Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,092,703	A→G	F41L (TTC→CTC)	frvA ←	putative PTS enzyme IIA component FrvA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,092,703	0	A	G	93.3%	122.0 / 0.7	46	F41L (TTC→CTC)	frvA	putative PTS enzyme IIA component FrvA
Reads supporting (aligned to +/- strand):  ref base A (2/1);  new base G (16/26);  total (18/28)
Fisher's exact test for biased strand distribution p-value = 5.55e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.18e-01

GAATAATTTTGCGACACAGTGTGCCGATGATTTTGACCTGATCCTCTTCGCCGCTTTGCGGCACGCCGAGGCAGATCCAGCAATTGACGTCTTCGCCATCGCTGGCTTTCCAGTCAATAGCCTGCGCTTTGCGGGCGAATAATACGAACGGTTGTTTAACGCAGGCGCTTTTACCGTGCGGCACGGCGACACCGGAACCAAATCCAGTAGAGTGCATTTTTTCGCGCAGCAGCAGGGTTTGCAGGAACTGGTGTGAGTCGGTGATAAAACCGTTTTGTAACGCTATTGTCGCCAACTGCTTCAGAACGGAATAAGCGCCATTGCCCTGAATATTCAGGTCAATACAGCTTGCAGTAAGAGCTGCCATTAATCGGACTCCATAAAATATATTTTAATCAATTGTAATGGTGGAGCGATTAAGTGATGAGAGGCGATATGTCGGTAAACAATGACAATTTGAGACAGAGTGAAAGGTCAGATTTGCGGAGTAATG  >  NC_000913/4092457‑4092949                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                       gAATAATTTTGCGACACAGTGTGCCGATGATTTTGACCTGATCCTCTTCGCCGCTTTGCGGCACGCCGAGGCAGATCCAGCAATTGACGTCTTCGCCATCGCTGGCTTTCCAGTCAATAGCCTGCGCTTTGCGGGCGAATAATACGAACGGTTGTTTAACGCAGGCGCTTTTACCGTGCGGCACGGCGACACCGGAACCAAATCCAGTAGAGTGCATTTTTTCGCGCAGCAGCAGGGTTTGCAGGAGCTgg                                                                                                                                                                                                                                                    >  1:370270/1‑251 (MQ=255)  aataatTTTGCGACACAGTGTGCCGATGATTTTGACCTGATCCTCTTCGCCGCTTTGCGGCACGCCGAGGCAGATCCAGCAATTGACGTCTTCGCCATCGCTGGCTTTCCAGTCAATGGCCTGCGCTTTGCGGGCGAATAATACGAACGGTTGTTTAACGCAGGCGCTTTTACCGTGCGGCACGGCGACACCGGAACCAAATCCAGTAGAGTGCATTTTTTCGCGCAGCAGCAGGGTTTGCAGGAGCTGgt                                                                                                                                                                                                                                                   <  1:396722/251‑1 (MQ=255)      atTTTGCGACACAGTGTGCCGATGATTTTGACCTGATCCTCTTCGCCGCTTTGCGGCACGCCGGGGCAGATCCAGCAATTGACGTCTTCGCCATCGCTGGCTTTCCAGTCAATAGCCTGCGCTTTGCGGGCGAATAATACGAACGGATGTTTAACGCAGGCGCTGTTACCGTGCGGCACGGCGACACCGGAACCAAATCCAGTAGAGTGCATTTTTTCGCGAAGCAGCAGGGTTTGCAGGCGCTGGtgtg                                                                                                                                                                                                                                                >  1:72064/1‑250 (MQ=255)        tttGCGACACAGTGTGCCGATGATTTTGACCTGATCCTCTTCGCCGCTTTGCGGCACGCCGAGGCAGATCCAGCAATTGACGTCTTCGCCATCGCTGGCTTTCCAGTCAATAGCCTGCGCTTTGCGGGCGAATAATACGAACGGTTGTTTAACGCAGGCGCTTTTACCGTGCGGCACGGCGACACCGGAACCAAATCCAGTAGAGTGCATTTTTTCGCGCAGCAGCAGGGTTTGCAGGAGCTGGTGTGAgg                                                                                                                                                                                                                                             >  1:52886/1‑250 (MQ=255)            cgacccagtggtccGATTATTTTGCCCTGATCCGCTTCGCCGCTTTGCGGCACGCCGAGGCAGATCCAGCAATTGACGGCGTCGCCATCGCTGGCTTTCCAGTCAATAGCCTGCGCTTTGCGGGCGAATAATACGAACGGTTGTTTAACGCAGGCGCTTTTACCGTGCGGCACGGCGACACCGGAACCAAATCCAGTAGAGTGCATTTTTTCGCGCAGCATCAGGGTTTGCAGGAGCTGGTGTGAGTCGGt                                                                                                                                                                                                                                         <  2:433163/239‑1 (MQ=255)               cacaGTGTGCCGATGATTTTGACCTGATCCTCTTCGCCGCTTTGCGGCACGCCGAGGCAGATCCAGCAATTGACGTCTTCGCCATCGCTGGCTTTCCAGTCAATAGCCTGCGCTTTGCGGGCGAATAATACGAACGGTTGTTTAACGCAGGCGCTTTTACCGTGCGGCACGGCGACACCGGAACCAAATCCAGTAGAGTGCATTTTTTCGCGCAGCAGCAGGGTTTGCAGGAGCTGGTGTGAGTCGGTGAt                                                                                                                                                                                                                                      <  1:87601/251‑1 (MQ=255)                  ggtgggCCGATGATTTTGACCTGCTCCTCTTCGCCGCTTTGCGGCACGCCGAGGCAGATCCAGCAATTGACGTCTTCGCCATCGCTGGCTTTCCAGTCAATAGCCTGCGCTTTGCGGGCGAATAATACGAACGGTTGTTTAACGCAGGCGCTTTTACCGTGCGGCACGGCGACACCGGAACCAAATCCAGTAGAGTGCATTTTTTCGCGCAGCAGCAGGGTTTGCAGGAGCTGGTGTGAGTCGGTGATaaa                                                                                                                                                                                                                                   <  2:98150/246‑1 (MQ=255)                     gtgCCGATGATTTTTGCCTGAGCCTCTTCGCCGCTTTCCGGCACGCCGAGGCAGATCCAGCAATTGACGTCTTCGCCATCGCTGGCTTTCCAGTCAATAGCCTGCGCTTTGCGGGCGAATAATACGAACGGTTGTTTAACGCAGGCGCTTTTACCGTGCGGCACGGCGACACCGGAACCAATTCCAGTAGAGTGTATTTTTTCGCGCAGCAGCAGGGTTTGCAGGAGCTGGTGTGAGTCGGTGATAAAAcc                                                                                                                                                                                                                                <  1:456745/251‑1 (MQ=255)                     gtgCCGATGATTTTGACCTGATCCTCTTCGCCGCTTTGCGGCACGCCGAGGCAGATCCAGCAATTGACGTCTTCGCCATCGCTGGCTTTCCAGTCAATAGCCTGCGCTTTGCGGGCGAATAATACGAACGGTTGTTTAACGCAGGCGCTTTTACCGTGCGGCACGGCGACACCGGAACCAAATCCAGTAGAGTGCATTTTTTCGCGCAGCAGCAGGGTTTGCAGGAGCTGGTGTGAGTCGGTGATAAAAcc                                                                                                                                                                                                                                >  1:16394/1‑251 (MQ=255)                      tgCCGATGATTTTGACCTGATCCTCTTCGCCGCTTTGCGGCACGCCGAGGCAGATCCAGCAATTGACGTCTTCGCCATCGCTGGCTTTCCAGTCAATAGCCTGCGCTTTGCGGGCGAATAATACGAACGGTTGTTTAACGCAGGCGCTTTTACCGTGCGGCACGGCGACACCGGAACCAAATCCAGTAGAGTGCATTTTTTCGCGCAGCAGCAGGGTTTGCAGGAACTGGTGTGAGTCGGTGCTAAAACCg                                                                                                                                                                                                                               >  1:74741/1‑251 (MQ=255)                          gatgatTTTGACCTGACCCTCTTCGCCGCTTTGCGGCACGCCGAGGCAGATCCAGCAATTGACGTCTTCGCCATCGCTGGCTTTCCAGTCAATAGCCTGCGCTTTGCGGGCGAATAATACGAACGGTTGTTTAACGCAGGCGCTTTTACCGTGCGGCACGGCGACACCGGAACCAAATCCAGTAGAGTGCATTTTTTCGCGCAGCAGCAGGGTTTGCAGGAGCTGGTGTGAGTCGGTGATAAAACCGtttt                                                                                                                                                                                                                           <  1:151422/251‑1 (MQ=255)                                    ccccgtcccccttggctgcTTTGCGGCACGCCGAGGCAGATCCAGCAATTGACGTCTTCGCCATCGCTGGCTTTCCAGTCAAGAGCCTGCGCTTTGCGGGCGAATAATACTAACGGTTGTTTAACGCAGGCGCTTTTACCGTGCGGCACGGCGACACCGGAACCAAATCCAGTAGAGTGCATTTTTTCGCGCAGCAGCAGGGTTTGCATGAGCTGGTGTGAGTCGGTTATTAAACCTTTTTGTAACGCTAt                                                                                                                                                                                                                 <  2:177629/234‑1 (MQ=255)                                                    cgcTTTGCGGCACGCCGAGGCAGATCCAGCAATTGACGTCTTCGCCATCGCTGGCTTTCAAGTCAATAGCCTGCGCTTTGCGGGCGAATAATACGAACGGTTGTTTAACGCAGGCGCTTTTACCGTGCGGCACGGCGACACCGGAACCAAATCCAGTAGAGTGCATTTTTTCGCGCAGCAGCAGGGTTTGCAGGAGCTGGTGTGAGTCGGTGATAAAACCGTTTTGTAACGCTATTGTCGCCAACTGCTTc                                                                                                                                                                                                 >  2:282214/1‑251 (MQ=255)                                                                          gATCCAGCAATTGACGTCTTCGCCATCGCTGGCTTTCCAGTCAATAGCCTGCGCTTTGCGGGCGAATAATACGAACGGTTGTTTAACGCAGGCGCTTTTACCGTGCGGCACGGCGACACCGGAACCAAATCCAGTAGAGTGCATTTTTTCGCGCAGCAGCAGGGTTTGCAGGAGCTGGTGTGAGTCGGTGATAAAACCGTTTTGTAACGCTATTGTCGCCAACTGCTTCAGAACGGAATAAGCGCCATTGc                                                                                                                                                                           <  1:49426/251‑1 (MQ=255)                                                                           aTCCAGCAATTGACGTCTTCGCCATCGCTGGCTTTCCAGTCAATAGCCTGCGCTTTGCGGGCGAATAATACGAACGGTTGTTTAACGCAGGCGCTTTTACCGTGCGGCACGGCGACACCGGAACCAAATCCAGTAGAGTGCATTTTTTCGCGCAGCAGCAGGGTTTGCAGGAGCTGGTGTGAGTCGGTGATAAAACCGTTTTGTAACGCTATTGTCGCCAACTGCTTCAGAACGGAATAAGCGCCATTGcc                                                                                                                                                                          >  2:278310/1‑251 (MQ=255)                                                                                      gACGGCTTCGCCAACGCTGGCTTTCCAGTCAATAGCCTGCGCTTTGCGGGCGAATAATACGAACGGTTGTTTAACGCAGGCGCTTTTACCGTGCGGCACGGCGACACCGGAACCAAATCCAGTAGAGGGCATTTTGTCGCGCAGCAGCAGGGTTTGCAGGAACTGGTGTGAGTCGGTGATAAAACCGTTTTGTAACGCTATTGTCGCCAACTGCTTCAGAACGGAATAATCGCAATTGCCCTTAATATTCa                                                                                                                                                               <  2:74741/251‑1 (MQ=255)                                                                                        cGTCTTCGCCATCGCTGGCTTTCCAGTCAATAGCCTGAGCTTTGCGGGCGAATCATACGAACGGTTGTTTAACGCAGGCGCTTTTACCGTGCGGCACGGCGACACCGGAACCAACTCCAGTAGAGTGCATTTTTTCGCGCAGCAGCAGGGTTTGCAGGAGCTGGTGTGAGTCGGTGATAACACCGTTTTGTAACGCTATTGTCGCCGACTGCTTCAGAACGGAGTAAGCGCCATTGCCCTGTATATTctgg                                                                                                                                                             >  2:317347/1‑248 (MQ=255)                                                                                         gTCTTCGCCATCGCTGGCTTTCCAGTCAATATCCTGCGCTTTGCGGGCGAATAAGACGATCGGTTGGTTAACGCAGGCGCTTTTACCGTGCGGCACGGCGACACCGGAACCAAAGCCAGTAGAGTGCATTTTTTCGCGCAGCAGCAGGGGTTTCAGTAGCTGGTGTGATTCGGTGATAAAACCGTTTTGTAACGCTATTGTCGCCAACTGCTTCATAACGGAAGAAGCGCCATTTCCCTGAATATTCAGGt                                                                                                                                                            <  1:102842/251‑1 (MQ=255)                                                                                          tCTTCGCCATCGCTGGCTTTCCAGTCAATAGCCTGCGCTTTGCGGGCGAATAATACGAACGGTTGTTTAAAGCAGGCGCTTTTACAGAGCGGCACGGCGACACCGGAACCAAATCCAGTCGAGTGCATTTTTTCGCGCAGCAGCAGGGTTTGCAGGAGCTGGTGTGAGTCGGTGATAAACCAGTTTTGTAACGCTCTTGGCGCCAACTGCTTCAGAACGGAATAAGCGCCATTGCCCTGCATCTTCAgggc                                                                                                                                                           >  2:110168/1‑249 (MQ=255)                                                                                                   gcGCTAGCTTTGCAGTCAAATGCCTGCGCTTTTCGGGCGAATTATACGAACGGTTGTTTAACGCAGGCGCTTGTACCGTTCGGCACGGCGACACCGGAACCTAATCCAGTAGAGTGCATTTTTTCGCGCAGCAGCAGGGTTTGCAGGAGCTGGTGTGAGTCGGTGATAAAACCGTTTTGTAACGCTATTGACGCCAACTGCTTCAGAACGGAATAAGCGCCATTGCACTGAATATTCAGGTCAATACAg                                                                                                                                                    <  2:127290/248‑1 (MQ=255)                                                                                                     gCTGGCTTTCCAGTCAATAGCCTGCGCTTTGCGGGCGAATAATACGAACGGTTGTTTAACGCAGGCGCTTTTACCGTGCGGCACGGCGACACCGGAACCAAATCCAGTAGAGTGCATTTTTTCGCGCAGCAGCAGGGTTTGCAGGAGCTGGTGTGAGTCGGTGATAAAACCGTTTTGTAACGCTATTGTCGCCAACTGCTTCAGAACGGAATAAGCGCCATTGCCCTGAATATTCAGGTCAATACAGCTTg                                                                                                                                                >  1:123916/1‑251 (MQ=255)                                                                                                        ggCTTTCCAGTCAATAGCCTGCGCTTTGCGGGCGAATAATACGAACGGTTGTTTAACGCAGGCGCTTTTACCGTGCGGCACGGCGACACCGGAACCAAATCCAGTAGAGTGCATTTTTTCGCGCAGCAGCAGGGTTTGCAGGAGCTGGTGTGAGTCGGTGATAAAACCGTTTTGTAACGCTATTGTCGCCAACTGCTTCAGAACGGAATAAGCGCCATTGCCCTGAATATTCAGGTCAATACAGCTTGc                                                                                                                                               >  1:124853/1‑249 (MQ=255)                                                                                                            ttCCAGTCAATAGCCTGCGCTTTGCGGGCGAATAATACGAACGGTTGTTTAACGCAGGCGCTTTTACCGTGCGGCACGGCGACACCGGAACCAAATCCAGTAGAGTGCATTTTTTCGCGCAGCAGCAGGGTTTGCAGGAGCTGGTGTGAGTCGGTGATAAAACCGTTTTGTAACGCTATTGTCGCCACCTGCTTCAGAACGGCATAAGCGCCATTGCCCTGAATATTCAGGTCAATACAGCTTGCAGTaa                                                                                                                                          >  2:126388/1‑250 (MQ=255)                                                                                                              ccAGTCAATAGCCTGCGCTTTGCGGGCGAATAATACGAACGGTTGGTTAACGCAGGCGCTTTTACCGTGCGGCACGGCGACACCGGAACCAAATCCAGTAGAGTGCATTTTTTCGCGCAGCAGCAGGGTTTGCAGGAGCTGGTGTGAGTCGGTGATAAAACCGTTTTGTAACGCTATTGTCGCCAACTGCTTCAGAACGGAATAAGCGCCATTGCCCTGAATATTCAGGTCAATACAGCTTGCAGTAagag                                                                                                                                       <  1:67247/251‑1 (MQ=255)                                                                                                                   cAATAGCCTTCGTGTTGCTGGCGAAGAATACGAACGGTTTTTGAACGCAGGCTCCTTTACCGTGCGGCACGGCGACACCGGATCCAAATCCAGTAGAGTGCATTTGTTCGCGCAGCAGCAGGGTTTGCAGGAGCTGGTGTGATTCGGTGATAAAACCTTTTTGTAACGCTATTGTCGCCTACTGCTTCAGAACGGAATAAGCGCCATTGCCATGAATATACAGGTCAATACTGCTTTCAGTAAGAGCTGct                                                                                                                                  <  2:257893/251‑2 (MQ=255)                                                                                                                       aGCCTGCGCTTTGCGGGCGAATAATACGAACGGTTGTTTAACGCAGGCGCTTTTACCTTGCGGCACGGCGACACCGGAACCAAATCCAGTAGAGTGCATTTTTTCGCGCAGCAGCAGGGTTTGCAGGAGCTGGTGTGAGTCGTTGATAAAACCGTTTTGTAACGCTATTGTCGCCAACTGCTTCAGAACGGAATAAGCGCCATTGCCCTGAATTTTCAGGTCAATACAGCTTGCAGTAAGAGCTGCCAtt                                                                                                                               <  1:36875/250‑1 (MQ=255)                                                                                                                             cgcTTTGCGGGCGAATAATACGAACGGTTGTTTAACGCAGGCGCTTTTACCGTGCGGCACGGCGACACCGGAACCAAATCCAGTAGAGTGCATTTTTTCGCGCAGCAGCAGGGTTTGCAGGAGCTGGTGTGAGTCGGTGATAAAACCGTTTTGTAACGCTATTGTCGCCAACTGCTTCAGAACGGAATAAGCGCCATTGCCCTGAATATTCAGGTCAATACAGCTTGCAGTAAGAGCTGCCATTAATCGGa                                                                                                                        <  1:282214/251‑1 (MQ=255)                                                                                                                                 ttGCGGGCGAATAATACGAACGGTTGTTTAACGCAGGCGCTTTTACCGTGCGGCACGGCGACACCGGAACCAAATCCAGTAGAGTGCATTTTTTCGCGCAGCAGCAGGGTTTGCAGGAGCTGGTGTGAGTCGGTGATAAAACCGTTTTGTAACGCTATTGTCGCCAACTGCTTCAGAACGGAATAAGCGCCATTGCCCTGAATATTCAGGTCAATACAGCTTGCAGTAAGAGCTGCCATGAATCGGACt                                                                                                                      <  2:92154/249‑1 (MQ=255)                                                                                                                                 ttGCGGGCGAATAATACCAACGGTTGTTTAACGCAGGCGCTTTTTCCGTGCGGCCCGGCGACACCGGAACCAAATCCAGTAGAGTGCATTTTTTCGCGCAGCAGCAGGGTTTTCAGGATCTGGTGTGAGTCGGTGATAAAACCGTTTTGTAACGCTATTGTCGCCAACTGCTGCAGAACGGAATAAGCGCCATTGCCGTGAATATTCAGGTCAATACAGCTTGCAGTAAGATCTGCCAGTAATCGGACTc                                                                                                                     <  2:244137/250‑1 (MQ=255)                                                                                                                                          aataatACGAACGGTTGTTTAACGCAGGCGCTTTTACCGTGCGGCACGGCGACACCGGGACCAAATCCAGTAGAGTGCATTTTTTCGCGCAGCAGCAGGGTTTGAAGGAGCTGGTGTGAGTAGGTGATAAAACCGTTTTGTAACGCTATTGTCGCCAACTGCTTCAGAACGGAATCAGCGCCATTGCCCTGAATATTCAGGTAAATACAGCTAGCAGTACGAGCTGCCATTACACGGACTCCATAAAatat                                                                                                           >  1:275333/1‑251 (MQ=255)                                                                                                                                                                   aGGCGCTTTTACCGTGCGGCACGGCGACACCGGAACCAAATCCAGTAGAGTGCATTTTTTCGCGCAGCAGCAGGGTTTGCAGGAGCTGGTGTGAGTCGGTGATAAAACCGTTTTGTAACGCTATTGTCGCCAACTGCTTCAGAACGGAATAAGCGCCATTGCCCTGAATATTCAGGTCAATACAGCTTGCAGTAAGAGCTGCCATTAATCGGACTCCATAAAATATATTTTAATCAATTGTAAtggtgg                                                                                    >  1:377515/1‑249 (MQ=255)                                                                                                                                                                       gcTTTTACCGTGCGGCACGGCGACACCGGAACCAAATCCAGTAGAGTGCATTTTTTCGCGCAGCAGCAGGGTTTGCAGGAGCTGGTGTGAGTCGGTGATAAAACCGTTTTGTAACGCTATTGTCGCCAACTGCTTCAGAACGGAATAAGCGCCATTGCCCTGAATATTCAGGTCAATACAGCTTGCAGTAAGAGCTGCCATTAATCGGACTCCATAAAATATATTTTAATCAATTGTAATGGTGGAGCGAt                                                                              >  2:191560/1‑251 (MQ=255)                                                                                                                                                                       gcTTTTACCGTGCGGCACGGCGACACCGGAACCAAATCCAGGAGAGTGCATTTTTTCGCGCAGCAGCAGGGTTTGCAGGAGCTGGTGTGAGTCGGTGATAAAACCGTTTTGTAACGCTATTGTCGCCAACTGCTTCAGAACGGAATAAGCGCCATTGCCCTGAATATTCAGGTCAATACAGCTTGCAGTAAGAGCTGCCATTAATCGGACTCCATAAAATATATTTTAATCAATTGTAATGGTGGAGCGAt                                                                              <  1:15698/251‑1 (MQ=255)                                                                                                                                                                                    ggCACGGCGACACCGGAACCAAATCCCGTAGAGTGCATTTTTTCGCGCAGCAGAAGGGTTTGCAGGAGCTGGTGTGAGTCGGTGATAAAACCGTTTTGTAACGCTATTGTCGCCAACTGCTTCAGAACGGa                                                                                                                                                                                         >  2:442185/1‑131 (MQ=255)                                                                                                                                                                                    ggCACGGCGACACCGGAACCAAATCCAGTAGAGTGCATTTTTTCGCGCAGCAGCAGGGTTTGCAGGAGCTGGTGTGAGTCGGTGATAAAACCGTTTTGTAACGCTATTGTCGCCAACTGCTTCAGAACGGa                                                                                                                                                                                         <  1:442185/131‑1 (MQ=255)                                                                                                                                                                                     gCACGGCGACACCGGAACCAAATCCAGTAGAGTGCATTTTTTCGCGCAGCAGCAGGGTTTGCAGGAGCTGGTGTGAGTCGGTGATAAAACCGTTTTGTAACGCTATTGTCGCCAACTGCTTCAGAACGGAATAAGCGCCATTGCCCTGAATATTCAGGTCAATACAGCTTGCAGTAAGAGCTGCCATTAATCGGACTCCATAAAATATATTTTAATCAATTGTAATGGTGGAGCGATTAAGTGATGAGAgg                                                                <  1:278310/251‑1 (MQ=255)                                                                                                                                                                                            gACACCGGAACCAAATCCAGTAGAGTGCCTTTTTTCGCGCAGCAGCAGGGTTTGCAGGAACTGGAGTGAGTCGGTGGTAAAACCGTTTTGTAACGCTATTGTCGCCAACTGCTTCAGAACGGAATAAGCGCCATTGCCCTGAATATTCAGGTCAATACAGCTTGCAGTAAGAGCTGCCATTAATCGGACTCCATACAATATATTTTAATCAATTGTAATGGTGGAGCGATTAAGTGATGAGAGGCGATATg                                                         >  2:48391/1‑251 (MQ=255)                                                                                                                                                                                               cccGGAACCAAATCCAGTAGAGTGCATTTTTTCGCGCAGCAGCAGGGTTTGCAGGAGCTGGTGTGAGTCGGTGATAAAACCGTTTTGTAACGCTATTGTCGCCAACTGCTTCAGAACGGAATAAGCGCCATTGCCCTGAATATTCAGGTCAATACAGCTTGCAGTAAGAGCTGCCATTAATCGGACTCCATAAAATATATTTTAATCAATTGTAATGGTGGAGCGATTAAGTGATGAGAGGCGATATGTCg                                                      <  1:206505/250‑1 (MQ=255)                                                                                                                                                                                                      ccAAATCCAGTAGAGTGCATTTTTTCGCGCAGCAGCAGGGTTTGCAGGAGCTGGTGTGAGTCGGTGATAAAACCGTTTTGTAACGCTATTGTCGCCAACTGCTTCAGAACGGAATAAGCGCCATTGCCCTGAATATTCAGGTCAATACAGCTTGCAGTAAGAGCTGCCATTAATCGGACTCCATAAAATATATTTTAATCAATTGTAATGGTGGAGCGAGTAAGTGATGAGAGGCGATATGTCGGTAAACa                                               <  1:361030/251‑1 (MQ=255)                                                                                                                                                                                                        aaagccAGTAGAGTGCATTGTTTCGCGCAGCAGCAGGGTTTGCAGGAGCTGGTGTGAGTCGGTGATAAAACCGTTTTGTAACGCTATTGTCGCCAACTGCTTCAGAACGGAATAAGCGCCAGTGCCCTGAATATTCAGATCAATACAGCTTGCAGTAAGAGCTGCCATTAATCTGACGCCATAAAATATATTTTAAGCAATTGTAATGGTGGAGCGATTAAGTGATGAGAGGCGATATGGCGGTAAACAAt                                             <  2:261752/247‑1 (MQ=255)                                                                                                                                                                                                                        aTTTTTTCGCGCAGCAGCAGGGTTTGCAGGAGCTGGTGTGAGTCGGTGATAAAACCGTTTTGTAACGCTATTGTCGCCAACTGCTTCAGAACGGAATAAGCGCCATTGCCCTGAATATTCAGGTCAATACAGCTTGCAGTAAGAGCTGCCATTAATCGGACTCCa                                                                                                                   >  1:355276/1‑165 (MQ=255)                                                                                                                                                                                                                        aTTTTTTCGCGCAGCAGCAGGGTTTGCAGGAGCTGGTGTGAGTCGGTGATAAAACCGTTTTGTAACGCTATTGTCGCCAACTGCTTCAGAACGGAATAAGCGCCATTGCCCTGAATATTCAGGTCAATACAGCTTGCAGTAAGAGCTGCCATTAATCGGACTCCa                                                                                                                   <  2:355276/165‑1 (MQ=255)                                                                                                                                                                                                                               cgcccagcaacagGGTTTGCAGGAGCTGTTTTGAATCGGTGATAAAACCGTTTTTTAACGCTATTGTCTCCAACTGCTTCAGAACGGAATAAGCGCCATTGCCCTGAATATTCAGGTCAATACAGCTTGCAGTAAGAGCTGCCATTAATCGGACTCCATAAAATATATTTTAATCAATTGTAATGGTGGAGCGATTAAGTGATGAGAGGCGATATGTCGGTAAACAAGGACAATTTGAGACAGACTGaaa                       <  2:212175/246‑1 (MQ=255)                                                                                                                                                                                                                                      cagcagGGTTTGCAGGAGCTGGTGTGAGTCGGTGATAAAACCGTTTTGTAACGCTATTGTCGCCAACTGCTTCAGAACGGAATAAGCGCCATTGCCCTGAATATTCAGGTCAATACAGCTTGCAGTAAGAGCTGCCATTAATCGGACTCCATAAAATATATTTTAATCAATTGTAATGGTGGAGCGATTAAGTGATGAGAGGCGATATGTCGGTAAACAATGACAATTTGAGACAGAGTGAAAGGTCAGAt               <  1:374472/251‑1 (MQ=255)                                                                                                                                                                                                                                                 gCAGGAGCTGGTGTGAGTCGGTGATAAAACCGTTTTGTAACGCTATTGTCGCCAACTGCTTCAGAACGGAATAAGCGCCATTGCCCTGAATATTCAGGTCAATACAGCTTGCAGTAAGAGCTGCCATTAATCGGACTCCATAAAATATATTTTAATCAATTGTAATGGTGGAGCGATTAAGTGATGAGAGGCGATATGTCGGTAAACAATGACAATTTGAGACAGAGTGAAAGGTCAGATTTGCGGAGTa     <  1:205202/250‑1 (MQ=255)                                                                                                                                                                                                                                                   aGGAGCTGGTGTGAGTCGGTGATAAAACCGTTTTGTAACGCTATTGTCGGCAACTGCTTCAGAACGGAATAAGCGCCATTGCCCTGAATATTCAGGTCAATACAGCTTGCAGTAAGAGCTGCCATTAATCGGACTCCATAAAATATATTTTAATCAATTGTAATGGTGGAGCGATTAAGTGATGAGAGGCGATATGTCGGTAAACAATGACAATTTGAGACAGAGTGAAAGGTCAGATTTGCGGAGTAATg  <  2:425782/251‑1 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                       GAATAATTTTGCGACACAGTGTGCCGATGATTTTGACCTGATCCTCTTCGCCGCTTTGCGGCACGCCGAGGCAGATCCAGCAATTGACGTCTTCGCCATCGCTGGCTTTCCAGTCAATAGCCTGCGCTTTGCGGGCGAATAATACGAACGGTTGTTTAACGCAGGCGCTTTTACCGTGCGGCACGGCGACACCGGAACCAAATCCAGTAGAGTGCATTTTTTCGCGCAGCAGCAGGGTTTGCAGGAACTGGTGTGAGTCGGTGATAAAACCGTTTTGTAACGCTATTGTCGCCAACTGCTTCAGAACGGAATAAGCGCCATTGCCCTGAATATTCAGGTCAATACAGCTTGCAGTAAGAGCTGCCATTAATCGGACTCCATAAAATATATTTTAATCAATTGTAATGGTGGAGCGATTAAGTGATGAGAGGCGATATGTCGGTAAACAATGACAATTTGAGACAGAGTGAAAGGTCAGATTTGCGGAGTAATG  >  NC_000913/4092457‑4092949

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑