Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	85,481	T→C	R11G (AGA→GGA)	yabR ←	protein YabR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	85,481	0	T	C	100.0%	89.3 / NA	27	R11G (AGA→GGA)	yabR	protein YabR
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (11/16);  total (11/16)

AGCTGCCGGGCGCGATAAAGGCCATCAGTGGATGGAAGAGCAATTAGTCTCAATTTGCAAACGCTAACTGATTGCAGAATAGGTCAGACATGAATGTCTGGTTTATTCTGCATTTTTTATTGAATGTAGAATTTTATTCTGAATGTGTGGGCTCTCTATTTTAGGATTAATTAAAAAAATAGAGAAATTGCTGTAAGTTGTGGGATTCAGCCGATTTATTATCAATTTAATCCTCTGTAATGGAGGATTTTATCGTTTCTTTTCACCTTTCCTCCTGTTTATTCTTATTACCCCGTGTTTATGTCTCTGGCTGCCAATTGCTTAAGCAAGATCGGACGGTTAATGTGTTTTACACATTTTTTCCGTCAAACAGTGAGGCAGGCCATGGAGATGTTGTCTGGAGCCGAGATGGTCGTCCGATCGCTTATCGATCAGGGCGTTAAACAAGTATTCGGTTATCCCGGAGGCGCAGTCCTTGAT  >  NC_000913/85246‑85725                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                     aGCTGCCGGGCGCGATAAAGGCCATCAGTGGCTGGAAGAGCAATTAGTCTCAATTTGCAAACGCTAACTGATTGCAGAATAGGTCAGACATGAATGTCTGGTTTATTCTGCATTTTTTATTGAATGTAGAATTTTATTCTGAATGTGTGGGCTCTCTATTTTAGGATTAATTAAAAAAATAGAGAAATTGCTGTAAGTTGTGGGATTCAGCCGATTTATTATCAATTTAATCCTCCGTAATGGAGGAtttt                                                                                                                                                                                                                                       >  1:367164/1‑251 (MQ=255)         ggcctgATAAAGTCCATCAGTGGAAGGAAGAGCAATTAGTCTCAATTTGCAAACGCTAACTGATTGCAGAATAGGTCAGACATGAATTTCTGGTTTATTCTGCATTTTTTATTGAATGTAGAATTTTATTCTGAATGTGTGGGCTCTCTATTTTAGGATTAATTAAAAAAATAGAGAAATTGCTGTAAGTTGTGGGATTCAGCCGATTTATTATCAATTTAATCCTCCGTAATGGAGGATGTTATCGTTTc                                                                                                                                                                                                                               <  2:104918/246‑1 (MQ=255)          gcgcGATAAAGGCCATCAGTGGATGGAAGAGCAATTAGTCTCAATTTGCAAACGCTAACTGATTGCAGAATAGGTCAGATATGAATGTTTGGTTTATTCTGCATTTTTTATTGAATGTAGAATTTTATTCTGAATGTGTGGGCTCTCTATTTTAGGATTAATTAAAAAAATAGAGAAATTGCGGTAAGTTGTGGGATTCAGCCGAGTTATTATCAATTTAATCCTCCGTAATGGAGGATTTTATCGTTTCt                                                                                                                                                                                                                              <  2:386398/251‑1 (MQ=255)                                           aTTAGTCTCAATTTGCAAACGCTAACTGATTGCAGAATAGGTCAGACATGAATGTCTGGTTTATTCTGCATTTTTTATTGAATGTAGAATTTTATTCTGAATGTGTGGGCTCTCTATTTTAGGATTAATTAAAAAAATAGAGAAATTGCTGTAAGTTGTGGGATTCAGCCGATTTATTATCAATTTAATCCTCCGTAATGGAGGATTTTATCGTTTCTTTTCACCTTTCCTCCTGTTTATTCTTATTAccc                                                                                                                                                                                             >  1:149612/1‑251 (MQ=255)                                               gTCTCAATTTGCAAACGCTAACTGATTGCAGAATAGGTCAGACATGAATGTCTGGTTTATTCTGCATTTTTTATTGAATGTAGAATTTTATTCTGAATGTGTGGGCTCTCTATTTTAGGATTAATTAAAAAAATAGAGAAATTGCTGTAAGTTGTGGGATTCAGCCGATTTATTATCAATTTAATCCTCCGTAATGGAGGATTTTATCGTTTCTTTTCACCTTTCCTCCTGTTTATTCTTATTACCCCgtg                                                                                                                                                                                         <  1:360282/251‑1 (MQ=255)                                                                 tAACTGATTGCAGAATAGGTCAGACATGAATGTCTGGTTTATTCTGCATTTTTTATTGAATGTAGAATTTTATTCTGAATGTGTGGGCTCTCTATTTTAGGATTAATTAAAAAAATAGAGAAATTGCTGTAAGTTGTGGGATTCAGCCGATTTATTATCAATTTAATCCTCCGTAATGGAGGATTTTATCGTTTCTTTTCACCTTTCCTCCTGTTTATTCTTATTACCACGTGTTTATGTCTCTGTCTGcc                                                                                                                                                                       >  1:18769/1‑251 (MQ=255)                                                                         tGCAGAAGAGGTCAGACATGAATGTCTGGTTTATTCTGCATTTTATATTGAATGTAGAATTGTAGGCTGAATTTGTGGGCTCTCTAGTTTAGGATTAATTAAAAAAATAGAGAAATTGCTGTAAGTTGTGGGATTCAGCCGATTTATTATCAAGTTAATCCGCCGTAATGGAGGATTTTATCGTTTCCTTGCGCCTTTCCTCCTGTGTATACTTATTACCCCTTGTTTATGTCGCTGGCTGCGAATTGCt                                                                                                                                                                <  2:42373/250‑1 (MQ=255)                                                                                                     gTTTATTCTGCATTTTTTATTGAATGTAGAATTTTATTCTGAATGTGTGGGCTCTCTATTTTAGGATTAATTAAAAAAATAGAGAAATTGCTGTAAGTTGTGGGATTCAGCCGATTTATTATCAATTTAATCCTCCGTAATGGAGGATTTTATCGTTTCTTTTCACCTTTCCTCCTGTTTATTCTTATTACCCCGTGTTTATGTCTCTGGCTGCCAATTGCTTAAGCAAGATCGGACGGTTAATGTGtttt                                                                                                                                   <  1:140178/251‑1 (MQ=255)                                                                                                                       aTTGAATGTAGAATTTTATTCTGAATGTGTGGGCTCTCTATTTTAGGATTAATTAAAAAAATAGAGAAATTGCTGTAAGTTGTGGGATTCAGCCGATTTATTATCAATTTAATCCTCCGTAATGGAGGATTTTATCGTTTCTTTTCACCTTTCCTCCTGTTTATTCTTATTACCCCGTGTTTATGTCTCTGGCTGCCAATTGCTTAAGCAAGATCGGACGGTTAATGTGTTTTACACATTTTTTCCGTCa                                                                                                                  <  1:123736/250‑1 (MQ=255)                                                                                                                              gTAGAATTTTATTCTGAATGTGTGGGCTCTCTATTTTAGGATTAATTAAAAAAATAGAGAAATTGCTGTAAGTTGTGGGATTCAGCCGATTTATTATCAATTTAATCCTCCGTAATGGAGGATTTTATCGTTTCTTTTCACCTTTCCTCCTGTTTATTCTTATTACCCCGTGTTTATGTCTCTGGCTGCCAATTGCTTAAGCAAGATCGGACGGTTAATGTGTTTTACACATTTTTTCCGTCAAACAGTg                                                                                                           <  1:305869/250‑1 (MQ=255)                                                                                                                                             taaTGTGTGGGCTCTATATTTTAGGATTAATTAAAAAAATAGAGAAATTGCTGTAAGTTGTGGGATTCAGCCGATTTATTATCAATTTAATCATCCGTAATGGAGGATTTTATCGTTTCTTTTCACCTTTCCTCCTGTTTATTCTTATTACCCCGTGTTTATGTCTCTGGCTGCCAATTGCTTAAGCAAGATCGGACGGTTAATGTGTTTTACACATTTTTTCCGTCAAACAGTGAGGCAGGCCATgg                                                                                              >  1:245544/2‑248 (MQ=255)                                                                                                                                                               ttttAGGATTAATTAAAAAAATAGAGAAATTGCTGTAAGTTGTGGGATTCAGCCGATTTATTATCAATTTAATCCTCCGTAATGGAGGATTTTATCGTTTCTTTTCACCTTTCCTCCTGTTTATTCTTATTACCCCGTGTTTATGTCTCTGGCTGCCAATTGCTTAAGCAAGATCGGACGGTTAATGTGTTTTACACATTTTTTCCGTCAAACAGTGAGGCAGGCCATGGAGATGTTGTCTGGAGCCg                                                                            >  1:288330/1‑248 (MQ=255)                                                                                                                                                                   aGGATTAATTAAAAAAATAGAGAAATTGCTGTAAGTTGTGGGATTCAGCCGATTTATTATCAATTTAATCCTCCGTAATGGAGGATTTTATCGTTTCTTTTCACCTTTCCTCCTGTTTATTCTTATTACCCCGTGTTTATGTCTCTGGCTGCCAATTGCTTAAGCAAGATCGGACGGTTAATGTGTTTTACACATTTTTTCCGTCAAACAGTGAGGCAGGCCATGGAGATGTTGTCTGGAGCCGAGATgg                                                                      <  2:395633/250‑1 (MQ=255)                                                                                                                                                                   aGGATTAATTAAAAAAATAGAGAAATTGCTGTAAGTTGTGGGATTCAGCCGATTTATTATCAATTTAATCCTCCGTAATGGAGGATTTTATCGTTTCTTTTCACCTTTCCTCCTGTTTATTCTTATTACCCCGTGTTTATGTCTCTGGCTGCCAATTGCTTAAGCAAGATCGGACGGTTAATGTGTTTTACACATTTTTTCCGTCAAACAGTGAGGCAGGCCATGGAGATGTTGTCTGGAGCCGAGATGgt                                                                     <  2:368833/251‑1 (MQ=255)                                                                                                                                                                               aaaaaTAGAGAAATTGCTGTAAGTTGTGGGATTCAGCCGAATTATTATCAATTTAATCCTCCGTAATGGAGGATTTTATCGTTTCTTTTCACCTTTCCTCCTGTTTATTCTTATTACCCCGTGTTTATGTCTCTGGCTGCCAATTGCTTAAGCAAGATCGGACGGTTAATGTGTTTTACACATTTTTTCCGTCAAACAGTGAGGCAGGCCATGGAGATGTTGTCTGGAGCCGCGATGGTCGTCCGATCGCt                                                         >  1:419207/1‑251 (MQ=255)                                                                                                                                                                                 aaaTAGAGAAATTGCTGTAAGTTGTGGGATTCAGCCGATTTATTATCAATTTAATCCTCCGTAATGGAGGATTTTATCGTTTCTTTTCACCTTTCCTCCTGTTTATTCTTATTACCCCGTGTTTATGTCTCTGGCTGCCAATTGCTTAAGCAAGATCGGACGGTTAATGTGTTTTACACATTTTTTCCGTCAAACAGTGAGGCAGGCCATGGAGATGTTGTCTGGAGCCGAGATGGTCGTCCGATCGCtt                                                        >  1:250476/1‑250 (MQ=255)                                                                                                                                                                                        gaAATTGCTGTAAGTTGTGGGATTCAGCCGATTTATTATCAATTTAATCCTCCGTAATGGAGGATTTTATCGTTTCTTTTCACCTTTCCTCCTGTTTATTCTTATTACCCCGTGTTTATGTCTCTGGCTGCCAATTGCTTAAGCAAGATCGGACGGTTAATGTGTTTTACACATTTTTTCCGTCAAACAGTGAGGCAGGCCATGGAGATGTTGTCTGGAGCCGAGATGGTCGTCCGATCGCTTATCGATc                                                 >  1:333295/1‑250 (MQ=255)                                                                                                                                                                                         aaaTTGCTGTAAGGTGTGGGATTCAGCCGAGTTATTATCAATTGAATCCTCCGTAATGGAGGATTTTATCGTTTCTTGTCACCTTTCCTCCTGTTTATTCTTATTACCCCGCGTTTATGTCTCTGGCTGCCAATTGCTTAAGCAAGATCGGACGGTTAATGTGTTTTACACATTTTTTCCGGCAAACAGTGAGGCAGGCCATGGAGATGTTGTGTGGAGCCGCGATGGTCGTCCGATCGCTTAGCGATctg                                               <  2:33999/251‑3 (MQ=255)                                                                                                                                                                                                tGTAAGTTGTGGGATTCAGCCGATTTATTATCAATTTAATCCTCCGTAATGGAGGATTTTATCGTTTCTTTTCACCTTTCCTCCTGTTTATTCTTATTACCCCGTGTTTATGTCTCTGGCTGCCAATTGCTTAAGCAAGATCgg                                                                                                                                                   <  1:142554/144‑1 (MQ=255)                                                                                                                                                                                                tGTAAGTTGTGGGATTCAGCCGATTTATTATCAATTTAATCCTCCGTAATGGAGGATTTTATCGTTTCTTTTCACCTTTCCTCCTGTTTATTCTTATTACCCCGTGTTTATGTCTCTGGCTGCCAATTGCTTAAGCAAGATCgg                                                                                                                                                   >  2:142554/1‑144 (MQ=255)                                                                                                                                                                                                    aGTTGTGGGATTCAGCCGATTTATTATCAATTTAATCCTCCGTAATGGAGGGTTTTATCGTTTCTTTTCACCTTTCCTCCTGTTTATTCTTATTACCCCGTGTTTATGTCTCTGGCTGCCAATTGCTTAAGCAAGATCGGACGGTTACTGTGTTTTACACATTTTTTCCGTCAAACAGTg                                                                                                           >  1:53606/1‑180 (MQ=255)                                                                                                                                                                                                                    cGATTTATTATCAATTTAATCCTCCGTAATGGAGGATTTTATCGTTTCTTTTCACCTTTCCTCCTGTTTATTCTTATTACCCCGTGTTTATGTCTCTGGCTGCCAATTGCTTAAGCAAGATCGGACGGTTAATGTGTTTTACACATTTTTTCCGTCAAACAGTGAGGCAGGCCATGTAGATGTTGTCTGGAGCCGAGATGGTCGTCCGATCGCTTATCGATCAGGGCGTTAAACAAGTATTCGGTTATccc                    <  1:193681/251‑1 (MQ=255)                                                                                                                                                                                                                    cGATTTATTATCAATTTAATCCTCCGTAATGGAGGATTTTATCGTTTCTTTTCACCTTTCCTCCTGTTTATTCTTATTACCCCGTGTTTATGTCTCTGGCTGCCAATTGCTTAAGCAAGATCGGACGGTTAATGTGTTTTACACATTTTTTCCGTCAAACAGTGAGGCAGGCCATGGAGATGTTGTCTGGAGCCGAGATGGTCGTCCGATCGCTTATCGATCAGGGCGTTAAACAAGTATTCGGTTATccc                    <  1:147968/251‑1 (MQ=255)                                                                                                                                                                                                                     gATTTATTATCAATTTAATCCTCCGTAATGGAGGATTTTATCGTTTCTTTTCACCTTTCCTCCTGTTTATTCTTATTACCCCGTGTTTATGTCTCTGGCTGCCAATTGCTTAAGCAAGATCGGACGGTTATTGTGTTTTACACATTTTTTCCGTCAAACCGTGAGGCAGGCCATGGCGATGTTGTCTGTAGCAGAAATGGTGGTCCGATCGCTTATAGATAAGGGGGTTAAACACATATTCGGTTAGCCCg                   >  2:249915/1‑251 (MQ=255)                                                                                                                                                                                                                                aaTTTAATCCTCCGTAATGGAGGATTTTATCGTTTCTTTTCACCTTTCCTCCTGTTTATTCTTATTACCCCGTGTTTATGTCTCTGGCTGCCAATTGCTTAAGCAAGATCGGACGGTTAATGTGTTTTACACATTTTTTCCGTCAAACAGTGAGGCAGGCCATGGAGATGTTGTCTGGAGCCGAGATGGTCGTCCGAGCGCTTATCGATCAGGGCGTTAAACAAGTATTCGGTTATCCCGGAGGCGCAGTc        <  1:423006/251‑1 (MQ=255)                                                                                                                                                                                                                                    tAATCCTCCGTAATGGAGGATTTTATCGTTTCTTGTCCGCTTGCCTTCTGTTTATTCTTATTACCCCGTGGTTATGTCTTTGGCTGCCAAATGCTTAAGCAATATCGGACGGTGAATGTGTTTTACACATGTTTTCCGTAAAGCAGTGAGGCAGGCCATGGAGATGTTGCCGGGAGCCGAGATGGTCGTCCGATCGCTTATCGTTCAGGGCGTTAAACAAGTATTCGGTTAGCTCTGAGGCGCAGTCCtt     <  2:48130/250‑1 (MQ=255)                                                                                                                                                                                                                                      aTCCTCCGTAATGGAGGATTTTATCGTTTCTTTTCACCTTTCCTCCTGTTTATGCTTATTACCCCGTGTTTATGTCTCTGGCTGCCAATTGCTTAAGCAAGATCGGACGGTTAATGTGTTTTACACATTTTTTCCGTCAAACAGTGAGGCAGGCCATGGAGATGTTGTCTGGAGCCGAGATGGTCGTCCGATCTCTTAGCGATCAGGGCGTTAAACAAGTATTCGGTTATCCCGGATGCGCAGTCCTTGat  <  1:378647/251‑1 (MQ=255)                                                                                                                                                                                                                                       tcctccGTAATGGAGGATTTTATCGTTTCTTTTCACCTTTCCTCCTGTTTATTCTTATTACCCCGTGTTTATGTCTCTGGCTGCCAATTGCTTAAGCAAGATCGGACGGTTAATGTGTTTTACACATTTTTTCCGTCAAACAGTGAGGCAGGCCATGGAGATGTTGTCTGGAGCCGAGATGGTCGTCCGATCGCTTATCGATCAGGGCGTTAAAAAAGTATTCGGTTATCCCGGAGGCGCAGTCCTTGat  >  1:346294/1‑250 (MQ=255)                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                     AGCTGCCGGGCGCGATAAAGGCCATCAGTGGATGGAAGAGCAATTAGTCTCAATTTGCAAACGCTAACTGATTGCAGAATAGGTCAGACATGAATGTCTGGTTTATTCTGCATTTTTTATTGAATGTAGAATTTTATTCTGAATGTGTGGGCTCTCTATTTTAGGATTAATTAAAAAAATAGAGAAATTGCTGTAAGTTGTGGGATTCAGCCGATTTATTATCAATTTAATCCTCTGTAATGGAGGATTTTATCGTTTCTTTTCACCTTTCCTCCTGTTTATTCTTATTACCCCGTGTTTATGTCTCTGGCTGCCAATTGCTTAAGCAAGATCGGACGGTTAATGTGTTTTACACATTTTTTCCGTCAAACAGTGAGGCAGGCCATGGAGATGTTGTCTGGAGCCGAGATGGTCGTCCGATCGCTTATCGATCAGGGCGTTAAACAAGTATTCGGTTATCCCGGAGGCGCAGTCCTTGAT  >  NC_000913/85246‑85725

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑