Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	76,358	C→A	G314G (GGG→GGT)	sgrR ←	DNA‑binding transcriptional dual regulator SgrR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	76,358	0	C	A	97.8%	126.6 / NA	45	G314G (GGG→GGT)	sgrR	DNA‑binding transcriptional dual regulator SgrR
Reads supporting (aligned to +/- strand):  ref base C (0/1);  new base A (23/21);  total (23/22)
Fisher's exact test for biased strand distribution p-value = 4.89e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TCGCTCTTTATGGTGCGGGCATGGTGCCAACGGGGGAGCAGTCCATAAGCCGGGAACCACAGTTGCTGGTACTGTTCCTCAGCGAAATAGACCAGATTAGTTGGAGAAAGCACATAGCTTACCCAGTCCCTGACTTGCTGATTCGCCCCGCGATGGGTGCGGCTGTCGAACAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCGCCCTGTGGACCTTTTAGCATCAGCCCTCCGGCTGGCTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCCCGGTAACCGAAGAAGTCATCGAATGCCTGAATTTTCAGTTGATTGGTGCTGTTGCGAATCACCGCATACGGACCGGTGCCGATGGGATGGCTGGCAAAGTTACTGAGGGTTTCCCATTCGCGCGGCAGGATCATCGCCGGAACTTGCCCCAGCAGTAACGGTAACCAGCGGTCCGGTT  >  NC_000913/76113‑76599                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                  tccctcgttATGGTGCGGGCATGGTGCCAACGGGGGAGCAGTCCATAAGCCGGGAACCACAGTTGCTGGTACTGTTCCTCAGCGAAATAGACCAGATTAGTTGGAGAAAGCACATAGCTTACCCAGTCCCTGACTTGCTGATTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTc                                                                                                                                                                                                                                               <  2:223009/243‑1 (MQ=255)    ctctTTCTGGTGCGGGCATGGTGCCAACGGGGGAGCAGTCCATAAGCCGGGAACCACAGTTGCTGGTACTGTTCCTCAGCGAAATAGACCAGATTAGTTGGAGAAAGCACATAGCTTACCCAGTCCCTGACTTGCTGATTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTccagc                                                                                                                                                                                                                                           >  1:409853/1‑248 (MQ=255)       tttATGGTGCGGGCATGGTGCCAACGGGGGAGCAGTCCATAAGCCGGGAACCACAGGTGCTGGTACTGTTCCTCAGCGAAATAGACCAGATTAGTTTGAGAAAGCACATAGCTTACCCAGTCCCTGACTTGCTGATTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCCCCCTGTGGACCTTTTAGCATCAGACCTCCagctg                                                                                                                                                                                                                                         <  1:449290/250‑1 (MQ=255)                    cATGGTGCCAACGGGGGATCAGTCCAGAAGCCGGGAACCACAGTTGCTGGTACTGTTCCTCAGCGAAATAGACCAGAGTAGTGGGAGAAAGCACATAGCTTACCCAGTCCCTGACTGGCTGATTCGCCCCGCGAGGGGTGCGGCTGTCGAATATTAAATAGTATCAACCTTCCTCCAGGCGGCTTGCAATCTCTCTATCCTCACCCTGGGGACCTTGTAGCATCATACCTCCAGCTGTCTCGGCTGCAAtt                                                                                                                                                                                                                           <  2:443901/251‑1 (MQ=255)                            cAACGGGGGAGCAGTCCATAAGCCGGGAACCACAGTTGCTGGTACTGTTCCTCAGCGAAATAGACCAGATTAGTTGGAGAAAGCACATAGCTTACCCAGTCCCTGACTTGCTGATTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGGCTCGTCGGCAATTTCCGGCAg                                                                                                                                                                                                                   <  1:72597/251‑1 (MQ=255)                               cgtgggAGCAGTCCATAAGCCGGGAACCACAGTTGCTGGTACTGTTCCTCAGCGAAATAGACCAGATTAGTTGGAGAAAGCACATAGCTTACCCAGTCCCTGACTTGCTGCTTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGGCTCGTCGGCAATTTCCGGCAGAAc                                                                                                                                                                                                                >  1:138917/4‑251 (MQ=255)                                     aGCAGTCCATAAGCCGGGCACCACAGTTGCTGTGACTGTTCCTCAGCGAAATAGACCCGATTAAGTGGAGAATGCACATAGCTTACCCAGTCCCTGACTTGCTGATTCGCCCCGCGATGGGAGCGGCTGTCGCATAGTAAATAGTAGCACCCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCGTCACCCTGTTGACCTTTTAGCATCAGACCTCCAGCTGGCTCGTCAGCAATTTCCTGCAGTACCCAGAc                                                                                                                                                                                                          >  2:15395/1‑251 (MQ=255)                                                                       ccgtctcatCAGCGAAATATACCACATTTGTTGGAGAAAGCTCATTGCTTTCCCAGGCCCTGACTTTATGATTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCCGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGTCCTTTTAGCATCAGACCTCCAGCTGGCTCTTCTGAAATTTCCGTCAGAACCCGCACGTTATCTGCGTCGATTAATGCCCGGTAACCgaa                                                                                                                                                                         <  2:463043/242‑1 (MQ=255)                                                                          gTTCCTCAGCGAAATAGACAAGATTAGTTGGAGAAAGCACATAGCTTTCCCAGTCCCTGACTTGCAGATTCGCCCCGCGAGGGGTGCGGCTGTCGAAGAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTACAAGCTCTTTTTCCTCTCCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGGCTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAATTTCGACGATTAATGCCCGGTAACCgaagaa                                                                                                                                                                      <  2:292930/250‑1 (MQ=255)                                                                                cAGCGAAATAGACCAGATTAGTTGGAGAAAGCACATAGCTTACCCAGTCCCTGACTTGCTGATTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGGCTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCTATTAATGCCCGGTAACCGAAGAAGTCAt                                                                                                                                                                 <  1:334826/249‑1 (MQ=255)                                                                                    gAAATAGACCAGATTAGTTGGAGAAAGCACATAGCTTACCCAGTCCCTGACTTGCTGATTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGGCTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAAAGCCCGGTAACAGAAGAAGTCATCGAATg                                                                                                                                                           >  2:83186/1‑251 (MQ=255)                                                                                     aaaTAGACCAGATTAGTTGGAGAAAGCAAATAGCTTACCCAGTCCCTGACTTGCTGATTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTTAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTAGCTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGAGTAATGCCCGGTAAGCAaagaagcaaacgaatgc                                                                                                                                                          >  1:281737/1‑240 (MQ=255)                                                                                      aaTAGACCAGATTAGTTGGAGAAAGCACATAGCTTACCCAGTCCCTGACTTGCTGATTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGGCTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCCCGGTAACCGAAGAAGTCATCGAATGcc                                                                                                                                                         <  1:199667/251‑1 (MQ=255)                                                                                       aTAGACCAGATTAGTTGGAGAAAGCACATAGCTTACCCAGTCCCTGACTTGCTGATTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCCTCAGAACTCCAGCTGGCTCGTCGGCAATTTCCGGCAGAACCCAGCCGTTAACTTCGTCGGTTAATGCCCGGAAACCGAAGACGTCATCGAATGCCt                                                                                                                                                        >  2:274075/1‑251 (MQ=255)                                                                                       aTAGACCAGATTAGTTGGAGAAAGCACATAGCTTACCCAGTCCCTGACTTGCTGATTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGGCTAGTCGGCACTTTCCGGCAGAACCCAGACGTTAACTTAGTCGATTAATGCCCGGTAACCGAAGAAGTCATCGAATGCCt                                                                                                                                                        >  1:209247/1‑251 (MQ=255)                                                                                                  tAGTTGGAGAAAGCACATAGCTTACCCAGTCCCTGACTTGCTGATTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGGCTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCCCGGTAACCGAAGAAGTCATCGAATGCCTGAATTTTCAGt                                                                                                                                             >  1:256690/1‑251 (MQ=255)                                                                                                   aGTTGGAGAAAGCCCATAGCTTACCCAGTCCCTGACTTGCTGAATCGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGGCTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCCCGGTAACCGAAGAAGGCATCGAATGCCTGAATTTTCAGt                                                                                                                                             <  2:171929/250‑1 (MQ=255)                                                                                                   aGTTGGAGAAAGCACATAGCTTACCCAGTCCCTGACTTGCTGATTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGGCTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCCCGGTAACCGAAGAAGTCATCGAATGCCTGAATTTTCAGtt                                                                                                                                            >  1:433052/1‑251 (MQ=255)                                                                                                                     gCTTACCCAGTCCCTGACTTGCTGATTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGGCTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGACCGGTAACCGAAGAAGTCATCGAATGCCTGAATTTTCAGTTGATTGGTGCTGTTGCgg                                                                                                                           >  2:42548/1‑249 (MQ=255)                                                                                                                          cccAGTCCCTGACTTGCTGATTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGGCTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCCCGGTAACCGAAGAAGTCATCGAATGCCTGAATTTTCAGTTGATTGGTGCTGTTGCGGATCAcc                                                                                                                     <  1:11980/251‑1 (MQ=255)                                                                                                                           ccAGTCCCTGACTTGCTGATTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGGCTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCCCGGTAACCGAAGAAGTCATCGAATGCCTGAATTTTCAGTTGATTGGTGCTGTTGCGGATCACCg                                                                                                                    >  1:8375/1‑251 (MQ=255)                                                                                                                             agacccTGACTTGCTGATTCGCCCCGCGATGGTTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCACTCTCTTTTTCCTCACCCTGTGGACCTTTTAGCCTCAGACCTCCAGCTGGCTCGTCGGCCATTTCCGACAGAACCCAGACGTTAACTTCGTCGTTTAATGCCCGGTCACCGAAAAAGTCCTCAAATGCCTGCATTTTCCGTTGATTGGTGAGGTTGCggtacacagcc                                                                                                                  >  2:377362/4‑241 (MQ=255)                                                                                                                              gTCCCTGACTTGCTGATTCGTCCCGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGGCTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCCCGGTAACCGAAGAAGTCATCGAATGCCTGAATTTGCAGTTGATTGGTGCTGTTGCGGATCACCGCAt                                                                                                                 <  2:246735/251‑1 (MQ=255)                                                                                                                              gTCCCTGACTTGCTGATTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGGCTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCCCGGTAACCGAAGAAGTCATCGAATGCCTGAATTTTCAGTTGATTGGTGCTGTTGCGGATCACCGCAt                                                                                                                 <  1:299426/251‑1 (MQ=255)                                                                                                                               tCCCTGACTTGCTGATTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGGCTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTa                                                                                                                                                                                         <  2:355615/178‑1 (MQ=255)                                                                                                                               tCCCTGACTTGCTGATTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGGCTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTa                                                                                                                                                                                         >  1:355615/1‑178 (MQ=255)                                                                                                                                   tGACTTGCTGATTCGCCCCGCGATGGGTGAGGCTGACGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCCTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGGCTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCCCGGTAACCGAAGAAGTCATCGAATGCCTGAATTTTCAGTTGATTGGTGCTGTTGCGGATCACCGCATACggg                                                                                                            >  1:339837/1‑250 (MQ=255)                                                                                                                                    gACTTGCTGATTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGGCTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCCCGGTAACCGAAGAAGTCATCGAATGCCTGAATTTTCAGTTGATTGGTGCTGTTGCGGATCACCGCATACGGAc                                                                                                           >  1:306916/1‑251 (MQ=255)                                                                                                                                             aTTCGCCCCGCGATGGGTGCGGCTGTCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGGCTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCCCGGTAACCGAAGAAGTCATCGAATGCCTGAATTTTCAGTTGATTGGTGCTGTTGCGGATCACCGCATACGGACCGGTGCCg                                                                                                   >  1:455905/1‑250 (MQ=255)                                                                                                                                                                      tCGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGGCTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCCCGGTAACCGAAGAAGTCATCGAATGCCTGAATTTTCAGTTGCTTGGTGCTGTTGCGGATCCCCGCATACGGACCGGTGCCGCTGGGATGGCTGGCAAAGTTACTg                                                                           >  2:293906/1‑249 (MQ=255)                                                                                                                                                                       cGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCCCCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGGCTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCCCGGTAACCGAAGAAGTCCTCGAATGCCTGAATTTTCAGTTGATTGGTGCTGTAGCGGATCACCGACTACGGACCGGTGCCGATGGGATGGCTGGCAAAGTTACTGa                                                                          >  2:312258/1‑249 (MQ=255)                                                                                                                                                                       cGAATAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGGCTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCCCGGTAACCGAAGAAGTCATCGAATGCCTGAATTTTCAGTTGATTGGTGCTGTTGCGGATCACCGCATACGGACCGGTGCCGATGGGATGGCTGGCAAAGTTACTGAg                                                                         <  1:289332/250‑1 (MQ=255)                                                                                                                                                                               aaaTAGTAGCATCCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCGCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGGCTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTCCGTCGATTAATGCCCGGTAACCGAAGAAGTCATTGAATGCCTGAATTTTCAGTTGATTGGTGCTGTTGCGGATCACCGCATACGGACCGGTGCCGATGGGATGGCTGGCAAAGTTACTGAGGGTTTccc                                                                 <  1:33916/250‑1 (MQ=255)                                                                                                                                                                                aaTAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTGTCCGCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGGCGCGTCGGCAATTTCCGGCAGAACCCAGACGTTATCTTCGTCGATTAATGCCCGGTAACCGAAGAAGTCATCGAATGCCTGAATTTTCAGTTGATTGGTGCTGTTGCGGATCACCGCATACGGACCGGTGCCGATGGGATGGCTGGCAAAGTTACTGAGGGTTCCCCAt                                                               <  2:469820/251‑1 (MQ=255)                                                                                                                                                                                        cAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGGCTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCCCGGTAACCGAAGAAGTCATCGAATGCCTGAATTTTCAGTTGATTGGTGCTGTTGCGGATCACCGCATACGGACCGGTGCCGATGGGATGGCTGGCAAAGTTACTGAGGGTTTCCCATTCGCGCgg                                                       <  1:174369/251‑1 (MQ=255)                                                                                                                                                                                        cAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCGTTTAGCATCAGACCTCCAGCTGGCTCGTCGGCAATTGTCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCCCGGTAACCGAAGAAGTCATCGAATGCCTGAAGTTTCAGTTGATTGGTGCTGTTGCGGATCACCGCATACGGACCGGTGCCGATGGGATGGCTGGCAAAGTTACTGAGGGTTTCCCATTCGCGCgg                                                       <  2:82413/251‑1 (MQ=255)                                                                                                                                                                                           ccTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGGCTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCCCGGTAACCGAAGAAGTCATCGAATGCCTGAATTTTCAGTTGATTGGTGCTGTTGCGGATCACCGCATACGGACCGGTGCCGATGGGATGGCTGGCAAAGTTACTGAGGGTTTCCCATTCGCGCGGCAg                                                    <  1:320847/251‑1 (MQ=255)                                                                                                                                                                                                    aGGCGGCTTTCAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGGCTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGCTTAATGCCCGGTAACCGAAGAAGTCATCGAATGCCTGAATTTTCAGTTGATTGGTGCTGTTGCGGATCACCGCATACGGAACGGTGCCGATGGGATGGCTGGCAAAGTTACTGAGGGTTTCCCATTCGCGCGGCAGGATCATCGc                                           >  1:345590/1‑251 (MQ=255)                                                                                                                                                                                                              cAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGGCTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCCCGGTAACCGAAGAAGTCATCGAATGCCTGAATTTTCAGTTGATTGGTGCTGTTGCGGATCa                                                                                                                       <  1:417592/165‑1 (MQ=255)                                                                                                                                                                                                              cAATCTCTTTTTCCTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGGCTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCCCGGTAACCGAAGAAGTCATCGAATGCCTGAATTTTCAGTTGATTGGTGCTGTTGCGGATCa                                                                                                                       >  2:417592/1‑165 (MQ=255)                                                                                                                                                                                                                          ccTCACCCTGTGGACCTTTTAGCATCAGACCTCCAGCTGGCTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCCCGGTAACCGAAGAAGTCATCGAATGCCTGAATTTTCAGTTGATTGGTGCTGTTGCGGATCACCGCATACGGCCCGGTGCCGATGGGATGGCTGGCAAAGTTACTGCGGGTTTCCCATTCGCGCGGCAGGATCATCGCCGGAACTTGCCCCAGCAATAAc                     >  2:285955/1‑251 (MQ=255)                                                                                                                                                                                                                                ccTGTGGACCTTTTAGCATCAGACCTCCAGCTGGCTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCCCGGTAACCGAAGAAGTCATCGAATGCCTGAATTTTCAGTTGATTGGTGCTGTTGCGGATCACCGCATACGGACCGGTGCCGATGGGATGGCTGGCAAAGTTACTGAGGGTTTCCCATTCGCGCGGCAGGATCATCGCCGGAACTTGCCCCAGCAATAACGGTAAc               >  1:394285/1‑251 (MQ=255)                                                                                                                                                                                                                                    tgGACCTTTTAGCATCAGACCTCCCGCTGGCTCGTCGGCAATTTCCGGCTGAAACCAGACGTTAACTTCGTCGATTAATGCCCGGTAACCGAAGAAGTCATCGAATGCATGAATTTTCAGTTGCTTGGTGCTGATGCGGATCACCGCATACGGACCGGTGACGATGGGATGGCTGGCAAAGTTCCTGAGGGTTTCCCATTAGCGCGGCAGGATCATCGCCGGCACTTGCCCAAGCAATACAGGTAACCAGc           >  2:279018/1‑251 (MQ=255)                                                                                                                                                                                                                                            ttAGCATCAGACCTCCAGCTGGCTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCCCGGTAACCGAAGAAGTCATCGAATGCCTGAATTTTCAGTTGATTGGTGCTGTTGCGGATCACCGCATACGGACCGGTGCCGATGGGATGGCTGGCAAAGTTACTGAGGGTTTCCCATTCGCGCGGCAGGATCATCGCCGGAACTTGCCCCAGCAATAACGGTAACCAGCGGTCCgg    <  1:42548/250‑1 (MQ=255)                                                                                                                                                                                                                                              aGCATCAGCCCTCCAGCTGGCTCGTCGGCAAATTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCCCGGTAACCGAAGAAGTCATCGAATGCCTGAATTTTCAGTTGATTGGTGCTGTTGCGGATCACCGCATACGGACCGGTGCCGATGGGATGGCTGGCAAAGTTACTGAGGGTTTCCCATTCGCGCGGCAGGATCATCGCCGGAACTTGCCCCAGCAATAACGGTAACCAGCGGTCCGGtt  <  2:8375/250‑1 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                  TCGCTCTTTATGGTGCGGGCATGGTGCCAACGGGGGAGCAGTCCATAAGCCGGGAACCACAGTTGCTGGTACTGTTCCTCAGCGAAATAGACCAGATTAGTTGGAGAAAGCACATAGCTTACCCAGTCCCTGACTTGCTGATTCGCCCCGCGATGGGTGCGGCTGTCGAACAGTAAATAGTAGCAACCTTCCTCCAGGCGGCTTTCAATCTCTTTTTCCTCGCCCTGTGGACCTTTTAGCATCAGCCCTCCGGCTGGCTCGTCGGCAATTTCCGGCAGAACCCAGACGTTAACTTCGTCGATTAATGCCCGGTAACCGAAGAAGTCATCGAATGCCTGAATTTTCAGTTGATTGGTGCTGTTGCGAATCACCGCATACGGACCGGTGCCGATGGGATGGCTGGCAAAGTTACTGAGGGTTTCCCATTCGCGCGGCAGGATCATCGCCGGAACTTGCCCCAGCAGTAACGGTAACCAGCGGTCCGGTT  >  NC_000913/76113‑76599

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑