Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 64,716 C→T R355R (CGG→CGA)  polB ← DNA polymerase II

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00091364,7160CT83.3% 19.3 / ‑2.8 13R355R (CGG→CGA) polBDNA polymerase II
Reads supporting (aligned to +/- strand):  ref base C (2/0);  new base T (6/4);  total (9/4)
Fisher's exact test for biased strand distribution p-value = 5.15e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.75e-01

AGAAAGGTGCGGATGATCGACGGGTACAGGCTTTTATAGTCCAGCACCAGCACTGAATCATAAAGCCCTGGCCGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGCGGCGGCACTTCGCCGAGATTAGGCGCGACATAACCAGCGCGATGCATTCGCGGAAAATAGAGATGACCAAATGCCGCCACCGAACCGCCGTGTCGGTCCACCGGCAGGCCGTTCACCGTTGCCCGTTCGAGTAAAAATGGCATGATTTCAGTTTTGTGGAAGATCTGCGTCACCAGCT‑CGCAATCTTTCAGGTTATAAGTTGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGGTCAATTTCGTCCATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAGCTCCTGAGCGACAGTTTCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCT  >  NC_000913/64486‑64945
                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                      
aGAAAGGTGCGGATGATCGACGGGTACAGGCTTTTATAGTCCAGCACCAGTACCGAATCATAAAGCCCTGGCCGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGTGGCGGCACTTCGCCGAGATTAGGCGCGACATAACCAGCGAGGTGAATACGAGGAAAATAGAGATGACCAAACGCCGCCACCGATCCCCCGGGGCGGGCTACCGGCAGGCCGGTCACCGTCGGCCGTTCGAGGAAAAAAGGCAt                                                                                                                                                                                                                    >  2:41186/1‑251 (MQ=255)
                                tttaaagtccggacccagtaccgAATCATAAAGCCCGGGCCGTGAATCCATCACGTGGCCGCCAGGGCTGGCGTGGGGCGGCAATTCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGGTCGAGTAAAAATGGCATGATTTCAGTGTTATGGAAAATCTGCGTCAcc                                                                                                                                                                                     <  2:220103/228‑1 (MQ=255)
                                              ccagtaccgAATCATAAAGCCCTGGCCGTGAATCCATCAAGTAGCCGCCAGGGCTGGCGTGTGGCGGCACTTCGCCGAGATTAGGCGCGACATAACCCGCGCGGTGCATACGAGGAAAATAGCGATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCAACCGGCAGGCCGTTCACCGTCGATCGTTAGAGTAAAAATGGCATGATTTCAGTTTTATGGGAAATCTGAGTCACCAGCT‑CGCAATCTTTc                                                                                                                                                                     >  1:324921/9‑251 (MQ=255)
                                                             aaaGCCCTGGCCGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGTGGCGGCACTTCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGGGTAAAAATAGCCTGATTTCCGTTTTATGGAAAATCTTCGTCACCAGCT‑CGCAATCTTTTCGGTTTTAAGTCGcc                                                                                                                                                      >  2:45512/1‑251 (MQ=255)
                                                               aGCCCGGGCCGTGAATCCATCCCGTAGCCGCCAGGGCTGTCGTGTGGCGGCACTTCGCCGAGAGCAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTGCGTCACCAGCT‑CGCAATCTTTCAGGTTATAAGTCGCCAg                                                                                                                                                    <  2:174818/251‑1 (MQ=255)
                                                                        cGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGTGGCGGCACTTCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTGCGTCACCAGCT‑CGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGttt                                                                                                                                           <  2:451883/251‑1 (MQ=255)
                                                                          tGAATCCATCACGTAGCCGCCAGGGCTGGCGTGTGGCGGCACTTCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCAACCGGCAGGCCGGTCACCGTCGGTCCTTCGGGTGAAAATGGCAGTATTTCAGTTTTATGGAAAATTTGCGTCACCATCC‑CGCTCACTTTCAGGCTATAAGtaccccacgccggtttat                                                                                                                                         >  2:371396/1‑234 (MQ=255)
                                                                                        aGCCGCCAGGGCTGGCGTGTGGCGGCACTTCGCCGGGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTGCGTCACCAGCT‑CGCCATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAAcgg                                                                                                                           >  1:242779/1‑251 (MQ=255)
                                                                                          ccgccAGGGCTGGCGTGTGGCGGCACTTCGCCGAGATTAGGCGCGACATAACCAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCACCGATCCCCCGTGGCGGGCTACCGGCAGGGCGTTCACCGTCGCACGTTCGAGTAAAAATTGCATGATTTTAGTTTTATGGGAAATATTCGTCCACAGCTACG‑AAACTTTCAGGTTATCAGTCGCCCGCGCAGGTTTTTATTTGGCGAAAcggcg                                                                                                                         >  2:248861/1‑251 (MQ=255)
                                                                                                                                              cAGCGCGGTGCATACGAGGAAAATAGAGATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGAATGATTTCAGTTTTATGGCAAATCTGCGTCACCAGCT‑CGCAATCTTTCAGGTTATAAGTCACCAGCGCAGGTTTATCTTCGGCGAAACGGCGGTAAATTTTGTCCAATCGGACCCCCGGGTTATCGAGAGATTTTTCTTTGccc                                                                     >  1:421477/1‑250 (MQ=255)
                                                                                                                                                             gaggAAAATAGAGATGACCAAACGCCGCCACCGATCCCCCGTGGCGGTCTACCGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTGCGTCACCAGCT‑CGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGGTCAATTTCGTCCATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAACTCCTGAGCg                                                      <  2:276758/249‑1 (MQ=255)
                                                                                                                                                                                                 cgccgTGTCGGTCCACCGGCAGGCCGTTCACCGTTGCCCGTTAGCGTAAAAATGGCATGATTTCAGTTTTGTGGAGGATCTGCGTCACCAGCT‑CGCAATCTTTCAGGTTATAAGTTGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGGTCAATTTCGGCCATTCGGTCCCACGGGGTATCGATTGATTTTCCTTCGCCTAATAGCTCCTGCGGGACAGGTTCCAGCGAGAaagaaggataactccagaa                   >  2:43893/1‑237 (MQ=255)
                                                                                                                                                                                                                 cGGCAGGCCGTTCACCGTCGCTCGTTCGAGTAAAAATGGCATGATTTCAGTTTTATGGAAAATCTGCGTCACCAGCT‑CGCAATCTTTCAGGTTATAAGTCGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGGCCAATTTCGTCCCTTCGATCCCCCGGGTGTTCGATAAATTTTCCTTTGCCTAATAACTCCTGGGCGACGGGTTCCCGAGAGAAAGAAGAGAAAATCCAAAAcgagggttttagacccc  >  2:246427/1‑236 (MQ=255)
                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                      
AGAAAGGTGCGGATGATCGACGGGTACAGGCTTTTATAGTCCAGCACCAGCACTGAATCATAAAGCCCTGGCCGTGAATCCATCACGTAGCCGCCAGGGCTGGCGTGCGGCGGCACTTCGCCGAGATTAGGCGCGACATAACCAGCGCGATGCATTCGCGGAAAATAGAGATGACCAAATGCCGCCACCGAACCGCCGTGTCGGTCCACCGGCAGGCCGTTCACCGTTGCCCGTTCGAGTAAAAATGGCATGATTTCAGTTTTGTGGAAGATCTGCGTCACCAGCT‑CGCAATCTTTCAGGTTATAAGTTGCCAGCGCAGGTTTATCTTCGGCGAAACGGCGGTCAATTTCGTCCATTCGATCCCACGGGTTATCGATAGATTTTCCTTCGCCTAATAGCTCCTGAGCGACAGTTTCCAGCGAGAATGAAGAGAAATTCCAGAACGCGGATTTCAGCGCCT  >  NC_000913/64486‑64945

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑