGGCCA‑TACTGGTTTCTTCATCAGCAGTCGCCAGAATGTAGAGCGGTTTTTTCAGTTTCGTGACGTAGACATCGCGTAGCGCATCAAGGATAAACGCAAAAAAGCCTTTCATGTCGGCGGTGCCTAAGCCGTAAAGCt < 1:383153/251‑1 (MQ=255) gCGTTAGAGATATGACCTTTATGTGCTCGTACCGGTTGTAGTGACGTCGGTTCGCCAATGATGGCGCAATCCGGGCGCAGAGCGGTAGTTTCGGCAAAATAACGCGCTCCGGCCA‑TACTGGTTTCTTCATCAGCAGTCGCCAGAATGTAGAGCGGTTTTTTCAGTTTCGTGACGTCGACATCGCGTAGCGCATCAAGGATAAACGCAAAAAAGCCTTTCATGTCGGCGGTGCCTAAGCCGTAAAGCTTGcc < 1:17912/251‑1 (MQ=255) gTTAGAGATATGTCCTTTATGTGCGCGTACCGGTTGTAGTGACGTCGGTTCGCCAATGATGGCGCAATCCGGGCGCAGAGCGGTAGTTTCGGCAAAATAACGCGCTCCGGCCA‑TACTGGTTTCTTCATCAGCAGTCGCCAGAATGTAGAGCGGTTTTTTCAGTTTCGTGACGTCGACATCGCGTAGCGCATCAAGGATAAACGCAAAAAAGCCTTTCATGTCGGCGGTGCCTAAGCCGTAAAGCTTGCCGt < 1:234973/251‑1 (MQ=255) agagATATGACCTTTATGTGCGCGTACCGGTTGGAGTGACGTCGGTTCGCCAATGATGGCGCAATCCGGGCGCAGAGCGGTAGTTTCGGCAAAATAACGCGCTCCGGCCA‑TACTGGTTTCTTCATCAGCAGTCGCCAGAATGTAGAGCGGTTTTTTCAGTTTCGTGACGTCGACATCGCGTAGCGCATCAAGGATAAACGCAAAAAAGCCTTTCATGTCGGCGGTGCCTAAGCCGTAAAGCTTGCCGTCa < 1:35110/250‑1 (MQ=255) gagaTATGACCTTTATGGGCGCGTACCGGTTGTAGGGACGTCGGTTCGCCAATGATGGCGCAATCCGGGCGCAGAGCGGTAGTTTCGGCAAAATAACGCGCTCCGGCCA‑TACTGGTTTCTTCATCAGCAGTCGCCAGAATGTAGAGCGGTTTTTTCAGTTTCGTGACGTCGACATCGCGTAGCGCATCAAGGATAAACGCAAAAAAGCCTTTCATGTCGGCGGTGCCTAAGCCGTAAAGCTTGCCGTCATg < 2:433309/251‑1 (MQ=255) gaTATGACCTTTATGTGCGCGTACCGGTTGTAGTGACGTCGGTTCGCCAATGATGGCGCAATCCGGGCGCAGAGCGGTAGTTTCGGCAAAATAACGCGCTCCGGCCA‑TACTGGTTTCTTCATCAGCAGTCGCCAGAATGTAGAGCGGTTTTTTCAGTTTCGTGACGTCGACATCGCGTAGCGCATCAAGGATAACCGCAAAAAAGCCTTTCATGTCGGCGGAGCCTAAGCCGTAAAGCTTGCCGTCATGCt > 1:209638/1‑251 (MQ=255) ccTTTATGTGCGCGTCCCGGTTGTAGTGACGTCGGTTCGCCAATGATGGCGCAATCCGGGCGCAGAGCGGTAGTTTCGGCAAAATAACGCGCTCCGGCCA‑TACTGGTTTCTTCATCAGCAGTCGCCAGAATGTAGAGCGGTTTTTTCAGTTTCGTGACGTCGACATCGCGTAGCGCATCAAGGATAAACGCAAAAAAGCCTTTCATGTCGGCGGTGCCTAAGCCGTAACGCTTGCCGGCATGCTCCGTc > 1:358042/1‑249 (MQ=255) tACCGGTTGTAGTGACGTCGGTTCGCCAATGATGGCGCAATCCGGGCGCAGAGCGGTAGTTTCGGCAAAATAACGCGCTCCGGCCA‑TACTGGTTTCTTCATCAGCAGTCGCCAGAATGTAGAGCGGTTTTTTCAGTTTCGTGACGTCGACATCGCGTAGCGCATCAAGGATAAACGCAAAAAAGCCTTTCATGTCGGCGGTGCCTAAGCCCGAAAGCTTGCCGGCCTGCCCCGTCCGTGTAAACGGactgc > 2:166555/1‑247 (MQ=255) aCCGGTTGTAGTGACGTCGGTTCGCCAATGATGGCGCAATCCGGGCGCAGAGCGGTAGTTTCGGCAAAATAACGCGCTCCGGCCA‑TACTGGTTTCTTCATCAGCAGTCGCCAGAATGTAGAGCGGTTTTTTCAGTTTCGTGACGTCGACATCGCGTAGCGCATCAAGGATAAACGCAAAAAAGACTTTAATGACGGCGGTGCCTAAGCCGGAAACCTTTCCGGCCTGCCCCGCCCGGGTAAACGGactacc > 2:400586/1‑246 (MQ=255) gtagtgtaggcGGTTCGCCAATGATGGCGCAATCCGGGCGCAGCGCGGTAGTTTCGGCAAAATAACGCGCTCCGGCCA‑TACTGGTTTCTTCATCAGCAGTCGCCAGAATGTAGAGCGGTTTTTTCAGTTTCGTGACGTCGACATCGCGTAGCGCATCAAGGATAAACGCAAAAAAGCCTTTCATGTCGGCGGTGCCTAAGCCGTAAAGCTTGCCGTCATGCTCCGTCAGTGTAAACGGATCGCGCGTCCa < 2:438356/240‑1 (MQ=255) aGTGACGTCGGTTCGCCAATGATGGCGCAATCCGGGCGCAGAGCGGTAGTTTCGGCAAAATAACGCGCTCCGGCCA‑TACTGGTTTCTTCATCAGCAGTCGCCAGAATGTAGAGCGGTTTTTTCAGTTTCGTGACGTCGACATCGCGTAGCGCATCAAGGATAAACGCAAAAAAGCCTTTCATGTCGGCGGTGCCTAAGCCGTAAAGCTTGCCGTCATGCTCCGTCAGTGTAAACGGATCGCGCGTCCAGc < 1:49679/250‑1 (MQ=255) tCGCCAAGGAGGGCGCAATCCGGGCGCAGAGCGGTAGTTTCGGCAAAATAACGCGCTCCGGCCA‑TACTGGTTTCTTCATCAGCAGTCGCCAGAATGTAGAGCGGTTTTTTCAGTTTCGTGACGTCGACATCGCGTAGCGCATCAAGGATAAACGCAAAAAAGCCTTTCATGTCGGCGGTGCCTAAGCCGTAAAGCTTGCCGTCATGCTCCGTCAGTGTAAACGGATCGCGCGTCCAGCGACCGTCATCaa < 2:99505/250‑1 (MQ=255) ccAATGATGGCGCAATCCGGGCGCAGAGCGGTAGTTTCGGCAAAATAACGCGCTCCGGCCA‑TACTGGTTTCTTCATCAGCAGTCGCCAGAATGTAGAGCGGTTTTTTCAGTTTCGTGACGTCGACATCGCGTAGCGCATCAAGGATAAACGCAAAAAAGCCTTTCATGTCGGCGGTGCCTAAGCCGTAAAGCTTGCCGTCATGCTCCGTCAGTGTAAACGGGTCGCGCGTCCAGCGACCGGCATCCAATgg > 2:89355/1‑251 (MQ=255) gggCGCAGAGCGGTAGTTTCGGCAAAATAACGCGCTCCGGCCA‑TACTGGTTTCTTCATCAGCAGTCGCCAGAATGTAGAGCGGTTTTTTCAGTTTCGTGACGTCGACATCGCGTAGCGCATCAAGGATAAACGCAAAAAAGCCTTTCATGTCGGCGGTGCCTAAGCCGTAAAGCTTGCCGTCATGCTCCGTCAGTGTAAACGGATCGCGCGTCCAGCGACCGTCATCAAATGGCACCg < 2:309199/238‑1 (MQ=255) gggCGCAGAGCGGTAGTTTCGGCAAAATAACGCGCTCCGGCCA‑TACTGGTTTCTTCATCAGCAGTCGCCAGAATGTAGAGCGGTTTTTTCAGTTTCGTGACGTCGACATCGCGTAGCGCATCAAGGATAAACGCAAAAAAGCCTTTCATGTCGGCGGTGCCTAAGCCGTAAAGCTTGCCGTCATGCTCCGTCAGTGTAAACGGATCGCGCGTCCAGCGACCGTCATCAAATGGCACCg > 1:309199/1‑238 (MQ=255) gcggggCGGTAGTTTCGGCAAAATAAGGCGCTCCGGCCA‑ATCTGGTATCTTCATCAGCAGTTGCCAGAATGTAGGGCGGTTTTTTCAGTTTCGTGACGTCGACATCGCGTAGCGCATCAAGGATAATCGCAAAAAAGCCTTTCATGTCGGCGGTGCCTAAGCCGTAAAGCTTGCCGTCAAGCTCCGTCAGTGTAAACGGTTCGCGCGACCAGCGACCGTCATCAAATGGCGCCGCATCGGTATGCCCCGcc < 2:132059/248‑1 (MQ=255) | AGGTTGAGCGTAGGGTATGGCACGGTAAACGCTTCGTAGTGATAACGTTCTTTCAGGTTATCGCGCAATTGCAAAATATGCCCGATGGCGTCGTGCATTAGTTCGATAGCGTTAACTCCGCGTGCTGGATCGCTGGAGTGCCCCGACTGGCCCTGAATACGGATGGCGTTAGAGATATGACCTTTATGTGCGCGTACCGGTTGTAGTGACGTCGGTTCGCCAATGATGGCGCAATCCGGGCGCAGGGCGGTAGTTTCGGCAAAATAACGCGCTCCGGCCA‑TACTGGTTTCTTCATCAGCAGTCGCCAGAATGTAGAGCGGTTTTTTCAGTTTCGTGACGTCGACATCGCGTAGCGCATCAAGGATAAACGCAAAAAAGCCTTTCATGTCGGCGGTGCCTAAGCCGTAAAGCTTGCCGTCATGCTCCGTCAGTGTAAACGGATCGCGCGTCCAGCGACCGTCATCAAATGGCACCGTATCGGTATGCCCCGCC > NC_000913/4154126‑4154617 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |