Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 66,263 C→T T96T (ACG→ACA)  araD ← L‑ribulose‑5‑phosphate 4‑epimerase AraD

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00091366,2630CT94.6% 95.0 / NA 37T96T (ACG→ACA) araDL‑ribulose‑5‑phosphate 4‑epimerase AraD
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (16/19);  minor base G (1/1);  total (17/20)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCCCATGCAAACGGGCCGTGGGAATGGACCAGAACGCCGGGCATTTGCGCTGCATCGATACCCTGTTTTTCAAAGGTTTCTACGATGACGTTACCGGTTTCCCACTCATATTCGCCGTTGATTTCTGCGTCGGTCATTTTGCGGGTGCAGGGAATGGTGCCGTAGAAATAGTCGGCG‑TGGGTGGTGCCGGTTGCTGGAATCGACTGACCCGCCTGCGCCCAGATGGTGGCGTGGCGCGAGTGCGTATGCACAATGCCGCCAATGGAGGGGAATGCCTGATAGAGCAGCCGGTGAGTTGGCGTGTCGGAGGAGGGCTTTTTCGTACCTTCAACCACTT‑CACCGGTTTCGATGCTAACCACGACCATATCGTCAGCGGTCATGACGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCTCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAG  >  NC_000913/66021‑66505
                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                   
gcccATGCAAATGGGCCGTGAGAATGGACCAGGACGCCGGGCATTTGCGCTGCATCGATACCTTGTTTTTCGAAGGTTTCTACGATGACGTTACCGGTTTCCCACTCATATTCACCGTTGATTTCTGCGTCGGTCATTTTGCGGGTGCAGGGAAGGGTGCCGTAGAAATAGTCGGCG‑TGGGTGGTGCCGGTGGCTGGAATCGTCTGGCCCGCCTGCGCCCAGATAGTGGCGTGGCGCGAGTGTGTGTGca                                                                                                                                                                                                                                              <  2:334298/249‑1 (MQ=255)
       aaaatgggCCGGGAGAATGGACCAGGACGCCGGGCATTTGCGCTGCATCGATTCCATGTTTTGCGACGGTTTCTACGATGACGTTACCGGTTTCCCACTCAAATTCTCCTGTGATTTCTGCGTCGGTCATTTCGCGGGTGCAGGGAATGGTGCCGTAGAAATAGTCGGCG‑TGGGTGGTGCCGTTTGCTGGAACCGACGGGTCCGCCTGCGCCCAGATAGTGGCGTGGCGCGAGTGTGTGTGCACAATgccg                                                                                                                                                                                                                                      <  1:71902/246‑1 (MQ=255)
               ccGTGAGAATGGACCAGGACGCCGGGCATTTGCGCTGCATCGATACCCTGTTTTTCGAAGGTTTCTACGATGACGTTACCGGTTTCCCACTCATATTCACCGTTGATTTCTGCGTCGGTCATTTTGCGGGTGCAGGGAATGGTGCCGTAGAAATAGTCGGCG‑TGGGTGGTGCCGGTTGCTGGAATCGACTGGCCCGCCTGCGCCCAGATAGTGGCGTGGCGCGAGGGTGTGTGCACAATGCCGCCCATgg                                                                                                                                                                                                                               >  1:260598/1‑250 (MQ=255)
                     gAATGGACCAGGACGCCGGGCATTTGCGCTGCATCGATGCCCTGTTTTTCGAAGGTTTCTACGATGACGTTACCGGTTTCCCACTCATATTCACCGTTGATTTCTGCGTCGGTCATTTTGCGGGTGCAGGGAATGGTGCCGTAGAAATAGTCGGCG‑TGGGTGGTGCCGGTTGCTGGAATCGACTGGCCCGCCTGCGCCCAGATAGTGGCGTGGCGCGAGTGTGTGTGCCCAATGCCGCCAATGGACGGGa                                                                                                                                                                                                                         >  1:418595/1‑250 (MQ=255)
                         ggACCAGGACGCCGGGCATTTGCGCTTCATCGATACCCTGTTTTTCGAAGGTTTCTACGATGACGTTACCGGTTTCCCACTCATATTCACCGTTGATTGCTGCGTCGGTCATTTTGCGGGTGCAGGGAATGGTGCCGTAGAAATAGTCGGCG‑TGGGTGGTGCCGGTTGCTGGAATCGACTGGCCCGCCTGCGCCCAGATAGTGGCGTGGCGCGAGTGTGTGTGCACAATGCCGCCAATGGACGGGAATGcc                                                                                                                                                                                                                    <  1:335307/251‑1 (MQ=255)
                                    ccGGGCATTTGCGCTGCATCGATACCCTGTTTTTCGAAGGTTTCTACGATGACGTTACCGGTTTCCCACTCATATTCACCGTTGATTTCTGCGTCGGTCATTTTGCGGGTGCAGGGAATGGTGCCGTAGAAATAGTCGGCG‑TGGGTGGTGCCGGTTGCTGGAATCGACTGGCCCGCCTGCGCCCAGATAGTGGCGTGGCGCGAGTGTGTGTGCACAAAGCCGCCAATGGACGGGAATGCCTGATAGagc                                                                                                                                                                                                           >  1:193923/1‑249 (MQ=255)
                                                 cTGCATCGATACCCTGTTTTTCGAAGGTTTCTACGGTGACGTTACCGGTTTCCCACTCATATTCACCGTTGATTTCTGCGTCGGCCATTTTGCGGGTGCAGGGAATGGTGCCGTAGAAATAGTCGGCG‑TGGGTGGTGCCGGTTGCTGGCATCGACTGGCCCGCCTGCGCCCAGATGGTGGCGAGGCGGGAGTGTGTGTGCACAATGCCGCCAATGTACGGGCATTCCTGATAGAGCAGTCGGTGAGTTggg                                                                                                                                                                                            >  1:259780/1‑250 (MQ=255)
                                                     aTCGATTCCCTGTTTTTCGAAGGTTTCTACGATGACTTTACCGGTTTCCCACTCATATTCACCGTTGATTTCTGCGTCGGTCATTTTGCGGGTGCAGGGAATGGTGCCGTAGAAATAGTCGGCG‑TGGGTGGTGCCGGTTGCTGGAATCGACTGGCCCGCCTGCGCCCAGATAGTGGCGTGGCGCGAGTGTGTGTGCACAATGCCGCCAATGGACGGGAATGCCTGATAGAGCAGTCGGTGAGTTGGCgtat                                                                                                                                                                                        <  1:128284/251‑3 (MQ=255)
                                                     aTCGATACCCTGTTTTTCGAAGGTTTCTACGATGACGTTACCGTTTTCCCACTCATATTCACCGTTGATTTCTGCGTCGGTCATTTTGCGGGTGCAGGGAATGGTGCCGTAGAAATAGTCGGCG‑TGGGTGGTGCCGGTTGCTGGAATCGACTGGCCCGCCTGCGCCCAGATAGTGGCGTGGCGCGAGTGTGTGGGCACAATGCCCCCAATGGACGGGAATGCCTGATAGAGGCGGCGGTGAGTTggggtat                                                                                                                                                                                        >  2:198964/1‑246 (MQ=255)
                                                        gATACCCTTTTTGTCGAAGTTTTCTACGATGCCTGTACCGTTTTCACACTCAGCTGCACCGTTGATTGCTGCGTCGGTTATTGTGCGGGTGCAGGGAATGGTGCCGTAGAAAGAGTCGGCG‑TGGGTGGTGCCGGTTGCTGGAATCGACTGGCCCGCCTGCGCCCAGATAGGGGCGTGGCGCGAGTGTGTGGGCACAATGCCGTCAATGGACTGGAATGCCTGATAGAGCAGTCGGTGAGTTGGCGTATCgt                                                                                                                                                                                     <  2:36664/251‑2 (MQ=255)
                                                                 tttttCGAAGGTTTCTACGATGACGTTACCGGTTTCCCACTCATATTCACCGTTGATTTCTGCGTCGGTCATTTTGCGGGTGCAGGGAATGGTGCCGTAGAAATAGTCGGCG‑TGGGTGGTGCCGGTTGCTGGAATCGACTGGCCCGCCTGCGCCCAGATAGTGGCGTGGCGCGAGTGTGTGTGCACAATGCCGCCAATGGACGGGAATGCCTGATAGAGCAGTCGGTGAGTTGGCGTATCGGGGGCGGGCt                                                                                                                                                                            >  1:429037/1‑251 (MQ=255)
                                                                        aaGGTTTCTTCGATGACGTTTCCGGTTTCCCACTCATATTCACCGTTGATTTCTGCGTCGTTCATTTTGCGGGTGCAGGGAATGGTGCCGTAGAAATAGTCGGCT‑TGGGTGGTGCCGGTTGCTGGAATCGACTGGCCCGCCTGCGCCCAGATAGTGGCGTGGCGCGAGTGTGTGTGCACAATGCCGCCAATGGACGGGAATGCCTGATAGAGCAGTCGGTGAGTTGGCGTATCGGAGGAGGGCTTTTTCgc                                                                                                                                                                     <  1:324286/251‑2 (MQ=255)
                                                                                 aCGATGACGTTACCGGTTTCCCACTCATATTCACCGTTGATTTCTGCGTCGGTCATTTTGCGGGTGCAGGGAATGGTGCCGTAGAAATAGTCGGCG‑TGGGTGGTGCCGGTTGCTGGAATCGACTGGCCCGCCTGCGCCCAGATAGTGGCGTGGCGCGAGTGTGTGTGCACAATGCCGCCAATGGACGGGAATGCCTGATAGAGCAGTCGGTGAGTTGGCGTATCGGAGGAGGGCTTTTTCGCACCTTCAAc                                                                                                                                                            >  1:385792/1‑251 (MQ=255)
                                                                                                                caccGTTGATTGCTGCGTCGTTCATTTGGCGGGGGCAGGGAATGGTTCCGTAGAAATAGTCGGCGGTGGG‑GGTGCCGGTTGCTGGAATCGCCTGGCCCGCCTGCGCCCAGATAGTGGCGTGGCGCGAGTGTGTGTGCACAATGCCGCCAATGGCCGGGAATGCCTGATAGAGCAGTCGGTGAGTTGGCGTATCGGAGGAGGGCTTTTTCGCACCTTCAACCACTT‑CACCGGTTTCGATGCTAACCACGAc                                                                                                                             <  2:170752/248‑1 (MQ=255)
                                                                                                                 accGTTGATTTCTGCGTCGGTCATTTTGCGGGTGCAGGGAATGGTGCCGTAGAAATAGTCGGCG‑TGGGTGGTGCCGGTTGCTGGAATCGACTGGCCCGCCTGCGCCCAGATAGTGGCGTGGCGCGAGTGTGTGGGCACAATGCCGCCAATGGACGGGAATGCCTGGTAGAGCAGTCGGTGAGGTGGCGTATCGGGGGAGGGCTTTTTAGCACCTTCAACCCCTT‑CACCGGTTTCGGTGCTAaccaccaccc                                                                                                                           >  2:90375/2‑250 (MQ=255)
                                                                                                                 accGTTGATTTCTGCGTCGGTCATTTTGCGGGTGCAGGGAATGGTGCCGTAGAAATAGTCGGCG‑TGGGTGGTGCCGGTTGCTGGAATCGACTGGCCCGCCTGCGCCCAGATAGTGGCGTGGCGCGAGTGGGTGTGCACAATGCCGCCAATGGACGGGAATGCCTGATCGAGCAGGCGGTGAGTTGGCGGATCGGGGGGGGGCTTTTTCGCACCTTCAACCACTT‑CCCCGGTTTCGATGCTAACCACGAcc                                                                                                                            >  2:420630/2‑250 (MQ=255)
                                                                                                                  ccGTTGGTTTCTGCGTCAGTCATTTTGCGGGTGCAGGGGATGATGCCGTAGAAATAGTCGGCG‑TGGGTGGTGCCGGTTGCTGGAATCGACTGGTCCGCCTGCGCCCAGATAGAGGCGTGGCGCGCGGGTGTGTGGACCATGCCGCCAATGGACGGGAATGCCTGATAGAGGAGTCGGTGAGGTGGCCTATCGGAGGAGGGGTTTTTCGCACCTTCAACCACTTTCACGGGTTT‑GATGcgaaacccggccc                                                                                                                           >  2:80916/1‑238 (MQ=255)
                                                                                                                  ccGTTGATTTCTGGGTCGGTCATTTTGCGGGGGCAGGGAATGGTGCCTTAGAAAGAGTCGGCG‑TGGGTGGTGCCGGTTGCTGGAAGCGACTGGCCCGCCTGCGCCCAGATAGTGGCGTGGCGCGAGTGTGTGTGCACAATGCCGCCAATGGACGGGAATGCCTGATAGAGCAGTCGGTGAGTTGGCGTATCGGAGGAGGGCTTTTTCGCACCTTCAACCACTT‑CACCGGTTTCGATGCTAACCACGACCat                                                                                                                          <  1:289008/251‑1 (MQ=255)
                                                                                                                       gATTTCTGCGTCGGTCATTTTGCGGGTGCAGGGAATGGTGCCGTAGAAATAGTCGGCG‑TGGGTGGTGCCGGTTGCTGGAATCGACTGGCCCGCCTGCGCCCAGATAGTGGCGTGGCGCGAGTGTGTGTGCACAATGCCGCCAATGGACGGGAATGCCTGATAGAGCAGTCGGTGAGTTGGCGTATCGGAGGAggg                                                                                                                                                                              >  1:443060/1‑195 (MQ=255)
                                                                                                                                       aTTTTGCGGGTGCAGGGAATGGTGCCGTAGAAATAGTCGGCG‑TGGGTGGTGCCGGTTGCTGGAATCGACTGGCCCGCCTGCGCCCAGATAGTGGCGTGGCGCGAGTGTGTGTGCACAATGCCGCCAATGGACGGGGATGCCTGATAGAGCAGTCGGTGAGTTGGCGTATCGGGGGAGGGCTTTTTCGCACCTTCAACCACTT‑CACCGGCTTCGATGCTAACCACGACCATATCGTCAGCGGTCATGATGCt                                                                                                     >  1:157203/1‑251 (MQ=255)
                                                                                                                                                   cAGGGAATGGTGCCGTAGAAATAGTCGGCG‑TGGGTGGTGCCGGTTGCTGGAATCGACTGGCCCGCCTGCGCCCAGATAGTGGCGTGGCGCGAGTGTGTGTGCACAATGCCGCCAATGGACGGGAATGCCTGATAGAGCAGTCGGTGAGTTGGCGTATCGGAGGAGGGCTTTTTCGCACCTTCAACCACTT‑CACCGGTTTCGATGCTAACCACGACCATATCGTCAGCGGTCATGATGCTGTAATCGACGcc                                                                                         <  1:124492/251‑1 (MQ=255)
                                                                                                                                                              gCCGTAGAAATAGTCGGCG‑TGGGTGGTGCCGGTTGCTGGAATCGACTGGCCCGCCTGCGCCCAGATAGTGGCGTGGCGCGAGTGTGTGTGCACAATGCCGCCAATGGACGGGAATGCCTGATAGAGCAGTCGGTGAGTTGGCGTATCGGAGGAGGGCTTTTTCGCACCTTCAACCACTT‑CACCGGTTTCGATGCTAACCACGACCATATCGTCAGCGGTCATGATGCTGTAATCGACGCCGGAAGGTTTGa                                                                              <  1:413474/251‑1 (MQ=255)
                                                                                                                                                              gCCGTAGAAATAGTCGGCG‑TGGGTGGTGCCGGTTGCTGGAATCGACTGGCCCGCCTGCGCCCAGATAGTGGCGTGGCGCGAGTGTGTGTGCACAATGCCGCCAATGGACGGGAATGCCTGATAGAGCAGTCGGTGAGTTGGCGTATCGGAGGAGGGCTTTTTCGCACCTTCAACCACTT‑CACCGGTTTCGATGCTAACCACGACCATATCGTCAGCGGTCATGATGCTGTAATCGACGCCGGAAGGTTTGa                                                                              <  1:198964/251‑1 (MQ=255)
                                                                                                                                                                    gAAATAGTCGGCG‑TGGGTGGTGCCGGTTGCTGGAATCGACTGGCCCGCCTGCGCCCAGATAGTGGCGTGGCGCGAGTGTGTGTGCACAATGCCGCCAATGGACGGGAATGCCTGATAGAGCAGTCGGTGAGTTGGCGTATCGGAGGAGGGCTTTTTCGCACCTTCAACCACTT‑CACCGGTTTCGATGCTAACCACGACCATATCGTCAGCGGTCATGATGCTGTAATTGACGCCGGAAGGTTTGATcaca                                                                         >  1:133066/1‑250 (MQ=255)
                                                                                                                                                                     aaaTAGTCGGCT‑TGGGTGGTGCCGGTTGCTGGAATCGACTGGCCCGCCTGCGCCCAGATAGTGGCGTGGCGCGAGTGTGTGTGCACAATGCCGCCAATGGACGGGAATGCCTGATAGAGCAGTCGGTGAGTTGGCGTATCGGAGGAGGGCTTTTTCGCACCTTCAACCACTT‑CACCGGTTTCGATGCTAACCACGACCATATCGTCAGCGGTCATGATGCTGTAATCGACGCCGGAAGGTTTGATCACaaa                                                                       <  1:379824/251‑1 (MQ=255)
                                                                                                                                                                                g‑tgggtggTGCCGGTTGTTGGATGCGACTGGCCCGCCTGCGCCCAGATAGTGGCGTGTCGCGAGGGTGTGTGCACAATGCCGCCAATGGACGGGAATGCCTGATAGAGCAGTCGGTGAGTTGGCGTATCGGAGGAGGGCTTTTTCGCCCCTTCAACCACTT‑CACCGGTTTCTATGCTAACCACGACCATATCGTCAGCGGTCATGATGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCt                                                            <  1:423751/251‑1 (MQ=255)
                                                                                                                                                                                         tgCCGGTTGCTGGAATCGACTGGCCCGCCTGCGCCCAGATAGTGGCGTGGCGCGAGTGTGTGTGCACAATGCCGCCAATGGACGGGAATGCCTGATAGAGCAGTCGGTGAGTTGGCGTATCGGAGGAGGGCTTTTTCGCACCTTCAACCACTT‑CACCGGTTTCGATGCTAACCACGACCATATCGTCAGCGGTCATGATGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGAAGCCGCGCTCGCGATc                                                     >  1:293713/1‑250 (MQ=255)
                                                                                                                                                                                                    gaaaccgcttggcccGCTTGCGCCCGGATAGTGGCGTGGCGTGAGTGTGTGTGCACAATGCCGCCAATGGACGGTAATGCCTGATAGAGCAGTCGGTGAGGTGGCGTATCGGAGGAGGGCTTTTTCGCAGTTTCAACCACTT‑CACCGTGTTCGATGCTAACCACGTCCATAGCGTCATCGCTCATGATGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCTCGCGATCAACGGCGCTGAc                                         <  1:427298/239‑1 (MQ=255)
                                                                                                                                                                                                            ctggcccGCCTGCGCCCAGATAGTGGCGTGGCGCGAGTGTGTGTGCACAATGCCGCCAATGGACGGGAATGCCTGATAGAGCAGTCGGTGAGTTGGCGTATCGGAGGAGGGCTTTTTCGCACCTTCAACCACTT‑CACCGGTTTCGATGCTAACCACGACCATATCGTCAGCGGTCATGATGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCTCGCGATCAACGGCGCTGACGTTGcccc                                 >  2:160563/5‑251 (MQ=255)
                                                                                                                                                                                                                   gCCTGCGCCCAGATAGTGGCGTGGCGCGAGTGTGTGTGCACAATGCCGCCAATGGACGGGAATGCCTGATAGAGCAGTCGGTGAGTTGGCGTATCGGAGGAGGGCTTTTTCGCACCTTCAACCACTT‑CACCGGTTTCGATGCTAACCACGACCATATCGTCAGCGGTCATGATGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCTCGCGATCAACGGCGCTGACGTTGCCCCATGTGAg                          <  1:41979/251‑1 (MQ=255)
                                                                                                                                                                                                                        cgcCCAGATAGTGGCGTGGCGCGAGTGTGTGTGCACAATGCCGCCAATGGACGGGAATGCCTGATAGAGCAGTCGGTGAGTTGGCGTATCGGAGGAGGGCTTTTTCGCACCTTCAACCACTT‑CACCGGTTTCGATGCTAACCACGACCATATCGTCAGCGGTCATGATGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCTCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGa                     <  1:160563/251‑1 (MQ=255)
                                                                                                                                                                                                                            cAGATAGTGGCGTGGCGCGAGTGTGTGTGCACAATGCCGCCAATGGACGGGAATGCCTGATAGAGCAGTCGGTGAGTTGGCGTATCGGAGGAGGGCTTTTTCGCACCTTCAACCACTT‑CACCGGTTTCGATGCTAACCACGACCATATAGTCAGCGGTCATGATGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCTCGCGCTCAACGGCGCTGACGTTTCCCCATGTGAGCGTGAcc                   >  1:88820/1‑249 (MQ=255)
                                                                                                                                                                                                                               atagtggcgtggcGCGAGTGTTGGTGCACAATGCCGCCAATGGACGGGAATGCCTGATAGAGCAGTCGGTGAGTTGGCGTATCGGAGGAGGGCTTTTTCGCACCTTCAACCACTT‑CACCGGTTTCGATGCTAACCACGACCATATCGTCAGCGGTCATGATGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCTCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGtt              <  1:438626/248‑1 (MQ=255)
                                                                                                                                                                                                                                       gtggcgCGAGGGGGTGTGCACAAGGCCGACAATGGACGGGAATGACTGATAGAGCAGTCGGTGAGTTGGCGTATAGGAGGAGGGCTTTTGCGCACCTTCAACCACTT‑CACCGGTTGCGATGCTAACCACGACCATATCGTCAGCGGTCATGATGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCTCGCGATCAACGGCGCTGACGTTGTCCCATGTGAGCGTGACCAGGTTATGTTTTg      <  1:185370/251‑1 (MQ=255)
                                                                                                                                                                                                                                         ggcgCGATTGTGTGTGCACAATGCCGCCAATGGACGGGAATGCCTGATAGAGCAGTCGGTGAGTTGGCGTATCGGAGGAGGGCTTTTTCGCACCTTCAACCACTT‑CACCGGTTTCGATGCTAACCACGACCATATCGTCAGCGGTCATGATGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCTCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTATGTTTTgg     <  1:114107/250‑1 (MQ=255)
                                                                                                                                                                                                                                           cgcgAGTGTGTGTGCCCAATGCCGCCAATGGACGGGAATGCCTGATAGAGCAGTCGGTTAGTTGGCGTATCGGAGGAGGGCTTTTTCGCACCTTCAACCACTT‑CACCGGTTTCGATGCTAACCACGACCATATCGTCAGCGGTCATGATGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCTCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTATGTTTTGGCAg  <  1:2956/251‑1 (MQ=255)
                                                                                                                                                                                                                                           cgagAGTGTGTGTGCACAATGCCGCCAATGGAAGGGAATGCCTGATAGAGCAGTCGGTGAGTTGGCGTATCGGAGGAGGGCTTTTTCGCACCTTCAACCACTT‑CACCGGTTTCGATGCTAACCACGACCATATCGTCAGCGGTCATGATGCTGTAATCGACGCCGGAAGGTTTGATCACAGAGACGCAGCGCTCGCGTTCAACGGCGCTGACGTTGCCCCATGGGAGCGTGACAAGGTTATGTTTTGGc    >  2:180700/4‑249 (MQ=255)
                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                   
CCCCATGCAAACGGGCCGTGGGAATGGACCAGAACGCCGGGCATTTGCGCTGCATCGATACCCTGTTTTTCAAAGGTTTCTACGATGACGTTACCGGTTTCCCACTCATATTCGCCGTTGATTTCTGCGTCGGTCATTTTGCGGGTGCAGGGAATGGTGCCGTAGAAATAGTCGGCG‑TGGGTGGTGCCGGTTGCTGGAATCGACTGACCCGCCTGCGCCCAGATGGTGGCGTGGCGCGAGTGCGTATGCACAATGCCGCCAATGGAGGGGAATGCCTGATAGAGCAGCCGGTGAGTTGGCGTGTCGGAGGAGGGCTTTTTCGTACCTTCAACCACTT‑CACCGGTTTCGATGCTAACCACGACCATATCGTCAGCGGTCATGACGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCTCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAG  >  NC_000913/66021‑66505

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑