Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,780,060	G→A	H889H (CAC→CAT)	ypjA ←	adhesin‑like autotransporter YpjA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,780,060	0	G	A	97.7%	105.9 / NA	45	H889H (CAC→CAT)	ypjA	adhesin‑like autotransporter YpjA
Reads supporting (aligned to +/- strand):  ref base G (0/1);  major base A (30/13);  minor base C (1/0);  total (31/14)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGAGATCTTCTGCCCGGGCCAGAGCCTGAAACTCATCTTTTGACCGCCCAAGGGTATATTGCCCGCCAGAGTTAACCTTTGTGGCGGAGTCCTGACCCAGGTTTTGCATTGCCCCACCCTTGCCAACCGTGGAGTCGCGAGCTTCGGTACCTGCTAATACCAA‑TAAATTACCGCCATTTTCCAGCAACATATTGGTCGCTAAATTGCCGGAAATGGAAAAAGTACCGTACTGGTGAGTACCGCTGATTTCAATACCGTTAGCCGTGCTCGTCTGGAGAGCGGCACCGCTGTTCTGGACGATATCTGTCGCTTTGCCATTATCGTTGACTGTCAGCGTACCGCCTTCATTGATCTTTGTTTTTATTGCCTCTCCGTTAGCTGAAACTGTTTGTATTCCGCCGTCGTTAATTGTTGTCTCATTCGCCACACCCTCGACAATTTGTTCACCGCCGGTGAGCGTCGTGCCTGTC  >  NC_000913/2779828‑2780297                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                              ggAGATCTTCTGCCCGGGCCAGAGCCTGAAACTCATCTTTTGACCGCCCAAGGGTATATTGCCCGCCAGAGTTAACCTTTGTGGCGGAGTCCTGACCCAGGTTTTGCATTGCCCCACCCTTGCCAACCGTGGAGTCGCGAGCTTCGGGACCTGCTAATACCAA‑TAAATTACCGCCATTTTCCAGCAACATATTGGTCGCTAAATTGCCGGAAATGGAAAAAGTACCGTACTGATGAGTACCGCTGATTTc                                                                                                                                                                                                                              >  1:162017/1‑250 (MQ=255)            gCCCGGGCAAGATCCTGAAACTCATCTTTTGACCGCCCAAGGGTATATTGCCCGCCAGAGTTAACCTGTGTGGCGGAGTCCTGCCCCAGTTTTTGCATTGCCCCACCCTTGCCAACCGTGGAGTCGCGAGCTTCGGTACCTGCTAATACCAA‑TAAATTACCGCCATTTTCCAGCAACATATTGGTCGCTAAATTGCCGGAAATGGAAAAAGTACCGTTCTGATGAGTACCGCTTATTTCAATACCGTTa                                                                                                                                                                                                                    <  1:356420/249‑1 (MQ=255)               cGGGCCAGAGCCTGAAACTCATCTTTTGACCGCCCAAGGGTATATTGCCCGCCAGAGTTAACCTTTGTGGCGGAGTCCTGACCCAGGTTTTGCATTGCCCCACCCTTGCCAACCGTGGAGTCGCGAGCTTCGGTACCTGCTAATACCAA‑TAAATTACCGCCATTTTCCAGCAACATATTGGTCGCTAAATTGCCGGAAATGGAAAAAGTACCGTACTGATGAGTACCGCTGATTTCAATACCGTTAGCCGt                                                                                                                                                                                                               >  1:18831/1‑251 (MQ=255)                             aaaCTCATCTTTTGACCGCCCAAGGGTATATTGCCCGCCAGAGTTAACCTTTGTGGCGGAGTCCTGACCCAGGTTTTGCATTGCCCCACCCTTGCCAACCGTGGAGTCGCGAGCTTCGGTACCTGCTAATACCAA‑TAAATTACCGCCATTTTCCAGCAACATATTGGTCGCTAAATTGCCGGAAATGGAAAAAGTACCGTACTGATGAGTACCGCTGATTTCAATAACGTTAGCCGTGCTCggcttgag                                                                                                                                                                                                   >  2:334940/1‑242 (MQ=255)                                     cTTTTGACCGCCCAAGGGTATATTGCCCGCCAGAGTTAACCTTTGTGGCGGCGTCCTGACCCAGGTTTTGCATTGCCCCACCCTTGCCCACCGTGGAGTCGCGAGCTTCGGTACCTGCTAATACCAA‑TAAATTACCGCCATTTTCCACCAACATATTGGTCGCTAAATTGCCGGAAATGGAAAAAGTACCGTACTGATGAGTACCGCTGATTTAAATACCGTTAGCCGTGCTCGTCTGGAGAGCGGCGCCg                                                                                                                                                                                         >  2:45701/1‑251 (MQ=255)                                         agACCGCCCAAGGGTATATTGCCCGCCAGAGTTAACCTTTTTGGCGGAGTCCTGACCCAGGTTTTGCCTTGCCCCACCCTTTCCCACCGTGGAGTCGCGGGCTTCGGTACCTGCTAATACCCA‑TAAATTCCCGCCATTTTCCAACAACATTATGGTCTCTACATTGCCGGCACTGGAACAAGTACAGTACTGCTGAGTACCTCTGGTTTCAATCCCGTTAGCAGTGCTCGTCTGGAGAGCGGCCCCGCTGt                                                                                                                                                                                     >  2:3378/2‑251 (MQ=255)                                                       tataTTGCCCGCCCGAGTTCACCTTTGTGGCGGCGTCCTTACCCATGTTTTGCCTTGCCCCACCCTTGCCAACCGTGGAGTCGCGAGCTTCGGTACCTGCTAATACCAA‑TAAATTACCGCCCTTTTCCAGCAACATATTGGTCGCTAAATTGCCGGAAATTGAAAAAGTACCGTACTGATTGGTACCGCTTATTTCAATACCGTTATCCGTGCTCGTCTGGAGCGCGGCCCCGCTGTTCTTGACGatatat                                                                                                                                                                       >  2:315442/1‑249 (MQ=255)                                                              cccGCCAGAGTTAACCTTTGTGGCGGAGTCCTGACCCAGGTTTTGCATTGCCCCACCCTTGCCAACCGTGGAGTCGCGAGCTTCGGTACCTGCTAATACCAA‑TAAATTACCGCCATTTTCCAGCAACATATTGGTCGCTAAATTGCCGGAAATGGAAAAAGTACCGTACTGATGAGTACCGCTGATTTCAATACCGTTAGCCGTGCTCGTCTGGAGAGCGGCACCGCTGTTCTGGACGATATCTGTCGCtt                                                                                                                                                                >  1:380831/1‑251 (MQ=255)                                                                      agTTAACCTTTGTGGCGGAGTCCTGACCCAGGTTTTGCATTGCCCCACCCTTGCCAACCGTGGAGTCGCGAGCTTCGGTACCTGCTAATACCAA‑TAAATTACCGCCATTTTCCAGCAACATATTGGTCGCTAAATTGCCGGAAATGGAAAAAGTACCGTACTGATGAGTACCGCTGATTTCAATACCGTTAGCCGTGCTCGTCTGGAGAGCGGCACCGCTGTTCTGGACGATATCTGTCGCTTTGCCATTa                                                                                                                                                        <  1:334940/251‑1 (MQ=255)                                                                        tttcccTTTGTGGTTGAGTCCTGTTCCTGTTTTTCCATTCCCCCACCCTTGCCACCCTGGGAGCCGGTAGCTCCGTTACCTGATA‑TAACAACTAAATAACCGCCATTTGCCAGCAACATATTGGTCGCTATATTGCCGGAAAGGGAAAACGTACCTTACTGATGAGTACCGCTGATTTCAATACCGTTAGCCGTGCTCGTCTGGAGAGCGGCGCCGCTGTTCTGGACGATATCTGTCGCTTTGCCATTAt                                                                                                                                                       <  2:364671/246‑1 (MQ=255)                                                                              tttGTGGCGGAGTCCTGACCCAGGTTTTGCATTGCCCCACCCTTGCCAACCGTGGAGTCGCGAGCTTCGGTACCTGCTAATACCAA‑TAAATTACCGCCATTTTCCAGCCACATATTGGTCGCTAAATTGCCGGAAATGGAAAAAGTACCGTACTGATGAGTACCGCTGTTTTCAATACCGTTAGCCGTGCTCGTCTGGAGAGCGGCCCCGCTGTTCTGGACGATATCTGTCGCTTTGCCCTTATCGTTGAc                                                                                                                                                >  2:109397/1‑251 (MQ=255)                                                                                         gTCCTGACCCAGGTTTTGCATTGCCCCACCCTTGCCAACCGTGGAGTCGCGAGCTTCGGTACCTGCTAATACCAA‑TAAATTACCGCCATTTTCCAGCAACATATTGGTCGCTAAATTGCCGGAAATGGAAAAAGTACCGTACTGATGAGTACCGCTGATTTCAATACCGTTAGCCGTGCTCGTCTGGAGAGCGGCACCGCTGTTCTGGACGATATCTGTCGCTTTGCCATTATCGTTGACTGTCAGCGTAc                                                                                                                                     >  2:73153/1‑251 (MQ=255)                                                                                           ccTGCCCCAGGTTTTGCATTGCCCCCCCCTTGCCAACCGTGGAGTCGCGAGCTTCGGTCCCTGCTAATACCAA‑TAAATTACCGCCATTTTCCAGCAACATATTGGTCGCTAAATTGCCGGAAATGGAAAAAGTACCGTACTGATGAGTACCGCTGATTTCAATACCGTTAGCCGTGCTCGTCTGGAGAGCGGCACCGCTGTTCTGGACGATATCTGTCGCTTTGCCATTATCGTTGACTGTCAGCGTACCg                                                                                                                                   <  1:73153/251‑1 (MQ=255)                                                                                                             ttGCCCCCCCCTTGCCACCCGTGGGGCCGCGATCGCCGCTACCTCCTAATCCCAA‑AAAATTACCGTCATTTTCCAGCAACATATTGGTCGCTAAATTGCCGGAAATGGAAAAAGTACCGTACTGATGAGTCCCGCTGATTTCAATACCGTTAGCCGTGCTCGTCTGGAGAGCGGCACCGCTGTTCTGGACGATATCTGTCGCTTTGCCATTATCGTTGACTGGCAGCGTACCGCCTTCATTGATCTTTGt                                                                                                                  <  2:18831/250‑1 (MQ=255)                                                                                                              tGCCCCACCCTTGCCAACCGTGGAGTCGCGAGCTTCGGTACCTGCTAATACCAA‑TAAATTACCGCCATTTTCCAGCCACATATTGGTCGCTAAATTGCCGGAAATGGAAAAAGTACCGTACTGATGAGTACCGCTGATTTCAATACCGTAAGCCGTGCTGGTCTGGAGAGCGGCACCGCTGTTCTGGACGATATCTGTCGCTTTGCCATTATCGTTGACTGTCAGCGTCCCGCCTTCATTATTCTTTGttt                                                                                                                >  2:410067/1‑251 (MQ=255)                                                                                                                            cAACCGTGGAGTCGCGAGCTTCGGTACCTGCTAATACCAA‑TAAATTACCGCCATTTTCCAGCAACATATTGGTCGCTAAATTGCCGGAAATGGAAAAAGTACCGTACTGATGAGTACCGCTGATTTCAATACCGTTAGCCGTGCTCGTCTGGAGAGCGGCCCCGCTGTTCTGGACGATATCTGTCGCTTTGCAATTATCGTTGACTGTACGCGTACCGCCTTCATTGATCTTTGTTTTTATTGCCTCTCCg                                                                                                  >  2:342337/1‑251 (MQ=255)                                                                                                                            cAACCGTGGAGTCGCGAGCTTCGGTACCTGCTAATACCAA‑TAAATTACCGCCATTTTCCAGCAACATATTGGTCGCTAAATTGCCGGAAATGGAAAAAGTACCGTACTGATGAGTACCGCTGATTTCAATACCGTTAGCCGTGCTCGTCTGGAGAGCGGCACCGCTGTTCTGGCCGATATCTGTCGCTTTGCCCTTATCGTTGACTGTCAGCGTACCGCCTTCATTGCTCTTTGTTTTTCTTGCCTCTccc                                                                                                  >  2:112123/1‑250 (MQ=255)                                                                                                                                         gcgAGCTTCGGTACCTGCTAATACCAA‑TAAATTACCGCCATTTTCCAGCAACATATTGGTCGCTAAATTGCCGGAAATGGAAAAAGTACCGTACTGATGAGTACCGCTGATTTCAATACCGTTAGCCGTGCTCGTCTGGAGAGCGGCACCGCTGTTCTGGACGATATCTGTCGCTTTGCCATTATCGTTGACTGTCAGCGTACCGCCTTCATTGATCTTTGTTATTATTGCCTCTCCGTTAGCTGAACCTg                                                                                     >  2:316954/1‑251 (MQ=255)                                                                                                                                                tCGGTACCTGCTAATACCAA‑TAAATTACCGCCATTTTCCAGCAACATATTGGTCGCTAAATTGCCGGAAATGGAAAAAGTACCGTACTGATGAGTACCGCTGATTTCAATACCGTTAGCCGTGCTCGTCTGGAGAGCGGCACCg                                                                                                                                                                                         >  1:358545/1‑144 (MQ=255)                                                                                                                                                 cGGTAACTGCTAATACCAA‑TAAATTACCGCCATTTTCCAGCAACATATTGGTCGCTAAATTGCCGGAAATGGAAAAAGTACCGTACTGATGAGTACCGCTGATTTCAATACCGTTAGCCGTGCTAGGCTGGAGAGCGGCACCGCTGTTCTGAACGATATCTGTCGCTTTGCCATTATCGTTGACTGTCAGCGTACCGCCTTCATTGATCTTTGTTTTTATTGCCTCTCCGTTAGCTGAAACTGTTTGTAtt                                                                             >  2:65711/1‑251 (MQ=255)                                                                                                                                                  ggTACCTGCTAATACCAA‑TAAATTACCGCCATTTTCCAGCAACATATTGGTCGCTAAATTGCCGGAAATGGAAAAAGTACCGTACTGATGAGTACCGCTGATTTCAATACCGTTAGCCGTGCTCGTCTGGAGAGCGGCACCGCTGTTCTGGACGATATCTGTCGCTTTGCCATTATCGTTGACTGTCAGCGTACCGCCTTCATTGATCTTTGTTTTTATTGCCTCTCCGTTAGCTGAAACTGTTTGTATTc                                                                            >  1:136102/1‑251 (MQ=255)                                                                                                                                                        tGCTAATACCAA‑TAAATTACCGCCATTTTCCAGCAACATATTGGTCGCTAAATTGCCGGAAATGGAAAAAGTACAGTACTGATGAGTACCGCTGATTTCAATACCGTTAGCCGTGCTCGTCTGGAGAGCGGCACCGCTGTTCTGGACGATATCTGTCGCTTTGCCATTATCGTTAACTGTCAGCGTACCGCCTTCATTGATCTTTGTTTTTCTTGCCTCTCCGTTAGCTGAAACTGTTTGTATTCCGCcgt                                                                      >  2:458414/1‑251 (MQ=255)                                                                                                                                                         gCTAATCCCAA‑TAAATTACCGCCATTTTCCAGCAACATATTGGTCGCTAAATTGCCGGAAATGGAAAAAGTACCGTACTGATGAGTACCGCTGATTTCAATACCGTTAGCCGTGCTCGTCTGGAGAGCGGCACCGCTGTTCTGGACGATATCTGTCGCTTTGCCATTATCGTTGACTGTCAGCGTACCGCCTTCATTGATCTTTGTTTTTATTGCCTCTCCGTTAGCTGAAACTGTTTGTATTCCGCcgt                                                                      <  1:414102/250‑1 (MQ=255)                                                                                                                                                                  aacaaatttACCGCCATTTTCCAGAAACATATTGGTCGCTAAATTGCCGGAAATGGAAAAAGTACCGTACTGATGAGTACCGCTGATTTCAATACCGTTAGCCGTGCTCGTCTGGAGAGCGGCACCGCTGTTCTGGACGATATCTGTCGCTTTGCCATTATCGTTGACTGTCAGCGTACCGCCTTCATTGATCTTTGTTTTTATTGCCTCTCCGTTAGCTGAAACTGTTTGTATTCCTCCGTCGTTAAttg                                                             <  2:162017/244‑1 (MQ=255)                                                                                                                                                                   a‑TAAATTACCGCAATTTTCCAGCAACATATTGGTCGCTAAATTGCCGGAAATGGAAAAAGTACCGTACTGATGAGTACCGCTGATTTCAATACCGTTAGCCGTGCTCGTCTGGAGAGCGGCACCGCTGTTCTGGACGATATCTGTCGCTTTGCCATTATCGTTGACTGTCAGCGTACCGCCTTCATTGATCTTTGTTTTTATTGCCTCTCCGTTAGCTGAAACTGTTTGTATTCCGCCGTCGTTAAttgtt                                                           >  1:147826/1‑251 (MQ=255)                                                                                                                                                                     aaaaTTACCGCCATTTTCCAGCAACATATTGGTCGCTAAATTGCCGGAAATGGAAAAAGTACCGTACTGGTGAGTACCGCTGATTTCAATAACGTTAGCCGTGCTCGTCTGGAGAGCGGCACCGCTGTTCTGGACGATATCTGTCGCTTTGCCATTATCGTTGACTGTCAGCGTACCGCCTTCATTGATCTTTGTTTTTATTGCCTCTCCGTTAGCTGAAACTGTTTGTATTCCGCCGTCGTTAAttgttg                                                          <  1:210073/250‑1 (MQ=255)                                                                                                                                                                     aaaaTTACCGCCATTTTCCAGCAACATATTGGTCGCTAAATTGCCGGAAATGGAAAAAGTACCGTACTGATGAGTACCGCTGATTTCAATACCGTTAGCCGTGCTCGTCTGGAGAGCGGCACCGCTGTTCTGGACGATATCTGTCGCTTTGCCATTATCGTTGACTGTCAGCGTACCGCCTTCATTGATCTTTGTTTTTATTGCCTCTCCGTTAGCTGAAACTGTTTGTATTCCGCCGTCGTTAAttgttg                                                          <  1:5438/250‑1 (MQ=255)                                                                                                                                                                      aatgagccGCCATTTTCCAGCAACATATTTGTCGCTAAATTGCCGGAAATGGAAAAAGTTCCGTACTGATGAGTACCGCTTATTTCAATTCCGTTAGCCTTTCTCGTCTGGAGTGCGGCACCGCTGTACTGGACGATATCTGTCGCTTTGCCTTTATCGTTGACTGTCAGCGTACCGCCTTCATTGATCTTTGTTTTTATTGCCTTTCCGTTAGCTTAAACTGTTTGTATTCCGCCGTCGTTAATtgttgt                                                         <  1:424990/245‑1 (MQ=255)                                                                                                                                                                         ttACCGCCATTTTCCAGCAACATATTGGTCGCTAAATTGCCGGAAATGGAAAAAGTACCGTACTGATGAGTACCGCTGATTTCAATACCGTTAGCCGTGCTCGTCTGGAGAGCGGCACCGCTGTTCTGGACGATATCTGTCGCTTTGCCATTATCGTTGACTGTCAGCGTACCGCATTCATTGATCTTTGTTTTTATTGCCTCTCCGTTAGCTGAAACTGTTTGTATTCCGCCGTCGTTACTTGTTGtctc                                                      >  1:256402/1‑251 (MQ=255)                                                                                                                                                                              gCCATTTTCCAGCAACATATTGGTCGCTAAATTGCCGGAAATGGAAAAAGTACCGTACTGATGAGTACCGCTGATTTCAATACCGTTAGCCGTGCTCGTCTGGAGAGCGGCACCGCTGTTCTGGATGATATCTGTCGCTTTGCCATTATCGTTGACTGTCAGCGTACCGCCTTCATTGATCTTTGTTTTTATTGCCTCTCCGTTAGCTGAAACTGTTTGTATTCCGCCGTCGTTAATTGTTGTCTCATTCg                                                 >  1:208409/1‑251 (MQ=255)                                                                                                                                                                                 aTTTTCCAGCAACATATTGGTCGCTACATTGCCGGAAATGGAAAAAGTACCGTACTGATGAGTACCGCTGCTTTCAATACCGTTAGCCGTGCTCGTCTGGAGAGCGGCACCGCTGTTCTGGACGATATCTGTCGCTTTGCCATTATCGTTGACTGTCAGCGTACAGCCTTCATTGATCTTTGTTTTTATTGCATCTCAGTTAGCTGAAACTGTTTGTTTTTCGACGTCGTTAATTGTTGTCTCATTCGccc                                              >  2:314001/1‑250 (MQ=255)                                                                                                                                                                                    ttCCAGCCACATATTGGTCGCTAAATTGCCGGAAATGGAAAAAGTACCGTACTGATGAGTACCGCTGATTTCAATACCGTTAGCCGTGCTCGTCTGGAGAGCGGCACCGATGTTCTGGCCGCTATCTGTCGCTTTGCCAGTATCGTTGACTGTCAGCGAACCGCCTTCATTGATCTTTGTTTTTTTTGCCTCTCCGTTAGCTGACACTGTTTGTATTTAGCCGTCGTTATTTGTTGTTTCATTCGCCacac                                           >  2:121393/1‑251 (MQ=255)                                                                                                                                                                                         gCAACATATTGGTCGCTAAATTGCCGGAAATGGAAAAAGTACCGTACTGATGAGTACCGCTGATTTCAATACCGTTAGCCGTGCTCGTCTGGAGAGCGGCACCGCTGTTCTGGACGATATCTGTCGCTTTGCCATTATCGTTGACTGTCAGCGTACCGCCTTCATTGATCTTTGTTTTTATTGCCTCTCCGTTAGCTGAAACTGTTTGTATTCCGCCGTCGTTAATTGTTGtctc                                                      <  2:1679/235‑1 (MQ=255)                                                                                                                                                                                         gCAACATATTGGTCGCTAAATTGCCGGAAATGGAAAAAGTACCGTACTGATGAGTACCGCTGATTTCAATACCGTTAGCCGTGCTCGTCTGGAGAGCGGCACCGCTGTTCTGGACGATATCTGTCGCTTTGCCATTATCGTTGACTGTCAGCGTACCGCCTTCATTGATCTTTGTTTTTATTGCCTCTCCGTTAGCTGAAACTGTTTGTATTCCGCCGTCGTTAATTGTTGtctc                                                      >  1:1679/1‑235 (MQ=255)                                                                                                                                                                                          caacctagtgggcGCTAAATTGCCGGAAATGGAAAAAGTTCCGTACTGAGGAGTACCGCTGATTTCAAGCCCGTTAGCCGTCGTCGTCTGTAGAGCGTCGCCGCTGTTCTGGACGATATCTGTCGCTTTGCCATTATCGTTGACTTTCAGCGTACCGCCTTCATTGATCTTTGTTTTTATTGACTCGCCGTTAGCTGAAACTGTTTGTACTCCGCCGTCGTTAATTGTTGTCACATTCGcccctcccgc                                       <  2:253241/237‑9 (MQ=255)                                                                                                                                                                                           aaCATATTGGTCGCTAAATTGCCGGAAATGGAAAAAGTACCGTACTGATGAGTACCGCTGCTTTCAATACCGTTAGCCGTGCTCGTCTGGAGAGCGGCACCGCTGTTCTGGACGATATCTGTCGCTTTGCCATTATCGTTGACTGTCAGCGTACCGCCTTCATTGATCTTTGTTTTTATTGCCTCTCCGTTAGCTGAAACTGTTTGTATTCCGCCGTCGTTAATTGTTGTCTCATTCGCCACACCCTCGAc                                    >  2:106677/1‑251 (MQ=255)                                                                                                                                                                                                       gCTACATTGCCGGAAATGGAAAAAGTACCGTACTGATGAGTACCGCTGATTTCAATACCGTTAGCCGTGCTCGTCTGGAGAGCGGCACCGCTGTTCTGGCCGATATCTGTCGCTTTGCCATTATCGTTGACTGTCAGCGTACCGCCTTCATTGATCTTTGTTTTTATTGCCTCTCCGTTAGCTGAAACTGTTTGTATTCCGCCGTCGTTAATTGTTGTCTCCTTAGCCACACCCTCTACATTTTGTTCAcc                        >  2:9424/1‑251 (MQ=255)                                                                                                                                                                                                             ttGCCGGAAATGGAAAAAGTACCGTACTGATGAGTACCGCTGATTTCAATACCGTTAGCCATGCTCGTCTGGAGAGCGGCACCGCTGTTCTGGACGATATCTGTCGCTTTGCCATTATCGTTGACTGTCAGCGTACCGCCTTCATTGATCTTTGTTTTTATTGCCTCTCCGTTAGCTGAAACTGTTTGTATTCCGCCGTCGTTAATTGTTGTCTCATTCGCCACACCCTCGACAATTTGTTCACCGCCGGt                  >  1:175250/1‑251 (MQ=255)                                                                                                                                                                                                              tGCCGGAAATGGAAAAAGTACCGTACTGATGAGTACCGCTGATTTCAATACCGTTAGCCGTGCTCGTCTGGAGAGCGGCACCGCTGTTCTGGACGATATCTGTCGCTTTGCCATTATCGTTGACTGTCAGCGTACCGCCTTCATTGATCTTTGTTTTTATTGCCTCTCCGTTAGCTGAAACTGTTTGTATTCCGCCGTCGTTAATTGTTGTCTCATTCGCCACACCCTCGACAATTTGTTCACCGCCGGTg                 >  2:127351/1‑251 (MQ=255)                                                                                                                                                                                                              tGCCGGAAATGGAAAAAGTACCGTACTGATGAGTACCGCTGATTTCAATACCGTTAGCCGTGCTCGTCTGGAGAGCGGCACCGCTGTTCTGGACGATATCTGTCGCTTTGCCATTATCGTTGACTGTCAGCGAACCGCCTTCATTGATCTTTGTTTTTATTGCCTCTCCGTTAGCTGAAACTGTTTGTATTCCGCCGTCGTTAATTGTTGTCTCATTCGCCACACCCTCGACAATTTGTTCAccgccgttg                 >  2:316151/1‑248 (MQ=255)                                                                                                                                                                                                                ccGGAAATGGAAAAAGTACCGTACTGATGAGTACCGCTGATTTCAATACCGTTAGCCGTGCTCGTCTGGAGAGCGGCACCGCTGTTCTGGACGATATCTGTCGCTTTGCCATTATCGTTGACTGTCAGCGTACCGCCTTCATTGATCTTTGTTTTTATTGCCTCTCCGTTAGCTGAAACTGTTTGTATTCCGCCGTCGTTAATTGTTGTCTCATTCGCCACACCCTCGACAATTTGTTCACCGCCGGTg                 >  1:345993/1‑249 (MQ=255)                                                                                                                                                                                                                      aTGGAAAAAGTCCCGTTCTGATGAGTACCGCGGATTTCAATCCCGTTCGCCGTGCTCGTTGGGAGAGCGGCACCGCTGTTCTGGACGATATCTGTCGCTTTGCCATTATCGTTGATTGTCAGCGTACCGCCTTCATTGATCTTTGTTTTTATTGCCTCTCCGTTAGCTAAAACTGTTTGTATTCCGCCGTCGTTAATTGTTGTCTCATTCGCCACACCCTCGACAATTTGTTCACCGCCGGTGAGcgtcgt         <  1:143893/251‑1 (MQ=255)                                                                                                                                                                                                                      aTGGAAAAAGTACCGTACTGATGAGTACCGCTGATTTCAATACCGTTAGCCGTGCTCGTCTGGAGAGCGGCACCGCTGTTCTGGACGATATCTGTCGCTTTGCCATTATCGTTGACTGTCAGCGTACCGCTTTCATTGATCTTTGTTTTTATTGCCTCTCCGTTAGCTGAAACTGTTTGTATTCCGCCGTCGTTAATTGTTGTCTCATTCGCCACACCCTCGACAATTTGTTCACCGCCGGTGAGcgtcgt         <  1:378311/251‑1 (MQ=255)                                                                                                                                                                                                                            aaaGTACCGTACTGATGAGTACCGCTGATTTCAATACCGTTAGCCGTGCTCGTCTGGAGAGCGGCACCGCTGTTCTGGACGATATCTGTCGCTTTGCCATTATCGTTGACTGTCAGCGTACCGCCTTCATTGATCTTTGTTTTTATTGCCTCTCCGTTAGCTGAAACTGTTTGTATTCCGCCGTCGTTAATTGTTGTCTCATTCGCCACCCCCTCGACAATTTGTTCACCGCCGGTGAGCGTCGTGCCTGt   >  2:24229/1‑251 (MQ=255)                                                                                                                                                                                                                             aaGTACCGTACTGATGAGTACCGCTGATTTCAATACCGTTAGCCGTGCTCGTCTGGAGAGCGGCACCGCTGTTCTGGACGATATCTGTCGCTTTGCCATTATCGTTGACTGTCAGCGTACCGCCTTCCTTGATCTTTGTTTTTATTGCCTCTCCGTTAGCTGCAACTGTTTGTATTCCGCCGTCGTTAATTGTTGTCTCATTCGCCACACCCTCGACACTTTGTTCACCGCCGGTGAGCGTCGTGCCTGTc  >  2:199058/1‑251 (MQ=255)                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                              GGAGATCTTCTGCCCGGGCCAGAGCCTGAAACTCATCTTTTGACCGCCCAAGGGTATATTGCCCGCCAGAGTTAACCTTTGTGGCGGAGTCCTGACCCAGGTTTTGCATTGCCCCACCCTTGCCAACCGTGGAGTCGCGAGCTTCGGTACCTGCTAATACCAA‑TAAATTACCGCCATTTTCCAGCAACATATTGGTCGCTAAATTGCCGGAAATGGAAAAAGTACCGTACTGGTGAGTACCGCTGATTTCAATACCGTTAGCCGTGCTCGTCTGGAGAGCGGCACCGCTGTTCTGGACGATATCTGTCGCTTTGCCATTATCGTTGACTGTCAGCGTACCGCCTTCATTGATCTTTGTTTTTATTGCCTCTCCGTTAGCTGAAACTGTTTGTATTCCGCCGTCGTTAATTGTTGTCTCATTCGCCACACCCTCGACAATTTGTTCACCGCCGGTGAGCGTCGTGCCTGTC  >  NC_000913/2779828‑2780297

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑