Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,696,909 G→A G151E (GGA→GAA)  bcsE → c‑di‑GMP‑binding protein BcsE

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,696,9090GA96.9% 94.6 / ‑3.9 32G151E (GGA→GAA) bcsEc‑di‑GMP‑binding protein BcsE
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base A (16/15);  total (16/16)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.83e-01

GCCGGAAAAAATAAAATTATTTTCAATGCTAAATCATGAAAAAGGTCTATACTATTTGACCCGTGATTTGCAGTGTTCTATTGATCCCCATAATTACCTTTTTATTCTTGTTTGCGCAAATAACGCAT‑GGCAAAACATTCCTGCCGAGCGGCTTCGCTCATGGTTGGATAAAATGAATAAATGGAGCAGGTTAAACCATTGTTCGCTTTTGGTAATTAATCCCGGAAATAATAACGATAAACAATTTTCATTGTTGCTTGAGGAATACCGTTCACTTTTTGGTCTTGCCAGTTTGCGTTTTCAGGGTGACCAACATTTGCTGGATATTGCCTTCTGGTGCAACGAAAAAGGGGTCAGCGCCCGTCAGCAGCTTAGCGTTCAGCAACAAAATGGTATCTGGACATTAGTTCAAAGCGAAGAGGCGGAGATCCAACCACGCAGCGACGAAAAACG  >  NC_000913/3696685‑3697137
                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                    
gCCGGAAAAAATAAAATTATTTTCAATGCTAAATCATGAAAAAGGTCTATACTATTTGACCCGTGATTTGCAGTGTTCTATTGATCCCCATAATTACCTTTTTATTCTTGTTTGCGCAAATAACGCAT‑GGCAAAACATTCCTGCCGAGCGGCTTCGCTCATGGTTGGATAAAATGAATAAATGGAGCAGGTTAAACCATTGTTCGCTTTTGGTAATTAATCCCGAAAATAATAACGATAAACAATTTTCAt                                                                                                                                                                                                            <  1:410583/251‑1 (MQ=255)
 acGGAAAAAATAAAATTATTTTCAATGCTAAATCATGAAAACGGTCTATACTATTTGACCCGTGATTTGCAGTGTTCTATTGATCCCCATAATTACCTTTTTATTCTTGTTTGCGCAAATAACGCAT‑GGCAAAACATTCCTGCCGAGCGGCTTCGCTCATGGTTGGATAAAATGAATAAATGGAGCAGGTTAAACCATTGTTCGCTTTTGGTAATTAATCCCGAAAATAATAACGATAAACAATTTTAAtt                                                                                                                                                                                                           >  2:348379/2‑251 (MQ=255)
    gAAAAAATAAAAGTATTTTCAATGCTAAATCATGAAAAAGGTCTATACTATTTGACCCGTGATTTGCAGTGTTCTATTGATCCCCATAAATACCTTTTTATTCTTGTTTGCGCAAATAACGCAT‑GGCAAAACATTCCTGCCGAGCGGCTTCGCTCATGGTTGGATAAAATGAATAAATGGAGCAGGTTAAACCATTGTTCGCTTTTGGTAATTAATCCCGAAAATAATAACGATAAACAATTTTCAttgt                                                                                                                                                                                                         <  2:135486/250‑1 (MQ=255)
                 tattTTCAATGCTAAATCATGAAAAAGGTCTATACTATTTGACCCGTGATTTGCAGTGTTCTATTGATCCCCATAATTACCTTTTTATTCTTGTTTGCGCAAATAACGCAT‑GGCAAAACATTCCTGCCGAGCGGCTTCGCTCATGGTTGGATAAAATGAATAAATGGAGCAGGTTAAACCATTGTTCGCTTTTGGTAATTAATCCCGAAAATAATAACGATAAACAATTTTCATTGTTGCTTGAGGAATAc                                                                                                                                                                                           >  1:418798/1‑251 (MQ=255)
                       cAATGCTAAATCATGAAAAAGGTCTATACTATTTGACCCGTGATTTGCAGTGTTCTATTGATCCCCATAATTACCTTTTTATTCTTGTTTGCGCAAATAACGCAT‑GGCAAAACATTCCTGCCGAGCGGCTTCGCTCATGGTTGGATAAAATGAATAAATGGAGCAGGTTAAACCATTGTTCGCTTTTGGTAATTAATCCCGAAAATAATAACGATAAACAATTTTCATTGTTGCTTGAGGAATACCGTTc                                                                                                                                                                                      >  1:108810/1‑250 (MQ=255)
                       cAATGCTAAATCATGAAAAAGGTCTATACTATTTGACCCGTGATTTGCAGTGTTCTATTGATCCCCATAATTACCTTTTTATTCTTGTTTGCGCAAATAACGCAT‑GGCAAAACATTCCTGCCGAGCGGCTTCGCTCATGGTTGGATAAAATGAATAAATGGAGCAGGTTAAACCATTGTTCGCTTTTGGTAATTAATCCCGAAAATAATAACGATAAACAATTTTCATTGTTGCTTGAGGAATAACGTTc                                                                                                                                                                                      >  2:302970/1‑250 (MQ=255)
                       cAATGCTAAATCATGAAAAAGGTCTATACTATTTGACCCGTGATTTGCAGTGTTCTATTGATCCCCATAATTACCTTTTTATTCTTGTTTGCGCAAATAACGCAT‑GGCAAAACATTCCTGCCGAGCGGCTTCGCTCATGGTTGGATAAAATGAATAAATGGAGCAGGTTAAACCATTGTTCGCTTTTGGTAATTAATCCAGAGAATAATAACGATAAACAATTTTCATTGTTGCTTGAGGAATACCGTTc                                                                                                                                                                                      >  2:398101/1‑250 (MQ=255)
                                  aaTGAAAAAAGTCTATACGAGTTGACCCGTGATTTGCAGTGTACTATTGATCCCCATAAGTACCTTTTTATTCTTGTTTGCGCAAATAACGCAG‑AGCAAAACATTCCTGCCGAGCGGATTCGCTCATGGTTGGATAAAATGAATAAATGGAGCAGGGTAAACCATTGTTCGCTTTTGGTAATTAATCCCGAAAATAATAACGATAAACAATTTTCATTGGTGCGTGAGGAATTCCGTTCTCTTTTTGGTc                                                                                                                                                                           <  2:202490/249‑1 (MQ=255)
                                    tGAAAAAGGTCTATACTATTTGACCCGTGATTTGCAGTGTTCTATTGATCCCCATAATTACCTTTTTATTCTTGTTTGCGCAAATAACGCAT‑GGCAAAACATTCCTGCCGAGCGGCTTCGCTCATGGTTGGATAAAATGAATAAATGGAGCAGGTTAAACCATTGTTCGCTTTTGGTAATTAATCCCGACAATAATAACGATAAACAATTTTCATTGTTGCTTGAGGAATACCGTTCACTTTTTGGTCTTg                                                                                                                                                                        >  1:314446/1‑251 (MQ=255)
                                       aaaaGGTCGATACTAGTTGAACCGAGGTTTGCAGTGTTCGATGGGTGCCCATTATTACATTTTTATTCTTGTTTGCGCTAATAACGCAT‑GGCAAAACATTCATGCCGAGCGGCTTCGCTCATGGTTGGATAAAATGAATAAATGGAGAAGGTTCACCAATTGTTCGCTTTTGGTAATTAATCCCGACAATACTAACAATAAACAATTTTCATTGTTGCTTGTGTAATACCATTCaattatagatcatgccg                                                                                                                                                                     >  2:168779/1‑235 (MQ=255)
                                                               tGATATGCAGTGTTCTATTGATCCCCATAATTACCTTTTTATTCTTGTTTGCGCAAATCACGCAT‑CGCAAAACATTCCTGCCGAGCGGCTTCGCTCATGGTTGGATAAAATGAATAAATGGAGCAGGTTAAACCATTGATCGCTTTTGGTAATTAATCCCGACAATAATAACGTTAAACAATTTTCATTGTTGCTTGTGGAATACCGTACACTTTTTGGACTTGCCACTTTGCGTTTTCCGGGCGACCAAc                                                                                                                                             >  2:128968/1‑251 (MQ=255)
                                                                    ttccatgtTCTATTGATCCACAAAATTACCTTTGTATTCTTGTTTGCGCAAATAACG‑ATAGGAAAAACATTCCTGCAGAGCGGCTTCGCTCATGGTTTGATAAAATGAATAAATGGAGCAGGTTAAACCATTGGTCGTTTTTGGTAATTAATCCCGAAAATAATAACGATAAGCAATTTTCATTGTTGCTTGAGGAATACCGTTCCCTGTTTGGTCTTGCCAGTTTGCGTTGTCAGGGTGACTAACAttt                                                                                                                                         <  2:170506/245‑1 (MQ=255)
                                                                     gCAGGGTTCTATTGATCCCCATAATTACCTTTTTATTCTTGTTTTCGCAAATAACGCAT‑GGCAAAACATTCCTGCCGAGCGGCTTCGCTCATGGTTGGATAAAATGAATAAATGGAGCAGGTTAAACCATTGTTCGCTTTTGGTAATTAATCCCGAAAATAATAACGATAAACAATTTTCATTGTTGCTTGAGGAATACGGTTCACTTTTTGGTCTTGCCAGTTTGCGTTTTCAGGGTGACCAACATTTGc                                                                                                                                       <  2:258502/251‑1 (MQ=255)
                                                                          gtTCTATTGATCCCCATAATTACCTTTTTATTCTTGTTTGCGCAAATAACGCAT‑GGCAAAACATTCCTGCCGAGCGGCTTCGCTCATGGTTGGATAAAATGAATAAATGGAGCAGGTTAAACCATTGTTCGCTTTTGGTAATTAATCCCGAAAATAATAACGATAAACAATTTTCATTGTTGCTTGAGGAATACCGTTCACTTTTTGGTCTTGCCAGTTTGCGTTTTCAGGGTGACCAACATTTGCTGGa                                                                                                                                   <  1:64954/250‑1 (MQ=255)
                                                                                 tGATCCCCATAATTACCTTTTTATTCTTGTTTGCGCAAATAACGCAT‑GGCAAAACATTCCTGCCGAGCGGCTTCGCTCATGGTTGGATAAAATGAATAAATGGAGCAGGTTAAACCATTGTTCGCTTTTGGTAATTAATCCCGAAAATAATAACGATAAACAATTTTCATTGTTGCTTGAGGAATACCGTTCACTTTTTGGTCTTGCCAGTTTGCGTTTTCAGGGTGACCAACATTTGCTGGATATTGCCt                                                                                                                           >  1:116966/1‑251 (MQ=255)
                                                                                     ccccTTAATTCCCTTTATATGCTTGTTTGCGCAAATAACGCAT‑GTCAAAACATTCCTGCCGAGCGGCTTCGCTCATTGTTTGATAAAATGAATAAATGGAGCAGGTTAAACCATTGTTCGCTTTTGGTAATTAATCCCGAAAATAATAACGATAAACAATTTTCATTGTTGCTTGAGGAATACCGTGCACTTTTTGGTCTTGCCAGTTTGCGGTTTCAGGGTGACCACCATTTGCTTGATATTGCCTTCTg                                                                                                                       <  2:251160/251‑1 (MQ=255)
                                                                                                ccTTTTTATTCTTGTTTGCGCAAATAACGCAT‑GGCAAAACATTCCTGCCGAGCGGCTTCGCTCATGGTTGGATAAAATGAATAAATGGAGCAGGTTAAACCATTGTTCGCTTTTGGTAATTAATCCCGAAAATAATAACGATAAACAATTTTCATTGTTGCTTGAGGAATACCGTTCACTTTTTGGTCTTGCCAGTTTGCGTTTTCAGGGAGACCAACATTTGCTGGATATTGCCTTCTGGTGCAACGac                                                                                                             >  2:114999/1‑249 (MQ=255)
                                                                                                           ttGTTTGCGCAAATAACGCAT‑GGCAAAACATTCCTGCCGAGCGGCTTCGCTCATGGTTGGATAAAATGAATAAATGGAGCAGGTTAAACCATTGTTCGCTTTTGGTAATTAATCCCGAAaataat                                                                                                                                                                                                                               >  1:86337/1‑125 (MQ=255)
                                                                                                           ttGTTTGCGCAAATAACGCAT‑GGCAAAACATTCCTGCCGAGCGGCTTCGCTCATGGTTGGATAAAATGAATAAATGGAGCAGGTTAAACCATTGTTCGCTTTTGGTAATTAATCCCGAAaataat                                                                                                                                                                                                                               <  2:86337/125‑1 (MQ=255)
                                                                                                                  cgcAAATAACGCAT‑GGCAAAACATTCCTGCCGAGCGGCTTCGCTCATGGTTGGATAAAATGAATAAATGGAGCCGGTTAAACCATTGTTCGCTTTTGGCAATTAATCCCGAAAATAATAACGATAAACAATTTTCATTGTTGCTTGAGGAATAACGTTCACTTTTTGGTCTTGCCAGTTTGCGTTTTCAGGGTGACCAACATTTGCTGGATATTGCCTTCTGGTGCAACGAAACAGGGGTCAGCGCCCGTc                                                                                          >  1:452548/1‑251 (MQ=255)
                                                                                                                  cgcAAATAACGCAT‑GGCAAAACATTCCTGCCGAGCGGCTTCGCTCATGGTTGGATAAAATGAATAAATGGAGCAGGTTAAACCATTGTTCGCTTTTGGTAATTAATCCCGAAAATAATAACGATAAACAATTTTCATTGTTGCTTGAGGAATACCGTTCACTTTTTGGTCTTGCCAGTTTGCGTTTTCAGGGTGACCAACATTTGCTGGATATTGCCTTCTGGTGCAACGAAAAAGGGGTCAGCGCCCGTc                                                                                          >  1:331303/1‑251 (MQ=255)
                                                                                                                                               gCCGAGCGGCTTCGCTCATGGTTGGATAAAATGAATAAATGGAGCAGGTTAAACCATTGTTCGCTTTTGGTAATTAATCCCGAAAATAATAACGATAAACAATTTTCATTGTTGCTTGAGGAATACCGTTCACTTTTTGGTCTTGCCAGTTTGCGTTTTCAGGGTGACCAACATTTGCTGGATATTGCCTTCTGGTGCAACGAAAAAGGGGTCAGCGCCCGTCAGCAGCTTAGCGTTCAGCAACAAAATgg                                                              >  1:298549/1‑251 (MQ=255)
                                                                                                                                                           cgtgcATGGTTGGATAAAATGAATAAATGGAGCAGGTTAATACATTGTTCGCTTTTGGTAATTAATCCCGAAAATAATAACGATAAACAAGTTTCATTGTTGCTTGAGGAATACCGTTCACTTTTTGGTCTTGCCAGTTTGCGTTTTCAGGTTGACCAACATTTGCTGGATATTGCCTTCTGGTGCAACGAAAAAGGGGTCAGCGCCCGTCAGCAGCTTAGCGTTCAGCAACAAAATGGTATCTGGACAtt                                                  <  2:173762/247‑1 (MQ=255)
                                                                                                                                                           cgcgtaTGGTTGGATAAAATGAATAAATGGAGTAGGTTAAACCATTGTTCGCTTTTGGTAATTAATCCCGGAAATAATAACGATAAACAATTTTCATTGTTGCTTGAGGAATACCGTTCACTTTTTGGTCTTGCCAGTTTGCGTTTTCAGGGTGACCAACATTTGCTGGATATTGCCTTCTGGTGCAACGAAAAAGGGGTCAGCGCCCGTCAGCAGCTTAGCGTTCAGCAACAAAATGGTATCTGGACAt                                                   <  2:227472/245‑1 (MQ=255)
                                                                                                                                                                  ggTTGGATAAAATGAATAAATGGAGCAGGTTAAACCATTGTTCGCTTTTGGTAATTAATCCCGAAAATAATAACGATAAACAATTTTCATTGTTGCTTGAGGAATACCGTTCACTTTTTGGTCTTGCCAGTTTGCGTTTTCAGGGTGACCAACATTTGCTGGATATTGCCGTCTGGTGCAACGAAAAAGGGGTCAGCGCCCGTCAGCAGCTTAGCGTTCAGCAACAAAATGGTATCTGGACATTAGTTCa                                            <  2:202993/250‑1 (MQ=255)
                                                                                                                                                                   gTTGGATAAAATGAATAAATGGAGCAGGTTAAACCATTGTTCGCTTTTGGTAATTAATCCCGAAAATAATAACGATAAACAATTTTCATTGTTGCTTGAGGAATACCGTTCACTTTTTGGTCTTGCCAGTTTGCGTTTTCAGGGTGACCAACATTTGCTGGATATTGCCTTCTGGTGCAACGAAAAAGGGGTCAGCGCCCGTCAGCAGCTTAGCGTTCAGCAACAAAATGGTATCTGGACATTAGTTCaa                                           >  1:267729/1‑250 (MQ=255)
                                                                                                                                                                             attaaaaaaatgAGCAGGTTAAACTATTGTTCTCTTTTGTTAATGAATCCCGAAAATAACAACGATATACAATTTGCATTTTTTCTTGAGGAATACCGTGCTCTTTTTGGTCTTGCCAGTTTGCGTTTTCAGGGTGACCAACATTTGCTGGATATTGCCTTCTGGTGCAACGAAAAAGGGGTCTGCGCCCGTCAGCAGCTTAGCGTTCAGCAACAAAATGGTATCTGGACATTAGTTCAAAGCGAAGAGGc                                <  2:2118/240‑1 (MQ=255)
                                                                                                                                                                                aaTAAATGGAGCAGGTTAAACCATTGTTCGCTTTTGGTAATTAATCCCGAAAATAATAACGATAAACAATTTTCATTGTTGCTTGAGGAATACCGTTCACTTTTTGGTCTTGCCAGTTTGCGTTTTCAGGGTGACCAACAGTTGCTGGATATTGCCTTCTGGTGCAACGAAAAAGGGGTCAGCGCCCGTCAGCAGCTTAGCGTTCAGCAACAAAATGGTATCTGGACATTAGTTCAAAGCGAAGAGGCGga                             <  2:89485/251‑1 (MQ=255)
                                                                                                                                                                                   aaaTGGAGCAGGGTAAACCATTGTTCGCTTTTGGTAATTAATCCCGAAAATAATAACGATAAACAATTTTCATTGTTGCTTGAGGAATACCGTTCACTTTTTGTTCTTGCCAGTTTGCGTTTTCAGGGTGACCAACATTTTCTGGATATTGCCTTCTGGTGCAACGAAAAAGGGGTCAGCGCCCGTCAGCAGCTTAGCGTTCAGCAACAAAATGGTATCTGGACATTAGTTCAAAGCGAAGAGGCGgaga                           <  2:428031/250‑1 (MQ=255)
                                                                                                                                                                                            aGGTTAAACCATTGTTCGCTTTTGGTAATTAATCCCGAAAATAATAACGATAAACAATTTTCATTGTTGCTTGAGGAATACCGTTCACTTTTTGGTCTTGCCAGTTTGCGTTTTCAGGGTGACCAACATTTGCTGGATATTGCCTTCTGGTGCAACGAAAAAGGGGTCAGCGCCCGTCAGCAGCTTAGCGTTCAGCAACAAAATGGTATCTGGACATTAGTTCAAAGCGAAGAGGCGGAGATCCAACCACg                 <  2:267729/251‑1 (MQ=255)
                                                                                                                                                                                                         gTTCGCTTTTGGTAATTAATCCCGAAAATAATAACGATAAACAATTTTCATTGTTGCTTGAGGAATACCGTTCACTTTTTGGTCTTGCCAGTTTGCGTTTTCAGGGTGACCAACATTTGCTGGATATTGCCTTCTGGTGCAACGAAAAAGGGGTCAGCGCCCGTCAGCAGCTTAGCGTTCAGCAACAAAATGGTATCTGGACATTAGTTCAAAGCGAAGAGGCGGAGATCCAACCACGCAGCGACGaaaa     <  2:108810/250‑1 (MQ=255)
                                                                                                                                                                                                           tCGCTTTTGGGGATTAATCCCGAAAATGATAACGATAAACTATTTTCATTGATGCATTAGGCATACCATTCCCTTTTGGGTCTTGCCCGGTTGCGTTTTCAGAGTGACCAACATTTGCTGGATATTGCCTTCTGGAGCACCGAAAAAGGGGTCAGCGCCCGTAAGCGGCTTAGCGTTCAGCAACAAAATGGTATCTGGACATTAGATCAAAGCGAAGAGGAGAAAATCCACCAACGCAGCGACGAACAACg  >  2:53778/1‑251 (MQ=255)
                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                    
GCCGGAAAAAATAAAATTATTTTCAATGCTAAATCATGAAAAAGGTCTATACTATTTGACCCGTGATTTGCAGTGTTCTATTGATCCCCATAATTACCTTTTTATTCTTGTTTGCGCAAATAACGCAT‑GGCAAAACATTCCTGCCGAGCGGCTTCGCTCATGGTTGGATAAAATGAATAAATGGAGCAGGTTAAACCATTGTTCGCTTTTGGTAATTAATCCCGGAAATAATAACGATAAACAATTTTCATTGTTGCTTGAGGAATACCGTTCACTTTTTGGTCTTGCCAGTTTGCGTTTTCAGGGTGACCAACATTTGCTGGATATTGCCTTCTGGTGCAACGAAAAAGGGGTCAGCGCCCGTCAGCAGCTTAGCGTTCAGCAACAAAATGGTATCTGGACATTAGTTCAAAGCGAAGAGGCGGAGATCCAACCACGCAGCGACGAAAAACG  >  NC_000913/3696685‑3697137

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑