Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,756,976	G→A	R273Q (CGA→CAA)	intA →	integrase IntA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,756,976	0	G	A	100.0%	134.7 / NA	46	R273Q (CGA→CAA)	intA	integrase IntA
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (30/16);  total (30/16)

CTGTTCCTAGTGTAAATATCGGAAAAGCTTTCGAGAAACCGCAAAAGAAAAACATGCCAAGCATCCGGCCGGATCAACTTCCGCAGCTAATGCACACCATGCGTACGGCAAGTATCAGCATGTCCACAAGATGCCTGTTCATGTGGCAACTTCTAACCATCACCCGCCCTGCCGAAGCTGCTGAGGCTCGATGGGATGAGATCGATTTCAATGCTAGCGAATGGAAAATTCCTGCAGCTCGAATGAAGATGAACCGGGACCATACGGTTCCACT‑ATCTGATGGGGCTCTTGCTATTCTGGAAATGATGAAGCCTCTCAGTGGTGGCCGAGAATTTATCTTTCCTAGCCGTATCAAGCCCAACCAACCAATGAATAGCCAAACAGTGAATGCAGCACTCAAGCGTGCTGGCTTAGGAGGTGTACTTGTTTCACACGGCTTGCGTTCTATCGCCAGTACGGCACTCAATGAGGAAGGATTTCC  >  NC_000913/2756736‑2757216                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                  cTGTTCCTAGTGTAAATATCGGAAAAGCTTTCGAGAAACCGCAAAAGAAAAACATGCCAAGCATCCGGCCGGATCAACTTCCGCAGCTAATGCACACCATGCGTACGGCAAGTATCAGCATGTCCACAAGATGCCTGTTCATGTGGCAACTTCTAACCATCACCCGCCCTGCCGAAGCTGCTGAGGCTCGATGGGATGAGATCGATTTCAATGCTAGCGAATGGAAAATTCCTGCAGCTCAAATGAAGATg                                                                                                                                                                                                                                         >  1:420985/1‑251 (MQ=255)       cTAGTGTAAATATCGGAAAAGCTTTCGAGAAACCGCAAAAGAAAAACATGCCAAGCATCCGGCCGGATCAACTTCCGCAGCTAATGCACACCATGCGTACGGCAAGTATCAGCATGTCCACAAGATGCCTGTTCATGTGGCAACTTCTAACCATCACCCGCCCTGCCGAAGCTGCTGAGGCTCGATGGGATGAGATCGATTTCAATGCTAGCGAATGGAAAATTCCTGCAGCCCAAATGAAGATGAACCgg                                                                                                                                                                                                                                   >  2:164773/1‑251 (MQ=255)         aGTGTAAATATCGGAAAAGCTTTCGAGAAACCGCAAAAGAAAAACATGCCAAGCATCCGGCCGGATCAACTTCCGCAGCTAATGCACACCATGCGTACGGCAAGTATCAGCATGTCCACAAGATGCCTGTTCATGTGGCAACTTCTAACCATCACCCGCCCTGCCGAAGCTGCTGAGGCTCGATGGGATGAGATCGATTTCAATGCTAGCGAATGGAAAATTCCTGCAGCTCAAATGAAGATGAACCgg                                                                                                                                                                                                                                   <  2:218301/249‑1 (MQ=255)            gtAAATATCGGAAAAGCTTTCTAGAAACCGCAAAAGAAAAACATGCCAAGCATCCGGCCGGATCAACTTCCGCAGCTAATGCACACCATGCGTACGGCAAGTATCAGCATGTCCACAAGATGCCTGTTCATGTGGCAACTTCTAACCATCACCCGCCCTGCCGAAGCTGCTGAGGCTCGATGGGATGAGATCGATTTCAATGCTAGCGAATGGAAAATTCCTGCAGCTCAAATGAAGATGAACCGGGAcc                                                                                                                                                                                                                               <  1:274029/250‑1 (MQ=255)                          aGCTTTCGAGAAAACGCGAAAGAAAAAAATGCCAAGCATCCGGCCGGATCAACATCCGCAGCTAATGCACACCAGGCGTACGGCAAGAATCAGCATGTCCACAAGATGCCTGTTCAAGTGGCAACTTCTAACCATCACCCGCCCTGCCGAAGCTGCTGAGGCTCGATGGGGTGAGCTCGACTTCAATGCTAGCGAATGGAAAATTCCTGACGCTCAACTGAAGATGCACCGTGACCATACGGTTACACttt                                                                                                                                                                                                                >  2:467986/1‑249 (MQ=255)                                cGAGAAACCGCAAAAGAAAAACATGCCAAGCATCCGGCCGGATCAACTTCCGCAGCTAATGCACACCATGCGTACGGCAAGTATCAGCATGTCCACAAGATGCCTGTTCATGTGGCAACTTCTAACCATCACCCGCCCTGCCGAAGCTGCTGAGGCTCGATGGGATGAGATCGATTTCAATGCTAGCGAATGGAAAATTCCTGCAGCTCAAATGAAGATGAACCGGGACCATACGGTTCCACT‑ATCTGATg                                                                                                                                                                                                         <  1:213736/251‑1 (MQ=255)                                  agaAACCGCCAAAGAACAACATGCCAAGCATCCGGCCGGCTCAACTTACGCAGCGAATGCACACCATGCGTACGGCAAGTATCAGCATGTCCTCAAGATGCCTGATCATGTGGCAACTTCTAACCATCACCAGCCCTGCCGACGCTGATGAGGGTCTATGGGGTGGGATCGGTTTCAATGCTAGCGGATGGAAAAGTCCTGCAGCTCAAATGAAGATGAACCGGGAccccaaggatccactagatgagggg                                                                                                                                                                                                        >  2:454564/1‑228 (MQ=255)                                                aaaaaCATGCCAAGCATCCGGCCGGATCAACTTCCGCAGCTAATGCACACCATGCGTACGGCAAGTATCAGCATGTCCACAAGATGCCTGTTCATGTGGCAACTTCTAACCATCACCCGCCCTGCCGAAGCTGCTGAGGCTCGATGGGATGAGATCGATTTCAATGCTAGCGAATGGAAAATTCCTGCAGCTCAAATGAAGATGAACCGGGACCATACGGTTCCACT‑ATCTGATGGGGCTCTTGCTATTCt                                                                                                                                                                                         <  1:1402/251‑1 (MQ=255)                                                        gCCAAGCATCCGGCCGGATCAACTTCCGCAGCTAATGCACACCATGCGTACGGCAAGTATCAGCATGTCCACAAGATGCCTGTTCATGTGGCAACTTCTAACCATCACCCGCCCTGCCGAAGCTGCTGAGGCTCGATGGGATGAGATCGATTTCAATGCTAGCGAATGGAAAATTCCTGCAGCTCAAATGAAGATGAACCGGGACCATACGGTTCCACT‑ATCTGATGGGGCTCTTGCTATTCTGGAAatg                                                                                                                                                                                  >  1:137765/1‑250 (MQ=255)                                                         ccAAGCATCCGGCCGGATCAACTTCCGCAGCTAATGCACACCATGCGTACGGCAAGTATCAGCATGTCCACAAGATGCCTGTTCATGTGGCAACTTCTAACCATCACCCGCCCTGCCGAAGCTGCTGAGGCTCGATGGGATGAGATCGATTTCAATGCTAGCGAATGGAAAATTCCTGCAGCTCAAATGAAGATGAACCGGGACCATACGGTTCCACT‑ATCTGATGGGGCTCTTGCTATTCTGGAAatgat                                                                                                                                                                                >  1:43785/1‑251 (MQ=255)                                                          cAAGCATCCGGCCGGATCAACTTCCGCAGCTAATGCACACCATGCGTACGGCAAGTATCAGCATGTCAACACGATGCCTGTTCATGTGGCAACTTCTAACCATCACCCGCCCTGCCGAAGCTGCTGAGGCTCGCTGGGATGAGATCGATTTCAATGCTAGCGAATGGAAAATTCCTGCAGCTCAAATGAAGATGAACCGGGAACATACGGTTCCCCT‑ATCTGATGGGGCTCTTGCTATTCTGGAAatgatt                                                                                                                                                                               >  2:185919/1‑250 (MQ=255)                                                                    gccggATCAACCTCCGCATCTAATTCATACCATGCGTACGGCATGTATAAGCATGTCCACAAGATTCCGGTTCATGTGGCATCTTTGAACCATCACCCGCCCTGCCGAAGCGGCTGAGGCTCGATGGGATGAGATCGATTTCAATGCTAGCGAATGGAAAATTCCTGCAGCTCAAATGAAGATGAACCGGGACCATACGGTTCCACT‑ATCTGATGGGGCTCTTGCTATTCTGGAAATGATGAAGCCTCTCa                                                                                                                                                                     <  2:252697/251‑1 (MQ=255)                                                                       ggATCAACTTCCGCAGCTAATGCACACCATGCGTACGGCAAGTATCAGCATGTCCACAAGATGCCTGTTCATGTGGCAACTTCTAACCATCACCCGCCCTGCCGAAGCTGCTGAGGCTCGATGGGATGAGATCGATTTCAATGCTAGCGAATGGAAAATTCCTGCAGCTCAAATGAAGATGAACCGGGACCATACGGTTCCACT‑ATCTGATGGGGCTCTTGCTATTCTGGAAATGATGAAGCCTCTCAgtg                                                                                                                                                                  >  1:152698/1‑251 (MQ=255)                                                                           cAACTTCCGCAGCTAATGCACACCATGCGTACGGCAAGTATCAGCATGTCCACAAGATGCCTGTTCATGTGGCAACTTCTAACCATCACCCGCCCTGCCGAAGCTGCTGAGGCTCGATGGGATGAGATCGATTTCAATGCTAGCGAATGGAAAATTCCTGCAGCTCAAATGAAGATGAACCGGGACCATACGGTTCCACT‑ATCTGTTGGGGCTCTTGCTATTCTGGAAATGATGAAGCCTCTCAGtggtgg                                                                                                                                                              >  2:357218/1‑251 (MQ=255)                                                                                   gCAGCTAATGCACACCATGCGTACGGCAAGTATCAGCATGTCCACAAGATGCCTGTTCATGTGGCAACTTCTAACCATCACCCGCCCTGCCGAAGCTGCTGAGGCTCGATGGGATGAGATCGATTTCAATGCTAGCGAATGGAAAATTCCTGCAGCTCAAATGAAGATGAACCGGGACCATACGGTTCCACT‑ATCTGATGGGGCTCTTGCTATTCTGGAAATGATGAAGCCTCTCAGTGGTGGCCGAGAAt                                                                                                                                                      >  1:353470/1‑251 (MQ=255)                                                                                    cAGCTAATGCACACCATGCGTACGGCAAGTATCAGCATGTCCACAAGATGCCTGTTCATGTGGCAACTTCTAACCATCACCCGCCCTGCCGAAGCTGCTGAGGCTCGATGGGATGAGATCGATTTCAATGCTAGCGAATGGAAAATTCCTGCAGCTCAAATGAAGATGAACCGGGACCATACGGTTCCACT‑ATCTGATGGGGCTCTTGCTATTCTGGAAATGATGAAGCCTCTCCGTGGTGGCCGAGAAtt                                                                                                                                                     >  2:149857/1‑251 (MQ=255)                                                                                                   attcGTACGGCAAGTATCAGCATGTCCACAAGATGCCTGTTCATGTGGCAACTTCTAACCATCACCCGCCCTGCCGAAGCTGCTGAGGCTCGATGGGATGAGATCGATTTCAATGCTAGCGAATGGAAAATTCCTGCAGCTCAAATGAAGATGAACCGGGACCATACGGTTCCACT‑ATCTGATGGGGCTCTTGCTATTCTGGAAATGATGAAGCCTCTCAGTGGTGGCCGAGAATTTATCTTTCCTAGCCg                                                                                                                                      >  1:48066/4‑251 (MQ=255)                                                                                                     gCGTACGGCAAGTATCAGCATGTCCACAAGATGCCTGTTCATGTGGCAACTTCTAACCATCACCCGCCCTGCCGAAGCTGCTGAGGCTCGATGGGATGAGATCGATTTCAATGCTAGCGAATGGAAAATTCCTGCAGCTCAAATGAAGATGAACCGGGACCATACGGTTCCACT‑ATCTGATGGGGCTCTTGCTATTCTGGAAATGATGAAGCCTCTCAGTGGTGGCCGAGAATTTATCTTTCCTAGCCGTa                                                                                                                                    <  1:104688/251‑1 (MQ=255)                                                                                                                       cATGTCCACAAGATGCCTGTTCATGTGGCAACTTCTAACCATCACCCGCCCTGCCGAAGCTGCTGAGGCTCGATGGGATGAGATCGATTTCAATGCTAGCGAATGGAAAATTCCTGCAGCTCAAATGAAGATGAACCGGGACCATACGGTTCCACT‑ATCTGATGGGGCTCTTGCTATTCTGGAAATGATGAAGCCTCTCAGTGGTGGCCGAGAATTTATCTTTCCTAGCCGTATCAAGCccaaccaacca                                                                                                                   >  1:74918/1‑250 (MQ=255)                                                                                                                                  gATGCCTGTTCATGTGGCAACTTCTAACCATCACCCGCCCTGCCGAAGCTGCTGAGGCTCGATGGGATGAGATCGATTTCAATGCTAGCGAATGGAAAATTCCTGCAGCTCAAATGAAGATGAACCGTGACCATACGGTTCCACT‑ATCTGATGGGGCTCTTGCTATTCTGGAAATGATGAAGCCTCTCAGTGGTGGCCGAGAATTTATCTTTCCTAGCCGTATCAAGCCCAACCAACCAATGAATAGCCaa                                                                                                       <  1:210878/251‑1 (MQ=255)                                                                                                                                     gCCTGTTCATGTGGCAACTTCTAACCATCACCCGCCCTGCCGAAGCTGCTGAGGCTCGATGGGATGAGATCGATTTCAATGCTAGCGAATGGAAAATTCCTGCAGCTCAAATGAAGATGAACCGGGACCATACGGTTCCACT‑ATCTGATGGGGCTCTTGCTATTCTGGAAATGATGAAGCCTCTCAGTGGTGGCCGAGAATTTATCTTTCCTAGCCGTATCAAGCCCAAACAACCAATGAATAGCCAAAc                                                                                                     >  2:216472/1‑250 (MQ=255)                                                                                                                                            cATGTGGCAACTTCTAACCATCACCCGCCCTGCCGAAGCTGCTGAGGCTCGATGGGATGAGATCGATTTCAATGCTAGCGAATGGAAAATTCCTGCAGCTCAAATGAAGATGAACCGGGACCATACGGTTCCACT‑ATCTGATGGGGCTCTTGCTATTCTGGAAATGATGAAGCCTCTCAGTGGTGGCCGAGAATTTATCTTTCCt                                                                                                                                           >  2:185452/1‑205 (MQ=255)                                                                                                                                            cATGTGGCAACTTCTAACCATCACCCGCCCTGCCGAAGCTGCTGAGGCTCGATGGGATGAGATCGATTTCAATGCTAGCGAATGGAAAATTCCTGCAGCTCAAATGAAGATGAACCGGGACCATACGGTTCCACT‑ATCTGATGGGGCTCTTGCTATTCTGGAAATGATGAAGCCTCTCAGTGGTGGCCGAGAATTTATCTTTCCt                                                                                                                                           <  1:185452/205‑1 (MQ=255)                                                                                                                                              tgtgGCAACTTCTAACCATCACCCGCCCTGCCGAAGCTGCTGAGGCTCGATGGGATGAGATCGATTTCAATGCTAGCGAATGGAAAATTCCTGCAGCTCAAATGAAGATGAACCGGGACCATACGGTTCCACT‑ATCTGATgggg                                                                                                                                                                                                      <  1:37741/144‑1 (MQ=255)                                                                                                                                              tgtgGCAACTTCTAACCATCACCCGCCCTGCCGAAGCTGCTGAGGCTCGATGGGATGAGATCGATTTCAATGCTAGCGAATGGAAAATTCCTGCAGCTCAAATGAAGATGAACCGGGACCATACGGTTCCACT‑ATCTGATgggg                                                                                                                                                                                                      >  2:37741/1‑144 (MQ=255)                                                                                                                                                             ccATCACCCGCCCTGCCGAAGCTGCTGAGGCTCGATGGGATGAGATCGATTTCAATGCTAGCGAATGGAAAATTCCTGCAGCTCAAATGAAGATGAACCGGGACCATACGGTTCCACT‑ATCTGATGGGGCTCTTGCTATTCTGGAAATGATGAAGCCTCTCAGTGGTGGCCGAGAATTTATCTTTCCTAGCCGTATCAAGCCCAACCAACCAATGAATAGCCAAACAGTGAATGCAGCACTCAAGCGTGCt                                                                            >  1:182040/1‑251 (MQ=255)                                                                                                                                                                ccccccGCCCTGCCGAAGCTGCTGAGGCTAGATGGGATGAGATCGATTTCAATGCTAGCGAATGGAAAATTCCTGCAGCTCAAATGAAGATGAACCGGGACCATACGGTTCCACT‑ATCTGATGGGGCTCTTGCTATTCTGGAAATGATGAAGCCTCTCAGTGGTGGCCGAGAATTTATCTTTCCTAGCCGTATCAAGCCCAACCAACCAATGAATAGCCAAACAGTGAATGCAGCACTCAAGCGTGCTGGc                                                                         <  1:305275/248‑1 (MQ=255)                                                                                                                                                                    ccGCCCTGCCGAAGCTGCTGAGGCTCGATGGGATGAGATCGATTTCAATGCTAGCGAATGGAAAATTCCTGCAGCTCAAATGAAGATGAACCGGGACCATACGGTTCCACT‑ATCTGATGGGGCTCTTGCTATTCTGGAAATGATGAAGCCTCTCAGTGGGGGCCGAGAATTTATCTTTCCTAGCCGTATCAAGCCCAACCAACCAATGAATAGCCAAACAGTGAATGCAGCACTCAAGCGTGCTGGCtt                                                                       >  1:185961/1‑249 (MQ=255)                                                                                                                                                                     cGCCCTGCCGAAGCTGCTGAGGCTCGATGGGATGAGATCGATTTCAATGCTAGCGAATGGAAAATTCCTGCAGCTCAAATGAAGATGAACCGGGACCATACGGTTCCACT‑ATCTGATGGGGCTCTTGCTATTCTGGAAATGATGAAGCCTCTCAGTGGTGGCCGAGAATTTATCTTTCCTAGCCGTATCAAGCCCAACCAACCAATGAATAGCCAAACAGTGAATGCAGCACTCAAGCGTGCTGGCTTagg                                                                    >  1:15501/1‑251 (MQ=255)                                                                                                                                                                                    gcgggggCTCGAGGGGATGAGATCGATTTAAATGCTAGCGAATGGAAAATTCCTGCAGCGCAAATGAAGATGAACCGGGACCATACGGTTCCACT‑ATCTGATGGGGCTCTTGCTATTCTGGAAATGATGAAGCCTCTCAGTGGTGGCCGAGAATTTATCTTTCCTAGCCGTATCAAGCCCAACCAACCAATGAATAGCCAAACAGTGAATGCAGCACTCAAGCGTGCTGGCTTAGGAGGTGTACTTGTTTc                                                     <  2:415774/246‑1 (MQ=255)                                                                                                                                                                                      tgAGGCTCGATGGGATGAGATCGATTTCAATGCTAGCGAATGGAAAATTCCTGCAGCTCAAATGAAGATGAACCGGGACCATACGGTTCCACT‑ATCTGATGGGGCTCTTGCTATTCTGGAAATGATGAAGCCTCTCAGTGGTGGCCGAGAATTTATCTTTCCTAGCCGTATCAAGCCCAACCAACCAATGAATAGCCAAACAGTGAATGCAGCACTAAAGCGTGCTGGCTTAGGAGGTGTACTTGTTTcac                                                   >  1:428023/1‑251 (MQ=255)                                                                                                                                                                                       gAGGCTCGATGGGATGAGATCGATTTCAATGCTAGCGAATGGAAAATTCCTGCAGCTCAAATGAAGATGAACCGGGACCATACGGTTCCACT‑ATCTGATGGGGCTCTTGCTATGCTGGAAATGATGAAGCCTCTCAGTGGTGGCCGAGAATTTATCTTTCCTAGCCGTATCAAGCCCAACCAACCAATGAATAGCCAAACATTGAATGCAGCACTCAAGCGTGCTGGCTTAGGAGGTGTACTTGTTTcaca                                                  <  1:400104/251‑1 (MQ=255)                                                                                                                                                                                            tCGATGGGATGAGATCGATTTCAATGCTAGCGAATGGAAAATTCCTGCAGCTCAAATGAAGATGAACCGGGACCATACGGTTCCACT‑ATCTGATGGGGCTCTTGCTATTCTGGAAATGATGAAGCCTCTCAGTGGTGGCCGAGAATTTATCTTTCCTAGCCGTATCAAGCCCAACCAACCAATGAATAGCCAAACCGTGAATGCAGCACTCAAGCGAGCTGGCTTAGGAGGAGTACTTGTTTCACACGGCt                                             >  2:464610/1‑251 (MQ=255)                                                                                                                                                                                               aTGGGATGAGATCGATTTCAATGCTAGCGAATGGAAAATTCCTGCAGCTCAAATGAAGATGAACCGGGACCATACGGTTCCACT‑ATCTGATGGGGCTCTTGCTATTCTGGAAATGATGAAGCCTCTCAGTGGTGGCCGAGAATTTATCTTTCCTAGCCGTATCAAGCCCAACCAACCAATGAATAGCCAAACAGTGAATGCAGCACTCAAGCGTGCTGGCTTAGGAGGTGTACTTGTTTCACACGGCTTGc                                          <  1:415123/251‑1 (MQ=255)                                                                                                                                                                                               aTGGGATGAGATCGATTTCAATGCTAGCGAATGGAAAATTCCTGCAGCTCAAATGAAGATGAACCGGGACCATACGGTTCCACT‑ATCTGATGGGGCTCTTGCTATTCTGGAAATGATGAAGCCTCTCAGTGGTGGCCGAGAATTTATCTTTCCTAGCCGTATCAAGCCCAACCAACCAATGAATAGCCAAACAGTGAATGCAGCACTCAAGCGTGCTGGCTTAGGAGGTGTACTTGTTTCACACGGCTTGc                                          >  2:111674/1‑251 (MQ=255)                                                                                                                                                                                                      gagaTCGGTTTCAATGCTAGCGAATGGAAAATTCCTGCAGCTCAAATGAAGATGAACCGGGACCATAAGGTTCCACT‑ATCTGATGGGGCTCTTGCTATTCTGGAAATGATGAAGCCTATCAGAGGTGGCCGAGAATTTATCTTTCCTAGCCGTATCAAGCCCACCCAACCAATGAATACACAAACTGTGAATGCAGCACTCAATCGTTCTGGCTTAGGAGGTGAACTTGTTTCACCAGGCTTGCGTTCTAt                                   >  2:239635/1‑251 (MQ=255)                                                                                                                                                                                                       agaTCGATTTCAATGCTAGCGAATGGAAAATTCCTGCAGCTCAAATGAAGATGAACCGGGACCATACGGTTCCACT‑ATCTGATGGGGCTCTTGCTATTCTGGAAATGATGAAGCCTCTCAGTGGTGGCCGAGAATTTATCTTTCCTAGCCGTATCAAGCCCAACCAACCAATGAATAGCCAAACAGTGAATGCAGCACTCAAGCGTGCTGGCTTAGGAGGTGTACTTGTTTCACACGGCTTGCGTTCTATc                                  >  1:340340/1‑251 (MQ=255)                                                                                                                                                                                                               ttCAATGCTAGCGAATGGAAAATTCCTGCAGCTCAAATGAAGATGAACCGGGACCATACGGTTCCACT‑ATCTGATGGGGCTCTTGCTATTCTGGAAATGATGAAGCCTCTCAGTGGTGGCCGAGAATTTATCTTTCCTAGCCGTATCAAGCCCAACCAACCAATGAATAGCCAAACAGTGAATGCAGCACTCAAGCGTGCTGGCTTAGGAGGTGTACTTGTTTCACACGGCTTGCGTTCTATCGCCAGTAc                          >  1:469295/1‑251 (MQ=255)                                                                                                                                                                                                                     gcgaGCGAATGTAAAATTCCTGCAGCTCAAATGAAGATGACCCGGGACAATCCGGTTCAACA‑ATCTGAGGGGGCTCTTGCTATCCTTGAAATGATGAAGCCTCTCATTGGGGGCCGAGAATTTACCTTCCCTAGCCGTATCAAGCCCAACCAACCAATGAATAGCCAAACAGGGAATGCAGCACTCAAGCGTGCTGGCCTAGGAGGTGTACTTGTTTCACCCGGCTTGCGTTCTATCGCCAGTACGGCACt                    <  2:46217/248‑1 (MQ=255)                                                                                                                                                                                                                     gcgaGCGAAGGAAAAATTCCTGCAGCTCAAATGAAGATGAACCGGAACCAT‑CCGTTTCACTTATCGGATGGGGTTCTTGCTATTCTGGAAATGATGAAGCCTCTCAGTGGTGGCCGTGAATTTAGCTTTCCTAGCCGTATCAAGCCCAACCAACCAATGAATAGCCAAACAGTGAATGCAGCACTCAAGCGTGCTGGCTTAGGAGGTGTACTTGTTTCACACGGTTTGCGTTCTATCGCCAGTACGGTACt                    <  2:292184/248‑1 (MQ=255)                                                                                                                                                                                                                            aaTGGAAAATTCCTGCAGCTCAAATGAAGATGAACCGGGACCATACGGTTCCACT‑ATCTGATGGGGCTCTTGCTATTCTGGAAATGATGAAGCCTCTCAGTGGTGGCCGAGAATTTATCTTTCCTAGCCGTATCAATCCCAACCAACCAATGAATAGCCAAACAGTGAATGAAGCACTCAAGCGTGCTGGCTTCGGGGGTGTACTTTTTTCACACGGCTTGCGTTCTATCGCCAGTACGGCACtatatgtg             >  2:204990/1‑244 (MQ=255)                                                                                                                                                                                                                                  aaaTTCCTGCAGCTCAAATGAAGCTGAACCGGGACCATACGGTTCCCCT‑ATCTGATGGGGCTCTTGCTATTCTGGCACTGATGAAGCCTCTCAGTGGTGGCCGAGAATTTATCTTTCATAGCCGTATCAAGACCAACCAACCAATGAATAGCCACACAGTGAATGCAGCACTCAAGCGTGCTGGCTTAGGAGGTGTAATTGTTTCACACGGCTTGCGTTCTATCGCCAGTCTGGCACTCACTGaggaagg        >  2:205960/1‑250 (MQ=255)                                                                                                                                                                                                                                  aaaTTCCTGCAGCTCAAATGAAGATGAACCGGGACCATACGGTTCCACT‑ATCTGATGGGGCTCTTGCTATTCTGGAAATGATGaa                                                                                                                                                                             <  2:131833/85‑1 (MQ=255)                                                                                                                                                                                                                                  aaaTTCCTGCAGCTCAAATGAAGATGAACCGGGACCATACGGTTCCACT‑ATCTGATGGGGCTCTTGCTATTCTGGAAATGATGaa                                                                                                                                                                             >  1:131833/1‑85 (MQ=255)                                                                                                                                                                                                                                   aaTTCCTGCAGCTCAAATGAAGATGAACCGGGACCATACGGTTCCACT‑ATCTGATGGGGCTCTTGCTATTCTGGAAATGATGAAGCCTCTCAGTGGTGGCCGAGAATTTATCTTTCCTAGCCGTATCAAGCCCAACCAACCAATGAATAGCCAAACAGTGAATGCAGCACTCAAGCGTGCTGGCTTAGGAGGTGTACTTGTTTCACACGGCTTGCGTTCTATCGCCAGTAAGGCGCTCCATGAGGAAGGAt      >  2:392952/1‑251 (MQ=255)                                                                                                                                                                                                                                       ccTGCAGCTCAAATGAAGATGAACCGGGACCATACGGTTCCACT‑ATCTGATGGGGCTCTTGCTATTCTGGAAATGATGAAGCCTCTCAGTGGTGGCCGAGAATTTATCTTTCCTAGCCGTATCAAGCCCAACCAACCAATGAATAGCCAAACAGTGAATGCAGCACTCAAGCGTGCTGGCTTAGGAGGTGTACTTGTTTCACACGGCTTGCGTTCTATCGCCAGTACGGCACTCAATGAGGAAGGGTTTcc  >  2:230003/1‑251 (MQ=255)                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                  CTGTTCCTAGTGTAAATATCGGAAAAGCTTTCGAGAAACCGCAAAAGAAAAACATGCCAAGCATCCGGCCGGATCAACTTCCGCAGCTAATGCACACCATGCGTACGGCAAGTATCAGCATGTCCACAAGATGCCTGTTCATGTGGCAACTTCTAACCATCACCCGCCCTGCCGAAGCTGCTGAGGCTCGATGGGATGAGATCGATTTCAATGCTAGCGAATGGAAAATTCCTGCAGCTCGAATGAAGATGAACCGGGACCATACGGTTCCACT‑ATCTGATGGGGCTCTTGCTATTCTGGAAATGATGAAGCCTCTCAGTGGTGGCCGAGAATTTATCTTTCCTAGCCGTATCAAGCCCAACCAACCAATGAATAGCCAAACAGTGAATGCAGCACTCAAGCGTGCTGGCTTAGGAGGTGTACTTGTTTCACACGGCTTGCGTTCTATCGCCAGTACGGCACTCAATGAGGAAGGATTTCC  >  NC_000913/2756736‑2757216

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑