Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	65,454	A→G	R109R (CGT→CGC)	polB ←	DNA polymerase II

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	65,454	0	A	G	100.0%	110.7 / NA	36	R109R (CGT→CGC)	polB	DNA polymerase II
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (11/25);  total (11/25)

GAGGAGGCGTCGCCATTCTCCGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGTTTCAATATCTATAGAAACCCACTTGAGCGGCGGACGATAGTCGGGATGCGGTTTCAGACGGGCATTAACGATAGTGCCATTGTGCATATCACCCTCGACCCACACCGGTGAGGTGATAAACCGCTCCATCAGATAGCGTTCTGGCGGACGCACATCGGCCTCGTAGACGGTAACGCCACCTTCACGCAGGCGCTTTTCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATACA‑CCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAAAGCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGGAATAAACGCCACGGACTCTTGCGGTGCAAGCGTAAC  >  NC_000913/65212‑65681                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                     gcggaggCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGTTTCAATATCAATAGAAACCCACTTGAGCGGCGGACGATAGTCGGGATGCGGTTTCAGACGGGCATTAACGATAGCGCCATTGTGCATATCACCCTCGACCCACACCGGTGAGGTGATAAACCGCTCCATCAGATAGCGTTCTGGCGGGCGCACATc                                                                                                                                                                                                                              <  1:92475/249‑1 (MQ=255)     aggCGTCGCCATTCTCTGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGTTTCAATATCAATAGAAACCCACTTGAGCGGCGGACGATAGTCGGGATGCGGTTTCAGACGGGCATTAACGATAGCGCCATTGTGCATATCACCCTCGACCCACACCGGTGAGGTGATAAACCGCTCCATCAGATAGCGTTCTGGCGGGCGCACATCGGcc                                                                                                                                                                                                                          <  1:366417/251‑1 (MQ=255)           cgccttacgttggCCCCACAATATAAACGATGCGCTGCCCGCAGCCTTACAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGTTTCAATATCAATAGAAACCCACTTGAGCGGCGGACGATAGTCGGGATGCGGTTTCAGACGGGCATTAACGATAGCGCCATTGTGCATATCACCCTCGACCCACACCGGTGAGGTGATAAACCGCTCCATCAGATAGCGTTCTGGCGGGCGCTCATCGGCCTCGTAg                                                                                                                                                                                                                    <  2:330722/240‑1 (MQ=255)                                 ataATCGATGCGCTGCCCGCAGCCGTCCAGGCCGAGGCAGTACAGCTCCCCGTGGCGGGTGGTTTCAATATCAATAGAAACCCACTTGAGCGGCGGACGATAGTCGGGATGCGGTTTCAGACGGGCATTAACGATAGCGCCATTGTGCATATCACCCTCGACCCACACCGGTGAGGTGATAAACCGCTCCATCAGATAGCGTTCTGGCGGGCGca                                                                                                                                                                                                                                  <  2:117940/215‑1 (MQ=255)                                           cgcTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGTTTCAATATCAATAGAAACCCACTTGAGCGGCGGACGATAGTCGGGATGCGGTTTCAGACGGGCATTAACGATAGCGCCATTGTGCATATCACCCTCGACCCACACCGGTGAGGTGATAAACCGCTCCATCAGATAGCGTTCTGGCGGGCGCACATCGGCCTCGTAGACGGTAACGCCACCTTCACGCAGGCGCTTTTc                                                                                                                                                                                    <  2:345117/251‑1 (MQ=255)                                              tGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGTTTCAATATCAATAGAAACCCACTTGAGCGGGGGACGATAGTCGGGCTGCGGTTTTAGGCGGGCATTAACGGTAGGGCCATTTTGCATATCACCCTCGACCCCCACCGGGGAGGTGATAAACCGATCCCTCAGATAGCGTTCTTGCGAGGGCACATCGGGCTCGCTGACGGGAAAGCCACCCTCAcaccggccgtttttgtt                                                                                                                                                                                 >  1:93031/1‑235 (MQ=255)                                                          tCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGTTTCAATATCAATAGAAACCCACTTGAGCGGCGGACGATAGTCGGGATGCGGTTTCAGACGGGCATTAACGATAGCGCCATTGTGCATATCACCCTCGACCCACACCGGTGAGGTGATAAACCGCTCCATCAGATAGCGTTCTGGCGGGCGCACATCGGCCTCGTAGACGGTAACGCCACCTTCACGCAGGCGCTTTTCGTAATTCATCAAt                                                                                                                                                                       <  1:375269/249‑1 (MQ=255)                                                            cAGGCCGATGCAGTACAGCTCACCGTGGCGGGAGGTTTTAATATCCATAGAAACCCAATTGAGCGGCGGACGATACTGGGGATGAGGATTCACACAGGCATTTACGATAGCGCAATTGTGCATATCAACCTCGACCCACCCCGGGGAGGGGATAAAACGCCCCATCAGAAAACGTTCTGGCGGGCGCACATCGGCCCCGAAAACCGGAAAGCCCCACTCACGCCAGCGCTTTTCGTAATTTCTCAAtttgg                                                                                                                                                                   >  1:30244/1‑248 (MQ=255)                                                                             gCTCACCGTGGCGGGTGGTTTCAATATCAATAGAAACCCACTTGAGCGGCGGACGATAGTCGGGATGCGGTTTCAGACGGGCATTAACGATAGCGCCATTGTGCATATCACCCTCGACCCACACCGGTGAGGTGATAAACCGCTCCATCAGATAGCGTTCTGGCGGGCGCACATCGGCCTCGTAGACGGTAACGCCACCTTCACGCAGGCGCTTTTCGTAATTCATCAATTGGCGATGGGCGCGACAGTaa                                                                                                                                                  <  1:146973/251‑1 (MQ=255)                                                                                     tGGCGGGTGGTTTCAATCTCAATAGAACCCCACTTGAACGGCGGACGATAGTCGGGATGCGGTTTCAGACGGGCATTAACGATAGCGCCATTGTGCATATCACCCTCGACCCACACCGGTGAGGTGATAAACCGCTCCATCAGATAGCGTTCTGGCGGGCGCACATCCGCCTCGGAGACGGGAAAGCCACCTTCAAGCAGGCGGTTTTCGTAATTCCTCCATTGGCGACGGGCGCGGCAGTAAAAGccca                                                                                                                                           >  1:397458/1‑248 (MQ=255)                                                                                         gggTGGTTTCAATATCAATAGAAACCCACTTGAGCGGCGGACGATAGTCGGGATGCGGTTTCAGACGGGCATTAACGATAGCGCCATTGTGCATATCACCCTCGACCCACACCGGTGAGGTGATAAACCGCTCCATCAGATAGCGTTCTGGCGGGCGCACATCGGCCTCGTAGACGGTAACGCCACCTTCACGCAGGCGCTTTTCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATACA‑cc                                                                                                                                     <  1:361300/251‑1 (MQ=255)                                                                                                 tcaatatcaataGAAACCCACTTGAGCGGCGGACGATAGTCGGGATGCGGTTTCAGACGGGCATTAACGATAGCGCCATTGTGCATATCACCCTCGACCCACACCGGTGAGGTGATAAACCGCTCCATCAGATAGCGTTCTGGCGGGCGCACATCGGCCTCGTAGACGGTAAAGCCACCTTCACGCAGGCGCTTTTCGTAATTCATCAATTGGCGATGGGCGCGACCGTAAAAGCCATACA‑CCGGCTGGCg                                                                                                                             >  1:221559/1‑251 (MQ=255)                                                                                                      gtcaataGAAGCCCTCTGGAGCGGCGGACGATAGTCGGGATGAGGTTGCAGCCGGGCATTAGCGATAGCGCAATTGTGCCGAGCACCCTCGACCCCCACCGGTGAGGTGATAAACCGCTCCATCAGATAGCGTTTTGGCGGGCGCGCATCGGCCTCGTAGGCGGTAGCGCCACCTTCACGCAGGCGGTTTTCGTAATTCAGCAATTGGCGATGGGCGCGACTGTAAAGGCCATACA‑CCGGCTGGCGGTGa                                                                                                                         <  2:27931/246‑1 (MQ=255)                                                                                                         aataGAAACCCTCTGCAGCTGCGGCCGATAGTCGGGATGCGGTTTCAGACGCTCACTAACGATAGCGCCATTGTGCATATCCCCCTCGACCCTCACCGGTGAGGTGATAAACCGCTCCATCAGATAGCGTTCTGGCGGGCGCACATCGGCCTCGTAGACGGTACCGCCACCTTCACGCAGGCGCTTTTCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATACA‑CCGCCTGGCGGTGAAAAt                                                                                                                     <  2:57941/250‑1 (MQ=255)                                                                                                            aGAAACCCACTTGAGCGGCGGACGATAGTCGGGATGCGGTTTCAGACGGGCATTAACGATAGCGCCATTGTGCATATCACCCTCGACCCACACCGGTGAGGTGATAAACCGCTCCATCAGATAGCGTTCTGGCGGGCGCACATCGGCCTCGTAGACGGTAACGCCACCTTCACGCAGGCGCTTTTCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATACA‑CCGGCTGGCGGTGAAAATCCt                                                                                                                  >  1:45993/1‑251 (MQ=255)                                                                                                             gAAACCCACTTGAGCGGCGGACGATAGTCGGGATGCGGTTTCAGACGGGCATTAACGATAGCGCCATTGTGCATATCACCCTCGACCCACACCGGTGAGGTGATAAACCGCTCCATCAGATAGCGTTCTGGCGGGCGCACATCGGCCTCGTAGACGGTAACGCCACCTTCACGCAGGCGCTTTTCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATACA‑CCGGCTGGCGGTGGAAATCCtt                                                                                                                 >  1:285959/1‑251 (MQ=255)                                                                                                                     cTTGAGCGGCGGACGATAGTCGGGATGCGGTTTCAGACGGGCATTAACGATAGCGCCATTGTGCATATCACCCTCGACCCACACCGGTGAGGTGATAAACCGCTCCATCAGATAGCGTTCTGGCGGGCGCACATCgg                                                                                                                                                                                                                            <  1:77632/137‑1 (MQ=255)                                                                                                                     cTTGAGCGGCGGACGATAGTCGGGATGCGGTTTCAGACGGGCATTAACGATAGCGCCATTGTGCATATCACCCTCGACCCACACCGGTGAGGTGATAAACCGCTCCATCAGATAGCGTTCTGGCGGGCGCACATCgg                                                                                                                                                                                                                            >  2:77632/1‑137 (MQ=255)                                                                                                                       tGAGCGGCGGACGATAGTCGGGATGCGGTTTCAGACGGGCATTAACGATAGCGCCATTGTGCATATCACCCTCGACCCACACCGGTGAGGTGATAAACCGCTCCATCAGATAGCGTTCTGGCGGGCGCACATCGGCCTCGTAGACGGTAACGCCACCTTCACGCAGGCGCTTTTCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATACA‑CCGGCTGGCGGTGAAAATCCTTTAACGCCAg                                                                                                        <  1:423131/250‑1 (MQ=255)                                                                                                                        gAGCGGCGGACGATAGTCGGGATGCGGTTTCAGACGGGCATTAACGATAGCGCCATTGTGCATATCTCCCTCGACCCACACCGGTGAGGTGATAAACCGCTCCATCAGATAGCGTTCTGGCGGGCGCACATCGGCCTCGTAGACGGTAACGCCACCTTCACGCAGGCGCTTTTCGTAATTCATCAATTGGCGATGGTCGCGACAGTAAAGGCCATACA‑CCGGCTGGCGGTGAAAATCCTTTAACGCCAGc                                                                                                       <  1:208596/250‑1 (MQ=255)                                                                                                                               ggACGATAGTCGGGATGCGGTTTCAGACGGGCATTAACGATAGCGCCATTGTGCATATCACCCTCGACCCACACCGGTGAGGTGATAAACCGCTCCATCAGATAGCGTTCTGGCGGGCGCCCATCGGCCTCGTAGACGGTCACGCCACCTTCACGCAGGCGCTTTTCGTAATTCATCAATTGGCGATGGGCGGGGCAGTAAAGGCCATACA‑CCGGCTGGCGGGGAAAATCCTTTaaacaccacggtgtt                                                                                                 >  2:5153/1‑236 (MQ=255)                                                                                                                                    aTAGTCGGGATGCGGTTTCAGACGGGCATTAACGATAGCGCCATTGTGCATATCACCCTCGACCCACACCGGTGAGGTGATAAACCGCTCCATCAGATAGCGTTCTGGCGGGCGAACATAGGCCTCGTAGACGGTAACGCCACCTTCCCGACGGCGCTTTTCGTAATTACTCAATTAGCGATGGGCGCCACAGGAAAGGCAAAACAACCGCCGGGCGGGAAAAACCCTTTAAC‑CCACCGGGGTCAGGCgg                                                                                           >  2:460820/1‑249 (MQ=255)                                                                                                                                        ccggtaTGCGGTTTCAGAAGGGCATTAACGATAGCGCCTTTGGGCATATCACCCTCGACCCCCCCCGGTGAGGTGATAATCCGCTCCATCATATAGCGTTCTGGCGGGCGCACATCGGCCTCGTAGACGGTAACGCCACCGTCACGCAGGCGCGTTTCGTAATTCATCAATTGGCGATGGTCGCGACAGTAAAGGCCATACA‑CCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAACGcc                                                                                      <  2:359698/246‑1 (MQ=255)                                                                                                                                           gtagtcGGTTTCAGACGGGTATTAACGATAGCGCAATTGTGCAGATCCCCCCCGACCCACACCGGTGAGGTGATAAACTGCTCCATCAGATAGCGTTCTGGCGGGCGCACATCGGCCTCGTAGACGGTAACGCCACCTTCACGCAGGCGCTTTTCGTAATTCATCAAGTGGCGATGGGCGCGACAGTAAAGGCCATACA‑CCGGCTGGCGGTGAAAATCCTTTAACGCCACCGGTGTCAGGCGAACGCCTTg                                                                                   <  2:4662/246‑1 (MQ=255)                                                                                                                                                    ttCAGACGGGCATTAACGATAGCGCCATTGTTCATACCACCCGCGACCCACCCCGGTGAGGTGAGAAACCGCTCCATCAGATAGCGTTCTGGCGGGCGCACATCGGCCTCGTAGACGGTAACGCCACCTTCACGCCGGCGCTGTTCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATACA‑CCGGCGGGCGGTGAAAATCCGTTAACGCCAGCGGTGTCAGGCGAACGCCTTGTTCACCCt                                                                           <  2:67694/250‑1 (MQ=255)                                                                                                                                                    ttCAGACGGGCATTAACGATAGCGCCATTGTGCATATCACCCTCGACCCACACCGGTGAGGTGATAAACCGCTCCATCAGATAGCGTTCTGGCGGGCGCACATCGGCCTCGTAGACGGTAACGCCACCTTCACGCAGGCGCTTTTCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATACA‑CCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAACGCCTTGTTCACCCTg                                                                          >  1:352382/1‑251 (MQ=255)                                                                                                                                                            ggCATTAATGATAGCGCCATTGTGCATATCACCCTCGACCCACACCGGTGAGGTGATAAACCGCTCCATCAGATAGCGTTCTGGCGGGCGCACATCGGCCTCGTAGACGGTAACGCCACCTTCACGCAGGCGCTTTTCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATACA‑CCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAACGCCTTGt                                                                                  <  1:149253/235‑1 (MQ=255)                                                                                                                                                                    cGATAGCGCCATTGTGCATATCACCCTCGACCCACACCGGTGAGGTGATAAACCGCTCCATCAGATAGCGTTCTGGCGGGCGCACATCGGCCTCGTAGACGGTAACGCCACCTTCACGCAGGCGCTTTTCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATACA‑CCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAACGCCTTGTTCACCCTGCAAAATATGCTGAgcg                                                          <  2:45993/251‑1 (MQ=255)                                                                                                                                                                     gATAGCGCCCTTGTGCTTATCACCCGCGACCCACACCGGTGAGGTGATAAACCGCTCCATCAGATAGCGTTCTGGCGGGCGCACATCGGCCTCGTAGACGGTAACGCCACCTTCACGCAGGCGCTTTTCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATACA‑CCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAACGCCTTGTTCACCCTGCAAAATATGCTGAgcgc                                                         <  2:167010/251‑1 (MQ=255)                                                                                                                                                                                   gCATATCACCCTCGACCCACACCGGTGAGGTGATAAACCGCTCCATCAGATAGCGTTCTGGCGGGCGCACATCGGCCTCGTAGACGGTAACGCCACCTTCACGCAGGCGCTTTTCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATACA‑CCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAACGCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAACCTGATc                                            <  1:311905/250‑1 (MQ=255)                                                                                                                                                                                      tatCACCCTCGACCCACACCGGTGAGGTGATAAACCGCTCCATCAGATAGCGTTCTGGCGGGCGCACATCGGCCTCGTAGACGGTAACGCCACCTTCACGCAGGCGCTTTTCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATACA‑CCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTTCGGCGAACGCCCTTTTCACCCTGCAAAATATGCTGAGCGCGGGGAACCTGATcggcg                                        >  1:72002/1‑251 (MQ=255)                                                                                                                                                                                           cccTCGCCCCACACCGGTGAGGTGCTAAACCGCTCCATCAGTTATCGTACTGGCGGGCGCACATCGGCCTCGGAGACGGTAACGCCACCTTCACTCAGGCGCTTATCGTAATTCAGCAATTGGCGATGGGCGCGACAGTAAAGGCCATACA‑CCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAACGCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAACCTGATCTGCGGGAAt                                   <  2:86203/251‑1 (MQ=255)                                                                                                                                                                                                         cGGTGAGGTGATAAACCGCTCCATCAGATAGCGTTCTGGCGGGCGCACATCGGCCTCGTAGACGGTAACGCCACCTTCACGCAGGCGCTTTTCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATACA‑CCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAACGCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGGAATAAACGCCACGGAct                     <  1:40164/251‑1 (MQ=255)                                                                                                                                                                                                            ggaggtgaTAAACCTCTCCATCAGATAGCGTTCTGGCGGGCGCACATCGGCCGCGGAGACGGTAACGCCACCTTCACGCAGGCGCTTTTCGTAAGTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATCCA‑CCGGCGGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAACGCCTTGTTCACCCTGCAAAATATGCTGAGCGCGTGGAACCTGATCGGCGGGAATAAACGCCACGGAGTCtt                  <  2:304728/250‑1 (MQ=255)                                                                                                                                                                                                             gaggtgaTAAACCGCTCCATCAGATAGCGTTCTGGCGGGCGCACATCGGCCTCGTAGACGGTAACGCCACCTTCACGCAGGCGCTTTTCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATACA‑CCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAACGCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGGAATAAACGCCACGGACTCTTg                 <  1:237215/251‑1 (MQ=255)                                                                                                                                                                                                                             ccATCAGATAGCGTTCTGGCGGGCGCACATCGGCCTCGTAGACGGTAACGCCACCTTCACGCAGGCGCTTTTCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATACA‑CCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAACGCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGGAATAAACGCCACGGACTCTTGCGGTGCAAGCGTAAc  <  1:284149/250‑1 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                     GAGGAGGCGTCGCCATTCTCCGGCCCCAGCATATAAACGATGCGCTGCCCGCAGCCTTCCAGGCCGATGCAGTACAGCTCACCGTGGCGGGTGGTTTCAATATCTATAGAAACCCACTTGAGCGGCGGACGATAGTCGGGATGCGGTTTCAGACGGGCATTAACGATAGTGCCATTGTGCATATCACCCTCGACCCACACCGGTGAGGTGATAAACCGCTCCATCAGATAGCGTTCTGGCGGACGCACATCGGCCTCGTAGACGGTAACGCCACCTTCACGCAGGCGCTTTTCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATACA‑CCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAAAGCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGGAATAAACGCCACGGACTCTTGCGGTGCAAGCGTAAC  >  NC_000913/65212‑65681

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑