Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 83,371 G→A G53G (GGC→GGT)  leuA ← 2‑isopropylmalate synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00091383,3710GA89.1% 118.0 / 6.0 47G53G (GGC→GGT) leuA2‑isopropylmalate synthase
Reads supporting (aligned to +/- strand):  ref base G (1/4);  new base A (24/17);  total (26/21)
Fisher's exact test for biased strand distribution p-value = 1.63e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.93e-02

AATTACGGGCGCGTTTCACCATATAGATAGCGCGTTCGATCACCTCGTCCAGCGTGCTGCGCAGCTTGGTGGCGATGTGCATTGGCGAAGTGGCAATAAAGGTATGAATACGGAAGGCTTCGGCGACTTTCAGGGATTCGGCCGCCACGTCGATATCTTTTTCCACGCAGCGAGCTAACGCACATACGCGGCTGTTTTTAACCTGGCGGGCGATGGTTTGCACCGATTCAAAATCGCCCGGCGAAGAGACGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGTTTTTCTTTCACACTCAAGCTTGCCTGTAACGCCTGTTCACCGTCGCGCAATGTGGTATCGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTG  >  NC_000913/83136‑83619
                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                        
aaTTACGGGCGCGTTTCACCATATAGATAGCGCGTTCGATCACTTCATCCAGCGTGCTGCGCAGCTTGGTGGCGATGTGCATTGGCGAAGTGGCAATAAAGGTATGAATACGGAAGGCTTCGGCGACTTTCAGGGATTCGGCCGCCACGTCGATATCTTTCTCCACGCAGCGAGCTAACGCGCATACGCGGCTGTTTTTAACCTGGCGAGCGATGGTTTGCACTGATTCAAAATCACCCGGCGAAGAAACg                                                                                                                                                                                                                                           >  1:10354/1‑251 (MQ=255)
   tACGGGCGCGTTTCACCATATAGATAGCGCGTTCGATCACTTCATCCAGCGTGCTGCGCAGCTTGGTGGCGATGTGCATTGGCGAAGTGGCAATAAAGGTATGAATACGGAAGGATTCGGCGACTTTCAGGGATTCGGCCGCCACGTCGATATCTTTCTCCACGCAGCGAGCTAACGCGCATACGCGGCTGTTTTTAACCTGGCGAGCGATGGTTTGCACTGATTCAAAATCACCCGGCGAAGAAACgggg                                                                                                                                                                                                                                        >  1:453250/1‑251 (MQ=255)
     cGGGCGCGTTTCACCATATAGATAGCGCGTTCGATCACTTCATCCAGCGTGCTGCGCAGCTTGGTGGCGATGTGCATTGGCGAAGTGGCAATAAAGGTATGAATACGGAAGGCTTCGGCGACTTTCAGGGATTCGGCCGCCACGTCGATATCTTTCTCCACGCAGCGAGCTAACGCGCATACGCGGCTGTTTTTAACCTGGCGAGCGATGGATTGCACTGATTCAAAATCACCCGGCGAAGAAACGGGGa                                                                                                                                                                                                                                       >  1:43203/1‑250 (MQ=255)
          gcgTTTCACCATATAGATAGCGCGTTCGATCACTTCATCCAGCGTGCTGCGCAGCTTGGTGGCGATGTGCATTGGCGAAGTGGCAATAAAGGTATGAATACAGAAGGCTTCGGCGACTTTCAGGGATTCGGCCGCCACGTCGATATCTTTCTCCACGCAGCGAGCTAACGCGCATACGCGGCTGTTTTTAACCTGGCGCGCGATGGTTTGCACTGATTCAAAATCACCCGGCGAAGAAACGGGGAAACCg                                                                                                                                                                                                                                  >  2:50487/1‑250 (MQ=255)
            gTTTCACCATATAGATAGCGCGTTCGATCACTTCATCCAGCGTGCTGCGCAGCTTGGTGGCGATGTGCATTGGCGAAGTGGCAATAAAGGTATGAATACGGAAGGCTTCGGCGACTTTCAGGGATTCGGCCGCCACGTCGATATCTTTCTCCACGCAGCGAGCTAACGCGCATACGCGGCTGTTTTTAACCTGGCGCGCGCTGGTTTGCACTGATTCAAAATCACCCGGCGAAGAAACGGGGAAACCGACt                                                                                                                                                                                                                               >  2:237427/1‑251 (MQ=255)
            gTTTCACCATATAGATAGCGCGTTCGATCACTTCATCCAGCGTGCTGCGCAGCTTGGTGGCGATGTGCATTGGCGAAGTGGCAATAAAGGTATGAATACGGAAGGCTTCGGCGACTTTCAGGGATTCGGCCGCCACGTCGATATCTTTCTCCACGCAGCGAGCTAACGCGCATACGCGGCTGTTTTTAACCTGGCGAGCGATGGTTTGCACTGATTCAAAATCACCCGGCGAAGAAACGGGGAAACCGACt                                                                                                                                                                                                                               <  2:201533/251‑1 (MQ=255)
                   cATATAGATAGCGCGTTCGATCACCTCGTCCAGCGTGCTGCGCAGCTTGGTGGCGATGTGCATTGGCGAAGTGGCAATAAAGGTATGAATACGGAAGGCTTCGGCGACTTTCAGGGATTCGGCCGCCACGTCGATATCTTTTTCCACGCAGCGAGCTAACGCACATACGCGGCTGTTTTTAACCTGGCGGGCGATGGTTTGCACCGATTCAAAATCGCCCGGCGAAGAGACGGGGAAACCGACTTCCATCa                                                                                                                                                                                                                        <  1:38693/251‑1 (MQ=255)
                                    cgatcccttcatCCAGCGTGCGGCGCAGCTTGGTGGCGATGTGCATTGGCGAAGTGGCAATAAAGGTATGAATACGGAAGGCTTCGGCGACTTTCAGGGATTCGGCCGCCACGTCGATATCTTTCTCCACGCAGCGAGCTAACGCGCATACGCGGCTGTTTTTAACCTGGCGAGCGATGGTTTGCACTGATTCAAAATCACCCGGCGAAGAAACGGGGAAACCGACTTCCATCACGTCAACACCCATACg                                                                                                                                                                                                        <  2:139856/239‑1 (MQ=255)
                                       tcacttcatCCAGCGTGCTGCGCAGCTTGGTGGCGATGTGCATTGGCGAAGTGGCAATAAAGGTATGAATACGGAAGGCTTCGGCGACTTTCAGGGATTCGGCCGCCACGTCGATATCTTTCTCCACGCAGCGAGCTAACGCGCATACGCGGCTGTTTTTAACCTGGCGAGCGATGGTTTGCACTGATTCAAAATCACCCGGCGAAGAAACGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTc                                                                                                                                                                                                     >  1:361407/9‑250 (MQ=255)
                                        cacttcatCCAGCGTGCTGCGCAGCTTGGTGGCGATGTGCATTGGCGAAGTGGCAATAAAGGTATGAATACGGAAGGCTTCGGCGACTTTCAGGGATTCGGCCGCCACGTCGATATCTTTCTCCACGCAGCGAGCTAACGCGCATACGCGGCTGTTTTTAACCTGGCGAGCGATGGTTTGCACTGATTCAAAATCACCCGGCGAAGAAACGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCa                                                                                                                                                                                                    >  1:153038/8‑250 (MQ=255)
                                         acttcatCCAGCGTGCTGCGCAGCTTGGTGGCGATGTGCATTGGCGAAGTGGCAATAAAGGTATGAATACGGAAGGCTTCGGCGACTTTCAGGGATTCGGCCGCCACGTCGATATCTTTCTCCACGCAGCGAGCTAACGCGCATACGCGGCTGTTTTTAACCTGGCGAGCGATGGTTTGCACTGATTCAAAATCACCCGGCGAAGAAACGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCa                                                                                                                                                                                                    >  1:298776/7‑249 (MQ=255)
                                              atCCAGCGTGCTGCGCAGCTTGGTGGCGATGTGCATTGGCGAAGTGGCAATAAAGGTATGAATACGGAAGGCTTCGGCGACTTTCAGGGATTCGGCCGCCACGTCGATATCTTTCTCCACGCAGCGAGCTAACGCGCATACGCGGCTGTTTTTAACCTGGCGAGCGATGGTTTGCACTGATTCAAAATCACCCGGCGAAGAAACGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGcc                                                                                                                                                                                              >  1:376253/2‑250 (MQ=255)
                                                                cTTGGTGGCGATGTGCATTGGCGAAGTGGCAATAAAGGTATGAATACGGAAGGCTTCGGCGACTTTCAGGGATTCGGCCGCCACGTCGCTATCTTTCTCCACGCAGCGAGCTAACGCGCATACGCGGCTGTTTTTAACCTGGCGAGCGATGGTTTGCACTGATTCAAAATCCCCCGGCGAAGAAACGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGttttt                                                                                                                                                                           >  2:215394/1‑251 (MQ=255)
                                                                    gtggCGATGTGCATTGGCGAAGTGGCAATAAAGGTATGAATACGGAAGGCGTCGGCGACTTTCAGGGATTCGGCCGCCACGTCGATATCTTTCTCCACGCAGCGAGCTAACGCGCATACGCGGCTGTTTTTAACCTGGCGAGCGATGGTTTGCACTGATTCAAAATCACCCGGCGAAGAAACGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGTTtttcttt                                                                                                                                                                       <  2:222038/251‑1 (MQ=255)
                                                                         gATGTGCATTGGCGAAGTGGCAATAAAGGTATGAATACGGAAGGCTTCGGCGACTTTCAGGGATTCGGCCGCCACGTCGCTATCTTTATCCACGCAGCGAGCTAACGCGCATACGCGGCTGTTTTTAACCTGGCGAGCGATGGTTTGCACTGATTCAAAATCACCCGGCGAAGAAACGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGTTTTTCTTTCacac                                                                                                                                                                  >  2:304303/1‑251 (MQ=255)
                                                                          aTGTGCATTGGCGAAGTGGCAATAAAGGTATGAATACGGAAGGCTTCGGCGACTTTCAGGGATTCGGCCGCCACGTCGATATCTTTCTCCACGCAGCGAGCTAACGCGCATACGCGGCTGTTTTTAACCTGGCGAGCGATGGTTTGCACTGATTCAAAATCACCCGGCGAAGAAACGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGTTTTTCTTTCACACt                                                                                                                                                                 <  1:40990/251‑1 (MQ=255)
                                                                              gCATTGGCGAAGTGGCAATAAAGGTATGAATACGGAAGGCTTCGGCGACTTTCAGGGATTCGGCCGCCACGTCGATATCTTTTTCCACGCAGCGAGCTAACGCACATACGCGGCTGTTTTTAACCTGGCGGGCGATGGTTTGCACCGATTCAAAATCGCCCGGCGAAGAGACGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGTTTTTCTTTCACACTCaa                                                                                                                                                              <  1:9677/250‑1 (MQ=255)
                                                                                  tGGCGAAGTGGCAATAAAGGTATGAATACGGAAGGCTTCGGCGACTTTCAGGGATTCGGCCGCCACGTCGATATCTTTCTCCACGCAGCGAGCTAACGCGCATACGCGGCTGTTTTTAACCTGGCGAGCGATGGTTTGCACTGATTCAAAATCACCCGGCGAAGAAACGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGTTTTTCTTTCACACTCAAGCtt                                                                                                                                                          <  2:210016/250‑1 (MQ=255)
                                                                                              aaTAAAGGTATGAATACGGAAGGCTTCGGCGACTTTCAGGGATTCGGCCGCCACGTCGATATCTTTCTCCACGCAGCGAGCTAACGCGCATACGCGGCTGTTTTTAACCTGGCGAGCGATGGTTTGCACTGATTCAAAATCACCCGGCGAAGAAACGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGTTTTTCTTTCACACTCAAGCTTGCCTGTAACGCCt                                                                                                                                             >  1:415962/1‑251 (MQ=255)
                                                                                                       gtGAATACGGAAGGCTTCGGCTAATTTCAGGGAGTCGGCCGCCACGTCGATATCTTTCTCCACGCAGCGAGCTAACGCGCATACGCGGCTGTTTTTAACCTGGCGAGCGATGGTTTGCACTGATTCAAAATCACCCGGCGAAGAAACGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGTTTTTCTTTCACACTCAAGCTTGCCTGTAACGCCTGTTCACCg                                                                                                                                     <  2:275203/249‑1 (MQ=255)
                                                                                                         gAATACGGAAGGCTTCGGCGACTTTCAGGGATTCGGCCGCCACGGCGATATCTTTCTCCACGCAGCGAGCTAACGCGCATACGCGGCTGTTTTTAACCTGGCGAGCGATGGTTTGCACTGATTCAAAATCACCCGGCGAAGAAACGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGTTTTTCTTTCACACTCAAGCTTGCCTGTAACGCCTGTTCACCGTcg                                                                                                                                  <  2:376253/251‑1 (MQ=255)
                                                                                                                      ttCGGCGACTTTCAGGGATTCGGCCGCCACGTCGATATCTTTCTCCACGCAGCGAGCTAACGCGCATACGCGGCTGTTTTTAACCTGGCGAGCGATGGTTTGCACTGATTCAAAATCACCCGGCGAAGAAACGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGTTTTTCTTTCACACTCAAGCTTGCCTGTAACGCCTGTTCACCGTCGCGCAATGTGGTAt                                                                                                                     >  2:368047/1‑251 (MQ=255)
                                                                                                                        cGGCGACTTTCAGGGATTCGGCCGCCACGTCGATATCTTTCTCCACGCAGCGAGCTAACGCGCATACGCGGCTGTTTTTAACCTGGCGAGCGATGGTTTGCACTGATTCAAAATCACCCGGCGAAGAAACgg                                                                                                                                                                                                                                          <  1:453110/132‑1 (MQ=255)
                                                                                                                        cGGCGACTTTCAGGGATTCGGCCGCCACGTCGATATCTTTCTCCACGCAGCGAGCTAACGCGCATACGCGGCTGTTTTTAACCTGGCGAGCGATGGTTTGCACTGATTCAAAATCACCCGGCGAAGAAACGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGTTTTTCTTTCACACTCAAGCTTGCCTGTAACGCCTGTTCACCGTCGCGCAATGTGGTATCg                                                                                                                   <  1:37575/251‑1 (MQ=255)
                                                                                                                        cGGCGACTTTCAGGGATTCGGCCGCCACGTCGATATATTTCTCCACGCAGCGAGCTAACGCGCATACGCGGCTGTTTTTAACCTGGCGAGCGATGGTTTGCACTGATTCAAAATCACCCGGCGAAGAAACgg                                                                                                                                                                                                                                          >  2:453110/1‑132 (MQ=255)
                                                                                                                            gACTTTCAGGGATTCGGCCGCCACGTCGATATCTTTCTCCACGCAGCGAGCTAACGCGCATACGCGGCTGTTTTTAACCTGGCGAGCGATGGTTTGCACTGATTCAAAATCACCCGGCGAAGAAACGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGTTtttct                                                                                                                                                                         <  1:326480/193‑1 (MQ=255)
                                                                                                                            aaCTTTCAGGGATTCGGCCGCCACGTCGATATCTTTCTCCACGCAGCGAGCTAACGCGCATACGCGGCTGTTTTTAACCTGGCGAGCGATGGTTTGCACTGATTCAAAATCACCCGGCGAAGAAACGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCCGTTtttct                                                                                                                                                                         >  2:326480/2‑193 (MQ=255)
                                                                                                                                ttCAGGGATTCGGCCGCCACGTCGATATCTTTCTCCACGCAGCGAGCTAACGCGCATACGCGGCTGTTTTTAACCTGGCGAGCGATGGTTTGCACTGATTCAAAATCACCCGGCGAAGAAACGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGTTTTTCTTTCACACTCAAGCTTGCCTGTAACGCCTGTTCACCGTCGCGCAATGTGGTATCGAAaataat                                                                                                           >  2:396098/1‑251 (MQ=255)
                                                                                                                                  cAGGGATCCGGCCGCCTCGTCGATATCTTTCTCCACGCAGCGAGCTAACGCGCATACGCGGCTGTTTTTAACCTGGCGAGCGATGGTTTGCACTGATTCAAAATCACCCGGCGAAGAAACGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGTTTTTCTTTCACACTCAAGCTTGCCTGTAACGCCTGTTCACCGTCGCGCAATGTGGTATCGAAAATAATGa                                                                                                         <  2:88233/251‑1 (MQ=255)
                                                                                                                                     ggATTCGGCCGCCACGTCGATATCTTTCTCCACGCAGCGAGCTAACGCGTATTCGCGGCTGTTTTTAACCTGGCGAGCGATGGTTTGCACTGATTCAAAATCACCCGGCGAAGAAACGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGTGCCAGCGCAATTTGCAGTTTTTCTTTCACACTCAAGCTTGCCTGTATCGCCTGTTCATCGTCGCGCAATGTGGTATCGAAAATAATGACtt                                                                                                      <  1:395422/251‑1 (MQ=255)
                                                                                                                                               gccACGTCGATATCTTTCTCCACGCAGCGAGCTAACGCGCATACGCGGCTGTTTTTAACCTGGCGAGCGATGGTTTGCACTGATTCAAAATCACCCGGCGAAGAAACGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGTTTTTCTTTCACACTCAAGCTTGCCTGTAACGCCTGTTCACCGTCGCGCAATGTGGTATCGAAAATAATGACTTGCTGGCTCAt                                                                                            <  1:445738/251‑1 (MQ=255)
                                                                                                                                                   cGTCGATATCTTTCTCCACGCAGCGAGCTAACGCGCATACGCGGCTGTTTTTAACCTGGCGAGCGATGGTTTGCACTGATTCAAAATCACCCGGCGAAGAAACGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGTTTTTCTTTCACACTCAAGCTTGCCTGTAACGCCTGTTCACCGTCGCGCAATGTGGTATCGAAAATAATGACTTGCTGGCTCATGGtt                                                                                        <  1:368047/251‑1 (MQ=255)
                                                                                                                                                         tatCTTTCTCCACGAAGCGAGCTAACGCGCATACGCGGCTGTTTTTAACCTGGCGAGCGATGGTTTGCACTGATTCAAAATCACCCGGCGAAGAAACGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGTTTTTCTTTCACACTCAAGCTTGCCTGTAACGCCTGTTCACCGTCGCGCAATGTGGTATCGAAAATAATGACTTGCTGGATCATGGTTTGGGTc                                                                                  >  1:378624/1‑251 (MQ=255)
                                                                                                                                                             tttctCCACGCAGCGAGCTAACGCGCATACGCGGCTGTTTTTAACCTGGCGAGCGATGGTTTGCACTGATTCAAAATCACCCGGCGAAGAAACGGGGAAACCGACTTCCATCACGTCAACACCCACACGCTCAAGGGCCAGCGCAATTTGCAGTTTTTCTTTCACACTCCAGCTTGCCTGGAACGCCTGTTCACCGTCGCGCCATGTTGTATCGACAATAATGTCATGCTGGCTCATGGTTTGGGTCCTTg                                                                              >  2:381252/5‑251 (MQ=255)
                                                                                                                                                                  ccACGCAGCGCGCTAACGCGCATACGCGGCTGTTTTTAACCTGGCGAGCGATGGTTTGCACTGATTCAAAATCACCAGGAGAAGAAACGGGGAAACCGACTTCCATCACGTCAACACCCATACGATCAAGGGCCAGCGCAATTTGCAGTTTTTCTTTCACACTCAAGCTTGCCTGTAACGCCTGTTCACCGTCACGCAATGTGGTATAGAAAATAATGTCTTGCTGGCGCATGGTTTGGGTCCTTGtctgt                                                                         >  1:466671/1‑249 (MQ=255)
                                                                                                                                                                    aCGCAGCGAGCTAACGCGCATACGCGGCTGTTTTTAACCTGGCGAGCGATGGTTTGCACTGATTCAAAATCACCCGGCGAAGAAACGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGTTTTTCTTTCACACTCAAGCTTGCCTGTAACGCCTGTTCACCGTCGCGCAATGTGGTATCGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCttt                                                                       <  1:237427/251‑1 (MQ=255)
                                                                                                                                                                                      caTACGCGGCTGTTTTTAACCTGGCGAGCGATGGTTTGCACTGATTCAAAATCACCCGGCGAAGAAACGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGTTTTTCTTTCACACTCAAGCTTGCCTGTAACGCCTGTTCACCGTCGCGCAATGTGGTATCGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCgg                                                     >  1:193128/1‑251 (MQ=255)
                                                                                                                                                                                             ggCTGTTTTTAACCTGGCGAGCGATGGTTTGCACTGATTCAACATCACCCGGCGAAGAAACGGGGAAACAGACTTCCATCACGTCAACACCCATACGCTCAAGGGCAAGCGCAATTTGCAGTTTTTCTTTCACACTCAAGCTTACCTGTAACGCCTGTTCACCGTCGCGCAATGTGGTATCGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATaa                                               >  1:435853/1‑250 (MQ=255)
                                                                                                                                                                                                    tttAACCTGGCGAGCGATGGTTTGCACTGATTCAAAATCACCCGGCGAAGAAACGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGTTTTTCTTTCACACTCAAGCTTGCCTGTAACGCCTGTTCACCGTCGCGCAATGTGGTATCGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAAcc                                        <  1:372571/250‑1 (MQ=255)
                                                                                                                                                                                                     ttaaccatgcgagcgATGGGTTGCACTGATTCAAAATCACCCGGCGAAGAAACGGTGAAACCGAATGCCATCACGTCAACACCCCTACGCTCAAGGGCCAGCGCAATTTGCAGTTTTGCTTTCACACTCAAGCTTGCCTGTAACGCCTGTTCACCGGCGCGCAATGTGGTATCGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAAccc                                       <  2:130258/238‑1 (MQ=255)
                                                                                                                                                                                                         ccTGGCGAGCGATGGTTTGCACTGATTCCAAATCACCCGGCGAAGAAACGGGGACACCGCCTTCCATCACGTAAACACCCATACGCTCAAGGACCAGCGCAATTTGCAGTTTTTCTTTCCCACTCAAGCTTGCCTGTAACGCCTGTTCACCGTCGCGCAATGTGGTATCGTAAATATTGACTTGTTGGCTCATGGTTTGGGTCCTTGACTCTTTTAGAGCGACTAGATTCGGGCATaaaaaaatcagcgc                                   >  1:69104/1‑243 (MQ=255)
                                                                                                                                                                                                           tGGCGAGCGATGGTTTGCACTGATTCAAAATCACCCGGCGAAGAAACGGGGAAACCGACTTCAATCACGTCAACACCCATACGCTCAAGGGCCAGCGAAATTTGCAGTTTTTCTTTCACACTCAAGCTTGCCTGTAACGCCTGTTCACCGTCGCGCACTGTGGTATCAACACTAATAACTTGCTGGATCATGGTTTGGGTCCATGTCTCTTTTAACGCGCCTCGCTTCGGGCATAAAAAACACCGCGCAAt                                >  2:347416/1‑251 (MQ=255)
                                                                                                                                                                                                                            cactgATTCAAAATCACCCGGCGAAGAAACGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGACCAGCGCAATTTGCAGTTTTTCTTTCACACTCAAGCTTGCCTGTAACGCCTGTTCACCGTCGCGCAATGTGGAATCGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGCGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTtttgtt               >  1:11077/5‑251 (MQ=255)
                                                                                                                                                                                                                             aCCGATTCAAAATCGCCCGGCGAAGAGACGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGTTTTTCTTTCACACTCAAGCTTGCCTGTAACGCCTGTTCACCGTCGCGCAATGTGGTATCGAAAATAATGACTTGCTGGCt                                                                                               <  1:91266/170‑1 (MQ=255)
                                                                                                                                                                                                                             aCCGATTCAAAATCGCCCGGCGAAGAGACGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGTTTTTCTTTCACACTCAAGCTTGCCTGTAACGCCTGTTCACCGTCGCGCAATGTGGTATCGAAAATAATGACTTGCTGGCt                                                                                               >  2:91266/1‑170 (MQ=255)
                                                                                                                                                                                                                                  ttCAAAATCACCCGGCGAAGAAACGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGTTTTTCTTTCACACTCAAGCTTGCCTGTAACGCCTGTTCACCGTCGCGCAATGTGGTATCGAAAATAATTACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTg         >  1:18266/1‑251 (MQ=255)
                                                                                                                                                                                                                                        aTCGCCCGGCGAAGAGACGGGGAAACCTACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGTTTTTCTTTCACACTCAAGCTTGCCTGTAACGCCTGTTCACCGTCGCGCAATGTGGTATCGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGACTCTTT‑AGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTg  <  2:316075/251‑1 (MQ=255)
                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                        
AATTACGGGCGCGTTTCACCATATAGATAGCGCGTTCGATCACCTCGTCCAGCGTGCTGCGCAGCTTGGTGGCGATGTGCATTGGCGAAGTGGCAATAAAGGTATGAATACGGAAGGCTTCGGCGACTTTCAGGGATTCGGCCGCCACGTCGATATCTTTTTCCACGCAGCGAGCTAACGCACATACGCGGCTGTTTTTAACCTGGCGGGCGATGGTTTGCACCGATTCAAAATCGCCCGGCGAAGAGACGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGTTTTTCTTTCACACTCAAGCTTGCCTGTAACGCCTGTTCACCGTCGCGCAATGTGGTATCGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTG  >  NC_000913/83136‑83619

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑