Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 84,267 G→C intergenic (‑559/‑101) leuL ← / → leuO leu operon leader peptide/DNA‑binding transcriptional dual regulator LeuO

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00091384,2670GC96.2% 77.0 / NA 27intergenic (‑559/‑101)leuL/leuOleu operon leader peptide/DNA‑binding transcriptional dual regulator LeuO
Reads supporting (aligned to +/- strand):  ref base G (1/0);  major base C (16/9);  minor base A (1/0);  total (18/9)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.26e-01

TTTGGTGAATATTATTGATCAATTAATGTTAAGAATTAATGCATTAAATATATAAATTAATTATTAAATAAGCACATTTAATCCATTTTGTAGATGATTGAGTATTCGCGGTAGTTATGATTAGATTGTTTTCGCAACAAAAACATTATGGATTATTATGCTGTGGTAAATGACTCATTCCACGGCAATGGATTCTGTTTTTATCAGAACCCGTATCTTTATGTTTTCCGAATTTTACTCATTTTGCTTTTTCTTATTTTATATGCATGATAAATCATATTCTTCAGGATTATTTCTCTGCATTCCAATAAGGGAAAGGGAGTTAAGTGTGACAGTGGAGTTAAGTATGCCAGAGGTACAAACAGATCATCCAGAGACGGCGGAGTTAAGCAAACCACAGCTACGCATGGTCGATCTCAACTTATTAACCGTTTTCGATGCCGTGATGCAGGAGCAAAACATTACTCGTGCCGCTCATGTTCTGGGAAT  >  NC_000913/84022‑84510
                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                   
tttGGTGAATATTATTGATCAATTAATGTTAAGAATTAATGCATTAAATATATAAATTAATTATTAAATAAGCACATTTAATCCATTTTGTAGATGATTGAGTATTCGCGGTAGTTATGATTAGATTGTTTTCGCAACAAAAACATTATGGATTATTATGCTGTGGTAAATGACTCATTCCACGGCAATGGATTCTGTTTTTATCAGAACCCGTATCTTTATGTTTTCCTAATTTTACTCATTTTCCtttt                                                                                                                                                                                                                                                >  1:17692/1‑251 (MQ=255)
   ggTGAATATTATTGATCAATTAATGTTAAGAATTAATGCATTAAATATATAAATTAATTATTAAATAAGCACATTTAATCCATTTTGTAGATGATTGAGTATTCGCGGTAGTTATGATTAGATTGTTTTCGCAACAAAAACATTATGGATTATTATGCTGTGGTAAATGACTCATTCCACGGCAATGGATTCTGTTTTTATCAGAACCCGTATCTTTATGTTTTCCTAATTTTACTCATTTTCCTTTTTCt                                                                                                                                                                                                                                             <  2:443637/251‑1 (MQ=255)
                 aTCAATTAATGTTAAGAATTAATGCATTAAATATATAAATTAATTATTAAATAAGCACATTTAATCCATTTTGTAGATGATTGAGTATTCGCGGTAGTTATGATTAGATTGTTTTCGCAACAAAAACATTATGGATTATTATGCTGTGGTAAATGACTCATTCCACGGCAATGGATTCTGTTTTTATCAGAACCCGTATCTTTATGTTTTCCTAATTTTACTCATTTTCCTTTTTCTTATTTTATATGCAt                                                                                                                                                                                                                               <  1:398714/251‑1 (MQ=255)
                            ttAAGAATTAATGCATTAAATATATAAATTAATTCTTAAATAAGCACATTTAATCCATTTTGTAGTTGATTGAGTATTCGCGGTAGTTATGATTAGATTGTTTTCGCAACAAAAACATTATGGATTATTATGCTGTGGTAAATGACTCATTCAACGGCAATGGATTATGTTTTTATCAGAACCCGTATATTTATGTTTTCCTAATTTTACTCATTTTCCTTTTGCTTATTTTATATGCATGTTAAATCatt                                                                                                                                                                                                                    >  2:325724/1‑250 (MQ=255)
                            ttAAGAATTAATGCATTAAATATATAAATTAATTATTAAATAAGCAAATTTAATCCATTTTGTAGATGATTGAGTATTCGAGGTAGTATTGATTAGATTGTTTTCGCAATAAAAACATTATGGATTATTATGCTGTGGTAAATGACTCATTCAACGGCAATGGATTCTGTTTTTATCAGAACCCGTATCTTTATGTTTTCCTAATTTTACTCATTTTCCTTTTTCTTATTTTATATGCATGTTAAATCat                                                                                                                                                                                                                     >  2:38513/1‑250 (MQ=255)
                                                                 aaaTAAGCACATTTAAGTCATTGTGTAGATGATTGAGTATTCGCGGTAGTTATGATTATATTGTTTTCGCAACACAAACATTATGTATTATTATGCTTTGGTAAATGACTCATTCCACGGTAATGGATTCTGTTTTTATCAGAACCCGTATCTTTATGTTTTCCTAATTTTACGCATGTTCCTTTGTCTTATTTTATATGCATGATAAATCATATTCTCGAGGATTATTACTCTGCATTCCAATaagtgat                                                                                                                                                                               <  2:435853/251‑5 (MQ=255)
                                                                  aaTAAGCACATTTAATCCATTTTGTAGATGATTGAGTATTCGCGGTAGTTATGATTAGATTGTTTTCGCAACAAAAACATTATGGATTATTATGCTGTGGTAAATGACTCATTCCACGGCAATGGATTCTGTTTTTATCAGAACCCGTATCTTTATGTTTTCCTAATTTTACTCATTTTCCTTTTTCTTATTTTATATGCATGATAAATCATATTCTCGAGGATTATTTCTCTGCATTCCAATaagggaa                                                                                                                                                                               >  1:275104/1‑250 (MQ=255)
                                                                  aaTAAGCAAATTTACTCAATTTTGTAGGTGATTGAGTATTCGCGGAAGTTATGATTAGATTGTTTTCGCAACAAAAACATTATGGATTATTATGCTGTGGTCAATGGCTCATTCCACGACAATGACTTCTGTTTTTATCAGAACCCGGAGCTTTATATTTTCCTAATTTTACTCATTTTACGTTTTCATATTTTAGATGCATAATAAATCTTATACTCGAGGATTATTTCTATGCATTCCACTaagggaa                                                                                                                                                                               >  2:136496/1‑250 (MQ=255)
                                                                                      tttGTAGATGATTGAGTATTCGCGGTAGTTATGATTAGATTGTTTTCGCAACAAAAACATTATGGATTATTATGCTGTGGTAAATGACTCATTCCACGGCAATGGATTCTGTTTTTATCAGAACCCGTATCTTTATGTTTTCCTAATTTTACTCATTTTCCTTTTTCTTATTTTATATGCATGATAAATCATATTCTCGAGTATTATTTCTCTGCATTCCATTAAGGGAAAGGGTGTTAAGTGTGACAGTg                                                                                                                                                          >  2:18207/1‑251 (MQ=255)
                                                                                          tAGATGATTGAGTATTAGCGGTAGTTATGATTAGATTGTTTTCGCAACAAAAACATTATGGATTATTATGCTGTGGTAAATGACTCATTCCACGGAAATGGATTCTGTTTTTATCAGAACCCGTATCTTTATGTTTTCCTAATTTTACTCATTTTCCTTTTTCTTATTTTATATGCATGATAAATCATATTCTCGAGGATTATTTCTCTGCATTCCAATAAGGGAAAGGGAGTTAAGTGTGACAGTGGAGt                                                                                                                                                      >  2:182726/1‑251 (MQ=255)
                                                                                                                                                      gATTATTATGCTGTGGTAAATGACTCATTCCACGGCAATGGATTCTGTTTTTATCAGAACCCGTATCTT‑ATGTTTTCCGAATTTTACTCATTTTGCTTTTTCTTATTTTATATGCATGATTAATCATATTCTTCAGGATTATTTCTCTGCATTCCAATAAGGGAAAGGGAGTTAAGTGTGACAGTGGAGTTAAGTATGCCAGAGGTACAAACAGATCATCCAGAGACGGCGGAGTTAAGCAAACCACAGCt                                                                                         >  1:89813/1‑251 (MQ=255)
                                                                                                                                                          attaaGCTGTTGTAAATGACTCATTCCACGGCAATTGATTCTGTTTTTATCAGAACCCGTATCTTTATGTTTTCCTAATTTTACTCATTTTCCTTTTTCTTATTTTATATGCATGATAAACCATATTCTCGAGGATTATTTCTCTGCATTCCAATAAGGGAAAGGGAGTTAAGTGTGACAGTGGAGTTAAGTATGCCAGAGGTACAAACAGATCATCCAGAGACGGCGGAGTTAAGCAAGCCACAGCTAc                                                                                       <  2:172288/250‑1 (MQ=255)
                                                                                                                                                            tatGCTGTGGTAAATGACTCATTCCACGGCAATGGATTCTGTTTTTATCAGAAACCGTATCTTTATGTTTTCCTAATTTTACTCATTTTCCTTTTTCTTATTTTATATGCATGATAAATCATATTCTCGAGGATTATTTCTCTGCATTCCAATAAGGGAAAGGGAGTTAAGTGTGACAGTGGAGTTAAGTATGCCAGAGGTACAAACAGATCATCCAGAGACGGCGGAGTTAAGCAAGCCACAGCTACGc                                                                                     >  1:240277/1‑250 (MQ=255)
                                                                                                                                                             atGCTGTGGTAAATGACTCATTCCACGGCAATGGATTCTGTTTTTATCAGAACCCGTATCTTTATGTTTTCCTAATTTTACTCATTTTCCTTTTTCTTATTTTATATGCATGATAAATCATATTCTCGAGGATTATTTCTCTGCATTCCAATaagggaa                                                                                                                                                                               <  1:329010/159‑1 (MQ=255)
                                                                                                                                                             atGCTGTGGTAAATGACTCATTCCACGGCAATGGATTCTGTTTTTATCAGAACCCGTATCTTTATGTTTTCCTAATTTTACTCATTTTCCTTTTTCTTATTTTATATGCATGATAAATCATATTCTCGAGGATTATTTCTCTGCATTCCAATaagggaa                                                                                                                                                                               >  2:329010/1‑159 (MQ=255)
                                                                                                                                                               gCTGTGGTAAATGACTCATTCCACGGCAATGGATTCTGTTTTTATCAGAACCCGTATCTTTATGTTTTCCTAATTTTACTCATTTTCCTTTTTCTTATTTTATATGCATGATAAATCATATTCTCGAGGATTATTTCTCTGCATTCCAATAAGGGAAAGGGAGTTAAGTGTGACAGTGGAGTTAAGTATGCCAGAGGTACAAACAGATCATCCAGAGACGGCGGAGTTAAGCAAGCCACAGCTACGCATgg                                                                                 >  1:115746/1‑251 (MQ=255)
                                                                                                                                                                             cTCATTCCACGGCAATGGATTCTGTTTTTATCAGAACCCGTATCTTTATGTTTTCCTAATTTTACTCATTTTCCTTTTTCTTATTTTATATGCATGATAAATCATATTCTCGAGGATTATTTCTCTGCATTCCAATAAGGGAAAGGGAGTTAAGTGTGACAGTGGAGTTAAGTATGCCAGAGGTACAAACAGATCATCCAGAGACGGCGGAGTTAAGCAAGCCACAGCTACGCATGGTCGATCTCAACtt                                                                    >  1:266369/1‑250 (MQ=255)
                                                                                                                                                                                  tCCACGGCAATGGATTCTGTTTTTATCAGAACCCGTATCTTTATGTTTTCCTAATTTTACTCATTTTCCTTTTTCTTATTTTATATGCATGATAAATCATATTCTCGAGGATTATTTCTCTGCATTCCAATAAGGGAAAGGGAGTTAAGTGTGACAGTGGAGTTAAGTATGCCAGAGGTACAAACAGATCATCCAGAGAcgg                                                                                                               >  2:333649/1‑202 (MQ=255)
                                                                                                                                                                                  tCCACGGCAATGGATTCTGTTTTTATCAGAACCCGTATCTTTATGTTTTCCTAATTTTACTCATTTTCCTTTTTCTTATTTTATATGCATGATAAATCATATTCTCGAGGATTATTTCTCTGCATTCCAATAAGGGAAAGGGAGTTAAGTGTGACAGTGGAGTTAAGTATGCCAGAGGTACAAACAGATCATCCAGAGAcgg                                                                                                               <  1:333649/202‑1 (MQ=255)
                                                                                                                                                                                                 ttggTTTTTACCAGAACCCGGTTCTTTATTTTTTCCTAATTTTCCTCATTTTCCTTTTTCTTATTTTATATGCATGATAAATCATATTCGCGAGGATTATTTCTCTGCATTCCAATAAGGGAAAGGGAGTTAAGTGTGACAGTGGAGTTAAGTATGCCAGAGTTACAAACAGATCATCCAGAGACGGCGGAGTTAAGCAAGCCACAGCTACGCATGGTCGATCTCAACTTATTAACCGTTTTCGATgtcg                                                <  2:249260/247‑4 (MQ=255)
                                                                                                                                                                                                    gTTTTTATCAGAACCCGTATCTTTATGTTTTCCTAATTTTACTCATTTTCCTTTTTCTTATTTTATATGCATGATAAATCATATTCTCGAGGATTATTTCTCTGCATTCCAATAAGGGAAAGGGAGTTAAGTGTGACAGTGGAGTTAAGTATGCCATAGGTACAAACAGATCATCCAGAGACGGCGTAGTTAAGCAAGCCACAGCTACGCATGGTCGATCTCAACTTATTAACCGTTTTCGATGCCGTGAt                                            <  2:145684/251‑1 (MQ=255)
                                                                                                                                                                                                                      aTCTTTATGTTTTCCTAATTTTACTCATTTTCCTTTTTCTTATTTTATATGCATGATAAATAATATTCTCGAGGTTTATTTCTCTGCATTCCAATATGGGAAAGGAAGTTAAGTGTGACAGTGGAGTTAAGTATGCAAGAGGTACAAACAGATCATCCAGAGCCGGCGGAGTTAAGCAAGCCACAACTACGCATGGTCAACCTCAACTTATTAACCGTTTTCGATGCCGTGATGCAGGGGCAAAACATTat                          >  2:25029/1‑250 (MQ=255)
                                                                                                                                                                                                                             tGTTTTCCTAATTTTACTCATTTTCCTTTTTTTTATTTTATATGCATGATAAATCATATTCTCGAGGATTATTTCTCTGCATTCCAATAAGGGAAAGGGAGTTAAGTGTGACAGGGGAGTTAAGTATGCCAGAGGTACAAACAGATCATCCAGAGACGGCGGAGTTAAGCAAGCCACAGCTACGCATGGTCGATCTCAACTTATTAACCGt                                                           <  2:354066/211‑1 (MQ=255)
                                                                                                                                                                                                                             tGTTTTCCTAATTTTACTCATTTTCCTTTTTCTTATTTTATATGCATGATAAATCATATTCTCGAGGATTATTTCTCTGCATTCCAATAAGGGAAAGGGAGTTAAGTGTGACAGTGGAGTTAAGTATGCCAGAGGTACAAACAGATCATCCAGAGACGGCGGAGTTAAGCAAGCCACAGCTACGCATGGTCGATCTCAACTTATTAACCGt                                                           >  1:354066/1‑211 (MQ=255)
                                                                                                                                                                                                                              gTTTTCCTAATTTTACTCCTTTTCCTTTTTCTTATTTTATATGCTTGATAAATCATCTTCTCGAGGATTCTTTCTCTGCATTCCAATAAGGGAAAGGGAGTTAAGTGTGACAGTGGAGTTAAGTATGCCAGAGGTACAAACAGATCATCCAGCGACGGCGGCGTTAAGCAAGCCACCGCTACGCATGGTCGATCTCAACTTCTTAACCGTTTTCGATGCCGTGATGCAGGGGCAAAACATTACTCGTGCCg                  >  2:438287/1‑251 (MQ=255)
                                                                                                                                                                                                                                     taaTTTTACTCATTTTCCTTTTTCTTATTTTATATGCATGATAACTCATATTCTCGAGGATTATTTCTCTGCATTCCAATAAGGGAAAGGGAGTTAAGTGTGACAGTGGAGTTAAGTATGCCAGAGGAACAAACAGATCATCCAGAGACGGCGGAGTTAAGCAAGCCACAGCTACGCATGGTCGATCTCAACTTATTAACCGTTTTCGATGCCGTGTTGCAGGAGCAAAACATTACTCTTGCCGCTCATGt           >  1:289902/2‑251 (MQ=255)
                                                                                                                                                                                                                                              tCATTTTCCTTTTTCTTATTTTATATGCATGATAAATCATATTCTCGAGGATTATTTCTCTGCATTCCAATAAGGGAAAGGGAGTTAAGTGTGACAGTGGAGTTAAGTATGCCAGAGGTACAAACAGATCATCCAGAGACGGCGGAGTTAAGCAAGCCACAGCTACGCATGGTCGATCTCAACTTATTAACCGTTTTCGATGCCGTGATGCAGGAGCAAAACATTACTCGTGCCGCTCATGTTCTGGGAAt  >  1:77546/1‑251 (MQ=255)
                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                   
TTTGGTGAATATTATTGATCAATTAATGTTAAGAATTAATGCATTAAATATATAAATTAATTATTAAATAAGCACATTTAATCCATTTTGTAGATGATTGAGTATTCGCGGTAGTTATGATTAGATTGTTTTCGCAACAAAAACATTATGGATTATTATGCTGTGGTAAATGACTCATTCCACGGCAATGGATTCTGTTTTTATCAGAACCCGTATCTTTATGTTTTCCGAATTTTACTCATTTTGCTTTTTCTTATTTTATATGCATGATAAATCATATTCTTCAGGATTATTTCTCTGCATTCCAATAAGGGAAAGGGAGTTAAGTGTGACAGTGGAGTTAAGTATGCCAGAGGTACAAACAGATCATCCAGAGACGGCGGAGTTAAGCAAACCACAGCTACGCATGGTCGATCTCAACTTATTAACCGTTTTCGATGCCGTGATGCAGGAGCAAAACATTACTCGTGCCGCTCATGTTCTGGGAAT  >  NC_000913/84022‑84510

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑