Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,841,238 G→A M83I (ATG→ATA)  ascB → 6‑phospho‑beta‑glucosidase AscB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,841,2380GA93.3% 77.5 / ‑1.3 30M83I (ATG→ATA) ascB6‑phospho‑beta‑glucosidase AscB
Reads supporting (aligned to +/- strand):  ref base G (1/1);  new base A (14/14);  total (15/15)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.80e-01

GTCAGTATTTCCAGAAAGTTTTTTATGGGGCGGCGCGCTTGCCGCCAACCAGTCTGAAGGTGCGTTCCGTGAAGGTGACAAAGGTCTGACCACTGTCGATATGATCCCACACGGCGAGCATCGAATGGCGGTGAAACTGGGGCTGGAAAAACGTTTTCAGTTGCGAGATGACGAGTTTTATCCCAGCCATGAGGCGACGGATTTTTATCATCGTTATAAAGAAGATATCGCCCTGATGGCAGAGATGGGATTCAAGGTTTTCCGTACCTCAATTGCCTGGAGCCGTCTCTTTCCGCAGGGCGATGAAATCACGCCCAATCAGCAGGGCATTGCTTTTTATCGTTCTGTCTTTGAAGAGTGTAAAAAGTACGGTATCGAACCGCTGGTCACGTTGTGCCACTTCGATGTGCCGATGCATCTGGTCACCGAATATGGCTCCTGGCGTAACCGCAAGCTGGTGGAGTTTTTCAGCCGCTACGC  >  NC_000913/2840992‑2841471
                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                         
gTCAGTATTTCCAGAAAGTTTTTTATGGGGCGGCGCGCTTGCCGCCAACCAGTCTGAAGGGGCGTTCCGTGAAGGGGACAAAGGTCTGACCACTGTCGATATGATCCCACACGGCGAGCATCGAATGGCGGTGGAACTGGGGCTGGAAAAACGTTTTCGGTTTCGGGATGGCGGGTTTTATCACAGCCCTGAGGCGACGGGTTTTTGTCATCGTTTTAAAGAAGATATCGACCTGATGGCAGAGATAGGAt                                                                                                                                                                                                                                       >  1:329677/1‑251 (MQ=255)
   aGTATTTCCAGAAAGTTTTTTATGGGGCGGCGCGCTTGCCGCCAACCAGTCTGAAGGTGCGTTCCGTGAAGGTGACAAAGGTCTGACCACTGTCGATATGATCCCACACGGCGAGCATCGAATGGCGGTGAAACTGGGGCTGGAAAAACGTTTTCAGTTGCGAGATGACGAGTTTTATCCCAGCCATGAGGCGACGGATTTTTATCATCGTTATAAAGAAGATATCGCCCTGATGGCAGAGATAGGATTCa                                                                                                                                                                                                                                    <  1:227180/251‑1 (MQ=255)
      ttttCCAGAAAGTTTTTTATGGGGCGGCGCGCTTGCCGCCAACCAGTCTGAAGGTGCGTTCCGTGAAGGTGACAAAGGTCTGACCACTGTCGATATGATCCCACACGGCGAGCATCGAATGGCGGTGAAACTGGGGCTGGAAAAACGTTTTCAGTTGCGAGATGACGAGTTTTATCCCAGCCATGAGGCGACGGATTTTTATCATCGTTATAAAGAAGATATCGCCCTGATGGCAGAGATAGGATTCAAg                                                                                                                                                                                                                                  <  1:135298/249‑1 (MQ=255)
        ttCCAGAAAGTTTTTTATGGGGCGGCGCGCTTGCCGCAAACCAGTCTGAAGGTGCGTTCCGTGAAGGTGACAAAGGTCTGACCACTGTCGATATGATCCCACACGGCGAGCATCGAATGGCGGTGAAACTGGGGCTGGGAAAACGTTTTCCGTTGCGAGATGACGCGTTTTATCCCCGCCATGAGGCGACGGATTTTTATCATCGTTATTAAGGAGATATCGCCCCGGTGGCAGGGATGGGATTCAAGGtt                                                                                                                                                                                                                               >  1:155521/1‑251 (MQ=255)
                      ttATGAGGCGGCGAGCTTGCCGCCAACCAGACTAAAGGTGCGTTCCGTGAAGGTGACAAAGGTCTGACCACTGTCGATATGATCACACACGGCGAGCATCGAATGGCGGTGAAACTGGGGCTGGAAAAACGTTTTCAGTTGCGAGATGACGAGTTTTATCCCAGCCATGAGGCGACGGATTTTTATCATCGTTATAAAGAAGATATCGCCCTGATGGCAGAGATAGGATTCAAGGGTTTCCGTACATCaa                                                                                                                                                                                                                  >  1:122593/1‑250 (MQ=255)
                          ggggCGGCGCGCTTGCCGCCAACCAGTCTGAAGGTGCGTTCCGTGAAGGTGACAAAGGTCTGACCACTGTCGATATGATCCCACACGGCGAGCATCGAATGGCGGTGAAACTGGGGCTGGAAAAACGTTTTCAGTTGCGAGATGACGAGTTTTATCCCAGACATGAGGCGACGGATTTTTATCATCGTTATAAAGAAGATATCGCCCTGATGGCAGAGATAGGATTCAAGGTTTTCCGTACCTAAATTGcc                                                                                                                                                                                                             >  1:333538/1‑251 (MQ=255)
                                gcgcgcTTGCCGCCAACCATCCTGAAGGTGCGTTCCTTGAAGGTTACAAAGGTTTGACCACTGTCGATATGATCCCTCACGGTGAGCATAGAATGGCGTTTAAACTGGGGCTGGAAAAACGTTTTCAGTTGCGAGATGACGAGTTTGATCCCAGCCATGAGGCGACTGATTTTTATCATCGTTATAAAGAAGATAGCGCCCGGATGGCAGAGTTAGGATTCAATGTTTTCCCTACCTCAATTGCCTGGAGc                                                                                                                                                                                                       <  1:131535/251‑1 (MQ=255)
                                            ccAACCAGTCTGAAGGGGCGTCCCGTGAAGGTGACAAAGGTCTGACCACTGTCGATATGATCCCACACGGCGAGCAGCGAATGGCGGTGAAACTGGGGCTGGAAAAACGTTTTCAGTTGCGAGATGACGAGTTTTATCCCAGCCATGAGGCGACGGATTTTTATCATCGTTATAAAGAAGATATCGCCCTGATGGCAGAGATGGGATTCAAGGTTTTCCGTACCTCAATTGCCTGGAGCCGTCTCTTTCCg                                                                                                                                                                                           <  2:155521/251‑1 (MQ=255)
                                                        aaGGTGCGTTCCGTGAAGGTGACAAAGGTCTGACCACTGTCGATATGATCCCACACGGCGAGCATCGAATGGCGGTGAAACTGGGGCTGGGAAAACGGTTTCAGTTGCGAGGTGACGAGTTTTATCCCAAACCTGAGGCGACGGGATTTTATCATCGTTATAAAGAAAATATCGCCCTGATGGAAGAGATAGGGTTCCAGGTTTTCCGTACCTCAATTGCCTGGAGCCGTCTCTTTCCGCAgggggggtc                                                                                                                                                                                >  2:68748/1‑244 (MQ=255)
                                                                    gTGAAGGTGACAAAGGTCTGACCACTGTCGATATGATCCCACACTGCGAGCATCGAATGGCGGTGAAACTGGGGCTGGAAAAACGTTTTCAGTTGCGAGATGACGAGTTTTATCCCAGCCATGAGGCGGCGGATTTTTAGCATCGTTATAAATAAGATATCGCCCTGATGGCAGAGATAGGATTCAAGGTTTTCCTTACCTCAATTGCCTGGAGCCGTCTCTTTCCGCAGGGCGATGAAATCACGCCCAAt                                                                                                                                                                   <  1:291306/251‑1 (MQ=255)
                                                                       aaGGTGACAAAGGTCTGACCACTGTCGATATGATCCCACACGGCGAGCATCGAATGGCGGTGAAACTGGGGCTGGAAAAACGTTTTCAGTTGCGAGATGACGAGTTTTATCCCAGCCATGAGGCGACGGATTTTTATCATCGTTATAAAGAAGATATCGCCCTGATGGCAGAGATAGGATTCAAGGTTTTCCGTACCTCAATTGCCTGGAGCCGTCTCTTTCCGCAGGGCGATGAAATCACGCCCAATcag                                                                                                                                                                <  1:338148/251‑1 (MQ=255)
                                                                           tGACAAAGGTCTGACCACTGTCGATATGATCCCACACGGCGAGCATCGAATGGCGGTGAAACTGGGGCTGGAAAAACGTTTTCAGTTGCGAGATGACGAGTTTTATCCCAGCCATGAGGCGACGGATTTTTATCATCGTTATAAAGAAGATATCGCCCTGATGGCAGAGATAGGATTCAAGGTTTTCCGTACCTCAATTGCCTGGAGCCGTCTCTTTCCGCAGGGCGATGAAATCACGCCCAATCAGCAgg                                                                                                                                                            >  1:422269/1‑251 (MQ=255)
                                                                                              gTCGATATGATCCCACACGGCGAGCATCGAATGGCGGTGAAACTGGGGCTGGAAAAACGTTTTCAGTTGCGAGATGACGAGTTTTATCCCAGCCATGAGGCGACGGATTTTTATCATCGTTATAAAGAAGATATCGCCCTGATGGCAGAGATAGGATTCAAGGTTTTCCGTACCTCAATTGCCTGGAGCCGTCTCTTTCCGCAGGGCGATGAAATCACGCCCAATCAGCAGGGCATTGCTTTTTATCGtt                                                                                                                                          <  1:61359/250‑1 (MQ=255)
                                                                                                  atatGATCCCACACGGCGAGCATCGAATGGCGGTGAAACTGGGGCTGGACAAACGTTTTCAGTTGCGAGATGTCGAGTTTTATCCCAGCCCTGCGGCGACGGATTTTTATCCTCGTTATAAAGAAGATATCGCCCTGATGGCCGAGATAGGATTCAAGGTTTTCCGTACCTCAATTGCCTGGAGCCGTCTCTTTCCGCATGGCGATGTAATCACGCCCAATCAGCAGGGCCTTGCTTTTTATCGTTCTGTc                                                                                                                                     >  1:167432/1‑251 (MQ=255)
                                                                                                                   gAGCATCGAAGGGCGGTGAAACTGGGGCTGGAAAAACGTATGTGGTGTCGAGATGGCTAGATTTAGCCCAGCCATGAGGGGACGGATTTTTATCATCGGTATAAAGAAGAGACCGCCCTGATGGCAGAGATAGGATTCAAGGTTTTCCGGCCCTCAATTGCCGTGAGCCGTCTCTTTCCGCAGGGCGATGAAATCTCACCCGAGCAGCAGGGTATTGCTTGTTATCGTTCTGTCTTTGAAGAGTGTaaaaa                                                                                                                    <  2:64255/251‑1 (MQ=255)
                                                                                                                              ggCGGTGAAACTGGGGCTGGAAAAACGTTTTCAGTTGCGAGATGACGAGTTTTATCCCAGCCATGAGGCGACGGATTTTTATCATCGTTATAAAGAAGATATCGCCCTGATGGCAGAGATAGGATTCAAGGTTTTCCGTACCTCAATTGCCTGGAGCCGTCTCTTTCCGCAGGGCGATGAAATCACGCCCAATCAGCAGGGCATTGCTTTTTATCGTTCTGTCTTTGAAGAGTGTAAAAAGTACGGTATc                                                                                                          <  1:62507/250‑1 (MQ=255)
                                                                                                                                          ggggCTGGAAAAACGTTTTAAGTTGCGAGATGACGAGTTTTATCCCAGCCATGAGGCGACGGATTTTTATCATCGTTATAAAGAAGATATCGCCCTGATGGCAGAGATAGGATTCAAGGTTTTCCGTACCTCAATTGCCTGGAGCCGTCTCTTTCCGCAGGGCGATGAAATCACGCCCAATCAGCAGGGCATTGCTTTTTATCGTTCTGTCTTTGAAGAGTGTAAAAAGTACGGTATCGAACCGCTGGTc                                                                                              <  2:422269/250‑1 (MQ=255)
                                                                                                                                                 gAAAAACGTTTTCAGTTGCGAGATGACGAGTTTTATCCCAGCCATGAGGCGACGGATTTTTATCATCGTTATAAAGAAGATATCGCCCTGATGGCAGAGATAGGATTCAAGGTTTTCCGTACCTCAATTGCCTGGAGCCGTCTCTTTCCGCAGGGCGATGAAATCACGCCCAATCAGCAGGGCATTGCTTTTTATCGTTCTGTCTTTGAAGAGTGTAAAAAGTACGGTATCGAACCGCTGGTCACGTtgtg                                                                                      <  2:323945/251‑1 (MQ=255)
                                                                                                                                                                    gagaTGACGCGTTTTATCCCAGCCATGAGGCGACGGATTTTTATCATCGTTATAAAGAAGATATCGCCCTGATGGCAGAGATAGGATTCAAGGTTTTCCGTACCTCAATTGCCTGGAGCCGTCTCTTTCCGCAGGGCGATGAAATCACGCCCAATCAGCAGGGCATTGCTTTTTATCGTTCTGTCTTTGAAGAGTGTAAAAAGTACGGTATCGAACCGCTGGTCACGTTGTGCCACTTCGATGTGCCGATg                                                                   >  1:368696/1‑251 (MQ=255)
                                                                                                                                                                        tGACGAGTTTTATCCCAGCCATGAGGCGACGGATTTTTATCATCGTTATAAAGAAGATATCGCCCTGATGGCAGAGATAGGATTCAAGGTTTTCCGTACCTCAATTGCCTGGAGCCGTCTCTTTCCGCAGGGCGATGAAATCACGCCCAATCAGCAGGGCATTGCTTTTTATCGTTATGTCTTTGAAGAGTGTAAAAAGTACGGTATCGAACCGCTGGTCACGTTGTGCCACTTCGATGTGCCGATGCATc                                                               >  1:101117/1‑251 (MQ=255)
                                                                                                                                                                          aCGAGTTTTATCCCAGCCATGAGGCGACGGATTTTTATCATCGTTATAAAGAAGATATCGCCCTGATGGCAGAGATAGGATTCAAGGTTTTCCGTACCTCAATTGCCTGGAGCCGTCTCTTTCCGCAGGGCGATGAAATCACGCCCAATCAGCAGGGCATTGCTTTTTATCGTTCTGTCTTTGAAGAGTGTAAAAAGTACGGTATCGAACCGCTGGTCACGTTGTGCCACTTCGATGTGCCGATGCATCTg                                                             <  1:378201/251‑1 (MQ=255)
                                                                                                                                                                                     cccAGCCATGAGGCGACGGATTTTTATCATCGTTATAAAGAAGATATCGCCCTGATGGCAGAGATAGGATTCAAGGTTTTCCGTACCTCAATTGCCTGGAGCCGTCTCTTTCCGCAGGGCGATGAAATCACGCCCAATCAGCAGGGCATTGCTTTTTATCGTTCTGTCTTTGAAGAGTGTAAAAAGTACGGTATCGAACCGCTGGTCACGTTGTGCCACTTCGATGTGCCGATGCATCTGGTCACCGAat                                                   >  1:375549/1‑250 (MQ=255)
                                                                                                                                                                                             tGAGGCGACGGATTTTTATCATCGTTATAAAGAAGATATCGCCCTGATGGCAGAGATAGGATTCAAGGTTTTCCGTACCTCAATTGCCTGGAGCCGTCTCTTTCCGCAGGGCGATGAAATCACGCCCAATCAGCAGGGCATTGCTTTTTATCGTTCTGTCTTTGAAGAGTGTAAAAAGTACGGTATCGAACCGCTGGTCACGTTGTGCCACTTCGATGTGCCGATGCATCTGGTCACCGAATATGGCTCCt                                          >  1:123390/1‑251 (MQ=255)
                                                                                                                                                                                                       gATTTTTATCATCGTTATAAAGAAGATATCGCCCTGATGGCAGAGATAGGATTCAAGGTTTTCCGTACCTCAATTGCCTGGAGCCGTCTCTTTCCGCAGGGCGATGAAATCACGCCCAATCAGCAGGGCATTGCTTTTTATCGTTCTGTCTTTGAAGAGTGTAAAAAGTACGGTATCGAACCGCTGGTCACGTTGTGCCACTTCGATGTGCCGATGCATCTGGTCACCGAATATGGCTCCTGGCGTAACCg                                <  1:68748/251‑1 (MQ=255)
                                                                                                                                                                                                         tttttATCATCGTTATAAAGAAGATATCGCCCTGATGGCAGAGATAGGATTCAAAGTTTTCCGTACCTCAATTGCCTGGAGCCGTCTCTTTCCGCCGGGCGATGAAATCACGCCCAATCAGCAGGGCATTGCTTTTTATCGTTCTGTCTTTGAAGAGTGTAAAAAGAACGGTATCGAACCGCTGGTCACGTTTTGCCACTTCGATGTGCCGATGCATCTGGTCATCGAATATGGCTCCTGGCGTAACCGc                               >  1:254647/1‑250 (MQ=255)
                                                                                                                                                                                                          ttttATCATCGTTATAAAGAAGATATCGCCCTGATGGCAGAGATAGGATTCAAGGTTTTCCGTACCTCAATTGCCTGGAGCCGTCTCTTTCCGCAGGGCGATGAACTCACGCCCAATCAGCAGGGCATTGCTTTTTATCGTTCTGTCTTTGAAGAGTGTAAAAAGTACGGTATCGAACCGCTGGTCACGTTGTGCCACTTCGATGTGCCGATGCATCTGGTCACCGAATATGGCTCCTGGCGTAACCGCa                              >  2:457992/1‑250 (MQ=255)
                                                                                                                                                                                                                catcGTTATAAAGAAGATATCGCCCTGATGGCAGAGATAGGATTCAAGGTTTTCCGTACCTCAATTGCCTGGAGCCGTCTCTTTCCGCAGGGCGATGAAATCACGCCCAATCAGCAGGGCATTGCTTTTTATCGTTCTGTCTTTGAAGAGTGTAAAAAGTACGGTATCGAACCGCTGGTCACGTTGTGCCACTTCGATGTGCCGATGCATCTGGTCACCGAATATGGCTCCTGGCGTAACCGCAAGCtggg                       >  1:138450/1‑250 (MQ=255)
                                                                                                                                                                                                                                     gCCCTGATGGCAGAGATAGGATTCAAGGGTTTCCGTACCTCAATTGCCTGGAGCCGTCTCTTTCCGCAGGGCGATGAAAGCACGCCCAATCAGCAGGGCATTGCTTTTTATCGTTCTGGCTTTGAAGAGTGTAAAAAGTACGGTATCGAACCGCTGGTCACGTTGTGCCACTTCGATGTGCCGATGCATCTGGTCGCCGAATATGGCTCCTGGCGTAACCGCAAGCTGGTGGAGTTTTTCAGCCGCTACGc  <  1:42006/251‑1 (MQ=255)
                                                                                                                                                                                                                                            tGGCAGAGATAGGATTCAAGGTTTTCCGTACCTCAATTGCCTGGAGCCGTCTCTTTCCGCAGGGCGATGAAATCACGCCCAATCAGCAGGGCATTGCTTTTTATCGTTCTGTCTTTGAAGAGTGTAAAAAGTACGGTAt                                                                                                           >  1:378720/1‑139 (MQ=255)
                                                                                                                                                                                                                                            tGGCAGAGATAGGATTCAAGGTTTTCCGTACCTCAATTGCCTGGAGCCGTCTCTTTCCGCAGGGCGATGAAATCACGCCCAATCAGCAGGGCATTGCTTTTTATCGTTCTGTCTTTGAAGAGTGTAAAAAGTACGGTAt                                                                                                           <  2:378720/139‑1 (MQ=255)
                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                         
GTCAGTATTTCCAGAAAGTTTTTTATGGGGCGGCGCGCTTGCCGCCAACCAGTCTGAAGGTGCGTTCCGTGAAGGTGACAAAGGTCTGACCACTGTCGATATGATCCCACACGGCGAGCATCGAATGGCGGTGAAACTGGGGCTGGAAAAACGTTTTCAGTTGCGAGATGACGAGTTTTATCCCAGCCATGAGGCGACGGATTTTTATCATCGTTATAAAGAAGATATCGCCCTGATGGCAGAGATGGGATTCAAGGTTTTCCGTACCTCAATTGCCTGGAGCCGTCTCTTTCCGCAGGGCGATGAAATCACGCCCAATCAGCAGGGCATTGCTTTTTATCGTTCTGTCTTTGAAGAGTGTAAAAAGTACGGTATCGAACCGCTGGTCACGTTGTGCCACTTCGATGTGCCGATGCATCTGGTCACCGAATATGGCTCCTGGCGTAACCGCAAGCTGGTGGAGTTTTTCAGCCGCTACGC  >  NC_000913/2840992‑2841471

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑