Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	65,905	C→T	D216N (GAT→AAT)	araD ←	L‑ribulose‑5‑phosphate 4‑epimerase AraD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	65,905	0	C	T	97.5%	116.5 / ‑4.0	40	D216N (GAT→AAT)	araD	L‑ribulose‑5‑phosphate 4‑epimerase AraD
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base T (20/19);  total (21/19)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.15e-01

ACTCTTGCGGTGCAAGCGTAACCTGCAACGGCCCGTTGTCCGTCGCCAGCCAGAAGGAGACTTCTGTCCCTTGCGGGGTGTCCCGCCAGTGTCGGGTTAAGATAAAACCTGCCTGCGCCACGCTGAAAATCCATCAAAAAACCAGGCTTGAGTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCGCCATGCTTACGCAGATAGTGTTTATCCAGCAGCGTTTGCTGCATATCCGGTAACTGCGGCGCTAACTGACGGCAGAATATCCCCATATAAGCGACCTCTTCCAGCACGATGGCGTTATGCACCGCATCTTCGGCATTTTTGCCCCATGCAAACGGGCCGTGGGAATGGACCAGAACGCCGGGCATTTGCGCTGCATCGATACCCTGTTTTTCAAAGGTTTCTACGATGACGTTACCGGTTTCCCACTCATATTCGCCGTTGATTTCTGCG  >  NC_000913/65660‑66149                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                     aCTCTTGCGGTGCAAGCGTAACCTGCAACGGCCCGTTGTCCGTCGCCAGCCAGAAGGAGACTTCTGTCCCTTGCGGGGTGTCCCGCCAGTGTCGGGTTAAGATAAAACCTGCCTGCGCCACGCTGTAATTCCATCAAAAAACCAGGCTTGATTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCGCCATGCTTACGCAGATAGGGTTTATTcagc                                                                                                                                                                                                                                                  >  1:415002/1‑250 (MQ=255)       gCGGTGCAAGCGTAACCGTCAACGCCCCGTTGTCCGTCGCCAGCCATAAGGAGCCTTCTGTCCCTTGCGGGGTTTCACGTCAGTGTCGGGTTAAGATAAAACCTGCCTGCGCCACGCTGTAATTCCATCAAAAAACCAGGCTTGATTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACATTCATTACTGCCCGTAATATGCCTTCGCGCCATGCTTACGCAGATAGTGTTTATTCAGCAGCGttt                                                                                                                                                                                                                                           <  1:276648/251‑1 (MQ=255)                         gCAACGGCCCGTGGTCCGTCGACAGCCAGAAGGAGCTTTCTGTCCCTTGCGGGGTGTTCCGCCAGTGTCGGGTTAAGATAAAACCTGCCTGCGCCACGCTGTAATTCCAACAAAAATCCAGGCTTGATTATAGCCTGGTGTCGTTTGATTGGCTGTGGTTTTATACAGGCATTACTGCCCGTAATATGTCTTCGCGCCATGCTTACGCAGATAGTGTTTATTCAGCAGCGTTTGCTGCATATCCGGt                                                                                                                                                                                                                             <  1:282102/247‑1 (MQ=255)                                  cGTTGTCCGTCGCCAGCCAGAAGGAGACTTCTGTCCCTTGCGGGGTGTCCCGCCAGTGTCGGGTTAAGATAAAACCTGCCTGCGCCACGCTGTAATTCCATCAAAAAACCAGGCTTGATTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCCCGCCATGCTTACGCAGGTAGTTTTTTTTTAGCAGCGTTTGCTTCCTATCCGGTACATGCGGCGCt                                                                                                                                                                                                                 >  2:12742/1‑250 (MQ=255)                                   gTTGTCCGCCGCCAGCCAGAAGGAGACTTCTGTCCCTTGCGGGGTGTCCCGCCAGTGTCGGGTTAAGATAAAACCTGCCTGCGCCACGCTGTAATTCCATCAAAAAACCAGGCTTGATTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCGCCATGCTTACGCAGATAGTGTTTATTCAGCAGCGTTTGCTGCATATCCGGTAACTGCGGCGCTaa                                                                                                                                                                                                               <  1:460820/251‑1 (MQ=255)                                        cggTCGCCAGCAAGAAGGAGACTTCTGCCCCTTGCGGGGTGTTCCGCCAGTGTCGGGTAAAGATAAAACCTGCCTGCGCCACGCTGTAATTCCATCACAAAACCAGGCTTGATTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCGCCATGCTTACGCAGATAGTGTTTATTCAGCAGCGTTTGCTGCATATCCGGTAACTGCGGCGCTAACTGAc                                                                                                                                                                                                          <  1:415011/249‑1 (MQ=255)                                                          agaCTTCTGTCCCTTGCGGGGTGTCCCGCCAGTGTCGGGGTAAGATTAAACCTGCCTGCGCCCCGCCGTAATTCCATCAAAAAACCAGGCTTGATTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCCTAATATGCCTTCGCGCCATGCTTACGCCGATAGGGTTTTTTCAGCAGCGTTTGCTTGATATCCGGGAAATGCGGGGCTAACTTACGGCAGAATACCCCCatct                                                                                                                                                                                        >  2:47674/1‑249 (MQ=255)                                                                    cccTTGCGGGGTGTCCCGCCAGTGTCGGGTTAAGATAAAACCTGCCTGCGCCACGCTGTAATTCCATCAAAAAACCAGGCTTGATTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCGCCATGCTTACGCAGATAGTGTTTATTCAGCAGCGTTTGCTGCATATCCGGTAACTGCGGCGCTAACTGACGGCAGAATATCCCCATATAAGCGACctc                                                                                                                                                                              <  1:354308/251‑1 (MQ=255)                                                                           agggTGTCCCGCCAGTGTCGGGTTAAAATAAAACCTGCCTGCGCCACGCTGTAATTCCATCAAAAAACCAGGCTTGATTATAGCCTGGTTTCGGTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATTCCTTCGCGCCATTCTTACGCAGAGTGTGTTTTTTTCGAAGCGTTTGATGCCTATCCGGTAACTTGGGCGCTAACCGACGGCAGAATATTCCCATATaaacctcctcctcccgc                                                                                                                                                                       >  2:379824/2‑236 (MQ=255)                                                                                    cGCCAGTGTCGGGTTAAAATAAAACCTGCCTGCGCCACGCTGTAATTCCATCCAAAAACCAGGCTTGATTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCCTCGCGCCATGCTTACGCCGATAGTGTTTATTCAGCCGCGTTTGCTGCATATCCGGTAACTTCGGGGCTAACTTGCGGCCGAATTTACCCATATAAGCGACCTCTTCCCGCACGATGGCg                                                                                                                                                              >  2:128284/1‑251 (MQ=255)                                                                                        aGTGTCGGGTTAAGATAAAACCTGCCTGCGCCACGCTGTAATTCCATCAAAAAACCAGGCTTGATTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCGCCATGCTTACGCAGATAGTGTTTATTCAGCAGCGTTTGCTGCATATCCGGTAACTGCGGCGCTAACTGACGGCAGAATATCCCCATATAAGCGACCTCTTCCAGCACGATGGCGTTAt                                                                                                                                                          <  1:23030/251‑1 (MQ=255)                                                                                           gtCGGTTTAAGATAAAACCTGCCTGCGCCACGCTGTAATTCCATCAAAAAACCAGGCTTGATTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCGCCATGCTTACGCAGATAGTGTTTATTCAGCAGCGTTTGCTGCATATCCGGTAACTGCGGCGCTAACTGACGGCAGAATATCCCCATATAAGCGACCTCTTCCAGCACGATGGCGTTATGCa                                                                                                                                                       <  2:378128/251‑1 (MQ=255)                                                                                                   aaGAGAAAACCTGCCTGCGCAACGCTGTAATTCCATCAAAAAACCAGTCTTGATTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCTTTACTGCCCGTAATATGCCTTCGCGCCATGTTTACTCAGATAGTGTTTATTCAGCATCGTGTGCTGCATATCCGGTAACTGCGGCGCTAACTGACGGCAGAATAGCCCCATATAATCGACCTCTTCCAGCACGATGGCGTTATGCTCCGCagct                                                                                                                                               <  1:425539/251‑4 (MQ=255)                                                                                                          aaCCGGCCTGCGCCACGCTGTAATTCCATCAAAAAACCAGGCTTGATTATAGCCTTGTGTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCGTCGCGCCATGCTTACGCAGATAGTGTTTATTCATCAGCGTTTGCTGCATATCCGGTAACTGCGGCGCTAACTGACGGCAGAATATCCCCATATAAGCGACCTCTTCCAGCACGATGGCGTTATGCACCGCATCTTCGGCAt                                                                                                                                        <  1:433134/251‑1 (MQ=255)                                                                                                               gcctgcGCCACGCTGTAATTCCATCAAAAAACCAGGCTTGATTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCGCCATGCTTACGCAGATAGTGTTTATTCAGCAGCGTTTGCTGCATATCCGGTAACTGCGGCGCTAACTGACGGCAGAATATCCCCATATAAGCGACCTCTTCCAGCACGATGGCGTTATGCACCGCATCTTCGGCAtttt                                                                                                                                     <  1:12953/249‑1 (MQ=255)                                                                                                                      ccACGCTGTAAATCCAGCAAAAAACCAGGCTTGATTTTAGCCTGGTTTCGTTTGATTGGCGGTGGTTTTATACAGTCATTACTTCCCGTAATATGCCTTCGCGCCATGCTTACGCAGATAGTGTTTATTCAGCAGCGTTTGCTGCATATCCGGTAACTGCGGCGCTAACTGACGGCAGAATATCCCCATATAAGCGTCCTCTTCCAGCACGATGGCGTTATGCACCGCATCTTCGTCATTTTTGcccatgc                                                                                                                            <  2:83784/251‑5 (MQ=255)                                                                                                                          gctgtaattCCATCAAAAAACCAGGCTTGATTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCGCCATGCTTACGCAGATAGTGTTTATTCAGCAGCGTTTGCTGCATATCCGGTAACTGCGGCGCTAACTGACGGCAGAATATCCCCATATAAGCGACCTCTTCCAGCACGATGGCGTTATGCACCGCATCTTCGGCATTTTTGCCC‑ATGCaaat                                                                                                                       <  1:163546/243‑2 (MQ=255)                                                                                                                                                        ttataGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCGCCATGCTTACGCAGATAGTGTTTATTCAGCAGCGTTTGCTGCATATCCGGTAACTGCGGCGCTAACTGACGGCAGAATATCCCCATATAAGCGACCTCTTCCAGCACGATGGCGTTATGCACCGCATCTTCGGCATTTTTGCCC‑ATGCCAATGGGCCGTGCGAATGGACCAGGACGCCGGGc                                                                                         >  1:426025/2‑251 (MQ=255)                                                                                                                                                                       gTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCGCCATGCTTACGCAGATAGAGTTTATTCAGCAGCGTTTGCTGCATATCCGGTAACTGCGGCGCTAACAGCCGGCAGATTATCCCCATATAAGCGACCTCTTCCAGCACGATGGCGTTATGCACCGCATCTTCGGCATTTTTGCCC‑ATGCAAATGGGCCGTGAGAATGGACCAGGACGCCGGGCATTTGCGCTGCATCg                                                                          >  1:341356/1‑251 (MQ=255)                                                                                                                                                                               ggCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCGCCATGCTTACGCAGATAGTGTTTATTCAGCAGCGTTTGCTGCATATCCGGTAACTGCGGCGCTAACTGACGGCAGAATATCCCCATATAAGCGACCTCTTCCAGCACGATGGCGTTATGCACCGCATCTTCGGCATTTTTGCCC‑ATGCAAATGGGCCGTGAGAATGGACCAGGACGCCGGGCATTTGCGCTGCATCGATACCCTg                                                                  >  2:356135/1‑251 (MQ=255)                                                                                                                                                                                gCTGTGGTTTTATACAGTCATTCCTGCACGTAATCTGCCTTCGCGCCATGCTTACGCAGATAGTGTTTATTCAGCAGCGTTTGCTGCATATCCGGTAACTGCGGCGCTAACTGACGGCAGAATATCCCCATATAAGCGACCTCTTCCAGCACGATGGCGTTATGCACCGCATCTTCGGCATTTTTGCCC‑ATGCAAATGGGCCGTGAGAATGGACCAGGCCGCCGGGCATTTGCGCTGCATCGATACCCTGt                                                                 >  2:64720/1‑251 (MQ=255)                                                                                                                                                                                gCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCGCCATGCTTACGCAGATAGTGTTTATTCAGCAGCGTTTGCTGCATATCCGGTAACTGCGGCGCTAACTGACGGCAGAATATCCCCATATAAGCGACCTCTTCCAGCACGATGGCGTTATGCACCGCATCTTCGGCATTTTTGCCC‑ATGCAAATGGGCCGTGAGAATCGACCAGGACGCCGGGCATTTGCGCTGCATCGATACCCTGt                                                                 >  2:206653/1‑251 (MQ=255)                                                                                                                                                                                gCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCGCCATGCTTACGCAGATAGTGTTTATCCAGCAGCGTTTGCTGCATATCCGGTAACTGCGGCGCTAACTGACGGCAGAATATCCCCATATAAGCGACCTCTTCCAGCACGATGGCGTTATGCACCGCATCTTCGGCATTTTTGCCCCATGCAAACGGGCCGTGGGAATGGACCAGAACGCCGGGCATTTGCGCTGCCTCGATACCCTg                                                                  >  2:139186/1‑251 (MQ=255)                                                                                                                                                                                           ataCAGTCATTACTGCCCGTAATATGCCTTCGCGCCATGCTTACGCAGATAGTGTTTATTCAGCAGCGTTTGCTGCATATCCGGTAACTGCGGCGCTAACTGACGGCAGAATATCCCCATATAAGCGACCTCTTCCAGCACGATGGCGTTATGCACCGCATCTTCGGCATTTTTGCCC‑ATGCAAATGGGCCGTGAGAATGGACCAGGACGCCGGGCATTTGCGCTGCATCGATACCCTGTTTTTCGAAGGt                                                      <  1:356135/251‑1 (MQ=255)                                                                                                                                                                                               aGTCATTACTGCCCGTAATATGCCTTCGCGCCATGCTTACGCAGATAGTGTTTATTCAGCAGCGTTTGCTGCATATCCGGTAACTGCGGCGCTAACTGACGGCAGAATATCCCCATATAAGCGACCTCTTCCAGCACGATGGCGTTATGCACCGCATCTTCGGCATTTTTGCCC‑ATGCAAATGGGCCGTGAGAATGGACCAGGACGCCGGGCATTTGCGCTGCATCGATACCCTGTTTTTCGAAGGTTTCt                                                  <  1:12742/251‑1 (MQ=255)                                                                                                                                                                                                         gCCCGTAATATGCCTTCGCGCCATGCTTACGCAGCTAGTGTTTATTCAGCAGCGTTTGCTGCATATCCGGTAACTGCGGCGCTAACTGACGGCAGAATATCCCCATATAAGCGACCTCTTCCAGCCCGATGGCGTTATGCACCGCATCTTCGGCATTTTTGCCC‑ATGCAAATGGGCCGTGAGAATGGACCAGGACGCCGGGCATTTGCGCTGCATCGATACCCTGTTTTTCGAAGGTTTCTACGATGACGt                                        >  2:438626/1‑251 (MQ=255)                                                                                                                                                                                                             gTAATATGCCTTCGCGCCATGCTTACGCAGATAGTGTTTATTCAGCAGCGTTTGCTGCATATCCGGTAACTGCGGCGCTAACTGACGGCAGAATATCCCCATATAAGCGACCTCTTCCAGCACGATGGCGTTATGCACCGCATCTTCGGCATTTTTGCCC‑ATGCAAATGGGCCGTGAGAATGGACCAGGACGCCGGGCATTTGCGCTGCATCGATACCCTGTTTTTCGAAGGTTTCTACGATGACGTTAcc                                    >  1:310626/1‑251 (MQ=255)                                                                                                                                                                                                                atatGCCTTCGCGCCATGCTTACGCAGATAGAGTTTATTCAGCAGCGTTTGCTGCATATCCGGTAACTGCGGCGCTAACTGACGGCAGAATATCCCCATATAAGCGACCTCTTCCAGCACGATGGCGTTATGCACCGCATCTTCGGCATTTTTGCCC‑ATGCAAATGGGCCGTGAGAATGGACCAGGACGCCGGGCATTTGCGCTGCATAGATACCCTGTTTTTCGAAGGTTTCTACGATGACGTTACCGGt                                 >  1:412876/1‑251 (MQ=255)                                                                                                                                                                                                                            gcaaTGCTTACGCAGATAGTGTTGATTCAGCAGCGTTTGCTGCATATCCGGTAACTGCGGCGCTAACTGACGGCAGAATATCCCCATATAAGCGACCTCTTCCAGCACGATGGCGTTATGCACCGCATCTTCGGCATTTTTGCCC‑ATGCAAATGGGCCGTGAGAATGGACCAGGACGCCGGGCATTTGAGCTGCATCGATACCCTGTTTTTCGAAGGTTTCTACGATGACGTTACCGGTTTCCCACTCata                     <  1:150033/248‑1 (MQ=255)                                                                                                                                                                                                                            gcCATGCTTACGCAGATAGTGTTTATTCAGCAGCGTTTGCTGCATATCCGGTAACTGCGGCGCTAACTGACGGCAGAATATCCCCATATAAGCGACCTCTTCCAGCACGATGGCGTTATGCACCGCATCTTCGGCATTTTTGCCC‑ATGCAAATGGGCCGTGAGAATGGACCAGGACGCCGGGCATTTGCGCTGCATCGATACCCTGTTTTTCGAAGGTTTCTACGATGACGTTACCGGTTTCCCACTCata                     <  1:64720/251‑1 (MQ=255)                                                                                                                                                                                                                                tGCTTACGCAGATAGTGTTTATTCAGCAGCGTTTGCTGCATATCCGGTAACTGCGGCGCTAACTGACGGCAGAATATCCCCATATAAGCGACCTCTTCCAGCACGATGGCGTTATGCACCGCATCTTCGGCATTTTTGCCC‑ATGCAAATGGGCCGTGAGAATGGACCAGGACGCCGGGCATTTGCGCTGCATCGATACCCTGTTTTTCGAAGGTTTCTACGATGACGTTACCGGTTTCCCACTCATATTc                  >  1:199514/1‑250 (MQ=255)                                                                                                                                                                                                                                 gCTTACGCAGATAGTGTTTATTCAGCAGCGTTTGCTGCATATCCGGTAACTGCGGCGCTAACTGACGGCAGAATATCCCCATATAAGCGACCTCTTCCAGCACGATGGCGTTATGCACCGCATCTTCGGCATTTTTGCCC‑ATGCAAATGGGCCGTGAGAATGGACCAGGACGCCGGGCATTTGCGCTGCATCGATACCCTGTTTTTCGAAGGTTTCTACGATGACGTTACCGGTTTCCCACTCATATTca                 <  1:5153/250‑2 (MQ=255)                                                                                                                                                                                                                                 gCTTACGCAGATAGTGTTTATTCAGCAGCGTTTGCTGAATATCCGTAAACTGCGGCGCTAACTGACGGCAGAATACCCCCATATAAGCGACCTCTTCCAGCACGATGGCGTTATGCACCGCATCTGCGGCATTTTTGCCC‑ATGCAAATGGGCCGTTAGAATGGACCAGGACGCCGGGCATTTGCGCTGCATCGATACCCTGTTTTTCGAAGGTTTCTACGATGACGTTACCGGTTTCCCACTCATATTca                 <  2:358278/250‑2 (MQ=255)                                                                                                                                                                                                                                  cTTACGCAGATAGTGTTTATTCAGCAGCGTTTGCTGCATATCCGGTAACTGCGGCGCTAACTGACGGCAGAATATCCCCATATAAGCGACCTCTTCCAGCACGATGGCGTTATGCACCGCATCTTCGGCATTTTTGCCC‑ATGCAAATGGGCCGTGAGAATGGACCAGGACGCCGGGCATTTGCGCTGCATCGATACCCTGTTTTTCGAAGGTTTCTACGATGACGTTACCGGTTTCCCACTCATATTcacc               >  1:235404/1‑248 (MQ=255)                                                                                                                                                                                                                                   ttACGCAGATAGTGTTTATTCAGCAGCGTTTGCTGCATATCCGGTAACTGCGGCGCTAACTGACGGCAGAATATCCCCATATAAGCGACCTCTTCCAGCACGATGGCGTTATGCACCGCATCTTCGGCATTTTTGCCC‑ATGCAAATGGGCCGTGAGAATGGACCAGGACGCCGGGCATTTGCGCTGCATCGATACCCTGTTTTTCGAAGGTTTCTACGATGACGTTACCGGTTTCCCACTCATATTCACCg              >  2:38959/1‑251 (MQ=255)                                                                                                                                                                                                                                     aCGCAGATAGTGTTTATTCAGCAGCGTTTGCTGCAGAACCGGTAACTGCGGCGCTAACTGACGGCAGAATATCCCCATATAAGAGACCTCTTCCAGCACGATGGCGTTATGCACCGAATCTTCGGCATTTTTGCCC‑ATGCAAATGGGCCGTGAGAATGGACCAGGACGCCGGGCATTTGCGCTGCATCGATACCCTGTTTTTAGAAGGTTTCTACGATGACGTTACCGGTTTCCCGCTCATATTcacagtt            >  2:41979/1‑245 (MQ=255)                                                                                                                                                                                                                                         aGATAGTGTTTATTCAGCAGCGTTTGCTGCATATCCGGTAACTGCGGCGCTAACTGACGGCAGAATATCCCCATATAAGCGACCTCTTCCAGCACGATGGCGTTATGCACCGCATCTTCGGCATTTTTGCCC‑ATGCAAATGGGCCGTGAGAATGGACCAGGACGCCGGGCATTTGCGCTGCATCGATACCCTGTTTTTCGAAGGTTTCTACGATGACGTTACCGGTTTCCCACTCATATTCACCGTTGAtt        <  1:428399/251‑1 (MQ=255)                                                                                                                                                                                                                                              gtgtTTATTCAGCAGCGTTTGCTGCATATCCGGTAACTGCGGCGCTAACTGACGGCAGAATATCCCCATATAAGCGACCTCTTCCAGCACGATGGCGTTATGCACCGCATCTTCGGCATTTTTGCCC‑ATGCAAATGGGCCGTGAGAATGGACCAGGACGCCGGGCATTTGCGCTGCCTCGATACCCTGTTTTTCGAAGGTTTCTACGATGACGTTACCGGTTTCCCACTCATATTCACCGTTGATTTCTGc   >  2:124492/1‑251 (MQ=255)                                                                                                                                                                                                                                               tgtTTATTCAGCAGCGTTTGCTGCATATCCGGTAACTGCGGCGCTAACTGACGGCAGAATATCCCCATATAAGCGACCTCTTCCAGCACGATGGCGTTATGCACCGCATCTTCGGCATTTTTGCCC‑ATGCAAATGGGCCGTGAGAATGGACCAGGACGCCGGGCATTTGCGCTGCATCGTTACCCTGTTTTTCGCAGGTTTCTACGATTGCGTTACCGGTTTCCCACTCCTATTCACCTTTGATTTCTGcc  >  2:412342/1‑250 (MQ=255)                                                                                                                                                                                                                                               tgtTTATTCAGCAGCGTTTGCTGCATATCCGGTAACTGCGGCGCTAACTGACGGCAGAATATCCCCATATAAGCGACCTCTTCCAGCACGATGGCGTTATGCACCGCATCTTCGGCATTTTTGCCC‑ATGCAAATGGGCAGTGAGAATGGACCAGGACGCCGGGCATTTGCGCTGCATCGATACCCTGTTTTTCGAAGGTTTCTACGATGACGTTACCGGTTTCCCACTCATATTCACCGTTGATTTCTGCg  >  1:129173/1‑251 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                     ACTCTTGCGGTGCAAGCGTAACCTGCAACGGCCCGTTGTCCGTCGCCAGCCAGAAGGAGACTTCTGTCCCTTGCGGGGTGTCCCGCCAGTGTCGGGTTAAGATAAAACCTGCCTGCGCCACGCTGAAAATCCATCAAAAAACCAGGCTTGAGTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCGCCATGCTTACGCAGATAGTGTTTATCCAGCAGCGTTTGCTGCATATCCGGTAACTGCGGCGCTAACTGACGGCAGAATATCCCCATATAAGCGACCTCTTCCAGCACGATGGCGTTATGCACCGCATCTTCGGCATTTTTGCCCCATGCAAACGGGCCGTGGGAATGGACCAGAACGCCGGGCATTTGCGCTGCATCGATACCCTGTTTTTCAAAGGTTTCTACGATGACGTTACCGGTTTCCCACTCATATTCGCCGTTGATTTCTGCG  >  NC_000913/65660‑66149

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑