| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 66,092 | A→G | F153F (TTT→TTC) | araD ← | L‑ribulose‑5‑phosphate 4‑epimerase AraD |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 66,092 | 0 | A | G | 98.2% | 178.7 / ‑4.2 | 57 | F153F (TTT→TTC) | araD | L‑ribulose‑5‑phosphate 4‑epimerase AraD |
| Reads supporting (aligned to +/- strand): ref base A (0/1); new base G (32/24); total (32/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.39e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.85e-01 | |||||||||||
ATACAGTCATTACTGCCCGTAATATGCCTTCGCGCCATGCTTACGCAGATAGTGTTTATCCAGCAGCGTTTGCTGCATATCCGGTAACTGCGGCGCTAACTGACGGCAGAATATCCCCATATAAGCGACCTCTTCCAGCACGATGGCGTTATGCACCGCATCTTCGGCATTTTTGCCCCATGCAAACGGGCCGTGGGAATGGACCAGAACGCCGGGCATTTGCGCTGCATCGATACCCTGTTTTTCAAAGGTTTCTACGATGACGTTACCGGTTTCCCACTCATATTCGCCGTTGATTTCTGCGTCGGTCATTTTGCGGGTGCA‑GGGAATGGTGCCGTAGAAATAGTCGGCGTGGGTGGTGCCGGTTGCTGGAATCGACTGACCCGCCTGCGCCCAGATGGTGGCGTGGCGCGAGTGCGTATGCACAATGCCGCCAATGGAGGGGAATGCCTGATAGAGCAGCCGGTGAGTTGGCGTGTCGGAGGAGGGCT > NC_000913/65846‑66336 | ataCAGTCATTACTGCCCGTAATATGCCTTCGCGCCATGCTTACGCAGATAGTGTTTATTCAGCAGCGTTTGCTGCATATCCGGTAACTGCGGCGCTAACTGACGGCAGAATATCCCCATATAAGCGACCTCTTCCAGCACGATGGCGTTATGCACCGCATCTTCGGCATTTTTGCCC‑ATGCAAATGGGCCGTGAGAATGGACCAGGACGCCGGGCATTTGCGCTGCATCGATACCCTGTTTTTCGAAGGt < 1:356135/251‑1 (MQ=255) aGTCATTACTGCCCGTAATATGCCTTCGCGCCATGCTTACGCAGATAGTGTTTATTCAGCAGCGTTTGCTGCATATCCGGTAACTGCGGCGCTAACTGACGGCAGAATATCCCCATATAAGCGACCTCTTCCAGCACGATGGCGTTATGCACCGCATCTTCGGCATTTTTGCCC‑ATGCAAATGGGCCGTGAGAATGGACCAGGACGCCGGGCATTTGCGCTGCATCGATACCCTGTTTTTCGAAGGTTTCt < 1:12742/251‑1 (MQ=255) gCCCGTAATATGCCTTCGCGCCATGCTTACGCAGCTAGTGTTTATTCAGCAGCGTTTGCTGCATATCCGGTAACTGCGGCGCTAACTGACGGCAGAATATCCCCATATAAGCGACCTCTTCCAGCCCGATGGCGTTATGCACCGCATCTTCGGCATTTTTGCCC‑ATGCAAATGGGCCGTGAGAATGGACCAGGACGCCGGGCATTTGCGCTGCATCGATACCCTGTTTTTCGAAGGTTTCTACGATGACGt > 2:438626/1‑251 (MQ=255) gTAATATGCCTTCGCGCCATGCTTACGCAGATAGTGTTTATTCAGCAGCGTTTGCTGCATATCCGGTAACTGCGGCGCTAACTGACGGCAGAATATCCCCATATAAGCGACCTCTTCCAGCACGATGGCGTTATGCACCGCATCTTCGGCATTTTTGCCC‑ATGCAAATGGGCCGTGAGAATGGACCAGGACGCCGGGCATTTGCGCTGCATCGATACCCTGTTTTTCGAAGGTTTCTACGATGACGTTAcc > 1:310626/1‑251 (MQ=255) atatGCCTTCGCGCCATGCTTACGCAGATAGAGTTTATTCAGCAGCGTTTGCTGCATATCCGGTAACTGCGGCGCTAACTGACGGCAGAATATCCCCATATAAGCGACCTCTTCCAGCACGATGGCGTTATGCACCGCATCTTCGGCATTTTTGCCC‑ATGCAAATGGGCCGTGAGAATGGACCAGGACGCCGGGCATTTGCGCTGCATAGATACCCTGTTTTTCGAAGGTTTCTACGATGACGTTACCGGt > 1:412876/1‑251 (MQ=255) gcaaTGCTTACGCAGATAGTGTTGATTCAGCAGCGTTTGCTGCATATCCGGTAACTGCGGCGCTAACTGACGGCAGAATATCCCCATATAAGCGACCTCTTCCAGCACGATGGCGTTATGCACCGCATCTTCGGCATTTTTGCCC‑ATGCAAATGGGCCGTGAGAATGGACCAGGACGCCGGGCATTTGAGCTGCATCGATACCCTGTTTTTCGAAGGTTTCTACGATGACGTTACCGGTTTCCCACTCata < 1:150033/248‑1 (MQ=255) gcCATGCTTACGCAGATAGTGTTTATTCAGCAGCGTTTGCTGCATATCCGGTAACTGCGGCGCTAACTGACGGCAGAATATCCCCATATAAGCGACCTCTTCCAGCACGATGGCGTTATGCACCGCATCTTCGGCATTTTTGCCC‑ATGCAAATGGGCCGTGAGAATGGACCAGGACGCCGGGCATTTGCGCTGCATCGATACCCTGTTTTTCGAAGGTTTCTACGATGACGTTACCGGTTTCCCACTCata < 1:64720/251‑1 (MQ=255) tGCTTACGCAGATAGTGTTTATTCAGCAGCGTTTGCTGCATATCCGGTAACTGCGGCGCTAACTGACGGCAGAATATCCCCATATAAGCGACCTCTTCCAGCACGATGGCGTTATGCACCGCATCTTCGGCATTTTTGCCC‑ATGCAAATGGGCCGTGAGAATGGACCAGGACGCCGGGCATTTGCGCTGCATCGATACCCTGTTTTTCGAAGGTTTCTACGATGACGTTACCGGTTTCCCACTCATATTc > 1:199514/1‑250 (MQ=255) gCTTACGCAGATAGTGTTTATTCAGCAGCGTTTGCTGCATATCCGGTAACTGCGGCGCTAACTGACGGCAGAATATCCCCATATAAGCGACCTCTTCCAGCACGATGGCGTTATGCACCGCATCTTCGGCATTTTTGCCC‑ATGCAAATGGGCCGTGAGAATGGACCAGGACGCCGGGCATTTGCGCTGCATCGATACCCTGTTTTTCGAAGGTTTCTACGATGACGTTACCGGTTTCCCACTCATATTca < 1:5153/250‑2 (MQ=255) gCTTACGCAGATAGTGTTTATTCAGCAGCGTTTGCTGAATATCCGTAAACTGCGGCGCTAACTGACGGCAGAATACCCCCATATAAGCGACCTCTTCCAGCACGATGGCGTTATGCACCGCATCTGCGGCATTTTTGCCC‑ATGCAAATGGGCCGTTAGAATGGACCAGGACGCCGGGCATTTGCGCTGCATCGATACCCTGTTTTTCGAAGGTTTCTACGATGACGTTACCGGTTTCCCACTCATATTca < 2:358278/250‑2 (MQ=255) cTTACGCAGATAGTGTTTATTCAGCAGCGTTTGCTGCATATCCGGTAACTGCGGCGCTAACTGACGGCAGAATATCCCCATATAAGCGACCTCTTCCAGCACGATGGCGTTATGCACCGCATCTTCGGCATTTTTGCCC‑ATGCAAATGGGCCGTGAGAATGGACCAGGACGCCGGGCATTTGCGCTGCATCGATACCCTGTTTTTCGAAGGTTTCTACGATGACGTTACCGGTTTCCCACTCATATTcacc > 1:235404/1‑248 (MQ=255) ttACGCAGATAGTGTTTATTCAGCAGCGTTTGCTGCATATCCGGTAACTGCGGCGCTAACTGACGGCAGAATATCCCCATATAAGCGACCTCTTCCAGCACGATGGCGTTATGCACCGCATCTTCGGCATTTTTGCCC‑ATGCAAATGGGCCGTGAGAATGGACCAGGACGCCGGGCATTTGCGCTGCATCGATACCCTGTTTTTCGAAGGTTTCTACGATGACGTTACCGGTTTCCCACTCATATTCACCg > 2:38959/1‑251 (MQ=255) aCGCAGATAGTGTTTATTCAGCAGCGTTTGCTGCAGAACCGGTAACTGCGGCGCTAACTGACGGCAGAATATCCCCATATAAGAGACCTCTTCCAGCACGATGGCGTTATGCACCGAATCTTCGGCATTTTTGCCC‑ATGCAAATGGGCCGTGAGAATGGACCAGGACGCCGGGCATTTGCGCTGCATCGATACCCTGTTTTTAGAAGGTTTCTACGATGACGTTACCGGTTTCCCGCTCATATTcacagtt > 2:41979/1‑245 (MQ=255) aGATAGTGTTTATTCAGCAGCGTTTGCTGCATATCCGGTAACTGCGGCGCTAACTGACGGCAGAATATCCCCATATAAGCGACCTCTTCCAGCACGATGGCGTTATGCACCGCATCTTCGGCATTTTTGCCC‑ATGCAAATGGGCCGTGAGAATGGACCAGGACGCCGGGCATTTGCGCTGCATCGATACCCTGTTTTTCGAAGGTTTCTACGATGACGTTACCGGTTTCCCACTCATATTCACCGTTGAtt < 1:428399/251‑1 (MQ=255) gtgtTTATTCAGCAGCGTTTGCTGCATATCCGGTAACTGCGGCGCTAACTGACGGCAGAATATCCCCATATAAGCGACCTCTTCCAGCACGATGGCGTTATGCACCGCATCTTCGGCATTTTTGCCC‑ATGCAAATGGGCCGTGAGAATGGACCAGGACGCCGGGCATTTGCGCTGCCTCGATACCCTGTTTTTCGAAGGTTTCTACGATGACGTTACCGGTTTCCCACTCATATTCACCGTTGATTTCTGc > 2:124492/1‑251 (MQ=255) tgtTTATTCAGCAGCGTTTGCTGCATATCCGGTAACTGCGGCGCTAACTGACGGCAGAATATCCCCATATAAGCGACCTCTTCCAGCACGATGGCGTTATGCACCGCATCTTCGGCATTTTTGCCC‑ATGCAAATGGGCCGTGAGAATGGACCAGGACGCCGGGCATTTGCGCTGCATCGTTACCCTGTTTTTCGCAGGTTTCTACGATTGCGTTACCGGTTTCCCACTCCTATTCACCTTTGATTTCTGcc > 2:412342/1‑250 (MQ=255) tgtTTATTCAGCAGCGTTTGCTGCATATCCGGTAACTGCGGCGCTAACTGACGGCAGAATATCCCCATATAAGCGACCTCTTCCAGCACGATGGCGTTATGCACCGCATCTTCGGCATTTTTGCCC‑ATGCAAATGGGCAGTGAGAATGGACCAGGACGCCGGGCATTTGCGCTGCATCGATACCCTGTTTTTCGAAGGTTTCTACGATGACGTTACCGGTTTCCCACTCATATTCACCGTTGATTTCTGCg > 1:129173/1‑251 (MQ=255) attcagcagCGTTTGCTGCATATCCGGTAACTGCGGCGCTAACTGACGTCAGAATTTCACCATATAAGCGACCTCTTCCACCACGATGGCGTTATGCACCGCATCTTCGACCTTTTTGCCC‑ATGCAAATGGGCCGCGAGAATGGACCAGGACGCCGGGCATTTGCGCTGCATCGATACCCTGTTTTTCGAAGGTTTCTACGATGCCGTTACCGGTTTCCCACTCATATTCACCGTTGATTTCTGCGTCGGt > 1:263057/4‑251 (MQ=255) tcagcagCGTTTGCTGAATATCCGGTAACTGCGGCGCTAACTGACGGCAGACTATCCACATATAAGCGACCTCTTCCAGCACGATGGCGTTATGCACAGCATCTTCGGCATTTTTGCCC‑ATGCAAATGGGCCGTGAGAATGGACCAGGACGCCGGGCATTTGCGCTGCATCGATACCCTGTTTTTCGAAGGTTTCTACGATGACGTTACCGGTTTCCCACTCATATTCACCGTTGATTTCTGCGTCGGTc > 1:297911/2‑250 (MQ=255) gctgcATATCCGGTAACTGCGGCGCTAACTGACGGCAGAATATCCCCATATAAGCGACCTCTTCCAGCACGATGGCGTTATGCACCGCATCTTCGGCATTTTTGCCC‑ATGCAAATGGGCCGTGAGAATGGACCAGGACGCCGGGCATTTGCGCTGCATCGATACCCTGTTTTTCGAAGGTTTCTACGATGACGTTACCGGTTTCCCACTCATATTCACCGTTGATTTCTGCGTCGGTCATTTTGCGGGTg < 1:38959/250‑1 (MQ=255) tCCGGTAACTGCGGCGCTAACTGACGGCAGCATATCCCCATATAAGCGACCTCTTCCAGCACGATGGCGTTATGCACCGCATCTTCGGCATTTTTGCCC‑ATGCAAATGGGCCGTGAGAATGGACCAGGACGCCGGGCATTTGCGCTGCATCGATACCCTGTTTTTCGAAGGTTTCTACGATGACGTTACCGGTTTCCCACTCATATTCCCCGTTGATTTCTGCGTCGGTCATTTTGCGGGTGCG‑GGGaa > 2:325874/1‑249 (MQ=255) gcTAACTGACGGCAGAATATCCCCATATAAGCGACCTCTTCCAGCACGATGGCGTTATGCACCGCATCTTCGGCATTTTTGCCC‑ATGCAAATGGGCCGTGAGAATGGACCAGGACGCCGGGCATTTGCGCTGCATCGATACCCTGTTTTTCGAAGGTTTCTACGATGACGTTACCGGTTTCCCACTCATATTCACCGTTGATTTCTGCGTCGGTCATTTTGCGGGTGCA‑GGGAATGGTGCCGTCGAAAt > 1:251870/1‑249 (MQ=255) aactgcgagccaacgatCCCCATATAAGCGACCTCTTCCAGCACGACGGAGTTATGCACCGCATCCTCGGCATTGTTGCCC‑ATGCAAATGGGCCGTGAGAAGGGTCCAGGACGCCTGGCAATTGCGCTGCCTCGATACCCTGTTATTCGAAGGTATCTACGATGACGCGATCGGGTTCCCGCGCATATTCACCGTTGATTTCTACGTCTGTCATTTTGCGG‑AGCAAGGGAATGGTACAGTAGAAATGGTCg > 2:106129/16‑251 (MQ=255) aaCTGACGGCAGAATATCCCCATATAAGCGACCTCTTCCAGCACGATGGCGTTATGCACCGCATCTTCGGCATTTTTGCCC‑ATGCAAATGGGCCGTGAGAATGGACCAGGACGCCGGGCATTTGCGCTGCATCGATACCCTGTTTTTCGAAGGTTTCTACGATGACGTTACCGGTTTCCCACTCATATTCACCGTTGATTTCTGCGTCGGTCATTTTGCGGGTGCA‑GGGAATGGTGCCGTAGAAATAGt < 1:47674/249‑1 (MQ=255) aaCTGACGGCAGAATATCCCCATATAAGCGACCTCTTCCAGCACGATGGCGTTATGCACCGCATCTTCGGCATTTTTGCCC‑ATGCAAATGGGCCGTGAGAATGGACCAGGACGCCGGGCATTTGCGCTGCATCGATACCCTGTTTTTCGAAGGTTTCTACGATGACGTTACCGGTTTCCCACTCATATTCACCGTTGATTTCTGCGTCGGTCATTTTGCGGGTGCA‑GGGAATGGTGCCGTAGAAATAGTc < 1:445002/250‑1 (MQ=255) gACGGCAGAATATCCCCATATAAGCGACCTCTTCCAGCACGATGGCGTTATGCACCGCATCTTCGGCATTTTTGCCCCATGCAAACGGGCCGTGGGAATGGACCAGAACGCCGGGCATTTGCGCTGCATCGATACCCTGTTTTTCAAAGGTTTCTACGATGACGTTACCGGTTTCCCACTCATATTCGCCGTTGATTTCTGCGTCGGTCATTTTGCGGGTGCA‑GGGAATGGTGCCGTAGAAATAGTCGGc < 1:139186/250‑1 (MQ=255) gCAGAATATCCCCATATAAGCGACCTCTTCCAGCACGATGGCGTTATGCACCGCATCTTCGGCATTTTTGCCC‑ATGCAAATGGGCCGTGAGAATGGACCAGGACGCCGGGCATTTGCGCTGCATCGATACCCTGTTTTTCGAAGGTTTCTACGATGACGTTACCGGTTTCCCACTCATATTCACCGTTGATTTCTGCGTCGGTCATTTTGCGGGTGCA‑GGGAATGGTGCCGTAGAACTAGTCGGCgtgggg > 2:194296/1‑250 (MQ=255) aGAAAATCACCATATAAGCGACCTCTGCCAGAACGATGGCGTGATGCACCGCAGCTTCTGCATTTTTGCCC‑ATGCAAATGTGCCGTGAGAATGGACCAGGACGCCGGGCATTTGCGCTGCATCGATACCCTGTTTTTCGAAGGTTTCTACGATGACGTTACCGGTTTCCCTCTCATATTCACCGTTGATTGCTGCGTCGGTCATTTTGCGGGGGCA‑GGGAATGGTGCCGTAGAAATAGTCGGCttgggtgt < 2:93031/251‑2 (MQ=255) aaTATCCCCATATAAGCGACCTCTTCCAGCACGATGGCGTTATGCACCGCATCTTCGGCATTTTTGCCC‑ATGCAAATGGGCCGTGAGAATGGACCAGGACGCCGGGCATTTGCGCTGCATCGATACCCTGTTTTTCGAAGGTTTCTACGATGACGTTACCGGTTTCCCACTCATATTCACCGTTGATTTCTGCGTCGGTCATTTTGCGGGTGCA‑GGGAATGGTGCCGTAGAAATAGTCGGCGTGGgtggtg > 1:226186/1‑251 (MQ=255) atatCCCCATATAAGCGACCTCTTCCAGCACGATGGCGTTATGCACCGCATCTTCGGCATTTTTGCCC‑ATGCAAATGGGCCGTGAGAATGGACCAGGACGCCGGGCATTTGCGCTGCATCGATACCCTGTTTTTCGAAGGTTTCTACGATGACGTTACCGGTTTCCCACTCATATTCACCGTTGATTTCTGCGTCGGTCATTTTGCGGGTGCA‑GGGAATGGTGCCGTAGAAATAGTCGGCGTGGGTGGTGc > 1:228097/1‑251 (MQ=255) aGCGACCTCTTCCAGCACGATGGCGTTATGCACCGCATCGTCGGCATTTTTGCCC‑ATGCATATGGGCCGTGAGAATGGACCAGGACGCCGGGCATTTGCGCTGCATCGATACCCTGTTTTTCGAAGGTTTCTACGATGACGTTACCGGTTTCCCACTCATATTCACCGTTGATTTCTGCGTCGGTCATTGTGCGGGTGCA‑GGGAATGGTGCCGTAGAAATAGGCGGCGTGGGTGGTGCCGGTTGCTGGAAt < 2:328883/251‑1 (MQ=255) cgtccTCTTCCAGCACGGTGGCGTTTTGCACCGCACCTTCGGCATTTTTGCCC‑AGGCAAATGGGCCGTGAGAATGGACCAGTACGCCGGGCATTTGCGCTGCATCGATACCCTGTTTTTCGAAGGTTTCTACGAGGACGTTACCGGTTTCCCACTCATATTCACCGTTGATTTCTTCGTCGGTCATTTTGCGGGTGCA‑GGGAATGGTGCCGTAGAAATAGTCCGCGTGGGTGGTGCCGTTTGCTGGAATCg < 2:341356/248‑1 (MQ=255) ccTCTTCCAGCACGATGGCGTTATTCACCGCATCTTCGGCATTTTTGCCC‑ATGCAAATGGGCCGTGAGAATGGACCAGGACGCCGGGCATTTGCGCTGCATCGATACCCTGTTTTTCGAAGGTTTCTACGATGACGTTACCGGTTTCCCACTCATATTCACCGTTGATTTCTGCGTCGGTCATTTTGCGGGTGCA‑GGGAATGGTGCCGTAGAAATAGTCGGCGTGGGTGGTGCCGGTTGCTGGAATCGACt < 2:415002/251‑1 (MQ=255) cAGCACGATGGCGTTATGCACCGCATCTTCGGCATTTTTGCCC‑ATGCAAATGGGCCGTGAGAATGGACCAGGACGCCGGGCATTTGCGCTGCATCGATACCCTGTTTTTCGAAGGTTTCTACGATGACGTTACCGGTTTCCCACTCATATTCACCGTTGATTTCTGCGTCGGTCATTTTGCGGGTGCA‑GGGAATGGTGCCGTAGAAATAGTCGGCGTGGGTGGTGCCGGTTGCTGGAATCGACTGGCCCGc > 1:170752/1‑251 (MQ=255) tGGCGTTATGCACCGCATCTTCGGCATTTTTGCCC‑ATGCAAATGGGCCGTGAGAATGGACCAGGACGCCGGGCATTTGCGCTGCATCGATACCCTGTTTTTCGAAGGTTTCTACGATGACGTTACCGGTTTCCCACTCa < 1:41307/139‑1 (MQ=255) tGGCGTTATGCACCGCATCTTCGGCATTTTTGCCC‑ATGCAAATGGGCCGTGAGAATGGACCAGGACGCCGGGCATTTGCGCTGCATCGATACCCTGTTTTTCGAAGGTTTCTACGATGACGTTACCGGTTTCCCACTCa > 2:41307/1‑139 (MQ=255) tGGCGTTATGCACCGCATCTTCGGCATTTTTGCCC‑ATGCAAATGGGCCGTGAGAATGGACCAGGACGCCGGGCATTTGCGCTGCATCGATACCCTGTTTTTCGAAGGTTTCTACGATGACGTTACCGGTTTCCCACTCATATTCACCGTTGATTTCTGCGTCGGTCATTTTGCGGGTGCA‑GGGAATGGTGCCGTAGAAATAGTCGGCGTGGGTGGTGCCGGTTGCTGGAATCGACTGGCCCGCCTGCGccc > 1:157992/1‑251 (MQ=255) ggCGTTATGCACCGCATCTTCGGCATTTTTGCCC‑ATGCAAATGGGCCGTGAGAATGGACCAGGACGCCGGGCATTTGCGCTGCATCGATACCCTGTTTTTCGAAGGTTTCTACGATGACGTTACCGGTTTCCCACTCATATTCACCGTTGATTTCTGCGTCGGTCATTTTGCGGGTGCA‑GGGaa < 2:220875/184‑1 (MQ=255) ggCGTTATGCACCGCATCTTCGGCATTTTTGCCC‑ATGCAAATGGGCCGTGAGAATGGACCAGGACGCCGGGCATTTGCGCTGCATCGATACCCTGTTTTTCGAAGGTTTCTACGATGACGTTACCGGTTTCCCACTCATATTCACCGTTGATTTCTGCGTCGGTCATTTTGCGGGTGCA‑GGGaa > 1:220875/1‑184 (MQ=255) gCACCGCATCTTCGGCATTTTTGCCC‑ATGCAAATGGGCCGGGAGAATGGACCAGGACGCCGGGCATTTGCGCTGCATCGATACCCTGTTTTTCGAAGGTTTCTACGATGACGTTACCGGTTTCCCACTCATATTCACCGTTGATTTCTGCGTCGGTCATTTTGCGGGTGCA‑GGGAATGGTGCCGTAGAAATAGTCGGCGTGGGTGGTGCCGGTTGCTGGAATCGCCTGGCCCGCCTGCGCCGAGAtagtgg > 2:413474/1‑251 (MQ=255) ccGCATCTTCGGCATTTTTGCCCC‑TGCAAATGGGCCGTGCGAATGGACCAGGACGCCGGGCATTTGCGCTGCATCGATACCCTGTTTTTCGCAGGTTTCTACGATGACGTTACCGGTTTCCCACTCATATTCACCGTTGATTTCTGCGTCGGTCATTTTGCGGGTGCA‑GGGAATGGTTCCGTAGGAATAGTCTGCGGGGGTGGAGGCGGTTGATGGAATCGACTGGCCCGCCTGCGCCCAgtgagtggcgg > 2:54415/1‑241 (MQ=255) ccttcgtcattttGGCCCATGCAAATGGGCCGTGAGAATTGACCAGGACGCCGGGCATTTGCGCTGCATCGATACCCTGTTTTTCGAAGGTTTCTACGATGACGTTACCGGTTTCCCACTCATATTCACCGTTGATTTCTGCGTCGGTCATTTTGCGGGTGCA‑GGGAATGGTGCCGTAGAAATAGTCGGCGTGGGTGGTTCCGTTTGCTGGAATCGACTGGCCCGCCTGCGCCCAGATAGTGGCGTGgcgc < 1:272575/242‑1 (MQ=255) tCGGCATTTTTGCCC‑ATGCAAATGGGCCGTGAGAATGGTCCAGGACGCCGGGCATTTGCGCTGCATCGATACCCTGTTTTTCGAAGGTTTCTACGATGACGTTACCGGTTTCCCCCTCATATTCACCGTTGATTTCTGCGTCGGTCATTTTGCGGGTGCA‑GGGAATGGTGCCGTAGAAATAGTCGGCGTGGGTGGTGCCGGTTGCTGTAATCGACTGGCCCGCCTGCGCCCAGATAGtggcgtggcg < 1:364440/247‑1 (MQ=255) cGGCATTTTTGCCC‑ATGCAAATGGGCCGTGAGAATCGACCAGGACGCCGGGCATTTGCGCTGCATCGATACCCTGTTTTTCGAAGGTTTCTACGATGACGTTACCGGTTTCCCACTCATATTCACCGTTGATTTCTGCGTCGGTCATTTTGCGGGTGCA‑GGGAATGGTGCCGTAGAAATAGTCGGCGTGGGTGGTGCCGGTTGCTGGAATCGACTGGCCCGCCTGCGCCCAGATAGTGGCGTGGCGCGAg < 1:206653/250‑1 (MQ=255) aTTTTTGCCC‑ATGCAAATGGGCCGTGAGAATGGACCCGGACGCCGGGCATTTGCGCTGCATCGATACCCTGTTTTTCGAAGGTTTCTACGATGACGTTACCGGTTTCCCACTCATATTCACCGTTGATTTCTGCGTCGGTCATTTTGCGGGTGCA‑GGGAATGGTGCCGTAGAAATAGTCGGCGTGGGTGGTGCCGGTTGCTGGAATCGACTGGCCCGCCTGCGCCCAGATAgtggcgtggagggagggggt > 1:443435/1‑240 (MQ=255) gcccATGCAAATGGGCCGTGAGAATGGACCAGGACGCCGGGCATTTGCGCTGCATCGATACCTTGTTTTTCGAAGGTTTCTACGATGACGTTACCGGTTTCCCACTCATATTCACCGTTGATTTCTGCGTCGGTCATTTTGCGGGTGCA‑GGGAAGGGTGCCGTAGAAATAGTCGGCGTGGGTGGTGCCGGTGGCTGGAATCGTCTGGCCCGCCTGCGCCCAGATAGTGGCGTGGCGCGAGTGTGTGTGca < 2:334298/249‑1 (MQ=255) tGCAAATGGGCCGTGAGAATGGACCAGGACGCCGGGCATTTGCGCTGCATCGATACCCTGTTTTTCGAAGGTTTCTACGATGACGTTACCGGTTTCCCACTCATATTCACCGTTGATTTCTGCGTCGGTCATTTTGCGGGTGCA‑GGGAATGGTGCAGTAGAAATAGTCGGCGTGGGTGGTGCCGGTTGCTGGAATCGACTGGCCCGCCTGCGCCCAGATAGTGGCGTGGCGCGAGTgtgggtgcgcaatgc > 2:155069/1‑237 (MQ=255) aaaatgggCCGGGAGAATGGACCAGGACGCCGGGCATTTGCGCTGCATCGATTCCATGTTTTGCGACGGTTTCTACGATGACGTTACCGGTTTCCCACTCAAATTCTCCTGTGATTTCTGCGTCGGTCATTTCGCGGGTGCA‑GGGAATGGTGCCGTAGAAATAGTCGGCGTGGGTGGTGCCGTTTGCTGGAACCGACGGGTCCGCCTGCGCCCAGATAGTGGCGTGGCGCGAGTGTGTGTGCACAATgccg < 1:71902/246‑1 (MQ=255) ccGTGAGAATGGACCAGGACGCCGGGCATTTGCGCTGCATCGATACCCTGTTTTTCGAAGGTTTCTACGATGACGTTACCGGTTTCCCACTCATATTCACCGTTGATTTCTGCGTCGGTCATTTTGCGGGTGCA‑GGGAATGGTGCCGTAGAAATAGTCGGCGTGGGTGGTGCCGGTTGCTGGAATCGACTGGCCCGCCTGCGCCCAGATAGTGGCGTGGCGCGAGGGTGTGTGCACAATGCCGCCCATgg > 1:260598/1‑250 (MQ=255) gAATGGACCAGGACGCCGGGCATTTGCGCTGCATCGATGCCCTGTTTTTCGAAGGTTTCTACGATGACGTTACCGGTTTCCCACTCATATTCACCGTTGATTTCTGCGTCGGTCATTTTGCGGGTGCA‑GGGAATGGTGCCGTAGAAATAGTCGGCGTGGGTGGTGCCGGTTGCTGGAATCGACTGGCCCGCCTGCGCCCAGATAGTGGCGTGGCGCGAGTGTGTGTGCCCAATGCCGCCAATGGACGGGa > 1:418595/1‑250 (MQ=255) ggACCAGGACGCCGGGCATTTGCGCTTCATCGATACCCTGTTTTTCGAAGGTTTCTACGATGACGTTACCGGTTTCCCACTCATATTCACCGTTGATTGCTGCGTCGGTCATTTTGCGGGTGCA‑GGGAATGGTGCCGTAGAAATAGTCGGCGTGGGTGGTGCCGGTTGCTGGAATCGACTGGCCCGCCTGCGCCCAGATAGTGGCGTGGCGCGAGTGTGTGTGCACAATGCCGCCAATGGACGGGAATGcc < 1:335307/251‑1 (MQ=255) ccGGGCATTTGCGCTGCATCGATACCCTGTTTTTCGAAGGTTTCTACGATGACGTTACCGGTTTCCCACTCATATTCACCGTTGATTTCTGCGTCGGTCATTTTGCGGGTGCA‑GGGAATGGTGCCGTAGAAATAGTCGGCGTGGGTGGTGCCGGTTGCTGGAATCGACTGGCCCGCCTGCGCCCAGATAGTGGCGTGGCGCGAGTGTGTGTGCACAAAGCCGCCAATGGACGGGAATGCCTGATAGagc > 1:193923/1‑249 (MQ=255) cTGCATCGATACCCTGTTTTTCGAAGGTTTCTACGGTGACGTTACCGGTTTCCCACTCATATTCACCGTTGATTTCTGCGTCGGCCATTTTGCGGGTGCA‑GGGAATGGTGCCGTAGAAATAGTCGGCGTGGGTGGTGCCGGTTGCTGGCATCGACTGGCCCGCCTGCGCCCAGATGGTGGCGAGGCGGGAGTGTGTGTGCACAATGCCGCCAATGTACGGGCATTCCTGATAGAGCAGTCGGTGAGTTggg > 1:259780/1‑250 (MQ=255) aTCGATTCCCTGTTTTTCGAAGGTTTCTACGATGACTTTACCGGTTTCCCACTCATATTCACCGTTGATTTCTGCGTCGGTCATTTTGCGGGTGCA‑GGGAATGGTGCCGTAGAAATAGTCGGCGTGGGTGGTGCCGGTTGCTGGAATCGACTGGCCCGCCTGCGCCCAGATAGTGGCGTGGCGCGAGTGTGTGTGCACAATGCCGCCAATGGACGGGAATGCCTGATAGAGCAGTCGGTGAGTTGGCgtat < 1:128284/251‑3 (MQ=255) aTCGATACCCTGTTTTTCGAAGGTTTCTACGATGACGTTACCGTTTTCCCACTCATATTCACCGTTGATTTCTGCGTCGGTCATTTTGCGGGTGCA‑GGGAATGGTGCCGTAGAAATAGTCGGCGTGGGTGGTGCCGGTTGCTGGAATCGACTGGCCCGCCTGCGCCCAGATAGTGGCGTGGCGCGAGTGTGTGGGCACAATGCCCCCAATGGACGGGAATGCCTGATAGAGGCGGCGGTGAGTTggggtat > 2:198964/1‑246 (MQ=255) gATACCCTTTTTGTCGAAGTTTTCTACGATGCCTGTACCGTTTTCACACTCAGCTGCACCGTTGATTGCTGCGTCGGTTATTGTGCGGGTGCA‑GGGAATGGTGCCGTAGAAAGAGTCGGCGTGGGTGGTGCCGGTTGCTGGAATCGACTGGCCCGCCTGCGCCCAGATAGGGGCGTGGCGCGAGTGTGTGGGCACAATGCCGTCAATGGACTGGAATGCCTGATAGAGCAGTCGGTGAGTTGGCGTATCgt < 2:36664/251‑2 (MQ=255) tttttCGAAGGTTTCTACGATGACGTTACCGGTTTCCCACTCATATTCACCGTTGATTTCTGCGTCGGTCATTTTGCGGGTGCA‑GGGAATGGTGCCGTAGAAATAGTCGGCGTGGGTGGTGCCGGTTGCTGGAATCGACTGGCCCGCCTGCGCCCAGATAGTGGCGTGGCGCGAGTGTGTGTGCACAATGCCGCCAATGGACGGGAATGCCTGATAGAGCAGTCGGTGAGTTGGCGTATCGGGGGCGGGCt > 1:429037/1‑251 (MQ=255) | ATACAGTCATTACTGCCCGTAATATGCCTTCGCGCCATGCTTACGCAGATAGTGTTTATCCAGCAGCGTTTGCTGCATATCCGGTAACTGCGGCGCTAACTGACGGCAGAATATCCCCATATAAGCGACCTCTTCCAGCACGATGGCGTTATGCACCGCATCTTCGGCATTTTTGCCCCATGCAAACGGGCCGTGGGAATGGACCAGAACGCCGGGCATTTGCGCTGCATCGATACCCTGTTTTTCAAAGGTTTCTACGATGACGTTACCGGTTTCCCACTCATATTCGCCGTTGATTTCTGCGTCGGTCATTTTGCGGGTGCA‑GGGAATGGTGCCGTAGAAATAGTCGGCGTGGGTGGTGCCGGTTGCTGGAATCGACTGACCCGCCTGCGCCCAGATGGTGGCGTGGCGCGAGTGCGTATGCACAATGCCGCCAATGGAGGGGAATGCCTGATAGAGCAGCCGGTGAGTTGGCGTGTCGGAGGAGGGCT > NC_000913/65846‑66336 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |