Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	89,241	C→A	intergenic (+209/‑393)	cra → / → mraZ	DNA‑binding transcriptional dual regulator Cra/DNA‑binding transcriptional repressor MraZ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	89,241	0	C	A	92.3%	100.8 / ‑2.6	39	intergenic (+209/‑393)	cra/mraZ	DNA‑binding transcriptional dual regulator Cra/DNA‑binding transcriptional repressor MraZ
Reads supporting (aligned to +/- strand):  ref base C (2/1);  new base A (13/23);  total (15/24)
Fisher's exact test for biased strand distribution p-value = 5.47e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

AATCTCTATCGCCGCGGCGTGCTCAGCCGTAGCTAAGCCGCGAACAAAAATACGCGCCAGGTGAATTTCCCTCTGGCGCGTAGAGTACGGGACTGGACATCAATATGCTTAAAGTAAATAAGACTATTCCTGACTATTATTGATAAATGCTTTTAAACCCGCCCGTTAATTAACTCACCAGCTGAAATTCACAATAATTAAGTGATATCGACAGCGCGTTTTTGCATTATTTTGTTACATGCGGCGATGAATTGCCGATTTAACAAACACTTTTCTTTGCTTTTGCGCAAACCCGCTGGCATCAAGCGCCACACAGACGTAACAAGGACTGTTAACCGGGGAAGATATGTCCTAAAATGCCGCTCGCGTCGCAAACTGACACTTTATATTTGCTGTGGAAAATAGTGAGTCATTTTAAAACGGTGATGACGATGAGGGATTTTTTCTTACAGCTATTCATAACGTTAATTTGCTTCGCACGTT  >  NC_000913/88997‑89479                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                               aaTCTCTATCGCCGCGGCGTGCTCAGCCGTAGCTAAGACGCGAAAAAAAATACGCGCCAGGTGAATTTCCCTCTGGCGCGTAGAGTACGGGAATGGACATCAATATCCTTAAAGTAAATAAGACTATTCCTGACTATTATTGATAAGTTCTTTTAAACCCGCCCGTTAATTAACTCACCAGCTGAAATTCACAATAATTAAGTGATATCGACAGCGCGTTTTTGCATTATTTTGTTACATGCAGAGATGa                                                                                                                                                                                                                                           <  2:430056/250‑1 (MQ=255)   ccgctATCGCCGCGGCGTGTTCAGCCGTAGCTAAGCCGCGAACAAAAATACGCGCCAGGTGAATTTCCCTCTGGCGCGTAGAGTACGGGAATGGACATCAATATCCTTAAAGTAAATAAGACTATTCCTGACTATTATTGATAAGTTCTTTTAATTCCGCCCGTTAATTAACTCACCAGCTGAAATTCACAATAATTAAGTGATATCGACAGCGCGTTTTTGCCTTATTTTGTTACATGCAGAGATGAAtt                                                                                                                                                                                                                                        <  2:238568/248‑1 (MQ=255)        aTCGCCGCGGCGTGCTTAGCCGTATCTAAGCCGTGAACAAAAATACGCGTCAGGTTAATGTTCCTCTGGCGCGTAGAGTACGTGAATGGACTGCAATATCCGTAAATTAAATAAGACTATTCCTGACTATTATTGAGAAGTTCTATTAAACCCGCCCGTTAAGTAACTCATCAGCTGAAATTCACAATAATTAATGGATATCGACAGCGCGTTTTTGCATTATTTTGTTACATGCAGAGATGAATTGcc                                                                                                                                                                                                                                     <  2:336737/249‑1 (MQ=255)           gccgcGGCGTGCTCAGCCGTAGCTAAGCCGCGCACAAAAATACGCGCCAGGTGAATTTCCCTCTGGCGCGTAGAGTACGGGAATGGACATCAATATCCTTAAAGTAAATAAGACTATTCCTGACTATTATTGATAAGTTCTTTTAAACCCGCCCGTTAATTAACTCACCAGCTGAAATTCACAATAATTAAGTGATATCGACAGCGCGTTTTTGCCTTATTTTGTTACATGCAGAGATGAATTGCCGAttt                                                                                                                                                                                                                                >  1:282891/1‑251 (MQ=255)                        cAGCCGTAGCTAAGCCGCGAACAAAAATACGCGCCAGGTGAATTTCCCTCTGGCGCGTAGAGTACGGGAATGGACATCAATATCCTTAAAGTAAATAAGACTATTCCTGACTATTATTGATAAGTTCTTTTAAACCCGCCCGTTAATTAACTCACCAGCTGAAATTCACAATAATTAAGTGATATCGACAGCGCGTTTTTGCATTATTTTGTTACATGCAGAGATGAATTGCCGATTTAACAAGCACttt                                                                                                                                                                                                                    <  1:309885/250‑1 (MQ=255)                             gTAGCTAAGCCGCGAACAAAAATACGCGCCAGGTGAATTTCCCTCTGGCGCGTAGAGTACGGGAATGGACATCAATATCCTTAAAGTAAATAAGACTATTCCTGACTATTATTGATAAGTTCTTTTAAACCCGCCCGTTAATTAACTCACCAGCTGAAATTCACAATAATTAAGTGATATCGACAGCGCGTCTTTGCATTATTTTGTTACATGCAGAGATGAATTGCCGATTTAACAAGCACTTTTCTTTg                                                                                                                                                                                                              <  1:363889/251‑1 (MQ=255)                               aGCTAAGCCGCGAACAAAAATACGCGCCAGGTGAATTTCCCTCTGGCGCGTAGAGTACGGGACTGGACATCAATATGCTTAAAGTAAATAAGACTATTCCTGACTATTATTGATAAATGCTTTTAAACCCGCCCGTTAATTAACTCACCAGCTGAAATTCACAATAATTAAGTGATATCGACAGCGCGTTTTTGCATTATTTTGTTACATGCGGCGATGAATTGTCGATTTAACAAACACTTTTCTTTGCt                                                                                                                                                                                                            <  2:3501/251‑1 (MQ=255)                                gCTAAGCCGCGAACAAAAATACGCGCCAGGTGAATTTCCCTCTGGCGCGTAGAGTACGGGAATGGACATCAATATCCTTAAAGTAAATAAGACTATTCCTGACTATTATTGATAAGTTCTTTTAAACCCGCCCGTTAATTAACTCACCAGCTGAAATTCACAATAATTAAGTGATATCGACAGCGCGTTTTTGCATTATTTTGTTACATGCAGAGATGAATTGCCGATTTAACAAGCACTTTTCTTTGCtt                                                                                                                                                                                                           >  2:159098/1‑251 (MQ=255)                                 cTAAGCCGCGCACACAAATACGCGCCAGGTGAATTTCCCTCTGGCGCGTAGAGTACGGGAATGGACATCAATATCCTTAAAGTAAATAAGACTATTCCTGACTATTATTGATAAGTTCTTTTAAACCCGCCCGTTAATTAACTCACCAGCTGAAATTCACAATAATTAAGTGATATCGACAGCGCGTTTTTGCATTATTTTGTTACATGCAGAGATGAATTGCCGATTTAACAAGAACTTTTCTTTGCttt                                                                                                                                                                                                          >  2:36265/1‑251 (MQ=255)                                            aCAAAAATACGCGCCAGGTGAATTTCCCTCTGGCGCGTAGAGTACGGGAATGGACATCAATATCCTTAAAGTAAATAAGACTATTCCTGACTATTATTGATAAGTTCTTTTAAACCCGCCCGTTAATTAACTCACCAGCTGAAATTCACAATAATTAAGTGATATCGACAGCGCGTTTTTGCATTATTTTGTTACATGCAGAGATGAATTGCCGATTTAACAAGCACTTTTCTTTGCTTTTGCGCAAAccc                                                                                                                                                                                               <  2:214742/251‑1 (MQ=255)                                                 aaTACGCGCCAGGTGAATTTCCCTCTGGCGCGTAGAGTACGGGAATGGACATCAATATCCTTAAAGTAAATAAGACTATTCCTGACTATTATTGATAAGTTCTTTTAAACCCGCCCGTTAATTAACTCACCAGCTGAAATTCACAATAATTAAGTGATATCGACAGCGCGTTTTTGCATTATTTTGTTACATGCAGAGATGAATTGCCGATTTAACAAGCACTTTTCTTTGCTTTTGCGCAAACCCGCTgg                                                                                                                                                                                          <  2:290522/251‑1 (MQ=255)                                                    aCGCGCCATGTGAATTTCCCTCTGGCGCGTAGAGTACGGGAATGGACATCAATATCCTGAAAGTAAATAATACTATTCCTGACGATTATTGATAAGTTCGTTGAAACCCGCCCGTTAATTAACTCACCAGCTGAAATTCACAATAATGAAGTGATATCGACAGCGCGTTTTTGCATTATTTTGTTACATGCATAGATGAAGTGCCGATTTAACAAGCACTTTTCTTTGCTTTTGCGCAAACCCGCTGGCAt                                                                                                                                                                                       <  2:282891/251‑1 (MQ=255)                                                                   ttCCCTCTGGCGCGTAGAGTACGGGAATGGACATCAATATCCTTAAAGTAAATAAGACTATTCCTGACTATTATTGATAAGTTCTTTTAAACCCGCCCGTTAATTAACTCACCAGCTGAAATTCACAATAATTAAGTGATATCGACAGCGCGTTTTTGCATTATTTTGTTACATGCAGAGATGAATTGCCGATTTAACAAGCACTTTTCTTTGCTTTTGCGCAAACCCGCTGGCATCAAGCGCCACACAg                                                                                                                                                                         <  2:150198/250‑1 (MQ=255)                                                                             ggcgTAGAGTACGTGAATGGACATCAATATCCTTAAAGTAAATAAGACTATTCCTGACTATTATTGATAAGTTCTTTTAAACCCGCCCGTTAATTAACTCACCAGCTGAAATTCACAATAATTAAGTGATATCGACAGCGCGTTTTTGCATTATTTTGTTACATGCAGAGATGAATTGCCGATTTAACAAGCACTTTTCTTTGCTTTTGCGCAAACCCGCTGGCATCAAGCGCCACACAGACGCAACaggg                                                                                                                                                              <  2:354659/250‑4 (MQ=255)                                                                                   gagTACGGGAATGGAAATCAATATCCTCAAAGTAAATAAGACTATTCCTGACTATTATTGATAAGTTCTTTTAAACCCGCCCGTTAATTAACTCACCAGCTGAAATTCACAATAATTAAGTGATATCGACAGCGCGTTTTTGCATTATTTTGTTACATGCAGAGATGAATTGCCGATTTAACAAGCACTTTTCTTTGCTTTTGCGCAAACCCGCTGGCATCAAGCGCCACACAGACGCAACAGGGACTGtt                                                                                                                                                        <  2:326671/251‑1 (MQ=255)                                                                                   gagTACAGGAATGGACATCAATATCCTTAAAGTAAATAAGACTATTCCTGACTATTATTGATAAGTTCTTTTAAACCCGCCCGTTAAGTAACTCACCAGCTGAAATTCTCAATAATTAAGTGATAGCGACAGCGCGTTTTTGCATTATTTTGTTACATGCAGAGATGAAGTGCCGATTTAACAAGCACTTTTCTTTGCTTTTGCGCAAACCCGCTGGCATCAAGCGCCACACAGACGCAACAGGGACTGtt                                                                                                                                                        <  2:393297/251‑1 (MQ=255)                                                                                        cGGGAATGGACATCAATATCCTTAAAGTAAATAAGACTATTCCTGACTATTATTGATAAGTTCTTTTAAACCCGCCCGTTAATTAACTCAACAGCTGAAATTCCCAATAATTAAGTGATATCGACAGCGCGTTTTTGCATTATTTTGTTACATGCAGCGATGAATTGCCGATTTAACAAGCACTTTTCTTTGCTTTTGCGCAAACCCGCTGGCATCAAGCGCCACACAGACGCAACAGGGACTGTTAACCg                                                                                                                                                   >  2:402020/1‑251 (MQ=255)                                                                                               ggACATCAATATCCTTAAAGTAAATAAGACTATTCCTGACTATTATTGATAAGTTCTTTTAAACCCGCCCGTTAATTAACTCACCAGCTGAAATTCACAATAATTAAGTGATATCGACAGCGCGTTTTTGCATTATTTTGTTACATGCAGAGATGAATTGCCGATTTAACAAGCACTTTTCTTTGCTTTTGCGCAAACCCGCTGGCATCAAGCGCCACACAGACGCAACAGGGACTGTTAACCGGGGAAGa                                                                                                                                            <  2:46942/251‑1 (MQ=255)                                                                                                gACATCAATATCCTTAAAGTAAATAAGACTATTCCTGACTATTATTGATAAGTTCTTTTAAACCCGCCCGTTAATTAACTCACCAGCTGAAATTCACAATAATTAAGTGATATCGACAGCGCGTTTTTGCATTATTTTGTTACATGCAGAGATGAATTGCCGATTTAACAAGCACTTTTCTTTGATTTTGCGCAAACCCGCTGGCATCAAGCGCCACACAGACGCAACAGGGACTGTTAACCGGGGAAGat                                                                                                                                           <  1:205287/251‑1 (MQ=255)                                                                                                 aCATCAATATCCTTAAAGTAAATAAGACTATTCCTGACTATTATTGATAAGTTCTTTTAAACCAGCCCGTTATTTAACTCACCAGCTGAACTTCACAATAATTAAGTGATATCGACAGCGCGTTTTTGCATTATTTTGTTACATGCAGAGATGAATTGCCGATTTAAAAAAAACTTTTCTTTGCTTTTACGCAAACCCGCTGGCATAACACGCCACACAGACGCAACAGGGACTGTTACACGGGGAAGat                                                                                                                                           >  2:187326/1‑250 (MQ=255)                                                                                                                aaGTAAATAAGACTATTCCTGACTATTATTGATAAGTTCTTTTAAACCAGCCCGTTAATTAACTCACCAGCTGAAATTCACAATAATTAAGTGATATCGACAGCGCGTTTTTGCATTATTTTGTTACATGCAGAGATGAATTGCCGATTTAACAAGCACTTTTCTTTGCTTTTGCGCAAACCCGCTGGCATCAAGCGCCACACAGACGCAACAGGGACTGTTAACCGGGGAAGATATGTCCTAAAATGCCg                                                                                                                           >  2:215035/1‑251 (MQ=255)                                                                                                                    aaaTAAGACTATTCCTGACTATTATTGATAAGTTCTTTTAAACCCGCCCGTTAATTAACTCACCAGCTGAAATTCACAATAATTAAGTGATATCGACAGCGCGTTTTTGCATTATTTTGTTACATGCAGAGATGAATTGCCGATTTAACAAGCACTTTTCTTTGCTTTTGCGCAAACCCGCTGGCATCAAGCGCCACACAGACGCAACAGGGACTGTTAACCGGGGAAGATATGTCCTAAAATGCCGCTcg                                                                                                                       <  1:32810/251‑1 (MQ=255)                                                                                                                                tCCTGACTATTATTGATAAGTTCTTTTAAACCCGCCCGTTAATTAACTCACCAGCTGAAATTCACAATAATTAAGTGATATCGACAGCGCGTTTTTGCATTATTTTGTTACATGCAGAGATGAATTGCCGATTTAACAAGCACTTTTCTTTGCTTTTGCGCAAACCCGCTGGCATCAAGCGCCACACAGACGCAACAGGGACTGTTAACCGGGGAAGATATGTCCTAAAATGCCGCTCGCGTCGCAAACt                                                                                                            <  1:118995/250‑1 (MQ=255)                                                                                                                                    gACTATTATTGATAAGTTCTTTTAAACCCGCCCGTTAATTAACTCACCAGCTGAAATTCACAATAATTAAGTGATATCGACAGCGCGTTTTTGCATTATTTTGTTACATGCAGAGATGAATTGCCGATTTAACAAGCACTTTTCTTTGCTTTTGCGCAAACCCGCTGGCATCAAGCGCCACACAGACGCAACAGGGACTGTTAACCGGGGAAGATATGTCCTAAAATGCCGCTCGCGTCGCAAACTGacac                                                                                                       >  1:184918/1‑251 (MQ=255)                                                                                                                                        attattGATAAGTTCTTTTAAACCCGCCCGTTAATTAACTCACCAGCTGAAATTCACAATAATTAAGTGATATCGACAGCGCGTTTTTGCATTATTTTGTTACATGCAGAGATGAATTGCCGATTTAACAAGCACTTTTCTTTGCTTTTGCGCAAACCCGCTGGCATCAAGCGCCACACAGACGCAACAGGGACTGTTAACCGGGGAAGATATGTCCTAAAATGCCGCTCGCGTCGCAAACTGACACttt                                                                                                    >  1:146121/1‑250 (MQ=255)                                                                                                                                          tattGATAAGTTCTTTTAAACCCGCCCGTTAATTAACTCACCAGCTGAAATTCACAATAATTAAGTGATATCGACAGCGCGTTTTTGCATTATTTTGTTACATGCAGAGATGAATTGCCGATTTAACAAGCACTTTTCTTTGCTTTTGCGCAAACCCGCTGGCATCAAGCGCCACACAGACGCAACAGGGACTGTTAACCGGGGAAGATATGTCCTAAAATGCCGCTCGCGTCGCAAACTGACACTTtat                                                                                                  <  1:461272/250‑1 (MQ=255)                                                                                                                                               ataagttcTTTTAAACCCGCCCGTTAATTAACTCACCAGCTGAAATTCACAATAATTAAGTGATATCGACAGAGAGTTTTTGCATTATTTTGTTACATGCAGAGATGAATTGCCGATTTAACAAGCACTTTTCTTTGCTTTTGCGCAAACCCGCTGACATCAAGCGCCACACAGACGCAACAGGGACTGTTAACCGGGGAAGATATGTCCTAAAATGCTGATCGCGTCGAGAACTGACACTTTATATTTg                                                                                             >  1:447886/8‑250 (MQ=255)                                                                                                                                                           aaaCCCGCCCGTTAATTAACTCACCAGCTGAAATTCACAATAATTAAGTGATATCGACAGCGCGTTTTTGCATTATTTTGTTACATGCAGAGATGAATTGCCGATTTAACAAGCACTTTTCTTTGCTTTTGCGCAAACCCGCTGGCATCAAGCGCCACACAGACGCAACAGGGACTGTTAACCGGGGAAGATATGTCCTAAAATGCCGCTCGCGTCGCAAACTGACACTTTATATTTGCTGTGGAAAATa                                                                                 <  1:114310/250‑1 (MQ=255)                                                                                                                                                                     gTTAATTAACTCACCAGCTGAAATTCACAATAATTAAGTGATATCGACAGCGCGTTTTTGCATTATTTTGTTACATGCAGAGATGAATTGCCGATTTAACAAGCACTTTTCTTTGCTTTTGCGCAAACCCGCTGGCATCAAGCGCCACACAGACGCAACAGGGACTGATAACCGGGGAAGATATGTCCTAAAATGCCGCTCGCGTCGCAAACTGACACTTTATATTTGCTGTGGAAAATAGTGAGTCAttt                                                                      <  2:41068/251‑1 (MQ=255)                                                                                                                                                                            aaCTCACCAGCTGAAAGTCACAATAATTAAGTGATATCGACAGCGCGTTTTTGCATTATTTTGTTACATGCAGAGATGAATTGCCGATTTAACAAGCACTTTTCTTTGCTTTTGCGCAAACCCGCTGGCATCAAGCGCCACACAGACGCAACAGGGACTGTTAACCGGGGAAGATATGTCCTAAAATGCCGCTCGCGTCGCAAACTGACACTTTATATTTGCTGTGGAAAATAGTGAGTCATTTTAAAACg                                                               <  2:383540/251‑1 (MQ=255)                                                                                                                                                                                    aGCTGAAATTCACAATAATTAAGTGATATCGACAGCGCGTTTTTGCATTATTTTGTTACATGCAGAGATGAATTGCCGATTTAACAAGCACTTTTCTTTGCTTTTGCGCAAACCCGCTGGCATCAAGCGCCACACAGACGCAACAGGGACTGTTAACCGGGGAAGATATGTCCTAAAATGCCGCTCGCGTCGCAAACTGACACTTTATATTTGCTGTGGAAAATAGTGAGTCATTTTAAAACGGTGATGAc                                                       >  2:142907/1‑251 (MQ=255)                                                                                                                                                                                        gAAATTCACAATAATTAAGTGATATCGACAGCGCGTTTTTGCATTATTTTGTTACATGCAGAGATGAATTGCCGATTTAACAAGCACTTTTCTTTGCTTTTGCGCAAACCCGCTGGCATCAAGCGCCACACAGACGCAACAGGGACTGTTAACCGGGGAAGATATGTCCTAAAATGCCGCTCGCGTCGCAAACTGACACTTTATATTTGCTGTGGAAAATAGTGAGTCATTTTAAAACGGTGATGACGAt                                                    <  1:402020/250‑1 (MQ=255)                                                                                                                                                                                                     ataaaGTGCTATCAACAGCGCGTTTTTGCATTACTTTGTTACATGCAGCGGTGAATTGCCGATTTAACACGCACTTTTCATCACTATTGCGCAAACCCGCTGGCCTCATGCGCCACACAGACGCAACTGGGACTGTTAACAGTGGAAGCTATGTCCTAAACTGCCGCCCGCCTCGCAAACTGACACTTTATATTTGCTGTGGACTATAGTGGGTCATTTTAAAACGGTGAGAACGAGGAGGGATTTATTCt                                      >  2:18535/4‑251 (MQ=255)                                                                                                                                                                                                      ttAAGTGATATCGACAGCGCGTTTTTGCCTTATTTTGTTACATGCAGAGATGAATTGCCGATTTAACAAGCACTTTTCTTTGCTTTTGCGCAAACCCGCTGGCATCAAGCGCCACACAGACGCAACAGGGCCTGTTAACCGGGGAAGATATGTCCTAAAATGCCGCTCGCGTCGCAAACTGACACTTTATATTTGCTGTGGAAAATAGTGAGTCATTTTAAACCGGTGATGACGATGAGGGATTTTTTCtt                                     >  2:174073/1‑251 (MQ=255)                                                                                                                                                                                                                     aGCGCGTTTTTGCATTATTTTGTTACATGCAGAGATGAATTGCCGATTTAACAAGCACTTTTCTTTGCTTTTGCGCAAACCCGCTGGCATCAAGCGCCACACAGACGCAACAGGGACTGTTAACCGGGGAAGATATGTCCTAAAATGCCGCTCGCGTCGCAAACTGACACTTTATATTTGCTGTGGAAAATAGTGAGTCATTTTAAAACGGTGATGACGATGAGGGATTTTTTCTTACAGCTATTCATAAc                      >  1:4279/1‑251 (MQ=255)                                                                                                                                                                                                                        gcatttttGCATTATTTTGTTACATGCAGAGATGAATTGACGATTTAACAAGCACTTTTCTTTGATTTTGCGCAAACCCGCTGGAATCAAGCGCCACACAGACGCACCAGGGACTGTTAACAGGGGACGATATGTCCTAAAATGCCGCTCGCGTCGCAAACTGACACTTTATATTTGCTGTGGAAAATAGTGAGTCATTTTAAAACGGTGATGACGATGAGGGATTTTTTCTTACAGCTATTCATAATGtt                   >  2:238743/4‑251 (MQ=255)                                                                                                                                                                                                                                 cATTATTTTGGTACATGCAGAGATGAATTGCCGATTTAACAAGCACTTTTCTTTGCTTTTGCGCAAACCCGCTGGCATCAAGCGCCACACAGACGCAACAGGGACTGTTAACCGGGGAAGATATGTCCTAAAATGCCGCTCTCGTCGCAAACTGACACTTTATATTTGCTGTGGAAAATAGTGAGTCATTTTAAAACGGTGATGACGATGAGGGATTTTTTCTTACAGCTATTCATAACGTTAATTTGCt           <  2:76863/250‑1 (MQ=255)                                                                                                                                                                                                                                        ttGTTACATGCAGAGATGAATTGCCGATTTAACAAGCACTTTTCTTTGCTTTTGCGCAAACCCGCTGGCATCAAGCGCCACACAGACGCAACAGGGACTGTTAACCGGGGAAGATATGTCCTAAAATGCCGCTCGCGTCGCAAACTGACACTTTATATTTGCTGTGGAAAATAGTGAGTCATTTTAAAACGGTGATGACGATGAGGGATTTTTTCTTACAGCTATTCATAACGTTAATTTGCTGCGCACg    <  1:458294/250‑1 (MQ=255)                                                                                                                                                                                                                                         tGTTACATGCAGAGATGAATTGCCGATTTAACAAGCACTTTTCTTTGCTTTTGCGCAAACCCGCTGGCATCAAGCGCCACACAGACGCAACAGGGACTGTTAACCGGGGAAGATATGTCCTAAAATGCCGCTCGCGTCGCAAACTGACACTTTATATTTGCTGTGGAAAATAGTGAGTCATTTTAAAACGGTGATGACGATGAGGGATTTTTTCTTACAGCTATTCATAAAGTTAATTTGCTGCGCACGtt  >  2:386359/1‑251 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                               AATCTCTATCGCCGCGGCGTGCTCAGCCGTAGCTAAGCCGCGAACAAAAATACGCGCCAGGTGAATTTCCCTCTGGCGCGTAGAGTACGGGACTGGACATCAATATGCTTAAAGTAAATAAGACTATTCCTGACTATTATTGATAAATGCTTTTAAACCCGCCCGTTAATTAACTCACCAGCTGAAATTCACAATAATTAAGTGATATCGACAGCGCGTTTTTGCATTATTTTGTTACATGCGGCGATGAATTGCCGATTTAACAAACACTTTTCTTTGCTTTTGCGCAAACCCGCTGGCATCAAGCGCCACACAGACGTAACAAGGACTGTTAACCGGGGAAGATATGTCCTAAAATGCCGCTCGCGTCGCAAACTGACACTTTATATTTGCTGTGGAAAATAGTGAGTCATTTTAAAACGGTGATGACGATGAGGGATTTTTTCTTACAGCTATTCATAACGTTAATTTGCTTCGCACGTT  >  NC_000913/88997‑89479

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑