Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,164,123	C→G	A162G (GCA→GGA)	btuB →	cobalamin outer membrane transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,164,123	0	C	G	93.0%	131.8 / ‑1.3	43	A162G (GCA→GGA)	btuB	cobalamin outer membrane transporter
Reads supporting (aligned to +/- strand):  ref base C (2/1);  new base G (16/24);  total (18/25)
Fisher's exact test for biased strand distribution p-value = 5.62e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

GTCAGCTCTCATCTATTTTTATTCGCGGTACAAATGCCAGTCATGTGTTGGTGTTAATTGATGGCGTACGCCTGAATCTGGCGGGGGTGAGTG‑GTTCTGCCGACCTTAGCCAGTTCCCTATTGCGCTTGTCCAGCGTGTTGAATATATCCGTGGGCCGCGCTCCGCTGTTTATGGTTCCGATGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTC‑AGCAGGGTGGGGAAGCAAT‑AGTT‑ATCAGAACTATGATGTCTCTACGCAGCAACAACTGGGGGATAAGACACGGGTAACGCTGTTGGGCGATTATGCCCATACTCATGGTTATGATGTTGTTGCCTATGGTAATACCGGAACGCAAGCGCAGACAGATAACGATGGTTTTTTAAGTAAAACGCTTTATGGCGCGCTGGAGCATAACTTTACTGATGCCTGGAGCGGCTTTGTG  >  NC_000913/4163880‑4164352                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                        gTCAGCTCTTATCTATTGTTATTCGCGGTACAAATGGCAGTCATGTGTTGGTGTTAGTTGATGGCGGTCGATTGATTTTGTCGGGGGTGAGTGTGTT‑TGCCGACCTTAGCCAGTTCCCTATTGCGCTTGTCCAGCGTGTTGAATATATCCGTGGGCCGCGCTCCGCTGTTGATGGTTCCGATGCAAGAGGAGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTC‑AGGAGGGTgg                                                                                                                                                                                                                                  <  2:102830/251‑1 (MQ=255)           atttagttttATTCGCGGTACAAATGCCAGTCATGTGTTGGTGTTAATTGATGGCGTACGCCTGAAGCTGGCGGGGGTGAGTG‑GTTCTGCCGCCCTTAGCCAGTTCCCTATTGCGCTTGTCCAGCGTGTTGAATATATCCGTGGGCCGCGCTCCGCTGTTTATTGTTCCGATGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTC‑AGGAGGGTTGGGAAGCAAT‑a                                                                                                                                                                                                                       <  1:464847/245‑1 (MQ=255)                               cAAATGCCAGTCATGTGTTGGTGTTAATTGATGGCGTACGCCTGAATCTGGCGGGGGTGAGTG‑GTTCTGCCGACCTTAGCAAGTTCCCTATTGCGCTTGTCCAGCGTGTTGAATATATCCGTGGGCCGCGCTCCGCTGTTTATGGTTCCGATGCAATAGGCGGGGGGGGGAATTTCATCACGACGCGCGATGAACCCGGGACGGAAAATTC‑AGGAGGGGGGGGAAGCAAT‑AATT‑ATCAAAAATATGATGtt                                                                                                                                                                                                  >  2:20579/1‑250 (MQ=255)                                 aaTGCCAGGCATGTGTTGGGGTTAATTGATGGCGTACGCCTGAATCTGGCGGGGGTGAGTG‑GTTCTGCCGACCTTAGCCAGTTCCCTATTGCGCTTGTCCAGCGTGTTGAATATATCCGTGGGCCGCGCTCCGCTGTTTCTGGTTCCGATGCCATAGTCGGGGTGGTGAATATCATCCCGACGCGCGATGAACCCGGACCGGAAATTTA‑ATCCGGGGGGGGAAACAATTAGT‑‑CTCAGAACTCTGATGtctc                                                                                                                                                                                                >  2:35663/1‑251 (MQ=255)                                       agtcctgggtTGGTGTTAATTGATGGCGTACGCCTGAATCTGGCGGGGGTGAGTG‑GTTCTGCCGACCTTAGCCAGTTCCCTATTGCGCTTGTCCAGCGTGTTGAATATATCCGTGGGCCGCGCTCCGCTGTTTATGGTTCCGATGCAATTGGCGGGGTGGTGAATGTCAACCCACCGCGCGGAGAACCCGGAACGGGAATTTCCTGGAGG‑TGGGGAAGCAAT‑AGTTTACCA‑AACTAAGATGTCTCTACgcc                                                                                                                                                                                          >  2:160468/9‑250 (MQ=255)                                       aGTCATGTGTTGGTGTTAATTGATGGCGTACGCCTGAATCTGGCGGGGGTGAGTG‑GTTCTGCCGACCTTAGCCAGTTCCCTATTGCGCTTGTCCAGCGTGTTGAATATATCCGTGGGCCGCGCTCCGCTGTTTATGGTTCCGATGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTC‑AGGAGGGTGGGGAAGCAAT‑AGTT‑ATCAGAACTATGATGTCTCTACgca                                                                                                                                                                                          <  1:242375/251‑1 (MQ=255)                                                         aTTGATGGCGTACGCCTGAATCTGGCGGGGGTGAGTG‑GTTCTGCCGACCTTAGCCAGTTCCCTATTGCGCTTGTCCAGCGTGTTGAATATATCCGTGGGCCGCGCTCCGCTGTTTATGGTTCCGATGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTC‑AGGAGGGTGGGGAAGCAAT‑AGTT‑ATCAGAACTATGATGTCTCTACGCAGCAACAACTGGGGGATa                                                                                                                                                                         >  1:395425/1‑250 (MQ=255)                                                           tGATGGCGTACGCCTGAATCTGGCGGGGGTGAGTG‑GTTCTGCCGACCTTAGCCAGTTCCCTATTGCGCTTGTCCAGCGTGTTGAATATATCCGTGGGCCGCGCTCCGCTGTTTATGGTTCCGATGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTT‑AGGGGGGGGGGGAAGCAAA‑GGTT‑ATCAGAACTAGGATGTCACTACGCAGCAACAACTGGGGGATa                                                                                                                                                                         >  1:341158/1‑248 (MQ=255)                                                                       ccTGAATCTGGCGGGGGTGAGTG‑GTTCTGCCGTCCTTAGCCAGTTCCCTATTGCGTGTGTCCAGGGTGTTGAATATATCCGTGGGCCGCGCTCCGCTGTTTATGGTTCCGATGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTC‑AGGAGGGTGGGGAAGCAAT‑AGTT‑ATCAGAACTATGAAGTCTCTACGCAGCAACAACTGGGGGATAAGACACGGGTAACGc                                                                                                                                                          <  2:138154/251‑1 (MQ=255)                                                                        cTGAATCTGGCGGGGGTGAGTG‑GTTCTGCCGACCTTAGCCAGTTCCCTATTGCGCTTGTCCAGCGTGTTGAATATATCCGTGGGCCGCGCTCCGCTGTTTATGGTTCCGATGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTC‑AGGAGGGTGGGGAAGCAAT‑AGTT‑ATCAGAACTATGATGTCTCTACGCAGCAACAACTggggg                                                                                                                                                                            >  1:193905/1‑232 (MQ=255)                                                                        cTGAATCTGGCGGGGGTGAGTG‑GTTCTGCCGACCTTAGCCAGTTCCCTATTGCGCTTGTCCAGCGTGTTGAATATATCCGTGGGCCGCGCTCCGCTGTTTATGGTTCCGATGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTC‑AGGAGGGTGGGGAAGCAAT‑AGTT‑ATCAGAACTATGATGTCTCTACGCAGCAACAACTggggg                                                                                                                                                                            <  2:193905/232‑1 (MQ=255)                                                                          taaTCTGGCGGGGGTGAGTG‑GTTCTGCCGACCTTAGCCAGTTCCCTATTGCGCTTGTCCAGCGTGTTGAATATATCCGTGGGCCGCGCTCCGCTGTTTATGGTTCCGATGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATGTC‑AGGAGGGTGGGGAAGCAAT‑AGTT‑ATCAGAACTATGATGTCTCTACGCAGCAACAACTGGGGGATAAGACACGGGTAACGCTg                                                                                                                                                        <  2:40186/249‑1 (MQ=255)                                                                                    ggggTGAGTG‑GTTCTGCCGACCTTAGCCAGTTCCCTATTGCGCTTGTCCAGCGTGTTGAATATATCCGTGGGCCGCGCTCCGCTGTTTATGGTTCCGATGCAATAGGCGGGGTGGGGAATATCATCACGACGCGCGATGAACCCGGAACGGCAATTTC‑AGGAGGGTGGGGAAGCAAT‑AGTT‑ATCAGAACTatgctg                                                                                                                                                                                                    >  1:307571/1‑193 (MQ=255)                                                                                    ggggTGAGTG‑GTTCTGCCGACCTTAGCCAGTTCCCTATTGCGCTTGTCCAGCGTGTTGAATATATCCGTGGGCCGCGCTCCGCTGTTTATGGTGCCGATGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACTGAAATTTC‑AGGAGGTTGGGGAAGCAAT‑AGTT‑ATCAGAACTatgatg                                                                                                                                                                                                    <  2:307571/196‑1 (MQ=255)                                                                                     gggTGAGTG‑GTTCTGCCGATCTTAGCCAGTTCCCTATTGCGCTTGTCCAGCGTGTTGAATATATCCGTGGGCCGCGCTCCGCTGTTTATGGTTCCGATGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTC‑AGGAGGGTGGGGAAGCAAT‑AGTT‑ATCAGAACTATGATGTCTCTACGCAGCAACAACTGGGGGATAAGACACGGGTAACGCTGTTGGGCGATTAt                                                                                                                                            <  1:352795/251‑1 (MQ=255)                                                                                      ggTGAGTG‑GTTCTGCCGACCTTAGCCAGTTCCCTATTGCGCTTGTCCAGCGTGTTGAATATATCCGTGGGCCGCGCTCCGCTGTTTATGGTTCCGATGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTC‑AGGAGGGTGGGGAAGCAAT‑AGTT‑ATCAGAACTATGATGTCTCTACGCAGCAACAACTGGGGGATAAGACACGGGTAACGCTGTTGGGCGATTATg                                                                                                                                           <  1:172140/251‑1 (MQ=255)                                                                                           atG‑GTTCTGCCGACCTTAGCCAGTTCCCTATTGCGCTTGACCAGCGTGATGAATATATCCGTGGGCCGCGCTCAGCTGTTTATGGTTCCGATGCCATAGGCGGGGTGGAGAATATCCTAACGACGCGCGATGAACCCGGAACGGCACTTTC‑AGGCGGGTGGGGAAGCCAT‑AGAT‑ATCAGCACCATGACGGCTTTATGCAGCCGCAAATGGGGGATAATACACGCGGAACGCTGTTGGGCGATTATGCCCac                                                                                                                                      >  2:318534/2‑250 (MQ=255)                                                                                               gTTCTGCCGACCTTAGCCAGTTCCCTATTGCACTTGTCCAGCGTGTTGAATATATCCGTGGGCCGCGCTCCGCTGTTTATGGTTCCGATGCAATAGGCGGGGTGGTGAATATCATCCAGACGCGCGATGAAACCGGAACGGAAATTTC‑AGGAGGGAGGGGAAGCCAT‑AGTT‑ATCAGAACTATGATGTCTCTACGCAGCAACAACTGGGGGATAAGACACGGGTAAAGATATTGGGGGTTTATGCCCATACt                                                                                                                                   >  1:168144/1‑251 (MQ=255)                                                                                                         ccTTAGCCCGTTCCCTATTGCGCTTGTCCAGCGTGTTGAATATATCCGTGGGCCGCGCTCCGCTGTTTATGGTTCCGATGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTC‑AGGAGGGTGGGGAAGCAAT‑AGTT‑ATCAGAACTATGATGTCTCTACGCAGCAACAACTGGGGGATAAGACACGGGTAACGCTGTTGGGCGATTATGCCCATACTCATGGTTatg                                                                                                                         <  1:45643/251‑1 (MQ=255)                                                                                                                     cccTATTGCGCTTGTCCAGCGTGTTGAATATATCCGTGGGCCGCGCTCCGCTGTTTATGGTTCCGATGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTC‑AGGAGGGTGGGGAAGCAAT‑AGTT‑ATCAGAACTATGATGTCTCTACGCAGCAACAACTGGGGGATAAGACACGGGTAACGCTGTTGGGCGATTATGCCCATACTCATGGTTATGATGTTGTTGCCt                                                                                                             <  1:464966/251‑1 (MQ=255)                                                                                                                            gcgcTTGTCCAGCGTGTTGAATATATCCGTGGGCCGCGCTCCGCTGTTTATGGTTCCGATGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTC‑AGCAGGGTGGGGAAGCAAT‑AGTT‑ATCAGAACTATGATGTCTCTACGCAGCAACAACTGGGGGATAAGACACGGGTAACGCTGTTGGGCGATTATGCCCATACTCATGGTTATGATGTTGTTGCCTATGGTa                                                                                                       <  1:35663/250‑1 (MQ=255)                                                                                                                                    ccAGCGTGTTGAATATATCCGTGGGCCGCGCTCCGCTGTTTATGGTTCCGAGGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTC‑AGGAGGGTGGGGAAGCAAT‑AGTT‑ATCAGAACTATGATGTCTCTACGCAGCAACAACTGGGGGATAAGACACGGGTAACGCTGTTGGGCGATTATGCCCATACTCATGGTTATGATGTTGTTGCCTATGGTAATACCGGa                                                                                               <  1:160468/250‑1 (MQ=255)                                                                                                                                       gCGTGTTGAATATATCCGTGGGCCGCGCTCCGCTGTTTATGGTTCCGATGCAATAGGCGGGGGGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTC‑AGGAGGGTGGGGAAGCAAT‑AGTT‑ATCAGAAATATGATGTCTCTACGCAGCAACAACTGGGGGATAAGACACGGGTAACGCTGTTGGGCGATTATGCCCATACTAATGGGTATGATGTTGTTGCCTATGGTAATACCGGAACGc                                                                                           >  1:245322/1‑251 (MQ=255)                                                                                                                                        ggtgtTGAAGATATCCGTGGGCCGCGCTCCGCTGTTTATGGTTCCGATGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTC‑AGGAGGGTGGGGAAGCAAT‑AGTT‑ATCAGAACTATGATGTCTCTACGCAGCAACAACTGGTGGATAAGACACGGGTAACGCTGTTGGGCGATTATGCCCATCCTCATGGTTATGATGTTGTTGCCTATGGTAATACCGGatctct                                                                                          <  2:75826/250‑6 (MQ=255)                                                                                                                                           gtTGAATATATCCGTGGGCCGCGCTTCGTTGTTTATTGTTCCGATGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTC‑AGGAGGGTGGGGAAGCAAT‑AGTT‑ATCAGAACTATGATGTCTCTACGCAGCAACAACTGGGGGATAAGACACGGGTAACGCTGTTGGGCGATTATGCCCATACTCATGGTTATGATGTTGTTGCCGATGGTAATAACGGAACGCAAgc                                                                                       <  2:90278/251‑1 (MQ=255)                                                                                                                                               aaTATATCCGTGGGCCGCGCTCCTCTGTTTATGGTTCCGATGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTC‑AGGAGGGTGGGGAAGCAAT‑AGTT‑ATCAGAACTATGATGTCTCTACGCAGCAACAACTGGGGGATAAGACACGGGTAACGCTGTTGGGCGATTATGCCCATACTCATGGTTATGATGTTGTTGCCTATGGTAATACCGGAACGCAAGCGcag                                                                                   <  2:106834/251‑1 (MQ=255)                                                                                                                                                      ccGTGGGCCGCGCTCCGCTGTTTATGGTTCCGATGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTC‑AGGAGGGTGGGGAAGCAAT‑AGTT‑ATCAGAACTATGATGTCTCTACGCAGCAACAACTGGGGGATAAGACACGGGTAACGCTGTTGGGCGATTATGCCCGTACCCATGGTTTTGATGTTGTTGCCTCTGGTAATACCGGGACGCAAGCGcagacaaat                                                                             >  2:174164/1‑247 (MQ=255)                                                                                                                                                      ccGTGGGCCGCGCTCCGCTGTTTATGGTTCCGATGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTC‑AGGAGGGTGGGGAAGCAAT‑AGTT‑ATCAGAACTATGATGTCTCTACGCAGCAACAACTGGGGGATAAGACACGGGTAACGCTGTTGGGCGATTATGCCCATACTCATGGTTATGATGTTGTTGCCTATGGTAATACCGGAACGCAAGCGCAGACAGAt                                                                             <  1:420697/250‑1 (MQ=255)                                                                                                                                                            gCCGCGCTCCGCTGTTTATGGTTCCGATGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTGC‑AGGAGGGTGGGGAAGCAAT‑AGTT‑ATCAGAACTATGATGTCTCTACGCAGCAACAACTGGGGGATAAGACACGGGTAACGCTGTTGGGCGATTATGCCCATACTCATGGTTATGATGTTGTTGCCTATGGTAATACCGGAACGCAAGCGCAGACAGATAACGATg                                                                      <  1:158340/251‑1 (MQ=255)                                                                                                                                                                     cGCTGTTTATGGTTCCGATGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTC‑AGGAGGGTGGGGAAGCAAT‑AGTT‑ATCAGAACTATGATGTCTCTACGCAGCAACAACTGGGGGATAAGACACGGGTAACGCTGTTGGGCGATTATGCCCATACTCATGGTTATGATGTTGTTGCCTATGGTAATACCGGAACGCAAGCGCAGACAGATAACGATGGTTTTTTa                                                              >  1:363203/1‑250 (MQ=255)                                                                                                                                                                           ttATGGTTCCGATGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTC‑AGGAGGGTGGGGAAGCAAT‑AGTT‑ATCAGAACTATGATGTCTCTACGCAGCAACAACTGGGGGATAAGACACGGGTAACGCTGTTGGGCGATTATGCCCATACTCATGGTTATGATGTTGTTGCCTATGGTAATACCGGAACGCAAGCGCAGACAGATAACGATGGTTTTTTAAGTaaa                                                        <  1:401188/250‑1 (MQ=255)                                                                                                                                                                              gggTTCCGATGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTC‑AGGAGGGTGGGGAAGCAAT‑AGTT‑ATCAGAACTATGATGTCTCTACGCAGCAACAACTGGGGGATAAGACACGGGTAACGCTGTTGGGCGATTATGCCCATACTCATGGTTATGATGTTGTTGCCTATGGTAATACCGGAACGCAAGCGCAGACAGATAACGATGGTTTTTTAAGTAAAACGc                                                    <  1:278483/250‑1 (MQ=255)                                                                                                                                                                                      aagaaaaaGGCGGGGTGGGGAATATCATCACGACGCGCGATGAACCCGGAACGGAAAGTTC‑ATGAGGTTGGGGAAGCAAT‑AGGT‑ATCAGAACTATGATGTCTCTACGCAGCAACAACTGGGGGATAAGACACGGGTAACGCTGTTGGGCGATTATGCCCATACTCATGGTTATGATGTTGTTGCCTATGGTAAGACCGGAACGCAAGCGCAGACAGATAACGATGGTTTTTTAAGTAAAACGCTTTATGgc                                            <  2:17263/244‑1 (MQ=255)                                                                                                                                                                                      aTGCAGTAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTC‑AGCAGGGTGGGGAAGCAAT‑AGTT‑ATCAGAACTATGATGTCTCTACGCAGCAACAACTGGGGGATAAGACACGGGTAACGCTGTCGGGCGATTATGCCCATACTCATGGTTATGATGTTGTTGCCTATGGTAATACCGGAACGCAAGCGCAGACAGATAACGATGGTTTTTTAAGTAAAACGCTTTATGgc                                            >  2:319596/1‑251 (MQ=255)                                                                                                                                                                                             aGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTC‑AGGAGGGTGGGGAAGCAAT‑AGTT‑ATCAGAACTATGATGTCTCTACGCAGCAACAACTGGGGGATAAGACACGGGTAACGCTGTTGGGCGATTATGCCCATACTCATGGTTATGATGTTGTTGCCTATGGTAATACCGGAACGCAAGCGCAGACAGATAACGATGGTTTTTTAAGTAAAACGCTTTATGGCGCGCTgg                                     >  1:272207/1‑251 (MQ=255)                                                                                                                                                                                                gggggTTGTGAAGATCATCACGACGCGCGATGAACCCGGAACGGAAATTTC‑AGGAGGGTGGGGAAGCAAT‑AGGT‑ATCAGAACTATGATTGCTCTACGCTGCAACAACGGGGGGATAAGACACGGGTAACGCTGTTGGGCGAGTATGCCCATACTCATGGTTACGATGTTGTTGTCTATGGTAATACCGGAACGCAAGCGCAGACAGATAACGATGGTTTGTTAAGTAAAACGCTTTATGTCGCGCTGGAGc                                  <  2:95101/250‑1 (MQ=255)                                                                                                                                                                                                           atatCATCACGACGCGCGATGAACCCGGCACGGAAATTTC‑AGGAGGGTGGGGCAGCAAT‑AGTT‑ATCAGAACTATGATGTCTCTACGCAGCAACAACTGGGGGATAAGACACGGGTAACGCTGTTGGGCGATTATGCCCATACTCATGGTTATGCTGTTGTTGCCTATGGTAATACCGGAACGCAAGCGCAGACAGATAACGATGGTTTTTTAAGTAAAACGCTTTATGGCGCGCTGGAGCATAACTTTACt                       >  2:378322/1‑251 (MQ=255)                                                                                                                                                                                                            tatCATCACGACGCGCGATGAACCCGGAACGGAAATTTC‑AGGAGGGTGGGGAAGCAAT‑AGTT‑ATCAGAACTATGATGTCTCTACGCAGCAACAACTGGGGGATAAGACACGGGTAACGCTGTTGGGCGATTATGCCCATACTCATGGTTATGATGTTGTTGCCTATGGTAATACCGGAACGCAAGCGAAGACAGAAAACGATGGTTTTTTAAGTAAAACGCTTTATGgc                                            >  1:320021/1‑229 (MQ=255)                                                                                                                                                                                                            tatCATCACGACGCGCGAGGAACCCGGAACGGAAATTTC‑AGGAGGGTGGGGAAGCAAT‑AGTG‑ATAAGAACTATGATGTCTCTACGCAGCAACAACTGGGGGATAAGACACGGGTAACGTTGTTGGGCGATTATGCCCATACTCATGGTTATGATGTTGTTGCCTATGGTAATACCGGAACGCAAGCGCAGACAGATAACGATGGTTTTTTAAGTAAAACGCTTTATGgc                                            <  2:320021/229‑1 (MQ=255)                                                                                                                                                                                                                   acgacgCGCGATGAACCCGGAACGGAAATTTC‑AGGAGGGTGGGGAAGCAAT‑AGTT‑ATCAGAACTATGATGTCTCTACGCAGCAACAACTGGGGGATAAGACACGGGTAACGCTGTTGGGCGATTATGCCCATACTCATGGTTATGATGTTGTTGCCTATGGTAATACCGGAACGCAAGCGCAGACAGATAACGATGGTTTTTTAAGTAAAACGCTTTATGGCGCGCTGGAGCATAACTTTACTGAGGCCTg               >  2:203711/1‑251 (MQ=255)                                                                                                                                                                                                                     gacgCGCGATGAACCCGGAAAGGAAATTTC‑AGGAGGGTGGGGAAGCAAT‑AGTT‑AGAAGAACTATGATGTCTCGACGCAGCAACAACGGGGGGATAAGACACGGGTAACGCTGTTGGGCGATTATGCTCATACTCATGGTTATGATGTTGTTGCCTATGGTAATACCGGAACGCAAGCGCAGACAGATAACGATTGTTTTTTAAGTAAAACGATTTATGGCGCGCTGGAGTATAACTTTACTGATGCCTGGa             <  2:103004/251‑1 (MQ=255)                                                                                                                                                                                                                             aTGAACCCGGAACGGAAATGTC‑AGGAGGGTGGGGAAGCAAT‑AGTT‑ATCAGAACTATGATGTCTCTACGCAGCAACAACTGGGGGATAAGACACGGGTAACGCTGTTGGGCGATTATGCCCATACTCATGGGTATGATGTTGTTGCCTATGGTAATACCGGAACGCAAGCGCAGACAGATAACGATGGTTTTTTAAGTAAAACGCTTTATGGCGCGCTGGAGCATAACTTTACTGATGCCTGGAGCGGCttt     <  1:288739/251‑1 (MQ=255)                                                                                                                                                                                                                                aaCCCGGAACGGAAATTTC‑AGGAGGGTGGGGAAGCAAT‑AGTT‑ATCAGAACTATGATGTCTCTACGCAGCAACAACTGGGGGATAAGACACGGGTAACGCTGTTGGGCGATTATGCCCATACTCATGGTTATGATGTTGTTGCCTATGGTAATACCGGAACGCAAGCGCAGACAGATAACGATGGTTTTTTAAGTAAAACGCTTTATGGCGCGCTGGAGCATAACTTTACTGATGCCTGGAGCGGCTTtgtg  >  1:190751/1‑251 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                        GTCAGCTCTCATCTATTTTTATTCGCGGTACAAATGCCAGTCATGTGTTGGTGTTAATTGATGGCGTACGCCTGAATCTGGCGGGGGTGAGTG‑GTTCTGCCGACCTTAGCCAGTTCCCTATTGCGCTTGTCCAGCGTGTTGAATATATCCGTGGGCCGCGCTCCGCTGTTTATGGTTCCGATGCAATAGGCGGGGTGGTGAATATCATCACGACGCGCGATGAACCCGGAACGGAAATTTC‑AGCAGGGTGGGGAAGCAAT‑AGTT‑ATCAGAACTATGATGTCTCTACGCAGCAACAACTGGGGGATAAGACACGGGTAACGCTGTTGGGCGATTATGCCCATACTCATGGTTATGATGTTGTTGCCTATGGTAATACCGGAACGCAAGCGCAGACAGATAACGATGGTTTTTTAAGTAAAACGCTTTATGGCGCGCTGGAGCATAACTTTACTGATGCCTGGAGCGGCTTTGTG  >  NC_000913/4163880‑4164352

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑