Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,100,070	G→A	C144Y (TGT→TAT)	ycdY →	chaperone protein YcdY

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,100,070	0	G	A	100.0%	165.6 / NA	56	C144Y (TGT→TAT)	ycdY	chaperone protein YcdY
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (23/33);  total (23/33)

CCGATTACAATGCGTTGTTTATCGGCGATGAATGTGCTGTGCCGCCATATCGTAGCGCATGGGTTGAGGGCGCGACGGAAGCGG‑AAGTGCGCGCTTTTCTTTCTGAGCGAGGGATGCCATTAGCGGATACGCCAGCCGATCACATCGGCACAT‑TGCTGCTCGCAGCTTCCTGGCTGGAAGATCAGTCAACGGAAGATGAGAGCGAAGCACTGGAAACACTGTTCAGTGAGTATCTGTTACCCTGGTGTGGTGCGTTCCTTGGCAAAGTGGAGGCCCATGCAACCACGCCTTTCTGGCGCACCATGGCACCGCTAACCCGCGATGCCATTAGTGCAATGTGGGACGAGCTGGAAGAAGATTCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTGTTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCTATCATGCGCGGCATGAACATACTTCTCTC  >  NC_000913/1099824‑1100312                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                   ccGATTACAATGCGTTGTTTATCGGCGATGAATGTGCTGTGCCGCCATATCGTAGAGCCTGGGTTGAGGGCGCGACGGAAGCGG‑AAGTGCGCGCTTTTCTTTCTGAGCGAGGGATGCCATTAGCGGATACGCCAGACGCTCACATCGGCACAT‑TGCTGCTCGCAGCTTCCTGGCTGGAAGATCAGTCAACGGAAGATGAGAGCGAAGCACTGGAAACACTGTTCAGTGAGTATCTGTTACCCtggtatggt                                                                                                                                                                                                                                                >  1:68418/1‑251 (MQ=255)       aCAATGCGTTGTTTATCGGCGATGAATGTGCTGTGCCGCCATATCGTAGCGCATGGGTTGAGGGCGCGACGGAAGCGG‑AAGTGCGCGCTTTTCTTTCTGAGCGAGGGATGCCATTAGCGGATACGCCAGCCGATCACATCGGCACAT‑TGCTGCTCGCAGCTTCCTGGCTGGAAGATCAGTCAACGGAAGATGAGAGCGAAGCACTG‑AAACACTGTTCAGTGAGTATCTGTTACCCTGGTATGGTGCGTTcc                                                                                                                                                                                                                                         >  1:203981/1‑251 (MQ=255)       aCAATGCGTTGTTGATCGGCGATGAATGTGCTGTGCCGCCATATCGTAGCGCATGGGTTGAGGGCGCGACGGAAGCGG‑AAGTGCGCGCTTTTCTTTCTGAGCGAGGGATGCCATTAGCGGATACGCCAGCCGATCACATCGGCACAT‑TGCTGCTCGCAGCTTCCTGGCTGGAAGATCAGTCAACGGAAGATGAGAGCGAAGCACTGGAAACACTGTTCAGTGAGTATCTGTTACCCTGGTATGGTGCGTTc                                                                                                                                                                                                                                          <  1:332510/251‑1 (MQ=255)                     aTCGGCGATGAATGTGCTGTGCCGCCATATCGTAGCGCATGGGTTGAGGGCGCGACGGAAGCGG‑AAGTGCGCGCTTTTCTTTCTGAGCGAGGGATGCCATTAGCGGATACGCCCGCCGATCACATCGGCACAT‑TGCTGCTCGCAGCTTCCTGGCTGGAAGATCAGTCAACGGAAGATGAGAGCGAAGCACTGGAAACACTGTTCAGTGAGTATCTGTTACCCTGGGATGGTGCGTTCCTTGGCAAAGTggg                                                                                                                                                                                                                            >  2:443720/1‑250 (MQ=255)                             aggaggggctgtgcCGCCATGTTGTAGCGCATGGGTTGAGGTCGCGACGGAAGCGG‑AAGTGCGCGCTTTTCTTTCTGAGCGAGGGAGGCCATTAGCGGATACGCCAGCCGATCACATCGGCACAT‑TGCTGCTCGCAGCTTCCTGGCTGGAAGATCAGTCAACGGAAGATGAGAGCGAAGCACTGGAAACACGGTTCAGTGAGTATCTGTTACCCTGGTATGGTGCGTTCCTTGGCAAAGTGGAGGCCCATg                                                                                                                                                                                                                    <  2:247912/244‑1 (MQ=255)                                           cgccATATCGTAGCGCATGGGTTGAGGGCGCGACGGAAGCGG‑AAGTGCGCGCTTTTCTTTCTGAGCGAGGGATGCCATTAGCGGATACGCCAGCCGATCACATCGGCACAT‑TGCTGCTCGCAGCTTCCTGGCTGGAAGATCAGTCAACGGAAGATGAGAGCGAAGCACTGGAAACACTGTTCAGTGAGTATCTGTTACCCTGGTATGGTGCGTTCCTTGGCAAAGTGGAGGCCCATGCAACCACGCCTTTc                                                                                                                                                                                                      >  2:28732/1‑251 (MQ=255)                                             caatatCGTGGCGCATGGGTTGAGGGCGCGA‑GGGAGCGGGAAGGGGGCGCTTTCCTTTCTGAGCGAGGGATGCCTTTAGCGGATCCGCCAGCC‑ATCACATCGGCACAT‑TGCTGCTCGCAGCTTCCTGGCTGGAAGATCAGTCAACGGAAGATGAGAGCGAAGCACTGGAAACACTGTTCAGTGAGTATCTGTTACCCTGGTATGGTGCGTTCCTTGGCAAAGTGGAGGCCCATGCAACCACGCCTTTCTgg                                                                                                                                                                                                   <  1:112477/249‑1 (MQ=255)                                                    gTAGCGCATGGGTTGAGGGCGCGACGGAAGCGG‑AAGTGCGCGCTTTTCTTTCTGAGCGAGGGATGCCATTAGCGGATACGACAGCCGAACACATCGGCACAT‑TGCTGCTCGCAGCTTCCTGGCTGGAAGATCAGTCAACGGAAGATGAGAGCGAAGCACTGGAAACACTGTTCAGTGAGTATCTGTTACCCTGGTATGGTGCGTTCCTTGGCAAAGAGGAGGCCCATGCAACCACGCCTTTCTGGCGCAcc                                                                                                                                                                                             >  1:295373/1‑251 (MQ=255)                                                                  gAGGGCGCGACGGAAGCGG‑AAGTGCGCTCTTTTCTTTCTGAGCGAGGGATGCCATTAGCGGATACGCCCGCCGATCACATCGGCACAT‑TGCTGCTCGCAGCTTCCTGGCTGGAAGATCAGTCAACGGAAGATGAGAGCGAAGCACTGGAAACACTGTTCAGTGAGTATCTGTTACCCTGGTATGGTGCGTTCCTTGGCAAAGTGGAGGCCCATGCAACCACGCCTTTCTGGCGCACCATGGCACCGCTaa                                                                                                                                                                                <  1:463039/250‑1 (MQ=255)                                                                    gggCGCGACGGAAGCGG‑AAGTGCGCGCTTTTCTTTCTGAGCGAGGGATGCCATTAGCGGATACGCCAGCCGATCACATCGGCACAT‑TGCTGCTCGCAGCTTCCTGGCTGGAAGATCAGTCAACGGAAGATGAGAGCGAAGCACTGGAAACACTGTTCAGTGAGTATCTGTTACCCTGGTATGGTGCGTTCCTTGGCAAAGTGGAGGCCCATGCAACCACGCCTTTCTGGCGCACCATGGCACCGCTAAccc                                                                                                                                                                             >  1:284434/1‑251 (MQ=255)                                                                            cggaagcgg‑aagTGCGCGCTTTTCTTTCTGAGCGAGGGATGCCATTAGCGGATACGCCAGCCGATCACATCGGCACAT‑TGCTGCTCGCAGCTTCCTGGCTGGAAGATCAGTCAACGGAAGATGAGAGCGAAGCACTGGAAACACTGTTCAGTGAGTATCTGTTACCCTGGTATGGTGCGTTCCTTGGCAAAGTGGAGGCCCATGCAACCACGCCTTTCTGGCGCACCATGGCACCGCTAACCCGCGATgac                                                                                                                                                                     <  1:223157/251‑3 (MQ=255)                                                                              gaagcgg‑aagTGCGCGCTTTTCTTTCTGAGCGAGGGATGCCATTAGCGGATACGCCAGCCGATCACATCGGCACAT‑TGCTGCTCGCAGCTTCCTGGCTGGAAGATCAGTCAACGGAAGATGAGAGCGAAGCACTGGAAACACTGTTCAGTGAGTATCTGTTACCCTGGTATGGTGCGTTCCTTGGCAAAGTGGATGCCCGTGCAACCACGCCTTTCTGGCGCACCATGGCACCGCTAACCCGAGATGCCAt                                                                                                                                                                   >  2:131800/1‑251 (MQ=255)                                                                                             cgcTTTTCTTTCTGAGCGAGGGATGCCATTAGCGGATACGCCAGCCGATCACATCGGCACAT‑TGCTGCTCGCAGCTTCCTGGCTGGAAGATCAGTCAACGGAAGATGAGAGCGAAGCACTGGAAACACTGTTCAGTGAGTATCTGTTACCCTGGTATGGTGCGTTCCTTGGCAAAGTGGAGGCCCATGCAACCACGCCTTTCTGGCGCACCATGGCACCGCTAACCCGCGATGCCATTAGTGCAATGTggg                                                                                                                                                     <  1:121098/251‑1 (MQ=255)                                                                                             cgcTTTTCTTTCTGAGCGAGGGATGCCATTAGCGGATACGCCAGCCGATCACATCGGCACAT‑TGCTGCTCGCAGCTTCCTGGCTGGAAGATCAGTCAACGGAAGATGAGAGCGAAGCACTGGAAACACTGTTCAGTGAGTATCTGTTACCCTGGTATGGTGCGTTCCTTGGCAAAGTGGAGGCCCATGCAACCACGCCTTTCTGGCGCACCATGGCACCGCTAACCCGCGATGCCATTAGTGCAATGTggg                                                                                                                                                     >  1:91414/1‑251 (MQ=255)                                                                                              gcTTTTCTTTCTGAGCGAGGGATGCCATTAGCGGATACGCCAGCCGATCACATCGGCACAT‑TGCTGCTCGCAGCTTCCTGGCTGGAAGATCAGTCAACGGAAGATGAGAGCGAAGCACTGGAAACACTGTTCAGTGAGTATCTGTTACCCTGGTATGGTGCGTTCCTTGGCAAAGTGGAGGCCCATGCAACCACGCCTTTCTGGCGCACCATGGCACCGCTAACCCGCGATGCCATTAGTGCAATGTggta                                                                                                                                                    <  1:103356/251‑3 (MQ=255)                                                                                               ttttgttttttgagcggggagtGCCATTAGCGGTTACGCCAGCCGATCACATCGGAACAT‑TGCTGCTCGCAGCTTACGGGCTGGAAGATCAGTCAACGGAAGATGAGAGCGAAGCACTGGAAACACTGTGCAGTGAGTATCTGTTACCCTGGTATGGTGCGTTCCTTGGCAAAGTGGAGGCCCATGCAACCACGCCTTTCTGGCGCACCATGGCACCGCTAACCCGCGAGGCCATTAGTGCAATGTGGGa                                                                                                                                                    <  2:451735/229‑1 (MQ=255)                                                                                                ttttCTTTCTGAGCGAGGGATGCCATTAGCGGATACGCCAGCCGATCACATCGGCACAT‑TGCTGCTCGCAGCTTCCTGGCTGGAAGATCAGTCAACGGAAGATGAGAGCGAAGCACTGGAAACACTGTTCAGTGAGTATCTGTTACCCtggtatggt                                                                                                                                                                                                                                                <  1:348265/157‑1 (MQ=255)                                                                                                ttttCTTTCTGAGCGAGGGATGCCATTAGCGGATACGCCAGCCGATCACATCGGCACAT‑TGCTGCTCGCAGCTTCCTGGCTGGAAGATCAGTCAACGGAAGATGAGAGCGAAGCACTGGAAACACTGTTCAGTGAGTATCTGTTACCCtggtatggt                                                                                                                                                                                                                                                >  2:348265/1‑157 (MQ=255)                                                                                                ttttCTTTCTGAGCGAGGGATGCCATTAGCGGATACGCCAGCCGATCACATCGGCACAT‑TGCTGCTCGCAGCTTCCTGGCTGGAAGATCAGTCAACGGAAGATGAGAGCGAAGCACTGGAAACACTGTTCAGTGAGTATCTGTTACCCTGGTATGGTGCGTTCCTTGGCAAAGTGGAGGCCCATGCAACCACGCCTTTCTGGCGCACCATGGCACCGCTAACCAGCGATGCCATTAGTGCAATGTGGGACg                                                                                                                                                  >  1:290347/1‑251 (MQ=255)                                                                                                 tttctttcTGAGCGAGGGATGCCATTAGCGGATACGCCAGCCGATCACATCGGCACAT‑TGCTGCTCGCAGCTTCCTGGCTGGAAGATCAGTCAACGGAAGATGAGAGCGAAGCACTGGAAACACTGTTCAGTGAGTATCTGTTACCCTGGTATGGTGCGTTCCTTGGCAAAGTGGAGGCCCATGCAACCACGCCTTTCTGGCGCACCATGGCACCGCTAACCCGCGATGCCATTAGTGCAATGTGGGACg                                                                                                                                                  <  1:387095/250‑1 (MQ=255)                                                                                                      ttctGAGCGAGGGATGCCATTAGCGGATACGCCAGCCGATCACATCGGCACAT‑TGCTGCTCGCAGCTTCCTGGCTGGAAGATCAGTCAACGGAAGATGAGAGCGAAGCACTGGAAACACTGTTCAGTGAGTATCTGTTACCCTGGTATGGTGCGTTCCTTGGCAAAGTGGAGGCCCATGCAACCACGCCTTTCTGGCGCACCATGGCACCGCTAACCCGCGATGCCATTAGTGCAATGTGGGACGAGCTg                                                                                                                                             <  1:457682/250‑1 (MQ=255)                                                                                                          gAGCGAGGGATGCCATTAGCGGATACGCCAGCCGATCACATCGGCACAT‑TGCTGCTCGCAGCTTCCTGGCTGGAAGATCAGTCAACGGAAGATGAGAGCGAAGCACTGGAAACACTGTTCAGTGAGTATCTGTTACCCTGGTATGGTGCGTTCCTTGGCAAAGTGGAGGCCCATGCAACCACGCCTTTCTGGCGCACCATGGCACCGCTAACCCGCGATGCCATTAGTGCAATGTGGGACGAGCTGgaaga                                                                                                                                        <  2:284434/251‑1 (MQ=255)                                                                                                           ggCGAGGGATGCCCTTAGCGGATACGCCAGCCGATCACATCGGCACAT‑TGCTGCTCGCAGCTTCCTGGCTGGAAGATCAGTCAACGGAAGATGAGAGCGAAGCACTGGAAACACTGTTCAGTGAGTATCTGTTACCCTGGTATGGTGCGTTCCTTGGCAAAGTGGAGGCCCATGCAACCACGCCTTTCTGGCGCACCATGGCACCGCTAACCCGCGATGCCATTAGTGCAATGTGGGACGAGCTGgaagaa                                                                                                                                       <  1:426592/250‑1 (MQ=255)                                                                                                              gtgggtgtccATTAGGGGATACGATAGTCGATCACATCGG‑ACAAATGCTGCTCGCAGCTTCCTGGCTGGAAGATCAGTCAACGGAAGATGAGAGCGAAGCCCTGGAAACACTGTTTAGTGAGTATCTGTTACCCTGGTATGGTGCGTTCCTTGGCAAAGTGGAGTCCAATGCAACCACGCCTTTCGGGCGCACCATGGCAACGCTAACCCGCGATGCCATTAGTGCAATGTGGGACGAGCTGGAAGAAGAt                                                                                                                                    <  2:375734/243‑1 (MQ=255)                                                                                                              gAGGGATGCCTTGAGCTGATACTCCAGCCGATCACAGCGGCAAAT‑TGCTGCTCGCAGCTGCCTGGCTGGAAGATCAGTCAACGGAAGATGAGAGCGAAGCACTGGAAACACTGTTCAGTGAGTATCTGTTACCCTGGTATGGTGCGTTCCTTGGCAAAGTGGAGGCCCATGCAACCACGCCTTGCTGGCGCACCATGGCACCGCTAACCCGCGATGCCATTAGTGCAATTTGGGACTAGCGTGAAGAAGAt                                                                                                                                    <  2:430879/251‑1 (MQ=255)                                                                                                               aGGGATGCCATTAGCGGATACGCCAGCCGATCACATCGGCACAT‑TGCTGCTCGCAGCTTCCTGGCTGGAAGATCAGTCAACGGAAGATGAGAGCGAAGCACTGGAAACACTGTTCAGTGAGTATCTGTTACCCTGGTATGGTGCGTTCCTTGGCAAAGTGGAGGCCCATGCAACCACGCCTTTCTGGCGCACCATGGCACCGCTAACCCGCGATGCCATTAGTGCAATGTGGGACGAGCTGGAAGAAGAtt                                                                                                                                   <  1:28732/251‑1 (MQ=255)                                                                                                                  gATGCCATTAGCGGATACGACAGCAGTTCACATCGGAACAT‑TGATGATCGCAGCTTCCTGGCTGGCAGATCAGTCACCGGAAGCTGAGAGCGAAGCACTGGAAACACTGTTCAGTGAGAATCTGTTACCCAGGTATGGTACGTTCCTTGGCCCAGTGGATGCCCATGCAAACACGCCTTTCTGGCGCACCATGGCCCCGCTAACACGCGCTGCCATTAGTACAATGTGGGACGAGACGGAAGAAGATTCTg                                                                                                                                >  2:418153/1‑251 (MQ=255)                                                                                                                     gCCATTAGCGGATACGCCAGCCGATCACATCGGCACAT‑GGCTGCTCGCAGCTTCCTGGCTGGAAGATCAGTCAACGGAAGATGAGAGCGAAGCACGGGAAACACTGTTCAGTGAGTATCTGTTACCCTGGTATGGTGCGTTCCTTGGCAAAGTGGAGGCCCATGCAGCCACGCCTTTCTGGCGCACCATGGCACCGCTAACCCGCGATGCCATTAGTGCAATGTGGGACGAGCTGGAAGAAGATTCTgaa                                                                                                                              <  1:275324/250‑1 (MQ=255)                                                                                                                                   cGCCAGCCGATCACATCGGCACAT‑TGCTGCTCGCAGCTTCCTGGCTGGAAGATCAGTCAACGGAAGATGAGAGCGAAGCACTGGAAACACTGTTCAGTGCGTATCTGTTACCCTGGTATGGTGCGTTCCTTGGCAAAGTGGAGGCCCATGCAACCACGCCTTTCTGGCGCACCATGGCACCGCTAACCCGCGATGCCATTAGTGCAATGTGGGACGAGCTGGAAGAAGATTCTGCAGAATAAGTGTGATCt                                                                                                               >  1:464391/1‑251 (MQ=255)                                                                                                                                      cAGCCGATCACATCGGCACAT‑TGCTGCTCGCAGCTTCCTGGCTGGAAGATCAGTCAACGGAAGATGAGAGAGAAGCACTGGAAACACTGTTCAGTGAGTATCTGTTACCCTGGGATGGTGCGTTCCTTGGCAAAGTGGAGGCCCATGCAACCAAGCCTTTCTGGCGCACCATGGCACAGCTAACCCGCGATGCCATTAGTGCAATGTGGGACGAGCTGAAAGCAGATTCTGAAGAATAAGTGTGATCTACg                                                                                                            >  1:310426/1‑251 (MQ=255)                                                                                                                                         ccGATCACATCGGCACAT‑TGCTGCTCGCAGCTTCCTGGCTGGAAGATCAGTCAACGGAAGATGAGAGCGAAGCACTGGAAACACTGTTCAGTGAGTATCTGTTACCCTGGTATGGTGCGTTCCTTGGCAAAGTGGAGGCCCATGCAACCACGCCTTTCTGGCGCACCATGGCACCGCTAACCCGCGATGCCATTAGTGCAATGTGGGACGAGCTGGAAGAAGATTCTGAAGAATAAGTGTGATCTACGTCa                                                                                                         <  1:430474/251‑1 (MQ=255)                                                                                                                                         ccGATCACAGCGGCACAT‑TGCTGCTCGCAGCTTCCTGGCTGGAAGAACAGTCAACGGAAGATGAGAGCGAAGCACTGGAAACACTGTTCAGTGAGTATCTGTTACCATGGTATGGTGCGTTCCTTGGCAAAGTGGAGGCCCATGCAACCACGCCTTTCTGGCGCACCATGGCACCGCTAACCCGCGATGCCATTAGTGCAATGTGGGACGAGCTGGAAGAAGATTCTGAAGAATAAGTGTGATCTACGta                                                                                                          >  2:375496/1‑249 (MQ=255)                                                                                                                                                   cGGCACAT‑TGCTGCTCGCAGCTTCCTGGCTGGAAGATCAGTCAACGGAAGATGAGAGCGAAGCACTGGAAACACTGTTCAGTGAGTATCTGTTACCCTGGTATGGTGCGTTCCTTGGCAAAGTGGAGGCCCATGCAACCACGCCTTTCTGGCGCACCATGGCACCGCTAACCCGCGATGCCATTAGTGCAATGTGGGACGAGCTGGAAGAAGATTCTGAAGAATAAGTGTGATCTACGTCACTCATAACTg                                                                                               >  1:31038/1‑251 (MQ=255)                                                                                                                                                      cacaT‑TGCTGCTCGCAGCTTCCTGGCTGGAAGATCAGTCAACGGAAGATGAGAGCGAAGCACTGGAAACACTGTTCAGTGAGTATCTGTTACCCTTGTATGGTGCGTTCCTTGGCAAAGTGGAGGCCCATGCAACCACGCCTTTCTGGCGCACCATGGCACCGCTAACCCGCGATGCCATTAGTGCAATGTGGGACGAGCTGGAAGAAGATTCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCaa                                                                                            <  1:362911/251‑1 (MQ=255)                                                                                                                                                                   cGCAGCTTCCTGGCTGGAAGATCAGTCAACGGAAGATGAGAGCGAAGCACTGGAAACACTGTTCAGTGAGTATCTGTTCCCCTGGTATGGTGCGTTCCTTGGCAAAGTGGAGGCCCATGCAACCCCGCCTTTCTGGCGCACCATGGCACCGCTAACCCGCGATGCCATTCGTGCAATGTGGGACGAGCTGGAAGAAGATTCTGAAGAATAAGTGTGAt                                                                                                                 >  1:445495/1‑218 (MQ=255)                                                                                                                                                                          tCCTGGCTGGAAGATCAGTCAACGGAAGATGAGAGCGAAGCACTGGAAACACTGTTCAGTGAGTATCTGTTACCCTGGTATGGTGCGTTCCTTGGCAAAGTGGAGGCCCATGCAACCACGCCTTTCTGGCGCACCATGGCACCGCTAACCCGCGATGCCATTAGTGCAATGTGGGACGAGCTGGAAGAAGATTCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATttgttgttg                                                                           <  1:131800/249‑1 (MQ=255)                                                                                                                                                                              ggctggAAGATCAGTCAACTGAAGATGTGAGCGAAGCACGGGAACCACTGTTCAGGGAGTATCTTTTACCCTGGTATGGTGCGTTCCTTGGCAAAGTGGAGGCCCATGCAACCACGCCTTTCTGGCGCACCATGGCACCGCTAACCCGCGATGCCATTAGTGCAATGTGGGACGAGCTGGAAGAAGATTCTGAAGAATAAGTGTGATATACGTCTCGCATAACTGCAACGGATAATTTGTTGTTGCATaaa                                                                     <  2:326385/251‑1 (MQ=255)                                                                                                                                                                                gtggAAGATCAGGTAACTGAAGATGAGAGCGAAGCACTGGAAACACTGTGCAGTGAGTATCTGTTATCCTGGTATGGTGCGTTCCTTGGCAAAGTGGAGGCCCATGCAACCACGCCTTTCTGGCGCACCATGGCACCGCTAACCCGCGATGCCATTAGTGCAATGTGGGACGAGCTGGAAGAAGATGCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAAAGGATAAGTTGTTGTTGCATAAAAt                                                                   <  2:338797/250‑1 (MQ=255)                                                                                                                                                                                    aaGATCAGTAAACGGAAGATGAGAGCGAATCACTGGAAACACTGTTCAGTGAGTATCTGTTACCCTGGTATGGTGCGTTCCTTGGCAAAGTGGAGGCCCATGCAACCACGCCTTTCTGGCGCACCATGGCACCGCTAACCCGCGATGCCATTAGAGCAATGTGGGACGAGCTGGAAGAAGATTCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTGTTGTTGCATAAAAtgtgt                                                               >  1:356010/1‑251 (MQ=255)                                                                                                                                                                                        tCAGTCAACGGAAGATGAGAGCGAAGCACTGGAAACACTGTTCAGTGAGTATCTGTTACCCTGGTATGGTGCGTTCCTTGGCAATGTGGAGGCCCATGCAACCACGCCTTTCTGGCGCACCATGGCACCGCTAACCCGCGATGCCATTAGTGCAATGTGGGACGAGCTGGAAGAAGATTCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCACCGGATAATTTGTTGTTGCATAAAATGTGTGCTc                                                           >  2:42194/1‑251 (MQ=255)                                                                                                                                                                                        tCAGTCAACGGAAGATGAGAGCGAAGCACTGGAAACACTGTTCAGTGAGTATCTGTTACCCTGGTATGGTGCGTTCCTTGGCAACGTGGAGGCCCATGCAACCACGCCTTTCTGGCGCACCATGGCACCGCTAACCCGCGATGCCATTAGTGCAATGTGGGACGAGCTGGAAGAAGATTCTGAAGAATAAGGGTGATCTACGTAGCTCATAACTGCACCGGATAATTTGTTGTTGCATAAAATGTGTGCTc                                                           >  2:239353/1‑251 (MQ=255)                                                                                                                                                                                         cAGTCAACGGAAGATGAGAGCGAAGCACTGGAAACACTGTTCAGTGAGTATCTGTTACCCTGGTATGGTGCGTTCCTTGGCAAAGTGGAGGCCCATGCAACCACGCCTTTCTGGCGCACCATGGCACCGCTAACCCGCGATGCCATTAGTGCAATGTGGGACGAGCTGGAATAAGATTCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTGTTGTTGCATAAAATGTGTGCTCg                                                          <  2:429110/251‑1 (MQ=255)                                                                                                                                                                                         cAGTCAACGGAAGATGAGAGCGAAGCACTGGAAACACTGTTCAGTGAGTATCTGTTACCCTGGTATGGTGCGTTCCTTGGCAAAGTGGAGGCCCATGCAACCACGCCTTTCTGGCGCACCATGGCACCGCTAACCCGCGATGCCATTAGTGCAATGTGGGACGAGCTGGAAGAAGATTCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTGTTGTTGCATAAAATGTGTGCTCg                                                          <  1:470616/251‑1 (MQ=255)                                                                                                                                                                                             cAACGGAAGATGAGAGCGAAGCACTGGAAACACTGTTCAGTGATTATCTGTTACCCTGGTATGGTGCGTTCCTTGGCAAAGTGGAGGCCCATGCAACCACGCCTTTCTGGCGCACCATGGCACCGCTAACCCGCGATGCCATTAGTGCAATGTGGGACGAGCTGGAAGAAGATTCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTGTTGTTGCATAAAATGTGTGCTCGAtct                                                      <  2:229440/251‑1 (MQ=255)                                                                                                                                                                                                cGGAAGATGAGAGCGAAGCACTGGAAACACTGTTCAGTGAGTATCTGTTACCCTGGTATGGTGCGTTCCTTGGCAAAGTGGAGGCCCATGCAACCACGCCTTTCTGGCGCACCATGGCACCGCTAACCCGCGATGCCATTAGTGCAATGTGGGACGAGCTGGAAGAAGATTCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTGTTGTTGCATAAAATGTGTGCTCGATCtcat                                                   <  1:326318/251‑1 (MQ=255)                                                                                                                                                                                                 ggAAGATGAGGGCGAAGTACGTGAAACGCTGGTCAGTGAGTGTCTGGTACCCTGGTATTGTGCGTTCCTTGGCAAAGTGGAGGCCCATGCAACCACGCCTTTCTGGCGCACCATGGCACCGCTAACCCGAGATGCCATTAGTGCAATGTGGGACGAGCTGGAAGAAGATTCTGAAGAATATGTGTGATCTACGTTACTCATAACTGCAACGGATAATTTGTTGTTGCATAAAATGTGTGCGCGATCtcatt                                                  <  2:259371/251‑1 (MQ=255)                                                                                                                                                                                                      aTGAGAGCGAAGCACTGGAAACACTGTTCAGTGAGTATCTGTTACCCTGGTATGGTGCGTTCCTTGGCAAAGTGGAGGCCCATGCAACCACGCCTTTCTGGCGCACCATGGCACCGCTAACCCGCGATGCCATTAGTGCAATGTGGGACGAGCTGGAAGAAGATTCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTGTTGTTGCATAAAATGTGTGCTCGAtc                                                       >  1:468947/1‑241 (MQ=255)                                                                                                                                                                                                      aTGAGAGCGAAGCACTGGAAACACTGTTCAGTGAGTATCTGTTACCCTGGTATGGTGCGTTCCTTGGCAAAGTGGAGGCCCATGCAACCACGCCTTTCTGGCGCACCATGGCACCGCTAACCCGCGATGCCATTAGTGCAATGTGGGACGAGCTGGAAGAAGATTCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTGTTGTTGCATAAAATGTGTGCTCGAtc                                                       <  2:468947/241‑1 (MQ=255)                                                                                                                                                                                                         agagCGAAGCACTGGAAACACTGTTCAGTGAGTATCTGTTACCCTGGTATGGTGCGTTCCTTGGCAAAGTGGAGGCCCATGCAACCACGCCTTTCTGGCGCACCATGGCACCGCTAACCCGCGATGCCATTAGTGCAATGTGGGACGAGCTGGAAGAAGATTCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTGTTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCcg                                          <  1:141460/251‑1 (MQ=255)                                                                                                                                                                                                                          actcTGTTCAGGGCGTATCTTTTACCCTGGTATGGTGCGTTCCTTGGCAAATTGGAGGCCCTTGCAACCACGCCTTTCTGGCGCACCATGGCACCGCTAACCCGCGATGCCATTAGTGCAATGTGGGACGAGCTGGAAGAAGATTCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTGTTTTTTCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCTATCATg                         <  2:447477/248‑1 (MQ=255)                                                                                                                                                                                                                            ccTGTTCAGTGAGTATCTGTTACCCTGGGATGGTGCGTTCCGTGGCAAAGTGGAGGCACATGCAACCACGCCTTTCTGGCGCACCATGGCACCGCTAACCCGCGATGCCATTAGTGCAATGTGGGACGAGCTGGAAGAAGATTCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTGTTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCTATCATgcg                       <  2:98123/250‑1 (MQ=255)                                                                                                                                                                                                                               gtacAGTGAGTATCTGTTTCCCTGGTATGGTGCGTTCCTTGGCAAAGTGGAGGCCCATGCAACCACGCCTTTCTGGCGCACCATGGCACCGCTAACCCGCGATGCCATTAGTGCAATGTGGGACGAGCTGGAAGAAGATTCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTGTTGTTGCATAAAATGTGGGCTCGATCTCATTCATGGCCGCGTTTTCTGCTATCATGCGCgg                    <  2:31038/248‑1 (MQ=255)                                                                                                                                                                                                                                 tCAGTGAGGAACTGTTACCCTGGTATGGTGCGTTCGTTGGCAAAGTGTAGGCCCATGCAACCACGCCTTTCTGGCGCAACTTGGCACCGCTAACTCTCGATGTCATTAGTTCAATGTGGGACGAGCTGGAAGAAGAGTCTGAAGAATAAGTGTGAGTTACGTCACTCATATCTGCAACGGATAATTTGTTGTTGCATAAAATGTGTGCGCGATCTCATTCATGGCCGCGTTTTCTGCTATCATGCGCggt                   <  2:187736/250‑2 (MQ=255)                                                                                                                                                                                                                                  cAGTGAGTATCTGTTACCCTGGTATGGTGCGTTCCTTGGCAAAGTGGAGGCCCATGCAACCACGCCTTTCTGGCGCACCATGGCACCGCTAACCCGCGATGCCATTAGTGCAATGTGGGACGAGCTGGAAGAAGATTCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTGTTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCTATCATGCGCGGCAt                 >  1:435074/1‑251 (MQ=255)                                                                                                                                                                                                                                   aGTGAGTATCTGTTACCCTGGTATGGTGCGTTCCTTGGCAAAGTGGAGGCCCATGCAACCACGCCTTTCTGGCGCACCATGGCACCGCTAACCCGCGATGCCATTAGTGCAATGTGGGACGAGCTGGAAGAAGATTCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTGTTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCTATCATGCGCGGCATg                >  1:36582/1‑251 (MQ=255)                                                                                                                                                                                                                                   aGTGAGTATCTGTTACCCTGGTATGGTGCGTTCCTTGGCAAAGTGGAGGCCCATGCAACCACGCCTTTCTGGCGCACCATGGCACCGCTAACCCGCGATGCCATTAGTGCAATGTGGGACGAGCTGGAAGAAGATTCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTGTTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCTATCATGCGCGGCATg                >  1:381512/1‑251 (MQ=255)                                                                                                                                                                                                                                      gaggagcTGTTACCCTGGTATGGTGCGTTCCTTGGCAAAGTGGAGGCCCATGCAACCACGCCTTTCTGGCGCACCATGGCACCGCTAACCCGCGATGCCATTAGTGCAATGTGGGACGAGCTGGAAGAAGATTCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTGTTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCTATCATGCGCGGCATGAAc             <  1:103041/245‑1 (MQ=255)                                                                                                                                                                                                                                       aGTATCTGTTACCCTGGTATGGTGCGTTCCTTGGCAAAGTGGAGGCCCATGCAACCACGCCTTTCTGGCGCACCATGGCACCGCTAACCCGCGATGCCATTAGTGCAATGTGGGACGAGCTGGAAGAAGATTCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTGTTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCTATCATGCGCGGCATgcac             >  2:180541/1‑247 (MQ=255)                                                                                                                                                                                                                                                 acacTGGTATGGTGCGTTCCTTGGCAAAGTGGAGGCCCATGCAACCACGCCTTTCTGGCGCACCATGGCACCGCTAACCCGCGATGCCATTAGTGCAATGTGGGACGAGCTGGAAGAAGATTCTGAAGAATAAGTGTGATCTACGTCACTCATACCTGCAACGGATAATTTGTTGTTGCATAAAATGTGTGCTAGATCTCATTAATGGCCGCGTTTTCTGCTATCATGCGCGGCCTGACCATACTtctatc  >  2:351547/4‑248 (MQ=255)                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                   CCGATTACAATGCGTTGTTTATCGGCGATGAATGTGCTGTGCCGCCATATCGTAGCGCATGGGTTGAGGGCGCGACGGAAGCGG‑AAGTGCGCGCTTTTCTTTCTGAGCGAGGGATGCCATTAGCGGATACGCCAGCCGATCACATCGGCACAT‑TGCTGCTCGCAGCTTCCTGGCTGGAAGATCAGTCAACGGAAGATGAGAGCGAAGCACTGGAAACACTGTTCAGTGAGTATCTGTTACCCTGGTGTGGTGCGTTCCTTGGCAAAGTGGAGGCCCATGCAACCACGCCTTTCTGGCGCACCATGGCACCGCTAACCCGCGATGCCATTAGTGCAATGTGGGACGAGCTGGAAGAAGATTCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTGTTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCTATCATGCGCGGCATGAACATACTTCTCTC  >  NC_000913/1099824‑1100312

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑