| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 86,658 | A→G | P343P (CCA→CCG) | ilvI → | acetolactate synthase/acetohydroxybutanoate synthase, catalytic subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 86,658 | 0 | A | G | 94.0% | 145.3 / ‑0.9 | 50 | P343P (CCA→CCG) | ilvI | acetolactate synthase/acetohydroxybutanoate synthase, catalytic subunit |
| Reads supporting (aligned to +/- strand): ref base A (1/2); new base G (23/24); total (24/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.53e-01 | |||||||||||
GAAGCCAATATGACGATGCATAACGCGGATGTGATTTTCGCCGTCGGGGTACGATTTGATGACCGAACGACGAACAATCTGGCAAAGTACTGCCCAAATGCCACTGTTCTGCATATCGATATTGATCCTACTTCCATTTCTAAAACCGTGACTGCGGATATCCCGATTGTGGGGGATGCTCGCCAGGTCCTCGAACAAATGCTTGAACTCTTGTCGCAAGAATCCGCCCATCAACCACTGGATGAGATCCGCGACTGGTGGCAGCAAATTGAACAGTGGCGCGCTCGTCAGTGCCTGAAATATGACACTCACAGTGAAAAGATTAAACCGCAGGCGGTGATCGAGACTCTTTGGCGGTTGACGAAGGGAGACGCTTACGTGACGTCCGATGTC‑GGGCAGCACCAGATGTTTGCTGCACTTTATTAT‑CCATTCGACAAACCGCGTCGCTGGATCAATTCCGGTGGCCTCGGCACGATGGGTTT > NC_000913/86422‑86903 | gAAGCCAATATGACGATGCATAACGCGGATGTGATTTTCGCCGTCGGGGTACGATTTGATGACCGAACGACGAACAATCTGGCAAAGTACTGCCCAAATGCCACTGTTCTGCATATCGATATTGATCCTACTTCCATTTCTAAAACCGTGACTGCGGATATCCCGATTGTGGGGGATGCTCGCCAGGTCCTCGAACAACTGCTTGAACTCTTGTCGCAAGAATCCGCCCATCAACCGCTGGATGAGATCcg > 1:422759/1‑251 (MQ=255) aaGCCAATATGACGATGCATAACGCGGATGTGATTTTCGCCGTCGGGGTACGATTTGATGACCGAACGACGAACAATCTGGCAAAGTACTGCCCAAATGCCACTGTTCTGCATATCGATATTGATCCTACTTCCATTTCTAAAACCGTGACTGCGGATATCCCGATTGTGGGGGATGCTCGCCAGGTCCTCGAACAAATGCTTGAACTCTTGTCGCAAGAATCCGCCCATCAACCGCTGGATGAGATCcgc > 1:280088/1‑251 (MQ=255) ccAATTTGACGAGGCATAACGCGGATGTGATTTTCGCCGTCGGGGTACGATTTGATGACCGAACGACGAACAATCTGGCAAAGTACTGCCCAAATGCCACTGTTCTGCATATCGATATTGATCCTACTTCCATTTCTAAAACCGTGACTGCGGATATCCCGATTGTGGGGGATGCTCGCCAGGTCCTCGAACAAATGCGTGAACTCTTGTCGCAAGAATCCGCCCATCAACCGCTGGATGAGATCCGCGAc < 2:13802/251‑1 (MQ=255) atatGACGATGCATAACGCGGATGTGATTTTCGCCGTCGGGGTACGATTTGATGACCGAACGACGAACAATCTGGCAAAGTACTGCCCAAATGCCACTGTTCTGCATATCGATATTGATCCTACTTCCATTTCTCAAACCGTGACTGCGGATATCCCGATTGTGTGGGATGCTCGCCAGGTCCTCGAACAACTTCTTGCACTCTGGTCGCAAGAATCCGCCCATCAACCGCTGAATGAGCTCCGCGCCtgg > 2:198421/1‑251 (MQ=255) atGACGATGCATAACGCGGATGTGATTTTCGCCGTCGGGGTACGATTTGATGACCGAACGACGAACAATCTGGCAAAGTACTGCCCAAATGCCACTGTTCTGCATAGCGATATTGATCCTACTTCCATTTCTAAAACCGTGACTGCGGATATCCCGATTGTGGGGGATGCTCGCCAGGTCCTCGAACAAATGCTTGAACTCTTGTCGCAAGAATCCGCCCATCAACCGCTGGATGAGATCCGCGACtggt < 2:43713/250‑1 (MQ=255) aTAACGCGGATGTGATTTTCGCCGTCGGGGTACGATTTGATGACCGAACGACGAACAATCTGGCAAAGTACTGCCCAAATGCCACTGTTCTGCATATCGATATTGATCCTACTTCCATTTCTAAAACCGTGACTGCGGATATCCCGATTGTGGGGGATGCTCGCCAGGTCCTCGAACAAATGCTTGAACTCTTGTCGCAAGAATCCGCCCATCAACCGCTGGATGAGATCCGCGACTGGTGGCAGCAAAtt > 1:260936/1‑251 (MQ=255) cgGATGTGATTTTCGCCGTCGGGGTACGATTTGATGACCGAACGACGAACAATCTGGCAAAGTACTGCCCAAATGCCACTGTGCTGCATATCGATATGGATCCTACTTCCATTTCTAAAACCGTGACTGCGGATATCCCGATTGTGGGGGATGCTCGCCAGGTCCTCGAACAAATGCTTGAACTCTTGTCGCAAGAATCCGCCCAGCAACCGCGGGATGAGATCCGCGACTGGTGGCATCAAATTGAACAg < 1:21975/251‑1 (MQ=255) tgATTTTCGCCGTCGGGGTACGATTTGATGACCGAACGACGAACAATCTGGCACAGAACTGCCCAAATGCCACTGTTCTGCATATCGATATTGCTCCTACTTCCATTTCTAAAACCGTGACTGCGGATATCCCGATTGTGGGGGATGCTCGCCAGGTCCTCGAACAAATGCTTGAACTCTTGTCGCAAGAATCCGCCCATCCACCGCTGGATGAGATCCGCGACTGGTGGCAGCAAATTGAACAGTGgcgc > 1:34849/1‑251 (MQ=255) ttCGCCGTCGGGGTACGATTTGATGACCGAACGACGAACAATCTGGCAAAGTACTGCCCACATGCCACTGTTCTGCATATCGATATTGATCCTACTTCCATTTCTAAAACCGTGACTGCGGATATCCCGATTGTGGGGGATGCTCGCCAGGTCCTCGAACAAATGCTTGAACTCTTGTCGCACGAATCCGCCCATCAACCGCTGGATGAGATCCGCGACTGGTGGCAGCAAATTGAACAGTGGCGCGCTCg > 1:442950/1‑251 (MQ=255) cGCCGTCGGGGGACGATTTGATGACCGATCGACGAACAATCTGGCAAAGTACTGCCCAAATGCCACTGTTCTTCATATCGATATTGATCCTACTTCCATTGCTAAAACCGTGACTGCGGATATCCCGATTGTGGGGGATGCTCGCCAGGTCCTCGAACAAATGCTTGAACTCTTGTCGCAAGAATCCGCCCATCAACCGCTGGATGAGATCCGCGACTGGTGGCAGCAAATTGAACAGTGGCGCGCTCGt < 2:205669/250‑1 (MQ=255) gCCGTCGGGGTACGATTTGATGACCGAACGACGAACAATCTGGCAAAGTACTGCCCAAATGCCACTGTTCTGCATATCGATATTGATCCTACTTCCATTTCTAAAACCGTGACTGCGGATATCCCGATTGTGGGGGATGCTCGCCAGGTCCTCGAACAAATGCTTGAACTCTTGTCGCAAGAATCCGCCCATCAACCGCTGGATGAGATCCGCGACTGGTGGCAGCAAATTGAACAGTGGCGCGCTCGTc > 2:44042/1‑250 (MQ=255) gatgaCCGAACGACGAACAATCTGGCAAAGTACTGCCCAAATGCCACTGTTCTGCATATCGATATTGATCCTACTTCCATTTCTAAAACCGTGACTGCGGATATCCCGATTGTGGGGGATGCTCGCCAGGTCCTCGAACAAATGCTTGAACTCTTGTCGCAAGAATCCGCCCATCAACCGCTGGATGAGATCCGCGACTGGTGGCAGCAAATTGAACAGTGGCGCGCTCGTCAGTGCCTGAAATATGacac > 1:277764/1‑251 (MQ=255) cGAACGACGAACAATCTGGCAAAGTACTGCCCAAATGCCACTGTTCTGCATATCGATATTGATCCTACTTCCATTTCTAAAACCGTGACTGCGGATATCCCGATTGTGGGGGATGCTCGCCAGGTCCTCGAACAAATGCTTGAACTCTTGTCGCAAGAATCCGCCCATCAACCGCTGGATGAGATCCGCGACTGGTGGCAGCAAATTGAACAGTGGCGCGCTCGTCAGTGCCTGAAATATGACACTcac > 1:348565/1‑249 (MQ=255) gaACAATCTGGCAAAGTACTGCCCAAATGCCACTGTTCTGCATATCGATATTGATCCTACTTCCATTTCTAAAACCGTGACTGCGGATATCCCGATTGTGGGGGATGCTCGCCAGGTCCTCGAACAAATGCTTGAACTCTTGTCGCAAGAATCCGCCCATCAACCGCTGGATGAGATCCGCGACTGGTGGCAGCAAATTGAACAGTGGCGCCATCGTCAGTGCCTGAAATATGACACTCACAGTaaaa > 1:13513/1‑248 (MQ=255) cAATCTGGCAAAGTACTGCCCAAATGCCACTGTTCTGCATATCGATATTGATCCTACTTCCATTTCTAAAACCGTGACTGCGGATATCCCGATTGTGGGGGATGCTCGCCAGGTCCTCGAACAAATGCTTGAACTCTTGTCGCAAGAATCCGCCCATCAACCGCTGGATGAGATCCGCGACTGGTGGCAGCAAATTGAACAGTGGCGCGCTCGTCAGTGCCTGAAATATGACACTCACAGTGAAAAGAtt > 1:31068/1‑250 (MQ=255) ggCAAAGTACTGCCCAAATGCCACTGTTCTGCATATCGATATTGATCCTACTTCCATTTCTAAAACCGTGACTGCGGATATCCCGATTGTGGGGGATGCTCGCCAGGTCCTCGAACAAATGCTTGAACTCTTGTCGCAAGAATCCGCCCATCAACCGCTGGATGAGATCCGCGACTGGTGGCAGCAAATTGAACAGTGGCGCGCTCGGCAGTGCCTGAAATATGACACTCACAGTGAAAAGATTAAACCg < 1:413800/250‑1 (MQ=255) aaaGTACTGCCCAAATGCCACTGTTCTGCATATAGATATTGATCCTACTTCGATTTCTAAAACCGTGACTGCGGATATCCCGATTGTGGGGGATGCTCGCCAGGTCCTCGAACAAATGCTTGAACTCTTGTCGCAAGAATCCGCCCATCAACCGCTGGATGAGATCCGCGACTGGTGGCAGCAAATTGAACAGTGGCGCGCTCGTCAGTGCCTGAAATATGACACTCACAGTGAAAAGATTAAACCGCAgg > 1:158500/1‑251 (MQ=255) cTGCCCAAATGCCACTGTTCTGCATATCGATATTGATCCTACTTCCATTTCTAAAACCGTGACTGCGGATATCCCGATTGTGGGGGATGCTCGCCAGGTCCTCGAACAAATGCTTGAACTCTTGTCGCAAGAATCCGCCCATCAACCGCTGGATGAGATCCGCGACTGGTGGCAGCAAATTGAACAGTGGCGCGCTCGTCAGTGCCTGAAATATGACACTCACAGTGAAAAGATTAAACCGCAGGCGGTgg > 2:173135/1‑250 (MQ=255) caaaaTGCCACTGTTCTGCATATCGATATTGATCCTACTTCCATTTCTAAAACCGTGACTGCGGATATCCCGATTGTGGGGGATGCTCGCCAGGTCCTCGAACAAATGCTTGAACTCTTGTCGCAAGAATCCGCCCATCAACCGCTGGATGAGATCCGCGACTGGTGGCAGCAAATTGAACAGTGGCGCGCTCGTCAGTGCCTGAAATATGACACTCACAGGGAAAAGATTAAACCGCAGGCGGTGATCga < 1:198421/249‑1 (MQ=255) cAAATGCCACTGTTCTGCATATCGATATTGATCCTACTTCCATTTCTAAAACCGTGACTGCGGATATCCCGATTGTGGGGGATGCTCGCCAGGTCCTCGAACAAATGCTTGAACTCTTGTCGCAAGAATCCGCCCATCAACCGCTGGATGAGATCCGCGACTGGTGGCAGCAAATTGAACAGTGGCGCGCTCGTCAGTGCCTGAAATATGACACTCACAGTGAAAAGATTAAACCGCAGGCGGTGATCg > 1:467818/1‑249 (MQ=255) aaaTGCCACTGTTCTGCATATCGATATTGATCCTACTTCCATTTCTAAAACCGTGACTGCGGATATCCCGATTGTGGGGGATGCTCGCCAGGTCCTCGAACAAATGCTTGAACTCTTGTCGCAAGAATCCGCCCATCAACCGCTGGATGAGATCCGCGACTGGTGGCAGCAAATTGAACAGTGGCGCGCTCGTCAGTGCCTGAAATATGACACTCAACGTGAAAAGATTAAACCGCAGGCGGAGATCggg > 1:280581/1‑248 (MQ=255) aTGCCACTGTTCTGCATATCGATATTGATCCTACTTCCATTTCTAAAACCGTGACTGCGGATATCCCGATTGTGGGGGATGCTCGCCAGGTCCTCGAACAAATGCTTGAACTCTTGTCGCAAGAATCCGCCCATCAACCGCTGGATGAGATCCGCGACTGGTGGCAGCAAATTGAACAGTGGCGCGCTCGTCAGTGCCTGAAATATGACACTCACAGTGAAAGGATTAAACCGCAGGCGGGGAACgagccc > 2:94924/1‑248 (MQ=255) cTGCATATCGATATTGATCCTACTTCCATTTCTAAAACCGTTACTGCGCATATCCCGATTGTGCGGGATTCTCGCCAGGTCCTCGACCAAATGCTTGAACTCTTGTCGCAAGAATCAGCCAATCAACCGCTGGATGAGATCCGCGACTGGTGTCAGCACATTGAACAGAGGCGCGCTCGTCAGTGCCTGAAATCTGACACTCACAGTGAAAAGCTTAAACCGCGGGCGGGGACCGAGACACATTGGAGGtt > 2:285250/1‑251 (MQ=255) gCATATCGATATTGATCCTACTTCCATTTCTAAAACCGTGACTGCGGATATCCCGATTGTGGGGGATGCTCGCCAGGTCCTCGAACAAATGCTTGAACTCTTGTCGCAAGAATCCGCCCATCAACCGCTGGATGAGATCCGCGACTGGTGGCAGCAAATTGAACAGTGGCGCGCTCGTCAGTGCCTGAAATATGACACTCACAGTGAAAAGATTAAACCGCAGGCGGTGATCGAGACTCTTTGGCGGtt < 2:204239/249‑1 (MQ=255) gctatTGATCCTTCTTACAGTTCTAAAACCGTTGCTGTGGATAGCCCGATTGTGGGGGATGCTCGCCAGGTCTTCGAACAAATGCTTGAACTCTTTTCGCAAGAATCCGCCCATCAACCGCTGGATGAGATCCGCGACTGGTGGCAGCAAATTGAACAGTGGCGCGCTCGTCAGTGCCTGAAATATGACACTCACAGTGAAAAGATTAAACCGCAGGCGGTGATCGAGACTCTTTGGCGGTTGACGAAggg < 1:428303/249‑1 (MQ=255) tGATCCTACTTCCATTTCTAAAACCGTGACTGCGGATATCCCGATTGTGGGGGATGCTCGCCAGGTCCTCGAACAAATGCTTGAACTCTTGTCGCAAGAATCCGCCCATCAACCGCTGGATGAGATCCGCGACTGGTGGCAGCAACTTGAACAGTGGCGCGCTCGTCAGTGCCTGAAATATGACACTCCCAGTGAAAAGATTAAACCGCAGGCGGAGATCGAGACTCTTTGGCgggtgtggcaggggga > 2:213770/1‑235 (MQ=255) aTCCTACTTCCATTTCTAAAACCGTGACTGCGGATATCCCGATTGTGGGGGATGCTCGCCAGGTCCTCGAACAAATGCTTGAACTCTTGTCGCAAGAATCCGCCCATCAACCGCTGGATGAGATCCGCGACTGGTGGCAGCAAATTGAACAGTGGCGCGCTCGTCAGTGCCTGAAATATGACACTCACAGTGAAAAGATTAAACCGCAGGCGGTGATCGAGACTCTTTGGCGGTTGACGCAGGGAGACGCt > 1:235284/1‑251 (MQ=255) ccTACTTCCATTTCTAAAACCGTGACTGCGGATATCCCGATTGTGGGGGATGCTCGCCAGGTCCTCGAACAAATGCTTGAACTCTTGTCGCAAGAATCCGCCCATCAACCGCTGGATGAGATCCGCGACTGGTGGCAGCAAATTGAACAGTGGCGCGCTCGTCAGTGCCTGAAATATGACACTCACAGTGAAAAGATTAAACCGCAGGCGGTGATCGAGACCCTTTGGCGGTTGACGGAAGGAGACGCttt > 1:122509/1‑250 (MQ=255) cTTCCATTTCTAAAACCGTGACTGCGGATATCCCGATTGTGGGGGATGCTCGCCAGGTCCTCGAACAAATGCTTGAACTCTTGTCGCAAGAATCCGCCCATCAACCGCTGGATGAGATCCGCGACTGGTGGCAGCAAATTGAACAGTGGCGCGCTCGTCAGTGCCTGAAATATTACACTCACAGTGAAAAGATTAAACCGCACGCGGTGATCGAAAATCTTTGGCgggttgggaagagggcagcttgtc > 2:144853/1‑227 (MQ=255) tCCATTTCTAAAACCGTGACTGCGGATATCCCGATTGTGGGGGATGCTCGCCAGGTCCTCGAACAAATGCTTGAACTCTTGTCGCAAGAATCCGCCCATCAACCACTGGATGAGATCCGCGACTGGTGGCAGCAAATTGAACAGTGGCGCGCTCGTCAGTGCCTGAAATATGACACTCACAGTGAAAAGATTAAACCGCAGGCGGTGATCGAGACTCTTTGGCGGTTGACGAAGGGAGACGCTTACGTg < 1:244905/249‑1 (MQ=255) aaCCGTGACTGCGGATATCCCGATTGTGGGGGATGCCCGCCAGGTCCTCGAACAAATGCTTGAACTCTTGTCGCAAGAATCCGCCCATCAACCGCTGGATGAGATCCGCGACTGGTGGCAGCAAATTGAACAGTGGCGCGCTCGTCAGTGCCTGAAATATGACACTCACAGTGAAAAGATTAAACCGCAGGCGGTGATCGAGACTCTTTGGCGGTTGACGAAGGGAGACGCTTACGTGACGTCCGATGTC‑g < 1:83561/251‑1 (MQ=255) ccgtgcgtGCGGATATCCCGATTGGGGGGGATGCTCGACAGGGCCTCGAACAAAGGCTGGAACTCTTGGCTCAAGAATCCGCCCATCAACCGCTGGATGAGATCCGCGACTGGTGGCAGCAAATTGAACAGTGGCGCGCTCGTCAGTGCCGGAAATATGACACTCACAGTGAAAAGATTAAACCGCAGGCGGTGATCGAGACTCTTTGGCGGTTGACGAAGGGAGACGCTTACGTGACGTCCGATGTC‑ggt < 2:280088/244‑2 (MQ=255) gTGACTGCGGATAGCACGATTGTGGGGGATGCTCGCCATGTCCTCGAACAAATGCTTGAACTCTTGTCGCAAGAATCCGCACATCAACCGCTGGATGAGATCCGCGACTGGTGGCAGCAAATTGAACAGTGGCGCGCTCGTCAGTGCCTGAAATATGACACTCACAGTGAAAAGATTAAACCGCAGGCGGTGATCGAGACTCTTTGGCGGTTGACGAAGGGAGACGCTTACGTGACGTCCGATGTC‑GGgca < 2:378430/251‑1 (MQ=255) gttGCGGATATCCCGAGTGTGGGGGATGCACGTCAGGTCCTAGAACAAAGGCTTTAACTCCTGTCACAAGAATCAGCCCAACAACCACGGGATGAGATCCGCGACTGGTGGCAGCAAATTTAACAGGGGGTCGCTCGTCAGTGCCTGAAATATGACACTCACAGTGAAAAGATTAAACCGCAGGCGGTGAGCGAGACTCTTTTGCGGTTGACGAAGGGAGACGCTTACGTGACGGCCGATGTC‑GGgcagca < 2:413124/249‑1 (MQ=255) cGGATAGCCCGATTGATGGGGATGCTGGCCAGGTCCCCGAACAAATGATTGAACTCTGGTCGCAAGAATCCGCCCATCAACCGCTGGATGAGATCCGCGGCTGGGGGCAGCAAATTGAACAGTGGCGCTCTCGTCAGTGCCTGAAATATGACCCTCACAGTGAAAAGATTAAACCGCAGGCGGTGATCGAGACTCTTTGGCGGTTGACGAAGGGAGACGCTTACGGGACGTCGGATGAC‑GGGCAGCACCa < 2:315896/250‑1 (MQ=255) cccGATTGTGGGGGATGCTCGCCAGGTCCTCGAACAAATGCTTGAACTCTTGTCGCAAGAATCCGCCCATCAACCGCTGGATGAGATCCGCGACTGGTGGCAGCAAATTGAACAGTGGCGCGCTCGTCAGTGCCTGAAATATGACACTCACAGTGAAAAGATTAAACCGCAGGCGGTGATCGAGACTCTTTGGCGGTTGACGAAGGGAGACGCTTACGTGACGTCCGATGTC‑GGGCAGCACCAGATGttt < 1:213770/250‑1 (MQ=255) ttGTGGGGGATGCTCGCCAGGTCCTCGAACAAATGCTTGAACTCTTGTCGCAAGAATCCGCCCATCAACCGCTGGATGAGATCCGCGACTGGTGGCAGCAAATTGAACAGTGGCGCGCTCGTCAGTGCCTGAAATATGACACTCACAGTGAAAAGATTAAACCGCAGGCGGTGATCGAGACTCGTTGGCGGTTGACGAAGGGAGACGCGTACGTGACGTCCGATGTC‑GGGCAGCACCAGATGTTtgctgc < 2:306903/250‑1 (MQ=255) aTGCTCGCCAGGTCCTCGAACAAATGCTTGAACTCTTGTCGCAAGAATCCGCCCATCAACCGCTGGATGAGATCCGCGACTGGTGGCAGCAAATTGAACAGTGGCGCGCTCGTCAGTGCCTGAAATATGACACTCACAGTGAAAAGATTAAACCGCAGGCGGTGATCGAGACTCTTTGGCGGTTGACGAAGGGGGGCGCTTACGTGACGGCCGATGGC‑GGGCAGCACCAGATGTTTGCTGGACTTtattat > 1:108960/1‑251 (MQ=255) agggccTCGAACAAATGCTTGAACGCTTGGCGCAAGAATCCGCCCATCAACCGCTGGATGAGATCCGCGACTGGTGGCAGCAAATTGAACAGTGGCGCGCTCGTCAGTGCCTGAAATATGACACTCACATTGAAAAGATTAAACCGCAGGCGGTGATCGAGACTCTTTGGCGGTTGACGAAGGGAGACGCTTACGTGACGTCCGATGTC‑GGTCAGCACCAGATGTTTGCTGCACTTTATTAT‑CCATTCGAc < 1:456146/247‑1 (MQ=255) ccTCGAACAAATGCTTGAACTCTTGTCGCAAGAATCCGCCCATCAACCGCTGGATGAGATCCGCGACTGGTGGCAGCAAATTGAACAGTGGCGCGCTCGTCAGTGCCTGAAATATGACACTCACAGTGAAAAGATTAAACCGCAGGCGGTGATCGAGACTCTTTGGCGGTTGACGAAGGGAGACGCTTACGTGACGTCCGATGTC‑GGGCAGCACCAGATGTTTGCTGCACTTTATTAT‑CCATTCGACAAAc < 2:31068/251‑1 (MQ=255) cAAATGCTTGAACTCTTGTCGCAAGAATCCGCCCATCAACCGCTGGATGAGATCCGCGACTGGTGGCAGCAAATTGAACAGTGGCGCGCTCGTCAGTGCCTGAAATATGACACTCACAGTGAAAAGATTAAACCGCAGGCGGTGATCGAGACTCTTTGGCGGTTGACGAAGGGAGACGCTTACg < 1:289444/184‑1 (MQ=255) tGAACTCTTGTCGCAAGAATCCGCCCATCAACCGCTGGATGAGATCCGCGACTGGTGGCAGCAAATTGAACAGTGGCGCGCTCGTCAGTGCCTGAAATATGACACTCACAGTGAAAAGATTAAACCGCAGGCGGTGATCGAGACTCTTTGGCGGTTGACGAAGGGAGACGCTTACGTGACGTCCGATGTC‑GGGCAGCACCAGATGTTTGCTGCACTTTATTAT‑CCATTCGACAAACCGCGTCGCTGGAt < 1:93251/249‑1 (MQ=255) tGTCGCAAGAATCCGCCCATCAACCGCTGGATGAGATCCGCGACTGGTGGCAGCAAATTGAACAGTGGCGCGCTCGTCAGTGCCTGAAATATGACACTCACAGTGAAAAGATTAAACCGCAGGCGGTGATCGAGACTCTTTGGCGGTTGACGAAGGGAGACGCTTACGTGACGTCCGATGTC‑GGGCAGCACCAGATGTTTGCTGCACTTTATTAT‑CCATTCGACAAACCGCGTCGCTGGATCAATTcc < 1:173135/248‑1 (MQ=255) tGTCGCAAGAATCCGCCCATCAACCGCTGGATGAGATCCGCGACTGGTGGCAGCAAATTGAACAGTGGCGCGCTCGTCAGTGCCTGAAATATGACACTCACAGTGAAAAGATTAAACCGCAGGCGGTGATCGAGACTCTTTGGCGGTTGACGAAGGGAGACGCTTACGTGACGTCCGATGTC‑GGGCAGCACCAGATGTTTGCTGCACTTTATTAT‑CCATTCGACAAACCGCGTCGCTGGATCAATTCCg < 1:180215/249‑1 (MQ=255) gtcagccaGAATCCGCTCATCAACCGCTGGATGAGATCCGCGACTGGTGGCAGCAAATTGAACAGTGGCTCGCTCGTCAGTGCCTGAAATATGACCCTCACAGTGAAAAGATTAAACCGCAGGCGGTGATCGAGACTCTTTGGCGGTTGACGAAGGGATACGCTTACGTGACGTCCGATGTC‑GGGCAGCACCAGATGTTTGCTGCACTTTATTAT‑CCATTCGACAAACCGGGTCGCTGGATCAAGTCCg < 2:78712/242‑1 (MQ=255) gTCGCAAGAATCCGCCCATCAACCGCTGGATGAGATCCGCGACTGGTGGCAGCAAATTGAACAGTGGCGCGCTCGTCAGTGCCTGAAATATGACACTCACAGTGAAAAGATTAAACCGCAGGCGGTGATCGAGACTCTTTGGCGGTTGACGAAGGGGGGCGCTTACGTGACGTCCGATGTC‑GGGCAGCACCAGATGTTTTCTGCACTTTATTTTTCCTTTC‑CCAAACCGCGTCGTCGGGTCAATTCCgggg > 1:365276/1‑249 (MQ=255) caaaaaTCCGCCCACCAACCGCTGGATGAGATCCGCGACTGGTGGCAGCAAATTGAACAGTGGCGCGCTCGTCAGTGCCTGAAATATGACACTCACAGTGAAAAGATTAAACCGCAGGCGGTGATCGAGACTCTTTGGCGGTTGACGAAGGGAGACGCTTACGTGACGTCCGATGTC‑GGGCAGCACCAGATGTTTGCTGCACTTTATTAT‑CCATTCGACAAACCGCGGCGCTGGATCAATTCCGGTGGCCt < 2:297646/247‑1 (MQ=255) aaGAATCCGCCCATCAACCACTGGATGAGATCCGCGACTGGTGGCAGCAAATTGAACAGTGGCGCGCTCGTCAGTGCCTGAAATATGACACTCACAGTGAAAAGATTAAACCGCAGGCGGTGATCGGGACTCTTTGGCGGTTGACGCAGGGAGGAGCTTACGTGGCGGCCCGTGTCCGGGC‑GAACCCGATGGTTTCTTCACTTTATTAT‑ACATTCGTCAAACCGCGGCGCTGGATAAATTTCGGAGGcacc > 2:178217/1‑248 (MQ=255) cAACCGCTGGATGAGATCCGCGACTGGTGGCAGCAAATTGAACAGGGGCGCGCTCGTCAGTGCCTGAAATATGACACTCACAGTGAAAAGATTAAACCGCAGGCGGTGATCGAGACTCTTTGGCGGTTGACGAAGGGAGACGCTTACGTGACGTCCGATGTC‑GGGCAGCACCAGATGTTTGCTGCACTTTATTAT‑CCATTCGACAAACCGCGTCGCTGGATCAATTCCGGTGGCCTCGGCACGATGGGtt < 2:467818/250‑1 (MQ=255) aaaCCGCTGGATGAGATCCGCGACTGGTGGCAGCAAATTGAACAGTGGCGCGCCCGTCAGTGCCTGAAATATGACACTCACAGTGAAAAGAGTAAACCGCAGGCGGTGATCGAGACTCTTTGGCTGTTGACGAAGGGATACGCTTACGTGACGTCCGATGTC‑GGGCTGCACCAGATGTTTGCTGCACTTTATTAT‑CCATGCGACAAACCGCGTCGCTGGATCAATTCCGGTGGCCTCGTCACGATGGGttt < 1:50787/250‑1 (MQ=255) | GAAGCCAATATGACGATGCATAACGCGGATGTGATTTTCGCCGTCGGGGTACGATTTGATGACCGAACGACGAACAATCTGGCAAAGTACTGCCCAAATGCCACTGTTCTGCATATCGATATTGATCCTACTTCCATTTCTAAAACCGTGACTGCGGATATCCCGATTGTGGGGGATGCTCGCCAGGTCCTCGAACAAATGCTTGAACTCTTGTCGCAAGAATCCGCCCATCAACCACTGGATGAGATCCGCGACTGGTGGCAGCAAATTGAACAGTGGCGCGCTCGTCAGTGCCTGAAATATGACACTCACAGTGAAAAGATTAAACCGCAGGCGGTGATCGAGACTCTTTGGCGGTTGACGAAGGGAGACGCTTACGTGACGTCCGATGTC‑GGGCAGCACCAGATGTTTGCTGCACTTTATTAT‑CCATTCGACAAACCGCGTCGCTGGATCAATTCCGGTGGCCTCGGCACGATGGGTTT > NC_000913/86422‑86903 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |